.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_eZ4-N1-BDNA-1_B23KGCJLT4_1;L002;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e7/3a87d2d94f04fdf529cb1a33e6c85b/.command.err
Size: 68.1 KB
Task: DAQ:SENTIEON_BWAMEM (659_eZ4-N1-BDNA-1_B23KGCJLT4_1;L002;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_eZ4-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1723.189 CPU sec, 1257.523 real sec
[M::process] read 684502 sequences (100000197 bp) in 0.955 CPU sec, 0.959 real sec...
[M::process] read 682836 sequences (100000265 bp) in 1.340 CPU sec, 1.365 real sec...
[M::process] read 682324 sequences (100000210 bp) in 1.234 CPU sec, 1.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282631, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 312, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (329.51, 158.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681596 sequences (100000049 bp) in 1.236 CPU sec, 1.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282667, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (334.82, 158.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282056, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (341.29, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684502 reads in 216.314 CPU sec, 7.001 real sec
[M::mem_process_seqs] Processed 682836 reads in 143.475 CPU sec, 4.689 real sec
[M::mem_process_seqs] Processed 682324 reads in 155.256 CPU sec, 5.089 real sec
sorting block 0, #reads = 1085100 mem = 536880284 peak = 597405696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282044, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (540, 540, 1293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2799)
[M::mem_pestat] mean and std.dev: (588.44, 350.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3552)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682676 sequences (100000130 bp) in 1.894 CPU sec, 2.068 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (213, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (344.03, 162.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682826 sequences (100000136 bp) in 1.343 CPU sec, 1.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281741, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (335.54, 161.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681596 reads in 131.379 CPU sec, 4.213 real sec
sorting block 1, #reads = 1084879 mem = 536878406 peak = 841199616
[M::mem_process_seqs] Processed 682676 reads in 84.063 CPU sec, 2.658 real sec
[M::process] read 683312 sequences (100000276 bp) in 1.416 CPU sec, 1.457 real sec...
sorting block 2, #reads = 1083815 mem = 536879016 peak = 896753664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281688, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 314, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (333.40, 160.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682996 sequences (100000119 bp) in 1.646 CPU sec, 1.782 real sec...
[M::process] read 682806 sequences (100000236 bp) in 1.230 CPU sec, 1.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282275, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 309, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (330.27, 160.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682826 reads in 132.238 CPU sec, 4.225 real sec
[M::mem_process_seqs] Processed 683312 reads in 83.284 CPU sec, 2.664 real sec
sorting block 3, #reads = 1084085 mem = 536882744 peak = 896753664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281851, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 310, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (331.98, 160.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682090 sequences (100000295 bp) in 1.821 CPU sec, 1.932 real sec...
[M::process] read 684216 sequences (100000046 bp) in 1.270 CPU sec, 1.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281910, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 312, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (334.76, 163.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682996 reads in 133.388 CPU sec, 4.257 real sec
[M::mem_process_seqs] Processed 682806 reads in 83.825 CPU sec, 2.676 real sec
sorting block 4, #reads = 1084322 mem = 536878448 peak = 896753664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281618, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (339.28, 166.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682800 sequences (100000188 bp) in 1.897 CPU sec, 2.010 real sec...
[M::process] read 682104 sequences (100000087 bp) in 1.260 CPU sec, 1.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282970, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 315, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (332.67, 160.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682090 reads in 133.721 CPU sec, 4.264 real sec
[M::mem_process_seqs] Processed 684216 reads in 83.162 CPU sec, 2.653 real sec
sorting block 5, #reads = 1084705 mem = 536878180 peak = 896753664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282149, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (337.79, 160.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681398 sequences (100000183 bp) in 2.016 CPU sec, 2.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282615, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682602 sequences (100000047 bp) in 1.320 CPU sec, 1.322 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (212, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (344.53, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682800 reads in 131.499 CPU sec, 4.195 real sec
sorting block 6, #reads = 1085905 mem = 536882799 peak = 896753664
[M::mem_process_seqs] Processed 682104 reads in 83.095 CPU sec, 2.644 real sec
sorting block 7, #reads = 1085025 mem = 536879268 peak = 923914240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282113, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (345.35, 162.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682582 sequences (100000165 bp) in 2.024 CPU sec, 2.158 real sec...
