Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/82/d2362959e3d227d336c30ffeb16d2d/output/Sig_18_tissue_S3_L007_R2_001.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/82/d2362959e3d227d336c30ffeb16d2d/output/Sig_18_tissue_S3_L007_R1_001.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4d/55dfff9c0547139c47cc93fe837d99/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4d/55dfff9c0547139c47cc93fe837d99/.command.run
==> STAGING COMPLETE (4 inputs)

Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 62617
total bases: 9392550
Q20 bases: 9231120(98.2813%)
Q30 bases: 8956037(95.3526%)
Q40 bases: 8956037(95.3526%)

Read2 before filtering:
total reads: 62617
total bases: 9392550
Q20 bases: 9179156(97.7281%)
Q30 bases: 8895552(94.7086%)
Q40 bases: 8895552(94.7086%)

Read1 after filtering:
total reads: 62617
total bases: 8854398
Q20 bases: 8752386(98.8479%)
Q30 bases: 8517115(96.1908%)
Q40 bases: 8517115(96.1908%)

Read2 after filtering:
total reads: 62617
total bases: 8854824
Q20 bases: 8697357(98.2217%)
Q30 bases: 8466364(95.613%)
Q40 bases: 8466364(95.613%)

Filtering result:
reads passed filter: 125234
reads failed due to low quality: 0
reads failed due to too many N: 0
reads failed due to too short: 0
reads with adapter trimmed: 29668
bases trimmed due to adapters: 1075878

Duplication rate: 0.792117%

Insert size peak (evaluated by paired-end reads): 180

JSON report: Sig_18_tissue.fastp.json
HTML report: Sig_18_tissue.fastp.html

fastp --in1 Sig_18_tissue_S3_L007_R1_001.fastq.gz --in2 Sig_18_tissue_S3_L007_R2_001.fastq.gz --out1 Sig_18_tissue/Sig_18_tissue_R1.fastq.gz --out2 Sig_18_tissue/Sig_18_tissue_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json Sig_18_tissue.fastp.json --html Sig_18_tissue.fastp.html --thread 4 
fastp v1.0.1, time used: 1 seconds