Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/48/8bb92226bffb157f04d558899ca0cb/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fc/9fe5f0ffa5c5f9872e9058cffc0288/NA12878_c_0002_gDNA_0002_B23KGCJLT4_1/NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/48/8bb92226bffb157f04d558899ca0cb/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fc/9fe5f0ffa5c5f9872e9058cffc0288/NA12878_c_0002_gDNA_0002_B23KGCJLT4_1/NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0002_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1711.257 CPU sec, 420.906 real sec
[M::process] read 690876 sequences (100000262 bp) in 0.916 CPU sec, 0.919 real sec...
[M::process] read 689566 sequences (100000290 bp) in 1.036 CPU sec, 1.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286909, 0, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 275, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (294.11, 141.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 169, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432)
[M::mem_pestat] mean and std.dev: (202.06, 89.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] skip orientation RR
[M::process] read 688860 sequences (100000145 bp) in 1.066 CPU sec, 1.083 real sec...
[M::process] read 688236 sequences (100000044 bp) in 1.059 CPU sec, 1.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286310, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (299.97, 143.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690876 reads in 127.387 CPU sec, 4.077 real sec
[M::mem_process_seqs] Processed 689566 reads in 75.086 CPU sec, 2.447 real sec
sorting block 0, #reads = 1076390 mem = 536882893 peak = 604049408
[M::process] read 687682 sequences (100000041 bp) in 1.474 CPU sec, 1.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285861, 0, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 219, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (253.40, 114.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (302.99, 145.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 226, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424)
[M::mem_pestat] mean and std.dev: (181.80, 78.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 517)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687268 sequences (100000124 bp) in 1.080 CPU sec, 1.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285583, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 177, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 284)
[M::mem_pestat] mean and std.dev: (172.56, 35.83)
[M::mem_pestat] low and high boundaries for proper pairs: (14, 329)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 286, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.41, 146.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285800, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (309.35, 148.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688860 reads in 171.738 CPU sec, 5.519 real sec
[M::mem_process_seqs] Processed 688236 reads in 127.461 CPU sec, 4.124 real sec
[M::mem_process_seqs] Processed 687682 reads in 137.952 CPU sec, 4.490 real sec
sorting block 1, #reads = 1075007 mem = 536881516 peak = 728956928
[M::process] read 686546 sequences (100000246 bp) in 1.623 CPU sec, 1.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285199, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (312.06, 150.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1074262 mem = 536880211 peak = 851144704
[M::process] read 685840 sequences (100000172 bp) in 1.334 CPU sec, 1.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284599, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (314.49, 150.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 201, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (201.75, 74.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 772)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687268 reads in 124.604 CPU sec, 3.961 real sec
[M::mem_process_seqs] Processed 686546 reads in 78.713 CPU sec, 2.477 real sec
[M::process] read 685640 sequences (100000093 bp) in 1.379 CPU sec, 1.441 real sec...
sorting block 3, #reads = 1073040 mem = 536880040 peak = 851144704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284588, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 224, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478)
[M::mem_pestat] mean and std.dev: (323.30, 203.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1914)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (315.70, 150.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685812 sequences (100000192 bp) in 1.596 CPU sec, 1.693 real sec...
[M::process] read 689862 sequences (100000110 bp) in 1.180 CPU sec, 1.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283961, 1, 19)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 295, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (315.41, 150.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (101, 179, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (220.89, 131.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685840 reads in 122.940 CPU sec, 3.911 real sec
[M::mem_process_seqs] Processed 685640 reads in 77.466 CPU sec, 2.460 real sec
sorting block 4, #reads = 1072877 mem = 536878612 peak = 851144704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284163, 0, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 264, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515)
[M::mem_pestat] mean and std.dev: (213.36, 77.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 634)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (314.54, 151.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (115, 121, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (157.78, 74.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688874 sequences (100000228 bp) in 1.604 CPU sec, 1.718 real sec...
[M::process] read 688826 sequences (100000059 bp) in 1.210 CPU sec, 1.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285960, 1, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 227, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (220.08, 73.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 279, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (299.37, 144.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 685812 reads in 123.524 CPU sec, 3.936 real sec
sorting block 5, #reads = 1072374 mem = 536878851 peak = 851144704
[M::mem_process_seqs] Processed 689862 reads in 77.262 CPU sec, 2.461 real sec
sorting block 6, #reads = 1074206 mem = 536879282 peak = 851144704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285705, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (303.54, 145.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687770 sequences (100000113 bp) in 1.826 CPU sec, 1.946 real sec...
