.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_Fr-N1-BDNA-1_B23KGCJLT4_1;L007;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5b/11ab4d2bad86921c894c99ad04e21b/.command.err
Size: 63.8 KB
Task: DAQ:SENTIEON_BWAMEM (659_Fr-N1-BDNA-1_B23KGCJLT4_1;L007;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_Fr-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1582.589 CPU sec, 772.573 real sec
[M::process] read 681596 sequences (100000260 bp) in 0.907 CPU sec, 0.916 real sec...
[M::process] read 680678 sequences (100000149 bp) in 1.168 CPU sec, 1.177 real sec...
[M::process] read 680088 sequences (100000032 bp) in 1.124 CPU sec, 1.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282736, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 285, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (306.50, 146.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679680 sequences (100000115 bp) in 1.100 CPU sec, 1.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282462, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 292, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (312.67, 149.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281861, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 298, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (316.95, 151.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681596 reads in 194.636 CPU sec, 6.267 real sec
[M::mem_process_seqs] Processed 680678 reads in 132.160 CPU sec, 4.293 real sec
sorting block 0, #reads = 1085118 mem = 536877935 peak = 595128320
[M::process] read 679064 sequences (100000281 bp) in 1.375 CPU sec, 1.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281829, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 301, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (319.62, 152.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679680 reads in 66.612 CPU sec, 2.104 real sec
[M::process] read 678788 sequences (100000156 bp) in 1.302 CPU sec, 1.353 real sec...
[M::mem_process_seqs] Processed 680088 reads in 233.408 CPU sec, 7.520 real sec
sorting block 1, #reads = 1083976 mem = 536880551 peak = 727076864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282292, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 308, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (326.01, 156.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678152 sequences (100000042 bp) in 1.581 CPU sec, 1.667 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281332, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 310, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (328.58, 158.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677860 sequences (100000224 bp) in 1.110 CPU sec, 1.110 real sec...
[M::mem_process_seqs] Processed 679064 reads in 131.845 CPU sec, 4.170 real sec
[M::mem_process_seqs] Processed 678788 reads in 76.732 CPU sec, 2.444 real sec
sorting block 2, #reads = 1084062 mem = 536880951 peak = 840785920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281479, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 314, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (332.17, 159.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677740 sequences (100000137 bp) in 1.749 CPU sec, 1.877 real sec...
[M::process] read 678926 sequences (100000007 bp) in 1.170 CPU sec, 1.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281319, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 314, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (331.56, 157.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678152 reads in 123.476 CPU sec, 3.935 real sec
sorting block 3, #reads = 1082229 mem = 536880649 peak = 840785920
[M::mem_process_seqs] Processed 677860 reads in 77.649 CPU sec, 2.476 real sec
sorting block 4, #reads = 1082870 mem = 536877920 peak = 888479744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281089, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 313, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (331.32, 158.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680656 sequences (100000028 bp) in 1.733 CPU sec, 1.844 real sec...
[M::process] read 680300 sequences (100000069 bp) in 1.160 CPU sec, 1.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281539, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 304, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (324.14, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677740 reads in 125.542 CPU sec, 4.006 real sec
[M::mem_process_seqs] Processed 678926 reads in 78.799 CPU sec, 2.517 real sec
sorting block 5, #reads = 1082407 mem = 536881485 peak = 888479744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282075, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 296, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (315.54, 151.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679522 sequences (100000143 bp) in 1.843 CPU sec, 1.973 real sec...
[M::process] read 679060 sequences (100000038 bp) in 1.200 CPU sec, 1.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282294, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 299, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (317.70, 152.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680656 reads in 123.724 CPU sec, 3.952 real sec
[M::mem_process_seqs] Processed 680300 reads in 77.735 CPU sec, 2.483 real sec
sorting block 6, #reads = 1084569 mem = 536879316 peak = 888479744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282196, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 304, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (322.66, 154.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678696 sequences (100000214 bp) in 1.804 CPU sec, 1.901 real sec...
[M::process] read 678502 sequences (100000156 bp) in 1.250 CPU sec, 1.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281491, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 308, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (325.35, 155.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679522 reads in 124.888 CPU sec, 3.989 real sec
[M::mem_process_seqs] Processed 679060 reads in 78.286 CPU sec, 2.505 real sec
sorting block 7, #reads = 1083860 mem = 536879928 peak = 888500224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281825, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 311, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (329.56, 158.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678116 sequences (100000089 bp) in 1.946 CPU sec, 2.052 real sec...
