cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_rd-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
Received only 24 licenses out of 31 requested for sentieon:klib
Received only 24 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 2252.696 CPU sec, 524.079 real sec
[M::process] read 684842 sequences (100000244 bp) in 0.932 CPU sec, 0.939 real sec...
[M::process] read 683470 sequences (100000152 bp) in 1.210 CPU sec, 1.207 real sec...
[M::process] read 682982 sequences (100000154 bp) in 1.209 CPU sec, 1.210 real sec...
[M::process] read 682600 sequences (100000116 bp) in 1.140 CPU sec, 1.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282808, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 290, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (310.32, 148.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282834, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 300, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (316.79, 150.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282430, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 303, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (320.69, 152.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684842 reads in 224.437 CPU sec, 9.302 real sec
[M::mem_process_seqs] Processed 683470 reads in 144.176 CPU sec, 6.089 real sec
[M::mem_process_seqs] Processed 682982 reads in 155.936 CPU sec, 6.606 real sec
sorting block 0, #reads = 1087807 mem = 536878623 peak = 586129408
[M::process] read 682428 sequences (100000075 bp) in 1.863 CPU sec, 2.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282309, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 306, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (322.73, 152.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682354 sequences (100000080 bp) in 1.600 CPU sec, 1.713 real sec...
[M::process] read 682002 sequences (100000029 bp) in 1.147 CPU sec, 1.145 real sec...
Received only 24 licenses out of 31 requested for sentieon:klib
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282442, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 308, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (324.30, 153.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682600 reads in 138.729 CPU sec, 5.711 real sec
sorting block 1, #reads = 1087018 mem = 536880464 peak = 816009216
[M::mem_process_seqs] Processed 682428 reads in 85.033 CPU sec, 3.501 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282001, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 311, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (326.94, 155.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1086905 mem = 536879067 peak = 816009216
[M::process] read 681016 sequences (100000300 bp) in 2.436 CPU sec, 2.783 real sec...
[M::process] read 680366 sequences (100000090 bp) in 1.298 CPU sec, 1.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282231, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 312, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (328.54, 156.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682354 reads in 143.712 CPU sec, 5.954 real sec
[M::mem_process_seqs] Processed 682002 reads in 90.192 CPU sec, 3.728 real sec
sorting block 3, #reads = 1086631 mem = 536876785 peak = 816009216
[M::process] read 681626 sequences (100000028 bp) in 2.182 CPU sec, 2.381 real sec...
[M::process] read 681446 sequences (100000064 bp) in 1.270 CPU sec, 1.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281648, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 309, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (328.36, 157.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681626 reads in 79.135 CPU sec, 3.272 real sec
sorting block 4, #reads = 1085407 mem = 536879137 peak = 816009216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281459, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 318, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (333.35, 158.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281311, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 320, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (335.99, 159.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681828 sequences (100000104 bp) in 1.918 CPU sec, 1.925 real sec...
[M::mem_process_seqs] Processed 681016 reads in 227.817 CPU sec, 9.416 real sec
[M::mem_process_seqs] Processed 680366 reads in 171.990 CPU sec, 7.145 real sec
sorting block 5, #reads = 1086677 mem = 536878949 peak = 816009216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281548, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 306, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (326.30, 156.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684414 sequences (100000233 bp) in 2.426 CPU sec, 2.636 real sec...
[M::process] read 683222 sequences (100000011 bp) in 1.310 CPU sec, 1.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281700, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 302, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (323.97, 157.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681446 reads in 156.143 CPU sec, 6.603 real sec
[M::mem_process_seqs] Processed 681828 reads in 94.110 CPU sec, 3.892 real sec
sorting block 6, #reads = 1086815 mem = 536877317 peak = 816009216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283021, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 296, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (315.11, 151.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682692 sequences (100000127 bp) in 2.927 CPU sec, 3.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282115, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 305, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (323.76, 156.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682006 sequences (100000261 bp) in 1.402 CPU sec, 1.405 real sec...
[M::mem_process_seqs] Processed 684414 reads in 152.143 CPU sec, 6.312 real sec
[M::mem_process_seqs] Processed 683222 reads in 93.839 CPU sec, 3.878 real sec
sorting block 7, #reads = 1086623 mem = 536879293 peak = 816009216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282394, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 308, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (325.30, 155.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681818 sequences (100000280 bp) in 2.608 CPU sec, 3.004 real sec...
