cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_0W2-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1810.195 CPU sec, 75.146 real sec
[M::process] read 678208 sequences (100000006 bp) in 0.904 CPU sec, 0.905 real sec...
[M::process] read 678686 sequences (100000147 bp) in 1.120 CPU sec, 1.128 real sec...
[M::process] read 678160 sequences (100000171 bp) in 1.123 CPU sec, 1.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279516, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 372, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (383.29, 179.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677484 sequences (100000194 bp) in 1.082 CPU sec, 1.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279800, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 377, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (390.38, 185.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1371)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279813, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 366, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (379.20, 178.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678208 reads in 212.913 CPU sec, 6.862 real sec
[M::mem_process_seqs] Processed 678160 reads in 144.722 CPU sec, 4.703 real sec
[M::mem_process_seqs] Processed 678686 reads in 157.170 CPU sec, 5.122 real sec
sorting block 0, #reads = 1078393 mem = 536879627 peak = 601812992
[M::process] read 676966 sequences (100000165 bp) in 1.441 CPU sec, 1.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278975, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 380, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081)
[M::mem_pestat] mean and std.dev: (393.23, 184.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677212 sequences (100000227 bp) in 1.163 CPU sec, 1.174 real sec...
[M::process] read 677212 sequences (100000198 bp) in 1.030 CPU sec, 1.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278170, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 380, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (392.85, 182.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1353)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677484 reads in 140.143 CPU sec, 4.459 real sec
sorting block 1, #reads = 1077799 mem = 536878289 peak = 835936256
[M::mem_process_seqs] Processed 676966 reads in 88.399 CPU sec, 2.808 real sec
sorting block 2, #reads = 1077268 mem = 536878589 peak = 973074432
[M::process] read 677808 sequences (100000156 bp) in 1.453 CPU sec, 1.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278711, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 375, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080)
[M::mem_pestat] mean and std.dev: (390.66, 184.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677764 sequences (100000231 bp) in 1.113 CPU sec, 1.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278828, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 377, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (393.11, 187.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1373)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279830, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (390.01, 180.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677212 reads in 193.004 CPU sec, 6.182 real sec
[M::mem_process_seqs] Processed 677212 reads in 141.104 CPU sec, 4.542 real sec
[M::mem_process_seqs] Processed 677808 reads in 153.294 CPU sec, 4.955 real sec
sorting block 3, #reads = 1077323 mem = 536880676 peak = 973074432
[M::process] read 677740 sequences (100000074 bp) in 1.793 CPU sec, 1.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279831, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 376, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063)
[M::mem_pestat] mean and std.dev: (388.31, 181.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1337)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1077475 mem = 536882006 peak = 973074432
[M::process] read 677028 sequences (100000235 bp) in 1.401 CPU sec, 1.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279804, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 381, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (393.17, 184.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677764 reads in 134.645 CPU sec, 4.295 real sec
[M::process] read 677088 sequences (100000156 bp) in 1.121 CPU sec, 1.139 real sec...
[M::mem_process_seqs] Processed 677740 reads in 87.064 CPU sec, 2.751 real sec
sorting block 5, #reads = 1077188 mem = 536878132 peak = 973074432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279207, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 384, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (396.74, 185.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1371)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677190 sequences (100000100 bp) in 1.546 CPU sec, 1.638 real sec...
[M::process] read 676812 sequences (100000014 bp) in 1.182 CPU sec, 1.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279728, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 382, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082)
[M::mem_pestat] mean and std.dev: (394.47, 184.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1361)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677028 reads in 136.143 CPU sec, 4.352 real sec
[M::mem_process_seqs] Processed 677088 reads in 85.758 CPU sec, 2.750 real sec
sorting block 6, #reads = 1077917 mem = 536878992 peak = 973074432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279374, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 380, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095)
[M::mem_pestat] mean and std.dev: (395.70, 188.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1379)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677706 sequences (100000228 bp) in 1.715 CPU sec, 1.809 real sec...
[M::process] read 677898 sequences (100000179 bp) in 1.160 CPU sec, 1.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279315, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 382, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (397.48, 188.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1381)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677190 reads in 135.456 CPU sec, 4.320 real sec
[M::mem_process_seqs] Processed 676812 reads in 85.426 CPU sec, 2.728 real sec
sorting block 7, #reads = 1076895 mem = 536878228 peak = 973074432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279749, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 380, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (392.45, 184.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677574 sequences (100000041 bp) in 1.949 CPU sec, 2.080 real sec...
