cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_Fr-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1791.343 CPU sec, 72.103 real sec
[M::process] read 679540 sequences (100000224 bp) in 0.896 CPU sec, 0.900 real sec...
[M::process] read 678624 sequences (100000030 bp) in 1.087 CPU sec, 1.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281115, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 303, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (321.46, 153.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678072 sequences (100000270 bp) in 1.087 CPU sec, 1.103 real sec...
[M::process] read 678358 sequences (100000056 bp) in 1.103 CPU sec, 1.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281306, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 312, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (328.22, 155.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679540 reads in 123.759 CPU sec, 3.932 real sec
[M::mem_process_seqs] Processed 678624 reads in 76.213 CPU sec, 2.445 real sec
sorting block 0, #reads = 1082830 mem = 536878508 peak = 602189824
[M::process] read 678694 sequences (100000027 bp) in 1.329 CPU sec, 1.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281949, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 319, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (334.92, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678946 sequences (100000079 bp) in 1.120 CPU sec, 1.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281373, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 314, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (332.05, 159.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281625, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 312, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (330.22, 158.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678072 reads in 173.156 CPU sec, 5.548 real sec
[M::mem_process_seqs] Processed 678358 reads in 129.396 CPU sec, 4.176 real sec
[M::mem_process_seqs] Processed 678694 reads in 141.006 CPU sec, 4.573 real sec
sorting block 1, #reads = 1082943 mem = 536883747 peak = 729591808
[M::process] read 678626 sequences (100000219 bp) in 1.564 CPU sec, 1.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281653, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 309, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (327.16, 156.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1082503 mem = 536880489 peak = 928624640
[M::process] read 678170 sequences (100000051 bp) in 1.344 CPU sec, 1.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281650, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 311, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (329.09, 157.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678946 reads in 124.571 CPU sec, 3.961 real sec
[M::mem_process_seqs] Processed 678626 reads in 79.039 CPU sec, 2.489 real sec
[M::process] read 678304 sequences (100000122 bp) in 1.358 CPU sec, 1.390 real sec...
sorting block 3, #reads = 1083098 mem = 536877711 peak = 928624640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281430, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 312, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (330.50, 158.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678692 sequences (100000010 bp) in 1.605 CPU sec, 1.689 real sec...
[M::process] read 678722 sequences (100000060 bp) in 1.180 CPU sec, 1.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281223, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 308, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (327.81, 158.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678170 reads in 123.563 CPU sec, 3.935 real sec
[M::mem_process_seqs] Processed 678304 reads in 77.999 CPU sec, 2.478 real sec
sorting block 4, #reads = 1082732 mem = 536877445 peak = 928624640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281396, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 308, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (328.26, 158.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678404 sequences (100000096 bp) in 1.660 CPU sec, 1.772 real sec...
[M::process] read 677650 sequences (100000089 bp) in 1.200 CPU sec, 1.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281526, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 314, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (329.73, 157.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678692 reads in 122.373 CPU sec, 3.895 real sec
[M::mem_process_seqs] Processed 678722 reads in 76.816 CPU sec, 2.439 real sec
sorting block 5, #reads = 1082522 mem = 536880937 peak = 928624640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281541, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (333.14, 158.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678314 sequences (100000053 bp) in 1.793 CPU sec, 1.874 real sec...
[M::process] read 678968 sequences (100000195 bp) in 1.220 CPU sec, 1.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281076, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 324, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (339.31, 161.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678404 reads in 123.000 CPU sec, 3.911 real sec
sorting block 6, #reads = 1083648 mem = 536877563 peak = 928624640
[M::mem_process_seqs] Processed 677650 reads in 77.768 CPU sec, 2.471 real sec
sorting block 7, #reads = 1081589 mem = 536880039 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281828, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 314, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (332.47, 160.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678724 sequences (100000099 bp) in 1.704 CPU sec, 1.818 real sec...
[M::process] read 678774 sequences (100000150 bp) in 1.280 CPU sec, 1.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282069, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 308, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (327.12, 157.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678314 reads in 124.127 CPU sec, 3.947 real sec
[M::mem_process_seqs] Processed 678968 reads in 78.430 CPU sec, 2.488 real sec
sorting block 8, #reads = 1083047 mem = 536880550 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281212, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 310, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (329.14, 158.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678994 sequences (100000100 bp) in 1.962 CPU sec, 2.042 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281715, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 308, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (327.84, 157.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678636 sequences (100000007 bp) in 1.310 CPU sec, 1.311 real sec...