[M::process] read 682970 sequences (100000071 bp) in 1.350 CPU sec, 1.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281871, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (337.27, 161.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681398 reads in 132.176 CPU sec, 4.213 real sec
[M::mem_process_seqs] Processed 682602 reads in 83.766 CPU sec, 2.665 real sec
sorting block 8, #reads = 1085155 mem = 536877773 peak = 923914240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282251, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (335.73, 160.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683424 sequences (100000292 bp) in 2.185 CPU sec, 2.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282271, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (333.77, 161.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682558 sequences (100000201 bp) in 1.360 CPU sec, 1.365 real sec...
[M::mem_process_seqs] Processed 682582 reads in 134.940 CPU sec, 4.299 real sec
[M::mem_process_seqs] Processed 682970 reads in 85.314 CPU sec, 2.711 real sec
sorting block 9, #reads = 1083909 mem = 536878996 peak = 923914240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282372, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 312, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (333.93, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682350 sequences (100000115 bp) in 2.199 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281605, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 312, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (335.34, 163.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684040 sequences (100000222 bp) in 1.390 CPU sec, 1.394 real sec...
[M::mem_process_seqs] Processed 683424 reads in 135.066 CPU sec, 4.303 real sec
sorting block 10, #reads = 1083916 mem = 536879417 peak = 923914240
[M::mem_process_seqs] Processed 682558 reads in 85.145 CPU sec, 2.702 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281895, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 317, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (339.70, 166.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 11, #reads = 1083990 mem = 536879086 peak = 923914240
[M::process] read 683240 sequences (100000179 bp) in 2.253 CPU sec, 2.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282308, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 314, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (333.26, 161.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682468 sequences (100000234 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_process_seqs] Processed 682350 reads in 135.850 CPU sec, 4.329 real sec
[M::mem_process_seqs] Processed 684040 reads in 85.553 CPU sec, 2.725 real sec
sorting block 12, #reads = 1083534 mem = 536878273 peak = 923914240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282420, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (340.17, 163.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682494 sequences (100000246 bp) in 2.286 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282435, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (342.01, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683248 sequences (100000208 bp) in 1.405 CPU sec, 1.412 real sec...
[M::mem_process_seqs] Processed 683240 reads in 132.950 CPU sec, 4.245 real sec
[M::mem_process_seqs] Processed 682468 reads in 84.195 CPU sec, 2.676 real sec
sorting block 13, #reads = 1085381 mem = 536881103 peak = 923914240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282794, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (342.34, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682418 sequences (100000026 bp) in 2.356 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282172, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 317, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (336.10, 162.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682494 reads in 134.316 CPU sec, 4.281 real sec
[M::process] read 682394 sequences (100000276 bp) in 1.410 CPU sec, 1.409 real sec...
[M::mem_process_seqs] Processed 683248 reads in 85.779 CPU sec, 2.719 real sec
sorting block 14, #reads = 1084399 mem = 536882046 peak = 923914240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281821, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 320, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (338.43, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682820 sequences (100000060 bp) in 2.415 CPU sec, 2.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281942, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (337.94, 163.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682808 sequences (100000100 bp) in 1.410 CPU sec, 1.405 real sec...
[M::mem_process_seqs] Processed 682418 reads in 135.703 CPU sec, 4.323 real sec
sorting block 15, #reads = 1084876 mem = 536888533 peak = 923914240
[M::mem_process_seqs] Processed 682394 reads in 85.670 CPU sec, 2.717 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282613, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 311, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (334.71, 163.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1084485 mem = 536876020 peak = 930824192
[M::process] read 682462 sequences (100000046 bp) in 2.338 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281800, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 315, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (337.37, 165.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684332 sequences (100000081 bp) in 1.400 CPU sec, 1.394 real sec...
[M::mem_process_seqs] Processed 682820 reads in 135.167 CPU sec, 4.306 real sec
[M::mem_process_seqs] Processed 682808 reads in 85.907 CPU sec, 2.726 real sec
sorting block 17, #reads = 1084699 mem = 536880039 peak = 930824192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281440, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 317, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (339.70, 166.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683098 sequences (100000015 bp) in 2.036 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282884, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 312, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (334.07, 164.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682558 sequences (100000074 bp) in 1.380 CPU sec, 1.375 real sec...
[M::mem_process_seqs] Processed 682462 reads in 136.500 CPU sec, 4.364 real sec
[M::mem_process_seqs] Processed 684332 reads in 86.390 CPU sec, 2.741 real sec
sorting block 18, #reads = 1083921 mem = 536879213 peak = 930824192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282557, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 320, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (339.57, 164.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682312 sequences (100000151 bp) in 2.359 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282504, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 321, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (337.67, 160.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682476 sequences (100000043 bp) in 1.400 CPU sec, 1.400 real sec...