[M::process] read 676846 sequences (100000066 bp) in 1.180 CPU sec, 1.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279762, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 379, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070)
[M::mem_pestat] mean and std.dev: (390.95, 184.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1346)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677706 reads in 130.156 CPU sec, 4.146 real sec
sorting block 8, #reads = 1077056 mem = 536879244 peak = 973074432
[M::mem_process_seqs] Processed 677898 reads in 83.423 CPU sec, 2.654 real sec
sorting block 9, #reads = 1077720 mem = 536879621 peak = 987566080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279208, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 382, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089)
[M::mem_pestat] mean and std.dev: (394.37, 185.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1371)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677282 sequences (100000121 bp) in 1.894 CPU sec, 2.017 real sec...
[M::process] read 677034 sequences (100000212 bp) in 1.210 CPU sec, 1.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279219, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 386, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094)
[M::mem_pestat] mean and std.dev: (399.27, 187.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1376)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677574 reads in 133.652 CPU sec, 4.258 real sec
[M::mem_process_seqs] Processed 676846 reads in 85.229 CPU sec, 2.713 real sec
sorting block 10, #reads = 1077272 mem = 536878702 peak = 987566080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279761, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 383, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105)
[M::mem_pestat] mean and std.dev: (398.02, 189.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1392)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676626 sequences (100000022 bp) in 1.973 CPU sec, 2.115 real sec...
[M::process] read 678024 sequences (100000006 bp) in 1.210 CPU sec, 1.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279482, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 380, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104)
[M::mem_pestat] mean and std.dev: (396.89, 189.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1391)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677282 reads in 133.980 CPU sec, 4.270 real sec
[M::mem_process_seqs] Processed 677034 reads in 84.865 CPU sec, 2.704 real sec
sorting block 11, #reads = 1077615 mem = 536877808 peak = 987566080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279272, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 386, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109)
[M::mem_pestat] mean and std.dev: (401.97, 190.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1396)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677956 sequences (100000136 bp) in 2.034 CPU sec, 2.194 real sec...
[M::process] read 677758 sequences (100000037 bp) in 1.240 CPU sec, 1.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279829, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 379, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (394.62, 188.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1385)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676626 reads in 133.241 CPU sec, 4.244 real sec
[M::mem_process_seqs] Processed 678024 reads in 85.277 CPU sec, 2.714 real sec
sorting block 12, #reads = 1077411 mem = 536879902 peak = 987566080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279384, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 374, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (388.46, 183.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677504 sequences (100000025 bp) in 2.044 CPU sec, 2.191 real sec...
[M::process] read 677336 sequences (100000267 bp) in 1.240 CPU sec, 1.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279503, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 376, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (390.51, 185.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677956 reads in 136.673 CPU sec, 4.356 real sec
sorting block 13, #reads = 1077823 mem = 536878402 peak = 987566080
[M::mem_process_seqs] Processed 677758 reads in 87.421 CPU sec, 2.782 real sec
sorting block 14, #reads = 1077521 mem = 536882213 peak = 996585472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279768, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 377, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098)
[M::mem_pestat] mean and std.dev: (392.95, 187.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1384)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677434 sequences (100000235 bp) in 1.982 CPU sec, 2.101 real sec...
[M::process] read 676662 sequences (100000029 bp) in 1.240 CPU sec, 1.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278969, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 377, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087)
[M::mem_pestat] mean and std.dev: (392.14, 185.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1369)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677504 reads in 139.009 CPU sec, 4.431 real sec
[M::mem_process_seqs] Processed 677336 reads in 87.883 CPU sec, 2.799 real sec
sorting block 15, #reads = 1076500 mem = 536878942 peak = 996585472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279377, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 376, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087)
[M::mem_pestat] mean and std.dev: (392.12, 186.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1369)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679136 sequences (100000177 bp) in 1.970 CPU sec, 2.088 real sec...
[M::process] read 679426 sequences (100000052 bp) in 1.240 CPU sec, 1.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279060, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 383, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109)
[M::mem_pestat] mean and std.dev: (399.64, 190.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1397)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677434 reads in 136.540 CPU sec, 4.350 real sec
[M::mem_process_seqs] Processed 676662 reads in 86.352 CPU sec, 2.750 real sec
sorting block 16, #reads = 1077286 mem = 536882549 peak = 996585472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280113, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 369, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (382.63, 182.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1345)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679230 sequences (100000209 bp) in 2.094 CPU sec, 2.216 real sec...
