.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1029_0I-N1-BDNA-1_B23KGCJLT4_1;L008;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f8/2f2dc230fd274e695992c37a3f8211/.command.err
Size: 48.8 KB
Task: DAQ:SENTIEON_BWAMEM (1029_0I-N1-BDNA-1_B23KGCJLT4_1;L008;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_0I-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1643.022 CPU sec, 296.884 real sec
[M::process] read 685826 sequences (100000039 bp) in 0.911 CPU sec, 0.916 real sec...
[M::process] read 684546 sequences (100000032 bp) in 1.138 CPU sec, 1.170 real sec...
[M::process] read 683548 sequences (100000283 bp) in 1.163 CPU sec, 1.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283445, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 301, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (323.27, 158.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683542 sequences (100000269 bp) in 1.097 CPU sec, 1.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283217, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (329.93, 161.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283523, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (335.70, 163.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685826 reads in 205.595 CPU sec, 6.587 real sec
[M::mem_process_seqs] Processed 684546 reads in 131.715 CPU sec, 4.239 real sec
[M::mem_process_seqs] Processed 683548 reads in 142.959 CPU sec, 4.621 real sec
sorting block 0, #reads = 1086633 mem = 536877567 peak = 589631488
[M::process] read 682600 sequences (100000284 bp) in 1.553 CPU sec, 1.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282414, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (338.58, 166.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682300 sequences (100000210 bp) in 1.189 CPU sec, 1.204 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282256, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (340.80, 165.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681768 sequences (100000042 bp) in 1.030 CPU sec, 1.032 real sec...
[M::mem_process_seqs] Processed 683542 reads in 124.774 CPU sec, 3.969 real sec
sorting block 1, #reads = 1085923 mem = 536881423 peak = 861630464
[M::mem_process_seqs] Processed 682600 reads in 78.697 CPU sec, 2.492 real sec
sorting block 2, #reads = 1085474 mem = 536878391 peak = 913330176
[M::process] read 684670 sequences (100000271 bp) in 1.474 CPU sec, 1.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281947, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (338.95, 164.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685266 sequences (100000204 bp) in 1.140 CPU sec, 1.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282493, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 321, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (342.85, 166.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682300 reads in 124.053 CPU sec, 3.958 real sec
[M::mem_process_seqs] Processed 681768 reads in 78.190 CPU sec, 2.502 real sec
sorting block 3, #reads = 1084894 mem = 536879065 peak = 913330176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284081, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 310, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (332.31, 163.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684396 sequences (100000142 bp) in 1.664 CPU sec, 1.758 real sec...
[M::process] read 683808 sequences (100000014 bp) in 1.140 CPU sec, 1.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283940, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 309, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (330.35, 162.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684670 reads in 123.869 CPU sec, 3.954 real sec
[M::mem_process_seqs] Processed 685266 reads in 78.405 CPU sec, 2.505 real sec
sorting block 4, #reads = 1086396 mem = 536878992 peak = 913330176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282752, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 313, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (334.67, 164.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682668 sequences (100000254 bp) in 1.560 CPU sec, 1.642 real sec...
[M::process] read 682428 sequences (100000020 bp) in 1.170 CPU sec, 1.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282810, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 316, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (339.88, 168.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684396 reads in 124.395 CPU sec, 3.977 real sec
[M::mem_process_seqs] Processed 683808 reads in 79.407 CPU sec, 2.537 real sec
sorting block 5, #reads = 1087392 mem = 536881074 peak = 913330176
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282480, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (342.55, 166.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682452 sequences (100000158 bp) in 1.872 CPU sec, 2.004 real sec...
[M::process] read 683450 sequences (100000051 bp) in 1.190 CPU sec, 1.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282443, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (340.73, 165.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682668 reads in 125.316 CPU sec, 4.001 real sec
sorting block 6, #reads = 1085633 mem = 536878329 peak = 913330176
[M::mem_process_seqs] Processed 682428 reads in 79.434 CPU sec, 2.536 real sec
sorting block 7, #reads = 1083898 mem = 536879719 peak = 916369408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282493, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (342.09, 167.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685580 sequences (100000301 bp) in 1.707 CPU sec, 1.850 real sec...
