cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0004_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1701.534 CPU sec, 296.088 real sec
[M::process] read 686376 sequences (100000148 bp) in 0.912 CPU sec, 0.912 real sec...
[M::process] read 685188 sequences (100000070 bp) in 1.070 CPU sec, 1.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285347, 0, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (308.80, 146.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 203, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362)
[M::mem_pestat] mean and std.dev: (169.85, 42.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 439)
[M::mem_pestat] skip orientation RR
[M::process] read 684468 sequences (100000024 bp) in 1.033 CPU sec, 1.050 real sec...
[M::process] read 684042 sequences (100000079 bp) in 1.049 CPU sec, 1.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285219, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 295, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (313.64, 148.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686376 reads in 130.321 CPU sec, 4.163 real sec
[M::mem_process_seqs] Processed 685188 reads in 78.939 CPU sec, 2.527 real sec
sorting block 0, #reads = 1074034 mem = 536878824 peak = 611856384
[M::process] read 683324 sequences (100000091 bp) in 1.416 CPU sec, 1.492 real sec...
[M::process] read 682670 sequences (100000139 bp) in 1.060 CPU sec, 1.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284578, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (326.63, 155.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 305, 1093)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3051)
[M::mem_pestat] mean and std.dev: (572.08, 442.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4030)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683324 reads in 68.713 CPU sec, 2.211 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284593, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 153, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 303)
[M::mem_pestat] mean and std.dev: (171.73, 65.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 433)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (319.07, 150.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284461, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 304, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (323.43, 154.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682650 sequences (100000127 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_process_seqs] Processed 684468 reads in 195.216 CPU sec, 6.239 real sec
sorting block 1, #reads = 1072865 mem = 536878129 peak = 739258368
[M::process] read 685426 sequences (100000132 bp) in 1.121 CPU sec, 1.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283461, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 309, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (328.01, 155.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 269, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (216.90, 101.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 749)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682670 reads in 71.630 CPU sec, 2.306 real sec
sorting block 2, #reads = 1071706 mem = 536880330 peak = 863961088
[M::mem_process_seqs] Processed 684042 reads in 241.105 CPU sec, 7.755 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284007, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (326.50, 155.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685458 sequences (100000259 bp) in 1.427 CPU sec, 1.527 real sec...
[M::process] read 684214 sequences (100000188 bp) in 1.110 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285174, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (109, 226, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (190.50, 75.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (315.67, 151.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 682650 reads in 140.639 CPU sec, 4.512 real sec
sorting block 3, #reads = 1071481 mem = 536880912 peak = 863961088
[M::mem_process_seqs] Processed 685426 reads in 80.398 CPU sec, 2.576 real sec
sorting block 4, #reads = 1072736 mem = 536879597 peak = 863961088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285432, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 298, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (316.14, 150.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683444 sequences (100000206 bp) in 1.753 CPU sec, 1.880 real sec...
[M::process] read 683216 sequences (100000273 bp) in 1.160 CPU sec, 1.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285128, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (320.02, 151.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685458 reads in 128.574 CPU sec, 4.116 real sec
[M::mem_process_seqs] Processed 684214 reads in 80.035 CPU sec, 2.562 real sec
sorting block 5, #reads = 1073267 mem = 536879514 peak = 863961088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284229, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 306, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (324.55, 153.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683054 sequences (100000154 bp) in 1.756 CPU sec, 1.877 real sec...
[M::process] read 683296 sequences (100000173 bp) in 1.200 CPU sec, 1.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284569, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (326.01, 154.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683444 reads in 129.078 CPU sec, 4.132 real sec
[M::mem_process_seqs] Processed 683216 reads in 80.438 CPU sec, 2.575 real sec
sorting block 6, #reads = 1072624 mem = 536881313 peak = 863961088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284316, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (326.65, 155.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685092 sequences (100000185 bp) in 1.907 CPU sec, 2.033 real sec...
[M::process] read 685164 sequences (100000238 bp) in 1.210 CPU sec, 1.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284380, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (325.86, 156.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 231, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 343)
[M::mem_pestat] mean and std.dev: (224.88, 21.06)
[M::mem_pestat] low and high boundaries for proper pairs: (79, 387)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683054 reads in 130.663 CPU sec, 4.183 real sec
[M::mem_process_seqs] Processed 683296 reads in 81.423 CPU sec, 2.598 real sec
sorting block 7, #reads = 1071464 mem = 536878395 peak = 863961088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284671, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 297, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (317.59, 152.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 176, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317)
[M::mem_pestat] mean and std.dev: (159.40, 54.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 386)
[M::mem_pestat] skip orientation RR
[M::process] read 684258 sequences (100000198 bp) in 1.955 CPU sec, 2.086 real sec...
