cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_0I-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
Received only 12 licenses out of 31 requested for sentieon:klib
Received only 12 licenses out of 31 requested for sentieon:klib
Received only 12 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 1717.688 CPU sec, 748.314 real sec
[M::process] read 683528 sequences (100000100 bp) in 0.918 CPU sec, 0.925 real sec...
[M::process] read 683556 sequences (100000044 bp) in 1.060 CPU sec, 1.057 real sec...
[M::process] read 683156 sequences (100000158 bp) in 1.059 CPU sec, 1.062 real sec...
[M::process] read 682752 sequences (100000212 bp) in 1.070 CPU sec, 1.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282797, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 315, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (334.24, 160.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282787, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (338.46, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282909, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (335.72, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683528 reads in 184.858 CPU sec, 15.316 real sec
[M::mem_process_seqs] Processed 683156 reads in 133.148 CPU sec, 11.256 real sec
sorting block 0, #reads = 1084868 mem = 536879934 peak = 589037568
[M::mem_process_seqs] Processed 683556 reads in 146.210 CPU sec, 12.307 real sec
[M::process] read 682336 sequences (100000125 bp) in 1.447 CPU sec, 1.520 real sec...
[M::process] read 682700 sequences (100000222 bp) in 1.445 CPU sec, 1.511 real sec...
[M::process] read 682232 sequences (100000287 bp) in 1.050 CPU sec, 1.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282371, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (339.68, 163.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282222, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (340.45, 164.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
Received only 12 licenses out of 31 requested for sentieon:klib
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282234, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 314, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (336.40, 163.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682752 reads in 182.871 CPU sec, 15.093 real sec
sorting block 1, #reads = 1085478 mem = 536879477 peak = 882569216
[M::mem_process_seqs] Processed 682336 reads in 136.068 CPU sec, 11.363 real sec
[M::mem_process_seqs] Processed 682700 reads in 149.529 CPU sec, 12.445 real sec
sorting block 2, #reads = 1084274 mem = 536878918 peak = 882569216
[M::process] read 683290 sequences (100000081 bp) in 1.625 CPU sec, 1.746 real sec...
[M::process] read 683694 sequences (100000090 bp) in 1.618 CPU sec, 1.729 real sec...
[M::process] read 683510 sequences (100000177 bp) in 1.143 CPU sec, 1.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282333, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 318, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (340.42, 165.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282654, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (336.94, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282697, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (337.48, 161.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682232 reads in 187.291 CPU sec, 15.468 real sec
sorting block 3, #reads = 1083973 mem = 536878061 peak = 882569216
[M::mem_process_seqs] Processed 683694 reads in 137.836 CPU sec, 11.536 real sec
[M::mem_process_seqs] Processed 683290 reads in 151.089 CPU sec, 12.604 real sec
sorting block 4, #reads = 1083849 mem = 536879417 peak = 882569216
[M::process] read 683202 sequences (100000145 bp) in 1.755 CPU sec, 1.881 real sec...
[M::process] read 682828 sequences (100000164 bp) in 1.687 CPU sec, 1.796 real sec...
[M::process] read 682934 sequences (100000158 bp) in 1.300 CPU sec, 1.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282815, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 319, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (338.55, 163.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282783, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 321, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (340.73, 164.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282550, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 319, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (340.42, 164.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683510 reads in 185.774 CPU sec, 15.294 real sec
sorting block 5, #reads = 1085429 mem = 536877607 peak = 882569216
[M::mem_process_seqs] Processed 683202 reads in 135.038 CPU sec, 11.288 real sec
[M::mem_process_seqs] Processed 682828 reads in 148.675 CPU sec, 12.391 real sec
sorting block 6, #reads = 1085408 mem = 536888484 peak = 882569216
[M::process] read 682648 sequences (100000093 bp) in 1.987 CPU sec, 2.138 real sec...
[M::process] read 683382 sequences (100000046 bp) in 1.715 CPU sec, 1.807 real sec...
