cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_rd-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1593.586 CPU sec, 1312.985 real sec
[M::process] read 685866 sequences (100000183 bp) in 0.939 CPU sec, 0.939 real sec...
[M::process] read 684662 sequences (100000060 bp) in 1.190 CPU sec, 1.193 real sec...
[M::process] read 684124 sequences (100000191 bp) in 1.220 CPU sec, 1.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283162, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 279, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (304.01, 148.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683074 sequences (100000088 bp) in 1.159 CPU sec, 1.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282819, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (309.49, 149.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282230, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (313.12, 152.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685866 reads in 204.000 CPU sec, 6.568 real sec
[M::mem_process_seqs] Processed 684662 reads in 140.426 CPU sec, 4.650 real sec
[M::mem_process_seqs] Processed 684124 reads in 153.461 CPU sec, 5.108 real sec
sorting block 0, #reads = 1087837 mem = 536881062 peak = 585330688
[M::process] read 682552 sequences (100000052 bp) in 1.841 CPU sec, 2.013 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281510, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 296, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (317.70, 153.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682706 sequences (100000065 bp) in 1.351 CPU sec, 1.378 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282192, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 300, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (321.20, 155.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683074 reads in 128.625 CPU sec, 4.112 real sec
[M::process] read 684888 sequences (100000201 bp) in 1.198 CPU sec, 1.215 real sec...
sorting block 1, #reads = 1086292 mem = 536879300 peak = 859557888
[M::mem_process_seqs] Processed 682552 reads in 80.909 CPU sec, 2.562 real sec
sorting block 2, #reads = 1086352 mem = 536880728 peak = 905183232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282405, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 298, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (318.82, 153.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683868 sequences (100000234 bp) in 1.605 CPU sec, 1.697 real sec...
[M::process] read 683024 sequences (100000223 bp) in 1.310 CPU sec, 1.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283421, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (309.37, 152.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682706 reads in 129.216 CPU sec, 4.119 real sec
[M::mem_process_seqs] Processed 684888 reads in 81.496 CPU sec, 2.597 real sec
sorting block 3, #reads = 1086148 mem = 536880488 peak = 905183232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282330, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (310.90, 151.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682896 sequences (100000116 bp) in 1.846 CPU sec, 1.935 real sec...
[M::process] read 682534 sequences (100000240 bp) in 1.270 CPU sec, 1.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282916, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (315.66, 153.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683868 reads in 129.949 CPU sec, 4.155 real sec
sorting block 4, #reads = 1088037 mem = 536881599 peak = 905183232
[M::process] read 682182 sequences (100000118 bp) in 1.336 CPU sec, 1.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281967, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (315.35, 152.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682896 reads in 76.078 CPU sec, 2.567 real sec
[M::mem_process_seqs] Processed 683024 reads in 175.121 CPU sec, 5.744 real sec
sorting block 5, #reads = 1086899 mem = 536877696 peak = 905183232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282079, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (317.37, 153.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685888 sequences (100000074 bp) in 1.963 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282218, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (319.25, 154.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685038 sequences (100000245 bp) in 1.280 CPU sec, 1.278 real sec...
[M::mem_process_seqs] Processed 682534 reads in 142.845 CPU sec, 4.556 real sec
[M::mem_process_seqs] Processed 682182 reads in 83.135 CPU sec, 2.639 real sec
sorting block 6, #reads = 1087045 mem = 536878509 peak = 905183232
[M::process] read 684694 sequences (100000226 bp) in 1.938 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283549, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 289, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (311.65, 151.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683730 sequences (100000032 bp) in 1.330 CPU sec, 1.333 real sec...
[M::mem_process_seqs] Processed 685038 reads in 71.081 CPU sec, 2.251 real sec
sorting block 7, #reads = 1087918 mem = 536885569 peak = 905183232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282801, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (306.12, 149.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282733, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 289, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (310.91, 150.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683058 sequences (100000111 bp) in 1.392 CPU sec, 1.397 real sec...
[M::mem_process_seqs] Processed 685888 reads in 203.466 CPU sec, 6.494 real sec
[M::mem_process_seqs] Processed 684694 reads in 95.482 CPU sec, 3.058 real sec
sorting block 8, #reads = 1088066 mem = 536877726 peak = 905183232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282480, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 295, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (315.85, 152.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682662 sequences (100000120 bp) in 2.247 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282246, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 301, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (321.04, 154.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685022 sequences (100000254 bp) in 1.370 CPU sec, 1.364 real sec...