[M::mem_process_seqs] Processed 678724 reads in 124.359 CPU sec, 3.947 real sec
[M::mem_process_seqs] Processed 678774 reads in 78.961 CPU sec, 2.503 real sec
sorting block 9, #reads = 1083179 mem = 536878128 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281588, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 302, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (324.99, 157.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679056 sequences (100000190 bp) in 1.752 CPU sec, 1.834 real sec...
[M::process] read 678368 sequences (100000263 bp) in 1.290 CPU sec, 1.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281728, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 305, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (327.38, 159.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678994 reads in 125.112 CPU sec, 3.979 real sec
[M::mem_process_seqs] Processed 678636 reads in 79.034 CPU sec, 2.509 real sec
sorting block 10, #reads = 1083140 mem = 536878855 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282293, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 309, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (326.06, 155.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677470 sequences (100000061 bp) in 2.152 CPU sec, 2.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282267, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (332.38, 158.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677502 sequences (100000008 bp) in 1.350 CPU sec, 1.350 real sec...
[M::mem_process_seqs] Processed 679056 reads in 124.980 CPU sec, 3.964 real sec
sorting block 11, #reads = 1081717 mem = 536881272 peak = 934748160
[M::mem_process_seqs] Processed 678368 reads in 78.768 CPU sec, 2.491 real sec
sorting block 12, #reads = 1083013 mem = 536880679 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281071, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 325, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (340.33, 162.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678522 sequences (100000033 bp) in 1.814 CPU sec, 1.912 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281386, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 325, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (340.83, 163.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678244 sequences (100000087 bp) in 1.360 CPU sec, 1.355 real sec...
[M::mem_process_seqs] Processed 677470 reads in 125.630 CPU sec, 3.999 real sec
[M::mem_process_seqs] Processed 677502 reads in 79.603 CPU sec, 2.517 real sec
sorting block 13, #reads = 1081982 mem = 536878852 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281537, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 311, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (329.17, 158.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678234 sequences (100000049 bp) in 2.164 CPU sec, 2.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281162, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (332.62, 159.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678624 sequences (100000175 bp) in 1.370 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 678522 reads in 127.097 CPU sec, 4.034 real sec
[M::mem_process_seqs] Processed 678244 reads in 80.894 CPU sec, 2.557 real sec
sorting block 14, #reads = 1083757 mem = 536879452 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281288, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 311, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (331.24, 160.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678076 sequences (100000178 bp) in 1.899 CPU sec, 1.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281350, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 304, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (326.26, 158.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678234 reads in 127.583 CPU sec, 4.062 real sec
[M::process] read 678542 sequences (100000018 bp) in 1.410 CPU sec, 1.407 real sec...
[M::mem_process_seqs] Processed 678624 reads in 81.286 CPU sec, 2.570 real sec
sorting block 15, #reads = 1082389 mem = 536877882 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281328, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 308, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (330.21, 160.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678474 sequences (100000018 bp) in 2.196 CPU sec, 2.310 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281603, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 307, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (327.99, 159.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678144 sequences (100000103 bp) in 1.334 CPU sec, 1.345 real sec...
[M::mem_process_seqs] Processed 678076 reads in 126.823 CPU sec, 4.027 real sec
sorting block 16, #reads = 1082705 mem = 536878978 peak = 934748160
[M::mem_process_seqs] Processed 678542 reads in 80.983 CPU sec, 2.565 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281146, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 313, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (330.57, 158.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1082449 mem = 536877243 peak = 934748160
[M::process] read 677150 sequences (100000117 bp) in 1.972 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281616, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 319, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (335.75, 161.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678474 reads in 126.109 CPU sec, 4.031 real sec
[M::mem_process_seqs] Processed 678144 reads in 81.697 CPU sec, 2.583 real sec
[M::process] read 677580 sequences (100000181 bp) in 1.510 CPU sec, 1.541 real sec...
