cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_eZ4-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1339.192 CPU sec, 1365.458 real sec
[M::process] read 684644 sequences (100000110 bp) in 0.914 CPU sec, 0.920 real sec...
[M::process] read 683082 sequences (100000007 bp) in 1.119 CPU sec, 1.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283256, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 309, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (325.95, 155.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682630 sequences (100000274 bp) in 1.171 CPU sec, 1.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282190, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 318, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (334.62, 159.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682510 sequences (100000121 bp) in 1.178 CPU sec, 1.204 real sec...
[M::mem_process_seqs] Processed 684644 reads in 111.903 CPU sec, 3.583 real sec
[M::mem_process_seqs] Processed 683082 reads in 73.593 CPU sec, 2.380 real sec
sorting block 0, #reads = 1086233 mem = 536880202 peak = 601214976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281536, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (338.25, 160.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681966 sequences (100000105 bp) in 1.548 CPU sec, 1.743 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282247, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (339.63, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681506 sequences (100000150 bp) in 1.210 CPU sec, 1.209 real sec...
[M::mem_process_seqs] Processed 682630 reads in 110.859 CPU sec, 3.527 real sec
[M::mem_process_seqs] Processed 682510 reads in 73.179 CPU sec, 2.337 real sec
sorting block 1, #reads = 1084520 mem = 536879333 peak = 731762688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281793, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (342.38, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680774 sequences (100000010 bp) in 1.913 CPU sec, 2.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281569, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (345.26, 163.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681966 reads in 114.450 CPU sec, 3.642 real sec
[M::process] read 680918 sequences (100000177 bp) in 1.272 CPU sec, 1.275 real sec...
[M::mem_process_seqs] Processed 681506 reads in 76.480 CPU sec, 2.433 real sec
sorting block 2, #reads = 1084684 mem = 536880086 peak = 848637952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281426, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 334, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (350.64, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681622 sequences (100000046 bp) in 1.887 CPU sec, 1.959 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281477, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (349.36, 166.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681964 sequences (100000140 bp) in 1.300 CPU sec, 1.295 real sec...
[M::mem_process_seqs] Processed 680774 reads in 116.466 CPU sec, 3.704 real sec
sorting block 3, #reads = 1084983 mem = 536880084 peak = 904732672
[M::mem_process_seqs] Processed 680918 reads in 76.444 CPU sec, 2.431 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281198, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (343.19, 164.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1084633 mem = 536878209 peak = 904732672
[M::process] read 683804 sequences (100000207 bp) in 2.120 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281618, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 319, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (341.07, 166.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681622 reads in 119.752 CPU sec, 3.830 real sec
[M::mem_process_seqs] Processed 681964 reads in 78.945 CPU sec, 2.509 real sec
[M::process] read 683096 sequences (100000084 bp) in 1.697 CPU sec, 1.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282906, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 314, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (333.04, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1084630 mem = 536879700 peak = 904732672
[M::process] read 682208 sequences (100000134 bp) in 1.789 CPU sec, 1.841 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282460, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (340.67, 164.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683804 reads in 116.302 CPU sec, 3.717 real sec
[M::mem_process_seqs] Processed 683096 reads in 76.135 CPU sec, 2.412 real sec
[M::process] read 681830 sequences (100000052 bp) in 1.572 CPU sec, 1.636 real sec...
sorting block 6, #reads = 1085445 mem = 536881048 peak = 904732672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281916, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (343.21, 162.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681540 sequences (100000207 bp) in 1.700 CPU sec, 1.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281504, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (345.42, 164.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682208 reads in 117.204 CPU sec, 3.753 real sec
[M::process] read 681532 sequences (100000249 bp) in 1.415 CPU sec, 1.430 real sec...
[M::mem_process_seqs] Processed 681830 reads in 77.588 CPU sec, 2.460 real sec
sorting block 7, #reads = 1084725 mem = 536879448 peak = 925511680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282007, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (347.71, 165.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681104 sequences (100000113 bp) in 1.712 CPU sec, 1.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281891, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (347.68, 165.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681540 reads in 116.254 CPU sec, 3.721 real sec
[M::mem_process_seqs] Processed 681532 reads in 77.465 CPU sec, 2.452 real sec
[M::process] read 681280 sequences (100000214 bp) in 1.557 CPU sec, 1.591 real sec...
