This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0002_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1530.586 CPU sec, 411.604 real sec
[M::process] read 688684 sequences (100000300 bp) in 0.909 CPU sec, 0.914 real sec...
[M::process] read 688510 sequences (100000232 bp) in 1.163 CPU sec, 1.175 real sec...
[M::process] read 687876 sequences (100000247 bp) in 1.153 CPU sec, 1.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285232, 0, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 182, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (214.62, 89.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 286, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (305.56, 146.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 355, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (270.75, 109.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687834 sequences (100000212 bp) in 1.100 CPU sec, 1.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285247, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 286, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (306.25, 147.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285519, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (308.43, 148.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 311, 3802)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11010)
[M::mem_pestat] mean and std.dev: (1538.60, 2085.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14614)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688684 reads in 193.054 CPU sec, 6.178 real sec
[M::mem_process_seqs] Processed 688510 reads in 129.020 CPU sec, 4.143 real sec
[M::mem_process_seqs] Processed 687876 reads in 140.212 CPU sec, 4.519 real sec
sorting block 0, #reads = 1073930 mem = 536879718 peak = 601616384
[M::process] read 687568 sequences (100000215 bp) in 1.627 CPU sec, 1.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285598, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (309.88, 149.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686814 sequences (100000189 bp) in 1.217 CPU sec, 1.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285322, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (311.09, 150.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687834 reads in 120.799 CPU sec, 3.838 real sec
sorting block 1, #reads = 1074726 mem = 536877971 peak = 837545984
[M::process] read 686756 sequences (100000167 bp) in 1.213 CPU sec, 1.228 real sec...
[M::mem_process_seqs] Processed 687568 reads in 75.993 CPU sec, 2.394 real sec
sorting block 2, #reads = 1074139 mem = 536878966 peak = 915156992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284963, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (313.35, 151.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686792 sequences (100000069 bp) in 1.446 CPU sec, 1.550 real sec...
[M::process] read 686698 sequences (100000294 bp) in 1.210 CPU sec, 1.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285470, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (312.75, 151.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686814 reads in 121.963 CPU sec, 3.890 real sec
[M::mem_process_seqs] Processed 686756 reads in 76.539 CPU sec, 2.435 real sec
sorting block 3, #reads = 1073645 mem = 536879178 peak = 915156992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284633, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 287, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (309.45, 149.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686298 sequences (100000143 bp) in 1.798 CPU sec, 1.886 real sec...
[M::process] read 687806 sequences (100000024 bp) in 1.190 CPU sec, 1.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285269, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 342, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (274.70, 103.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (311.00, 150.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686792 reads in 122.115 CPU sec, 3.886 real sec
[M::mem_process_seqs] Processed 686698 reads in 76.487 CPU sec, 2.431 real sec
sorting block 4, #reads = 1074424 mem = 536879863 peak = 915156992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285137, 0, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 234, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (87, 432)
[M::mem_pestat] mean and std.dev: (229.10, 70.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 512)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (313.47, 151.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 294, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (244.18, 125.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688566 sequences (100000297 bp) in 1.697 CPU sec, 1.834 real sec...
[M::process] read 688082 sequences (100000068 bp) in 1.250 CPU sec, 1.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284994, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 214, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (225.14, 112.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (309.91, 148.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686298 reads in 122.952 CPU sec, 3.921 real sec
sorting block 5, #reads = 1073046 mem = 536879870 peak = 915156992
[M::mem_process_seqs] Processed 687806 reads in 77.032 CPU sec, 2.447 real sec
sorting block 6, #reads = 1073225 mem = 536882103 peak = 915156992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284900, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 155, 155)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269)
[M::mem_pestat] mean and std.dev: (139.50, 39.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 326)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (308.66, 149.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 230, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (155, 280)
[M::mem_pestat] mean and std.dev: (216.22, 15.91)
[M::mem_pestat] low and high boundaries for proper pairs: (130, 305)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687882 sequences (100000078 bp) in 1.948 CPU sec, 2.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285841, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (310.62, 150.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687504 sequences (100000066 bp) in 1.300 CPU sec, 1.291 real sec...