[M::process] read 683330 sequences (100000052 bp) in 1.230 CPU sec, 1.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284712, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 297, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (316.27, 150.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685092 reads in 130.986 CPU sec, 4.190 real sec
sorting block 8, #reads = 1072707 mem = 536877620 peak = 863961088
[M::mem_process_seqs] Processed 685164 reads in 81.063 CPU sec, 2.590 real sec
sorting block 9, #reads = 1072273 mem = 536878885 peak = 939393024
[M::process] read 682636 sequences (100000042 bp) in 2.037 CPU sec, 2.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284429, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682708 sequences (100000020 bp) in 1.270 CPU sec, 1.266 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (325.49, 154.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683330 reads in 71.669 CPU sec, 2.275 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284589, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (320.69, 151.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283630, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 309, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (328.00, 155.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683300 sequences (100000140 bp) in 1.324 CPU sec, 1.329 real sec...
[M::mem_process_seqs] Processed 684258 reads in 201.918 CPU sec, 6.460 real sec
sorting block 10, #reads = 1072098 mem = 536879023 peak = 939393024
[M::mem_process_seqs] Processed 682636 reads in 94.524 CPU sec, 3.041 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283632, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (327.48, 156.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 11, #reads = 1070996 mem = 536881121 peak = 939393024
[M::process] read 684024 sequences (100000084 bp) in 2.156 CPU sec, 2.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284106, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (324.72, 155.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685128 sequences (100000165 bp) in 1.240 CPU sec, 1.234 real sec...
[M::mem_process_seqs] Processed 682708 reads in 131.402 CPU sec, 4.203 real sec
[M::mem_process_seqs] Processed 683300 reads in 81.731 CPU sec, 2.604 real sec
sorting block 12, #reads = 1072135 mem = 536879035 peak = 939393024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284029, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (322.41, 155.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684078 sequences (100000288 bp) in 1.824 CPU sec, 1.995 real sec...
[M::process] read 683014 sequences (100000137 bp) in 1.270 CPU sec, 1.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284976, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (316.24, 151.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684024 reads in 135.424 CPU sec, 4.354 real sec
[M::mem_process_seqs] Processed 685128 reads in 83.700 CPU sec, 2.677 real sec
sorting block 13, #reads = 1071979 mem = 536886857 peak = 939393024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284362, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (321.63, 153.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682642 sequences (100000257 bp) in 2.131 CPU sec, 2.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284076, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (325.38, 153.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683128 sequences (100000146 bp) in 1.280 CPU sec, 1.281 real sec...
[M::mem_process_seqs] Processed 684078 reads in 132.662 CPU sec, 4.247 real sec
[M::mem_process_seqs] Processed 683014 reads in 81.752 CPU sec, 2.605 real sec
sorting block 14, #reads = 1073375 mem = 536878144 peak = 939393024
sorting block 15, #reads = 1071523 mem = 536878102 peak = 939393024
[M::process] read 683404 sequences (100000114 bp) in 2.212 CPU sec, 2.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284121, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (324.10, 154.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683128 reads in 71.020 CPU sec, 2.238 real sec
[M::process] read 684028 sequences (100000205 bp) in 1.336 CPU sec, 1.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284004, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (327.11, 154.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284458, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (322.62, 154.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682642 reads in 197.026 CPU sec, 6.274 real sec
[M::process] read 688306 sequences (100000197 bp) in 1.634 CPU sec, 1.639 real sec...
[M::mem_process_seqs] Processed 683404 reads in 92.403 CPU sec, 2.937 real sec
sorting block 16, #reads = 1070801 mem = 536878804 peak = 939393024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283897, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (321.77, 155.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686092 sequences (100000035 bp) in 2.229 CPU sec, 2.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286597, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (306.70, 150.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 243, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976)
[M::mem_pestat] mean and std.dev: (421.85, 354.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2580)
[M::mem_pestat] skip orientation RR
[M::process] read 685042 sequences (100000028 bp) in 1.240 CPU sec, 1.238 real sec...
[M::mem_process_seqs] Processed 684028 reads in 129.831 CPU sec, 4.132 real sec
sorting block 17, #reads = 1071172 mem = 536880172 peak = 939393024
[M::mem_process_seqs] Processed 688306 reads in 82.974 CPU sec, 2.629 real sec
sorting block 18, #reads = 1072073 mem = 536878195 peak = 996061184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285216, 0, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 295, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (313.90, 149.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 2036, 2036)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5708)
[M::mem_pestat] mean and std.dev: (1241.43, 919.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7544)
[M::mem_pestat] skip orientation RR
[M::process] read 685028 sequences (100000244 bp) in 2.128 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285381, 7, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (261, 339, 2620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7338)
[M::mem_pestat] mean and std.dev: (1358.27, 1575.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9697)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 298, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (317.79, 151.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684250 sequences (100000162 bp) in 1.280 CPU sec, 1.275 real sec...
