cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_rd-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1671.559 CPU sec, 117.723 real sec
[M::process] read 687046 sequences (100000082 bp) in 0.910 CPU sec, 0.914 real sec...
[M::process] read 685724 sequences (100000081 bp) in 1.150 CPU sec, 1.164 real sec...
[M::process] read 685116 sequences (100000006 bp) in 1.192 CPU sec, 1.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283236, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (300.14, 146.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684678 sequences (100000108 bp) in 1.131 CPU sec, 1.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282922, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 286, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (309.09, 151.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283165, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (306.05, 149.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687046 reads in 205.669 CPU sec, 6.631 real sec
[M::mem_process_seqs] Processed 685116 reads in 135.713 CPU sec, 4.431 real sec
[M::mem_process_seqs] Processed 685724 reads in 147.215 CPU sec, 4.818 real sec
sorting block 0, #reads = 1089064 mem = 536879694 peak = 586563584
[M::process] read 684200 sequences (100000034 bp) in 1.705 CPU sec, 1.861 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282873, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (310.80, 151.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684048 sequences (100000165 bp) in 1.309 CPU sec, 1.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282723, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 290, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (315.04, 154.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684678 reads in 124.210 CPU sec, 3.949 real sec
sorting block 1, #reads = 1088658 mem = 536881488 peak = 801193984
[M::mem_process_seqs] Processed 684200 reads in 79.219 CPU sec, 2.492 real sec
[M::process] read 684384 sequences (100000133 bp) in 1.326 CPU sec, 1.356 real sec...
sorting block 2, #reads = 1088658 mem = 536879769 peak = 842428416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282444, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 293, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (316.50, 155.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682772 sequences (100000168 bp) in 1.534 CPU sec, 1.655 real sec...
[M::process] read 682388 sequences (100000034 bp) in 1.170 CPU sec, 1.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282520, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (314.56, 154.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684048 reads in 124.557 CPU sec, 3.968 real sec
[M::mem_process_seqs] Processed 684384 reads in 78.668 CPU sec, 2.501 real sec
sorting block 3, #reads = 1087981 mem = 536881244 peak = 842428416
[M::process] read 682446 sequences (100000294 bp) in 1.694 CPU sec, 1.785 real sec...
[M::process] read 682060 sequences (100000007 bp) in 1.160 CPU sec, 1.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282007, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (319.05, 154.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682388 reads in 67.206 CPU sec, 2.132 real sec
sorting block 4, #reads = 1087364 mem = 536878820 peak = 842428416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281882, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 295, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (317.98, 154.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282239, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (318.55, 153.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682006 sequences (100000279 bp) in 1.206 CPU sec, 1.213 real sec...
[M::mem_process_seqs] Processed 682772 reads in 191.428 CPU sec, 6.089 real sec
[M::mem_process_seqs] Processed 682446 reads in 90.718 CPU sec, 2.906 real sec
sorting block 5, #reads = 1088036 mem = 536876803 peak = 842428416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282174, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 298, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (319.93, 154.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686600 sequences (100000182 bp) in 1.903 CPU sec, 1.988 real sec...
[M::process] read 686382 sequences (100000248 bp) in 1.180 CPU sec, 1.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281955, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 300, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (322.57, 156.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682060 reads in 123.853 CPU sec, 3.936 real sec
[M::mem_process_seqs] Processed 682006 reads in 78.397 CPU sec, 2.495 real sec
sorting block 6, #reads = 1086426 mem = 536875250 peak = 842428416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284178, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 279, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (304.14, 149.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685400 sequences (100000178 bp) in 1.616 CPU sec, 1.696 real sec...
[M::process] read 684940 sequences (100000023 bp) in 1.200 CPU sec, 1.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283643, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 282, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (306.53, 151.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686600 reads in 123.190 CPU sec, 3.922 real sec
[M::mem_process_seqs] Processed 686382 reads in 77.592 CPU sec, 2.466 real sec
sorting block 7, #reads = 1087199 mem = 536883083 peak = 842428416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283374, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 287, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (310.60, 152.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684950 sequences (100000185 bp) in 1.956 CPU sec, 2.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282982, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 290, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (311.83, 152.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684446 sequences (100000134 bp) in 1.260 CPU sec, 1.256 real sec...