[M::mem_process_seqs] Processed 683730 reads in 133.363 CPU sec, 4.260 real sec
[M::mem_process_seqs] Processed 683058 reads in 83.737 CPU sec, 2.667 real sec
sorting block 9, #reads = 1087335 mem = 536877994 peak = 905183232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282359, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 303, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (322.56, 155.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684248 sequences (100000258 bp) in 1.978 CPU sec, 2.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283046, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 286, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (310.80, 152.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682780 sequences (100000290 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_process_seqs] Processed 682662 reads in 132.696 CPU sec, 4.249 real sec
sorting block 10, #reads = 1085856 mem = 536878412 peak = 905183232
[M::mem_process_seqs] Processed 685022 reads in 83.417 CPU sec, 2.663 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282846, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 289, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (313.44, 153.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 11, #reads = 1087231 mem = 536880940 peak = 905183232
[M::process] read 682400 sequences (100000046 bp) in 2.261 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282051, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 299, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (320.24, 154.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682522 sequences (100000078 bp) in 1.400 CPU sec, 1.399 real sec...
[M::mem_process_seqs] Processed 684248 reads in 135.262 CPU sec, 4.310 real sec
[M::mem_process_seqs] Processed 682780 reads in 84.463 CPU sec, 2.685 real sec
sorting block 12, #reads = 1087421 mem = 536877676 peak = 905183232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281504, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (319.93, 154.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682638 sequences (100000055 bp) in 1.942 CPU sec, 2.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282196, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 295, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (317.53, 153.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685968 sequences (100000057 bp) in 1.430 CPU sec, 1.427 real sec...
[M::mem_process_seqs] Processed 682400 reads in 133.262 CPU sec, 4.253 real sec
[M::mem_process_seqs] Processed 682522 reads in 81.599 CPU sec, 2.584 real sec
sorting block 13, #reads = 1085656 mem = 536878767 peak = 905183232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282223, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 296, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (319.30, 155.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685888 sequences (100000252 bp) in 2.269 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283771, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (306.24, 149.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682638 reads in 126.616 CPU sec, 4.027 real sec
[M::process] read 685024 sequences (100000061 bp) in 1.516 CPU sec, 1.537 real sec...
[M::mem_process_seqs] Processed 685968 reads in 81.253 CPU sec, 2.564 real sec
sorting block 14, #reads = 1085574 mem = 536878628 peak = 905183232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283201, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (307.85, 150.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684120 sequences (100000003 bp) in 1.945 CPU sec, 2.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283320, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 287, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (310.54, 150.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685888 reads in 125.304 CPU sec, 3.993 real sec
[M::process] read 682624 sequences (100000121 bp) in 1.456 CPU sec, 1.477 real sec...
[M::mem_process_seqs] Processed 685024 reads in 79.759 CPU sec, 2.518 real sec
sorting block 15, #reads = 1088010 mem = 536879929 peak = 905183232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282510, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 294, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (315.13, 152.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1088666 mem = 536879403 peak = 923697152
[M::process] read 682284 sequences (100000094 bp) in 2.304 CPU sec, 2.470 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281966, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 303, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (322.51, 154.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684120 reads in 126.314 CPU sec, 4.016 real sec
[M::mem_process_seqs] Processed 682624 reads in 81.125 CPU sec, 2.558 real sec
[M::process] read 683878 sequences (100000031 bp) in 1.601 CPU sec, 1.623 real sec...
sorting block 17, #reads = 1086882 mem = 536885970 peak = 923697152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281884, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 305, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (323.66, 154.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683320 sequences (100000215 bp) in 1.899 CPU sec, 2.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282345, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 293, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (315.14, 152.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682284 reads in 126.773 CPU sec, 4.039 real sec
[M::process] read 682838 sequences (100000012 bp) in 1.423 CPU sec, 1.435 real sec...
[M::mem_process_seqs] Processed 683878 reads in 81.818 CPU sec, 2.586 real sec
sorting block 18, #reads = 1085807 mem = 536878411 peak = 923697152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282609, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 299, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (320.53, 155.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682338 sequences (100000099 bp) in 2.289 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282365, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 301, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (321.14, 154.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683320 reads in 125.690 CPU sec, 3.995 real sec
[M::process] read 682446 sequences (100000147 bp) in 1.502 CPU sec, 1.519 real sec...
