.command.err - Workdir - DAQ:SENTIEON_BWAMEM (NA12878_c_0002_gDNA_0003_B23KGCJLT4_1;L001;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/76/d36857a3624e9d93f729362db05d1d/.command.err
Size: 51.2 KB
Task: DAQ:SENTIEON_BWAMEM (NA12878_c_0002_gDNA_0003_B23KGCJLT4_1;L001;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0003_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1638.468 CPU sec, 1136.581 real sec
[M::process] read 681450 sequences (100000277 bp) in 0.878 CPU sec, 0.886 real sec...
[M::process] read 681830 sequences (100000102 bp) in 1.143 CPU sec, 1.191 real sec...
[M::process] read 681114 sequences (100000301 bp) in 1.142 CPU sec, 1.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283145, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 200, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (82, 342)
[M::mem_pestat] mean and std.dev: (202.36, 54.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 420)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (335.04, 158.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 216, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (204.67, 68.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680024 sequences (100000063 bp) in 1.090 CPU sec, 1.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283202, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (333.16, 159.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 279, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (218.50, 73.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282896, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 318, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (340.54, 164.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681450 reads in 211.508 CPU sec, 6.809 real sec
[M::mem_process_seqs] Processed 681830 reads in 136.345 CPU sec, 4.401 real sec
[M::mem_process_seqs] Processed 681114 reads in 147.821 CPU sec, 4.791 real sec
sorting block 0, #reads = 1069267 mem = 536877445 peak = 594685952
[M::process] read 680368 sequences (100000088 bp) in 1.577 CPU sec, 1.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281587, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (343.20, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680792 sequences (100000213 bp) in 1.151 CPU sec, 1.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282173, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (340.11, 162.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680710 sequences (100000017 bp) in 1.010 CPU sec, 1.014 real sec...
[M::mem_process_seqs] Processed 680024 reads in 128.407 CPU sec, 4.081 real sec
sorting block 1, #reads = 1069420 mem = 536878946 peak = 811245568
[M::mem_process_seqs] Processed 680368 reads in 80.409 CPU sec, 2.549 real sec
sorting block 2, #reads = 1067712 mem = 536881093 peak = 881573888
[M::process] read 681126 sequences (100000267 bp) in 1.427 CPU sec, 1.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282343, 0, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 316, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (339.16, 163.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (347, 451, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (79, 749)
[M::mem_pestat] mean and std.dev: (377.67, 131.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RR
[M::process] read 680610 sequences (100000252 bp) in 1.152 CPU sec, 1.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283283, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (341.09, 160.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282588, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (340.10, 162.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680792 reads in 179.742 CPU sec, 5.758 real sec
[M::mem_process_seqs] Processed 680710 reads in 132.105 CPU sec, 4.245 real sec
[M::mem_process_seqs] Processed 681126 reads in 143.618 CPU sec, 4.637 real sec
sorting block 3, #reads = 1068969 mem = 536880856 peak = 881573888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282582, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (344.51, 165.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680040 sequences (100000197 bp) in 1.913 CPU sec, 2.044 real sec...
[M::mem_process_seqs] Processed 680610 reads in 71.715 CPU sec, 2.368 real sec
sorting block 4, #reads = 1068713 mem = 536879646 peak = 881573888
[M::process] read 680350 sequences (100000077 bp) in 1.787 CPU sec, 1.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282178, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (344.04, 164.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680432 sequences (100000141 bp) in 1.139 CPU sec, 1.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282769, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 245, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (21, 416)
[M::mem_pestat] mean and std.dev: (205.44, 77.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 514)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (342.36, 165.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 184, 1303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3661)
[M::mem_pestat] mean and std.dev: (961.75, 1174.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5659)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680854 sequences (100000054 bp) in 1.124 CPU sec, 1.130 real sec...
[M::mem_process_seqs] Processed 680040 reads in 129.684 CPU sec, 4.113 real sec
sorting block 5, #reads = 1068300 mem = 536879130 peak = 881573888
[M::mem_process_seqs] Processed 680350 reads in 81.213 CPU sec, 2.575 real sec
sorting block 6, #reads = 1068173 mem = 536878987 peak = 892805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282114, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 321, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (342.39, 165.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681152 sequences (100000008 bp) in 1.853 CPU sec, 1.976 real sec...