[M::mem_process_seqs] Processed 688566 reads in 124.747 CPU sec, 3.975 real sec
[M::mem_process_seqs] Processed 688082 reads in 78.305 CPU sec, 2.486 real sec
sorting block 7, #reads = 1074102 mem = 536884779 peak = 915156992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285812, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (311.25, 151.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687282 sequences (100000144 bp) in 1.803 CPU sec, 1.965 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284990, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 158, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (158.67, 60.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 729)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (312.98, 151.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 267, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (233.90, 91.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 691)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687248 sequences (100000032 bp) in 1.310 CPU sec, 1.311 real sec...
[M::mem_process_seqs] Processed 687882 reads in 123.917 CPU sec, 3.961 real sec
[M::mem_process_seqs] Processed 687504 reads in 79.063 CPU sec, 2.517 real sec
sorting block 8, #reads = 1074135 mem = 536877288 peak = 915156992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285524, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (312.71, 151.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687276 sequences (100000127 bp) in 2.169 CPU sec, 2.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285250, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (311.79, 151.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686756 sequences (100000021 bp) in 1.370 CPU sec, 1.367 real sec...
[M::mem_process_seqs] Processed 687282 reads in 126.123 CPU sec, 4.011 real sec
[M::mem_process_seqs] Processed 687248 reads in 79.596 CPU sec, 2.521 real sec
sorting block 9, #reads = 1073629 mem = 536879117 peak = 915156992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285207, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 286, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (309.69, 150.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687128 sequences (100000002 bp) in 1.917 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285357, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (311.10, 151.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688086 sequences (100000161 bp) in 1.340 CPU sec, 1.340 real sec...
[M::mem_process_seqs] Processed 687276 reads in 126.557 CPU sec, 4.035 real sec
sorting block 10, #reads = 1073365 mem = 536881574 peak = 915156992
[M::mem_process_seqs] Processed 686756 reads in 80.023 CPU sec, 2.545 real sec
sorting block 11, #reads = 1073566 mem = 536878985 peak = 938102784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285131, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 240, 952)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2540)
[M::mem_pestat] mean and std.dev: (416.45, 346.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3334)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 289, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (310.27, 150.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688664 sequences (100000029 bp) in 2.169 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285509, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (309.76, 148.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687128 reads in 125.423 CPU sec, 3.996 real sec
[M::process] read 688454 sequences (100000218 bp) in 1.370 CPU sec, 1.366 real sec...
[M::mem_process_seqs] Processed 688086 reads in 78.749 CPU sec, 2.495 real sec
sorting block 12, #reads = 1073186 mem = 536881126 peak = 938102784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286010, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 162, 162)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 258)
[M::mem_pestat] mean and std.dev: (151.27, 39.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 309)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (306.95, 148.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687876 sequences (100000021 bp) in 1.923 CPU sec, 2.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285786, 0, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 170, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (186.10, 44.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 496)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (309.68, 150.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 194, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (221.31, 125.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687560 sequences (100000104 bp) in 1.360 CPU sec, 1.361 real sec...
[M::mem_process_seqs] Processed 688664 reads in 125.662 CPU sec, 4.011 real sec
[M::mem_process_seqs] Processed 688454 reads in 79.362 CPU sec, 2.517 real sec
sorting block 13, #reads = 1074638 mem = 536877286 peak = 938102784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285655, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (312.05, 150.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687004 sequences (100000081 bp) in 2.202 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285555, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (312.39, 151.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687876 reads in 126.422 CPU sec, 4.031 real sec
[M::process] read 686506 sequences (100000040 bp) in 1.434 CPU sec, 1.433 real sec...
sorting block 14, #reads = 1074762 mem = 536879507 peak = 938102784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285043, 0, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (315.31, 152.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 247, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (60, 405)
[M::mem_pestat] mean and std.dev: (231.94, 61.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation RR
[M::process] read 685960 sequences (100000209 bp) in 1.465 CPU sec, 1.465 real sec...