[M::process] read 687272 sequences (100000095 bp) in 1.220 CPU sec, 1.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286271, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 286, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (305.25, 146.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688874 reads in 122.364 CPU sec, 3.886 real sec
[M::mem_process_seqs] Processed 688826 reads in 76.301 CPU sec, 2.422 real sec
sorting block 7, #reads = 1075244 mem = 536881350 peak = 851144704
[M::process] read 687050 sequences (100000072 bp) in 1.680 CPU sec, 1.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285164, 6, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 266, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (241.36, 78.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 686434 sequences (100000122 bp) in 1.260 CPU sec, 1.256 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (311.60, 149.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 687272 reads in 66.287 CPU sec, 2.090 real sec
sorting block 8, #reads = 1075611 mem = 536877104 peak = 851144704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285772, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (308.16, 146.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285016, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (313.55, 150.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686336 sequences (100000222 bp) in 1.547 CPU sec, 1.552 real sec...
[M::mem_process_seqs] Processed 687770 reads in 188.675 CPU sec, 5.993 real sec
[M::mem_process_seqs] Processed 687050 reads in 88.960 CPU sec, 2.836 real sec
sorting block 9, #reads = 1074045 mem = 536878000 peak = 851144704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284800, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (314.61, 150.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686456 sequences (100000186 bp) in 2.132 CPU sec, 2.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284691, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 292, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (312.74, 149.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 182, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461)
[M::mem_pestat] mean and std.dev: (173.40, 56.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 563)
[M::mem_pestat] skip orientation RR
[M::process] read 686398 sequences (100000145 bp) in 1.270 CPU sec, 1.267 real sec...
[M::mem_process_seqs] Processed 686434 reads in 124.574 CPU sec, 3.954 real sec
[M::mem_process_seqs] Processed 686336 reads in 78.680 CPU sec, 2.486 real sec
sorting block 10, #reads = 1073767 mem = 536876802 peak = 851144704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284826, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (312.04, 150.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689982 sequences (100000220 bp) in 1.851 CPU sec, 1.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284222, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (312.99, 151.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688856 sequences (100000007 bp) in 1.290 CPU sec, 1.292 real sec...
[M::mem_process_seqs] Processed 686456 reads in 126.084 CPU sec, 4.011 real sec
[M::mem_process_seqs] Processed 686398 reads in 79.609 CPU sec, 2.517 real sec
sorting block 11, #reads = 1073545 mem = 536880332 peak = 851144704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286060, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (299.57, 144.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688406 sequences (100000138 bp) in 2.118 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285844, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (305.09, 147.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687284 sequences (100000067 bp) in 1.290 CPU sec, 1.284 real sec...
[M::mem_process_seqs] Processed 689982 reads in 125.080 CPU sec, 3.972 real sec
sorting block 12, #reads = 1072574 mem = 536880674 peak = 851144704
[M::mem_process_seqs] Processed 688856 reads in 78.430 CPU sec, 2.480 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285500, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 287, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (306.97, 147.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1075671 mem = 536878846 peak = 915415040
[M::process] read 687068 sequences (100000078 bp) in 2.126 CPU sec, 2.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285666, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (309.81, 147.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685984 sequences (100000029 bp) in 1.320 CPU sec, 1.322 real sec...
[M::mem_process_seqs] Processed 688406 reads in 125.484 CPU sec, 3.985 real sec
[M::mem_process_seqs] Processed 687284 reads in 79.061 CPU sec, 2.501 real sec
sorting block 14, #reads = 1074652 mem = 536878844 peak = 915415040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285281, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 204, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (209.47, 61.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 651)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (312.90, 149.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685608 sequences (100000286 bp) in 2.293 CPU sec, 2.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284651, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (314.64, 150.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 247, 4156)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12118)
[M::mem_pestat] mean and std.dev: (1282.45, 1760.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16099)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687068 reads in 122.660 CPU sec, 3.893 real sec
[M::mem_process_seqs] Processed 685984 reads in 78.003 CPU sec, 2.457 real sec
[M::process] read 686364 sequences (100000203 bp) in 1.545 CPU sec, 1.585 real sec...
sorting block 15, #reads = 1074707 mem = 536877604 peak = 915415040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284183, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 297, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (316.14, 150.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686690 sequences (100000186 bp) in 2.073 CPU sec, 2.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284117, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (312.41, 149.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685608 reads in 124.984 CPU sec, 3.977 real sec
[M::process] read 686310 sequences (100000227 bp) in 1.350 CPU sec, 1.345 real sec...
