This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_Blood.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2624.545 CPU sec, 107.392 real sec [M::process] read 125394 sequences (18178356 bp) in 0.179 CPU sec, 0.180 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 45026, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 291, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (315.20, 156.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125394 reads in 22.664 CPU sec, 0.742 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125394 overall: 68161683456 mem 2130.984 user 523.673 sys 115.591 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_Blood.L001\tPU:L001\tSM:Sig_18_Blood\tLB:Sig_18_Blood\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_Blood_R1.fastq.gz Sig_18_Blood_R2.fastq.gz [main] Real time: 115.618 sec; CPU: 2654.660 sec sorting block 0, #reads = 125537 mem = 60403849 peak = 160432128 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7527494 output reads: 125537 bam_mem_sort: 1 calls 0.028 user 0.002 sys 0.030 real bam_write: 1 calls 0.098 user 0.002 sys 0.108 real execute: 1 calls 0.047 user 0.143 sys 116.056 real merge_files: 1 calls 0.028 user 0.118 sys 0.271 real parse_chunk: 2 calls 0.060 user 0.021 sys 0.099 real read_chunk: 8 calls 0.033 user 0.064 sys 7.394 real sort_block: 1 calls 0.154 user 0.076 sys 7.451 real write_chunk: 4 calls 0.009 user 0.013 sys 0.033 real overall: 476327936 mem 1.290 user 0.361 sys 116.072 real ls: cannot access '*.cram': No such file or directory