cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_rd-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1570.217 CPU sec, 773.055 real sec
[M::process] read 684550 sequences (100000068 bp) in 0.933 CPU sec, 0.936 real sec...
[M::process] read 684014 sequences (100000021 bp) in 1.229 CPU sec, 1.235 real sec...
[M::process] read 684150 sequences (100000115 bp) in 1.239 CPU sec, 1.237 real sec...
[M::process] read 683704 sequences (100000289 bp) in 1.180 CPU sec, 1.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283227, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 293, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (314.44, 152.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684014 reads in 68.312 CPU sec, 2.166 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283273, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 290, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (310.92, 149.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683430 sequences (100000015 bp) in 1.129 CPU sec, 1.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282891, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 292, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (313.78, 151.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684550 reads in 207.707 CPU sec, 6.581 real sec
sorting block 0, #reads = 1087779 mem = 536879842 peak = 600870912
[M::mem_process_seqs] Processed 684150 reads in 88.353 CPU sec, 2.829 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282175, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 296, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (316.68, 152.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683108 sequences (100000084 bp) in 1.779 CPU sec, 1.897 real sec...
[M::process] read 682840 sequences (100000167 bp) in 1.150 CPU sec, 1.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282446, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 297, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (317.16, 152.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683704 reads in 122.313 CPU sec, 3.920 real sec
sorting block 1, #reads = 1086026 mem = 536875993 peak = 745574400
[M::mem_process_seqs] Processed 683430 reads in 77.575 CPU sec, 2.493 real sec
sorting block 2, #reads = 1087168 mem = 536876823 peak = 872628224
[M::process] read 682850 sequences (100000190 bp) in 1.475 CPU sec, 1.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282140, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 297, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (318.72, 153.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682810 sequences (100000121 bp) in 1.224 CPU sec, 1.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282535, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 298, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (319.40, 153.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683108 reads in 120.871 CPU sec, 3.838 real sec
[M::mem_process_seqs] Processed 682840 reads in 76.541 CPU sec, 2.439 real sec
sorting block 3, #reads = 1085593 mem = 536880233 peak = 872628224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282564, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (319.27, 154.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682814 sequences (100000010 bp) in 1.722 CPU sec, 1.809 real sec...
[M::process] read 682496 sequences (100000198 bp) in 1.220 CPU sec, 1.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282188, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (316.61, 152.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682850 reads in 122.352 CPU sec, 3.884 real sec
[M::mem_process_seqs] Processed 682810 reads in 77.827 CPU sec, 2.474 real sec
sorting block 4, #reads = 1086494 mem = 536879189 peak = 872628224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282017, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (316.07, 153.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682382 sequences (100000106 bp) in 1.681 CPU sec, 1.805 real sec...
[M::process] read 684090 sequences (100000211 bp) in 1.220 CPU sec, 1.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281363, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (319.39, 155.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682814 reads in 122.832 CPU sec, 3.903 real sec
[M::mem_process_seqs] Processed 682496 reads in 77.869 CPU sec, 2.466 real sec
sorting block 5, #reads = 1085584 mem = 536878563 peak = 872628224
[M::process] read 683680 sequences (100000166 bp) in 1.854 CPU sec, 1.966 real sec...
[M::process] read 684228 sequences (100000163 bp) in 1.270 CPU sec, 1.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282674, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 296, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (314.87, 150.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684090 reads in 66.226 CPU sec, 2.084 real sec
sorting block 6, #reads = 1085662 mem = 536879853 peak = 872628224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281781, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (319.27, 154.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282347, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 297, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (317.27, 152.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683914 sequences (100000078 bp) in 1.304 CPU sec, 1.309 real sec...
[M::mem_process_seqs] Processed 682382 reads in 188.275 CPU sec, 5.975 real sec
[M::mem_process_seqs] Processed 683680 reads in 88.934 CPU sec, 2.848 real sec
sorting block 7, #reads = 1085993 mem = 536881471 peak = 933580800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282695, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 293, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (314.93, 152.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683706 sequences (100000267 bp) in 1.823 CPU sec, 1.943 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282089, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 297, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (318.35, 154.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683460 sequences (100000095 bp) in 1.300 CPU sec, 1.306 real sec...