[M::mem_process_seqs] Processed 685400 reads in 122.374 CPU sec, 3.889 real sec
sorting block 8, #reads = 1090448 mem = 536880091 peak = 842428416
[M::mem_process_seqs] Processed 684940 reads in 77.982 CPU sec, 2.474 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282876, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 290, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (314.55, 155.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1088842 mem = 536877362 peak = 870703104
[M::process] read 684316 sequences (100000128 bp) in 2.036 CPU sec, 2.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283006, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 293, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (318.09, 157.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682360 sequences (100000201 bp) in 1.250 CPU sec, 1.252 real sec...
[M::mem_process_seqs] Processed 684950 reads in 123.507 CPU sec, 3.927 real sec
[M::mem_process_seqs] Processed 684446 reads in 78.618 CPU sec, 2.498 real sec
sorting block 10, #reads = 1088936 mem = 536877836 peak = 870703104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282761, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 294, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (317.20, 155.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682628 sequences (100000101 bp) in 1.991 CPU sec, 2.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281721, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 303, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (323.76, 156.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682906 sequences (100000026 bp) in 1.280 CPU sec, 1.273 real sec...
[M::mem_process_seqs] Processed 684316 reads in 122.866 CPU sec, 3.902 real sec
[M::mem_process_seqs] Processed 682360 reads in 78.480 CPU sec, 2.486 real sec
sorting block 11, #reads = 1087823 mem = 536878857 peak = 870703104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282003, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 296, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (319.55, 155.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682434 sequences (100000221 bp) in 2.015 CPU sec, 2.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281656, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 296, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (319.01, 155.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682184 sequences (100000189 bp) in 1.290 CPU sec, 1.283 real sec...
[M::mem_process_seqs] Processed 682628 reads in 124.873 CPU sec, 3.969 real sec
[M::mem_process_seqs] Processed 682906 reads in 79.491 CPU sec, 2.524 real sec
sorting block 12, #reads = 1086959 mem = 536879688 peak = 870703104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281781, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (320.86, 156.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686798 sequences (100000194 bp) in 2.116 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281999, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 300, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (322.71, 157.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686178 sequences (100000188 bp) in 1.290 CPU sec, 1.294 real sec...
[M::mem_process_seqs] Processed 682434 reads in 125.432 CPU sec, 3.984 real sec
sorting block 13, #reads = 1086297 mem = 536879364 peak = 870703104
[M::mem_process_seqs] Processed 682184 reads in 80.525 CPU sec, 2.555 real sec
sorting block 14, #reads = 1085990 mem = 536877555 peak = 920559616
[M::process] read 685080 sequences (100000095 bp) in 2.076 CPU sec, 2.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283399, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 284, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (307.21, 150.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684592 sequences (100000136 bp) in 1.290 CPU sec, 1.294 real sec...
[M::mem_process_seqs] Processed 686178 reads in 71.826 CPU sec, 2.269 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283801, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 279, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (304.50, 149.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282745, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 289, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (311.57, 151.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686798 reads in 191.631 CPU sec, 6.088 real sec
[M::process] read 684472 sequences (100000252 bp) in 1.500 CPU sec, 1.509 real sec...
sorting block 15, #reads = 1087824 mem = 536878921 peak = 920559616
[M::mem_process_seqs] Processed 685080 reads in 91.587 CPU sec, 2.921 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282439, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 292, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (312.71, 150.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1088929 mem = 536879510 peak = 920559616
[M::process] read 683800 sequences (100000070 bp) in 2.413 CPU sec, 2.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283100, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 293, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (316.83, 156.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684592 reads in 125.520 CPU sec, 3.992 real sec
[M::mem_process_seqs] Processed 684472 reads in 80.346 CPU sec, 2.541 real sec
[M::process] read 682546 sequences (100000056 bp) in 1.533 CPU sec, 1.565 real sec...
