File Info

Filename
.command.err
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ff/732fbaf013275f2dd418fb87769128/.command.err
Size
1.8 KB
Task
DAQ:FASTP (1029_0W2-N1-BDNA-1_B23KGCJLT4_1;L007)
Attempt

Content

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Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 18960241
total bases: 2862996391
Q20 bases: 2798539378(97.7486%)
Q30 bases: 2676898264(93.4999%)
Q40 bases: 2676898264(93.4999%)

Read2 before filtering:
total reads: 18960241
total bases: 2862996391
Q20 bases: 2741025118(95.7397%)
Q30 bases: 2538515185(88.6664%)
Q40 bases: 2538515185(88.6664%)

Read1 after filtering:
total reads: 18630117
total bases: 2746023622
Q20 bases: 2695091158(98.1452%)
Q30 bases: 2586361144(94.1857%)
Q40 bases: 2586361144(94.1857%)

Read2 after filtering:
total reads: 18630117
total bases: 2746115318
Q20 bases: 2652858386(96.604%)
Q30 bases: 2474278667(90.101%)
Q40 bases: 2474278667(90.101%)

Filtering result:
reads passed filter: 37260234
reads failed due to low quality: 657524
reads failed due to too many N: 2586
reads failed due to too short: 138
reads with adapter trimmed: 3527636
bases trimmed due to adapters: 134587167

Duplication rate: 11.3823%

Insert size peak (evaluated by paired-end reads): 183

JSON report: 1029_0W2-N1-BDNA-1_B23KGCJLT4_1.fastp.json
HTML report: 1029_0W2-N1-BDNA-1_B23KGCJLT4_1.fastp.html

fastp --in1 1029_0W2-N1-BDNA-1_B23KGCJLT4_1_S48_L007_R1_001.fastq.gz --in2 1029_0W2-N1-BDNA-1_B23KGCJLT4_1_S48_L007_R2_001.fastq.gz --out1 1029_0W2-N1-BDNA-1_B23KGCJLT4_1/1029_0W2-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz --out2 1029_0W2-N1-BDNA-1_B23KGCJLT4_1/1029_0W2-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json 1029_0W2-N1-BDNA-1_B23KGCJLT4_1.fastp.json --html 1029_0W2-N1-BDNA-1_B23KGCJLT4_1.fastp.html --thread 4 
fastp v1.0.1, time used: 236 seconds