sorting block 18, #reads = 1082592 mem = 536881314 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281229, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 325, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (341.24, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678572 sequences (100000163 bp) in 2.198 CPU sec, 2.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281548, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 319, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (336.56, 162.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677150 reads in 126.217 CPU sec, 4.017 real sec
[M::process] read 678262 sequences (100000276 bp) in 1.455 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 677580 reads in 80.461 CPU sec, 2.540 real sec
sorting block 19, #reads = 1081084 mem = 536880301 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281337, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 309, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (328.97, 159.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678860 sequences (100000126 bp) in 1.950 CPU sec, 2.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281870, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 308, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (328.96, 159.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678832 sequences (100000295 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_process_seqs] Processed 678572 reads in 127.537 CPU sec, 4.064 real sec
sorting block 20, #reads = 1082238 mem = 536877680 peak = 934748160
[M::mem_process_seqs] Processed 678262 reads in 81.147 CPU sec, 2.566 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281777, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 302, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (324.16, 157.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1082054 mem = 536876342 peak = 934748160
[M::process] read 678382 sequences (100000289 bp) in 2.154 CPU sec, 2.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281437, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 302, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (324.79, 158.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678860 reads in 128.034 CPU sec, 4.077 real sec
[M::mem_process_seqs] Processed 678832 reads in 81.490 CPU sec, 2.574 real sec
[M::process] read 680454 sequences (100000085 bp) in 1.556 CPU sec, 1.592 real sec...
sorting block 22, #reads = 1082862 mem = 536881153 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281391, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 306, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (328.97, 161.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679180 sequences (100000111 bp) in 1.830 CPU sec, 1.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282372, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 296, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (318.97, 156.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678534 sequences (100000239 bp) in 1.340 CPU sec, 1.347 real sec...
[M::mem_process_seqs] Processed 678382 reads in 129.927 CPU sec, 4.147 real sec
[M::mem_process_seqs] Processed 680454 reads in 83.457 CPU sec, 2.645 real sec
sorting block 23, #reads = 1082435 mem = 536876929 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282030, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 310, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (330.01, 160.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679102 sequences (100000171 bp) in 2.456 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281817, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (334.55, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679180 reads in 129.692 CPU sec, 4.122 real sec
[M::mem_process_seqs] Processed 678534 reads in 82.417 CPU sec, 2.600 real sec
[M::process] read 679532 sequences (100000061 bp) in 1.513 CPU sec, 1.547 real sec...
sorting block 24, #reads = 1082762 mem = 536879766 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281930, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 309, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (329.40, 159.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679614 sequences (100000115 bp) in 1.813 CPU sec, 1.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282003, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 301, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (322.69, 156.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679500 sequences (100000153 bp) in 1.350 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 679102 reads in 131.671 CPU sec, 4.198 real sec
sorting block 25, #reads = 1082118 mem = 536878996 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281967, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 298, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (316.77, 150.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679164 sequences (100000220 bp) in 1.755 CPU sec, 1.758 real sec...
[M::mem_process_seqs] Processed 679614 reads in 71.188 CPU sec, 2.248 real sec
sorting block 26, #reads = 1082880 mem = 536879926 peak = 934748160
[M::mem_process_seqs] Processed 679532 reads in 180.568 CPU sec, 5.733 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281867, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 299, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (317.81, 150.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679004 sequences (100000193 bp) in 1.959 CPU sec, 2.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281498, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 299, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (317.95, 150.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679500 reads in 144.622 CPU sec, 4.593 real sec
[M::process] read 680032 sequences (100000012 bp) in 1.439 CPU sec, 1.449 real sec...
[M::mem_process_seqs] Processed 679164 reads in 84.870 CPU sec, 2.682 real sec
sorting block 27, #reads = 1082167 mem = 536879479 peak = 934748160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281867, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 302, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (320.32, 151.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679614 sequences (100000271 bp) in 2.225 CPU sec, 2.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282161, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 298, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (320.07, 155.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678494 sequences (100000081 bp) in 1.330 CPU sec, 1.332 real sec...
[M::mem_process_seqs] Processed 679004 reads in 133.181 CPU sec, 4.233 real sec
sorting block 28, #reads = 1080575 mem = 536879039 peak = 934748160
[M::mem_process_seqs] Processed 680032 reads in 85.050 CPU sec, 2.696 real sec
sorting block 29, #reads = 1080955 mem = 536878715 peak = 981897216
[M::process] read 678616 sequences (100000005 bp) in 1.951 CPU sec, 2.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281798, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 316, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (331.45, 157.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679922 sequences (100000053 bp) in 1.360 CPU sec, 1.368 real sec...