[M::mem_process_seqs] Processed 684228 reads in 123.153 CPU sec, 3.911 real sec
sorting block 8, #reads = 1086782 mem = 536877550 peak = 933580800
[M::mem_process_seqs] Processed 683914 reads in 78.351 CPU sec, 2.480 real sec
sorting block 9, #reads = 1086894 mem = 536877814 peak = 933580800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282543, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 298, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (318.79, 154.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683106 sequences (100000252 bp) in 1.990 CPU sec, 2.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282704, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 299, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (319.03, 153.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683098 sequences (100000227 bp) in 1.340 CPU sec, 1.343 real sec...
[M::mem_process_seqs] Processed 683706 reads in 121.395 CPU sec, 3.846 real sec
[M::mem_process_seqs] Processed 683460 reads in 77.532 CPU sec, 2.445 real sec
sorting block 10, #reads = 1087031 mem = 536876581 peak = 933580800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281810, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 297, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (319.30, 154.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683082 sequences (100000036 bp) in 1.803 CPU sec, 1.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281936, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 296, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (318.84, 154.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683238 sequences (100000094 bp) in 1.370 CPU sec, 1.366 real sec...
[M::mem_process_seqs] Processed 683106 reads in 123.085 CPU sec, 3.909 real sec
[M::mem_process_seqs] Processed 683098 reads in 78.404 CPU sec, 2.477 real sec
sorting block 11, #reads = 1086491 mem = 536877839 peak = 933580800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282186, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 294, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (316.97, 154.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682864 sequences (100000003 bp) in 2.108 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282588, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 292, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (315.96, 154.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683406 sequences (100000018 bp) in 1.380 CPU sec, 1.378 real sec...
[M::mem_process_seqs] Processed 683082 reads in 125.183 CPU sec, 3.970 real sec
[M::mem_process_seqs] Processed 683238 reads in 79.783 CPU sec, 2.516 real sec
sorting block 12, #reads = 1087155 mem = 536879128 peak = 933580800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282096, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 295, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (318.95, 155.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684160 sequences (100000073 bp) in 1.851 CPU sec, 1.980 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282394, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 294, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (316.48, 153.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684436 sequences (100000179 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_process_seqs] Processed 682864 reads in 125.340 CPU sec, 3.983 real sec
sorting block 13, #reads = 1086033 mem = 536881369 peak = 933580800
[M::mem_process_seqs] Processed 683406 reads in 79.739 CPU sec, 2.518 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282552, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 298, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (315.39, 150.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1085211 mem = 536877625 peak = 933580800
[M::process] read 684294 sequences (100000025 bp) in 2.289 CPU sec, 2.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282582, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 293, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (313.34, 150.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684160 reads in 122.926 CPU sec, 3.905 real sec
[M::mem_process_seqs] Processed 684436 reads in 79.133 CPU sec, 2.494 real sec
[M::process] read 684176 sequences (100000065 bp) in 1.559 CPU sec, 1.588 real sec...
sorting block 15, #reads = 1086797 mem = 536883545 peak = 933580800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282710, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 294, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (313.98, 151.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683604 sequences (100000211 bp) in 1.827 CPU sec, 1.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282987, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 296, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (317.31, 154.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684294 reads in 120.294 CPU sec, 3.830 real sec
[M::process] read 683216 sequences (100000251 bp) in 1.518 CPU sec, 1.533 real sec...
sorting block 16, #reads = 1087531 mem = 536878562 peak = 933580800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282600, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 297, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (318.05, 153.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682980 sequences (100000118 bp) in 1.685 CPU sec, 1.685 real sec...
[M::mem_process_seqs] Processed 683604 reads in 67.223 CPU sec, 2.123 real sec
[M::mem_process_seqs] Processed 684176 reads in 170.131 CPU sec, 5.386 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282437, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 298, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (318.91, 153.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1087132 mem = 536877836 peak = 933580800
[M::process] read 682462 sequences (100000089 bp) in 1.964 CPU sec, 2.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282087, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 300, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (321.32, 155.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683216 reads in 132.809 CPU sec, 4.206 real sec
[M::mem_process_seqs] Processed 682980 reads in 78.483 CPU sec, 2.476 real sec
[M::process] read 682156 sequences (100000053 bp) in 1.835 CPU sec, 1.951 real sec...