[M::mem_process_seqs] Processed 686364 reads in 79.545 CPU sec, 2.516 real sec
sorting block 16, #reads = 1073069 mem = 536878769 peak = 915415040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284937, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 255, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477)
[M::mem_pestat] mean and std.dev: (196.00, 57.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (311.59, 150.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 17, #reads = 1072482 mem = 536877061 peak = 915415040
[M::process] read 689526 sequences (100000013 bp) in 2.188 CPU sec, 2.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284736, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (313.34, 152.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 153, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (181.55, 83.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686690 reads in 124.852 CPU sec, 3.968 real sec
[M::process] read 689148 sequences (100000070 bp) in 1.330 CPU sec, 1.332 real sec...
[M::mem_process_seqs] Processed 686310 reads in 79.185 CPU sec, 2.500 real sec
sorting block 18, #reads = 1072899 mem = 536876965 peak = 915415040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285842, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 162, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519)
[M::mem_pestat] mean and std.dev: (190.69, 77.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 650)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (300.48, 145.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688052 sequences (100000168 bp) in 1.944 CPU sec, 2.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285965, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (303.60, 146.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (117, 355, 4484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13218)
[M::mem_pestat] mean and std.dev: (1530.90, 1938.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17585)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689526 reads in 124.880 CPU sec, 3.978 real sec
[M::process] read 687396 sequences (100000180 bp) in 1.348 CPU sec, 1.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285323, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 287, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (307.61, 148.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 254, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 479)
[M::mem_pestat] mean and std.dev: (223.17, 64.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 573)
[M::mem_pestat] skip orientation RR
[M::process] read 686564 sequences (100000063 bp) in 1.519 CPU sec, 1.522 real sec...
[M::mem_process_seqs] Processed 688052 reads in 71.531 CPU sec, 2.453 real sec
sorting block 19, #reads = 1075417 mem = 536878420 peak = 915415040
[M::mem_process_seqs] Processed 689148 reads in 168.921 CPU sec, 5.543 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285026, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (309.32, 148.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1075553 mem = 536881000 peak = 978358272
[M::process] read 686428 sequences (100000065 bp) in 2.123 CPU sec, 2.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284834, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (313.09, 149.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687396 reads in 134.733 CPU sec, 4.283 real sec
[M::mem_process_seqs] Processed 686564 reads in 78.950 CPU sec, 2.497 real sec
[M::process] read 686346 sequences (100000096 bp) in 1.624 CPU sec, 1.716 real sec...
sorting block 21, #reads = 1074477 mem = 536880510 peak = 978358272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284654, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 182, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467)
[M::mem_pestat] mean and std.dev: (185.60, 67.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 575)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (313.12, 149.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686528 sequences (100000090 bp) in 2.046 CPU sec, 2.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284721, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (312.33, 150.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686428 reads in 125.417 CPU sec, 3.994 real sec
[M::mem_process_seqs] Processed 686346 reads in 79.744 CPU sec, 2.519 real sec
[M::process] read 686518 sequences (100000154 bp) in 1.460 CPU sec, 1.493 real sec...
sorting block 22, #reads = 1073225 mem = 536886454 peak = 978358272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285559, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (309.53, 148.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686332 sequences (100000109 bp) in 2.159 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284365, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 207, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 415)
[M::mem_pestat] mean and std.dev: (202.64, 38.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 495)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 288, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (310.11, 149.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 279, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (286.33, 145.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1409)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686528 reads in 125.398 CPU sec, 3.990 real sec
[M::process] read 692282 sequences (100000167 bp) in 1.441 CPU sec, 1.468 real sec...
[M::mem_process_seqs] Processed 686518 reads in 79.807 CPU sec, 2.517 real sec
sorting block 23, #reads = 1073493 mem = 536878450 peak = 978358272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284832, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (313.10, 151.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 267, 932)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2372)
[M::mem_pestat] mean and std.dev: (474.18, 328.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3092)
[M::mem_pestat] skip orientation RR
sorting block 24, #reads = 1073194 mem = 536878983 peak = 978358272
[M::process] read 691522 sequences (100000272 bp) in 2.218 CPU sec, 2.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287273, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (293.11, 145.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 270, 3319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9517)
[M::mem_pestat] mean and std.dev: (1297.50, 1437.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12616)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686332 reads in 127.538 CPU sec, 4.055 real sec
[M::process] read 689680 sequences (100000107 bp) in 1.377 CPU sec, 1.384 real sec...
