Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f9/40c60fde9955e86a0625b33fa6d5e8/NTC_0002_0014_B23KGCJLT4_1/NTC_0002_0014_B23KGCJLT4_1_R1.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9e/ffe03d67c60fa407f1b05af83a4973/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/f9/40c60fde9955e86a0625b33fa6d5e8/NTC_0002_0014_B23KGCJLT4_1/NTC_0002_0014_B23KGCJLT4_1_R2.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9e/ffe03d67c60fa407f1b05af83a4973/.command.run ==> STAGING COMPLETE (7 inputs) cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NTC_0002_0014_B23KGCJLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 No more license available for sentieon:klib No more license available for sentieon:klib [M::bwa_idx_load_from_disk] read 3171 ALT contigs No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib [M::main_mem] bwa index loaded in 1600.453 CPU sec, 1194.863 real sec [M::process] read 1492 sequences (225292 bp) in 0.000 CPU sec, 0.002 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 1, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] skip orientation FR as there are not enough pairs [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 1492 reads in 3.051 CPU sec, 0.107 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 1492 overall: 68520288256 mem 1482.665 user 126.259 sys 1200.397 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NTC_0002_0014_B23KGCJLT4_1.L001\tPU:L001\tSM:NTC_0002_0014_B23KGCJLT4_1\tLB:NTC_0002_0014_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NTC_0002_0014_B23KGCJLT4_1_R1.fastq.gz NTC_0002_0014_B23KGCJLT4_1_R2.fastq.gz [main] Real time: 1200.434 sec; CPU: 1608.927 sec sorting block 0, #reads = 1506 mem = 840654 peak = 77185024 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 130658 output reads: 1506 bam_mem_sort: 1 calls 0.000 user 0.000 sys 0.000 real bam_write: 1 calls 0.003 user 0.000 sys 0.003 real execute: 1 calls 0.050 user 0.089 sys 1200.518 real merge_files: 1 calls 0.030 user 0.071 sys 0.109 real parse_chunk: 1 calls 0.000 user 0.000 sys 0.001 real read_chunk: 4 calls 0.001 user 0.001 sys 5.427 real sort_block: 1 calls 0.005 user 0.000 sys 5.427 real write_chunk: 1 calls 0.000 user 0.000 sys 0.000 real overall: 266199040 mem 0.079 user 0.124 sys 1200.544 real ls: cannot access '*.cram': No such file or directory