cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0004_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1586.514 CPU sec, 57.085 real sec
[M::process] read 687022 sequences (100000009 bp) in 0.909 CPU sec, 0.916 real sec...
[M::process] read 685488 sequences (100000200 bp) in 1.139 CPU sec, 1.172 real sec...
[M::process] read 684548 sequences (100000160 bp) in 1.155 CPU sec, 1.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285297, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (309.51, 150.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684788 sequences (100000215 bp) in 1.109 CPU sec, 1.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285329, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (272.62, 112.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1419)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (316.80, 153.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284430, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 298, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (320.58, 154.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687022 reads in 203.952 CPU sec, 6.564 real sec
[M::mem_process_seqs] Processed 685488 reads in 130.524 CPU sec, 4.229 real sec
[M::mem_process_seqs] Processed 684548 reads in 141.509 CPU sec, 4.603 real sec
sorting block 0, #reads = 1073817 mem = 536881511 peak = 603271168
[M::process] read 683438 sequences (100000114 bp) in 1.617 CPU sec, 1.766 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284567, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (100, 181, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (180.71, 73.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 732)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 300, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (321.70, 155.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683246 sequences (100000091 bp) in 1.207 CPU sec, 1.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283659, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (324.50, 156.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684788 reads in 124.536 CPU sec, 3.965 real sec
[M::process] read 682950 sequences (100000144 bp) in 1.050 CPU sec, 1.049 real sec...
sorting block 1, #reads = 1072494 mem = 536878881 peak = 854929408
[M::mem_process_seqs] Processed 683438 reads in 78.528 CPU sec, 2.483 real sec
sorting block 2, #reads = 1072333 mem = 536880579 peak = 873701376
[M::process] read 686050 sequences (100000003 bp) in 1.423 CPU sec, 1.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284604, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (324.35, 155.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686254 sequences (100000279 bp) in 1.171 CPU sec, 1.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284296, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (327.62, 157.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284868, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (315.05, 153.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 247, 690)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828)
[M::mem_pestat] mean and std.dev: (369.50, 304.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2397)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683246 reads in 176.545 CPU sec, 5.669 real sec
[M::mem_process_seqs] Processed 682950 reads in 130.415 CPU sec, 4.202 real sec
[M::mem_process_seqs] Processed 686050 reads in 141.565 CPU sec, 4.580 real sec
sorting block 3, #reads = 1071907 mem = 536878327 peak = 873701376
[M::process] read 685362 sequences (100000269 bp) in 1.786 CPU sec, 1.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284865, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (315.45, 153.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1072997 mem = 536883188 peak = 905678848
[M::process] read 684698 sequences (100000228 bp) in 1.360 CPU sec, 1.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285013, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 298, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (320.81, 156.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686254 reads in 124.504 CPU sec, 3.988 real sec
[M::mem_process_seqs] Processed 685362 reads in 78.342 CPU sec, 2.483 real sec
[M::process] read 683242 sequences (100000048 bp) in 1.475 CPU sec, 1.544 real sec...
sorting block 5, #reads = 1074874 mem = 536880716 peak = 905678848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284963, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 175, 233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 355)
[M::mem_pestat] mean and std.dev: (181.92, 33.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 416)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 301, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (323.81, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683584 sequences (100000178 bp) in 1.527 CPU sec, 1.626 real sec...
[M::process] read 683586 sequences (100000226 bp) in 1.160 CPU sec, 1.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284748, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (328.18, 157.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 173, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 296)
[M::mem_pestat] mean and std.dev: (170.70, 37.76)
[M::mem_pestat] low and high boundaries for proper pairs: (2, 345)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684698 reads in 125.177 CPU sec, 4.001 real sec
[M::mem_process_seqs] Processed 683242 reads in 78.729 CPU sec, 2.509 real sec
sorting block 6, #reads = 1074356 mem = 536880760 peak = 905678848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284058, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (324.72, 157.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685740 sequences (100000142 bp) in 1.664 CPU sec, 1.788 real sec...
[M::process] read 685942 sequences (100000124 bp) in 1.210 CPU sec, 1.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284358, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 304, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (326.70, 158.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (252.00, 215.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683584 reads in 125.126 CPU sec, 4.003 real sec
[M::mem_process_seqs] Processed 683586 reads in 78.151 CPU sec, 2.495 real sec
sorting block 7, #reads = 1071893 mem = 536885354 peak = 905678848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284986, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (317.04, 154.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685068 sequences (100000265 bp) in 1.811 CPU sec, 1.922 real sec...