[M::process] read 681466 sequences (100000102 bp) in 1.310 CPU sec, 1.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281882, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 312, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (327.21, 155.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682692 reads in 156.417 CPU sec, 6.505 real sec
sorting block 8, #reads = 1087722 mem = 536880895 peak = 816009216
[M::mem_process_seqs] Processed 682006 reads in 99.753 CPU sec, 4.144 real sec
sorting block 9, #reads = 1085951 mem = 536878635 peak = 819056640
[M::process] read 681456 sequences (100000022 bp) in 2.766 CPU sec, 2.964 real sec...
[M::process] read 681204 sequences (100000100 bp) in 1.630 CPU sec, 1.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281338, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 316, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (332.09, 158.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681466 reads in 83.246 CPU sec, 3.406 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281352, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 313, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (328.76, 156.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680918 sequences (100000245 bp) in 1.897 CPU sec, 1.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281769, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 316, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (331.70, 157.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681818 reads in 230.994 CPU sec, 9.528 real sec
sorting block 10, #reads = 1085183 mem = 536879084 peak = 819056640
[M::mem_process_seqs] Processed 681456 reads in 103.743 CPU sec, 4.303 real sec
[M::process] read 681884 sequences (100000073 bp) in 3.350 CPU sec, 3.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281444, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 319, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (335.75, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681942 sequences (100000289 bp) in 1.417 CPU sec, 1.425 real sec...
[M::mem_process_seqs] Processed 680918 reads in 79.754 CPU sec, 3.247 real sec
sorting block 11, #reads = 1086309 mem = 536877569 peak = 819056640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281278, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 317, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (333.49, 158.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682104 sequences (100000104 bp) in 1.486 CPU sec, 1.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281449, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 306, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (326.95, 158.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681204 reads in 223.540 CPU sec, 9.179 real sec
[M::mem_process_seqs] Processed 681884 reads in 102.404 CPU sec, 4.244 real sec
sorting block 12, #reads = 1085909 mem = 536878499 peak = 896548864
Received only 24 licenses out of 31 requested for sentieon:klib
[M::process] read 684518 sequences (100000185 bp) in 2.334 CPU sec, 2.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281827, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 303, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (323.93, 156.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682602 sequences (100000173 bp) in 1.380 CPU sec, 1.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281803, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 301, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (323.30, 157.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282765, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 297, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (319.17, 156.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681942 reads in 206.282 CPU sec, 8.704 real sec
sorting block 13, #reads = 1085117 mem = 536879218 peak = 896548864
[M::mem_process_seqs] Processed 682104 reads in 150.703 CPU sec, 6.253 real sec
[M::mem_process_seqs] Processed 684518 reads in 163.054 CPU sec, 6.789 real sec
sorting block 14, #reads = 1084765 mem = 536879136 peak = 896548864
[M::process] read 681662 sequences (100000140 bp) in 2.453 CPU sec, 2.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281694, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 308, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (325.67, 156.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681386 sequences (100000045 bp) in 2.536 CPU sec, 2.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281923, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 314, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (329.00, 155.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682602 reads in 146.902 CPU sec, 6.057 real sec
[M::mem_process_seqs] Processed 681662 reads in 92.161 CPU sec, 3.753 real sec
sorting block 15, #reads = 1086557 mem = 536884834 peak = 896548864
[M::process] read 681826 sequences (100000144 bp) in 1.806 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281691, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 316, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (331.94, 157.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1085769 mem = 536880698 peak = 896548864
[M::process] read 681088 sequences (100000009 bp) in 1.996 CPU sec, 2.207 real sec...
[M::process] read 681054 sequences (100000065 bp) in 1.330 CPU sec, 1.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281596, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 315, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (331.02, 158.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681386 reads in 143.111 CPU sec, 5.916 real sec
[M::mem_process_seqs] Processed 681826 reads in 89.484 CPU sec, 3.682 real sec
sorting block 17, #reads = 1084947 mem = 536880457 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281953, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 317, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (332.71, 158.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680558 sequences (100000221 bp) in 2.116 CPU sec, 2.317 real sec...
[M::process] read 680902 sequences (100000215 bp) in 1.380 CPU sec, 1.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281253, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 318, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (334.00, 158.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681088 reads in 138.032 CPU sec, 5.705 real sec
[M::mem_process_seqs] Processed 681054 reads in 85.117 CPU sec, 3.487 real sec
sorting block 18, #reads = 1085840 mem = 536878905 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281303, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (336.22, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682108 sequences (100000042 bp) in 2.245 CPU sec, 2.371 real sec...
