cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0001_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1600.454 CPU sec, 773.127 real sec
[M::process] read 690536 sequences (100000021 bp) in 0.908 CPU sec, 0.917 real sec...
[M::process] read 689178 sequences (100000147 bp) in 1.158 CPU sec, 1.177 real sec...
[M::process] read 688440 sequences (100000032 bp) in 1.133 CPU sec, 1.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286184, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 270, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (293.09, 143.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688020 sequences (100000089 bp) in 1.093 CPU sec, 1.094 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285432, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (298.24, 145.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 151, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (210.55, 130.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285387, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 196, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (162.38, 60.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 703)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 279, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (301.03, 146.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 690536 reads in 200.871 CPU sec, 6.426 real sec
[M::mem_process_seqs] Processed 689178 reads in 133.935 CPU sec, 4.322 real sec
[M::mem_process_seqs] Processed 688440 reads in 145.140 CPU sec, 4.698 real sec
sorting block 0, #reads = 1074288 mem = 536876646 peak = 604237824
[M::process] read 687454 sequences (100000175 bp) in 1.624 CPU sec, 1.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285842, 1, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 281, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (303.84, 147.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 223, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480)
[M::mem_pestat] mean and std.dev: (188.57, 49.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 589)
[M::mem_pestat] skip orientation RR
[M::process] read 687882 sequences (100000162 bp) in 1.230 CPU sec, 1.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285707, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 187, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 328)
[M::mem_pestat] mean and std.dev: (169.80, 43.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 385)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (307.11, 149.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686016 sequences (100000123 bp) in 1.090 CPU sec, 1.091 real sec...
[M::mem_process_seqs] Processed 688020 reads in 125.833 CPU sec, 3.997 real sec
sorting block 1, #reads = 1074493 mem = 536881612 peak = 835448832
[M::mem_process_seqs] Processed 687454 reads in 78.712 CPU sec, 2.493 real sec
sorting block 2, #reads = 1075839 mem = 536883286 peak = 891367424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285567, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 262, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452)
[M::mem_pestat] mean and std.dev: (216.40, 56.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 547)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (305.21, 150.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 232, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (231.89, 107.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685828 sequences (100000194 bp) in 1.641 CPU sec, 1.740 real sec...
[M::process] read 685644 sequences (100000135 bp) in 1.221 CPU sec, 1.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284585, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (308.17, 148.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687882 reads in 126.181 CPU sec, 4.018 real sec
[M::mem_process_seqs] Processed 686016 reads in 78.610 CPU sec, 2.511 real sec
sorting block 3, #reads = 1073674 mem = 536887580 peak = 891367424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284357, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (308.39, 148.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685402 sequences (100000119 bp) in 1.727 CPU sec, 1.836 real sec...
[M::process] read 690342 sequences (100000121 bp) in 1.199 CPU sec, 1.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284434, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (309.15, 148.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685828 reads in 127.103 CPU sec, 4.055 real sec
[M::mem_process_seqs] Processed 685644 reads in 78.700 CPU sec, 2.509 real sec
sorting block 4, #reads = 1072457 mem = 536878894 peak = 891367424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284141, 1, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (312.45, 151.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 167, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339)
[M::mem_pestat] mean and std.dev: (161.54, 35.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 408)
[M::mem_pestat] skip orientation RR
[M::process] read 689648 sequences (100000101 bp) in 1.701 CPU sec, 1.836 real sec...
[M::process] read 688602 sequences (100000054 bp) in 1.211 CPU sec, 1.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286578, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 273, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (296.00, 145.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685402 reads in 128.428 CPU sec, 4.106 real sec
sorting block 5, #reads = 1072313 mem = 536879122 peak = 891367424
[M::mem_process_seqs] Processed 690342 reads in 80.018 CPU sec, 2.548 real sec
sorting block 6, #reads = 1074096 mem = 536881547 peak = 891367424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285935, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (299.27, 146.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688808 sequences (100000132 bp) in 1.885 CPU sec, 1.991 real sec...
[M::process] read 687990 sequences (100000216 bp) in 1.270 CPU sec, 1.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286004, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (302.45, 147.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689648 reads in 127.430 CPU sec, 4.072 real sec
[M::mem_process_seqs] Processed 688602 reads in 78.864 CPU sec, 2.512 real sec
sorting block 7, #reads = 1075561 mem = 536880303 peak = 891367424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285726, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (303.45, 149.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687692 sequences (100000135 bp) in 1.979 CPU sec, 2.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285683, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (307.69, 151.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685762 sequences (100000112 bp) in 1.390 CPU sec, 1.394 real sec...
