cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_mK-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1544.245 CPU sec, 1251.363 real sec
[M::process] read 685058 sequences (100000291 bp) in 0.930 CPU sec, 0.937 real sec...
[M::process] read 684964 sequences (100000016 bp) in 1.244 CPU sec, 1.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283297, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 312, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (330.80, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684708 sequences (100000170 bp) in 1.175 CPU sec, 1.189 real sec...
[M::process] read 684426 sequences (100000207 bp) in 1.184 CPU sec, 1.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282788, 14, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (332.10, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2303, 2303, 2303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2303, 2303)
[M::mem_pestat] mean and std.dev: (2303.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (2303, 2303)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 685058 reads in 145.554 CPU sec, 4.662 real sec
[M::mem_process_seqs] Processed 684964 reads in 84.645 CPU sec, 2.746 real sec
sorting block 0, #reads = 1087647 mem = 536881258 peak = 600670208
[M::process] read 683836 sequences (100000061 bp) in 1.496 CPU sec, 1.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282423, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (335.25, 164.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683432 sequences (100000034 bp) in 1.150 CPU sec, 1.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282645, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 316, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (336.03, 163.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282209, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 318, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (338.50, 164.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684708 reads in 180.567 CPU sec, 5.788 real sec
[M::mem_process_seqs] Processed 684426 reads in 134.209 CPU sec, 4.330 real sec
[M::mem_process_seqs] Processed 683836 reads in 146.164 CPU sec, 4.747 real sec
sorting block 1, #reads = 1087275 mem = 536882576 peak = 731217920
[M::process] read 683312 sequences (100000163 bp) in 1.795 CPU sec, 1.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282528, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (340.05, 165.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1820, 1820, 1820)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1820, 1820)
[M::mem_pestat] mean and std.dev: (1820.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1820, 1820)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 2, #reads = 1087462 mem = 536881079 peak = 787103744
[M::process] read 683556 sequences (100000262 bp) in 1.399 CPU sec, 1.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282068, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (339.16, 164.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683432 reads in 129.631 CPU sec, 4.132 real sec
[M::mem_process_seqs] Processed 683312 reads in 82.392 CPU sec, 2.605 real sec
[M::process] read 683232 sequences (100000051 bp) in 1.608 CPU sec, 1.691 real sec...
sorting block 3, #reads = 1087023 mem = 536880526 peak = 787103744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282311, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (336.57, 164.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683538 sequences (100000046 bp) in 1.676 CPU sec, 1.793 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282225, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (339.66, 166.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684240 sequences (100000134 bp) in 1.244 CPU sec, 1.242 real sec...
[M::mem_process_seqs] Processed 683556 reads in 130.008 CPU sec, 4.145 real sec
[M::mem_process_seqs] Processed 683232 reads in 82.562 CPU sec, 2.621 real sec
sorting block 4, #reads = 1086643 mem = 536877521 peak = 787103744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282477, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (338.29, 165.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685158 sequences (100000230 bp) in 1.797 CPU sec, 1.914 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282601, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (337.12, 162.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684302 sequences (100000238 bp) in 1.284 CPU sec, 1.287 real sec...
[M::mem_process_seqs] Processed 683538 reads in 129.257 CPU sec, 4.121 real sec
[M::mem_process_seqs] Processed 684240 reads in 81.251 CPU sec, 2.581 real sec
sorting block 5, #reads = 1086694 mem = 536878322 peak = 787103744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282671, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (334.00, 163.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684428 sequences (100000168 bp) in 2.010 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282408, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (338.02, 164.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683878 sequences (100000209 bp) in 1.280 CPU sec, 1.279 real sec...
[M::mem_process_seqs] Processed 685158 reads in 129.932 CPU sec, 4.137 real sec
sorting block 6, #reads = 1087726 mem = 536878120 peak = 793554944
[M::mem_process_seqs] Processed 684302 reads in 81.983 CPU sec, 2.601 real sec
sorting block 7, #reads = 1088019 mem = 536877231 peak = 917204992
[M::process] read 683762 sequences (100000260 bp) in 1.880 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282421, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 320, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (339.73, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683592 sequences (100000202 bp) in 1.320 CPU sec, 1.319 real sec...