[M::process] read 678230 sequences (100000199 bp) in 1.280 CPU sec, 1.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281287, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 313, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (330.12, 158.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678696 reads in 127.218 CPU sec, 4.062 real sec
sorting block 8, #reads = 1084085 mem = 536878396 peak = 888500224
[M::mem_process_seqs] Processed 678502 reads in 79.279 CPU sec, 2.525 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281309, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 312, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (330.23, 157.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1084647 mem = 536884656 peak = 906190848
[M::process] read 678436 sequences (100000084 bp) in 1.940 CPU sec, 2.037 real sec...
[M::process] read 680114 sequences (100000056 bp) in 1.280 CPU sec, 1.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281850, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 309, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (329.03, 158.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678116 reads in 127.730 CPU sec, 4.079 real sec
[M::mem_process_seqs] Processed 678230 reads in 80.316 CPU sec, 2.560 real sec
sorting block 10, #reads = 1082720 mem = 536878125 peak = 906190848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281619, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 308, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (329.50, 160.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680264 sequences (100000284 bp) in 2.095 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281722, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 296, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (316.80, 152.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679576 sequences (100000233 bp) in 1.290 CPU sec, 1.287 real sec...
[M::mem_process_seqs] Processed 678436 reads in 128.253 CPU sec, 4.093 real sec
[M::mem_process_seqs] Processed 680114 reads in 80.310 CPU sec, 2.556 real sec
sorting block 11, #reads = 1083005 mem = 536876725 peak = 906190848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282107, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 298, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (317.39, 152.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679056 sequences (100000100 bp) in 1.794 CPU sec, 1.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282309, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 678488 sequences (100000109 bp) in 1.310 CPU sec, 1.304 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (192, 302, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (320.86, 153.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680264 reads in 127.484 CPU sec, 4.088 real sec
[M::mem_process_seqs] Processed 679576 reads in 79.606 CPU sec, 2.541 real sec
sorting block 12, #reads = 1083751 mem = 536877318 peak = 906190848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282018, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 306, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (323.63, 154.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678084 sequences (100000124 bp) in 2.024 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281713, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 311, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (328.85, 156.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677860 sequences (100000239 bp) in 1.310 CPU sec, 1.303 real sec...
[M::mem_process_seqs] Processed 679056 reads in 126.705 CPU sec, 4.050 real sec
sorting block 13, #reads = 1083830 mem = 536880195 peak = 906190848
[M::mem_process_seqs] Processed 678488 reads in 79.980 CPU sec, 2.544 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281215, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 315, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (331.61, 158.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1083526 mem = 536876267 peak = 939679744
[M::process] read 678114 sequences (100000247 bp) in 2.132 CPU sec, 2.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280933, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 315, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (332.68, 159.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678226 sequences (100000014 bp) in 1.300 CPU sec, 1.304 real sec...
[M::mem_process_seqs] Processed 678084 reads in 130.687 CPU sec, 4.177 real sec
[M::mem_process_seqs] Processed 677860 reads in 82.027 CPU sec, 2.614 real sec
sorting block 15, #reads = 1083480 mem = 536880394 peak = 939679744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281077, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 308, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (328.55, 158.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678444 sequences (100000256 bp) in 2.151 CPU sec, 2.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281527, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 308, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (329.56, 160.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680590 sequences (100000185 bp) in 1.280 CPU sec, 1.284 real sec...
[M::mem_process_seqs] Processed 678114 reads in 126.964 CPU sec, 4.051 real sec
[M::mem_process_seqs] Processed 678226 reads in 78.197 CPU sec, 2.481 real sec
sorting block 16, #reads = 1083076 mem = 536880478 peak = 939679744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281244, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 306, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (328.72, 160.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679978 sequences (100000256 bp) in 2.205 CPU sec, 2.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282176, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 292, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (314.22, 151.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678444 reads in 122.026 CPU sec, 3.881 real sec
[M::process] read 679376 sequences (100000279 bp) in 1.400 CPU sec, 1.424 real sec...