[M::mem_process_seqs] Processed 683098 reads in 133.634 CPU sec, 4.262 real sec
[M::mem_process_seqs] Processed 682558 reads in 84.859 CPU sec, 2.694 real sec
sorting block 19, #reads = 1085187 mem = 536878337 peak = 930824192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281809, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 322, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (339.06, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682240 sequences (100000066 bp) in 2.457 CPU sec, 2.614 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282225, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (336.25, 160.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682312 reads in 134.448 CPU sec, 4.288 real sec
[M::process] read 682828 sequences (100000170 bp) in 1.427 CPU sec, 1.432 real sec...
sorting block 20, #reads = 1085806 mem = 536878413 peak = 930824192
[M::mem_process_seqs] Processed 682476 reads in 85.561 CPU sec, 2.715 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282029, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (336.28, 161.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1084650 mem = 536878518 peak = 958005248
[M::process] read 682698 sequences (100000213 bp) in 2.373 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281992, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (333.59, 160.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682466 sequences (100000174 bp) in 1.400 CPU sec, 1.403 real sec...
[M::mem_process_seqs] Processed 682240 reads in 137.480 CPU sec, 4.387 real sec
[M::mem_process_seqs] Processed 682828 reads in 87.160 CPU sec, 2.761 real sec
sorting block 22, #reads = 1084258 mem = 536879291 peak = 958005248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281807, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (333.91, 161.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682574 sequences (100000246 bp) in 2.397 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281932, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (335.01, 162.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685478 sequences (100000068 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 682698 reads in 136.987 CPU sec, 4.361 real sec
[M::mem_process_seqs] Processed 682466 reads in 86.311 CPU sec, 2.735 real sec
sorting block 23, #reads = 1084105 mem = 536877748 peak = 958005248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282167, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (336.68, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684422 sequences (100000068 bp) in 2.376 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283328, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 304, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (328.97, 164.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683432 sequences (100000117 bp) in 1.360 CPU sec, 1.361 real sec...
[M::mem_process_seqs] Processed 682574 reads in 135.504 CPU sec, 4.315 real sec
[M::mem_process_seqs] Processed 685478 reads in 85.458 CPU sec, 2.710 real sec
sorting block 24, #reads = 1083932 mem = 536877163 peak = 958005248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283084, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 313, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (332.31, 161.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682862 sequences (100000239 bp) in 2.346 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282634, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (335.59, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684422 reads in 130.229 CPU sec, 4.148 real sec
[M::process] read 683894 sequences (100000293 bp) in 1.460 CPU sec, 1.470 real sec...
sorting block 25, #reads = 1086147 mem = 536881487 peak = 958005248
[M::mem_process_seqs] Processed 683432 reads in 82.862 CPU sec, 2.625 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282488, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (337.04, 160.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1085708 mem = 536878017 peak = 1025531904
[M::process] read 684096 sequences (100000252 bp) in 2.253 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282728, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 309, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (327.54, 156.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682862 reads in 129.637 CPU sec, 4.135 real sec
[M::mem_process_seqs] Processed 683894 reads in 83.064 CPU sec, 2.627 real sec
[M::process] read 683704 sequences (100000235 bp) in 1.739 CPU sec, 1.787 real sec...
sorting block 27, #reads = 1084818 mem = 536879955 peak = 1025531904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283224, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (327.73, 157.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683616 sequences (100000071 bp) in 2.166 CPU sec, 2.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282797, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (326.35, 155.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684096 reads in 131.961 CPU sec, 4.198 real sec
[M::process] read 683388 sequences (100000281 bp) in 1.477 CPU sec, 1.491 real sec...
[M::mem_process_seqs] Processed 683704 reads in 84.244 CPU sec, 2.660 real sec
sorting block 28, #reads = 1085769 mem = 536879361 peak = 1025531904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282370, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (326.52, 156.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683274 sequences (100000082 bp) in 2.379 CPU sec, 2.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282354, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 305, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (324.37, 154.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683616 reads in 132.911 CPU sec, 4.226 real sec
[M::process] read 684754 sequences (100000222 bp) in 1.514 CPU sec, 1.532 real sec...