[M::mem_process_seqs] Processed 688808 reads in 128.487 CPU sec, 4.108 real sec
[M::mem_process_seqs] Processed 687990 reads in 80.470 CPU sec, 2.557 real sec
sorting block 8, #reads = 1075373 mem = 536879944 peak = 891367424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285658, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (308.03, 151.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686342 sequences (100000013 bp) in 2.303 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284749, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (312.32, 150.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687692 reads in 130.119 CPU sec, 4.149 real sec
[M::process] read 686108 sequences (100000016 bp) in 1.410 CPU sec, 1.416 real sec...
[M::mem_process_seqs] Processed 685762 reads in 81.408 CPU sec, 2.587 real sec
sorting block 9, #reads = 1074087 mem = 536878391 peak = 891367424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284834, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 285, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (308.28, 149.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 271, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (244.20, 109.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation RR
[M::process] read 685826 sequences (100000155 bp) in 1.960 CPU sec, 2.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285007, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 285, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.91, 150.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690352 sequences (100000209 bp) in 1.330 CPU sec, 1.329 real sec...
[M::mem_process_seqs] Processed 686342 reads in 131.268 CPU sec, 4.199 real sec
sorting block 10, #reads = 1074284 mem = 536877715 peak = 891367424
[M::mem_process_seqs] Processed 686108 reads in 81.896 CPU sec, 2.603 real sec
sorting block 11, #reads = 1073569 mem = 536878333 peak = 916373504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284529, 0, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 205, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (240.44, 129.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (311.73, 152.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 184, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (215.92, 106.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689380 sequences (100000184 bp) in 2.072 CPU sec, 2.185 real sec...
[M::process] read 688474 sequences (100000180 bp) in 1.330 CPU sec, 1.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286778, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 273, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (295.21, 144.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685826 reads in 131.762 CPU sec, 4.213 real sec
[M::mem_process_seqs] Processed 690352 reads in 82.539 CPU sec, 2.634 real sec
sorting block 12, #reads = 1072002 mem = 536880548 peak = 916373504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286389, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (299.34, 146.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688258 sequences (100000138 bp) in 1.909 CPU sec, 2.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285903, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 282, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (303.33, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687548 sequences (100000199 bp) in 1.360 CPU sec, 1.352 real sec...
[M::mem_process_seqs] Processed 689380 reads in 131.109 CPU sec, 4.197 real sec
[M::mem_process_seqs] Processed 688474 reads in 81.258 CPU sec, 2.588 real sec
sorting block 13, #reads = 1077136 mem = 536880256 peak = 916373504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285791, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 216, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (217.42, 92.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (305.73, 149.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686472 sequences (100000074 bp) in 2.164 CPU sec, 2.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285273, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 169, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (221.00, 100.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (309.99, 151.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 688258 reads in 124.784 CPU sec, 3.972 real sec
[M::process] read 685520 sequences (100000161 bp) in 1.356 CPU sec, 1.361 real sec...
sorting block 14, #reads = 1075141 mem = 536881129 peak = 916373504
[M::mem_process_seqs] Processed 687548 reads in 79.711 CPU sec, 2.520 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285163, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 189, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (195.08, 71.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (313.68, 152.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 15, #reads = 1074962 mem = 536881477 peak = 916373504
[M::process] read 685992 sequences (100000151 bp) in 1.881 CPU sec, 1.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284769, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (315.90, 152.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685924 sequences (100000003 bp) in 1.360 CPU sec, 1.353 real sec...
[M::mem_process_seqs] Processed 686472 reads in 125.983 CPU sec, 4.014 real sec
[M::mem_process_seqs] Processed 685520 reads in 79.263 CPU sec, 2.511 real sec
sorting block 16, #reads = 1073056 mem = 536879162 peak = 916373504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284803, 0, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 289, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (312.72, 152.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (105, 166, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (177.46, 81.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation RR
[M::process] read 686076 sequences (100000095 bp) in 2.184 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284559, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (311.46, 151.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689248 sequences (100000289 bp) in 1.300 CPU sec, 1.303 real sec...