[M::mem_process_seqs] Processed 683878 reads in 75.765 CPU sec, 2.397 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282701, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 319, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (339.35, 165.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282377, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (340.68, 166.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684428 reads in 200.348 CPU sec, 6.381 real sec
[M::process] read 683918 sequences (100000069 bp) in 1.521 CPU sec, 1.536 real sec...
sorting block 8, #reads = 1087683 mem = 536879665 peak = 917204992
[M::mem_process_seqs] Processed 683762 reads in 94.661 CPU sec, 3.020 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282513, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (338.63, 165.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1087157 mem = 536879219 peak = 917204992
[M::process] read 683512 sequences (100000212 bp) in 2.594 CPU sec, 2.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282365, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 314, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (337.90, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683592 reads in 132.525 CPU sec, 4.222 real sec
[M::mem_process_seqs] Processed 683918 reads in 85.180 CPU sec, 2.690 real sec
[M::process] read 684762 sequences (100000035 bp) in 1.903 CPU sec, 1.982 real sec...
sorting block 10, #reads = 1085769 mem = 536878490 peak = 917204992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282341, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 315, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (339.22, 167.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684778 sequences (100000174 bp) in 2.050 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282552, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 315, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (334.43, 163.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683512 reads in 133.259 CPU sec, 4.258 real sec
[M::mem_process_seqs] Processed 684762 reads in 85.022 CPU sec, 2.693 real sec
[M::process] read 685318 sequences (100000105 bp) in 1.795 CPU sec, 1.854 real sec...
sorting block 11, #reads = 1085458 mem = 536879902 peak = 917204992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282664, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 317, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (334.36, 160.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684706 sequences (100000131 bp) in 2.392 CPU sec, 2.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282863, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 314, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (332.87, 161.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684778 reads in 129.745 CPU sec, 4.147 real sec
[M::mem_process_seqs] Processed 685318 reads in 83.906 CPU sec, 2.657 real sec
sorting block 12, #reads = 1087845 mem = 536877271 peak = 917204992
[M::process] read 684084 sequences (100000006 bp) in 2.039 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283156, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (335.92, 163.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683976 sequences (100000063 bp) in 2.084 CPU sec, 2.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282539, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (338.95, 164.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684706 reads in 130.599 CPU sec, 4.171 real sec
sorting block 13, #reads = 1087496 mem = 536882314 peak = 917204992
[M::mem_process_seqs] Processed 684084 reads in 83.780 CPU sec, 2.651 real sec
[M::process] read 683242 sequences (100000190 bp) in 1.752 CPU sec, 1.806 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282692, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (340.23, 165.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1087456 mem = 536878609 peak = 924962816
[M::process] read 682970 sequences (100000204 bp) in 2.149 CPU sec, 2.269 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282140, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 323, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (343.62, 167.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683976 reads in 132.801 CPU sec, 4.227 real sec
[M::process] read 683068 sequences (100000069 bp) in 1.363 CPU sec, 1.370 real sec...
[M::mem_process_seqs] Processed 683242 reads in 84.826 CPU sec, 2.686 real sec
sorting block 15, #reads = 1086635 mem = 536878918 peak = 924962816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281880, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (343.69, 167.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683184 sequences (100000035 bp) in 2.758 CPU sec, 2.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282683, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (342.75, 167.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682970 reads in 131.374 CPU sec, 4.193 real sec
[M::mem_process_seqs] Processed 683068 reads in 84.308 CPU sec, 2.666 real sec
[M::process] read 685710 sequences (100000131 bp) in 2.176 CPU sec, 2.309 real sec...
