.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_Lx-N1-BDNA-1_B23KGCJLT4_1;L002;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c6/0f74e904de5c2842602883c9c61bd4/.command.err
Size: 56.7 KB
Task: DAQ:SENTIEON_BWAMEM (659_Lx-N1-BDNA-1_B23KGCJLT4_1;L002;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_Lx-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1722.756 CPU sec, 966.389 real sec
[M::process] read 679726 sequences (100000217 bp) in 0.955 CPU sec, 0.956 real sec...
[M::process] read 678084 sequences (100000033 bp) in 1.221 CPU sec, 1.246 real sec...
[M::process] read 677690 sequences (100000291 bp) in 1.245 CPU sec, 1.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281637, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (347.63, 163.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678486 sequences (100000038 bp) in 1.187 CPU sec, 1.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281405, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 339, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (353.95, 163.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281645, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 345, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (360.04, 167.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679726 reads in 215.569 CPU sec, 6.960 real sec
[M::mem_process_seqs] Processed 678084 reads in 143.644 CPU sec, 4.669 real sec
[M::mem_process_seqs] Processed 677690 reads in 155.681 CPU sec, 5.075 real sec
sorting block 0, #reads = 1083026 mem = 536883601 peak = 605429760
[M::process] read 678788 sequences (100000086 bp) in 1.782 CPU sec, 1.932 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281726, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (352.63, 165.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679470 sequences (100000018 bp) in 1.292 CPU sec, 1.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280833, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (347.64, 163.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679150 sequences (100000267 bp) in 1.149 CPU sec, 1.155 real sec...
[M::mem_process_seqs] Processed 678486 reads in 136.099 CPU sec, 4.336 real sec
sorting block 1, #reads = 1081986 mem = 536883188 peak = 835067904
[M::mem_process_seqs] Processed 678788 reads in 85.932 CPU sec, 2.728 real sec
sorting block 2, #reads = 1082348 mem = 536881683 peak = 877137920
[M::process] read 678758 sequences (100000004 bp) in 1.597 CPU sec, 1.695 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281485, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (345.42, 165.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678978 sequences (100000215 bp) in 1.253 CPU sec, 1.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281733, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (347.87, 166.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679470 reads in 135.671 CPU sec, 4.330 real sec
[M::mem_process_seqs] Processed 679150 reads in 84.438 CPU sec, 2.700 real sec
sorting block 3, #reads = 1082802 mem = 536879145 peak = 877137920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281543, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (351.84, 168.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678510 sequences (100000114 bp) in 1.816 CPU sec, 1.944 real sec...
[M::process] read 677876 sequences (100000076 bp) in 1.230 CPU sec, 1.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281557, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (353.18, 169.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678758 reads in 134.847 CPU sec, 4.306 real sec
[M::mem_process_seqs] Processed 678978 reads in 84.564 CPU sec, 2.697 real sec
sorting block 4, #reads = 1083065 mem = 536878094 peak = 877137920
[M::process] read 677554 sequences (100000155 bp) in 1.824 CPU sec, 1.971 real sec...
[M::process] read 678404 sequences (100000296 bp) in 1.277 CPU sec, 1.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281280, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (362.23, 167.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677876 reads in 76.854 CPU sec, 2.439 real sec
sorting block 5, #reads = 1082458 mem = 536877950 peak = 877137920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281213, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (353.59, 163.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281704, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (359.56, 165.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678768 sequences (100000241 bp) in 1.417 CPU sec, 1.419 real sec...
[M::mem_process_seqs] Processed 678510 reads in 205.635 CPU sec, 6.578 real sec
[M::mem_process_seqs] Processed 677554 reads in 97.295 CPU sec, 3.129 real sec
sorting block 6, #reads = 1083773 mem = 536879816 peak = 877137920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281256, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (353.44, 164.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679072 sequences (100000109 bp) in 2.365 CPU sec, 2.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281437, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (350.76, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679040 sequences (100000268 bp) in 1.320 CPU sec, 1.315 real sec...
[M::mem_process_seqs] Processed 678404 reads in 136.451 CPU sec, 4.350 real sec
[M::mem_process_seqs] Processed 678768 reads in 86.668 CPU sec, 2.754 real sec
sorting block 7, #reads = 1081856 mem = 536881656 peak = 877137920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281477, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (348.86, 166.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678826 sequences (100000283 bp) in 1.880 CPU sec, 2.024 real sec...
[M::process] read 679166 sequences (100000287 bp) in 1.370 CPU sec, 1.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281659, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (351.27, 168.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679072 reads in 137.967 CPU sec, 4.405 real sec
sorting block 8, #reads = 1082572 mem = 536878697 peak = 877137920
[M::mem_process_seqs] Processed 679040 reads in 86.347 CPU sec, 2.745 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281928, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 335, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (355.16, 170.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1082855 mem = 536879016 peak = 877137920
[M::process] read 678168 sequences (100000121 bp) in 2.202 CPU sec, 2.357 real sec...