[M::process] read 682120 sequences (100000050 bp) in 1.400 CPU sec, 1.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281875, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 314, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (332.66, 160.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680558 reads in 143.850 CPU sec, 5.934 real sec
[M::mem_process_seqs] Processed 680902 reads in 89.395 CPU sec, 3.693 real sec
sorting block 19, #reads = 1085633 mem = 536877668 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281759, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 300, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (323.00, 157.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682802 sequences (100000123 bp) in 2.717 CPU sec, 2.936 real sec...
[M::process] read 683432 sequences (100000037 bp) in 1.410 CPU sec, 1.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281617, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 302, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (323.53, 157.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682108 reads in 141.293 CPU sec, 5.805 real sec
[M::mem_process_seqs] Processed 682120 reads in 86.868 CPU sec, 3.551 real sec
sorting block 20, #reads = 1085396 mem = 536879125 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282049, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 298, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (321.26, 157.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1086277 mem = 536882093 peak = 896548864
[M::process] read 681872 sequences (100000124 bp) in 2.074 CPU sec, 2.224 real sec...
[M::process] read 681486 sequences (100000215 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282289, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 300, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (321.37, 156.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682802 reads in 140.817 CPU sec, 5.825 real sec
[M::mem_process_seqs] Processed 683432 reads in 85.641 CPU sec, 3.509 real sec
sorting block 22, #reads = 1086493 mem = 536880086 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281805, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 309, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (326.60, 156.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680966 sequences (100000290 bp) in 2.317 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281853, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 312, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (329.33, 157.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681258 sequences (100000079 bp) in 1.780 CPU sec, 1.784 real sec...
[M::mem_process_seqs] Processed 681872 reads in 142.212 CPU sec, 6.010 real sec
[M::mem_process_seqs] Processed 681486 reads in 88.087 CPU sec, 3.596 real sec
sorting block 23, #reads = 1086798 mem = 536880308 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281870, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 316, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (333.25, 158.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680846 sequences (100000120 bp) in 2.108 CPU sec, 2.265 real sec...
[M::process] read 680648 sequences (100000020 bp) in 1.370 CPU sec, 1.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281598, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 313, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (331.09, 158.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680966 reads in 136.316 CPU sec, 5.624 real sec
[M::mem_process_seqs] Processed 681258 reads in 83.822 CPU sec, 3.435 real sec
sorting block 24, #reads = 1086580 mem = 536879166 peak = 896548864
Received only 24 licenses out of 31 requested for sentieon:klib
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281398, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 314, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (330.49, 156.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680702 sequences (100000157 bp) in 2.540 CPU sec, 2.682 real sec...
[M::process] read 681772 sequences (100000134 bp) in 1.400 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280713, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 314, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (332.16, 158.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680846 reads in 135.754 CPU sec, 5.557 real sec
sorting block 25, #reads = 1085877 mem = 536879853 peak = 896548864
[M::mem_process_seqs] Processed 680648 reads in 83.856 CPU sec, 3.430 real sec
sorting block 26, #reads = 1084998 mem = 536879951 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281572, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 313, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (331.93, 159.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682732 sequences (100000044 bp) in 2.267 CPU sec, 2.429 real sec...
[M::process] read 682360 sequences (100000031 bp) in 1.400 CPU sec, 1.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281162, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 302, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (323.37, 156.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680702 reads in 141.609 CPU sec, 6.022 real sec
[M::mem_process_seqs] Processed 681772 reads in 87.287 CPU sec, 3.592 real sec
sorting block 27, #reads = 1084620 mem = 536877714 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282842, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 295, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (319.09, 155.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684276 sequences (100000049 bp) in 2.312 CPU sec, 2.467 real sec...