[M::process] read 684300 sequences (100000100 bp) in 1.220 CPU sec, 1.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285198, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 295, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (315.17, 151.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685740 reads in 125.193 CPU sec, 3.998 real sec
sorting block 8, #reads = 1071677 mem = 536881784 peak = 905678848
[M::mem_process_seqs] Processed 685942 reads in 78.311 CPU sec, 2.499 real sec
sorting block 9, #reads = 1073599 mem = 536879229 peak = 905678848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285180, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 301, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (321.78, 155.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682892 sequences (100000219 bp) in 1.659 CPU sec, 1.795 real sec...
[M::process] read 682826 sequences (100000027 bp) in 1.250 CPU sec, 1.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284556, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 248, 846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2228)
[M::mem_pestat] mean and std.dev: (321.20, 267.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2919)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 304, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (325.75, 157.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 685068 reads in 125.287 CPU sec, 4.011 real sec
[M::mem_process_seqs] Processed 684300 reads in 78.655 CPU sec, 2.506 real sec
sorting block 10, #reads = 1074288 mem = 536877863 peak = 905678848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284033, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (331.87, 158.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 192, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (214.11, 96.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RR
[M::process] read 682952 sequences (100000263 bp) in 1.942 CPU sec, 2.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283988, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (329.94, 158.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684438 sequences (100000167 bp) in 1.270 CPU sec, 1.261 real sec...
[M::mem_process_seqs] Processed 682892 reads in 126.866 CPU sec, 4.054 real sec
[M::mem_process_seqs] Processed 682826 reads in 79.504 CPU sec, 2.531 real sec
sorting block 11, #reads = 1073377 mem = 536879553 peak = 905678848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284298, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 306, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (328.31, 158.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685842 sequences (100000225 bp) in 1.739 CPU sec, 1.873 real sec...
[M::process] read 685178 sequences (100000102 bp) in 1.250 CPU sec, 1.253 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284507, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 300, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (321.77, 155.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682952 reads in 126.305 CPU sec, 4.040 real sec
sorting block 12, #reads = 1071791 mem = 536877628 peak = 905678848
[M::mem_process_seqs] Processed 684438 reads in 79.105 CPU sec, 2.519 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285028, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (312.31, 149.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1072340 mem = 536880994 peak = 905678848
[M::process] read 684738 sequences (100000189 bp) in 2.014 CPU sec, 2.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284695, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 298, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (316.47, 150.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683594 sequences (100000190 bp) in 1.290 CPU sec, 1.286 real sec...
[M::mem_process_seqs] Processed 685842 reads in 125.311 CPU sec, 4.003 real sec
[M::mem_process_seqs] Processed 685178 reads in 79.193 CPU sec, 2.525 real sec
sorting block 14, #reads = 1074642 mem = 536880345 peak = 905678848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284038, 0, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 167, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358)
[M::mem_pestat] mean and std.dev: (163.67, 64.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 434)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 302, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (323.17, 156.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 196, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402)
[M::mem_pestat] mean and std.dev: (193.90, 80.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 515)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682812 sequences (100000072 bp) in 1.880 CPU sec, 2.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284377, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 308, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (328.44, 157.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682724 sequences (100000020 bp) in 1.290 CPU sec, 1.287 real sec...
[M::mem_process_seqs] Processed 684738 reads in 127.389 CPU sec, 4.083 real sec
[M::mem_process_seqs] Processed 683594 reads in 80.447 CPU sec, 2.560 real sec
sorting block 15, #reads = 1073425 mem = 536883127 peak = 905678848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283496, 1, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 312, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (331.53, 158.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 379, 848)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2156)
[M::mem_pestat] mean and std.dev: (399.58, 340.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2810)
[M::mem_pestat] skip orientation RR
[M::process] read 683166 sequences (100000296 bp) in 2.084 CPU sec, 2.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284032, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 309, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (330.59, 158.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688504 sequences (100000220 bp) in 1.349 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 682812 reads in 127.863 CPU sec, 4.082 real sec
[M::mem_process_seqs] Processed 682724 reads in 79.979 CPU sec, 2.544 real sec
sorting block 16, #reads = 1072444 mem = 536880036 peak = 905678848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284359, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 150, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336)
[M::mem_pestat] mean and std.dev: (162.40, 47.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 405)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (327.25, 158.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686704 sequences (100000092 bp) in 1.845 CPU sec, 1.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286466, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (305.80, 150.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685946 sequences (100000212 bp) in 1.340 CPU sec, 1.334 real sec...