sorting block 8, #reads = 1083950 mem = 536877591 peak = 962465792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281254, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (349.26, 165.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1084809 mem = 536878644 peak = 970457088
[M::process] read 682180 sequences (100000180 bp) in 1.881 CPU sec, 1.946 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281941, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 331, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (349.08, 167.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681104 reads in 119.483 CPU sec, 3.824 real sec
[M::mem_process_seqs] Processed 681280 reads in 78.478 CPU sec, 2.484 real sec
[M::process] read 682332 sequences (100000244 bp) in 1.832 CPU sec, 1.865 real sec...
sorting block 10, #reads = 1083525 mem = 536879819 peak = 970457088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282482, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (342.07, 166.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684182 sequences (100000096 bp) in 1.930 CPU sec, 2.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281795, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 318, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (339.97, 166.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682180 reads in 120.520 CPU sec, 3.848 real sec
[M::mem_process_seqs] Processed 682332 reads in 79.348 CPU sec, 2.517 real sec
[M::process] read 682702 sequences (100000280 bp) in 1.583 CPU sec, 1.607 real sec...
sorting block 11, #reads = 1083461 mem = 536882510 peak = 970457088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282350, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 314, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (334.56, 163.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681690 sequences (100000139 bp) in 2.382 CPU sec, 2.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282357, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (341.02, 163.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684182 reads in 120.983 CPU sec, 3.875 real sec
[M::mem_process_seqs] Processed 682702 reads in 79.306 CPU sec, 2.518 real sec
sorting block 12, #reads = 1084811 mem = 536881933 peak = 970457088
[M::process] read 681418 sequences (100000186 bp) in 2.248 CPU sec, 2.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281931, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (345.43, 163.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281891, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (347.33, 164.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681230 sequences (100000024 bp) in 1.952 CPU sec, 2.029 real sec...
[M::mem_process_seqs] Processed 681690 reads in 117.819 CPU sec, 3.792 real sec
sorting block 13, #reads = 1084719 mem = 536877690 peak = 970457088
[M::mem_process_seqs] Processed 681418 reads in 77.674 CPU sec, 2.471 real sec
[M::process] read 680960 sequences (100000062 bp) in 2.050 CPU sec, 2.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281563, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (348.67, 165.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1083023 mem = 536876450 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281574, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (348.47, 164.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681230 reads in 116.057 CPU sec, 3.753 real sec
[M::mem_process_seqs] Processed 680960 reads in 76.412 CPU sec, 2.442 real sec
[M::process] read 680592 sequences (100000291 bp) in 2.269 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280963, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (351.94, 167.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680592 reads in 64.919 CPU sec, 2.168 real sec
sorting block 15, #reads = 1083398 mem = 536878858 peak = 998158336
[M::process] read 681046 sequences (100000064 bp) in 2.333 CPU sec, 2.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281293, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 329, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (348.68, 167.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681046 reads in 65.224 CPU sec, 2.164 real sec
[M::process] read 682332 sequences (100000013 bp) in 2.186 CPU sec, 2.418 real sec...
sorting block 16, #reads = 1082785 mem = 536884695 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282293, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 316, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (339.75, 166.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682332 reads in 65.197 CPU sec, 2.131 real sec
[M::process] read 682222 sequences (100000234 bp) in 2.650 CPU sec, 3.078 real sec...
sorting block 17, #reads = 1083053 mem = 536880998 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281835, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 318, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (339.89, 165.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682222 reads in 66.124 CPU sec, 2.153 real sec
[M::process] read 683678 sequences (100000231 bp) in 2.449 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282051, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 316, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (337.13, 165.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682038 sequences (100000188 bp) in 1.865 CPU sec, 1.962 real sec...
[M::mem_process_seqs] Processed 683678 reads in 65.944 CPU sec, 2.134 real sec
sorting block 18, #reads = 1084211 mem = 536878549 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281392, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (344.23, 164.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681192 sequences (100000194 bp) in 1.898 CPU sec, 1.991 real sec...
[M::mem_process_seqs] Processed 682038 reads in 64.707 CPU sec, 2.092 real sec
sorting block 19, #reads = 1085374 mem = 536882965 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281608, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (347.44, 165.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681192 reads in 65.742 CPU sec, 2.132 real sec
[M::process] read 680750 sequences (100000196 bp) in 2.225 CPU sec, 2.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281328, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (350.00, 166.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680826 sequences (100000100 bp) in 1.874 CPU sec, 1.964 real sec...