[M::mem_process_seqs] Processed 682838 reads in 80.623 CPU sec, 2.544 real sec
sorting block 19, #reads = 1086618 mem = 536879300 peak = 923697152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282118, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 301, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (321.73, 154.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682346 sequences (100000223 bp) in 1.991 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282043, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (319.80, 154.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682338 reads in 126.757 CPU sec, 4.045 real sec
[M::process] read 685620 sequences (100000076 bp) in 1.510 CPU sec, 1.526 real sec...
[M::mem_process_seqs] Processed 682446 reads in 80.935 CPU sec, 2.560 real sec
sorting block 20, #reads = 1086648 mem = 536883565 peak = 923697152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281503, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 298, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (320.72, 155.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1086181 mem = 536877064 peak = 1011097600
[M::process] read 685606 sequences (100000005 bp) in 2.252 CPU sec, 2.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283447, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (306.00, 149.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682346 reads in 127.378 CPU sec, 4.051 real sec
[M::process] read 685196 sequences (100000253 bp) in 1.459 CPU sec, 1.467 real sec...
[M::mem_process_seqs] Processed 685620 reads in 82.239 CPU sec, 2.596 real sec
sorting block 22, #reads = 1086246 mem = 536878500 peak = 1011097600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282759, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (306.30, 148.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683946 sequences (100000296 bp) in 2.038 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282764, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 287, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (309.12, 149.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685606 reads in 125.108 CPU sec, 3.988 real sec
[M::mem_process_seqs] Processed 685196 reads in 80.985 CPU sec, 2.558 real sec
[M::process] read 682800 sequences (100000072 bp) in 1.594 CPU sec, 1.634 real sec...
sorting block 23, #reads = 1088564 mem = 536876343 peak = 1011097600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282252, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 294, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (315.40, 151.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682492 sequences (100000029 bp) in 2.216 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282223, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 300, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (319.74, 153.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683946 reads in 127.203 CPU sec, 4.047 real sec
[M::mem_process_seqs] Processed 682800 reads in 81.733 CPU sec, 2.576 real sec
[M::process] read 684108 sequences (100000180 bp) in 1.599 CPU sec, 1.626 real sec...
sorting block 24, #reads = 1087037 mem = 536876441 peak = 1011097600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282005, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 303, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (322.12, 153.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683626 sequences (100000010 bp) in 1.999 CPU sec, 2.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283228, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (311.72, 150.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682492 reads in 126.989 CPU sec, 4.058 real sec
sorting block 25, #reads = 1085238 mem = 536881525 peak = 1011097600
[M::process] read 683030 sequences (100000067 bp) in 1.630 CPU sec, 1.663 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282255, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 296, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (316.79, 152.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682712 sequences (100000062 bp) in 1.644 CPU sec, 1.648 real sec...
[M::mem_process_seqs] Processed 683626 reads in 73.792 CPU sec, 2.337 real sec
sorting block 26, #reads = 1086076 mem = 536885294 peak = 1011097600
[M::mem_process_seqs] Processed 684108 reads in 179.227 CPU sec, 5.680 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282133, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 297, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (317.82, 152.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682654 sequences (100000169 bp) in 2.022 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282208, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 298, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (319.03, 153.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683030 reads in 139.273 CPU sec, 4.422 real sec
[M::mem_process_seqs] Processed 682712 reads in 81.889 CPU sec, 2.589 real sec
sorting block 27, #reads = 1086680 mem = 536881208 peak = 1011097600
[M::process] read 682568 sequences (100000261 bp) in 1.661 CPU sec, 1.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281937, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (318.50, 153.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 28, #reads = 1085732 mem = 536877172 peak = 1011097600
[M::process] read 686710 sequences (100000207 bp) in 2.147 CPU sec, 2.256 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281925, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 296, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (318.27, 154.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682654 reads in 128.899 CPU sec, 4.099 real sec
[M::process] read 685784 sequences (100000106 bp) in 1.536 CPU sec, 1.559 real sec...
[M::mem_process_seqs] Processed 682568 reads in 82.291 CPU sec, 2.601 real sec
sorting block 29, #reads = 1085414 mem = 536877448 peak = 1011097600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283873, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (302.58, 151.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685084 sequences (100000165 bp) in 2.014 CPU sec, 2.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282932, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (306.59, 151.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686710 reads in 131.694 CPU sec, 4.199 real sec
[M::process] read 684062 sequences (100000235 bp) in 1.461 CPU sec, 1.476 real sec...