sorting block 17, #reads = 1087995 mem = 536882500 peak = 920559616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282404, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 298, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (320.93, 157.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682322 sequences (100000108 bp) in 2.029 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282275, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 306, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (325.06, 156.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683800 reads in 125.594 CPU sec, 3.994 real sec
[M::process] read 682382 sequences (100000113 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 682546 reads in 80.414 CPU sec, 2.541 real sec
sorting block 18, #reads = 1086943 mem = 536877841 peak = 920559616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281852, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 309, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (327.68, 157.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682396 sequences (100000165 bp) in 2.241 CPU sec, 2.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281769, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 302, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (324.49, 157.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682322 reads in 126.456 CPU sec, 4.020 real sec
[M::process] read 682538 sequences (100000193 bp) in 1.419 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 682382 reads in 81.646 CPU sec, 2.580 real sec
sorting block 19, #reads = 1086784 mem = 536879332 peak = 920559616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282012, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 300, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (322.49, 157.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682044 sequences (100000056 bp) in 2.080 CPU sec, 2.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281784, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 298, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (321.28, 156.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685640 sequences (100000291 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_process_seqs] Processed 682396 reads in 125.246 CPU sec, 3.983 real sec
[M::mem_process_seqs] Processed 682538 reads in 80.290 CPU sec, 2.545 real sec
sorting block 20, #reads = 1085986 mem = 536877327 peak = 920559616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281585, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 301, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (324.44, 159.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1087030 mem = 536878713 peak = 938225664
[M::process] read 685980 sequences (100000079 bp) in 1.971 CPU sec, 2.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283075, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (304.64, 148.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685112 sequences (100000256 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_process_seqs] Processed 682044 reads in 126.078 CPU sec, 4.012 real sec
[M::mem_process_seqs] Processed 685640 reads in 80.463 CPU sec, 2.549 real sec
sorting block 22, #reads = 1085916 mem = 536880921 peak = 938225664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283269, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (304.73, 147.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684422 sequences (100000147 bp) in 2.180 CPU sec, 2.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283195, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 286, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.08, 148.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685980 reads in 121.976 CPU sec, 3.879 real sec
[M::process] read 684516 sequences (100000072 bp) in 1.415 CPU sec, 1.421 real sec...
[M::mem_process_seqs] Processed 685112 reads in 78.176 CPU sec, 2.470 real sec
sorting block 23, #reads = 1089569 mem = 536885860 peak = 938225664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282648, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 290, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (309.78, 147.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683996 sequences (100000020 bp) in 1.942 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282797, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 293, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (314.33, 152.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684422 reads in 121.966 CPU sec, 3.889 real sec
[M::process] read 683130 sequences (100000070 bp) in 1.443 CPU sec, 1.468 real sec...
[M::mem_process_seqs] Processed 684516 reads in 78.722 CPU sec, 2.484 real sec
sorting block 24, #reads = 1088203 mem = 536878976 peak = 938225664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282917, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 297, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (318.83, 155.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682734 sequences (100000071 bp) in 2.230 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282240, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 301, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (322.18, 156.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683996 reads in 123.443 CPU sec, 3.930 real sec
sorting block 25, #reads = 1087829 mem = 536878554 peak = 938225664
[M::mem_process_seqs] Processed 683130 reads in 79.729 CPU sec, 2.516 real sec
[M::process] read 682214 sequences (100000201 bp) in 1.518 CPU sec, 1.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281597, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 306, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (324.83, 156.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1087656 mem = 536877371 peak = 980963328
[M::process] read 682132 sequences (100000157 bp) in 2.072 CPU sec, 2.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281673, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 306, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (325.68, 157.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682734 reads in 127.020 CPU sec, 4.041 real sec
[M::process] read 681904 sequences (100000059 bp) in 1.340 CPU sec, 1.344 real sec...
[M::mem_process_seqs] Processed 682214 reads in 81.333 CPU sec, 2.573 real sec
sorting block 27, #reads = 1086522 mem = 536878956 peak = 980963328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281712, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 303, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (324.60, 157.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681980 sequences (100000212 bp) in 2.206 CPU sec, 2.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281724, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 302, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (324.36, 157.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682132 reads in 125.835 CPU sec, 3.998 real sec
[M::process] read 685594 sequences (100000262 bp) in 1.415 CPU sec, 1.418 real sec...
[M::mem_process_seqs] Processed 681904 reads in 80.662 CPU sec, 2.547 real sec
sorting block 28, #reads = 1086661 mem = 536878531 peak = 980963328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281672, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 299, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (322.35, 156.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687686 sequences (100000136 bp) in 2.132 CPU sec, 2.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283286, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (307.31, 151.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686982 sequences (100000087 bp) in 1.380 CPU sec, 1.382 real sec...