[M::process] read 684272 sequences (100000048 bp) in 1.190 CPU sec, 1.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282303, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 314, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (337.69, 166.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682452 reads in 126.805 CPU sec, 4.054 real sec
[M::mem_process_seqs] Processed 683450 reads in 80.798 CPU sec, 2.576 real sec
sorting block 8, #reads = 1084591 mem = 536878540 peak = 916369408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283426, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 307, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (328.32, 160.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683874 sequences (100000131 bp) in 1.939 CPU sec, 2.038 real sec...
[M::process] read 682196 sequences (100000122 bp) in 1.230 CPU sec, 1.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283223, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (334.52, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685580 reads in 125.112 CPU sec, 3.995 real sec
[M::mem_process_seqs] Processed 684272 reads in 78.790 CPU sec, 2.512 real sec
sorting block 9, #reads = 1085974 mem = 536880671 peak = 916369408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282565, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 318, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (339.37, 166.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681724 sequences (100000099 bp) in 1.730 CPU sec, 1.825 real sec...
[M::process] read 682012 sequences (100000250 bp) in 1.243 CPU sec, 1.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282082, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 327, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (346.47, 166.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683874 reads in 128.013 CPU sec, 4.097 real sec
sorting block 10, #reads = 1086834 mem = 536878625 peak = 916369408
[M::mem_process_seqs] Processed 682196 reads in 81.439 CPU sec, 2.598 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282318, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 327, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (346.88, 166.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 11, #reads = 1084895 mem = 536877859 peak = 916369408
[M::process] read 682070 sequences (100000253 bp) in 2.144 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282408, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (343.32, 167.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685612 sequences (100000212 bp) in 1.240 CPU sec, 1.241 real sec...
[M::mem_process_seqs] Processed 681724 reads in 128.837 CPU sec, 4.114 real sec
[M::mem_process_seqs] Processed 682012 reads in 81.946 CPU sec, 2.608 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282143, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 322, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (344.73, 168.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 12, #reads = 1084885 mem = 536877667 peak = 916369408
[M::process] read 684422 sequences (100000260 bp) in 1.890 CPU sec, 2.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283356, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 303, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (323.34, 156.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683992 sequences (100000244 bp) in 1.240 CPU sec, 1.243 real sec...
[M::mem_process_seqs] Processed 682070 reads in 128.392 CPU sec, 4.114 real sec
[M::mem_process_seqs] Processed 685612 reads in 81.503 CPU sec, 2.595 real sec
sorting block 13, #reads = 1083902 mem = 536879991 peak = 916369408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282932, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 309, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (328.68, 158.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683020 sequences (100000096 bp) in 2.118 CPU sec, 2.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282728, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (335.25, 162.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682020 sequences (100000272 bp) in 1.280 CPU sec, 1.271 real sec...
[M::mem_process_seqs] Processed 684422 reads in 126.859 CPU sec, 4.053 real sec
[M::mem_process_seqs] Processed 683992 reads in 81.314 CPU sec, 2.589 real sec
sorting block 14, #reads = 1087254 mem = 536877955 peak = 916369408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282709, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 322, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (342.75, 166.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681848 sequences (100000091 bp) in 1.925 CPU sec, 2.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282110, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (346.89, 165.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681626 sequences (100000086 bp) in 1.270 CPU sec, 1.271 real sec...
[M::mem_process_seqs] Processed 683020 reads in 129.497 CPU sec, 4.147 real sec
sorting block 15, #reads = 1085836 mem = 536881533 peak = 916369408
[M::mem_process_seqs] Processed 682020 reads in 82.989 CPU sec, 2.643 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282481, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (346.24, 166.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1085356 mem = 536879385 peak = 916369408
[M::process] read 685214 sequences (100000254 bp) in 2.110 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281802, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 322, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (344.29, 166.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686486 sequences (100000233 bp) in 1.310 CPU sec, 1.307 real sec...
[M::mem_process_seqs] Processed 681848 reads in 130.124 CPU sec, 4.154 real sec
[M::mem_process_seqs] Processed 681626 reads in 82.418 CPU sec, 2.623 real sec
sorting block 17, #reads = 1084782 mem = 536881346 peak = 916369408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283768, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 304, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (328.25, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685806 sequences (100000159 bp) in 1.984 CPU sec, 2.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284650, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 300, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (323.02, 160.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684816 sequences (100000208 bp) in 1.310 CPU sec, 1.313 real sec...