sorting block 18, #reads = 1087067 mem = 536883738 peak = 933580800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281838, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 301, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (322.46, 156.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682240 sequences (100000056 bp) in 2.053 CPU sec, 2.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281542, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 301, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (322.23, 155.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682462 reads in 123.231 CPU sec, 3.924 real sec
[M::mem_process_seqs] Processed 682156 reads in 79.600 CPU sec, 2.512 real sec
[M::process] read 682164 sequences (100000088 bp) in 1.670 CPU sec, 1.744 real sec...
sorting block 19, #reads = 1086068 mem = 536881553 peak = 933580800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281791, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 299, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (321.52, 155.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684026 sequences (100000023 bp) in 1.822 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281676, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 299, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (321.43, 155.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682240 reads in 122.114 CPU sec, 3.888 real sec
[M::mem_process_seqs] Processed 682164 reads in 78.334 CPU sec, 2.467 real sec
sorting block 20, #reads = 1085107 mem = 536881998 peak = 933580800
[M::process] read 684388 sequences (100000098 bp) in 1.529 CPU sec, 1.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282071, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 291, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (313.49, 152.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1085808 mem = 536878453 peak = 937721856
[M::process] read 685320 sequences (100000233 bp) in 2.098 CPU sec, 2.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282066, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 292, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (312.77, 150.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684026 reads in 120.868 CPU sec, 3.844 real sec
[M::mem_process_seqs] Processed 684388 reads in 76.357 CPU sec, 2.411 real sec
[M::process] read 684846 sequences (100000215 bp) in 1.559 CPU sec, 1.595 real sec...
sorting block 22, #reads = 1086967 mem = 536879317 peak = 937721856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282449, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (307.45, 148.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684590 sequences (100000052 bp) in 1.828 CPU sec, 1.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282833, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (310.46, 150.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685320 reads in 121.126 CPU sec, 3.866 real sec
[M::mem_process_seqs] Processed 684846 reads in 77.803 CPU sec, 2.457 real sec
[M::process] read 683846 sequences (100000085 bp) in 1.526 CPU sec, 1.561 real sec...
sorting block 23, #reads = 1087551 mem = 536877827 peak = 937721856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282818, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (311.60, 151.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683350 sequences (100000052 bp) in 2.111 CPU sec, 2.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282200, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 293, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (314.97, 152.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684590 reads in 120.566 CPU sec, 3.831 real sec
[M::mem_process_seqs] Processed 683846 reads in 77.927 CPU sec, 2.453 real sec
[M::process] read 682952 sequences (100000146 bp) in 1.543 CPU sec, 1.580 real sec...
sorting block 24, #reads = 1087562 mem = 536882167 peak = 937721856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282696, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 294, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (316.34, 153.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682224 sequences (100000253 bp) in 1.913 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282191, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 297, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (318.78, 154.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683350 reads in 121.372 CPU sec, 3.870 real sec
sorting block 25, #reads = 1086995 mem = 536878785 peak = 937721856
[M::mem_process_seqs] Processed 682952 reads in 77.741 CPU sec, 2.449 real sec
[M::process] read 682048 sequences (100000002 bp) in 1.616 CPU sec, 1.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282067, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 300, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (320.52, 153.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1087896 mem = 536880393 peak = 937721856
[M::process] read 681724 sequences (100000037 bp) in 2.166 CPU sec, 2.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281627, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 302, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (323.17, 155.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682224 reads in 122.607 CPU sec, 3.898 real sec
[M::mem_process_seqs] Processed 682048 reads in 79.610 CPU sec, 2.507 real sec
[M::process] read 681928 sequences (100000096 bp) in 1.634 CPU sec, 1.676 real sec...
sorting block 27, #reads = 1085843 mem = 536882618 peak = 937721856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281738, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 303, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (323.75, 155.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686600 sequences (100000110 bp) in 1.971 CPU sec, 2.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282041, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 300, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (322.00, 155.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681724 reads in 122.740 CPU sec, 3.913 real sec
[M::mem_process_seqs] Processed 681928 reads in 79.512 CPU sec, 2.509 real sec
[M::process] read 686006 sequences (100000059 bp) in 1.697 CPU sec, 1.748 real sec...