sorting block 16, #reads = 1087405 mem = 536879022 peak = 924962816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282261, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (341.64, 167.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685624 sequences (100000142 bp) in 2.167 CPU sec, 2.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282945, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 310, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (330.17, 160.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683184 reads in 132.547 CPU sec, 4.238 real sec
[M::mem_process_seqs] Processed 685710 reads in 83.984 CPU sec, 2.656 real sec
sorting block 17, #reads = 1086891 mem = 536877636 peak = 924962816
[M::process] read 685548 sequences (100000150 bp) in 2.210 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282740, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 308, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (328.17, 159.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685194 sequences (100000214 bp) in 2.121 CPU sec, 2.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282910, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 309, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (329.58, 161.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685624 reads in 127.638 CPU sec, 4.079 real sec
sorting block 18, #reads = 1088287 mem = 536877818 peak = 924962816
[M::mem_process_seqs] Processed 685548 reads in 82.212 CPU sec, 2.601 real sec
[M::process] read 684336 sequences (100000064 bp) in 1.929 CPU sec, 2.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282540, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 310, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (330.94, 162.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1087980 mem = 536881473 peak = 924962816
[M::process] read 683956 sequences (100000042 bp) in 2.037 CPU sec, 2.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282666, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (336.07, 164.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685194 reads in 126.803 CPU sec, 4.042 real sec
[M::mem_process_seqs] Processed 684336 reads in 81.939 CPU sec, 2.593 real sec
[M::process] read 682982 sequences (100000047 bp) in 1.780 CPU sec, 1.845 real sec...
sorting block 20, #reads = 1087874 mem = 536880968 peak = 924962816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282333, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (336.25, 164.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682452 sequences (100000189 bp) in 2.135 CPU sec, 2.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281875, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (340.21, 165.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683956 reads in 128.453 CPU sec, 4.089 real sec
[M::mem_process_seqs] Processed 682982 reads in 82.511 CPU sec, 2.604 real sec
[M::process] read 682320 sequences (100000196 bp) in 1.588 CPU sec, 1.635 real sec...
sorting block 21, #reads = 1087081 mem = 536879791 peak = 924962816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281763, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (342.76, 165.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684156 sequences (100000118 bp) in 2.241 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281736, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (343.77, 166.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682452 reads in 128.729 CPU sec, 4.102 real sec
[M::mem_process_seqs] Processed 682320 reads in 82.905 CPU sec, 2.620 real sec
sorting block 22, #reads = 1085985 mem = 536884273 peak = 924962816
[M::process] read 687392 sequences (100000205 bp) in 1.676 CPU sec, 1.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282710, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 314, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (337.23, 166.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 23, #reads = 1085686 mem = 536883486 peak = 924962816
[M::process] read 686692 sequences (100000002 bp) in 2.035 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284574, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 302, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (326.10, 162.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684156 reads in 134.187 CPU sec, 4.271 real sec
[M::process] read 686310 sequences (100000239 bp) in 1.406 CPU sec, 1.405 real sec...
[M::mem_process_seqs] Processed 687392 reads in 86.642 CPU sec, 2.735 real sec
sorting block 24, #reads = 1086774 mem = 536880945 peak = 924962816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283762, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 305, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (326.91, 161.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685564 sequences (100000027 bp) in 2.269 CPU sec, 2.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283775, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 305, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (326.29, 159.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685368 sequences (100000201 bp) in 1.370 CPU sec, 1.376 real sec...
[M::mem_process_seqs] Processed 686692 reads in 132.489 CPU sec, 4.205 real sec
[M::mem_process_seqs] Processed 686310 reads in 84.194 CPU sec, 2.667 real sec
sorting block 25, #reads = 1087734 mem = 536879454 peak = 924962816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283669, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 307, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (326.51, 158.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684862 sequences (100000060 bp) in 2.232 CPU sec, 2.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282922, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 309, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (327.78, 158.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684538 sequences (100000107 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_process_seqs] Processed 685564 reads in 134.061 CPU sec, 4.254 real sec
[M::mem_process_seqs] Processed 685368 reads in 84.372 CPU sec, 2.673 real sec
sorting block 26, #reads = 1087205 mem = 536879699 peak = 924962816
[M::process] read 684148 sequences (100000146 bp) in 2.294 CPU sec, 2.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282883, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (327.50, 157.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683540 sequences (100000163 bp) in 1.410 CPU sec, 1.417 real sec...
[M::mem_process_seqs] Processed 684538 reads in 78.789 CPU sec, 2.485 real sec
sorting block 27, #reads = 1086252 mem = 536880290 peak = 924962816
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282909, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 309, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (329.65, 160.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281830, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (327.38, 157.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686068 sequences (100000051 bp) in 1.571 CPU sec, 1.580 real sec...