[M::process] read 683950 sequences (100000116 bp) in 1.250 CPU sec, 1.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282666, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (336.83, 162.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683382 reads in 67.162 CPU sec, 5.605 real sec
sorting block 7, #reads = 1084439 mem = 536878588 peak = 882569216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282938, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 315, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (337.64, 164.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683806 sequences (100000067 bp) in 1.388 CPU sec, 1.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283009, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 317, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (339.50, 165.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682934 reads in 196.850 CPU sec, 16.124 real sec
[M::mem_process_seqs] Processed 682648 reads in 147.578 CPU sec, 12.242 real sec
sorting block 8, #reads = 1083918 mem = 536878353 peak = 882569216
[M::process] read 683024 sequences (100000157 bp) in 2.108 CPU sec, 2.270 real sec...
[M::process] read 682652 sequences (100000088 bp) in 1.320 CPU sec, 1.319 real sec...
Received only 12 licenses out of 31 requested for sentieon:klib
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282861, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (335.21, 160.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283028, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (336.53, 162.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282685, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 320, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (339.80, 164.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683950 reads in 178.418 CPU sec, 14.672 real sec
sorting block 9, #reads = 1085306 mem = 536877952 peak = 882569216
[M::mem_process_seqs] Processed 683806 reads in 131.866 CPU sec, 11.002 real sec
[M::mem_process_seqs] Processed 683024 reads in 145.197 CPU sec, 12.083 real sec
[M::process] read 682078 sequences (100000041 bp) in 2.469 CPU sec, 2.664 real sec...
sorting block 10, #reads = 1084493 mem = 536878433 peak = 882569216
[M::process] read 682226 sequences (100000093 bp) in 1.949 CPU sec, 2.088 real sec...
[M::process] read 683370 sequences (100000041 bp) in 1.290 CPU sec, 1.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282615, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (342.79, 166.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682226 reads in 65.619 CPU sec, 5.477 real sec
sorting block 11, #reads = 1084535 mem = 536881473 peak = 882569216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282678, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 323, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (343.45, 166.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684172 sequences (100000144 bp) in 1.445 CPU sec, 1.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282347, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 322, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (343.18, 165.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682652 reads in 196.344 CPU sec, 16.100 real sec
[M::mem_process_seqs] Processed 682078 reads in 147.156 CPU sec, 12.197 real sec
sorting block 12, #reads = 1084609 mem = 536877199 peak = 882569216
[M::process] read 684288 sequences (100000125 bp) in 2.232 CPU sec, 2.429 real sec...
[M::process] read 683620 sequences (100000062 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282677, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (337.66, 164.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282840, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 314, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (332.64, 160.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683370 reads in 125.485 CPU sec, 10.252 real sec
[M::mem_process_seqs] Processed 684172 reads in 137.952 CPU sec, 11.327 real sec
sorting block 13, #reads = 1084629 mem = 536877703 peak = 882569216
[M::process] read 683036 sequences (100000225 bp) in 2.068 CPU sec, 2.239 real sec...
[M::process] read 681970 sequences (100000244 bp) in 1.370 CPU sec, 1.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282810, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 312, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (332.07, 161.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283067, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (335.70, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282333, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (337.46, 163.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684288 reads in 185.726 CPU sec, 15.285 real sec
sorting block 14, #reads = 1086074 mem = 536878562 peak = 882569216
[M::mem_process_seqs] Processed 683620 reads in 138.154 CPU sec, 11.544 real sec
[M::process] read 681526 sequences (100000135 bp) in 2.375 CPU sec, 2.520 real sec...
[M::process] read 683000 sequences (100000092 bp) in 1.500 CPU sec, 1.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282205, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 322, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (342.07, 164.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681970 reads in 75.581 CPU sec, 3.134 real sec
sorting block 15, #reads = 1085394 mem = 536877675 peak = 882569216
[M::mem_process_seqs] Processed 683036 reads in 254.413 CPU sec, 16.667 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282255, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (343.94, 164.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1083344 mem = 536876712 peak = 882569216
[M::process] read 685420 sequences (100000133 bp) in 2.195 CPU sec, 2.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282398, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (337.70, 164.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681526 reads in 147.723 CPU sec, 4.724 real sec
[M::mem_process_seqs] Processed 683000 reads in 88.126 CPU sec, 2.802 real sec
[M::process] read 684898 sequences (100000167 bp) in 1.897 CPU sec, 1.992 real sec...