[M::mem_process_seqs] Processed 685784 reads in 83.623 CPU sec, 2.639 real sec
sorting block 30, #reads = 1085717 mem = 536878928 peak = 1011097600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283678, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 286, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (311.34, 153.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683130 sequences (100000163 bp) in 2.298 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282961, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 294, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (316.44, 154.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685084 reads in 129.164 CPU sec, 4.103 real sec
[M::process] read 683836 sequences (100000283 bp) in 1.454 CPU sec, 1.456 real sec...
[M::mem_process_seqs] Processed 684062 reads in 82.573 CPU sec, 2.609 real sec
sorting block 31, #reads = 1086826 mem = 536880932 peak = 1011097600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282606, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 296, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (318.50, 154.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685486 sequences (100000100 bp) in 2.092 CPU sec, 2.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282703, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (312.70, 150.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683130 reads in 130.251 CPU sec, 4.160 real sec
[M::mem_process_seqs] Processed 683836 reads in 83.251 CPU sec, 2.624 real sec
sorting block 32, #reads = 1085552 mem = 536880525 peak = 1011097600
[M::process] read 684930 sequences (100000213 bp) in 1.629 CPU sec, 1.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283364, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 279, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (302.23, 145.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1084913 mem = 536879429 peak = 1027739648
[M::process] read 683508 sequences (100000170 bp) in 2.202 CPU sec, 2.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283147, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (301.67, 144.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685486 reads in 130.899 CPU sec, 4.163 real sec
[M::process] read 682674 sequences (100000294 bp) in 1.527 CPU sec, 1.535 real sec...
[M::mem_process_seqs] Processed 684930 reads in 83.517 CPU sec, 2.636 real sec
sorting block 34, #reads = 1086732 mem = 536884710 peak = 1027739648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283042, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (309.04, 147.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682714 sequences (100000001 bp) in 2.041 CPU sec, 2.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282209, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (311.37, 147.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683508 reads in 133.653 CPU sec, 4.258 real sec
[M::process] read 683382 sequences (100000230 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 682674 reads in 85.885 CPU sec, 2.717 real sec
sorting block 35, #reads = 1085069 mem = 536880588 peak = 1027739648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282036, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (311.34, 147.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687306 sequences (100000237 bp) in 2.402 CPU sec, 2.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282842, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (311.81, 149.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682714 reads in 134.936 CPU sec, 4.282 real sec
[M::process] read 685816 sequences (100000284 bp) in 1.440 CPU sec, 1.438 real sec...
[M::mem_process_seqs] Processed 683382 reads in 86.000 CPU sec, 2.717 real sec
sorting block 36, #reads = 1083530 mem = 536884449 peak = 1027739648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284246, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 272, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (299.78, 148.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684568 sequences (100000006 bp) in 2.044 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283718, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (304.16, 147.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687306 reads in 131.870 CPU sec, 4.200 real sec
[M::process] read 683604 sequences (100000242 bp) in 1.445 CPU sec, 1.449 real sec...
sorting block 37, #reads = 1084198 mem = 536878072 peak = 1027739648
[M::mem_process_seqs] Processed 685816 reads in 84.116 CPU sec, 2.658 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283396, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 286, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (308.74, 148.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1086646 mem = 536882822 peak = 1090789376
[M::process] read 683250 sequences (100000268 bp) in 2.418 CPU sec, 2.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282570, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 292, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (313.66, 150.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684568 reads in 129.696 CPU sec, 4.122 real sec
[M::mem_process_seqs] Processed 683604 reads in 82.942 CPU sec, 2.617 real sec
[M::process] read 684824 sequences (100000027 bp) in 1.633 CPU sec, 1.660 real sec...
sorting block 39, #reads = 1086162 mem = 536881500 peak = 1090789376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282816, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 296, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (316.99, 152.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685144 sequences (100000239 bp) in 1.982 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282955, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (307.28, 147.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683250 reads in 131.462 CPU sec, 4.186 real sec
[M::process] read 683718 sequences (100000270 bp) in 1.454 CPU sec, 1.459 real sec...