[M::process] read 685064 sequences (100000148 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282078, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (319.15, 154.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682732 reads in 137.700 CPU sec, 5.651 real sec
[M::mem_process_seqs] Processed 682360 reads in 85.146 CPU sec, 3.713 real sec
sorting block 28, #reads = 1085731 mem = 536878764 peak = 896548864
[M::process] read 683418 sequences (100000287 bp) in 2.190 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282841, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (313.58, 154.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682952 sequences (100000124 bp) in 1.360 CPU sec, 1.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283123, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 289, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (314.82, 156.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282983, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 302, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (322.86, 157.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684276 reads in 198.382 CPU sec, 8.390 real sec
[M::mem_process_seqs] Processed 685064 reads in 141.494 CPU sec, 5.855 real sec
[M::mem_process_seqs] Processed 683418 reads in 152.893 CPU sec, 6.363 real sec
sorting block 29, #reads = 1085212 mem = 536876839 peak = 896548864
sorting block 30, #reads = 1087546 mem = 536884475 peak = 896548864
[M::process] read 682680 sequences (100000169 bp) in 2.339 CPU sec, 2.531 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282684, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 307, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (325.21, 157.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682854 sequences (100000107 bp) in 1.863 CPU sec, 1.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282422, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 306, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (324.66, 156.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682952 reads in 137.031 CPU sec, 5.713 real sec
[M::process] read 682312 sequences (100000156 bp) in 1.478 CPU sec, 1.512 real sec...
[M::mem_process_seqs] Processed 682680 reads in 84.672 CPU sec, 3.578 real sec
sorting block 31, #reads = 1086568 mem = 536878858 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282272, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 306, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (324.11, 155.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682128 sequences (100000295 bp) in 2.216 CPU sec, 2.402 real sec...
[M::process] read 681700 sequences (100000108 bp) in 1.400 CPU sec, 1.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282617, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 308, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (326.25, 156.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682854 reads in 140.764 CPU sec, 5.928 real sec
sorting block 32, #reads = 1086709 mem = 536879366 peak = 896548864
[M::mem_process_seqs] Processed 682312 reads in 84.219 CPU sec, 3.535 real sec
sorting block 33, #reads = 1085514 mem = 536880038 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282048, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 308, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (325.92, 156.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682534 sequences (100000048 bp) in 2.262 CPU sec, 2.477 real sec...
[M::process] read 682612 sequences (100000122 bp) in 1.410 CPU sec, 1.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282162, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 306, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (321.29, 150.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682128 reads in 139.368 CPU sec, 5.795 real sec
[M::mem_process_seqs] Processed 681700 reads in 83.567 CPU sec, 3.458 real sec
sorting block 34, #reads = 1085876 mem = 536881171 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281719, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 299, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (316.41, 148.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682042 sequences (100000208 bp) in 2.115 CPU sec, 2.247 real sec...
[M::process] read 685008 sequences (100000210 bp) in 1.430 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282581, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (315.36, 149.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682534 reads in 140.942 CPU sec, 5.863 real sec
[M::mem_process_seqs] Processed 682612 reads in 85.577 CPU sec, 3.493 real sec
sorting block 35, #reads = 1084853 mem = 536877045 peak = 896548864
[M::process] read 683822 sequences (100000299 bp) in 2.169 CPU sec, 2.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281664, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (313.91, 147.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683020 sequences (100000297 bp) in 1.390 CPU sec, 1.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283386, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 288, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (312.15, 153.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682042 reads in 142.699 CPU sec, 5.884 real sec
[M::mem_process_seqs] Processed 685008 reads in 85.548 CPU sec, 3.515 real sec
sorting block 36, #reads = 1084502 mem = 536877488 peak = 896548864
Received only 24 licenses out of 31 requested for sentieon:klib
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282415, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 298, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (318.01, 153.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682092 sequences (100000217 bp) in 2.146 CPU sec, 2.327 real sec...
[M::process] read 682214 sequences (100000189 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281992, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 304, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (322.81, 155.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683822 reads in 139.029 CPU sec, 5.718 real sec
sorting block 37, #reads = 1086492 mem = 536878837 peak = 896548864
[M::mem_process_seqs] Processed 683020 reads in 87.477 CPU sec, 3.587 real sec
sorting block 38, #reads = 1083875 mem = 536883538 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282270, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 310, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (327.04, 155.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682410 sequences (100000163 bp) in 2.059 CPU sec, 2.218 real sec...
[M::process] read 681836 sequences (100000072 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282244, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 309, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (325.27, 154.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682092 reads in 134.191 CPU sec, 5.509 real sec
[M::mem_process_seqs] Processed 682214 reads in 82.445 CPU sec, 3.389 real sec
sorting block 39, #reads = 1085259 mem = 536877158 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282271, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 308, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (324.71, 154.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681754 sequences (100000126 bp) in 2.358 CPU sec, 2.498 real sec...