[M::process] read 677890 sequences (100000226 bp) in 1.350 CPU sec, 1.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281736, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (352.40, 166.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678826 reads in 137.936 CPU sec, 4.399 real sec
[M::mem_process_seqs] Processed 679166 reads in 86.718 CPU sec, 2.758 real sec
sorting block 10, #reads = 1082564 mem = 536878517 peak = 877137920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281675, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (359.79, 167.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678938 sequences (100000023 bp) in 2.268 CPU sec, 2.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281487, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 346, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (359.62, 166.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678310 sequences (100000263 bp) in 1.370 CPU sec, 1.373 real sec...
[M::mem_process_seqs] Processed 678168 reads in 136.073 CPU sec, 4.346 real sec
[M::mem_process_seqs] Processed 677890 reads in 85.901 CPU sec, 2.736 real sec
sorting block 11, #reads = 1082016 mem = 536879596 peak = 877137920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282122, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (353.94, 166.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678726 sequences (100000093 bp) in 2.175 CPU sec, 2.339 real sec...
[M::process] read 678792 sequences (100000088 bp) in 1.360 CPU sec, 1.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281516, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (355.33, 166.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678938 reads in 136.654 CPU sec, 4.367 real sec
[M::mem_process_seqs] Processed 678310 reads in 86.659 CPU sec, 2.763 real sec
sorting block 12, #reads = 1082021 mem = 536879909 peak = 877137920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282097, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (351.90, 167.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678698 sequences (100000076 bp) in 2.281 CPU sec, 2.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281742, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (352.51, 168.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679344 sequences (100000079 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_process_seqs] Processed 678726 reads in 138.372 CPU sec, 4.410 real sec
sorting block 13, #reads = 1082404 mem = 536879918 peak = 877137920
[M::mem_process_seqs] Processed 678792 reads in 87.228 CPU sec, 2.771 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281877, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (353.97, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1082957 mem = 536879695 peak = 911007744
[M::process] read 678746 sequences (100000238 bp) in 2.253 CPU sec, 2.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281583, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 333, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (353.99, 171.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677900 sequences (100000251 bp) in 1.370 CPU sec, 1.362 real sec...
[M::mem_process_seqs] Processed 678698 reads in 138.841 CPU sec, 4.426 real sec
[M::mem_process_seqs] Processed 679344 reads in 87.656 CPU sec, 2.784 real sec
sorting block 15, #reads = 1082542 mem = 536879743 peak = 911007744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281473, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 338, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (354.87, 167.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678296 sequences (100000026 bp) in 2.324 CPU sec, 2.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281402, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (355.02, 164.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678118 sequences (100000114 bp) in 1.380 CPU sec, 1.374 real sec...
[M::mem_process_seqs] Processed 678746 reads in 134.391 CPU sec, 4.283 real sec
[M::mem_process_seqs] Processed 677900 reads in 83.850 CPU sec, 2.662 real sec
sorting block 16, #reads = 1084211 mem = 536881234 peak = 911007744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281291, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (353.33, 165.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678548 sequences (100000046 bp) in 2.498 CPU sec, 2.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280871, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (353.17, 165.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678296 reads in 131.470 CPU sec, 4.188 real sec
[M::mem_process_seqs] Processed 678118 reads in 84.947 CPU sec, 2.679 real sec
[M::process] read 678802 sequences (100000127 bp) in 1.687 CPU sec, 1.749 real sec...
sorting block 17, #reads = 1082815 mem = 536879493 peak = 911007744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281081, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (350.04, 164.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678674 sequences (100000162 bp) in 2.145 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281510, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (349.65, 166.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678548 reads in 132.966 CPU sec, 4.226 real sec
[M::process] read 679216 sequences (100000025 bp) in 1.592 CPU sec, 1.613 real sec...
sorting block 18, #reads = 1082667 mem = 536878839 peak = 911007744
[M::mem_process_seqs] Processed 678802 reads in 85.583 CPU sec, 2.699 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281455, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (352.10, 167.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1082142 mem = 536880782 peak = 955813888
[M::process] read 679854 sequences (100000027 bp) in 2.625 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282036, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 331, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (351.79, 169.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678674 reads in 131.967 CPU sec, 4.200 real sec
[M::mem_process_seqs] Processed 679216 reads in 84.709 CPU sec, 2.676 real sec
[M::process] read 678660 sequences (100000227 bp) in 1.668 CPU sec, 1.737 real sec...
sorting block 20, #reads = 1082309 mem = 536878029 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282038, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 330, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (349.18, 167.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678762 sequences (100000041 bp) in 2.177 CPU sec, 2.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281406, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (351.61, 163.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679854 reads in 130.715 CPU sec, 4.159 real sec
[M::process] read 679238 sequences (100000097 bp) in 1.537 CPU sec, 1.560 real sec...