[M::process] read 678988 sequences (100000160 bp) in 1.200 CPU sec, 1.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279374, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 360, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (373.31, 176.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679136 reads in 140.449 CPU sec, 4.477 real sec
[M::mem_process_seqs] Processed 679426 reads in 87.639 CPU sec, 2.791 real sec
sorting block 17, #reads = 1076497 mem = 536882523 peak = 996585472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279155, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 363, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (376.08, 178.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678428 sequences (100000256 bp) in 2.029 CPU sec, 2.142 real sec...
[M::process] read 678300 sequences (100000128 bp) in 1.230 CPU sec, 1.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279132, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 359, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (371.96, 174.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679230 reads in 143.800 CPU sec, 4.584 real sec
sorting block 18, #reads = 1078476 mem = 536877692 peak = 996585472
[M::mem_process_seqs] Processed 678988 reads in 89.738 CPU sec, 2.861 real sec
sorting block 19, #reads = 1078775 mem = 536882270 peak = 996585472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279271, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 358, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (371.21, 173.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677752 sequences (100000300 bp) in 2.069 CPU sec, 2.193 real sec...
[M::process] read 679160 sequences (100000197 bp) in 1.190 CPU sec, 1.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279897, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 358, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (370.98, 172.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678428 reads in 140.615 CPU sec, 4.481 real sec
[M::mem_process_seqs] Processed 678300 reads in 87.728 CPU sec, 2.794 real sec
sorting block 20, #reads = 1077682 mem = 536879300 peak = 996585472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279344, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 365, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (381.55, 180.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679356 sequences (100000030 bp) in 2.084 CPU sec, 2.197 real sec...
[M::process] read 679146 sequences (100000153 bp) in 1.210 CPU sec, 1.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280173, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 363, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (377.20, 179.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677752 reads in 136.959 CPU sec, 4.362 real sec
[M::mem_process_seqs] Processed 679160 reads in 84.749 CPU sec, 2.698 real sec
sorting block 21, #reads = 1076446 mem = 536877613 peak = 996585472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280122, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 356, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (370.98, 175.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678866 sequences (100000133 bp) in 2.088 CPU sec, 2.218 real sec...
[M::process] read 678284 sequences (100000209 bp) in 1.190 CPU sec, 1.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279970, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 353, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (367.51, 173.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679356 reads in 136.384 CPU sec, 4.347 real sec
sorting block 22, #reads = 1078848 mem = 536883384 peak = 996585472
[M::mem_process_seqs] Processed 679146 reads in 86.220 CPU sec, 2.744 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279753, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 359, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (373.48, 176.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677700 sequences (100000265 bp) in 2.152 CPU sec, 2.296 real sec...
[M::process] read 677230 sequences (100000187 bp) in 1.240 CPU sec, 1.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279597, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 363, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (377.04, 177.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678866 reads in 139.226 CPU sec, 4.445 real sec
sorting block 23, #reads = 1080090 mem = 536879965 peak = 996585472
[M::mem_process_seqs] Processed 678284 reads in 87.571 CPU sec, 2.791 real sec
sorting block 24, #reads = 1078553 mem = 536879137 peak = 1003540480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279587, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 372, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (385.68, 180.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1320)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678874 sequences (100000199 bp) in 2.110 CPU sec, 2.247 real sec...
[M::process] read 678914 sequences (100000115 bp) in 1.200 CPU sec, 1.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279407, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 374, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (390.26, 185.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1352)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677700 reads in 137.778 CPU sec, 4.393 real sec
[M::mem_process_seqs] Processed 677230 reads in 87.766 CPU sec, 2.792 real sec
sorting block 25, #reads = 1078143 mem = 536879746 peak = 1003540480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280028, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 366, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (381.85, 183.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1322)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678762 sequences (100000271 bp) in 2.126 CPU sec, 2.254 real sec...
[M::process] read 678282 sequences (100000232 bp) in 1.230 CPU sec, 1.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280059, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 362, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (376.85, 179.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678874 reads in 134.764 CPU sec, 4.296 real sec
[M::mem_process_seqs] Processed 678914 reads in 84.677 CPU sec, 2.696 real sec
sorting block 26, #reads = 1076246 mem = 536877121 peak = 1003540480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279505, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 360, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (375.13, 179.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678190 sequences (100000108 bp) in 2.129 CPU sec, 2.280 real sec...
[M::process] read 677626 sequences (100000231 bp) in 1.230 CPU sec, 1.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280308, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 361, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (376.70, 178.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678762 reads in 134.936 CPU sec, 4.301 real sec
sorting block 27, #reads = 1078703 mem = 536879096 peak = 1003540480
[M::mem_process_seqs] Processed 678282 reads in 84.986 CPU sec, 2.704 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279886, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 363, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (376.90, 177.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 28, #reads = 1079127 mem = 536880641 peak = 1003540480
[M::process] read 677396 sequences (100000009 bp) in 2.241 CPU sec, 2.379 real sec...
