Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/90/ecacf41b516744b6fc97c048129cc6/.command.sh
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f2/29f3b4b2b2c0c1b1c882a83133fd5a/NTC_0002_0010_B23KGCJLT4_1/NTC_0002_0010_B23KGCJLT4_1_R2.fastq.gz
Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f2/29f3b4b2b2c0c1b1c882a83133fd5a/NTC_0002_0010_B23KGCJLT4_1/NTC_0002_0010_B23KGCJLT4_1_R1.fastq.gz
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/90/ecacf41b516744b6fc97c048129cc6/.command.run
==> STAGING COMPLETE (7 inputs)
This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NTC_0002_0010_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1774.186 CPU sec, 61.441 real sec
[M::process] read 1114 sequences (168214 bp) in 0.000 CPU sec, 0.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 1, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] skip orientation FR as there are not enough pairs
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 1114 reads in 2.804 CPU sec, 0.099 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
signature: 000c06f2
features: bfebfbff 7ffefbff
extended: f3bfb7ef fb417ffe
amd bits: 2c100800 00000121
brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 1114
overall: 68490436608 mem 1648.474 user 133.736 sys 66.771 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NTC_0002_0010_B23KGCJLT4_1.L001\tPU:L001\tSM:NTC_0002_0010_B23KGCJLT4_1\tLB:NTC_0002_0010_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NTC_0002_0010_B23KGCJLT4_1_R1.fastq.gz NTC_0002_0010_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 66.810 sec; CPU: 1782.213 sec
sorting block 0, #reads = 1171 mem = 651748 peak = 77197312
uniq header count 1
merging 1 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
signature: 000c06f2
features: bfebfbff 7ffefbff
extended: f3bfb7ef fb417ffe
amd bits: 2c100800 00000121
brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 114050
output reads: 1171
bam_mem_sort: 1 calls 0.000 user 0.000 sys 0.000 real
bam_write: 1 calls 0.003 user 0.000 sys 0.003 real
execute: 1 calls 0.050 user 0.147 sys 66.949 real
merge_files: 1 calls 0.032 user 0.119 sys 0.159 real
parse_chunk: 1 calls 0.000 user 0.000 sys 0.001 real
read_chunk: 4 calls 0.001 user 0.000 sys 5.226 real
sort_block: 1 calls 0.006 user 0.000 sys 5.228 real
write_chunk: 1 calls 0.000 user 0.000 sys 0.000 real
overall: 266838016 mem 0.087 user 0.175 sys 66.973 real
ls: cannot access '*.cram': No such file or directory