sorting block 28, #reads = 1084643 mem = 536879921 peak = 937721856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283843, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 278, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (304.91, 150.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685138 sequences (100000166 bp) in 2.073 CPU sec, 2.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283833, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 284, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (309.03, 152.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686600 reads in 128.588 CPU sec, 4.082 real sec
[M::process] read 685208 sequences (100000160 bp) in 1.408 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 686006 reads in 81.001 CPU sec, 2.551 real sec
sorting block 29, #reads = 1085023 mem = 536885905 peak = 937721856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283858, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (307.79, 149.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684994 sequences (100000017 bp) in 1.953 CPU sec, 2.068 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283624, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 286, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.17, 148.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (99, 99, 99)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (99, 99)
[M::mem_pestat] mean and std.dev: (99.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (99, 99)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 685138 reads in 124.469 CPU sec, 3.955 real sec
[M::process] read 684682 sequences (100000185 bp) in 1.436 CPU sec, 1.441 real sec...
sorting block 30, #reads = 1087145 mem = 536879364 peak = 937721856
[M::mem_process_seqs] Processed 685208 reads in 79.187 CPU sec, 2.495 real sec
sorting block 31, #reads = 1086991 mem = 536879578 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283353, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (307.55, 148.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683988 sequences (100000222 bp) in 2.163 CPU sec, 2.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283396, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (308.94, 148.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683924 sequences (100000064 bp) in 1.390 CPU sec, 1.391 real sec...
[M::mem_process_seqs] Processed 684994 reads in 126.988 CPU sec, 4.027 real sec
[M::mem_process_seqs] Processed 684682 reads in 80.074 CPU sec, 2.527 real sec
sorting block 32, #reads = 1086364 mem = 536878030 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282801, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 290, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (310.99, 149.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683612 sequences (100000063 bp) in 1.934 CPU sec, 2.042 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282734, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (310.76, 148.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683988 reads in 126.413 CPU sec, 4.014 real sec
[M::process] read 683142 sequences (100000166 bp) in 1.450 CPU sec, 1.443 real sec...
[M::mem_process_seqs] Processed 683924 reads in 80.605 CPU sec, 2.543 real sec
sorting block 33, #reads = 1086320 mem = 536878877 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282694, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (309.22, 147.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683018 sequences (100000222 bp) in 2.199 CPU sec, 2.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282763, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (310.00, 147.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683798 sequences (100000095 bp) in 1.440 CPU sec, 1.439 real sec...
[M::mem_process_seqs] Processed 683612 reads in 129.436 CPU sec, 4.102 real sec
sorting block 34, #reads = 1084929 mem = 536878841 peak = 1036701696
[M::mem_process_seqs] Processed 683142 reads in 82.483 CPU sec, 2.606 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282290, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (311.43, 148.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1084649 mem = 536878908 peak = 1036701696
[M::process] read 685236 sequences (100000053 bp) in 1.973 CPU sec, 2.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282775, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (311.25, 149.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685088 sequences (100000220 bp) in 1.410 CPU sec, 1.416 real sec...
[M::mem_process_seqs] Processed 683018 reads in 131.466 CPU sec, 4.181 real sec
[M::mem_process_seqs] Processed 683798 reads in 83.806 CPU sec, 2.647 real sec
sorting block 36, #reads = 1083108 mem = 536884764 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282899, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 287, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (307.49, 147.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685296 sequences (100000105 bp) in 2.098 CPU sec, 2.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283542, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 285, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (306.12, 146.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685236 reads in 127.087 CPU sec, 4.042 real sec
[M::process] read 685214 sequences (100000109 bp) in 1.502 CPU sec, 1.524 real sec...
[M::mem_process_seqs] Processed 685088 reads in 81.114 CPU sec, 2.558 real sec
sorting block 37, #reads = 1085371 mem = 536880696 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283927, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 285, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.33, 146.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684736 sequences (100000212 bp) in 1.966 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283392, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (304.31, 144.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685296 reads in 127.619 CPU sec, 4.067 real sec
[M::process] read 684728 sequences (100000194 bp) in 1.542 CPU sec, 1.556 real sec...
[M::mem_process_seqs] Processed 685214 reads in 80.967 CPU sec, 2.550 real sec
sorting block 38, #reads = 1086691 mem = 536879775 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283267, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (307.29, 146.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684156 sequences (100000003 bp) in 2.013 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283545, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (307.67, 146.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684736 reads in 126.858 CPU sec, 4.039 real sec
[M::process] read 683908 sequences (100000105 bp) in 1.538 CPU sec, 1.557 real sec...