[M::mem_process_seqs] Processed 684824 reads in 84.880 CPU sec, 2.681 real sec
sorting block 40, #reads = 1084578 mem = 536881133 peak = 1090789376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283815, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 281, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (304.22, 146.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683192 sequences (100000075 bp) in 2.423 CPU sec, 2.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282934, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (313.38, 149.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685144 reads in 129.446 CPU sec, 4.113 real sec
[M::mem_process_seqs] Processed 683718 reads in 82.394 CPU sec, 2.601 real sec
[M::process] read 682518 sequences (100000268 bp) in 1.573 CPU sec, 1.592 real sec...
sorting block 41, #reads = 1084447 mem = 536878156 peak = 1090789376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282608, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (313.72, 149.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1085601 mem = 536878006 peak = 1090789376
[M::process] read 682274 sequences (100000153 bp) in 2.067 CPU sec, 2.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282207, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 297, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (317.18, 151.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683192 reads in 129.381 CPU sec, 4.124 real sec
[M::mem_process_seqs] Processed 682518 reads in 83.435 CPU sec, 2.632 real sec
[M::process] read 686110 sequences (100000214 bp) in 1.687 CPU sec, 1.720 real sec...
sorting block 43, #reads = 1084671 mem = 536878310 peak = 1090789376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281688, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (317.08, 151.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686126 sequences (100000045 bp) in 2.272 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283628, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 276, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (302.23, 148.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 1, 704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2110)
[M::mem_pestat] mean and std.dev: (212.00, 322.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2813)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 685574 sequences (100000253 bp) in 1.380 CPU sec, 1.382 real sec...
[M::mem_process_seqs] Processed 682274 reads in 138.320 CPU sec, 4.396 real sec
[M::mem_process_seqs] Processed 686110 reads in 90.792 CPU sec, 2.876 real sec
sorting block 44, #reads = 1081886 mem = 536878495 peak = 1090789376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283734, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 279, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (303.53, 148.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684432 sequences (100000289 bp) in 2.062 CPU sec, 2.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283549, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 282, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (305.85, 148.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686126 reads in 130.732 CPU sec, 4.157 real sec
[M::process] read 683320 sequences (100000067 bp) in 1.430 CPU sec, 1.431 real sec...
[M::mem_process_seqs] Processed 685574 reads in 83.770 CPU sec, 2.648 real sec
sorting block 45, #reads = 1084379 mem = 536878390 peak = 1090789376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282924, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (310.19, 148.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683386 sequences (100000188 bp) in 2.561 CPU sec, 2.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282752, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 295, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (314.99, 150.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684432 reads in 129.264 CPU sec, 4.107 real sec
[M::mem_process_seqs] Processed 683320 reads in 82.971 CPU sec, 2.614 real sec
sorting block 46, #reads = 1086644 mem = 536879071 peak = 1090789376
[M::process] read 684616 sequences (100000274 bp) in 1.774 CPU sec, 1.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282711, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (313.14, 148.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1086652 mem = 536880633 peak = 1090789376
[M::process] read 684332 sequences (100000252 bp) in 2.039 CPU sec, 2.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283627, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 286, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (306.84, 146.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683386 reads in 132.669 CPU sec, 4.237 real sec
[M::mem_process_seqs] Processed 684616 reads in 82.683 CPU sec, 2.609 real sec
[M::process] read 683696 sequences (100000166 bp) in 1.683 CPU sec, 1.734 real sec...
sorting block 48, #reads = 1083920 mem = 536880612 peak = 1090789376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282896, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (308.98, 146.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683568 sequences (100000242 bp) in 2.238 CPU sec, 2.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282753, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (311.45, 148.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684332 reads in 131.151 CPU sec, 4.172 real sec
[M::mem_process_seqs] Processed 683696 reads in 84.184 CPU sec, 2.655 real sec
[M::process] read 683742 sequences (100000269 bp) in 1.567 CPU sec, 1.585 real sec...
sorting block 49, #reads = 1085948 mem = 536878471 peak = 1090789376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283045, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (311.29, 147.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684176 sequences (100000207 bp) in 2.004 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282592, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (310.44, 147.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688894 sequences (100000113 bp) in 1.400 CPU sec, 1.403 real sec...