[M::process] read 680020 sequences (100000000 bp) in 1.150 CPU sec, 1.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282982, 4, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (342.11, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 211, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (196.31, 84.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680432 reads in 128.275 CPU sec, 4.092 real sec
[M::mem_process_seqs] Processed 680854 reads in 80.175 CPU sec, 2.559 real sec
sorting block 7, #reads = 1067893 mem = 536881582 peak = 892805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283597, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 175, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (211.23, 100.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (340.41, 162.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680036 sequences (100000247 bp) in 1.796 CPU sec, 1.885 real sec...
[M::process] read 680546 sequences (100000091 bp) in 1.190 CPU sec, 1.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282780, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 326, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (346.34, 166.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681152 reads in 126.663 CPU sec, 4.043 real sec
[M::mem_process_seqs] Processed 680020 reads in 80.335 CPU sec, 2.562 real sec
sorting block 8, #reads = 1069391 mem = 536884608 peak = 892805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282308, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (345.02, 165.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680456 sequences (100000211 bp) in 1.987 CPU sec, 2.125 real sec...
[M::process] read 681088 sequences (100000158 bp) in 1.170 CPU sec, 1.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283146, 0, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 265, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (224.22, 58.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 637)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (342.60, 166.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 248, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (253.53, 102.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680036 reads in 127.819 CPU sec, 4.077 real sec
[M::mem_process_seqs] Processed 680546 reads in 79.509 CPU sec, 2.533 real sec
sorting block 9, #reads = 1067900 mem = 536880183 peak = 892805120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282846, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (343.78, 166.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 320, 630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388)
[M::mem_pestat] mean and std.dev: (369.45, 270.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1767)
[M::mem_pestat] skip orientation RR
[M::process] read 681324 sequences (100000172 bp) in 1.994 CPU sec, 2.135 real sec...
[M::process] read 680678 sequences (100000131 bp) in 1.250 CPU sec, 1.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282594, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (341.61, 165.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680456 reads in 128.477 CPU sec, 4.098 real sec
sorting block 10, #reads = 1069224 mem = 536880051 peak = 892805120
[M::mem_process_seqs] Processed 681088 reads in 80.420 CPU sec, 2.562 real sec
sorting block 11, #reads = 1069156 mem = 536878344 peak = 902504448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282962, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (336.43, 161.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680450 sequences (100000197 bp) in 2.010 CPU sec, 2.164 real sec...
[M::process] read 680442 sequences (100000125 bp) in 1.250 CPU sec, 1.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282542, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (340.12, 164.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 190, 1063)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2869)
[M::mem_pestat] mean and std.dev: (545.90, 645.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3772)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681324 reads in 129.541 CPU sec, 4.134 real sec
[M::mem_process_seqs] Processed 680678 reads in 81.803 CPU sec, 2.605 real sec
sorting block 12, #reads = 1069734 mem = 536875979 peak = 902504448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282486, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 181, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (222.11, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (339.95, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681050 sequences (100000039 bp) in 2.010 CPU sec, 2.146 real sec...
[M::process] read 681868 sequences (100000056 bp) in 1.250 CPU sec, 1.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282623, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 188, 832)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144)
[M::mem_pestat] mean and std.dev: (379.15, 295.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2800)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (340.21, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 198, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492)
[M::mem_pestat] mean and std.dev: (167.92, 64.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 615)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680450 reads in 131.053 CPU sec, 4.182 real sec
[M::mem_process_seqs] Processed 680442 reads in 81.637 CPU sec, 2.597 real sec
sorting block 13, #reads = 1067718 mem = 536885079 peak = 902504448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282990, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (337.80, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 207, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (239.33, 158.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] skip orientation RR
[M::process] read 682082 sequences (100000137 bp) in 2.045 CPU sec, 2.193 real sec...
[M::process] read 682010 sequences (100000127 bp) in 1.260 CPU sec, 1.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283501, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (331.28, 158.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681050 reads in 129.065 CPU sec, 4.118 real sec
[M::mem_process_seqs] Processed 681868 reads in 80.225 CPU sec, 2.553 real sec
sorting block 14, #reads = 1068310 mem = 536879559 peak = 902504448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283359, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 309, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (328.65, 156.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1069628 mem = 536880108 peak = 902504448
[M::process] read 681276 sequences (100000068 bp) in 2.064 CPU sec, 2.207 real sec...