[M::mem_process_seqs] Processed 692282 reads in 82.662 CPU sec, 2.614 real sec
sorting block 25, #reads = 1072939 mem = 536885532 peak = 978358272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286953, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 274, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (296.66, 145.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688968 sequences (100000200 bp) in 2.198 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287029, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (95, 116, 176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338)
[M::mem_pestat] mean and std.dev: (129.18, 35.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 419)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (301.78, 146.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 218, 1519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4339)
[M::mem_pestat] mean and std.dev: (317.82, 391.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5749)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689128 sequences (100000158 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_process_seqs] Processed 691522 reads in 127.526 CPU sec, 4.048 real sec
[M::mem_process_seqs] Processed 689680 reads in 79.306 CPU sec, 2.506 real sec
sorting block 26, #reads = 1074827 mem = 536879231 peak = 978358272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286388, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (304.52, 147.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688662 sequences (100000154 bp) in 1.960 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286372, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 285, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (304.82, 147.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 358, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070)
[M::mem_pestat] mean and std.dev: (286.90, 140.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1379)
[M::mem_pestat] skip orientation RR
[M::process] read 687528 sequences (100000251 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 688968 reads in 129.084 CPU sec, 4.107 real sec
[M::mem_process_seqs] Processed 689128 reads in 82.487 CPU sec, 2.612 real sec
sorting block 27, #reads = 1074896 mem = 536882370 peak = 978358272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286107, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 148, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (202.67, 115.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 286, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (306.45, 148.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687320 sequences (100000285 bp) in 2.158 CPU sec, 2.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285583, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 285, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (302.73, 142.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687154 sequences (100000189 bp) in 1.330 CPU sec, 1.335 real sec...
[M::mem_process_seqs] Processed 688662 reads in 134.263 CPU sec, 4.269 real sec
sorting block 28, #reads = 1073215 mem = 536886481 peak = 978358272
[M::mem_process_seqs] Processed 687528 reads in 82.673 CPU sec, 2.621 real sec
sorting block 29, #reads = 1071296 mem = 536876997 peak = 1040289792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285750, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 169, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (251.14, 189.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (304.46, 143.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686818 sequences (100000214 bp) in 2.186 CPU sec, 2.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285756, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (304.99, 143.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (290, 325, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (368.00, 164.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RR
[M::process] read 692696 sequences (100000228 bp) in 1.380 CPU sec, 1.377 real sec...
[M::mem_process_seqs] Processed 687320 reads in 127.296 CPU sec, 4.044 real sec
[M::mem_process_seqs] Processed 687154 reads in 80.354 CPU sec, 2.540 real sec
sorting block 30, #reads = 1072042 mem = 536879357 peak = 1040289792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285259, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 172, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (192.08, 74.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 719)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (306.69, 145.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 690750 sequences (100000100 bp) in 2.232 CPU sec, 2.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287879, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 269, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (290.17, 141.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690130 sequences (100000055 bp) in 1.350 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 686818 reads in 131.647 CPU sec, 4.181 real sec
[M::mem_process_seqs] Processed 692696 reads in 82.508 CPU sec, 2.611 real sec
sorting block 31, #reads = 1071755 mem = 536876739 peak = 1040289792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286626, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 275, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (295.08, 141.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689104 sequences (100000198 bp) in 2.300 CPU sec, 2.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285947, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 279, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (298.37, 143.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688660 sequences (100000219 bp) in 1.350 CPU sec, 1.344 real sec...
[M::mem_process_seqs] Processed 690750 reads in 131.419 CPU sec, 4.172 real sec
sorting block 32, #reads = 1075378 mem = 536878965 peak = 1040289792
[M::mem_process_seqs] Processed 690130 reads in 83.239 CPU sec, 2.632 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286392, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (301.26, 144.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1073328 mem = 536877614 peak = 1040289792
[M::process] read 688540 sequences (100000168 bp) in 2.047 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285817, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (302.69, 144.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 210, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (229.73, 84.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RR
[M::process] read 687728 sequences (100000011 bp) in 1.320 CPU sec, 1.322 real sec...