sorting block 17, #reads = 1083310 mem = 536876913 peak = 882569216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283750, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 307, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (329.46, 161.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684526 sequences (100000146 bp) in 2.202 CPU sec, 2.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283215, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 308, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (328.40, 159.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685420 reads in 138.719 CPU sec, 4.423 real sec
[M::mem_process_seqs] Processed 684898 reads in 87.040 CPU sec, 2.752 real sec
[M::process] read 683920 sequences (100000114 bp) in 1.709 CPU sec, 1.741 real sec...
sorting block 18, #reads = 1084048 mem = 536878416 peak = 882569216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283928, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 308, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (327.93, 157.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683558 sequences (100000112 bp) in 2.473 CPU sec, 2.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283270, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 311, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (330.35, 158.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684526 reads in 138.134 CPU sec, 4.422 real sec
[M::mem_process_seqs] Processed 683920 reads in 88.139 CPU sec, 2.789 real sec
sorting block 19, #reads = 1084549 mem = 536878898 peak = 882569216
[M::process] read 683412 sequences (100000144 bp) in 2.133 CPU sec, 2.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283047, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 309, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (328.94, 157.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683392 sequences (100000221 bp) in 2.143 CPU sec, 2.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282761, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 309, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (328.51, 156.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683558 reads in 142.752 CPU sec, 4.544 real sec
sorting block 20, #reads = 1083467 mem = 536879549 peak = 882569216
[M::mem_process_seqs] Processed 683412 reads in 90.406 CPU sec, 2.852 real sec
[M::process] read 684930 sequences (100000295 bp) in 1.634 CPU sec, 1.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282787, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 311, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (331.93, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1082763 mem = 536880273 peak = 962551808
[M::process] read 684800 sequences (100000097 bp) in 2.136 CPU sec, 2.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283871, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (327.09, 156.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684906 sequences (100000261 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 683392 reads in 142.570 CPU sec, 4.544 real sec
[M::mem_process_seqs] Processed 684930 reads in 88.035 CPU sec, 2.789 real sec
sorting block 22, #reads = 1081889 mem = 536884931 peak = 962551808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283335, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 306, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (324.01, 154.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684146 sequences (100000034 bp) in 2.120 CPU sec, 2.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283790, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 306, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (324.32, 154.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683378 sequences (100000132 bp) in 1.490 CPU sec, 1.488 real sec...
[M::mem_process_seqs] Processed 684800 reads in 139.171 CPU sec, 4.433 real sec
[M::mem_process_seqs] Processed 684906 reads in 88.001 CPU sec, 2.783 real sec
sorting block 23, #reads = 1084766 mem = 536879744 peak = 962551808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283599, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 309, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (327.52, 156.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683056 sequences (100000060 bp) in 2.151 CPU sec, 2.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283133, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 313, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (331.03, 157.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684146 reads in 135.197 CPU sec, 4.303 real sec
[M::process] read 682848 sequences (100000030 bp) in 1.595 CPU sec, 1.610 real sec...
[M::mem_process_seqs] Processed 683378 reads in 85.959 CPU sec, 2.712 real sec
sorting block 24, #reads = 1083980 mem = 536878191 peak = 962551808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282619, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (333.36, 159.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685126 sequences (100000301 bp) in 2.347 CPU sec, 2.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282403, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (335.48, 161.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684866 sequences (100000027 bp) in 1.470 CPU sec, 1.474 real sec...
[M::mem_process_seqs] Processed 683056 reads in 138.840 CPU sec, 4.411 real sec
sorting block 25, #reads = 1083555 mem = 536877342 peak = 962551808
[M::mem_process_seqs] Processed 682848 reads in 90.176 CPU sec, 2.853 real sec
sorting block 26, #reads = 1081771 mem = 536885236 peak = 1020284928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284022, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 308, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (328.73, 160.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684140 sequences (100000084 bp) in 1.942 CPU sec, 2.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284024, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 305, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (324.81, 155.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683980 sequences (100000257 bp) in 1.340 CPU sec, 1.344 real sec...
[M::mem_process_seqs] Processed 685126 reads in 134.518 CPU sec, 4.285 real sec
[M::mem_process_seqs] Processed 684866 reads in 84.301 CPU sec, 2.672 real sec
sorting block 27, #reads = 1082701 mem = 536877521 peak = 1020284928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283779, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 308, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (325.96, 154.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683086 sequences (100000125 bp) in 2.228 CPU sec, 2.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283670, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 311, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (329.10, 156.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682904 sequences (100000126 bp) in 1.360 CPU sec, 1.364 real sec...