[M::process] read 680954 sequences (100000021 bp) in 1.290 CPU sec, 1.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283467, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (109, 162, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (173.50, 54.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 573)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (328.47, 156.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 267, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (260.44, 143.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682082 reads in 131.015 CPU sec, 4.180 real sec
[M::mem_process_seqs] Processed 682010 reads in 81.867 CPU sec, 2.607 real sec
sorting block 16, #reads = 1069975 mem = 536878978 peak = 902504448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282942, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (326.84, 153.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681274 sequences (100000197 bp) in 2.175 CPU sec, 2.312 real sec...
[M::process] read 681620 sequences (100000257 bp) in 1.250 CPU sec, 1.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283155, 0, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 119, 164)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280)
[M::mem_pestat] mean and std.dev: (139.50, 43.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 338)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (329.20, 153.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 198, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (213.06, 113.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 685)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681276 reads in 132.079 CPU sec, 4.214 real sec
[M::mem_process_seqs] Processed 680954 reads in 82.329 CPU sec, 2.622 real sec
sorting block 17, #reads = 1069476 mem = 536883509 peak = 902504448
[M::process] read 682144 sequences (100000015 bp) in 2.191 CPU sec, 2.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283555, 0, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (110, 114, 168)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284)
[M::mem_pestat] mean and std.dev: (121.44, 26.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 342)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 310, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (328.60, 154.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681738 sequences (100000010 bp) in 1.270 CPU sec, 1.269 real sec...
[M::mem_process_seqs] Processed 681620 reads in 69.844 CPU sec, 2.211 real sec
sorting block 18, #reads = 1068537 mem = 536879073 peak = 902504448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283040, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 311, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (332.50, 159.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283634, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 178, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556)
[M::mem_pestat] mean and std.dev: (217.31, 72.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (323.18, 152.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680912 sequences (100000001 bp) in 1.309 CPU sec, 1.313 real sec...
[M::mem_process_seqs] Processed 681274 reads in 201.054 CPU sec, 6.413 real sec
[M::mem_process_seqs] Processed 682144 reads in 93.434 CPU sec, 2.998 real sec
sorting block 19, #reads = 1068215 mem = 536880030 peak = 902504448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283888, 3, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (327.19, 155.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 234, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (234.40, 88.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] skip orientation RR
[M::process] read 680750 sequences (100000027 bp) in 2.293 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282737, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 312, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (331.16, 155.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681168 sequences (100000003 bp) in 1.280 CPU sec, 1.278 real sec...
[M::mem_process_seqs] Processed 681738 reads in 130.140 CPU sec, 4.157 real sec
[M::mem_process_seqs] Processed 680912 reads in 81.800 CPU sec, 2.604 real sec
sorting block 20, #reads = 1070134 mem = 536877066 peak = 902504448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282917, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (335.87, 159.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681468 sequences (100000178 bp) in 2.186 CPU sec, 2.338 real sec...
[M::process] read 681754 sequences (100000178 bp) in 1.250 CPU sec, 1.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282965, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 261, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453)
[M::mem_pestat] mean and std.dev: (199.12, 56.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 549)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 313, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (336.16, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 417, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (314.15, 143.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1511)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680750 reads in 132.944 CPU sec, 4.241 real sec
sorting block 21, #reads = 1069048 mem = 536878870 peak = 902504448
[M::mem_process_seqs] Processed 681168 reads in 85.224 CPU sec, 2.714 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283055, 0, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (332.98, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 224, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (199.07, 85.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1067413 mem = 536878778 peak = 945741824
[M::process] read 681262 sequences (100000033 bp) in 2.060 CPU sec, 2.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283207, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (326.98, 155.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680762 sequences (100000099 bp) in 1.270 CPU sec, 1.272 real sec...
[M::mem_process_seqs] Processed 681468 reads in 127.002 CPU sec, 4.053 real sec
[M::mem_process_seqs] Processed 681754 reads in 80.357 CPU sec, 2.554 real sec
sorting block 23, #reads = 1069679 mem = 536878576 peak = 945741824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283293, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (329.24, 156.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680512 sequences (100000025 bp) in 2.125 CPU sec, 2.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282717, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 164, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 372)
[M::mem_pestat] mean and std.dev: (169.69, 49.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 446)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 311, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (330.25, 154.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 331, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 567)
[M::mem_pestat] mean and std.dev: (299.18, 56.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 668)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681188 sequences (100000202 bp) in 1.320 CPU sec, 1.319 real sec...