[M::mem_process_seqs] Processed 680590 reads in 78.146 CPU sec, 2.464 real sec
sorting block 17, #reads = 1082780 mem = 536880093 peak = 939679744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281754, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 298, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (318.77, 153.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678488 sequences (100000290 bp) in 2.152 CPU sec, 2.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281683, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 304, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (324.32, 156.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679978 reads in 121.303 CPU sec, 3.863 real sec
sorting block 18, #reads = 1084928 mem = 536880256 peak = 939679744
[M::mem_process_seqs] Processed 679376 reads in 77.416 CPU sec, 2.440 real sec
[M::process] read 678220 sequences (100000171 bp) in 1.469 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280908, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 308, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (326.66, 156.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1083193 mem = 536880512 peak = 995135488
[M::process] read 678100 sequences (100000289 bp) in 2.090 CPU sec, 2.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281571, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 312, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (330.29, 158.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678488 reads in 121.471 CPU sec, 3.862 real sec
[M::process] read 678222 sequences (100000046 bp) in 1.378 CPU sec, 1.391 real sec...
[M::mem_process_seqs] Processed 678220 reads in 77.587 CPU sec, 2.451 real sec
sorting block 20, #reads = 1083873 mem = 536880716 peak = 995135488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281036, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 310, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (328.94, 157.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678360 sequences (100000252 bp) in 2.258 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280917, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 306, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (326.73, 157.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678100 reads in 122.702 CPU sec, 3.902 real sec
[M::mem_process_seqs] Processed 678222 reads in 78.699 CPU sec, 2.486 real sec
[M::process] read 678332 sequences (100000041 bp) in 1.487 CPU sec, 1.518 real sec...
sorting block 21, #reads = 1082888 mem = 536878007 peak = 995135488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281381, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 304, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (324.95, 156.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680666 sequences (100000261 bp) in 2.225 CPU sec, 2.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281773, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 306, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (328.80, 159.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678360 reads in 122.583 CPU sec, 3.912 real sec
[M::mem_process_seqs] Processed 678332 reads in 78.245 CPU sec, 2.478 real sec
[M::process] read 682308 sequences (100000091 bp) in 1.634 CPU sec, 1.682 real sec...
sorting block 22, #reads = 1081830 mem = 536877627 peak = 995135488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282071, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 292, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (317.48, 156.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680832 sequences (100000252 bp) in 2.126 CPU sec, 2.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282969, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 281, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (307.91, 151.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680666 reads in 127.630 CPU sec, 4.061 real sec
sorting block 23, #reads = 1083002 mem = 536877001 peak = 995135488
[M::process] read 680440 sequences (100000166 bp) in 1.429 CPU sec, 1.458 real sec...
[M::mem_process_seqs] Processed 682308 reads in 81.329 CPU sec, 2.567 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282765, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 292, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (314.21, 151.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1083028 mem = 536878696 peak = 1011757056
[M::process] read 680106 sequences (100000015 bp) in 2.186 CPU sec, 2.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282408, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 297, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (317.17, 152.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680832 reads in 125.513 CPU sec, 3.990 real sec
[M::process] read 680002 sequences (100000054 bp) in 1.346 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 680440 reads in 79.441 CPU sec, 2.511 real sec
sorting block 25, #reads = 1083057 mem = 536879245 peak = 1011757056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282489, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 299, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (319.19, 153.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679266 sequences (100000168 bp) in 2.087 CPU sec, 2.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281975, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 301, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (321.49, 154.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679206 sequences (100000202 bp) in 1.285 CPU sec, 1.294 real sec...
[M::mem_process_seqs] Processed 680106 reads in 130.517 CPU sec, 4.152 real sec
[M::mem_process_seqs] Processed 680002 reads in 84.075 CPU sec, 2.663 real sec
sorting block 26, #reads = 1082750 mem = 536879510 peak = 1011757056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281872, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 298, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (316.50, 149.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678804 sequences (100000021 bp) in 2.071 CPU sec, 2.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281655, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 300, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (317.57, 149.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678946 sequences (100000246 bp) in 1.328 CPU sec, 1.337 real sec...
[M::mem_process_seqs] Processed 679266 reads in 130.240 CPU sec, 4.141 real sec
[M::mem_process_seqs] Processed 679206 reads in 80.380 CPU sec, 2.542 real sec
sorting block 27, #reads = 1080343 mem = 536884513 peak = 1011757056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282609, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 301, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (318.67, 149.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682352 sequences (100000251 bp) in 2.265 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281903, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 300, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (320.00, 152.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678804 reads in 128.575 CPU sec, 4.081 real sec
[M::process] read 681284 sequences (100000114 bp) in 1.360 CPU sec, 1.356 real sec...
sorting block 28, #reads = 1082249 mem = 536881241 peak = 1011757056
[M::mem_process_seqs] Processed 678946 reads in 82.276 CPU sec, 2.601 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283050, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 279, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (303.65, 146.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1081332 mem = 536877580 peak = 1056047104
[M::process] read 680730 sequences (100000113 bp) in 2.313 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282999, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 288, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (309.39, 147.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682352 reads in 129.862 CPU sec, 4.123 real sec
[M::process] read 680038 sequences (100000038 bp) in 1.350 CPU sec, 1.344 real sec...