[M::mem_process_seqs] Processed 680750 reads in 65.410 CPU sec, 2.126 real sec
sorting block 20, #reads = 1083773 mem = 536880974 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281740, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (348.25, 165.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681064 sequences (100000238 bp) in 1.835 CPU sec, 1.930 real sec...
[M::mem_process_seqs] Processed 680826 reads in 65.283 CPU sec, 2.109 real sec
sorting block 21, #reads = 1083850 mem = 536879592 peak = 998158336
[M::process] read 680756 sequences (100000200 bp) in 1.798 CPU sec, 1.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281754, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (347.53, 165.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682018 sequences (100000091 bp) in 1.350 CPU sec, 1.351 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281246, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (347.83, 165.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681064 reads in 116.295 CPU sec, 3.661 real sec
[M::mem_process_seqs] Processed 680756 reads in 77.280 CPU sec, 2.435 real sec
[M::process] read 682892 sequences (100000067 bp) in 1.970 CPU sec, 2.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282413, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 320, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (341.07, 165.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 22, #reads = 1083449 mem = 536878573 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282078, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 311, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (334.90, 164.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682018 reads in 119.176 CPU sec, 3.829 real sec
[M::process] read 682540 sequences (100000103 bp) in 2.647 CPU sec, 2.871 real sec...
[M::mem_process_seqs] Processed 682892 reads in 79.591 CPU sec, 2.535 real sec
sorting block 23, #reads = 1083246 mem = 536883145 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281996, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (335.28, 163.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686126 sequences (100000165 bp) in 2.006 CPU sec, 2.113 real sec...
[M::mem_process_seqs] Processed 682540 reads in 68.392 CPU sec, 2.248 real sec
sorting block 24, #reads = 1084577 mem = 536878277 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283127, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 301, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (326.78, 163.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683996 sequences (100000152 bp) in 2.226 CPU sec, 2.485 real sec...
[M::mem_process_seqs] Processed 686126 reads in 70.002 CPU sec, 2.281 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282357, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 316, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (335.92, 164.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683314 sequences (100000078 bp) in 2.052 CPU sec, 2.165 real sec...
[M::mem_process_seqs] Processed 683996 reads in 67.128 CPU sec, 2.192 real sec
sorting block 25, #reads = 1083867 mem = 536886675 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282266, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 322, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (341.12, 165.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682858 sequences (100000063 bp) in 1.950 CPU sec, 2.075 real sec...
[M::mem_process_seqs] Processed 683314 reads in 67.117 CPU sec, 2.189 real sec
sorting block 26, #reads = 1084426 mem = 536880535 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282554, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (341.03, 164.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682858 reads in 65.866 CPU sec, 2.138 real sec
[M::process] read 682956 sequences (100000060 bp) in 2.370 CPU sec, 2.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282115, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (340.80, 164.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682690 sequences (100000032 bp) in 1.747 CPU sec, 1.891 real sec...
[M::mem_process_seqs] Processed 682956 reads in 68.198 CPU sec, 2.203 real sec
sorting block 27, #reads = 1085062 mem = 536882368 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282681, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (341.44, 164.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681946 sequences (100000246 bp) in 2.247 CPU sec, 2.422 real sec...
[M::mem_process_seqs] Processed 682690 reads in 67.676 CPU sec, 2.198 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282354, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (336.39, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 28, #reads = 1083700 mem = 536878529 peak = 998158336
[M::mem_process_seqs] Processed 681946 reads in 66.626 CPU sec, 2.162 real sec
[M::process] read 682388 sequences (100000052 bp) in 2.390 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282244, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (332.46, 156.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682388 reads in 67.559 CPU sec, 2.194 real sec
[M::process] read 683010 sequences (100000026 bp) in 2.532 CPU sec, 2.728 real sec...
sorting block 29, #reads = 1084182 mem = 536880873 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282459, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 311, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (328.66, 155.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683010 reads in 69.109 CPU sec, 2.237 real sec
[M::process] read 682610 sequences (100000080 bp) in 2.094 CPU sec, 2.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282465, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 311, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (329.73, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682610 reads in 70.230 CPU sec, 2.288 real sec
sorting block 30, #reads = 1082920 mem = 536886411 peak = 998158336
[M::process] read 685788 sequences (100000253 bp) in 2.827 CPU sec, 3.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283249, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 303, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (326.86, 162.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683198 sequences (100000026 bp) in 1.793 CPU sec, 1.928 real sec...