[M::mem_process_seqs] Processed 681262 reads in 128.469 CPU sec, 4.093 real sec
[M::mem_process_seqs] Processed 680762 reads in 80.517 CPU sec, 2.557 real sec
sorting block 24, #reads = 1069763 mem = 536879817 peak = 945741824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282549, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 315, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (335.68, 159.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682356 sequences (100000249 bp) in 2.168 CPU sec, 2.318 real sec...
[M::process] read 682118 sequences (100000013 bp) in 1.290 CPU sec, 1.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283068, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (337.20, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680512 reads in 136.230 CPU sec, 4.341 real sec
[M::mem_process_seqs] Processed 681188 reads in 85.952 CPU sec, 2.731 real sec
sorting block 25, #reads = 1067280 mem = 536881059 peak = 945741824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283577, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 311, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (335.94, 165.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 194, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (205.33, 71.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RR
[M::process] read 681148 sequences (100000018 bp) in 2.027 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283496, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 310, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (332.17, 160.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 222, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (86, 401)
[M::mem_pestat] mean and std.dev: (218.11, 47.17)
[M::mem_pestat] low and high boundaries for proper pairs: (23, 464)
[M::mem_pestat] skip orientation RR
[M::process] read 680800 sequences (100000071 bp) in 1.260 CPU sec, 1.258 real sec...
[M::mem_process_seqs] Processed 682356 reads in 129.735 CPU sec, 4.136 real sec
sorting block 26, #reads = 1066896 mem = 536879471 peak = 945741824
[M::mem_process_seqs] Processed 682118 reads in 80.640 CPU sec, 2.557 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283008, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (336.21, 161.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 382, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (384.00, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1499)
[M::mem_pestat] skip orientation RR
sorting block 27, #reads = 1070275 mem = 536880270 peak = 1011363840
[M::process] read 680152 sequences (100000051 bp) in 2.175 CPU sec, 2.312 real sec...
[M::process] read 504458 sequences (74173092 bp) in 1.030 CPU sec, 1.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282893, 0, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 314, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (335.10, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (295, 777, 1338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3424)
[M::mem_pestat] mean and std.dev: (769.43, 510.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4467)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681148 reads in 128.999 CPU sec, 4.111 real sec
[M::mem_process_seqs] Processed 680800 reads in 81.061 CPU sec, 2.579 real sec
sorting block 28, #reads = 1067552 mem = 536877089 peak = 1011363840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282572, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 315, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (336.63, 159.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 772, 901)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2139)
[M::mem_pestat] mean and std.dev: (564.83, 301.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2758)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 209763, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (338.92, 163.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680152 reads in 111.131 CPU sec, 3.607 real sec
[M::mem_process_seqs] Processed 504458 reads in 61.912 CPU sec, 2.002 real sec
sorting block 29, #reads = 1068674 mem = 536879252 peak = 1011363840
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 32512716
overall: 69684633600 mem 4735.743 user 293.242 sys 1252.735 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0003_B23KGCJLT4_1.L001\tPU:L001\tSM:NA12878_c_0002_gDNA_0003_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0003_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0003_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0003_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 1252.770 sec; CPU: 5028.988 sec
sorting block 30, #reads = 608751 mem = 306168005 peak = 1011363840
uniq header count 1
merging 31 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 1636482020
output reads: 32671176
bam_mem_sort: 31 calls 11.044 user 0.065 sys 11.184 real
bam_write: 31 calls 14.221 user 0.547 sys 14.936 real
execute: 1 calls 5.627 user 0.340 sys 1261.558 real
merge_files: 1 calls 5.617 user 0.312 sys 8.083 real
parse_chunk: 490 calls 14.943 user 3.733 sys 19.100 real
read_chunk: 1323 calls 10.570 user 12.178 sys 111.919 real
sort_block: 1 calls 9.326 user 11.746 sys 109.178 real
write_chunk: 831 calls 5.336 user 0.195 sys 7.527 real
overall: 2338721792 mem 310.182 user 21.007 sys 1261.579 real
ls: cannot access '*.cram': No such file or directory