[M::process] read 681568 sequences (100000257 bp) in 1.380 CPU sec, 1.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281971, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 309, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (324.46, 152.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682410 reads in 135.029 CPU sec, 5.538 real sec
[M::mem_process_seqs] Processed 681836 reads in 84.031 CPU sec, 3.448 real sec
sorting block 40, #reads = 1085034 mem = 536877177 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281993, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 309, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (325.60, 153.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682592 sequences (100000133 bp) in 2.341 CPU sec, 2.476 real sec...
[M::process] read 682868 sequences (100000032 bp) in 1.430 CPU sec, 1.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282069, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 309, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (325.29, 153.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681754 reads in 135.460 CPU sec, 5.554 real sec
[M::mem_process_seqs] Processed 681568 reads in 83.201 CPU sec, 3.416 real sec
sorting block 41, #reads = 1084422 mem = 536882037 peak = 896548864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282709, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 299, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (317.94, 151.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683302 sequences (100000066 bp) in 2.199 CPU sec, 2.333 real sec...
[M::process] read 684352 sequences (100000076 bp) in 1.390 CPU sec, 1.398 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282478, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 299, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (319.41, 153.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682592 reads in 140.446 CPU sec, 5.793 real sec
sorting block 42, #reads = 1085196 mem = 536878728 peak = 896548864
[M::mem_process_seqs] Processed 682868 reads in 84.482 CPU sec, 3.539 real sec
sorting block 43, #reads = 1085592 mem = 536880528 peak = 981688320
[M::process] read 682904 sequences (100000156 bp) in 2.209 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282531, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 294, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (315.06, 151.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681820 sequences (100000032 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283231, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 292, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (314.06, 152.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282118, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 303, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (320.98, 152.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683302 reads in 209.695 CPU sec, 8.719 real sec
[M::mem_process_seqs] Processed 684352 reads in 146.011 CPU sec, 6.049 real sec
[M::mem_process_seqs] Processed 682904 reads in 158.024 CPU sec, 6.581 real sec
sorting block 44, #reads = 1082372 mem = 536880105 peak = 981688320
[M::process] read 681452 sequences (100000233 bp) in 2.382 CPU sec, 2.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282120, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 310, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (324.90, 153.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1085886 mem = 536882051 peak = 981688320
[M::process] read 681620 sequences (100000252 bp) in 1.984 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281206, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 311, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (326.34, 153.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681820 reads in 137.894 CPU sec, 5.695 real sec
[M::mem_process_seqs] Processed 681452 reads in 85.477 CPU sec, 3.479 real sec
[M::process] read 681718 sequences (100000114 bp) in 1.766 CPU sec, 1.882 real sec...
sorting block 46, #reads = 1085039 mem = 536878850 peak = 981688320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281939, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 309, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (324.10, 151.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681170 sequences (100000116 bp) in 2.102 CPU sec, 2.327 real sec...
[M::process] read 680860 sequences (100000038 bp) in 1.330 CPU sec, 1.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282481, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 305, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (321.13, 149.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681620 reads in 137.929 CPU sec, 5.682 real sec
[M::mem_process_seqs] Processed 681718 reads in 84.291 CPU sec, 3.472 real sec
sorting block 47, #reads = 1085268 mem = 536879786 peak = 981688320
[M::process] read 681884 sequences (100000292 bp) in 2.106 CPU sec, 2.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282249, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 311, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (325.86, 152.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681170 reads in 76.973 CPU sec, 3.220 real sec
sorting block 48, #reads = 1084585 mem = 536878704 peak = 981688320
[M::process] read 682358 sequences (100000028 bp) in 1.991 CPU sec, 2.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282387, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 306, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (321.90, 151.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682660 sequences (100000093 bp) in 1.414 CPU sec, 1.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281705, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 312, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (326.74, 152.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681884 reads in 153.507 CPU sec, 6.332 real sec
Received only 24 licenses out of 31 requested for sentieon:klib
[M::mem_process_seqs] Processed 680860 reads in 163.304 CPU sec, 6.809 real sec
sorting block 49, #reads = 1083672 mem = 536878197 peak = 981688320
[M::process] read 684972 sequences (100000009 bp) in 1.981 CPU sec, 2.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282179, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 300, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (318.41, 150.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684408 sequences (100000163 bp) in 1.387 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282332, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 296, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (317.61, 152.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282602, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 285, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (311.10, 154.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682358 reads in 215.800 CPU sec, 8.977 real sec
[M::mem_process_seqs] Processed 682660 reads in 159.707 CPU sec, 6.627 real sec
sorting block 50, #reads = 1082655 mem = 536878078 peak = 981688320
[M::mem_process_seqs] Processed 684972 reads in 173.057 CPU sec, 7.225 real sec
sorting block 51, #reads = 1080175 mem = 536880027 peak = 981688320
[M::process] read 682792 sequences (100000192 bp) in 2.293 CPU sec, 2.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282821, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 297, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (319.47, 156.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681654 sequences (100000083 bp) in 1.765 CPU sec, 1.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282242, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 307, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (326.83, 158.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681784 sequences (100000287 bp) in 1.390 CPU sec, 1.382 real sec...