[M::mem_process_seqs] Processed 685214 reads in 133.244 CPU sec, 4.256 real sec
[M::mem_process_seqs] Processed 686486 reads in 84.979 CPU sec, 2.695 real sec
sorting block 18, #reads = 1084500 mem = 536879901 peak = 916369408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283888, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 303, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (327.18, 162.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684396 sequences (100000234 bp) in 2.296 CPU sec, 2.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283941, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 309, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (330.79, 162.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682804 sequences (100000136 bp) in 1.290 CPU sec, 1.294 real sec...
[M::mem_process_seqs] Processed 685806 reads in 133.979 CPU sec, 4.269 real sec
[M::mem_process_seqs] Processed 684816 reads in 84.970 CPU sec, 2.699 real sec
sorting block 19, #reads = 1085514 mem = 536878896 peak = 916369408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283578, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 309, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (331.03, 161.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682286 sequences (100000142 bp) in 1.900 CPU sec, 1.981 real sec...
[M::process] read 686212 sequences (100000143 bp) in 1.300 CPU sec, 1.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283406, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (335.13, 160.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684396 reads in 137.102 CPU sec, 4.395 real sec
sorting block 20, #reads = 1083055 mem = 536880540 peak = 916369408
[M::mem_process_seqs] Processed 682804 reads in 87.083 CPU sec, 2.787 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282238, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (333.46, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1082911 mem = 536877981 peak = 984727552
[M::process] read 687714 sequences (100000134 bp) in 2.235 CPU sec, 2.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284001, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 298, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (321.23, 158.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686178 sequences (100000277 bp) in 1.280 CPU sec, 1.278 real sec...
[M::mem_process_seqs] Processed 682286 reads in 134.702 CPU sec, 4.283 real sec
[M::mem_process_seqs] Processed 686212 reads in 85.882 CPU sec, 2.726 real sec
sorting block 22, #reads = 1081401 mem = 536879456 peak = 984727552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285377, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 294, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (317.38, 157.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685552 sequences (100000200 bp) in 1.942 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284697, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (320.40, 156.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684028 sequences (100000237 bp) in 1.270 CPU sec, 1.270 real sec...
[M::mem_process_seqs] Processed 687714 reads in 131.004 CPU sec, 4.171 real sec
[M::mem_process_seqs] Processed 686178 reads in 83.066 CPU sec, 2.639 real sec
sorting block 23, #reads = 1085982 mem = 536883519 peak = 984727552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284275, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 305, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (325.91, 159.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682994 sequences (100000167 bp) in 2.191 CPU sec, 2.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283630, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 311, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (331.04, 160.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682762 sequences (100000038 bp) in 1.270 CPU sec, 1.268 real sec...
[M::mem_process_seqs] Processed 685552 reads in 129.899 CPU sec, 4.128 real sec
[M::mem_process_seqs] Processed 684028 reads in 82.645 CPU sec, 2.621 real sec
sorting block 24, #reads = 1084054 mem = 536878054 peak = 984727552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283315, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (335.86, 161.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686204 sequences (100000181 bp) in 1.950 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282998, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (336.82, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686306 sequences (100000141 bp) in 1.260 CPU sec, 1.258 real sec...
[M::mem_process_seqs] Processed 682994 reads in 130.859 CPU sec, 4.167 real sec
sorting block 25, #reads = 1084508 mem = 536878139 peak = 984727552
[M::mem_process_seqs] Processed 682762 reads in 84.536 CPU sec, 2.677 real sec
sorting block 26, #reads = 1082739 mem = 536881344 peak = 1033707520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283924, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (321.59, 158.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685588 sequences (100000110 bp) in 2.250 CPU sec, 2.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284353, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 298, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (318.99, 155.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684788 sequences (100000056 bp) in 1.264 CPU sec, 1.271 real sec...
[M::mem_process_seqs] Processed 686204 reads in 133.248 CPU sec, 4.234 real sec
[M::mem_process_seqs] Processed 686306 reads in 82.076 CPU sec, 2.603 real sec
sorting block 27, #reads = 1082866 mem = 536878281 peak = 1033707520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283977, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 302, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (322.09, 155.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684044 sequences (100000217 bp) in 1.950 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283457, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (326.12, 156.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683754 sequences (100000297 bp) in 1.270 CPU sec, 1.272 real sec...