[M::mem_process_seqs] Processed 683166 reads in 130.878 CPU sec, 4.181 real sec
sorting block 17, #reads = 1071666 mem = 536879385 peak = 905678848
[M::mem_process_seqs] Processed 688504 reads in 83.651 CPU sec, 2.659 real sec
sorting block 18, #reads = 1071965 mem = 536879204 peak = 975450112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286235, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 166, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445)
[M::mem_pestat] mean and std.dev: (166.50, 61.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 546)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (313.18, 153.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 205, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (203.22, 76.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685982 sequences (100000227 bp) in 2.236 CPU sec, 2.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285672, 0, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 202, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416)
[M::mem_pestat] mean and std.dev: (185.75, 65.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 509)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (316.84, 154.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684184 sequences (100000042 bp) in 1.334 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 686704 reads in 132.509 CPU sec, 4.220 real sec
[M::mem_process_seqs] Processed 685946 reads in 82.622 CPU sec, 2.619 real sec
sorting block 19, #reads = 1072681 mem = 536877282 peak = 975450112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285373, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (315.24, 153.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 179, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (217.54, 96.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 707)
[M::mem_pestat] skip orientation RR
[M::process] read 683476 sequences (100000253 bp) in 1.894 CPU sec, 2.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284553, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 300, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (320.99, 153.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684070 sequences (100000068 bp) in 1.330 CPU sec, 1.337 real sec...
[M::mem_process_seqs] Processed 685982 reads in 133.651 CPU sec, 4.267 real sec
[M::mem_process_seqs] Processed 684184 reads in 83.826 CPU sec, 2.659 real sec
sorting block 20, #reads = 1071559 mem = 536879725 peak = 975450112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284183, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 184, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (40, 345)
[M::mem_pestat] mean and std.dev: (189.54, 57.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 421)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (321.14, 152.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 689686 sequences (100000028 bp) in 2.038 CPU sec, 2.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283905, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 297, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (317.67, 151.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687634 sequences (100000216 bp) in 1.320 CPU sec, 1.315 real sec...
[M::mem_process_seqs] Processed 683476 reads in 134.717 CPU sec, 4.296 real sec
sorting block 21, #reads = 1070412 mem = 536881662 peak = 975450112
[M::mem_process_seqs] Processed 684070 reads in 85.145 CPU sec, 2.700 real sec
sorting block 22, #reads = 1067247 mem = 536880545 peak = 975450112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287313, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 277, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (300.62, 148.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686854 sequences (100000116 bp) in 1.862 CPU sec, 1.956 real sec...
[M::process] read 685726 sequences (100000035 bp) in 1.320 CPU sec, 1.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286195, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 287, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (308.00, 149.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689686 reads in 131.413 CPU sec, 4.185 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286200, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 189, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (164.64, 40.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (311.01, 150.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684028 sequences (100000033 bp) in 1.727 CPU sec, 1.728 real sec...
[M::mem_process_seqs] Processed 686854 reads in 70.764 CPU sec, 2.239 real sec
sorting block 23, #reads = 1072713 mem = 536880145 peak = 975450112
[M::mem_process_seqs] Processed 687634 reads in 181.749 CPU sec, 5.775 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285856, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (315.24, 151.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1072156 mem = 536879145 peak = 975450112
[M::process] read 683434 sequences (100000075 bp) in 1.929 CPU sec, 2.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285112, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (304, 323, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (210, 445)
[M::mem_pestat] mean and std.dev: (325.78, 34.16)
[M::mem_pestat] low and high boundaries for proper pairs: (163, 492)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (322.39, 154.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 685726 reads in 139.703 CPU sec, 4.439 real sec
[M::mem_process_seqs] Processed 684028 reads in 81.477 CPU sec, 2.572 real sec
[M::process] read 686236 sequences (100000233 bp) in 1.598 CPU sec, 1.646 real sec...
sorting block 25, #reads = 1071854 mem = 536878371 peak = 975450112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284520, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 217, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500)
[M::mem_pestat] mean and std.dev: (200.00, 67.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 600)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (323.17, 154.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687402 sequences (100000223 bp) in 2.020 CPU sec, 2.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284915, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (312.68, 151.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686230 sequences (100000070 bp) in 1.280 CPU sec, 1.279 real sec...
[M::mem_process_seqs] Processed 683434 reads in 132.463 CPU sec, 4.213 real sec
[M::process] read 685870 sequences (100000030 bp) in 1.414 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286168, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (305.94, 147.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687402 reads in 74.190 CPU sec, 2.567 real sec
sorting block 26, #reads = 1070498 mem = 536880189 peak = 975450112
[M::mem_process_seqs] Processed 686236 reads in 180.133 CPU sec, 5.937 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285323, 0, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (309.50, 147.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 204, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (185.33, 86.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RR
sorting block 27, #reads = 1069425 mem = 536878162 peak = 1040044032
[M::process] read 685044 sequences (100000169 bp) in 2.053 CPU sec, 2.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285716, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 293, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (312.78, 149.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686230 reads in 138.490 CPU sec, 4.405 real sec
[M::mem_process_seqs] Processed 685870 reads in 80.441 CPU sec, 2.539 real sec
[M::process] read 684318 sequences (100000106 bp) in 1.508 CPU sec, 1.551 real sec...
sorting block 28, #reads = 1073137 mem = 536879196 peak = 1040044032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285397, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (314.60, 148.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684342 sequences (100000084 bp) in 1.982 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285222, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 178, 849)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2255)
[M::mem_pestat] mean and std.dev: (265.62, 230.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2958)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (317.45, 149.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687018 sequences (100000052 bp) in 1.280 CPU sec, 1.277 real sec...