[M::mem_process_seqs] Processed 687004 reads in 74.276 CPU sec, 2.545 real sec
sorting block 15, #reads = 1073903 mem = 536878064 peak = 938102784
[M::mem_process_seqs] Processed 687560 reads in 172.047 CPU sec, 5.652 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285048, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (315.48, 152.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686250 sequences (100000297 bp) in 2.108 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285001, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 726, 851)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2269)
[M::mem_pestat] mean and std.dev: (506.50, 327.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2978)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 295, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (316.22, 152.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686506 reads in 132.930 CPU sec, 4.232 real sec
[M::mem_process_seqs] Processed 685960 reads in 77.893 CPU sec, 2.457 real sec
sorting block 16, #reads = 1073315 mem = 536879106 peak = 938102784
[M::process] read 688168 sequences (100000078 bp) in 1.703 CPU sec, 1.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284521, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 292, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (314.39, 152.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 170, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204)
[M::mem_pestat] mean and std.dev: (247.62, 148.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1561)
[M::mem_pestat] skip orientation RR
sorting block 17, #reads = 1072662 mem = 536880312 peak = 938102784
[M::process] read 688180 sequences (100000101 bp) in 2.064 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285061, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 287, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (308.57, 150.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686250 reads in 122.171 CPU sec, 3.895 real sec
[M::mem_process_seqs] Processed 688168 reads in 78.346 CPU sec, 2.474 real sec
[M::process] read 689484 sequences (100000156 bp) in 1.601 CPU sec, 1.661 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285235, 1, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 287, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (307.30, 147.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 202, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411)
[M::mem_pestat] mean and std.dev: (190.86, 62.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 499)
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1072404 mem = 536878693 peak = 938102784
[M::process] read 688868 sequences (100000228 bp) in 2.095 CPU sec, 2.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286474, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (301.83, 146.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 234, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (226.40, 92.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 723)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688180 reads in 119.208 CPU sec, 3.792 real sec
[M::mem_process_seqs] Processed 689484 reads in 76.650 CPU sec, 2.417 real sec
[M::process] read 688314 sequences (100000139 bp) in 1.643 CPU sec, 1.708 real sec...
sorting block 19, #reads = 1074765 mem = 536877932 peak = 938102784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285814, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 173, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (144.56, 54.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (305.76, 148.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687644 sequences (100000115 bp) in 2.053 CPU sec, 2.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285495, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 286, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (306.96, 148.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688868 reads in 118.768 CPU sec, 3.785 real sec
[M::mem_process_seqs] Processed 688314 reads in 76.188 CPU sec, 2.406 real sec
[M::process] read 687046 sequences (100000022 bp) in 1.724 CPU sec, 1.819 real sec...
sorting block 20, #reads = 1075996 mem = 536879461 peak = 938102784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285627, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (309.65, 150.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685858 sequences (100000059 bp) in 2.166 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285212, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (311.64, 150.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 175, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509)
[M::mem_pestat] mean and std.dev: (201.36, 97.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687644 reads in 120.795 CPU sec, 3.854 real sec
[M::mem_process_seqs] Processed 687046 reads in 77.505 CPU sec, 2.452 real sec
sorting block 21, #reads = 1074968 mem = 536879327 peak = 938102784
[M::process] read 685780 sequences (100000157 bp) in 1.908 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284801, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 295, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (315.48, 151.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 22, #reads = 1074366 mem = 536877843 peak = 938102784
[M::process] read 685550 sequences (100000011 bp) in 1.791 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284859, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (317.27, 153.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685858 reads in 122.404 CPU sec, 3.916 real sec
[M::mem_process_seqs] Processed 685780 reads in 78.437 CPU sec, 2.478 real sec
[M::process] read 689908 sequences (100000001 bp) in 1.896 CPU sec, 2.004 real sec...
sorting block 23, #reads = 1072047 mem = 536879646 peak = 938102784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284410, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (315.45, 151.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 184, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (180.89, 57.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] skip orientation RR
[M::process] read 690218 sequences (100000002 bp) in 2.056 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286272, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 280, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (303.33, 149.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 260, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (297.38, 195.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685550 reads in 125.120 CPU sec, 3.985 real sec
[M::mem_process_seqs] Processed 689908 reads in 81.341 CPU sec, 2.566 real sec
[M::process] read 689770 sequences (100000046 bp) in 1.767 CPU sec, 1.870 real sec...
sorting block 24, #reads = 1072739 mem = 536879374 peak = 938102784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286866, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 248, 896)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2348)
[M::mem_pestat] mean and std.dev: (350.50, 287.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3074)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 279, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (302.55, 148.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 689488 sequences (100000240 bp) in 1.772 CPU sec, 1.893 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286381, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (301.81, 147.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690218 reads in 126.617 CPU sec, 4.036 real sec
[M::mem_process_seqs] Processed 689770 reads in 80.232 CPU sec, 2.529 real sec
[M::process] read 689036 sequences (100000200 bp) in 1.438 CPU sec, 1.475 real sec...