[M::mem_process_seqs] Processed 681284 reads in 82.315 CPU sec, 2.605 real sec
sorting block 30, #reads = 1082148 mem = 536882973 peak = 1056047104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282535, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 292, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (312.38, 149.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680176 sequences (100000186 bp) in 2.236 CPU sec, 2.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282950, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 297, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (316.23, 150.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680730 reads in 128.161 CPU sec, 4.073 real sec
[M::process] read 679382 sequences (100000287 bp) in 1.395 CPU sec, 1.401 real sec...
[M::mem_process_seqs] Processed 680038 reads in 81.048 CPU sec, 2.561 real sec
sorting block 31, #reads = 1081661 mem = 536880455 peak = 1056047104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282260, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 297, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (316.48, 150.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679080 sequences (100000263 bp) in 2.215 CPU sec, 2.343 real sec...
[M::process] read 678532 sequences (100000271 bp) in 1.310 CPU sec, 1.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282408, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 300, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (318.50, 151.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680176 reads in 139.299 CPU sec, 4.430 real sec
sorting block 32, #reads = 1082514 mem = 536879684 peak = 1056047104
[M::mem_process_seqs] Processed 679382 reads in 92.582 CPU sec, 2.935 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282460, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 302, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (320.66, 152.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1078350 mem = 536878869 peak = 1056047104
[M::process] read 678520 sequences (100000025 bp) in 2.184 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281723, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 306, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (323.68, 153.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681598 sequences (100000075 bp) in 1.360 CPU sec, 1.359 real sec...
[M::mem_process_seqs] Processed 679080 reads in 131.989 CPU sec, 4.190 real sec
[M::mem_process_seqs] Processed 678532 reads in 82.372 CPU sec, 2.610 real sec
sorting block 34, #reads = 1080160 mem = 536879426 peak = 1056047104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281498, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 307, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (325.85, 156.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681166 sequences (100000193 bp) in 2.169 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282822, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 283, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (307.30, 148.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680576 sequences (100000164 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_process_seqs] Processed 678520 reads in 133.239 CPU sec, 4.237 real sec
[M::mem_process_seqs] Processed 681598 reads in 85.449 CPU sec, 2.710 real sec
sorting block 35, #reads = 1078387 mem = 536878003 peak = 1056047104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283138, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (309.84, 148.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680068 sequences (100000135 bp) in 2.330 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282498, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 294, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (313.52, 149.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679620 sequences (100000065 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_process_seqs] Processed 681166 reads in 134.810 CPU sec, 4.282 real sec
[M::mem_process_seqs] Processed 680576 reads in 83.348 CPU sec, 2.645 real sec
sorting block 36, #reads = 1081143 mem = 536882621 peak = 1056047104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282356, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 297, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (315.90, 149.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679026 sequences (100000261 bp) in 2.358 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282061, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 299, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (317.07, 149.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680068 reads in 128.554 CPU sec, 4.082 real sec
[M::process] read 678478 sequences (100000163 bp) in 1.339 CPU sec, 1.340 real sec...
sorting block 37, #reads = 1082109 mem = 536878445 peak = 1056047104
[M::mem_process_seqs] Processed 679620 reads in 83.610 CPU sec, 2.648 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282437, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 305, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (321.75, 152.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1081255 mem = 536881559 peak = 1056047104
[M::process] read 678370 sequences (100000228 bp) in 2.243 CPU sec, 2.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281620, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 306, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (323.99, 153.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678500 sequences (100000108 bp) in 1.350 CPU sec, 1.353 real sec...
[M::mem_process_seqs] Processed 679026 reads in 130.482 CPU sec, 4.143 real sec
[M::mem_process_seqs] Processed 678478 reads in 81.527 CPU sec, 2.581 real sec
sorting block 39, #reads = 1080250 mem = 536879878 peak = 1056047104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281438, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 308, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (325.62, 154.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680544 sequences (100000197 bp) in 2.268 CPU sec, 2.393 real sec...