[M::mem_process_seqs] Processed 678660 reads in 83.425 CPU sec, 2.633 real sec
sorting block 21, #reads = 1083216 mem = 536878205 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281911, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (351.53, 163.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679442 sequences (100000165 bp) in 2.751 CPU sec, 2.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281939, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 324, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (341.35, 159.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678762 reads in 131.043 CPU sec, 4.180 real sec
[M::mem_process_seqs] Processed 679238 reads in 84.078 CPU sec, 2.654 real sec
sorting block 22, #reads = 1083084 mem = 536877494 peak = 955813888
[M::process] read 679112 sequences (100000171 bp) in 2.417 CPU sec, 2.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281992, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 326, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (342.63, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679100 sequences (100000146 bp) in 1.975 CPU sec, 2.111 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281867, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 323, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (340.63, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679442 reads in 134.144 CPU sec, 4.292 real sec
[M::mem_process_seqs] Processed 679112 reads in 85.095 CPU sec, 2.691 real sec
sorting block 23, #reads = 1082439 mem = 536881133 peak = 955813888
[M::process] read 679050 sequences (100000131 bp) in 2.294 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281207, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 320, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (337.87, 156.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1082054 mem = 536878112 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281441, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 320, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (339.37, 158.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679876 sequences (100000177 bp) in 2.609 CPU sec, 2.827 real sec...
[M::mem_process_seqs] Processed 679100 reads in 137.484 CPU sec, 4.407 real sec
[M::mem_process_seqs] Processed 679050 reads in 88.345 CPU sec, 2.808 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281562, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (343.06, 163.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678778 sequences (100000221 bp) in 2.552 CPU sec, 2.759 real sec...
[M::mem_process_seqs] Processed 679876 reads in 75.287 CPU sec, 2.497 real sec
sorting block 25, #reads = 1080934 mem = 536877493 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282099, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (347.28, 160.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678778 reads in 71.435 CPU sec, 2.365 real sec
[M::process] read 678176 sequences (100000118 bp) in 2.622 CPU sec, 2.834 real sec...
sorting block 26, #reads = 1081817 mem = 536881845 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281876, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (347.77, 158.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678176 reads in 71.910 CPU sec, 2.384 real sec
[M::process] read 679090 sequences (100000051 bp) in 2.723 CPU sec, 2.936 real sec...
sorting block 27, #reads = 1082563 mem = 536880691 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281636, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 324, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (340.41, 157.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679090 reads in 75.563 CPU sec, 2.471 real sec
[M::process] read 679208 sequences (100000083 bp) in 2.558 CPU sec, 2.778 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282129, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 325, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (341.03, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678908 sequences (100000020 bp) in 2.192 CPU sec, 2.366 real sec...
[M::mem_process_seqs] Processed 679208 reads in 76.226 CPU sec, 2.461 real sec
sorting block 28, #reads = 1081791 mem = 536878077 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282025, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 325, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (342.01, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678908 reads in 73.926 CPU sec, 2.391 real sec
[M::process] read 678908 sequences (100000185 bp) in 2.494 CPU sec, 2.722 real sec...
sorting block 29, #reads = 1082171 mem = 536878723 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281602, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (344.77, 161.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678552 sequences (100000012 bp) in 2.213 CPU sec, 2.379 real sec...
[M::mem_process_seqs] Processed 678908 reads in 75.149 CPU sec, 2.431 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280992, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (346.87, 163.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678552 reads in 76.876 CPU sec, 2.484 real sec
[M::process] read 679640 sequences (100000056 bp) in 2.350 CPU sec, 2.555 real sec...
sorting block 30, #reads = 1082174 mem = 536879780 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281423, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (344.45, 164.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678994 sequences (100000141 bp) in 2.167 CPU sec, 2.329 real sec...
[M::mem_process_seqs] Processed 679640 reads in 83.535 CPU sec, 2.692 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281400, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (346.63, 161.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678516 sequences (100000041 bp) in 2.262 CPU sec, 2.450 real sec...
sorting block 31, #reads = 1078525 mem = 536879529 peak = 955813888
[M::mem_process_seqs] Processed 678994 reads in 76.783 CPU sec, 2.485 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281722, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.93, 160.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678516 reads in 77.441 CPU sec, 2.507 real sec
[M::process] read 679578 sequences (100000221 bp) in 2.877 CPU sec, 3.040 real sec...
sorting block 32, #reads = 1082167 mem = 536880393 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282617, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 322, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (340.20, 159.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679562 sequences (100000074 bp) in 2.670 CPU sec, 2.844 real sec...