sorting block 25, #reads = 1073724 mem = 536879000 peak = 938102784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286464, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (302.40, 146.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1074564 mem = 536880239 peak = 942436352
[M::process] read 688426 sequences (100000053 bp) in 2.177 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286368, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 282, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (301.89, 145.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (108, 271, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (212.82, 86.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 868)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689488 reads in 126.933 CPU sec, 4.032 real sec
[M::process] read 687950 sequences (100000272 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_process_seqs] Processed 689036 reads in 81.579 CPU sec, 2.578 real sec
sorting block 27, #reads = 1073927 mem = 536878206 peak = 942436352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 285600, 0, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 202, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (192.93, 107.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (303.89, 146.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687702 sequences (100000047 bp) in 1.856 CPU sec, 1.963 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285726, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (304.73, 146.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687338 sequences (100000027 bp) in 1.356 CPU sec, 1.364 real sec...
[M::mem_process_seqs] Processed 688426 reads in 126.369 CPU sec, 4.016 real sec
[M::mem_process_seqs] Processed 687950 reads in 80.037 CPU sec, 2.532 real sec
sorting block 28, #reads = 1073976 mem = 536881234 peak = 942436352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285299, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (303.21, 144.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 155, 2493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7233)
[M::mem_pestat] mean and std.dev: (924.50, 1391.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9603)
[M::mem_pestat] skip orientation RR
[M::process] read 686890 sequences (100000227 bp) in 2.239 CPU sec, 2.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285289, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (303.88, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687702 reads in 128.949 CPU sec, 4.093 real sec
[M::process] read 689772 sequences (100000080 bp) in 1.400 CPU sec, 1.395 real sec...
sorting block 29, #reads = 1073262 mem = 536879714 peak = 942436352
[M::process] read 689420 sequences (100000209 bp) in 1.430 CPU sec, 1.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284911, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (307.14, 146.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (100, 127, 269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (150.50, 76.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686890 reads in 76.479 CPU sec, 2.607 real sec
[M::mem_process_seqs] Processed 687338 reads in 176.760 CPU sec, 5.790 real sec
sorting block 30, #reads = 1070375 mem = 536880223 peak = 942436352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286487, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (301.71, 146.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689394 sequences (100000080 bp) in 2.059 CPU sec, 2.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286858, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (301.18, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689772 reads in 139.025 CPU sec, 4.415 real sec
[M::mem_process_seqs] Processed 689420 reads in 80.348 CPU sec, 2.535 real sec
[M::process] read 689548 sequences (100000295 bp) in 1.516 CPU sec, 1.559 real sec...
sorting block 31, #reads = 1072263 mem = 536879906 peak = 979378176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286517, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (298.45, 142.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688706 sequences (100000117 bp) in 1.993 CPU sec, 2.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286676, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (299.08, 142.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 209, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (54, 384)
[M::mem_pestat] mean and std.dev: (183.89, 48.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689394 reads in 127.474 CPU sec, 4.067 real sec
[M::process] read 688340 sequences (100000228 bp) in 1.400 CPU sec, 1.425 real sec...
[M::mem_process_seqs] Processed 689548 reads in 81.415 CPU sec, 2.568 real sec
sorting block 32, #reads = 1073866 mem = 536878305 peak = 979378176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286258, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 201, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482)
[M::mem_pestat] mean and std.dev: (182.30, 65.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 595)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (301.36, 143.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 33, #reads = 1073893 mem = 536886077 peak = 1028501504
[M::process] read 687818 sequences (100000276 bp) in 2.239 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285754, 1, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (294, 340, 953)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2271)
[M::mem_pestat] mean and std.dev: (390.45, 248.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2930)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.50, 145.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 174, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477)
[M::mem_pestat] mean and std.dev: (198.00, 83.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688706 reads in 127.217 CPU sec, 4.047 real sec
[M::process] read 687472 sequences (100000243 bp) in 1.348 CPU sec, 1.354 real sec...