[M::process] read 681730 sequences (100000105 bp) in 1.330 CPU sec, 1.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281582, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 304, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (323.89, 155.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678370 reads in 143.153 CPU sec, 4.552 real sec
[M::mem_process_seqs] Processed 678500 reads in 86.995 CPU sec, 2.760 real sec
sorting block 40, #reads = 1076664 mem = 536878525 peak = 1056047104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282028, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (315.31, 154.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680590 sequences (100000172 bp) in 2.098 CPU sec, 2.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282710, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 285, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (309.00, 149.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680086 sequences (100000067 bp) in 1.340 CPU sec, 1.335 real sec...
[M::mem_process_seqs] Processed 680544 reads in 136.256 CPU sec, 4.333 real sec
[M::mem_process_seqs] Processed 681730 reads in 85.123 CPU sec, 2.699 real sec
sorting block 41, #reads = 1077354 mem = 536879491 peak = 1056047104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282568, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 295, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (316.07, 152.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (426, 4251, 4251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11901)
[M::mem_pestat] mean and std.dev: (2609.80, 1855.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15726)
[M::mem_pestat] skip orientation RR
[M::process] read 679364 sequences (100000053 bp) in 2.205 CPU sec, 2.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282549, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 298, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (318.96, 153.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679022 sequences (100000273 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_process_seqs] Processed 680590 reads in 128.032 CPU sec, 4.069 real sec
sorting block 42, #reads = 1080335 mem = 536878603 peak = 1056047104
[M::mem_process_seqs] Processed 680086 reads in 81.040 CPU sec, 2.566 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281970, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 304, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (324.48, 156.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1081884 mem = 536880853 peak = 1101590528
[M::process] read 678512 sequences (100000000 bp) in 2.246 CPU sec, 2.365 real sec...
[M::process] read 678438 sequences (100000113 bp) in 1.320 CPU sec, 1.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281989, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 307, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (325.81, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679364 reads in 141.195 CPU sec, 4.492 real sec
[M::mem_process_seqs] Processed 679022 reads in 89.991 CPU sec, 2.852 real sec
sorting block 44, #reads = 1079125 mem = 536880372 peak = 1101590528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281753, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 304, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (323.24, 154.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678420 sequences (100000119 bp) in 2.204 CPU sec, 2.321 real sec...
[M::process] read 510928 sequences (75353327 bp) in 1.070 CPU sec, 1.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281806, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 303, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (322.83, 153.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678512 reads in 130.882 CPU sec, 4.165 real sec
[M::mem_process_seqs] Processed 678438 reads in 84.013 CPU sec, 2.667 real sec
sorting block 45, #reads = 1078919 mem = 536879155 peak = 1101590528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281429, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 302, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (321.95, 153.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 212242, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 305, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (325.81, 156.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678420 reads in 111.098 CPU sec, 3.603 real sec
[M::mem_process_seqs] Processed 510928 reads in 63.354 CPU sec, 2.053 real sec
sorting block 46, #reads = 1080544 mem = 536878626 peak = 1106862080
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 51467688
overall: 69730656256 mem 6625.148 user 281.261 sys 950.496 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.659_Fr-N1-BDNA-1_B23KGCJLT4_1.L007\tPU:L007\tSM:659_Fr-N1-BDNA-1_B23KGCJLT4_1\tLB:659_Fr-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_Fr-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 659_Fr-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 950.542 sec; CPU: 6906.412 sec
sorting block 47, #reads = 822128 mem = 408943861 peak = 1126862848
uniq header count 1
merging 48 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2675248896
output reads: 51681394
bam_mem_sort: 48 calls 17.353 user 0.192 sys 17.629 real
bam_write: 48 calls 35.856 user 1.353 sys 37.658 real
execute: 1 calls 9.349 user 0.356 sys 965.181 real
merge_files: 1 calls 9.324 user 0.342 sys 13.561 real
parse_chunk: 765 calls 24.019 user 5.701 sys 30.196 real
read_chunk: 2062 calls 17.706 user 18.897 sys 176.326 real
sort_block: 1 calls 15.090 user 18.507 sys 171.839 real
write_chunk: 1295 calls 9.003 user 0.185 sys 12.889 real
overall: 2553077760 mem 521.378 user 32.879 sys 965.205 real
ls: cannot access '*.cram': No such file or directory