.command.err - Workdir - DAQ:SENTIEON_BWAMEM (NA12878_c_0002_gDNA_0002_B23KGCJLT4_1;L005;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bb/bca0d4399f7cf94e3421e63aa5b2a7/.command.err
Size: 76.5 KB
Task: DAQ:SENTIEON_BWAMEM (NA12878_c_0002_gDNA_0002_B23KGCJLT4_1;L005;0001)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0002_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1064.674 CPU sec, 94.452 real sec
[M::process] read 688374 sequences (100000238 bp) in 0.853 CPU sec, 0.856 real sec...
[M::process] read 686952 sequences (100000033 bp) in 1.020 CPU sec, 1.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285353, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 137, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389)
[M::mem_pestat] mean and std.dev: (137.38, 36.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (306.04, 146.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686398 sequences (100000265 bp) in 1.090 CPU sec, 1.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285129, 7, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (313.25, 149.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 237, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (267.07, 109.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RR
[M::process] read 687422 sequences (100000159 bp) in 1.160 CPU sec, 1.154 real sec...
[M::mem_process_seqs] Processed 688374 reads in 105.408 CPU sec, 3.298 real sec
[M::mem_process_seqs] Processed 686952 reads in 69.894 CPU sec, 2.204 real sec
sorting block 0, #reads = 1072664 mem = 536879188 peak = 595902464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284978, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 260, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436)
[M::mem_pestat] mean and std.dev: (216.13, 53.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 524)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (318.37, 153.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687584 sequences (100000169 bp) in 1.450 CPU sec, 1.493 real sec...
[M::process] read 687264 sequences (100000254 bp) in 1.160 CPU sec, 1.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285531, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 136, 4061)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11911)
[M::mem_pestat] mean and std.dev: (1118.25, 1699.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (314.48, 152.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686398 reads in 104.290 CPU sec, 3.296 real sec
[M::mem_process_seqs] Processed 687422 reads in 68.772 CPU sec, 2.183 real sec
sorting block 1, #reads = 1073714 mem = 536881101 peak = 726020096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284840, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 687034 sequences (100000147 bp) in 1.443 CPU sec, 1.478 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (311.75, 150.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686448 sequences (100000282 bp) in 1.180 CPU sec, 1.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284803, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (311.58, 149.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687584 reads in 106.434 CPU sec, 3.366 real sec
sorting block 2, #reads = 1074506 mem = 536879837 peak = 892612608
[M::mem_process_seqs] Processed 687264 reads in 69.202 CPU sec, 2.194 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285680, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (312.80, 150.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686328 sequences (100000234 bp) in 1.677 CPU sec, 1.721 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284769, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 686464 sequences (100000034 bp) in 1.210 CPU sec, 1.211 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (314.76, 151.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687034 reads in 106.570 CPU sec, 3.367 real sec
sorting block 3, #reads = 1074196 mem = 536878264 peak = 962560000
[M::mem_process_seqs] Processed 686448 reads in 69.820 CPU sec, 2.211 real sec
sorting block 4, #reads = 1072844 mem = 536877861 peak = 996372480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284466, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (312.04, 150.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687930 sequences (100000146 bp) in 1.518 CPU sec, 1.574 real sec...
[M::process] read 687138 sequences (100000211 bp) in 1.190 CPU sec, 1.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284522, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (287, 301, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (223, 383)
[M::mem_pestat] mean and std.dev: (299.33, 10.80)
[M::mem_pestat] low and high boundaries for proper pairs: (191, 415)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (312.76, 151.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (98, 144, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (146.75, 72.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686328 reads in 107.735 CPU sec, 3.413 real sec
[M::mem_process_seqs] Processed 686464 reads in 71.628 CPU sec, 2.265 real sec
sorting block 5, #reads = 1072539 mem = 536879517 peak = 996372480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285538, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 256, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 622)
[M::mem_pestat] mean and std.dev: (255.07, 97.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (311.85, 149.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686270 sequences (100000232 bp) in 1.712 CPU sec, 1.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285050, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 807, 807)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2123)
[M::mem_pestat] mean and std.dev: (511.81, 314.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2781)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 297, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (315.69, 151.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687090 sequences (100000077 bp) in 1.240 CPU sec, 1.242 real sec...