[M::mem_process_seqs] Processed 681980 reads in 129.146 CPU sec, 4.102 real sec
[M::mem_process_seqs] Processed 685594 reads in 83.609 CPU sec, 2.647 real sec
sorting block 29, #reads = 1086636 mem = 536881440 peak = 980963328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283940, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (297.23, 146.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686540 sequences (100000278 bp) in 2.354 CPU sec, 2.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284336, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 276, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (303.58, 150.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687686 reads in 128.906 CPU sec, 4.097 real sec
sorting block 30, #reads = 1085531 mem = 536883239 peak = 980963328
[M::process] read 685814 sequences (100000264 bp) in 1.520 CPU sec, 1.543 real sec...
[M::mem_process_seqs] Processed 686982 reads in 81.902 CPU sec, 2.585 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283794, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 278, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (305.52, 151.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1088688 mem = 536878940 peak = 980963328
[M::process] read 685236 sequences (100000286 bp) in 1.958 CPU sec, 2.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283661, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 283, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (308.27, 152.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686540 reads in 127.469 CPU sec, 4.067 real sec
[M::process] read 685184 sequences (100000073 bp) in 1.587 CPU sec, 1.608 real sec...
[M::mem_process_seqs] Processed 685814 reads in 82.683 CPU sec, 2.605 real sec
sorting block 32, #reads = 1087088 mem = 536882246 peak = 980963328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283523, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (309.73, 152.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683914 sequences (100000254 bp) in 2.202 CPU sec, 2.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283788, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (308.52, 151.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685236 reads in 129.183 CPU sec, 4.115 real sec
[M::process] read 682906 sequences (100000234 bp) in 1.395 CPU sec, 1.399 real sec...
[M::mem_process_seqs] Processed 685184 reads in 82.753 CPU sec, 2.617 real sec
sorting block 33, #reads = 1086210 mem = 536879425 peak = 980963328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282982, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (312.85, 152.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682624 sequences (100000025 bp) in 2.288 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282084, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (315.18, 150.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683914 reads in 130.596 CPU sec, 4.151 real sec
[M::process] read 682564 sequences (100000213 bp) in 1.435 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 682906 reads in 83.946 CPU sec, 2.652 real sec
sorting block 34, #reads = 1085728 mem = 536877911 peak = 980963328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282291, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (315.87, 151.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1083581 mem = 536879138 peak = 980963328
[M::process] read 683000 sequences (100000230 bp) in 2.125 CPU sec, 2.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282801, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (313.72, 148.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689672 sequences (100000218 bp) in 1.410 CPU sec, 1.407 real sec...
[M::mem_process_seqs] Processed 682624 reads in 131.737 CPU sec, 4.187 real sec
[M::mem_process_seqs] Processed 682564 reads in 84.313 CPU sec, 2.667 real sec
sorting block 36, #reads = 1084328 mem = 536877261 peak = 980963328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281661, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (313.01, 149.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688108 sequences (100000086 bp) in 2.261 CPU sec, 2.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284682, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 261, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (290.28, 144.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687204 sequences (100000196 bp) in 1.400 CPU sec, 1.404 real sec...
[M::mem_process_seqs] Processed 683000 reads in 133.546 CPU sec, 4.241 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284728, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (295.70, 145.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686520 sequences (100000132 bp) in 1.767 CPU sec, 1.767 real sec...
[M::mem_process_seqs] Processed 688108 reads in 71.776 CPU sec, 2.270 real sec
sorting block 37, #reads = 1082649 mem = 536879445 peak = 980963328
[M::mem_process_seqs] Processed 689672 reads in 177.463 CPU sec, 5.624 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284337, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (300.17, 147.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1087936 mem = 536881625 peak = 1058009088
[M::process] read 686208 sequences (100000257 bp) in 2.291 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283900, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 277, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (302.20, 147.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687204 reads in 141.716 CPU sec, 4.502 real sec
[M::process] read 685872 sequences (100000075 bp) in 1.626 CPU sec, 1.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283718, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (303.70, 147.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684608 sequences (100000162 bp) in 1.533 CPU sec, 1.539 real sec...