[M::mem_process_seqs] Processed 678494 reads in 74.981 CPU sec, 2.374 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282074, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 305, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (324.33, 155.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281810, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 309, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (327.34, 156.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679556 sequences (100000276 bp) in 1.369 CPU sec, 1.375 real sec...
[M::mem_process_seqs] Processed 679614 reads in 201.870 CPU sec, 6.425 real sec
sorting block 30, #reads = 1081683 mem = 536878401 peak = 981897216
[M::mem_process_seqs] Processed 678616 reads in 94.880 CPU sec, 3.035 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282451, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 297, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (316.97, 151.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1080833 mem = 536879492 peak = 981897216
[M::process] read 679222 sequences (100000094 bp) in 2.114 CPU sec, 2.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282266, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 299, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (318.45, 151.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678966 sequences (100000183 bp) in 1.310 CPU sec, 1.313 real sec...
[M::mem_process_seqs] Processed 679922 reads in 131.518 CPU sec, 4.182 real sec
[M::mem_process_seqs] Processed 679556 reads in 82.917 CPU sec, 2.628 real sec
sorting block 32, #reads = 1081507 mem = 536879397 peak = 981897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282666, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 302, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (320.88, 152.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678902 sequences (100000035 bp) in 2.073 CPU sec, 2.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282005, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 304, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (322.30, 153.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679670 sequences (100000031 bp) in 1.370 CPU sec, 1.368 real sec...
[M::mem_process_seqs] Processed 679222 reads in 130.168 CPU sec, 4.133 real sec
[M::mem_process_seqs] Processed 678966 reads in 82.681 CPU sec, 2.623 real sec
sorting block 33, #reads = 1081368 mem = 536880846 peak = 981897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281398, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 302, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (322.58, 155.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679178 sequences (100000164 bp) in 2.158 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282162, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 301, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (323.00, 157.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677952 sequences (100000182 bp) in 1.320 CPU sec, 1.317 real sec...
[M::mem_process_seqs] Processed 678902 reads in 134.034 CPU sec, 4.260 real sec
[M::mem_process_seqs] Processed 679670 reads in 86.459 CPU sec, 2.741 real sec
sorting block 34, #reads = 1080456 mem = 536881459 peak = 981897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282296, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 308, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (325.03, 154.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677220 sequences (100000180 bp) in 1.804 CPU sec, 1.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281762, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 319, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (332.99, 156.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678682 sequences (100000227 bp) in 1.350 CPU sec, 1.348 real sec...
[M::mem_process_seqs] Processed 679178 reads in 126.882 CPU sec, 4.037 real sec
sorting block 35, #reads = 1078749 mem = 536879842 peak = 981897216
[M::mem_process_seqs] Processed 677952 reads in 79.429 CPU sec, 2.513 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280976, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (335.04, 156.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1081452 mem = 536880254 peak = 981897216
[M::process] read 679370 sequences (100000119 bp) in 2.119 CPU sec, 2.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281623, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 305, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (322.36, 152.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679068 sequences (100000177 bp) in 1.350 CPU sec, 1.348 real sec...
[M::mem_process_seqs] Processed 677220 reads in 126.268 CPU sec, 4.010 real sec
[M::mem_process_seqs] Processed 678682 reads in 80.121 CPU sec, 2.533 real sec
sorting block 37, #reads = 1081187 mem = 536879451 peak = 981897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282027, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 300, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (318.25, 150.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678358 sequences (100000244 bp) in 1.890 CPU sec, 1.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281733, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 302, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (319.33, 150.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678526 sequences (100000062 bp) in 1.360 CPU sec, 1.361 real sec...
[M::mem_process_seqs] Processed 679370 reads in 128.589 CPU sec, 4.093 real sec
[M::mem_process_seqs] Processed 679068 reads in 81.704 CPU sec, 2.588 real sec
sorting block 38, #reads = 1082044 mem = 536878055 peak = 981897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282214, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 305, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (322.92, 152.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678706 sequences (100000288 bp) in 2.245 CPU sec, 2.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281444, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 304, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (323.29, 154.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680418 sequences (100000104 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_process_seqs] Processed 678358 reads in 131.242 CPU sec, 4.166 real sec
[M::mem_process_seqs] Processed 678526 reads in 84.691 CPU sec, 2.681 real sec
sorting block 39, #reads = 1081724 mem = 536878243 peak = 981897216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281043, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 305, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (325.43, 156.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678704 sequences (100000078 bp) in 1.942 CPU sec, 2.044 real sec...