[M::mem_process_seqs] Processed 685788 reads in 68.820 CPU sec, 2.242 real sec
sorting block 31, #reads = 1083832 mem = 536877491 peak = 998158336
[M::process] read 682492 sequences (100000126 bp) in 1.772 CPU sec, 1.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282215, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (336.26, 161.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682264 sequences (100000243 bp) in 1.394 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282093, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (342.57, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683198 reads in 120.554 CPU sec, 3.801 real sec
[M::mem_process_seqs] Processed 682492 reads in 77.771 CPU sec, 2.454 real sec
[M::process] read 682340 sequences (100000234 bp) in 2.294 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282252, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (340.59, 162.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 32, #reads = 1083160 mem = 536879714 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281963, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (339.58, 161.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682264 reads in 116.648 CPU sec, 3.759 real sec
[M::mem_process_seqs] Processed 682340 reads in 78.030 CPU sec, 2.494 real sec
[M::process] read 682068 sequences (100000126 bp) in 2.834 CPU sec, 3.053 real sec...
sorting block 33, #reads = 1083453 mem = 536879990 peak = 998158336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281763, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (338.66, 160.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681656 sequences (100000206 bp) in 2.029 CPU sec, 2.172 real sec...
[M::mem_process_seqs] Processed 682068 reads in 66.927 CPU sec, 2.206 real sec
sorting block 34, #reads = 1083060 mem = 536879663 peak = 1069907968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282199, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 326, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (340.98, 160.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681656 reads in 66.180 CPU sec, 2.163 real sec
[M::process] read 682484 sequences (100000170 bp) in 2.549 CPU sec, 2.828 real sec...
sorting block 35, #reads = 1083143 mem = 536878631 peak = 1069907968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281975, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 318, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (336.56, 160.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683092 sequences (100000016 bp) in 1.728 CPU sec, 1.805 real sec...
[M::mem_process_seqs] Processed 682484 reads in 66.153 CPU sec, 2.136 real sec
[M::process] read 683614 sequences (100000094 bp) in 1.845 CPU sec, 1.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282548, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (334.98, 161.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684630 sequences (100000107 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282333, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 309, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (329.70, 159.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683092 reads in 124.047 CPU sec, 3.900 real sec
[M::process] read 682662 sequences (100000273 bp) in 1.755 CPU sec, 1.827 real sec...
sorting block 36, #reads = 1083583 mem = 536876161 peak = 1069907968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283097, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 310, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (330.50, 160.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684630 reads in 66.387 CPU sec, 2.108 real sec
[M::process] read 682130 sequences (100000263 bp) in 2.249 CPU sec, 2.385 real sec...
[M::mem_process_seqs] Processed 683614 reads in 201.134 CPU sec, 6.364 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282389, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 322, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (338.97, 161.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1084476 mem = 536880689 peak = 1069907968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282213, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 325, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (340.63, 160.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682662 reads in 132.513 CPU sec, 4.211 real sec
[M::process] read 681742 sequences (100000297 bp) in 2.281 CPU sec, 2.428 real sec...
[M::mem_process_seqs] Processed 682130 reads in 79.055 CPU sec, 2.524 real sec
sorting block 38, #reads = 1079809 mem = 536878908 peak = 1069907968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281720, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 325, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (340.00, 159.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682290 sequences (100000241 bp) in 2.048 CPU sec, 2.170 real sec...
[M::mem_process_seqs] Processed 681742 reads in 66.684 CPU sec, 2.200 real sec
sorting block 39, #reads = 1083001 mem = 536876954 peak = 1069907968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282548, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 320, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (335.67, 157.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682290 reads in 66.581 CPU sec, 2.178 real sec
[M::process] read 681508 sequences (100000028 bp) in 2.198 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281798, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (339.07, 158.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1082428 mem = 536880029 peak = 1069907968
[M::mem_process_seqs] Processed 681508 reads in 68.420 CPU sec, 2.241 real sec
[M::process] read 681312 sequences (100000271 bp) in 2.263 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281668, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 327, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (341.93, 159.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682602 sequences (100000223 bp) in 1.879 CPU sec, 1.967 real sec...
[M::mem_process_seqs] Processed 681312 reads in 68.934 CPU sec, 2.232 real sec
sorting block 41, #reads = 1080972 mem = 536879044 peak = 1069907968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282373, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (332.53, 157.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683106 sequences (100000148 bp) in 2.119 CPU sec, 2.267 real sec...