[M::mem_process_seqs] Processed 685044 reads in 130.106 CPU sec, 4.138 real sec
[M::mem_process_seqs] Processed 684318 reads in 81.988 CPU sec, 2.598 real sec
sorting block 29, #reads = 1072384 mem = 536879127 peak = 1040044032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285085, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 224, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 460)
[M::mem_pestat] mean and std.dev: (215.80, 75.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 549)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (320.52, 152.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 168, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (196.89, 76.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 745)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688246 sequences (100000074 bp) in 2.224 CPU sec, 2.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285596, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 286, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (309.46, 151.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687146 sequences (100000143 bp) in 1.310 CPU sec, 1.319 real sec...
[M::mem_process_seqs] Processed 684342 reads in 133.778 CPU sec, 4.248 real sec
sorting block 30, #reads = 1070407 mem = 536880269 peak = 1040044032
[M::mem_process_seqs] Processed 687018 reads in 85.996 CPU sec, 2.723 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286489, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (305.84, 148.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1068691 mem = 536879745 peak = 1040044032
[M::process] read 686038 sequences (100000224 bp) in 2.192 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286190, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (310.27, 149.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685610 sequences (100000085 bp) in 1.290 CPU sec, 1.292 real sec...
[M::mem_process_seqs] Processed 688246 reads in 131.638 CPU sec, 4.184 real sec
[M::mem_process_seqs] Processed 687146 reads in 82.390 CPU sec, 2.609 real sec
sorting block 32, #reads = 1071462 mem = 536882837 peak = 1040044032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285148, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (314.95, 151.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 267, 1233)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3407)
[M::mem_pestat] mean and std.dev: (532.00, 469.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4494)
[M::mem_pestat] skip orientation RR
[M::process] read 684778 sequences (100000281 bp) in 2.091 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285102, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 139, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (193.27, 108.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::process] read 683904 sequences (100000006 bp) in 1.320 CPU sec, 1.315 real sec...
[M::mem_pestat] mean and std.dev: (315.15, 150.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 200, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (200.11, 69.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 747)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686038 reads in 130.994 CPU sec, 4.162 real sec
[M::mem_process_seqs] Processed 685610 reads in 82.594 CPU sec, 2.616 real sec
sorting block 33, #reads = 1070895 mem = 536879927 peak = 1040044032
[M::process] read 224600 sequences (32850663 bp) in 0.964 CPU sec, 0.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285059, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 297, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (315.32, 148.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 213, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (237.38, 80.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284528, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 246, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477)
[M::mem_pestat] mean and std.dev: (200.08, 64.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 586)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 299, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (318.29, 150.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 211, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (237.85, 83.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684778 reads in 126.660 CPU sec, 4.099 real sec
[M::mem_process_seqs] Processed 683904 reads in 79.155 CPU sec, 2.564 real sec
sorting block 34, #reads = 1070400 mem = 536878417 peak = 1040044032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 93312, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (318.67, 151.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 224600 reads in 24.720 CPU sec, 0.838 real sec
sorting block 35, #reads = 1069911 mem = 536878805 peak = 1137373184
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 38589478
overall: 69706190848 mem 5276.076 user 269.443 sys 191.531 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0004_B23KGCJLT4_1.L008\tPU:L008\tSM:NA12878_c_0002_gDNA_0004_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0004_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0004_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0004_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 191.575 sec; CPU: 5545.522 sec
sorting block 36, #reads = 184574 mem = 92934215 peak = 1137373184
uniq header count 1
merging 37 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2017187930
output reads: 38776291
bam_mem_sort: 37 calls 13.201 user 0.269 sys 13.553 real
bam_write: 37 calls 22.609 user 0.533 sys 23.363 real
execute: 1 calls 7.299 user 0.675 sys 202.230 real
merge_files: 1 calls 7.289 user 0.646 sys 10.408 real
parse_chunk: 579 calls 17.630 user 5.016 sys 23.020 real
read_chunk: 1565 calls 14.305 user 12.703 sys 131.490 real
sort_block: 1 calls 11.335 user 12.221 sys 127.222 real
write_chunk: 984 calls 6.898 user 0.441 sys 9.667 real
overall: 2324656128 mem 390.511 user 23.983 sys 202.252 real
ls: cannot access '*.cram': No such file or directory