[M::mem_process_seqs] Processed 688340 reads in 80.366 CPU sec, 2.547 real sec
sorting block 34, #reads = 1074637 mem = 536884453 peak = 1028501504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285630, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (305.32, 145.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686994 sequences (100000025 bp) in 2.072 CPU sec, 2.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285547, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (307.83, 147.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (100, 131, 223)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (151.50, 60.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 592)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687818 reads in 127.576 CPU sec, 4.059 real sec
[M::process] read 687248 sequences (100000234 bp) in 1.413 CPU sec, 1.420 real sec...
[M::mem_process_seqs] Processed 687472 reads in 81.128 CPU sec, 2.561 real sec
sorting block 35, #reads = 1073260 mem = 536878891 peak = 1028501504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285378, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 288, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (308.52, 147.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690126 sequences (100000155 bp) in 2.229 CPU sec, 2.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285265, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.36, 148.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689664 sequences (100000228 bp) in 1.320 CPU sec, 1.325 real sec...
[M::mem_process_seqs] Processed 686994 reads in 134.764 CPU sec, 4.281 real sec
sorting block 36, #reads = 1073253 mem = 536878960 peak = 1028501504
[M::mem_process_seqs] Processed 687248 reads in 89.068 CPU sec, 2.819 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286269, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 219, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511)
[M::mem_pestat] mean and std.dev: (201.00, 85.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 281, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (302.80, 148.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 689268 sequences (100000151 bp) in 1.997 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286483, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (300.99, 145.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688628 sequences (100000031 bp) in 1.320 CPU sec, 1.313 real sec...
[M::mem_process_seqs] Processed 690126 reads in 129.283 CPU sec, 4.111 real sec
sorting block 37, #reads = 1069218 mem = 536878072 peak = 1028501504
[M::mem_process_seqs] Processed 689664 reads in 82.153 CPU sec, 2.604 real sec
sorting block 38, #reads = 1073771 mem = 536880546 peak = 1048707072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286685, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 281, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (299.85, 143.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 275, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (303.78, 160.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1380)
[M::mem_pestat] skip orientation RR
[M::process] read 688414 sequences (100000134 bp) in 2.067 CPU sec, 2.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286109, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (301.65, 143.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689268 reads in 125.526 CPU sec, 3.991 real sec
[M::process] read 687912 sequences (100000004 bp) in 1.332 CPU sec, 1.336 real sec...
[M::mem_process_seqs] Processed 688628 reads in 79.663 CPU sec, 2.515 real sec
sorting block 39, #reads = 1074802 mem = 536877465 peak = 1048707072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285728, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (303.07, 144.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687314 sequences (100000244 bp) in 2.014 CPU sec, 2.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285538, 0, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 175, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 321)
[M::mem_pestat] mean and std.dev: (152.50, 29.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 382)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (306.13, 145.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 239, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 482)
[M::mem_pestat] mean and std.dev: (236.92, 59.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 578)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686774 sequences (100000087 bp) in 1.300 CPU sec, 1.304 real sec...
[M::mem_process_seqs] Processed 688414 reads in 134.705 CPU sec, 4.294 real sec
[M::mem_process_seqs] Processed 687912 reads in 87.325 CPU sec, 2.762 real sec
sorting block 40, #reads = 1073191 mem = 536880382 peak = 1048707072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285464, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (88, 109, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442)
[M::mem_pestat] mean and std.dev: (155.70, 84.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 560)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (306.39, 145.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686964 sequences (100000068 bp) in 2.318 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285057, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (307.74, 145.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688370 sequences (100000153 bp) in 1.330 CPU sec, 1.335 real sec...
[M::mem_process_seqs] Processed 687314 reads in 126.927 CPU sec, 4.023 real sec
sorting block 41, #reads = 1071064 mem = 536878722 peak = 1048707072
[M::mem_process_seqs] Processed 686774 reads in 80.277 CPU sec, 2.535 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285314, 0, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (112, 198, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (173.00, 54.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 289, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (309.14, 147.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 246, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (239.25, 88.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 711)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 42, #reads = 1072120 mem = 536878137 peak = 1048707072
[M::process] read 690558 sequences (100000094 bp) in 2.036 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285910, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 285, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (306.60, 148.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (117, 243, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522)
[M::mem_pestat] mean and std.dev: (188.64, 66.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] skip orientation RR
[M::process] read 690752 sequences (100000115 bp) in 1.350 CPU sec, 1.350 real sec...