[M::mem_process_seqs] Processed 687930 reads in 105.497 CPU sec, 3.330 real sec
[M::mem_process_seqs] Processed 687138 reads in 69.713 CPU sec, 2.204 real sec
sorting block 6, #reads = 1072933 mem = 536880137 peak = 996372480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284803, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 302, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (320.46, 153.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688254 sequences (100000271 bp) in 1.594 CPU sec, 1.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285451, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (108, 134, 167)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285)
[M::mem_pestat] mean and std.dev: (124.50, 21.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 344)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (316.43, 153.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687422 sequences (100000049 bp) in 1.250 CPU sec, 1.248 real sec...
[M::mem_process_seqs] Processed 686270 reads in 107.073 CPU sec, 3.387 real sec
sorting block 7, #reads = 1073932 mem = 536877960 peak = 996372480
[M::mem_process_seqs] Processed 687090 reads in 70.542 CPU sec, 2.222 real sec
sorting block 8, #reads = 1073478 mem = 536879284 peak = 996372480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285642, 1, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 167, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (199.00, 96.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (308.62, 149.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 217, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (233.63, 116.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686840 sequences (100000041 bp) in 1.810 CPU sec, 1.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284963, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 193, 783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983)
[M::mem_pestat] mean and std.dev: (322.08, 268.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2583)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (312.48, 151.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686968 sequences (100000093 bp) in 1.310 CPU sec, 1.308 real sec...
[M::mem_process_seqs] Processed 688254 reads in 108.186 CPU sec, 3.416 real sec
[M::mem_process_seqs] Processed 687422 reads in 71.266 CPU sec, 2.246 real sec
sorting block 9, #reads = 1074556 mem = 536880036 peak = 996372480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284656, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (312.47, 150.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686984 sequences (100000120 bp) in 1.638 CPU sec, 1.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285021, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (309.73, 149.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687602 sequences (100000113 bp) in 1.330 CPU sec, 1.332 real sec...
[M::mem_process_seqs] Processed 686840 reads in 107.895 CPU sec, 3.411 real sec
[M::mem_process_seqs] Processed 686968 reads in 71.046 CPU sec, 2.244 real sec
sorting block 10, #reads = 1073483 mem = 536878928 peak = 996372480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285251, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (309.98, 150.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687310 sequences (100000244 bp) in 1.886 CPU sec, 1.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285098, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 289, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (310.30, 150.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 245, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (255.36, 109.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RR
[M::process] read 686236 sequences (100000205 bp) in 1.280 CPU sec, 1.282 real sec...
[M::mem_process_seqs] Processed 686984 reads in 108.544 CPU sec, 3.427 real sec
sorting block 11, #reads = 1073067 mem = 536879482 peak = 996372480
[M::mem_process_seqs] Processed 687602 reads in 71.644 CPU sec, 2.260 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284884, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 583, 840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2160)
[M::mem_pestat] mean and std.dev: (516.14, 304.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2820)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (314.06, 150.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685544 sequences (100000085 bp) in 1.705 CPU sec, 1.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284429, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 301, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (319.25, 152.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 261, 1836)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4986)
[M::mem_pestat] mean and std.dev: (661.09, 723.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6561)
[M::mem_pestat] skip orientation RR
[M::process] read 686682 sequences (100000024 bp) in 1.290 CPU sec, 1.286 real sec...
[M::mem_process_seqs] Processed 687310 reads in 106.567 CPU sec, 3.369 real sec
sorting block 12, #reads = 1072508 mem = 536880954 peak = 996372480
[M::mem_process_seqs] Processed 686236 reads in 70.120 CPU sec, 2.213 real sec
sorting block 13, #reads = 1073445 mem = 536878300 peak = 1022668800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284425, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 304, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (323.91, 155.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 177, 3053)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8923)
[M::mem_pestat] mean and std.dev: (1020.60, 1331.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11858)
[M::mem_pestat] skip orientation RR
[M::process] read 687300 sequences (100000085 bp) in 1.945 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284650, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 296, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (315.75, 151.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 240, 1443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4069)
[M::mem_pestat] mean and std.dev: (747.17, 949.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5382)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685544 reads in 107.637 CPU sec, 3.398 real sec
[M::process] read 686306 sequences (100000065 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285330, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 192, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354)
[M::mem_pestat] mean and std.dev: (167.30, 41.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 427)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (313.01, 151.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686494 sequences (100000161 bp) in 1.349 CPU sec, 1.354 real sec...