[M::mem_process_seqs] Processed 684728 reads in 80.154 CPU sec, 2.525 real sec
sorting block 39, #reads = 1086248 mem = 536881084 peak = 1036701696
sorting block 40, #reads = 1086840 mem = 536883950 peak = 1036701696
[M::process] read 683292 sequences (100000009 bp) in 1.959 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282958, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 292, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (312.24, 149.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683908 reads in 70.583 CPU sec, 2.217 real sec
[M::process] read 682972 sequences (100000168 bp) in 1.593 CPU sec, 1.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283046, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (309.76, 147.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282055, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (313.62, 149.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684156 reads in 195.773 CPU sec, 6.221 real sec
[M::mem_process_seqs] Processed 683292 reads in 92.875 CPU sec, 2.941 real sec
[M::process] read 683218 sequences (100000292 bp) in 1.909 CPU sec, 1.957 real sec...
sorting block 41, #reads = 1085801 mem = 536882304 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282516, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 294, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (314.44, 150.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1085709 mem = 536880791 peak = 1036701696
[M::process] read 684854 sequences (100000058 bp) in 2.386 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282267, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 294, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (315.67, 152.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682972 reads in 130.020 CPU sec, 4.133 real sec
[M::mem_process_seqs] Processed 683218 reads in 85.297 CPU sec, 2.685 real sec
[M::process] read 684976 sequences (100000153 bp) in 1.593 CPU sec, 1.639 real sec...
sorting block 43, #reads = 1084764 mem = 536876024 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283094, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (309.58, 151.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685452 sequences (100000105 bp) in 2.257 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283210, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 291, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (311.91, 151.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684854 reads in 132.892 CPU sec, 4.218 real sec
[M::process] read 685230 sequences (100000155 bp) in 1.614 CPU sec, 1.630 real sec...
[M::mem_process_seqs] Processed 684976 reads in 81.337 CPU sec, 2.565 real sec
sorting block 44, #reads = 1081410 mem = 536880623 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283931, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (305.92, 147.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684556 sequences (100000141 bp) in 2.477 CPU sec, 2.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283585, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (304.81, 145.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685452 reads in 128.053 CPU sec, 4.079 real sec
[M::mem_process_seqs] Processed 685230 reads in 81.494 CPU sec, 2.568 real sec
[M::process] read 684412 sequences (100000071 bp) in 1.909 CPU sec, 1.998 real sec...
sorting block 45, #reads = 1086699 mem = 536878281 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282796, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (307.96, 146.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683930 sequences (100000287 bp) in 2.111 CPU sec, 2.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283075, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (307.47, 146.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684556 reads in 128.495 CPU sec, 4.095 real sec
[M::mem_process_seqs] Processed 684412 reads in 82.458 CPU sec, 2.598 real sec
sorting block 46, #reads = 1086930 mem = 536880078 peak = 1036701696
[M::process] read 683316 sequences (100000155 bp) in 1.665 CPU sec, 1.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283227, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (309.64, 147.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1087161 mem = 536880146 peak = 1036701696
[M::process] read 683080 sequences (100000252 bp) in 2.064 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283069, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (313.86, 149.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682946 sequences (100000042 bp) in 1.350 CPU sec, 1.350 real sec...
[M::mem_process_seqs] Processed 683930 reads in 132.882 CPU sec, 4.220 real sec
[M::mem_process_seqs] Processed 683316 reads in 85.016 CPU sec, 2.690 real sec
sorting block 48, #reads = 1084036 mem = 536879714 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282638, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (313.48, 148.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683118 sequences (100000188 bp) in 2.093 CPU sec, 2.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282764, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 296, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (314.44, 148.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683080 reads in 126.505 CPU sec, 4.013 real sec
[M::process] read 682870 sequences (100000265 bp) in 1.420 CPU sec, 1.425 real sec...
[M::mem_process_seqs] Processed 682946 reads in 80.459 CPU sec, 2.537 real sec
sorting block 49, #reads = 1084403 mem = 536877472 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282607, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 297, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (315.93, 150.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686948 sequences (100000293 bp) in 2.257 CPU sec, 2.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282665, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 296, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (317.59, 152.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686302 sequences (100000301 bp) in 1.410 CPU sec, 1.404 real sec...