[M::mem_process_seqs] Processed 689104 reads in 129.229 CPU sec, 4.111 real sec
[M::mem_process_seqs] Processed 688660 reads in 82.716 CPU sec, 2.614 real sec
sorting block 34, #reads = 1073148 mem = 536877348 peak = 1040289792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286133, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (303.23, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687040 sequences (100000267 bp) in 2.217 CPU sec, 2.337 real sec...
[M::process] read 686798 sequences (100000186 bp) in 1.340 CPU sec, 1.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285877, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (305.79, 145.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688540 reads in 141.964 CPU sec, 4.514 real sec
[M::mem_process_seqs] Processed 687728 reads in 91.385 CPU sec, 2.899 real sec
sorting block 35, #reads = 1070943 mem = 536880071 peak = 1040289792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285765, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (308.70, 146.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688774 sequences (100000153 bp) in 2.293 CPU sec, 2.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285380, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (310.03, 147.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691226 sequences (100000280 bp) in 1.320 CPU sec, 1.328 real sec...
[M::mem_process_seqs] Processed 687040 reads in 130.963 CPU sec, 4.155 real sec
[M::mem_process_seqs] Processed 686798 reads in 81.784 CPU sec, 2.589 real sec
sorting block 36, #reads = 1068286 mem = 536879471 peak = 1040289792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285895, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 282, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (303.75, 147.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689768 sequences (100000106 bp) in 2.236 CPU sec, 2.348 real sec...
[M::process] read 689392 sequences (100000228 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287389, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 273, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (293.59, 142.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688774 reads in 139.812 CPU sec, 4.444 real sec
sorting block 37, #reads = 1072245 mem = 536880462 peak = 1040289792
[M::mem_process_seqs] Processed 691226 reads in 88.477 CPU sec, 2.804 real sec
sorting block 38, #reads = 1071230 mem = 536883205 peak = 1089363968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286422, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (109, 128, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (187.36, 95.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 279, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (298.28, 143.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 270, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (270.57, 91.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688214 sequences (100000172 bp) in 2.309 CPU sec, 2.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286730, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 194, 3903)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11409)
[M::mem_pestat] mean and std.dev: (1289.60, 1711.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15162)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (300.22, 143.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688642 sequences (100000292 bp) in 1.340 CPU sec, 1.336 real sec...
[M::mem_process_seqs] Processed 689768 reads in 129.756 CPU sec, 4.117 real sec
[M::mem_process_seqs] Processed 689392 reads in 83.048 CPU sec, 2.623 real sec
sorting block 39, #reads = 1073868 mem = 536878877 peak = 1089363968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286027, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 285, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.36, 144.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687416 sequences (100000026 bp) in 2.097 CPU sec, 2.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286603, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.21, 144.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 210, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (196.55, 78.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RR
[M::process] read 686648 sequences (100000201 bp) in 1.340 CPU sec, 1.343 real sec...
[M::mem_process_seqs] Processed 688214 reads in 130.526 CPU sec, 4.152 real sec
[M::mem_process_seqs] Processed 688642 reads in 84.059 CPU sec, 2.660 real sec
sorting block 40, #reads = 1073926 mem = 536878843 peak = 1089363968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285572, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (308.12, 146.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686638 sequences (100000273 bp) in 2.255 CPU sec, 2.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285463, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (309.54, 146.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686624 sequences (100000165 bp) in 1.340 CPU sec, 1.345 real sec...
[M::mem_process_seqs] Processed 687416 reads in 133.555 CPU sec, 4.244 real sec
sorting block 41, #reads = 1072131 mem = 536878117 peak = 1089363968
[M::mem_process_seqs] Processed 686648 reads in 84.099 CPU sec, 2.666 real sec
sorting block 42, #reads = 1070134 mem = 536878070 peak = 1089363968
[M::process] read 689280 sequences (100000166 bp) in 2.328 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285050, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (309.38, 147.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 284, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (276.38, 101.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] skip orientation RR
[M::process] read 691344 sequences (100000082 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_process_seqs] Processed 686624 reads in 78.183 CPU sec, 2.470 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285505, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (309.55, 147.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286318, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (301.77, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690032 sequences (100000004 bp) in 1.400 CPU sec, 1.404 real sec...