[M::mem_process_seqs] Processed 686092 reads in 130.100 CPU sec, 4.139 real sec
[M::mem_process_seqs] Processed 685042 reads in 81.831 CPU sec, 2.598 real sec
sorting block 19, #reads = 1071968 mem = 536880760 peak = 996061184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285610, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 334, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (361.54, 231.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 300, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (320.04, 153.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684038 sequences (100000177 bp) in 2.075 CPU sec, 2.190 real sec...
[M::process] read 684260 sequences (100000213 bp) in 1.300 CPU sec, 1.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284717, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 297, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (315.24, 147.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685028 reads in 135.900 CPU sec, 4.331 real sec
[M::mem_process_seqs] Processed 684250 reads in 82.738 CPU sec, 2.632 real sec
sorting block 20, #reads = 1070240 mem = 536877563 peak = 996061184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284700, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 299, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (316.15, 146.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687500 sequences (100000134 bp) in 2.174 CPU sec, 2.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284587, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 297, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (316.44, 149.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685944 sequences (100000029 bp) in 1.300 CPU sec, 1.301 real sec...
[M::mem_process_seqs] Processed 684038 reads in 132.548 CPU sec, 4.223 real sec
sorting block 21, #reads = 1070633 mem = 536878411 peak = 996061184
[M::mem_process_seqs] Processed 684260 reads in 84.077 CPU sec, 2.672 real sec
sorting block 22, #reads = 1069531 mem = 536885009 peak = 996061184
[M::process] read 685236 sequences (100000060 bp) in 2.162 CPU sec, 2.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285671, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 292, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (311.27, 147.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684912 sequences (100000239 bp) in 1.320 CPU sec, 1.323 real sec...
[M::mem_process_seqs] Processed 685944 reads in 73.805 CPU sec, 2.327 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285651, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 285, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (304.63, 145.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285091, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 216, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516)
[M::mem_pestat] mean and std.dev: (176.60, 68.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 646)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (315.35, 149.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683992 sequences (100000209 bp) in 1.595 CPU sec, 1.599 real sec...
[M::mem_process_seqs] Processed 687500 reads in 206.764 CPU sec, 6.574 real sec
[M::mem_process_seqs] Processed 685236 reads in 95.733 CPU sec, 3.053 real sec
sorting block 23, #reads = 1071838 mem = 536883151 peak = 996061184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284889, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 184, 184)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 302)
[M::mem_pestat] mean and std.dev: (173.25, 55.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 395)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 298, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (315.82, 148.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 305, 747)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1745)
[M::mem_pestat] mean and std.dev: (388.20, 239.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2244)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 24, #reads = 1070680 mem = 536878952 peak = 996061184
[M::process] read 683402 sequences (100000095 bp) in 2.066 CPU sec, 2.227 real sec...
[M::process] read 685082 sequences (100000044 bp) in 1.290 CPU sec, 1.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285161, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 300, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (318.65, 149.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684912 reads in 143.153 CPU sec, 4.577 real sec
[M::mem_process_seqs] Processed 683992 reads in 92.199 CPU sec, 2.932 real sec
sorting block 25, #reads = 1067196 mem = 536875664 peak = 996061184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284072, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (321.25, 150.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (97, 215, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (171.89, 88.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RR
[M::process] read 686530 sequences (100000229 bp) in 2.133 CPU sec, 2.272 real sec...
[M::process] read 685266 sequences (100000163 bp) in 1.290 CPU sec, 1.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285117, 0, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 190, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455)
[M::mem_pestat] mean and std.dev: (184.80, 84.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 569)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (317.61, 152.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 351, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (282.56, 88.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683402 reads in 137.441 CPU sec, 4.378 real sec
[M::mem_process_seqs] Processed 685082 reads in 87.374 CPU sec, 2.785 real sec
sorting block 26, #reads = 1068369 mem = 536881302 peak = 996061184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285500, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 289, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (308.53, 147.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684734 sequences (100000266 bp) in 2.129 CPU sec, 2.249 real sec...
[M::process] read 684134 sequences (100000046 bp) in 1.290 CPU sec, 1.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284980, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 177, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (184.90, 64.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (312.63, 147.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686530 reads in 137.465 CPU sec, 4.379 real sec
[M::mem_process_seqs] Processed 685266 reads in 84.251 CPU sec, 2.678 real sec
sorting block 27, #reads = 1069023 mem = 536879461 peak = 996061184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285561, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (316.42, 149.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683198 sequences (100000212 bp) in 2.138 CPU sec, 2.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285055, 0, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (319.39, 150.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 467, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243)
[M::mem_pestat] mean and std.dev: (341.00, 210.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1610)
[M::mem_pestat] skip orientation RR
[M::process] read 683126 sequences (100000244 bp) in 1.320 CPU sec, 1.314 real sec...