[M::mem_process_seqs] Processed 685992 reads in 125.696 CPU sec, 3.998 real sec
[M::mem_process_seqs] Processed 685924 reads in 79.860 CPU sec, 2.536 real sec
sorting block 17, #reads = 1072784 mem = 536878499 peak = 916373504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285208, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 289, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (312.44, 152.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689058 sequences (100000013 bp) in 1.973 CPU sec, 2.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286288, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 145, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (179.91, 77.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (297.18, 143.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688366 sequences (100000205 bp) in 1.280 CPU sec, 1.280 real sec...
[M::mem_process_seqs] Processed 686076 reads in 125.590 CPU sec, 4.005 real sec
[M::mem_process_seqs] Processed 689248 reads in 78.585 CPU sec, 2.490 real sec
sorting block 18, #reads = 1072169 mem = 536881572 peak = 916373504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286004, 0, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 266, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (220.06, 78.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 277, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (297.37, 143.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (371, 955, 955)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2123)
[M::mem_pestat] mean and std.dev: (634.67, 342.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2707)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687846 sequences (100000285 bp) in 1.958 CPU sec, 2.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285362, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (300.55, 144.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 169, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (85, 290)
[M::mem_pestat] mean and std.dev: (178.60, 20.71)
[M::mem_pestat] low and high boundaries for proper pairs: (44, 331)
[M::mem_pestat] skip orientation RR
[M::process] read 687740 sequences (100000194 bp) in 1.290 CPU sec, 1.294 real sec...
[M::mem_process_seqs] Processed 689058 reads in 127.016 CPU sec, 4.051 real sec
sorting block 19, #reads = 1075890 mem = 536877093 peak = 916373504
[M::mem_process_seqs] Processed 688366 reads in 80.127 CPU sec, 2.544 real sec
sorting block 20, #reads = 1076050 mem = 536878350 peak = 960679936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285285, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (110, 199, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446)
[M::mem_pestat] mean and std.dev: (179.55, 92.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 558)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (303.58, 146.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686774 sequences (100000253 bp) in 1.912 CPU sec, 2.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285597, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (306.90, 149.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685966 sequences (100000023 bp) in 1.333 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 687846 reads in 128.194 CPU sec, 4.087 real sec
[M::mem_process_seqs] Processed 687740 reads in 80.641 CPU sec, 2.553 real sec
sorting block 21, #reads = 1074339 mem = 536879338 peak = 960679936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285036, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 235, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (211.60, 98.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (312.02, 151.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685856 sequences (100000182 bp) in 2.566 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285134, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 196, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568)
[M::mem_pestat] mean and std.dev: (208.67, 76.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (314.83, 151.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686774 reads in 131.144 CPU sec, 4.197 real sec
[M::mem_process_seqs] Processed 685966 reads in 84.363 CPU sec, 2.667 real sec
[M::process] read 685452 sequences (100000117 bp) in 1.616 CPU sec, 1.683 real sec...
sorting block 22, #reads = 1074904 mem = 536877115 peak = 960679936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284568, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 293, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (314.58, 151.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685776 sequences (100000014 bp) in 2.306 CPU sec, 2.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284605, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (313.54, 151.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685856 reads in 134.739 CPU sec, 4.294 real sec
[M::mem_process_seqs] Processed 685452 reads in 85.301 CPU sec, 2.693 real sec
sorting block 23, #reads = 1073135 mem = 536878396 peak = 960679936
[M::process] read 689388 sequences (100000280 bp) in 2.393 CPU sec, 2.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284477, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (310.74, 150.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1073506 mem = 536878171 peak = 960679936
[M::process] read 691370 sequences (100000284 bp) in 2.444 CPU sec, 2.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286074, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 273, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (297.80, 146.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685776 reads in 136.152 CPU sec, 4.336 real sec
[M::mem_process_seqs] Processed 689388 reads in 86.455 CPU sec, 2.729 real sec
[M::process] read 690664 sequences (100000084 bp) in 2.506 CPU sec, 2.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287290, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 267, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (291.14, 143.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1073187 mem = 536877959 peak = 960679936
[M::process] read 690074 sequences (100000189 bp) in 2.265 CPU sec, 2.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286682, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (92, 123, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434)
[M::mem_pestat] mean and std.dev: (140.91, 49.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 548)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 270, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (295.04, 146.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 691370 reads in 137.043 CPU sec, 4.366 real sec
[M::mem_process_seqs] Processed 690664 reads in 86.192 CPU sec, 2.719 real sec
sorting block 26, #reads = 1075342 mem = 536878604 peak = 960679936
[M::process] read 689434 sequences (100000217 bp) in 1.891 CPU sec, 1.998 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286482, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 174, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (177.92, 60.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 274, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (298.26, 147.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 689092 sequences (100000215 bp) in 1.830 CPU sec, 1.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286805, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (299.38, 147.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690074 reads in 134.598 CPU sec, 4.281 real sec
[M::process] read 687668 sequences (100000065 bp) in 1.290 CPU sec, 1.291 real sec...