[M::mem_process_seqs] Processed 686638 reads in 213.241 CPU sec, 6.773 real sec
sorting block 43, #reads = 1067577 mem = 536880832 peak = 1089363968
[M::mem_process_seqs] Processed 689280 reads in 101.179 CPU sec, 3.233 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287029, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 273, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (294.95, 143.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1068357 mem = 536878961 peak = 1089363968
[M::process] read 689532 sequences (100000103 bp) in 2.191 CPU sec, 2.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286452, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 279, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (300.52, 146.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691344 reads in 131.098 CPU sec, 4.167 real sec
[M::process] read 688252 sequences (100000232 bp) in 1.360 CPU sec, 1.362 real sec...
[M::mem_process_seqs] Processed 690032 reads in 82.278 CPU sec, 2.600 real sec
sorting block 45, #reads = 1073439 mem = 536877510 peak = 1089363968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286241, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 171, 171)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 281)
[M::mem_pestat] mean and std.dev: (144.83, 29.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 336)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (303.81, 147.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687520 sequences (100000050 bp) in 2.228 CPU sec, 2.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285951, 0, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 185, 185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327)
[M::mem_pestat] mean and std.dev: (151.30, 50.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 398)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (308.39, 148.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 345, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (322.00, 133.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687144 sequences (100000135 bp) in 1.360 CPU sec, 1.354 real sec...
[M::mem_process_seqs] Processed 689532 reads in 130.358 CPU sec, 4.135 real sec
[M::mem_process_seqs] Processed 688252 reads in 84.363 CPU sec, 2.669 real sec
sorting block 46, #reads = 1072839 mem = 536879798 peak = 1089363968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285595, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 199, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (182.80, 48.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (310.49, 149.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686562 sequences (100000136 bp) in 2.182 CPU sec, 2.294 real sec...
[M::process] read 686472 sequences (100000191 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285549, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (309.98, 148.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687520 reads in 138.183 CPU sec, 4.390 real sec
sorting block 47, #reads = 1069864 mem = 536879682 peak = 1089363968
[M::mem_process_seqs] Processed 687144 reads in 85.337 CPU sec, 2.706 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284976, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 288, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (307.32, 145.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686238 sequences (100000290 bp) in 2.249 CPU sec, 2.405 real sec...
[M::process] read 194570 sequences (28343791 bp) in 0.580 CPU sec, 0.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285079, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 205, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (193.20, 77.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 569)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 287, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (307.18, 145.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 154, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (173.20, 62.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 529)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686562 reads in 130.435 CPU sec, 4.162 real sec
sorting block 48, #reads = 1069427 mem = 536882078 peak = 1089363968
[M::mem_process_seqs] Processed 686472 reads in 82.037 CPU sec, 2.621 real sec
sorting block 49, #reads = 1069846 mem = 536882747 peak = 1089363968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284937, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 288, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (308.87, 147.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 80793, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 290, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (310.63, 149.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686238 reads in 85.536 CPU sec, 2.780 real sec
[M::mem_process_seqs] Processed 194570 reads in 31.417 CPU sec, 1.022 real sec
sorting block 50, #reads = 1070327 mem = 536879498 peak = 1089363968
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 54547790
overall: 69781999616 mem 6925.814 user 328.421 sys 604.698 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0002_B23KGCJLT4_1.L007\tPU:L007\tSM:NA12878_c_0002_gDNA_0002_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0002_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 604.735 sec; CPU: 7254.238 sec
sorting block 51, #reads = 98535 mem = 49432257 peak = 1089363968
uniq header count 1
merging 52 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2849981912
output reads: 54817579
bam_mem_sort: 52 calls 20.145 user 0.208 sys 20.504 real
bam_write: 52 calls 15.184 user 0.458 sys 15.790 real
execute: 1 calls 10.330 user 0.854 sys 619.202 real
merge_files: 1 calls 10.303 user 0.839 sys 14.359 real
parse_chunk: 818 calls 27.687 user 6.981 sys 35.164 real
read_chunk: 2210 calls 18.890 user 17.180 sys 185.290 real
sort_block: 1 calls 14.699 user 16.675 sys 178.914 real
write_chunk: 1390 calls 9.994 user 0.658 sys 13.733 real
overall: 2518593536 mem 566.096 user 31.942 sys 619.222 real
ls: cannot access '*.cram': No such file or directory