[M::mem_process_seqs] Processed 684140 reads in 134.870 CPU sec, 4.287 real sec
[M::mem_process_seqs] Processed 683980 reads in 87.730 CPU sec, 2.779 real sec
sorting block 28, #reads = 1085137 mem = 536880431 peak = 1020284928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283420, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (332.39, 157.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682930 sequences (100000189 bp) in 1.942 CPU sec, 2.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283214, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (333.62, 158.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685380 sequences (100000100 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 683086 reads in 135.879 CPU sec, 4.325 real sec
sorting block 29, #reads = 1082241 mem = 536879851 peak = 1020284928
[M::mem_process_seqs] Processed 682904 reads in 85.079 CPU sec, 2.699 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282879, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (336.12, 161.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 30, #reads = 1083351 mem = 536878466 peak = 1020284928
[M::process] read 685620 sequences (100000042 bp) in 2.182 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284009, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 307, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (329.92, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685298 sequences (100000130 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_process_seqs] Processed 682930 reads in 138.050 CPU sec, 4.388 real sec
[M::mem_process_seqs] Processed 685380 reads in 87.690 CPU sec, 2.781 real sec
sorting block 31, #reads = 1081991 mem = 536879398 peak = 1020284928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284035, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 304, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (325.58, 159.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683950 sequences (100000195 bp) in 1.948 CPU sec, 2.054 real sec...
[M::process] read 682944 sequences (100000238 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283949, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 306, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (328.41, 160.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685620 reads in 139.675 CPU sec, 4.450 real sec
[M::mem_process_seqs] Processed 685298 reads in 88.867 CPU sec, 2.820 real sec
sorting block 32, #reads = 1083127 mem = 536879999 peak = 1020284928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283254, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 310, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (330.64, 159.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682230 sequences (100000173 bp) in 2.012 CPU sec, 2.114 real sec...
[M::process] read 682088 sequences (100000017 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283391, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 314, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (331.95, 156.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683950 reads in 145.076 CPU sec, 4.621 real sec
[M::mem_process_seqs] Processed 682944 reads in 86.177 CPU sec, 2.736 real sec
sorting block 33, #reads = 1079755 mem = 536878317 peak = 1020284928
[M::process] read 123142 sequences (18046179 bp) in 0.597 CPU sec, 0.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282199, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 316, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (333.41, 156.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282897, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 319, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (337.24, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 50977, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 318, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (337.33, 160.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682230 reads in 140.987 CPU sec, 4.572 real sec
sorting block 34, #reads = 1082837 mem = 536881188 peak = 1020284928
[M::mem_process_seqs] Processed 682088 reads in 93.033 CPU sec, 3.010 real sec
[M::mem_process_seqs] Processed 123142 reads in 95.431 CPU sec, 3.094 real sec
sorting block 35, #reads = 1082590 mem = 536881464 peak = 1070673920
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 39083340
overall: 70315487232 mem 5581.414 user 306.367 sys 982.254 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.1029_0I-N1-BDNA-1_B23KGCJLT4_1.L006\tPU:L006\tSM:1029_0I-N1-BDNA-1_B23KGCJLT4_1\tLB:1029_0I-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_0I-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 1029_0I-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 982.291 sec; CPU: 5887.784 sec
sorting block 36, #reads = 213719 mem = 106300722 peak = 1070673920
uniq header count 1
merging 37 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2097142754
output reads: 39231567
bam_mem_sort: 37 calls 14.648 user 0.170 sys 14.912 real
bam_write: 37 calls 16.476 user 0.526 sys 17.097 real
execute: 1 calls 7.693 user 0.408 sys 993.234 real
merge_files: 1 calls 7.677 user 0.384 sys 10.748 real
parse_chunk: 580 calls 19.026 user 5.119 sys 24.592 real
read_chunk: 1564 calls 13.495 user 14.408 sys 221.549 real
sort_block: 1 calls 11.638 user 13.925 sys 217.911 real
write_chunk: 982 calls 7.302 user 0.228 sys 10.039 real
overall: 2354794496 mem 412.768 user 24.106 sys 993.261 real
ls: cannot access '*.cram': No such file or directory