[M::mem_process_seqs] Processed 682602 reads in 67.952 CPU sec, 2.196 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282738, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (332.76, 160.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1081807 mem = 536880275 peak = 1069907968
[M::mem_process_seqs] Processed 683106 reads in 71.729 CPU sec, 2.325 real sec
[M::process] read 684668 sequences (100000285 bp) in 2.741 CPU sec, 2.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282417, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 304, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (328.44, 162.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684988 sequences (100000122 bp) in 2.130 CPU sec, 2.288 real sec...
[M::mem_process_seqs] Processed 684668 reads in 75.594 CPU sec, 2.439 real sec
sorting block 43, #reads = 1079742 mem = 536879977 peak = 1069907968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282665, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682644 sequences (100000208 bp) in 1.809 CPU sec, 1.903 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (200, 311, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (332.81, 164.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682070 sequences (100000287 bp) in 1.350 CPU sec, 1.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282797, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 328, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (345.74, 167.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684988 reads in 118.953 CPU sec, 3.781 real sec
[M::mem_process_seqs] Processed 682644 reads in 77.340 CPU sec, 2.439 real sec
[M::process] read 681898 sequences (100000155 bp) in 1.755 CPU sec, 1.822 real sec...
sorting block 44, #reads = 1082733 mem = 536878453 peak = 1069907968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282035, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (346.39, 165.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681598 sequences (100000265 bp) in 1.928 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281547, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (343.90, 164.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682070 reads in 118.609 CPU sec, 3.783 real sec
[M::mem_process_seqs] Processed 681898 reads in 78.586 CPU sec, 2.485 real sec
sorting block 45, #reads = 1082983 mem = 536882257 peak = 1069907968
[M::process] read 681274 sequences (100000030 bp) in 1.705 CPU sec, 1.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282096, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (344.74, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681744 sequences (100000027 bp) in 2.189 CPU sec, 2.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281745, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (348.42, 166.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681598 reads in 119.913 CPU sec, 3.819 real sec
[M::mem_process_seqs] Processed 681274 reads in 79.460 CPU sec, 2.519 real sec
sorting block 46, #reads = 1082224 mem = 536883962 peak = 1077264384
[M::process] read 682672 sequences (100000111 bp) in 2.180 CPU sec, 2.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281945, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (342.17, 164.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1081575 mem = 536877620 peak = 1077264384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281792, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 310, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (330.27, 158.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682850 sequences (100000128 bp) in 2.202 CPU sec, 2.384 real sec...
[M::mem_process_seqs] Processed 681744 reads in 121.688 CPU sec, 3.909 real sec
[M::mem_process_seqs] Processed 682672 reads in 81.118 CPU sec, 2.578 real sec
[M::process] read 193264 sequences (28304025 bp) in 0.661 CPU sec, 0.685 real sec...
sorting block 48, #reads = 1082042 mem = 536878530 peak = 1077264384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281637, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 308, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (329.84, 159.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 79738, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 307, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (330.26, 161.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682850 reads in 82.969 CPU sec, 2.710 real sec
[M::mem_process_seqs] Processed 193264 reads in 32.344 CPU sec, 1.055 real sec
sorting block 49, #reads = 1081734 mem = 536883181 peak = 1077264384
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 54101192
overall: 69560606720 mem 6305.621 user 303.474 sys 1549.069 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.659_eZ4-N1-BDNA-1_B23KGCJLT4_1.L003\tPU:L003\tSM:659_eZ4-N1-BDNA-1_B23KGCJLT4_1\tLB:659_eZ4-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_eZ4-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 659_eZ4-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 1549.118 sec; CPU: 6609.109 sec
sorting block 50, #reads = 180601 mem = 89877973 peak = 1077264384
uniq header count 1
merging 51 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2793061860
output reads: 54356382
bam_mem_sort: 51 calls 19.519 user 0.169 sys 19.772 real
bam_write: 51 calls 50.185 user 1.132 sys 51.469 real
execute: 1 calls 12.427 user 0.480 sys 1564.486 real
merge_files: 1 calls 12.409 user 0.439 sys 15.084 real
parse_chunk: 803 calls 27.670 user 8.232 sys 36.384 real
read_chunk: 2166 calls 18.489 user 15.088 sys 186.742 real
sort_block: 1 calls 13.332 user 14.763 sys 179.415 real
write_chunk: 1361 calls 12.058 user 0.288 sys 14.353 real
overall: 2567733248 mem 574.950 user 32.462 sys 1564.511 real
ls: cannot access '*.cram': No such file or directory