[M::mem_process_seqs] Processed 687300 reads in 58.515 CPU sec, 1.847 real sec
sorting block 14, #reads = 1072382 mem = 536878470 peak = 1022668800
[M::mem_process_seqs] Processed 686682 reads in 144.332 CPU sec, 4.563 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284465, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (316.24, 152.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 216, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (198.45, 68.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 659)
[M::mem_pestat] skip orientation RR
sorting block 15, #reads = 1073030 mem = 536880464 peak = 1022668800
[M::process] read 687010 sequences (100000042 bp) in 1.948 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285017, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (312.77, 150.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686306 reads in 119.280 CPU sec, 3.776 real sec
[M::mem_process_seqs] Processed 686494 reads in 71.897 CPU sec, 2.261 real sec
[M::process] read 686410 sequences (100000227 bp) in 1.534 CPU sec, 1.557 real sec...
sorting block 16, #reads = 1072412 mem = 536881062 peak = 1022668800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284993, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (310.06, 150.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687506 sequences (100000274 bp) in 1.804 CPU sec, 1.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284496, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 202, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (225.14, 95.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 292, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (314.05, 152.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 687010 reads in 107.628 CPU sec, 3.404 real sec
[M::process] read 686864 sequences (100000158 bp) in 1.287 CPU sec, 1.293 real sec...
[M::mem_process_seqs] Processed 686410 reads in 71.040 CPU sec, 2.231 real sec
sorting block 17, #reads = 1072711 mem = 536878317 peak = 1022668800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285150, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (309.82, 150.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685746 sequences (100000145 bp) in 1.924 CPU sec, 1.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285292, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 207, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (99, 319)
[M::mem_pestat] mean and std.dev: (190.10, 45.32)
[M::mem_pestat] low and high boundaries for proper pairs: (9, 371)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 295, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (314.28, 151.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 687506 reads in 107.017 CPU sec, 3.383 real sec
[M::process] read 685196 sequences (100000031 bp) in 1.308 CPU sec, 1.316 real sec...
sorting block 18, #reads = 1073208 mem = 536880043 peak = 1022668800
[M::mem_process_seqs] Processed 686864 reads in 69.860 CPU sec, 2.199 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285074, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 300, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (319.37, 153.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687022 sequences (100000118 bp) in 1.915 CPU sec, 1.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284610, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 303, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (323.11, 155.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685746 reads in 106.706 CPU sec, 3.371 real sec
[M::process] read 687184 sequences (100000025 bp) in 1.300 CPU sec, 1.295 real sec...
sorting block 19, #reads = 1073110 mem = 536878701 peak = 1022668800
[M::mem_process_seqs] Processed 685196 reads in 69.950 CPU sec, 2.201 real sec
sorting block 20, #reads = 1072534 mem = 536879721 peak = 1087631360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285168, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 292, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (314.00, 152.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686656 sequences (100000091 bp) in 1.914 CPU sec, 1.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285315, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (311.73, 150.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687022 reads in 107.257 CPU sec, 3.387 real sec
[M::process] read 687208 sequences (100000080 bp) in 1.349 CPU sec, 1.347 real sec...
[M::mem_process_seqs] Processed 687184 reads in 70.794 CPU sec, 2.225 real sec
sorting block 21, #reads = 1073850 mem = 536877620 peak = 1087631360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285207, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (311.64, 150.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687086 sequences (100000116 bp) in 1.937 CPU sec, 1.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284363, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 285, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (307.23, 149.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686656 reads in 107.479 CPU sec, 3.399 real sec
[M::process] read 686666 sequences (100000243 bp) in 1.366 CPU sec, 1.373 real sec...
[M::mem_process_seqs] Processed 687208 reads in 71.662 CPU sec, 2.255 real sec
sorting block 22, #reads = 1073657 mem = 536880879 peak = 1087631360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285037, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (309.55, 150.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 226, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (276.70, 125.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RR
[M::process] read 689860 sequences (100000099 bp) in 1.854 CPU sec, 1.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284861, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 288, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (311.05, 151.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687086 reads in 108.201 CPU sec, 3.421 real sec
[M::process] read 688004 sequences (100000090 bp) in 1.348 CPU sec, 1.356 real sec...
sorting block 23, #reads = 1072559 mem = 536880556 peak = 1087631360
[M::mem_process_seqs] Processed 686666 reads in 71.657 CPU sec, 2.258 real sec
sorting block 24, #reads = 1072351 mem = 536877605 peak = 1087631360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286490, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (302.93, 148.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687264 sequences (100000004 bp) in 1.950 CPU sec, 2.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285756, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 141, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (169.20, 61.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 654)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (312.85, 152.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688136 sequences (100000136 bp) in 1.330 CPU sec, 1.323 real sec...