[M::mem_process_seqs] Processed 683388 reads in 84.823 CPU sec, 2.677 real sec
sorting block 29, #reads = 1085355 mem = 536879644 peak = 1025531904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281940, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (325.30, 154.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684156 sequences (100000078 bp) in 2.234 CPU sec, 2.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282877, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 304, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (326.34, 159.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683394 sequences (100000099 bp) in 1.370 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 683274 reads in 135.635 CPU sec, 4.314 real sec
sorting block 30, #reads = 1084299 mem = 536886006 peak = 1025531904
[M::mem_process_seqs] Processed 684754 reads in 86.119 CPU sec, 2.724 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283106, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 312, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (329.69, 158.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1082765 mem = 536879506 peak = 1062555648
[M::process] read 682734 sequences (100000233 bp) in 2.344 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282481, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (330.50, 157.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684156 reads in 129.606 CPU sec, 4.121 real sec
[M::process] read 683686 sequences (100000199 bp) in 1.513 CPU sec, 1.522 real sec...
[M::mem_process_seqs] Processed 683394 reads in 82.092 CPU sec, 2.593 real sec
sorting block 32, #reads = 1086389 mem = 536884616 peak = 1062555648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282111, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (333.62, 157.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684086 sequences (100000198 bp) in 2.199 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282795, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 310, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (326.73, 154.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682734 reads in 129.001 CPU sec, 4.100 real sec
[M::process] read 683604 sequences (100000169 bp) in 1.418 CPU sec, 1.416 real sec...
[M::mem_process_seqs] Processed 683686 reads in 82.294 CPU sec, 2.603 real sec
sorting block 33, #reads = 1085097 mem = 536879883 peak = 1062555648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282922, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 307, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (324.92, 154.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683238 sequences (100000256 bp) in 2.337 CPU sec, 2.469 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282760, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 309, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (326.69, 155.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683034 sequences (100000139 bp) in 1.400 CPU sec, 1.393 real sec...
[M::mem_process_seqs] Processed 684086 reads in 136.554 CPU sec, 4.339 real sec
sorting block 34, #reads = 1084969 mem = 536886232 peak = 1062555648
[M::mem_process_seqs] Processed 683604 reads in 83.746 CPU sec, 2.653 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282497, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 309, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (327.96, 156.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1084438 mem = 536880683 peak = 1062555648
[M::process] read 682884 sequences (100000073 bp) in 2.038 CPU sec, 2.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282218, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 312, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (331.32, 159.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683238 reads in 130.679 CPU sec, 4.160 real sec
[M::process] read 683916 sequences (100000150 bp) in 1.493 CPU sec, 1.501 real sec...
[M::mem_process_seqs] Processed 683034 reads in 84.011 CPU sec, 2.655 real sec
sorting block 36, #reads = 1084593 mem = 536879490 peak = 1062555648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281835, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (332.20, 160.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684352 sequences (100000254 bp) in 2.539 CPU sec, 2.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282354, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 307, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (329.29, 160.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682884 reads in 140.262 CPU sec, 4.463 real sec
[M::process] read 683438 sequences (100000106 bp) in 1.483 CPU sec, 1.499 real sec...
[M::mem_process_seqs] Processed 683916 reads in 92.323 CPU sec, 2.918 real sec
sorting block 37, #reads = 1083067 mem = 536877906 peak = 1062555648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282928, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 310, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (328.81, 158.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682912 sequences (100000011 bp) in 2.148 CPU sec, 2.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282189, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 314, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (331.29, 158.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684352 reads in 130.939 CPU sec, 4.172 real sec
[M::process] read 683848 sequences (100000117 bp) in 1.484 CPU sec, 1.502 real sec...
[M::mem_process_seqs] Processed 683438 reads in 84.196 CPU sec, 2.661 real sec
sorting block 38, #reads = 1081916 mem = 536884805 peak = 1062555648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282971, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 316, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (334.07, 159.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684200 sequences (100000107 bp) in 2.410 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283058, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (326.96, 156.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684072 sequences (100000222 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 682912 reads in 141.738 CPU sec, 4.506 real sec
sorting block 39, #reads = 1085266 mem = 536884802 peak = 1062555648
[M::mem_process_seqs] Processed 683848 reads in 94.617 CPU sec, 2.994 real sec
sorting block 40, #reads = 1084439 mem = 536880073 peak = 1062555648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282700, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 303, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (323.38, 154.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683562 sequences (100000071 bp) in 2.370 CPU sec, 2.493 real sec...
[M::process] read 682828 sequences (100000204 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283008, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 307, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (325.71, 155.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684200 reads in 162.696 CPU sec, 5.176 real sec
[M::mem_process_seqs] Processed 684072 reads in 99.812 CPU sec, 3.176 real sec
sorting block 41, #reads = 1072217 mem = 536882979 peak = 1062555648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283044, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 307, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (325.63, 155.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683200 sequences (100000193 bp) in 2.426 CPU sec, 2.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282828, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (331.19, 158.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685344 sequences (100000285 bp) in 1.420 CPU sec, 1.425 real sec...