[M::mem_process_seqs] Processed 683568 reads in 132.919 CPU sec, 4.230 real sec
[M::mem_process_seqs] Processed 683742 reads in 84.907 CPU sec, 2.688 real sec
sorting block 50, #reads = 1084898 mem = 536878035 peak = 1090789376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282330, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (312.81, 152.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686814 sequences (100000145 bp) in 2.292 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284319, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 262, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (292.79, 146.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685410 sequences (100000069 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_process_seqs] Processed 684176 reads in 141.535 CPU sec, 4.496 real sec
sorting block 51, #reads = 1083747 mem = 536880715 peak = 1090789376
[M::mem_process_seqs] Processed 688894 reads in 86.686 CPU sec, 2.748 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283917, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 275, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (302.82, 150.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 52, #reads = 1081619 mem = 536892537 peak = 1098768384
[M::process] read 684406 sequences (100000296 bp) in 1.971 CPU sec, 2.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283708, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (308.76, 151.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686814 reads in 132.067 CPU sec, 4.206 real sec
[M::process] read 683482 sequences (100000093 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 685410 reads in 83.815 CPU sec, 2.651 real sec
sorting block 53, #reads = 1086585 mem = 536878442 peak = 1098768384
[M::process] read 685062 sequences (100000174 bp) in 2.356 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283017, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 294, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (316.45, 153.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683482 reads in 72.755 CPU sec, 2.298 real sec
[M::process] read 685038 sequences (100000027 bp) in 1.483 CPU sec, 1.489 real sec...
sorting block 54, #reads = 1085933 mem = 536879523 peak = 1098768384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283092, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 291, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (313.72, 152.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284055, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (305.81, 145.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684406 reads in 205.274 CPU sec, 6.520 real sec
[M::process] read 684290 sequences (100000270 bp) in 1.476 CPU sec, 1.485 real sec...
[M::mem_process_seqs] Processed 685062 reads in 99.340 CPU sec, 3.157 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283275, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 282, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (303.74, 144.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1084394 mem = 536879475 peak = 1098768384
[M::process] read 683984 sequences (100000026 bp) in 2.274 CPU sec, 2.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282897, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (308.66, 147.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685038 reads in 132.070 CPU sec, 4.209 real sec
[M::mem_process_seqs] Processed 684290 reads in 84.497 CPU sec, 2.667 real sec
[M::process] read 683494 sequences (100000070 bp) in 1.572 CPU sec, 1.610 real sec...
sorting block 56, #reads = 1085264 mem = 536877798 peak = 1098768384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283061, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (309.67, 147.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683094 sequences (100000269 bp) in 2.179 CPU sec, 2.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282410, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (311.45, 147.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 429686 sequences (62930124 bp) in 1.000 CPU sec, 1.008 real sec...
[M::mem_process_seqs] Processed 683984 reads in 129.179 CPU sec, 4.115 real sec
[M::mem_process_seqs] Processed 683494 reads in 81.968 CPU sec, 2.602 real sec
sorting block 57, #reads = 1086332 mem = 536876353 peak = 1098768384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283020, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 293, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (313.17, 148.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 177457, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (316.51, 152.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683094 reads in 105.697 CPU sec, 3.439 real sec
[M::mem_process_seqs] Processed 429686 reads in 55.733 CPU sec, 1.810 real sec
sorting block 58, #reads = 1085117 mem = 536880439 peak = 1181483008
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 64728734
overall: 69850886144 mem 8074.991 user 292.442 sys 1537.825 real
sorting block 59, #reads = 963249 mem = 476743697 peak = 1200107520
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.659_rd-N1-BDNA-1_B23KGCJLT4_1.L004\tPU:L004\tSM:659_rd-N1-BDNA-1_B23KGCJLT4_1\tLB:659_rd-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_rd-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 659_rd-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 1537.879 sec; CPU: 8367.449 sec
uniq header count 1
merging 60 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3332930553
output reads: 65034675
bam_mem_sort: 60 calls 23.020 user 0.326 sys 23.447 real
bam_write: 60 calls 46.808 user 0.874 sys 48.018 real
execute: 1 calls 13.246 user 0.486 sys 1556.129 real
merge_files: 1 calls 13.229 user 0.450 sys 17.067 real
parse_chunk: 959 calls 28.763 user 8.758 sys 38.229 real
read_chunk: 2585 calls 23.357 user 23.888 sys 224.447 real
sort_block: 1 calls 19.150 user 23.427 sys 218.536 real
write_chunk: 1624 calls 12.825 user 0.324 sys 16.317 real
overall: 2713591808 mem 663.088 user 39.599 sys 1556.153 real
ls: cannot access '*.cram': No such file or directory