[M::mem_process_seqs] Processed 685588 reads in 128.377 CPU sec, 4.086 real sec
sorting block 28, #reads = 1085348 mem = 536881945 peak = 1033707520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283487, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 309, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (327.35, 155.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683546 sequences (100000220 bp) in 1.544 CPU sec, 1.552 real sec...
[M::mem_process_seqs] Processed 684044 reads in 77.807 CPU sec, 2.685 real sec
[M::mem_process_seqs] Processed 684788 reads in 184.205 CPU sec, 6.068 real sec
sorting block 29, #reads = 1083700 mem = 536879434 peak = 1033707520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283363, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 312, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (331.37, 158.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686462 sequences (100000138 bp) in 1.925 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283135, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 314, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (333.68, 160.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683754 reads in 144.998 CPU sec, 4.608 real sec
[M::process] read 687292 sequences (100000271 bp) in 1.360 CPU sec, 1.365 real sec...
[M::mem_process_seqs] Processed 683546 reads in 85.534 CPU sec, 2.707 real sec
sorting block 30, #reads = 1083200 mem = 536877694 peak = 1033707520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284389, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 298, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (321.47, 158.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686278 sequences (100000066 bp) in 2.216 CPU sec, 2.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284513, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 296, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (317.69, 155.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685236 sequences (100000040 bp) in 1.270 CPU sec, 1.279 real sec...
[M::mem_process_seqs] Processed 686462 reads in 135.768 CPU sec, 4.315 real sec
[M::mem_process_seqs] Processed 687292 reads in 85.058 CPU sec, 2.696 real sec
sorting block 31, #reads = 1080970 mem = 536882422 peak = 1033707520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284034, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 303, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (323.22, 156.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684512 sequences (100000039 bp) in 1.879 CPU sec, 1.980 real sec...
[M::process] read 683832 sequences (100000156 bp) in 1.280 CPU sec, 1.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284585, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 308, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (328.24, 158.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686278 reads in 132.541 CPU sec, 4.220 real sec
sorting block 32, #reads = 1085125 mem = 536880418 peak = 1033707520
[M::mem_process_seqs] Processed 685236 reads in 83.699 CPU sec, 2.656 real sec
sorting block 33, #reads = 1084513 mem = 536877259 peak = 1087479808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283642, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (328.09, 157.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682904 sequences (100000044 bp) in 2.161 CPU sec, 2.258 real sec...
[M::process] read 206854 sequences (30284880 bp) in 0.540 CPU sec, 0.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283048, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 311, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (328.93, 155.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684512 reads in 132.038 CPU sec, 4.221 real sec
[M::mem_process_seqs] Processed 683832 reads in 82.867 CPU sec, 2.651 real sec
sorting block 34, #reads = 1083322 mem = 536880000 peak = 1087479808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282738, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 313, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (331.61, 157.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 85444, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 311, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (330.85, 158.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682904 reads in 89.022 CPU sec, 2.900 real sec
[M::mem_process_seqs] Processed 206854 reads in 34.125 CPU sec, 1.108 real sec
sorting block 35, #reads = 1082352 mem = 536882863 peak = 1087479808
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 39202384
overall: 69683761152 mem 5418.729 user 313.167 sys 435.308 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.1029_0I-N1-BDNA-1_B23KGCJLT4_1.L008\tPU:L008\tSM:1029_0I-N1-BDNA-1_B23KGCJLT4_1\tLB:1029_0I-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_0I-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 1029_0I-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 435.343 sec; CPU: 5731.898 sec
sorting block 36, #reads = 305862 mem = 151954348 peak = 1087479808
uniq header count 1
merging 37 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2090680810
output reads: 39352474
bam_mem_sort: 37 calls 13.318 user 0.218 sys 13.569 real
bam_write: 37 calls 23.446 user 0.355 sys 24.123 real
execute: 1 calls 7.318 user 0.375 sys 446.252 real
merge_files: 1 calls 7.298 user 0.356 sys 10.484 real
parse_chunk: 581 calls 18.458 user 4.931 sys 23.771 real
read_chunk: 1568 calls 13.275 user 14.050 sys 135.008 real
sort_block: 1 calls 11.232 user 13.603 sys 131.360 real
write_chunk: 985 calls 6.959 user 0.242 sys 9.867 real
overall: 2357972992 mem 402.069 user 22.775 sys 446.273 real
ls: cannot access '*.cram': No such file or directory