Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/d0bdba507c581bc7f592dcaefc7898/NTC_0002_0014_B23KGCJLT4_1/NTC_0002_0014_B23KGCJLT4_1_R1.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/22/bfa3965123dce1b6c3728f2da1b748/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ce/d0bdba507c581bc7f592dcaefc7898/NTC_0002_0014_B23KGCJLT4_1/NTC_0002_0014_B23KGCJLT4_1_R2.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/22/bfa3965123dce1b6c3728f2da1b748/.command.run ==> STAGING COMPLETE (7 inputs) cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NTC_0002_0014_B23KGCJLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. No more license available for sentieon:klib version: sentieon-genomics-202308.03 No more license available for sentieon:klib [M::bwa_idx_load_from_disk] read 3171 ALT contigs No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib [M::main_mem] bwa index loaded in 1633.791 CPU sec, 473.640 real sec [M::process] read 1122 sequences (169416 bp) in 0.000 CPU sec, 0.001 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 1, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] skip orientation FR as there are not enough pairs [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 1122 reads in 2.253 CPU sec, 0.078 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 1122 overall: 68496678912 mem 1518.898 user 122.990 sys 479.574 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NTC_0002_0014_B23KGCJLT4_1.L004\tPU:L004\tSM:NTC_0002_0014_B23KGCJLT4_1\tLB:NTC_0002_0014_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NTC_0002_0014_B23KGCJLT4_1_R1.fastq.gz NTC_0002_0014_B23KGCJLT4_1_R2.fastq.gz [main] Real time: 479.610 sec; CPU: 1641.891 sec sorting block 0, #reads = 1144 mem = 636672 peak = 78757888 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 112181 output reads: 1144 bam_mem_sort: 1 calls 0.000 user 0.000 sys 0.000 real bam_write: 1 calls 0.003 user 0.000 sys 0.003 real execute: 1 calls 0.035 user 0.108 sys 479.695 real merge_files: 1 calls 0.027 user 0.069 sys 0.105 real parse_chunk: 1 calls 0.000 user 0.000 sys 0.001 real read_chunk: 4 calls 0.001 user 0.000 sys 5.847 real sort_block: 1 calls 0.004 user 0.000 sys 5.847 real write_chunk: 1 calls 0.000 user 0.000 sys 0.000 real overall: 265994240 mem 0.063 user 0.140 sys 479.717 real ls: cannot access '*.cram': No such file or directory