[M::mem_process_seqs] Processed 689860 reads in 112.552 CPU sec, 3.556 real sec
[M::mem_process_seqs] Processed 688004 reads in 72.471 CPU sec, 2.284 real sec
sorting block 25, #reads = 1074466 mem = 536877927 peak = 1087631360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285377, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 295, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (316.09, 153.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 165, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (188.64, 68.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 618)
[M::mem_pestat] skip orientation RR
[M::process] read 688752 sequences (100000158 bp) in 1.956 CPU sec, 2.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286068, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (311.24, 151.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688278 sequences (100000064 bp) in 1.340 CPU sec, 1.340 real sec...
[M::mem_process_seqs] Processed 687264 reads in 111.362 CPU sec, 3.516 real sec
[M::mem_process_seqs] Processed 688136 reads in 75.201 CPU sec, 2.372 real sec
sorting block 26, #reads = 1072355 mem = 536881097 peak = 1087631360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286217, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (306.97, 149.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687766 sequences (100000141 bp) in 2.009 CPU sec, 2.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285665, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 687788 sequences (100000060 bp) in 1.470 CPU sec, 1.472 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.26, 144.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688752 reads in 137.075 CPU sec, 4.343 real sec
sorting block 27, #reads = 1072741 mem = 536879127 peak = 1087631360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285732, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (305.73, 145.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687302 sequences (100000270 bp) in 2.270 CPU sec, 2.275 real sec...
[M::mem_process_seqs] Processed 687766 reads in 79.105 CPU sec, 2.483 real sec
[M::mem_process_seqs] Processed 688278 reads in 186.854 CPU sec, 5.906 real sec
sorting block 28, #reads = 1073474 mem = 536880111 peak = 1087631360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285439, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (303.29, 143.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688096 sequences (100000048 bp) in 2.794 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285710, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (305.54, 144.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 168, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150)
[M::mem_pestat] mean and std.dev: (266.92, 172.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1487)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687788 reads in 159.492 CPU sec, 5.034 real sec
[M::mem_process_seqs] Processed 687302 reads in 94.992 CPU sec, 2.977 real sec
sorting block 29, #reads = 1072635 mem = 536878872 peak = 1087631360
[M::process] read 689242 sequences (100000081 bp) in 2.470 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285620, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 370, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (247.82, 116.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 286, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (306.17, 146.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687550 sequences (100000013 bp) in 2.423 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285801, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 286, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (307.22, 149.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688096 reads in 147.647 CPU sec, 4.674 real sec
sorting block 30, #reads = 1071519 mem = 536876695 peak = 1087631360
[M::mem_process_seqs] Processed 689242 reads in 96.881 CPU sec, 3.041 real sec
[M::process] read 686852 sequences (100000146 bp) in 2.346 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285516, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 245, 969)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2495)
[M::mem_pestat] mean and std.dev: (436.75, 372.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3258)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (312.87, 150.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 31, #reads = 1071279 mem = 536878163 peak = 1087631360
[M::process] read 688512 sequences (100000158 bp) in 2.641 CPU sec, 2.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285512, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 176, 187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (109, 239)
[M::mem_pestat] mean and std.dev: (170.75, 11.50)
[M::mem_pestat] low and high boundaries for proper pairs: (83, 265)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (314.51, 150.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 687550 reads in 144.111 CPU sec, 4.564 real sec
[M::mem_process_seqs] Processed 686852 reads in 95.901 CPU sec, 3.010 real sec
[M::process] read 688756 sequences (100000132 bp) in 2.490 CPU sec, 2.522 real sec...