[M::mem_process_seqs] Processed 689434 reads in 84.435 CPU sec, 2.668 real sec
sorting block 27, #reads = 1074796 mem = 536876346 peak = 960679936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285852, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (299.04, 146.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686428 sequences (100000244 bp) in 1.878 CPU sec, 1.995 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285739, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (303.03, 147.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686150 sequences (100000293 bp) in 1.480 CPU sec, 1.481 real sec...
[M::mem_process_seqs] Processed 689092 reads in 134.971 CPU sec, 4.295 real sec
sorting block 28, #reads = 1073660 mem = 536878095 peak = 960679936
[M::mem_process_seqs] Processed 687668 reads in 83.996 CPU sec, 2.662 real sec
sorting block 29, #reads = 1072567 mem = 536879285 peak = 1003065344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285042, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.59, 146.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686004 sequences (100000136 bp) in 2.275 CPU sec, 2.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284788, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 176, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356)
[M::mem_pestat] mean and std.dev: (168.82, 63.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 437)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (304.42, 144.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 693516 sequences (100000186 bp) in 1.450 CPU sec, 1.454 real sec...
[M::mem_process_seqs] Processed 686428 reads in 137.458 CPU sec, 4.366 real sec
[M::mem_process_seqs] Processed 686150 reads in 85.925 CPU sec, 2.721 real sec
sorting block 30, #reads = 1070604 mem = 536885658 peak = 1003065344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284554, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (304.51, 144.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 142, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (165.82, 71.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 576)
[M::mem_pestat] skip orientation RR
[M::process] read 692198 sequences (100000040 bp) in 2.086 CPU sec, 2.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288208, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 259, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (283.17, 140.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690890 sequences (100000119 bp) in 1.370 CPU sec, 1.373 real sec...
[M::mem_process_seqs] Processed 686004 reads in 142.042 CPU sec, 4.536 real sec
sorting block 31, #reads = 1069711 mem = 536880219 peak = 1003065344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286856, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 175, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (179.70, 62.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 551)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 264, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (288.03, 141.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 690386 sequences (100000102 bp) in 1.714 CPU sec, 1.722 real sec...
[M::mem_process_seqs] Processed 692198 reads in 76.257 CPU sec, 2.413 real sec
[M::mem_process_seqs] Processed 693516 reads in 190.532 CPU sec, 6.057 real sec
sorting block 32, #reads = 1073744 mem = 536880243 peak = 1003065344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287500, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 270, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (292.48, 142.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689672 sequences (100000209 bp) in 1.912 CPU sec, 2.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286867, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 273, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (294.74, 143.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 252, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (217.00, 84.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RR
[M::process] read 688886 sequences (100000170 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_process_seqs] Processed 690890 reads in 149.599 CPU sec, 4.756 real sec
[M::mem_process_seqs] Processed 690386 reads in 86.104 CPU sec, 2.727 real sec
sorting block 33, #reads = 1074752 mem = 536881297 peak = 1003065344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 286473, 0, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 193, 1330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3702)
[M::mem_pestat] mean and std.dev: (542.12, 488.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4888)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 275, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (296.69, 144.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 188, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (166.00, 74.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 764)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687194 sequences (100000138 bp) in 1.918 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286554, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 124, 154)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (40, 230)
[M::mem_pestat] mean and std.dev: (128.70, 21.34)
[M::mem_pestat] low and high boundaries for proper pairs: (2, 268)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 276, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (298.26, 144.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 209, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (83, 378)
[M::mem_pestat] mean and std.dev: (219.25, 44.34)
[M::mem_pestat] low and high boundaries for proper pairs: (24, 437)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686874 sequences (100000041 bp) in 1.346 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 689672 reads in 135.914 CPU sec, 4.332 real sec
[M::mem_process_seqs] Processed 688886 reads in 82.910 CPU sec, 2.625 real sec
sorting block 34, #reads = 1074099 mem = 536878243 peak = 1003065344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285600, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (305.02, 146.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1073537 mem = 536877744 peak = 1003065344
[M::process] read 686556 sequences (100000288 bp) in 1.875 CPU sec, 1.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285239, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (306.08, 147.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 166, 192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 268)
[M::mem_pestat] mean and std.dev: (159.20, 38.26)
[M::mem_pestat] low and high boundaries for proper pairs: (6, 312)
[M::mem_pestat] skip orientation RR
[M::process] read 688892 sequences (100000177 bp) in 1.430 CPU sec, 1.424 real sec...