[M::mem_process_seqs] Processed 683562 reads in 142.844 CPU sec, 4.535 real sec
[M::mem_process_seqs] Processed 682828 reads in 88.988 CPU sec, 2.819 real sec
sorting block 42, #reads = 1080824 mem = 536885285 peak = 1062555648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282191, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 310, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (331.35, 160.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684610 sequences (100000157 bp) in 2.316 CPU sec, 2.448 real sec...
[M::process] read 683242 sequences (100000158 bp) in 1.380 CPU sec, 1.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283187, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 303, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (324.98, 159.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683200 reads in 153.298 CPU sec, 4.883 real sec
[M::mem_process_seqs] Processed 685344 reads in 94.373 CPU sec, 3.006 real sec
sorting block 43, #reads = 1078394 mem = 536880458 peak = 1062555648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283132, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 307, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (328.57, 160.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683190 sequences (100000282 bp) in 2.436 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282644, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (330.95, 158.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684610 reads in 133.387 CPU sec, 4.236 real sec
[M::process] read 683370 sequences (100000108 bp) in 1.416 CPU sec, 1.425 real sec...
sorting block 44, #reads = 1078648 mem = 536878371 peak = 1062555648
[M::mem_process_seqs] Processed 683242 reads in 83.626 CPU sec, 2.642 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282618, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 309, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (326.30, 153.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1084596 mem = 536883277 peak = 1113051136
[M::process] read 682946 sequences (100000025 bp) in 2.264 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282878, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 310, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (327.82, 155.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682702 sequences (100000164 bp) in 1.400 CPU sec, 1.395 real sec...
[M::mem_process_seqs] Processed 683190 reads in 139.044 CPU sec, 4.423 real sec
[M::mem_process_seqs] Processed 683370 reads in 93.144 CPU sec, 2.953 real sec
sorting block 46, #reads = 1083177 mem = 536877304 peak = 1113051136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282825, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 314, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (332.10, 158.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682646 sequences (100000269 bp) in 2.445 CPU sec, 2.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281607, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (332.71, 159.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682946 reads in 133.596 CPU sec, 4.241 real sec
[M::process] read 681952 sequences (100000208 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 682702 reads in 85.794 CPU sec, 2.713 real sec
sorting block 47, #reads = 1083710 mem = 536877907 peak = 1113051136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282172, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 312, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (329.83, 155.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681846 sequences (100000216 bp) in 2.356 CPU sec, 2.482 real sec...
[M::process] read 269588 sequences (39545722 bp) in 0.740 CPU sec, 0.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281857, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 314, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (332.50, 157.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682646 reads in 131.886 CPU sec, 4.209 real sec
sorting block 48, #reads = 1083096 mem = 536878657 peak = 1113051136
[M::mem_process_seqs] Processed 681952 reads in 83.022 CPU sec, 2.647 real sec
sorting block 49, #reads = 1084188 mem = 536878600 peak = 1183236096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282057, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (336.16, 160.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 111320, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (336.99, 161.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681846 reads in 94.755 CPU sec, 3.085 real sec
[M::mem_process_seqs] Processed 269588 reads in 40.781 CPU sec, 1.320 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 54922442
overall: 69786644480 mem 7383.527 user 306.953 sys 1456.952 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.659_eZ4-N1-BDNA-1_B23KGCJLT4_1.L002\tPU:L002\tSM:659_eZ4-N1-BDNA-1_B23KGCJLT4_1\tLB:659_eZ4-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_eZ4-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 659_eZ4-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 1456.986 sec; CPU: 7690.483 sec
sorting block 50, #reads = 980315 mem = 485798412 peak = 1183236096
uniq header count 1
merging 51 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2825685429
output reads: 55178239
bam_mem_sort: 51 calls 18.930 user 0.250 sys 19.310 real
bam_write: 51 calls 37.245 user 1.072 sys 38.670 real
execute: 1 calls 10.449 user 0.764 sys 1472.620 real
merge_files: 1 calls 10.430 user 0.745 sys 14.418 real
parse_chunk: 815 calls 24.629 user 6.984 sys 32.499 real
read_chunk: 2198 calls 19.859 user 20.778 sys 197.638 real
sort_block: 1 calls 16.892 user 20.206 sys 192.699 real
write_chunk: 1381 calls 10.058 user 0.576 sys 13.684 real
overall: 2516901888 mem 551.694 user 35.641 sys 1472.640 real
ls: cannot access '*.cram': No such file or directory