sorting block 32, #reads = 1071785 mem = 536880842 peak = 1087631360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286165, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (305.12, 147.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 263, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (256.00, 52.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] skip orientation RR
[M::process] read 687804 sequences (100000274 bp) in 2.409 CPU sec, 2.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286486, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (302.56, 144.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 172, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (258.42, 151.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1320)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688512 reads in 145.353 CPU sec, 4.604 real sec
[M::mem_process_seqs] Processed 688756 reads in 95.529 CPU sec, 2.997 real sec
sorting block 33, #reads = 1071449 mem = 536878679 peak = 1087631360
[M::process] read 687442 sequences (100000011 bp) in 2.413 CPU sec, 2.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 285391, 0, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 164, 183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (126, 221)
[M::mem_pestat] mean and std.dev: (167.20, 16.66)
[M::mem_pestat] low and high boundaries for proper pairs: (101, 240)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 285, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.08, 145.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 230, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (54, 369)
[M::mem_pestat] mean and std.dev: (210.67, 50.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 432)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687476 sequences (100000108 bp) in 2.545 CPU sec, 2.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285322, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 196, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (205.50, 100.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (306.49, 145.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 687804 reads in 144.718 CPU sec, 4.579 real sec
sorting block 34, #reads = 1073059 mem = 536880825 peak = 1087631360
[M::mem_process_seqs] Processed 687442 reads in 95.881 CPU sec, 3.010 real sec
[M::process] read 687418 sequences (100000243 bp) in 2.422 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285156, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 115, 191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (136.75, 42.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 458)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (306.93, 146.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 35, #reads = 1072679 mem = 536878447 peak = 1095147520
[M::process] read 688928 sequences (100000082 bp) in 2.459 CPU sec, 2.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285649, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (309.65, 149.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687476 reads in 148.261 CPU sec, 4.680 real sec
[M::mem_process_seqs] Processed 687418 reads in 94.981 CPU sec, 2.972 real sec
[M::process] read 687052 sequences (100000049 bp) in 1.945 CPU sec, 1.972 real sec...
sorting block 36, #reads = 1071791 mem = 536878334 peak = 1095147520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285667, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (306.34, 148.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685540 sequences (100000200 bp) in 1.966 CPU sec, 2.015 real sec...
[M::process] read 685504 sequences (100000014 bp) in 1.380 CPU sec, 1.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285364, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (312.12, 148.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688928 reads in 137.324 CPU sec, 4.355 real sec
[M::mem_process_seqs] Processed 687052 reads in 90.608 CPU sec, 2.869 real sec
sorting block 37, #reads = 1069807 mem = 536879698 peak = 1095147520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284659, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (317.36, 149.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687856 sequences (100000093 bp) in 2.299 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285257, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (317.07, 149.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685540 reads in 130.593 CPU sec, 4.128 real sec
[M::mem_process_seqs] Processed 685504 reads in 87.355 CPU sec, 2.737 real sec
[M::process] read 687774 sequences (100000020 bp) in 1.951 CPU sec, 1.976 real sec...
sorting block 38, #reads = 1072032 mem = 536877541 peak = 1095147520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285474, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (306.12, 145.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687098 sequences (100000091 bp) in 1.940 CPU sec, 1.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285359, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 286, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (304.64, 144.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 270, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (117, 372)
[M::mem_pestat] mean and std.dev: (238.44, 47.05)
[M::mem_pestat] low and high boundaries for proper pairs: (50, 427)
[M::mem_pestat] skip orientation RR
[M::process] read 687144 sequences (100000171 bp) in 1.430 CPU sec, 1.434 real sec...
[M::mem_process_seqs] Processed 687856 reads in 134.193 CPU sec, 4.252 real sec
sorting block 39, #reads = 1071467 mem = 536878334 peak = 1095147520
[M::mem_process_seqs] Processed 687774 reads in 88.629 CPU sec, 2.799 real sec
sorting block 40, #reads = 1072878 mem = 536877895 peak = 1108365312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285348, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (306.20, 144.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686904 sequences (100000044 bp) in 2.148 CPU sec, 2.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285806, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (307.37, 145.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 240, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (208.00, 74.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 732)
[M::mem_pestat] skip orientation RR
[M::process] read 687266 sequences (100000051 bp) in 1.500 CPU sec, 1.497 real sec...
[M::mem_process_seqs] Processed 687098 reads in 137.972 CPU sec, 4.369 real sec
[M::mem_process_seqs] Processed 687144 reads in 92.174 CPU sec, 2.907 real sec
sorting block 41, #reads = 1072281 mem = 536883428 peak = 1108365312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285101, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (307.22, 146.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689492 sequences (100000180 bp) in 2.580 CPU sec, 2.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285096, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (308.65, 148.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686904 reads in 146.131 CPU sec, 4.603 real sec
[M::process] read 687490 sequences (100000196 bp) in 1.969 CPU sec, 1.965 real sec...
sorting block 42, #reads = 1070928 mem = 536878928 peak = 1108365312
[M::mem_process_seqs] Processed 687266 reads in 100.780 CPU sec, 3.165 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286281, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (306.43, 151.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 168, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (191.50, 95.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RR
[M::process] read 686398 sequences (100000170 bp) in 2.122 CPU sec, 2.164 real sec...