[M::mem_process_seqs] Processed 687194 reads in 133.335 CPU sec, 4.243 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284887, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 285, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (306.47, 147.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691986 sequences (100000211 bp) in 1.722 CPU sec, 1.729 real sec...
[M::mem_process_seqs] Processed 686556 reads in 73.631 CPU sec, 2.331 real sec
sorting block 36, #reads = 1072666 mem = 536879397 peak = 1003065344
[M::mem_process_seqs] Processed 686874 reads in 183.437 CPU sec, 5.822 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286015, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 275, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (297.73, 145.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690538 sequences (100000008 bp) in 1.917 CPU sec, 2.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287347, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 265, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (287.37, 140.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689710 sequences (100000219 bp) in 1.330 CPU sec, 1.326 real sec...
[M::mem_process_seqs] Processed 688892 reads in 152.502 CPU sec, 4.841 real sec
sorting block 37, #reads = 1069658 mem = 536879231 peak = 1003065344
[M::mem_process_seqs] Processed 691986 reads in 84.980 CPU sec, 2.695 real sec
sorting block 38, #reads = 1071439 mem = 536879035 peak = 1047285760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286879, 0, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 380)
[M::mem_pestat] mean and std.dev: (204.50, 76.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 511)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 271, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (292.07, 140.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 208, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181)
[M::mem_pestat] mean and std.dev: (300.36, 162.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1516)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689148 sequences (100000102 bp) in 2.445 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286562, 0, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 275, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (295.41, 142.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 237, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (199.73, 81.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690538 reads in 132.881 CPU sec, 4.217 real sec
[M::process] read 688352 sequences (100000223 bp) in 1.380 CPU sec, 1.378 real sec...
[M::mem_process_seqs] Processed 689710 reads in 83.580 CPU sec, 2.642 real sec
sorting block 39, #reads = 1074875 mem = 536879853 peak = 1047285760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286217, 0, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (296.53, 142.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 194, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (172, 222)
[M::mem_pestat] mean and std.dev: (197.60, 10.14)
[M::mem_pestat] low and high boundaries for proper pairs: (157, 238)
[M::mem_pestat] skip orientation RR
[M::process] read 688130 sequences (100000295 bp) in 1.872 CPU sec, 1.963 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286270, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 279, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (299.29, 143.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 316, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (260.08, 75.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 680)
[M::mem_pestat] skip orientation RR
[M::process] read 687480 sequences (100000191 bp) in 1.360 CPU sec, 1.369 real sec...
[M::mem_process_seqs] Processed 689148 reads in 132.164 CPU sec, 4.208 real sec
[M::mem_process_seqs] Processed 688352 reads in 82.401 CPU sec, 2.614 real sec
sorting block 40, #reads = 1073835 mem = 536878672 peak = 1047285760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285796, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 280, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.59, 142.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687166 sequences (100000027 bp) in 2.189 CPU sec, 2.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285850, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 282, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (302.59, 144.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 262, 1011)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2717)
[M::mem_pestat] mean and std.dev: (472.90, 421.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3570)
[M::mem_pestat] skip orientation RR
[M::process] read 687112 sequences (100000168 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 688130 reads in 134.191 CPU sec, 4.262 real sec
sorting block 41, #reads = 1073069 mem = 536878848 peak = 1047285760
[M::mem_process_seqs] Processed 687480 reads in 83.304 CPU sec, 2.639 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286048, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 187, 803)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2043)
[M::mem_pestat] mean and std.dev: (345.64, 312.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2663)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (304.07, 145.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 193, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (179.50, 55.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 749)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 42, #reads = 1071396 mem = 536876727 peak = 1047285760
[M::process] read 690776 sequences (100000050 bp) in 1.998 CPU sec, 2.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285735, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (304.75, 146.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 246, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (254.50, 64.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 707)
[M::mem_pestat] skip orientation RR
[M::process] read 692600 sequences (100000104 bp) in 1.380 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 687166 reads in 135.452 CPU sec, 4.308 real sec
[M::mem_process_seqs] Processed 687112 reads in 84.996 CPU sec, 2.690 real sec
sorting block 43, #reads = 1071238 mem = 536878164 peak = 1047285760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286651, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 134, 150)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (87, 192)
[M::mem_pestat] mean and std.dev: (140.20, 22.05)
[M::mem_pestat] low and high boundaries for proper pairs: (52, 228)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 268, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (292.48, 144.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 690472 sequences (100000037 bp) in 2.169 CPU sec, 2.257 real sec...