[M::mem_process_seqs] Processed 679578 reads in 93.619 CPU sec, 3.018 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282409, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 322, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (339.59, 158.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679510 sequences (100000054 bp) in 2.690 CPU sec, 2.877 real sec...
[M::mem_process_seqs] Processed 679562 reads in 100.273 CPU sec, 3.210 real sec
sorting block 33, #reads = 1077726 mem = 536878043 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281951, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (340.61, 159.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678708 sequences (100000021 bp) in 2.709 CPU sec, 2.902 real sec...
[M::mem_process_seqs] Processed 679510 reads in 86.626 CPU sec, 2.780 real sec
sorting block 34, #reads = 1074971 mem = 536882913 peak = 955813888
[M::process] read 678716 sequences (100000074 bp) in 2.099 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281821, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (345.27, 160.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680416 sequences (100000083 bp) in 1.480 CPU sec, 1.476 real sec...
[M::process] read 679058 sequences (100000015 bp) in 1.170 CPU sec, 1.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281692, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (348.34, 164.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678708 reads in 159.870 CPU sec, 5.045 real sec
[M::mem_process_seqs] Processed 678716 reads in 110.132 CPU sec, 3.486 real sec
sorting block 35, #reads = 1076626 mem = 536876783 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282238, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 322, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (342.75, 164.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679148 sequences (100000024 bp) in 2.769 CPU sec, 2.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281837, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (347.32, 162.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680416 reads in 144.305 CPU sec, 4.568 real sec
[M::mem_process_seqs] Processed 679058 reads in 89.835 CPU sec, 2.829 real sec
[M::process] read 679022 sequences (100000294 bp) in 2.040 CPU sec, 2.104 real sec...
sorting block 36, #reads = 1075739 mem = 536877504 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281420, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 325, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (341.38, 157.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678706 sequences (100000034 bp) in 2.186 CPU sec, 2.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281192, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 327, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (343.06, 159.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (316, 477, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (395.83, 124.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RR
[M::process] read 678394 sequences (100000155 bp) in 1.510 CPU sec, 1.504 real sec...
[M::mem_process_seqs] Processed 679148 reads in 149.435 CPU sec, 4.747 real sec
[M::mem_process_seqs] Processed 679022 reads in 96.347 CPU sec, 3.050 real sec
sorting block 37, #reads = 1082990 mem = 536878619 peak = 955813888
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281473, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (348.31, 162.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1080124 mem = 536878383 peak = 976465920
[M::process] read 678102 sequences (100000273 bp) in 2.246 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281102, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (345.78, 159.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677944 sequences (100000117 bp) in 1.610 CPU sec, 1.610 real sec...
[M::mem_process_seqs] Processed 678706 reads in 145.343 CPU sec, 4.614 real sec
[M::mem_process_seqs] Processed 678394 reads in 93.165 CPU sec, 2.943 real sec
[M::process] read 197132 sequences (29076825 bp) in 1.113 CPU sec, 1.208 real sec...
sorting block 39, #reads = 1081668 mem = 536880321 peak = 976465920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281484, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (345.45, 158.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280786, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (352.49, 164.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678102 reads in 139.757 CPU sec, 4.514 real sec
[M::mem_process_seqs] Processed 677944 reads in 88.330 CPU sec, 2.861 real sec
sorting block 40, #reads = 1081514 mem = 536880217 peak = 976465920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 81795, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (353.78, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 197132 reads in 23.964 CPU sec, 0.810 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 44999372
overall: 69726957568 mem 6421.983 user 374.237 sys 1138.064 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.659_Lx-N1-BDNA-1_B23KGCJLT4_1.L002\tPU:L002\tSM:659_Lx-N1-BDNA-1_B23KGCJLT4_1\tLB:659_Lx-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_Lx-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 659_Lx-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 1138.104 sec; CPU: 6796.229 sec
sorting block 41, #reads = 810444 mem = 402776534 peak = 987947008
uniq header count 1
merging 42 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2273131642
output reads: 45160126
bam_mem_sort: 42 calls 18.951 user 0.303 sys 19.339 real
bam_write: 42 calls 8.402 user 0.285 sys 8.674 real
execute: 1 calls 10.734 user 0.361 sys 1151.053 real
merge_files: 1 calls 10.714 user 0.342 sys 12.461 real
parse_chunk: 669 calls 26.251 user 6.391 sys 33.028 real
read_chunk: 1803 calls 14.523 user 15.934 sys 168.595 real
sort_block: 1 calls 12.541 user 15.441 sys 164.264 real
write_chunk: 1132 calls 10.408 user 0.191 sys 11.839 real
overall: 2426572800 mem 485.112 user 26.537 sys 1151.076 real
ls: cannot access '*.cram': No such file or directory