[M::mem_process_seqs] Processed 686208 reads in 75.594 CPU sec, 2.393 real sec
sorting block 39, #reads = 1087291 mem = 536878700 peak = 1058009088
[M::mem_process_seqs] Processed 686520 reads in 180.391 CPU sec, 5.720 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283655, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (303.52, 147.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1086237 mem = 536884970 peak = 1058009088
[M::process] read 683520 sequences (100000131 bp) in 2.288 CPU sec, 2.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283108, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (309.86, 150.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685872 reads in 140.427 CPU sec, 4.467 real sec
[M::mem_process_seqs] Processed 684608 reads in 82.420 CPU sec, 2.606 real sec
[M::process] read 683064 sequences (100000027 bp) in 2.128 CPU sec, 2.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282762, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (314.29, 151.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1086603 mem = 536879665 peak = 1058009088
[M::process] read 682550 sequences (100000011 bp) in 2.233 CPU sec, 2.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282281, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 295, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (316.78, 152.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683520 reads in 129.722 CPU sec, 4.149 real sec
[M::mem_process_seqs] Processed 683064 reads in 83.941 CPU sec, 2.657 real sec
sorting block 42, #reads = 1085319 mem = 536879180 peak = 1058009088
[M::process] read 682642 sequences (100000024 bp) in 2.113 CPU sec, 2.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282456, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 295, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (316.91, 152.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687744 sequences (100000070 bp) in 2.191 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281957, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (316.25, 152.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682550 reads in 133.084 CPU sec, 4.248 real sec
[M::mem_process_seqs] Processed 682642 reads in 86.971 CPU sec, 2.749 real sec
sorting block 43, #reads = 1083855 mem = 536877710 peak = 1058009088
[M::process] read 687680 sequences (100000267 bp) in 2.282 CPU sec, 2.444 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284147, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 270, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (296.91, 146.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686490 sequences (100000194 bp) in 2.209 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284047, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 272, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (297.38, 145.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687744 reads in 134.006 CPU sec, 4.283 real sec
sorting block 44, #reads = 1081928 mem = 536878966 peak = 1058009088
[M::mem_process_seqs] Processed 687680 reads in 83.127 CPU sec, 2.631 real sec
[M::process] read 686118 sequences (100000182 bp) in 2.309 CPU sec, 2.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283888, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 277, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (299.80, 144.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1087493 mem = 536875881 peak = 1058009088
[M::process] read 685628 sequences (100000035 bp) in 1.932 CPU sec, 2.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283900, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 279, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (302.27, 146.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686490 reads in 127.895 CPU sec, 4.091 real sec
[M::mem_process_seqs] Processed 686118 reads in 82.076 CPU sec, 2.594 real sec
[M::process] read 684794 sequences (100000182 bp) in 1.837 CPU sec, 1.932 real sec...
sorting block 46, #reads = 1088222 mem = 536879545 peak = 1058009088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283460, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (304.51, 146.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684236 sequences (100000002 bp) in 1.803 CPU sec, 1.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283451, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (306.86, 147.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685628 reads in 127.956 CPU sec, 4.072 real sec
[M::process] read 684254 sequences (100000200 bp) in 1.459 CPU sec, 1.479 real sec...
[M::mem_process_seqs] Processed 684794 reads in 82.249 CPU sec, 2.595 real sec
sorting block 47, #reads = 1087675 mem = 536878533 peak = 1058009088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283097, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (308.66, 147.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683730 sequences (100000142 bp) in 2.070 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282929, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (308.22, 146.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683636 sequences (100000005 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_process_seqs] Processed 684236 reads in 129.537 CPU sec, 4.120 real sec
[M::mem_process_seqs] Processed 684254 reads in 82.785 CPU sec, 2.622 real sec
sorting block 48, #reads = 1086326 mem = 536877971 peak = 1058009088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282704, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (311.25, 148.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683456 sequences (100000232 bp) in 2.087 CPU sec, 2.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283251, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (314.26, 150.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684180 sequences (100000173 bp) in 1.370 CPU sec, 1.362 real sec...
[M::mem_process_seqs] Processed 683730 reads in 129.314 CPU sec, 4.109 real sec
sorting block 49, #reads = 1085196 mem = 536878045 peak = 1058009088
[M::mem_process_seqs] Processed 683636 reads in 82.985 CPU sec, 2.629 real sec
sorting block 50, #reads = 1085043 mem = 536880668 peak = 1064951808
[M::process] read 689416 sequences (100000101 bp) in 2.092 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282571, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (311.67, 151.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687840 sequences (100000021 bp) in 1.390 CPU sec, 1.382 real sec...