[M::process] read 690268 sequences (100000201 bp) in 1.350 CPU sec, 1.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287714, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 264, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (287.91, 141.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690776 reads in 139.747 CPU sec, 4.439 real sec
[M::mem_process_seqs] Processed 692600 reads in 85.365 CPU sec, 2.709 real sec
sorting block 44, #reads = 1069979 mem = 536878667 peak = 1047285760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287266, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 271, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (293.68, 142.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689366 sequences (100000146 bp) in 1.935 CPU sec, 2.027 real sec...
[M::process] read 688792 sequences (100000274 bp) in 1.350 CPU sec, 1.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287036, 6, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 208, 2841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8205)
[M::mem_pestat] mean and std.dev: (912.09, 1182.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10887)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 274, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (296.37, 144.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 199, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (189.21, 58.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 456)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690472 reads in 134.626 CPU sec, 4.285 real sec
[M::mem_process_seqs] Processed 690268 reads in 84.371 CPU sec, 2.673 real sec
sorting block 45, #reads = 1074845 mem = 536878651 peak = 1047285760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286434, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (299.26, 145.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1073208 mem = 536877713 peak = 1047285760
[M::process] read 688770 sequences (100000089 bp) in 2.180 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286357, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 279, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (300.88, 145.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687628 sequences (100000220 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_process_seqs] Processed 689366 reads in 133.326 CPU sec, 4.236 real sec
[M::mem_process_seqs] Processed 688792 reads in 83.539 CPU sec, 2.641 real sec
sorting block 47, #reads = 1073232 mem = 536883959 peak = 1047285760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286176, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 279, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (299.88, 144.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687078 sequences (100000119 bp) in 1.902 CPU sec, 1.991 real sec...
[M::process] read 686116 sequences (100000231 bp) in 1.350 CPU sec, 1.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 285561, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 201, 1086)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2962)
[M::mem_pestat] mean and std.dev: (399.71, 390.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3900)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (301.47, 143.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 688770 reads in 133.848 CPU sec, 4.260 real sec
[M::mem_process_seqs] Processed 687628 reads in 82.631 CPU sec, 2.618 real sec
sorting block 48, #reads = 1073088 mem = 536878928 peak = 1047285760
[M::process] read 73572 sequences (10708791 bp) in 0.442 CPU sec, 0.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285896, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 198, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (199.45, 87.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (303.22, 144.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 30692, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (300.41, 142.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284817, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (304.75, 144.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 287, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (307.30, 86.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687078 reads in 137.504 CPU sec, 4.456 real sec
[M::mem_process_seqs] Processed 73572 reads in 78.672 CPU sec, 2.537 real sec
[M::mem_process_seqs] Processed 686116 reads in 90.592 CPU sec, 2.944 real sec
sorting block 49, #reads = 1071892 mem = 536878870 peak = 1047285760
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 54443994
overall: 69808939008 mem 7015.936 user 282.815 sys 963.620 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0001_B23KGCJLT4_1.L008\tPU:L008\tSM:NA12878_c_0002_gDNA_0001_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0001_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0001_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0001_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 963.668 sec; CPU: 7298.767 sec
sorting block 50, #reads = 1031961 mem = 517208109 peak = 1136300032
uniq header count 1
merging 51 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2799638013
output reads: 54707488
bam_mem_sort: 51 calls 22.266 user 0.311 sys 22.710 real
bam_write: 51 calls 14.274 user 0.506 sys 14.932 real
execute: 1 calls 10.390 user 0.566 sys 979.380 real
merge_files: 1 calls 10.370 user 0.547 sys 14.625 real
parse_chunk: 816 calls 29.603 user 7.841 sys 37.905 real
read_chunk: 2205 calls 18.643 user 16.406 sys 189.409 real
sort_block: 1 calls 15.751 user 15.908 sys 184.259 real
write_chunk: 1387 calls 10.021 user 0.406 sys 13.927 real
overall: 2507685888 mem 575.899 user 33.760 sys 979.398 real
ls: cannot access '*.cram': No such file or directory