[M::process] read 686962 sequences (100000241 bp) in 1.470 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285658, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 294, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (314.73, 152.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689492 reads in 141.185 CPU sec, 4.465 real sec
sorting block 43, #reads = 1069203 mem = 536878875 peak = 1108365312
[M::mem_process_seqs] Processed 687490 reads in 92.042 CPU sec, 2.920 real sec
sorting block 44, #reads = 1072004 mem = 536875283 peak = 1108365312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285363, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 301, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (321.45, 156.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 179, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102)
[M::mem_pestat] mean and std.dev: (196.20, 97.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1416)
[M::mem_pestat] skip orientation RR
[M::process] read 688108 sequences (100000190 bp) in 2.630 CPU sec, 2.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285838, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 295, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (316.22, 153.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686398 reads in 133.679 CPU sec, 4.210 real sec
[M::process] read 687024 sequences (100000277 bp) in 1.960 CPU sec, 1.972 real sec...
[M::mem_process_seqs] Processed 686962 reads in 89.302 CPU sec, 2.792 real sec
sorting block 45, #reads = 1071419 mem = 536877195 peak = 1108365312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285544, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 287, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (308.29, 149.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687224 sequences (100000280 bp) in 1.921 CPU sec, 1.962 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285145, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (310.68, 149.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686988 sequences (100000155 bp) in 1.450 CPU sec, 1.455 real sec...
[M::mem_process_seqs] Processed 688108 reads in 134.479 CPU sec, 4.268 real sec
[M::mem_process_seqs] Processed 687024 reads in 90.386 CPU sec, 2.857 real sec
sorting block 46, #reads = 1072806 mem = 536878816 peak = 1108365312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285034, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 286, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.71, 149.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 198, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (217.00, 101.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RR
[M::process] read 686776 sequences (100000144 bp) in 2.671 CPU sec, 2.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284959, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (307.93, 147.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 416894 sequences (60688284 bp) in 1.280 CPU sec, 1.284 real sec...
[M::mem_process_seqs] Processed 687224 reads in 138.512 CPU sec, 4.375 real sec
sorting block 47, #reads = 1071470 mem = 536878520 peak = 1108365312
[M::mem_process_seqs] Processed 686988 reads in 92.051 CPU sec, 2.906 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285011, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (307.33, 147.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 172674, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 288, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (309.92, 149.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 291, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141)
[M::mem_pestat] mean and std.dev: (311.00, 147.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1463)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686776 reads in 113.793 CPU sec, 3.685 real sec
sorting block 48, #reads = 1071541 mem = 536878057 peak = 1189797888
[M::mem_process_seqs] Processed 416894 reads in 63.594 CPU sec, 2.057 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 53335912
overall: 69643890688 mem 6252.125 user 81.683 sys 267.671 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0002_B23KGCJLT4_1.L005\tPU:L005\tSM:NA12878_c_0002_gDNA_0002_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0002_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 267.702 sec; CPU: 6333.811 sec
sorting block 49, #reads = 1041433 mem = 522410646 peak = 1230508032
uniq header count 1
merging 50 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 96
algo: util-sort
license: sentieon:util=1
output file size: 2792858861
output reads: 53598170
bam_mem_sort: 50 calls 21.773 user 0.253 sys 22.158 real
bam_write: 50 calls 27.019 user 0.764 sys 27.907 real
execute: 1 calls 15.286 user 0.427 sys 288.145 real
merge_files: 1 calls 15.270 user 0.405 sys 19.487 real
parse_chunk: 800 calls 39.609 user 6.001 sys 46.194 real
read_chunk: 2162 calls 15.109 user 13.067 sys 176.244 real
sort_block: 1 calls 10.897 user 12.821 sys 170.031 real
write_chunk: 1360 calls 14.899 user 0.257 sys 18.799 real
overall: 2575802368 mem 747.477 user 24.290 sys 288.164 real
ls: cannot access '*.cram': No such file or directory