[M::mem_process_seqs] Processed 684180 reads in 81.235 CPU sec, 2.564 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283383, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 295, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (315.54, 151.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285569, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 263, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (292.74, 145.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683456 reads in 206.703 CPU sec, 6.572 real sec
[M::process] read 686880 sequences (100000071 bp) in 1.455 CPU sec, 1.457 real sec...
sorting block 51, #reads = 1083293 mem = 536878591 peak = 1064951808
[M::mem_process_seqs] Processed 689416 reads in 97.554 CPU sec, 3.111 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284716, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 272, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (298.01, 145.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 52, #reads = 1086500 mem = 536879860 peak = 1064951808
[M::process] read 686374 sequences (100000066 bp) in 2.364 CPU sec, 2.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284026, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 278, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (301.78, 146.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687840 reads in 132.113 CPU sec, 4.198 real sec
[M::process] read 685758 sequences (100000216 bp) in 1.424 CPU sec, 1.428 real sec...
[M::mem_process_seqs] Processed 686880 reads in 83.516 CPU sec, 2.639 real sec
sorting block 53, #reads = 1086866 mem = 536881365 peak = 1064951808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284167, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (304.18, 147.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685080 sequences (100000056 bp) in 2.255 CPU sec, 2.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283477, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 283, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (306.73, 149.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684796 sequences (100000287 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_process_seqs] Processed 686374 reads in 131.432 CPU sec, 4.177 real sec
[M::mem_process_seqs] Processed 685758 reads in 83.241 CPU sec, 2.633 real sec
sorting block 54, #reads = 1086510 mem = 536878898 peak = 1064951808
[M::process] read 684600 sequences (100000249 bp) in 2.146 CPU sec, 2.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282953, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (310.16, 149.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683808 sequences (100000083 bp) in 1.377 CPU sec, 1.379 real sec...
[M::mem_process_seqs] Processed 684796 reads in 71.226 CPU sec, 2.243 real sec
sorting block 55, #reads = 1086087 mem = 536878898 peak = 1064951808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283341, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 287, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (309.64, 149.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282684, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (307.90, 147.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685080 reads in 201.876 CPU sec, 6.415 real sec
[M::process] read 683260 sequences (100000072 bp) in 1.461 CPU sec, 1.476 real sec...
[M::mem_process_seqs] Processed 684600 reads in 96.106 CPU sec, 3.066 real sec
sorting block 56, #reads = 1084545 mem = 536878387 peak = 1064951808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282775, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (310.69, 147.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682810 sequences (100000059 bp) in 2.422 CPU sec, 2.609 real sec...
[M::process] read 189588 sequences (27747045 bp) in 0.670 CPU sec, 0.672 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283562, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (312.73, 148.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683808 reads in 128.083 CPU sec, 4.087 real sec
sorting block 57, #reads = 1085663 mem = 536879657 peak = 1064951808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282234, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (313.63, 149.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682810 reads in 70.151 CPU sec, 2.399 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 78131, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (311.42, 147.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 189588 reads in 33.331 CPU sec, 1.097 real sec
[M::mem_process_seqs] Processed 683260 reads in 172.027 CPU sec, 5.704 real sec
sorting block 58, #reads = 1084075 mem = 536876655 peak = 1064951808
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 64535490
overall: 69881606144 mem 7949.071 user 323.314 sys 335.856 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.659_rd-N1-BDNA-1_B23KGCJLT4_1.L008\tPU:L008\tSM:659_rd-N1-BDNA-1_B23KGCJLT4_1\tLB:659_rd-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_rd-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 659_rd-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 335.901 sec; CPU: 8272.388 sec
sorting block 59, #reads = 725153 mem = 358498899 peak = 1129693184
uniq header count 1
merging 60 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3265036046
output reads: 64840783
bam_mem_sort: 60 calls 23.586 user 0.339 sys 24.029 real
bam_write: 60 calls 14.731 user 0.585 sys 15.424 real
execute: 1 calls 12.272 user 0.560 sys 352.775 real
merge_files: 1 calls 12.262 user 0.532 sys 16.367 real
parse_chunk: 955 calls 32.088 user 8.522 sys 41.257 real
read_chunk: 2575 calls 24.225 user 22.713 sys 218.224 real
sort_block: 1 calls 19.384 user 22.243 sys 211.127 real
write_chunk: 1618 calls 11.938 user 0.359 sys 15.710 real
overall: 2720501760 mem 655.946 user 38.215 sys 352.798 real
ls: cannot access '*.cram': No such file or directory