[M::mem_process_seqs] Processed 684734 reads in 135.095 CPU sec, 4.303 real sec
sorting block 28, #reads = 1071780 mem = 536878295 peak = 996061184
[M::mem_process_seqs] Processed 684134 reads in 85.191 CPU sec, 2.707 real sec
sorting block 29, #reads = 1069165 mem = 536878807 peak = 1009668096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284547, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (322.82, 151.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685118 sequences (100000196 bp) in 2.173 CPU sec, 2.302 real sec...
[M::process] read 686746 sequences (100000091 bp) in 1.340 CPU sec, 1.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284378, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (322.64, 152.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683198 reads in 142.585 CPU sec, 4.544 real sec
[M::mem_process_seqs] Processed 683126 reads in 91.381 CPU sec, 2.910 real sec
sorting block 30, #reads = 1066329 mem = 536881512 peak = 1009668096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285132, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (315.30, 152.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685314 sequences (100000257 bp) in 1.950 CPU sec, 2.072 real sec...
[M::process] read 684328 sequences (100000250 bp) in 1.290 CPU sec, 1.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285823, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 183, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (206.60, 89.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (310.16, 149.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 685118 reads in 141.459 CPU sec, 4.513 real sec
[M::mem_process_seqs] Processed 686746 reads in 87.091 CPU sec, 2.771 real sec
sorting block 31, #reads = 1067127 mem = 536881295 peak = 1009668096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285871, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 296, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (316.56, 151.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683660 sequences (100000172 bp) in 2.217 CPU sec, 2.356 real sec...
[M::process] read 683288 sequences (100000200 bp) in 1.290 CPU sec, 1.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284791, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 303, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (322.65, 153.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685314 reads in 140.424 CPU sec, 4.484 real sec
sorting block 32, #reads = 1071048 mem = 536882448 peak = 1009668096
[M::mem_process_seqs] Processed 684328 reads in 91.390 CPU sec, 2.910 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284533, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 170, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488)
[M::mem_pestat] mean and std.dev: (187.80, 55.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 598)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (323.09, 153.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 218, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (188, 263)
[M::mem_pestat] mean and std.dev: (220.14, 5.25)
[M::mem_pestat] low and high boundaries for proper pairs: (173, 278)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 33, #reads = 1068614 mem = 536879376 peak = 1009668096
[M::process] read 683278 sequences (100000216 bp) in 2.070 CPU sec, 2.217 real sec...
[M::process] read 373894 sequences (54729485 bp) in 0.840 CPU sec, 0.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284404, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 319, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (278.29, 114.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 300, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (319.21, 149.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 683660 reads in 142.857 CPU sec, 4.571 real sec
[M::mem_process_seqs] Processed 683288 reads in 88.535 CPU sec, 2.828 real sec
sorting block 34, #reads = 1067206 mem = 536880145 peak = 1009668096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284400, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 186, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (218.33, 124.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 300, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (319.46, 150.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 155155, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (322.47, 152.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683278 reads in 106.122 CPU sec, 3.455 real sec
[M::mem_process_seqs] Processed 373894 reads in 51.347 CPU sec, 1.670 real sec
sorting block 35, #reads = 1068531 mem = 536880725 peak = 1009668096
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 38698458
overall: 69510168576 mem 5543.668 user 272.597 sys 435.999 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0004_B23KGCJLT4_1.L007\tPU:L007\tSM:NA12878_c_0002_gDNA_0004_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0004_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0004_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0004_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 436.034 sec; CPU: 5816.268 sec
sorting block 36, #reads = 335582 mem = 168695674 peak = 1009668096
uniq header count 1
merging 37 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2039306720
output reads: 38886169
bam_mem_sort: 37 calls 13.425 user 0.187 sys 13.691 real
bam_write: 37 calls 19.759 user 0.698 sys 20.665 real
execute: 1 calls 7.087 user 0.384 sys 446.737 real
merge_files: 1 calls 7.067 user 0.365 sys 10.284 real
parse_chunk: 582 calls 18.444 user 4.685 sys 23.517 real
read_chunk: 1572 calls 12.537 user 13.774 sys 138.802 real
sort_block: 1 calls 10.592 user 13.341 sys 135.271 real
write_chunk: 988 calls 6.779 user 0.220 sys 9.663 real
overall: 2366078976 mem 393.569 user 24.584 sys 446.755 real
ls: cannot access '*.cram': No such file or directory