[M::mem_process_seqs] Processed 686964 reads in 132.603 CPU sec, 4.221 real sec
[M::mem_process_seqs] Processed 688370 reads in 85.459 CPU sec, 2.702 real sec
sorting block 43, #reads = 1071415 mem = 536879007 peak = 1048707072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286825, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 279, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (302.83, 149.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689762 sequences (100000142 bp) in 2.341 CPU sec, 2.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287049, 0, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 189, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (168.90, 52.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (299.16, 147.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 690558 reads in 128.280 CPU sec, 4.072 real sec
[M::process] read 689214 sequences (100000129 bp) in 1.396 CPU sec, 1.404 real sec...
[M::mem_process_seqs] Processed 690752 reads in 81.399 CPU sec, 2.569 real sec
sorting block 44, #reads = 1072162 mem = 536879916 peak = 1048707072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286059, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 237, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410)
[M::mem_pestat] mean and std.dev: (200.78, 45.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 492)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (302.47, 147.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688680 sequences (100000042 bp) in 2.007 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286480, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (305.57, 149.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687132 sequences (100000166 bp) in 1.270 CPU sec, 1.272 real sec...
[M::mem_process_seqs] Processed 689762 reads in 131.835 CPU sec, 4.198 real sec
sorting block 45, #reads = 1075036 mem = 536879672 peak = 1048707072
[M::mem_process_seqs] Processed 689214 reads in 86.257 CPU sec, 2.737 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286332, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (303.57, 146.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 180, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (200.60, 105.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RR
sorting block 46, #reads = 1071437 mem = 536883520 peak = 1048707072
[M::process] read 686356 sequences (100000168 bp) in 2.345 CPU sec, 2.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285209, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (305.29, 143.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 184, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (264.22, 273.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] skip orientation RR
[M::process] read 686316 sequences (100000122 bp) in 1.340 CPU sec, 1.335 real sec...
[M::mem_process_seqs] Processed 688680 reads in 138.837 CPU sec, 4.413 real sec
[M::mem_process_seqs] Processed 687132 reads in 83.767 CPU sec, 2.657 real sec
sorting block 47, #reads = 1069375 mem = 536879243 peak = 1048707072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285252, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 217, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (161, 301)
[M::mem_pestat] mean and std.dev: (229.30, 13.21)
[M::mem_pestat] low and high boundaries for proper pairs: (133, 329)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 290, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (307.78, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685954 sequences (100000093 bp) in 1.998 CPU sec, 2.114 real sec...
[M::process] read 292898 sequences (42692265 bp) in 0.700 CPU sec, 0.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285198, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 291, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (309.06, 145.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 194, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (206.30, 103.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686356 reads in 123.731 CPU sec, 3.964 real sec
[M::mem_process_seqs] Processed 686316 reads in 78.572 CPU sec, 2.506 real sec
sorting block 48, #reads = 1071633 mem = 536878724 peak = 1048707072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284923, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 293, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (312.19, 147.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 122172, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (313.43, 149.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685954 reads in 91.750 CPU sec, 2.984 real sec
[M::mem_process_seqs] Processed 292898 reads in 40.249 CPU sec, 1.306 real sec
sorting block 49, #reads = 1072511 mem = 536881990 peak = 1048707072
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 53953106
overall: 69800075264 mem 6693.729 user 255.670 sys 593.069 real
sorting block 50, #reads = 554132 mem = 277600939 peak = 1048707072
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0002_B23KGCJLT4_1.L006\tPU:L006\tSM:NA12878_c_0002_gDNA_0002_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0002_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 593.118 sec; CPU: 6949.414 sec
uniq header count 1
merging 51 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2811595301
output reads: 54218550
bam_mem_sort: 51 calls 19.660 user 0.413 sys 20.179 real
bam_write: 51 calls 18.277 user 0.818 sys 19.263 real
execute: 1 calls 10.278 user 0.439 sys 608.060 real
merge_files: 1 calls 10.268 user 0.409 sys 14.252 real
parse_chunk: 809 calls 25.734 user 7.280 sys 33.771 real
read_chunk: 2186 calls 18.992 user 19.113 sys 180.726 real
sort_block: 1 calls 15.353 user 18.631 sys 175.097 real
write_chunk: 1375 calls 9.953 user 0.241 sys 13.572 real
overall: 2573959168 mem 557.447 user 34.679 sys 608.079 real
ls: cannot access '*.cram': No such file or directory