[M::process] read 679076 sequences (100000191 bp) in 1.250 CPU sec, 1.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280073, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 369, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (383.94, 180.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678190 reads in 138.658 CPU sec, 4.417 real sec
[M::mem_process_seqs] Processed 677626 reads in 89.026 CPU sec, 2.834 real sec
sorting block 29, #reads = 1076803 mem = 536877548 peak = 1003540480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280288, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 373, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (389.21, 184.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679058 sequences (100000197 bp) in 2.098 CPU sec, 2.214 real sec...
[M::process] read 678780 sequences (100000102 bp) in 1.210 CPU sec, 1.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279749, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 372, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105)
[M::mem_pestat] mean and std.dev: (389.01, 190.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1396)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677396 reads in 140.804 CPU sec, 4.492 real sec
[M::mem_process_seqs] Processed 679076 reads in 88.920 CPU sec, 2.836 real sec
sorting block 30, #reads = 1075369 mem = 536881370 peak = 1003540480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279893, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 370, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079)
[M::mem_pestat] mean and std.dev: (384.39, 184.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1361)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678084 sequences (100000247 bp) in 2.100 CPU sec, 2.209 real sec...
[M::process] read 677692 sequences (100000032 bp) in 1.250 CPU sec, 1.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279623, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 368, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (383.03, 182.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1346)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679058 reads in 139.074 CPU sec, 4.440 real sec
[M::mem_process_seqs] Processed 678780 reads in 87.448 CPU sec, 2.787 real sec
sorting block 31, #reads = 1078107 mem = 536880931 peak = 1003540480
[M::process] read 677218 sequences (100000188 bp) in 2.182 CPU sec, 2.332 real sec...
[M::process] read 677088 sequences (100000000 bp) in 1.230 CPU sec, 1.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278878, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 369, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (383.95, 180.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677692 reads in 82.710 CPU sec, 2.624 real sec
sorting block 32, #reads = 1077774 mem = 536879390 peak = 1003540480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279565, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 371, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (385.08, 181.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1341)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (399, 775, 1116)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2550)
[M::mem_pestat] mean and std.dev: (765.18, 366.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3267)
[M::mem_pestat] skip orientation RR
[M::process] read 63940 sequences (9431247 bp) in 0.469 CPU sec, 0.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278961, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 369, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (384.06, 180.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678084 reads in 218.656 CPU sec, 6.999 real sec
[M::mem_process_seqs] Processed 677218 reads in 102.471 CPU sec, 3.313 real sec
sorting block 33, #reads = 1076232 mem = 536879005 peak = 1003540480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279264, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 374, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077)
[M::mem_pestat] mean and std.dev: (390.89, 185.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1356)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 26410, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 373, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092)
[M::mem_pestat] mean and std.dev: (389.86, 186.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1377)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677088 reads in 80.834 CPU sec, 2.633 real sec
[M::mem_process_seqs] Processed 63940 reads in 19.838 CPU sec, 0.650 real sec
sorting block 34, #reads = 1076011 mem = 536877509 peak = 1069473792
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 38028980
overall: 69727141888 mem 5695.887 user 333.779 sys 217.049 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.1029_0W2-N1-BDNA-1_B23KGCJLT4_1.L001\tPU:L001\tSM:1029_0W2-N1-BDNA-1_B23KGCJLT4_1\tLB:1029_0W2-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_0W2-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 1029_0W2-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 217.085 sec; CPU: 6029.669 sec
sorting block 35, #reads = 466722 mem = 232839234 peak = 1069473792
uniq header count 1
merging 36 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 1979039352
output reads: 38181066
bam_mem_sort: 36 calls 14.751 user 0.194 sys 15.033 real
bam_write: 36 calls 17.361 user 0.507 sys 18.044 real
execute: 1 calls 6.865 user 0.427 sys 227.658 real
merge_files: 1 calls 6.847 user 0.408 sys 10.127 real
parse_chunk: 567 calls 17.831 user 4.791 sys 22.938 real
read_chunk: 1530 calls 12.857 user 13.948 sys 138.148 real
sort_block: 1 calls 10.828 user 13.482 sys 134.598 real
write_chunk: 961 calls 6.551 user 0.265 sys 9.524 real
overall: 2349301760 mem 387.546 user 26.054 sys 227.678 real
ls: cannot access '*.cram': No such file or directory