[M::process] read 677998 sequences (100000266 bp) in 1.330 CPU sec, 1.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282541, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 298, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (322.67, 159.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678706 reads in 137.862 CPU sec, 4.390 real sec
sorting block 40, #reads = 1079372 mem = 536877870 peak = 981897216
[M::mem_process_seqs] Processed 680418 reads in 83.426 CPU sec, 2.646 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282022, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (334.08, 161.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1078349 mem = 536880164 peak = 1019969536
[M::process] read 678632 sequences (100000272 bp) in 2.259 CPU sec, 2.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281875, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (340.91, 164.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678890 sequences (100000169 bp) in 1.350 CPU sec, 1.347 real sec...
[M::mem_process_seqs] Processed 678704 reads in 130.232 CPU sec, 4.136 real sec
[M::mem_process_seqs] Processed 677998 reads in 82.685 CPU sec, 2.614 real sec
sorting block 42, #reads = 1079799 mem = 536879196 peak = 1019969536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282237, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 311, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (330.05, 159.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678678 sequences (100000242 bp) in 1.941 CPU sec, 2.042 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282153, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 306, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (325.83, 157.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679030 sequences (100000012 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_process_seqs] Processed 678632 reads in 130.272 CPU sec, 4.145 real sec
[M::mem_process_seqs] Processed 678890 reads in 82.074 CPU sec, 2.597 real sec
sorting block 43, #reads = 1080926 mem = 536879879 peak = 1019969536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281674, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 307, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (327.14, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678790 sequences (100000228 bp) in 1.948 CPU sec, 2.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281750, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 301, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (322.98, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678406 sequences (100000137 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 678678 reads in 132.004 CPU sec, 4.215 real sec
sorting block 44, #reads = 1081615 mem = 536880283 peak = 1019969536
[M::mem_process_seqs] Processed 679030 reads in 83.565 CPU sec, 2.645 real sec
[M::process] read 81030 sequences (11935253 bp) in 0.530 CPU sec, 0.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281574, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 300, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (322.40, 155.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281571, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 303, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (324.25, 155.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 33558, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 303, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (325.33, 157.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678790 reads in 134.430 CPU sec, 4.360 real sec
sorting block 45, #reads = 1080846 mem = 536877002 peak = 1019969536
[M::mem_process_seqs] Processed 678406 reads in 87.300 CPU sec, 2.829 real sec
[M::mem_process_seqs] Processed 81030 reads in 88.852 CPU sec, 2.885 real sec
sorting block 46, #reads = 1080860 mem = 536879295 peak = 1147023360
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 50984820
overall: 69725818880 mem 6691.648 user 348.901 sys 247.370 real
sorting block 47, #reads = 349614 mem = 173708520 peak = 1147023360
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.659_Fr-N1-BDNA-1_B23KGCJLT4_1.L005\tPU:L005\tSM:659_Fr-N1-BDNA-1_B23KGCJLT4_1\tLB:659_Fr-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_Fr-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 659_Fr-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 247.428 sec; CPU: 7040.573 sec
uniq header count 1
merging 48 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2654776823
output reads: 51196349
bam_mem_sort: 48 calls 20.236 user 0.444 sys 20.805 real
bam_write: 48 calls 23.603 user 1.156 sys 24.971 real
execute: 1 calls 9.996 user 0.638 sys 261.694 real
merge_files: 1 calls 9.975 user 0.619 sys 13.861 real
parse_chunk: 758 calls 25.194 user 6.505 sys 32.226 real
read_chunk: 2043 calls 17.512 user 16.879 sys 175.284 real
sort_block: 1 calls 14.814 user 16.353 sys 170.876 real
write_chunk: 1283 calls 9.618 user 0.492 sys 13.174 real
overall: 2499182592 mem 535.370 user 34.415 sys 261.717 real
ls: cannot access '*.cram': No such file or directory