[M::mem_process_seqs] Processed 683118 reads in 133.326 CPU sec, 4.230 real sec
[M::mem_process_seqs] Processed 682870 reads in 86.330 CPU sec, 2.732 real sec
sorting block 50, #reads = 1084664 mem = 536880453 peak = 1036701696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283978, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 278, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (305.01, 151.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686024 sequences (100000233 bp) in 2.216 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283858, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 281, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (306.51, 151.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685300 sequences (100000048 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_process_seqs] Processed 686948 reads in 130.865 CPU sec, 4.158 real sec
sorting block 51, #reads = 1083012 mem = 536879414 peak = 1036701696
[M::mem_process_seqs] Processed 686302 reads in 82.511 CPU sec, 2.613 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284003, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (306.65, 150.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 52, #reads = 1087145 mem = 536880983 peak = 1103126528
[M::process] read 685076 sequences (100000158 bp) in 2.221 CPU sec, 2.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283020, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 285, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (308.62, 150.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686024 reads in 130.518 CPU sec, 4.144 real sec
[M::process] read 684574 sequences (100000038 bp) in 1.430 CPU sec, 1.427 real sec...
[M::mem_process_seqs] Processed 685300 reads in 82.961 CPU sec, 2.623 real sec
sorting block 53, #reads = 1086509 mem = 536880009 peak = 1103126528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283042, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 286, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (310.34, 151.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683462 sequences (100000019 bp) in 2.327 CPU sec, 2.483 real sec...
[M::process] read 682694 sequences (100000131 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283484, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (310.12, 150.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685076 reads in 145.546 CPU sec, 4.626 real sec
[M::mem_process_seqs] Processed 684574 reads in 96.344 CPU sec, 3.054 real sec
sorting block 54, #reads = 1084348 mem = 536878753 peak = 1103126528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283107, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (310.67, 146.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682224 sequences (100000222 bp) in 2.072 CPU sec, 2.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282657, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 295, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (313.02, 146.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681810 sequences (100000091 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 683462 reads in 129.286 CPU sec, 4.105 real sec
[M::mem_process_seqs] Processed 682694 reads in 80.443 CPU sec, 2.541 real sec
sorting block 55, #reads = 1081211 mem = 536879273 peak = 1103126528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282778, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 297, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (314.69, 147.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681738 sequences (100000241 bp) in 2.306 CPU sec, 2.439 real sec...
[M::process] read 253326 sequences (37146342 bp) in 0.720 CPU sec, 0.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282128, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (316.87, 148.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682224 reads in 125.801 CPU sec, 4.010 real sec
sorting block 56, #reads = 1085593 mem = 536879254 peak = 1103126528
[M::mem_process_seqs] Processed 681810 reads in 80.184 CPU sec, 2.554 real sec
sorting block 57, #reads = 1084204 mem = 536880695 peak = 1209864192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282083, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 301, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (319.28, 150.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 104878, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 300, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (319.81, 152.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681738 reads in 90.568 CPU sec, 2.954 real sec
[M::mem_process_seqs] Processed 253326 reads in 37.865 CPU sec, 1.228 real sec
sorting block 58, #reads = 1084357 mem = 536878909 peak = 1209864192
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 63848322
overall: 69589200896 mem 7779.063 user 240.884 sys 985.163 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.659_rd-N1-BDNA-1_B23KGCJLT4_1.L006\tPU:L006\tSM:659_rd-N1-BDNA-1_B23KGCJLT4_1\tLB:659_rd-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_rd-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 659_rd-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 985.213 sec; CPU: 8019.950 sec
sorting block 59, #reads = 84233 mem = 41885821 peak = 1209864192
uniq header count 1
merging 60 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3250715115
output reads: 64150731
bam_mem_sort: 60 calls 23.772 user 0.421 sys 24.336 real
bam_write: 60 calls 22.816 user 0.819 sys 23.825 real
execute: 1 calls 11.374 user 0.634 sys 1001.736 real
merge_files: 1 calls 11.350 user 0.616 sys 16.416 real
parse_chunk: 946 calls 31.302 user 8.271 sys 40.278 real
read_chunk: 2550 calls 23.206 user 21.393 sys 214.124 real
sort_block: 1 calls 18.169 user 20.878 sys 206.780 real
write_chunk: 1602 calls 11.046 user 0.460 sys 15.762 real
overall: 2707599360 mem 652.710 user 37.847 sys 1001.759 real
ls: cannot access '*.cram': No such file or directory