[M::mem_process_seqs] Processed 684408 reads in 144.895 CPU sec, 5.968 real sec
[M::mem_process_seqs] Processed 682792 reads in 88.062 CPU sec, 3.625 real sec
sorting block 52, #reads = 1084697 mem = 536883512 peak = 981688320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281980, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 316, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (332.43, 159.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681826 sequences (100000296 bp) in 2.189 CPU sec, 2.419 real sec...
[M::process] read 681410 sequences (100000121 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282474, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 314, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (330.67, 158.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681654 reads in 142.860 CPU sec, 5.941 real sec
[M::mem_process_seqs] Processed 681784 reads in 87.384 CPU sec, 3.591 real sec
sorting block 53, #reads = 1085259 mem = 536879832 peak = 981688320
[M::process] read 681346 sequences (100000108 bp) in 2.013 CPU sec, 2.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281926, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 310, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (327.23, 155.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681826 reads in 78.354 CPU sec, 3.290 real sec
sorting block 54, #reads = 1084495 mem = 536877470 peak = 989630464
[M::process] read 681596 sequences (100000048 bp) in 2.192 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281784, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 317, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (332.93, 159.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681980 sequences (100000135 bp) in 1.493 CPU sec, 1.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282062, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 313, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (329.62, 157.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681346 reads in 159.104 CPU sec, 6.583 real sec
[M::mem_process_seqs] Processed 681410 reads in 169.518 CPU sec, 7.096 real sec
sorting block 55, #reads = 1084808 mem = 536879853 peak = 989630464
[M::process] read 682552 sequences (100000025 bp) in 2.080 CPU sec, 2.269 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281768, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 307, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (326.68, 156.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 567090 sequences (83092199 bp) in 1.308 CPU sec, 1.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281564, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 297, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (316.88, 150.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681596 reads in 151.566 CPU sec, 6.315 real sec
sorting block 56, #reads = 1083694 mem = 536880820 peak = 989630464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282512, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (313.99, 149.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681980 reads in 160.703 CPU sec, 6.690 real sec
[M::mem_process_seqs] Processed 682552 reads in 174.876 CPU sec, 7.391 real sec
sorting block 57, #reads = 1084295 mem = 536877371 peak = 989630464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 234175, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (315.83, 152.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 567090 reads in 73.242 CPU sec, 3.251 real sec
sorting block 58, #reads = 1084297 mem = 536880178 peak = 989630464
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=24
reads: 64016052
overall: 69649207296 mem 8877.846 user 617.205 sys 840.120 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.659_rd-N1-BDNA-1_B23KGCJLT4_1.L003\tPU:L003\tSM:659_rd-N1-BDNA-1_B23KGCJLT4_1\tLB:659_rd-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_rd-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 659_rd-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 840.155 sec; CPU: 9495.054 sec
sorting block 59, #reads = 280279 mem = 139184324 peak = 989630464
uniq header count 1
merging 60 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 3120325200
output reads: 64320481
bam_mem_sort: 60 calls 32.281 user 0.763 sys 33.172 real
bam_write: 60 calls 23.793 user 1.184 sys 25.113 real
execute: 1 calls 16.148 user 1.367 sys 858.998 real
merge_files: 1 calls 16.128 user 1.336 sys 18.496 real
parse_chunk: 949 calls 36.761 user 10.341 sys 47.784 real
read_chunk: 2558 calls 28.108 user 23.172 sys 314.493 real
sort_block: 1 calls 22.678 user 22.522 sys 306.146 real
write_chunk: 1607 calls 15.677 user 1.116 sys 17.565 real
overall: 2435489792 mem 699.794 user 49.533 sys 859.018 real
ls: cannot access '*.cram': No such file or directory