[M::mem_process_seqs] Processed 684862 reads in 210.399 CPU sec, 6.685 real sec
[M::mem_process_seqs] Processed 684148 reads in 100.043 CPU sec, 3.193 real sec
sorting block 28, #reads = 1085866 mem = 536880483 peak = 968167424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282232, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 310, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (331.45, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686142 sequences (100000078 bp) in 2.379 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283046, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 305, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (326.44, 159.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686198 sequences (100000165 bp) in 1.430 CPU sec, 1.428 real sec...
[M::mem_process_seqs] Processed 683540 reads in 137.225 CPU sec, 4.353 real sec
[M::mem_process_seqs] Processed 686068 reads in 86.335 CPU sec, 2.731 real sec
sorting block 29, #reads = 1084423 mem = 536878637 peak = 968167424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284100, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 306, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (324.88, 157.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686088 sequences (100000147 bp) in 2.350 CPU sec, 2.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283747, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 304, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (321.83, 154.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686142 reads in 133.187 CPU sec, 4.248 real sec
sorting block 30, #reads = 1084804 mem = 536879076 peak = 976801792
[M::mem_process_seqs] Processed 686198 reads in 85.083 CPU sec, 2.686 real sec
[M::process] read 685806 sequences (100000165 bp) in 2.244 CPU sec, 2.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284047, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 304, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (322.56, 155.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1087242 mem = 536878187 peak = 976801792
[M::process] read 685272 sequences (100000003 bp) in 2.598 CPU sec, 2.774 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283367, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 307, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (325.09, 156.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686088 reads in 135.109 CPU sec, 4.329 real sec
[M::mem_process_seqs] Processed 685806 reads in 86.072 CPU sec, 2.726 real sec
sorting block 32, #reads = 1086149 mem = 536882313 peak = 976801792
[M::process] read 684490 sequences (100000294 bp) in 2.596 CPU sec, 2.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283903, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (326.73, 157.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282448, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 311, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (329.67, 158.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684078 sequences (100000194 bp) in 2.123 CPU sec, 2.204 real sec...
[M::mem_process_seqs] Processed 685272 reads in 135.815 CPU sec, 4.351 real sec
[M::mem_process_seqs] Processed 684490 reads in 87.181 CPU sec, 2.768 real sec
sorting block 33, #reads = 1086713 mem = 536877379 peak = 976801792
[M::process] read 683728 sequences (100000002 bp) in 2.445 CPU sec, 2.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282475, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (332.02, 159.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282417, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (333.24, 161.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684054 sequences (100000083 bp) in 2.109 CPU sec, 2.185 real sec...
[M::mem_process_seqs] Processed 684078 reads in 137.557 CPU sec, 4.401 real sec
[M::mem_process_seqs] Processed 683728 reads in 87.873 CPU sec, 2.791 real sec
sorting block 34, #reads = 1085212 mem = 536882077 peak = 976801792
[M::process] read 686510 sequences (100000289 bp) in 2.440 CPU sec, 2.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282447, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 312, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (334.61, 164.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1085656 mem = 536878444 peak = 976801792
[M::process] read 686738 sequences (100000072 bp) in 1.872 CPU sec, 1.950 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283921, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 308, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (328.20, 161.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684054 reads in 143.936 CPU sec, 4.592 real sec
[M::mem_process_seqs] Processed 686510 reads in 87.888 CPU sec, 2.780 real sec
[M::process] read 685810 sequences (100000255 bp) in 2.031 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283690, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 303, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (323.58, 157.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1083502 mem = 536879578 peak = 976801792
[M::process] read 685342 sequences (100000172 bp) in 2.275 CPU sec, 2.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283715, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 306, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (323.86, 155.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686738 reads in 135.814 CPU sec, 4.334 real sec
[M::mem_process_seqs] Processed 685810 reads in 86.348 CPU sec, 2.731 real sec
[M::process] read 684872 sequences (100000224 bp) in 1.927 CPU sec, 2.032 real sec...
sorting block 37, #reads = 1087422 mem = 536878506 peak = 976801792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283655, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 307, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (325.28, 156.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684266 sequences (100000245 bp) in 1.998 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283011, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 309, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (326.62, 156.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684036 sequences (100000205 bp) in 1.290 CPU sec, 1.288 real sec...
[M::mem_process_seqs] Processed 685342 reads in 137.444 CPU sec, 4.375 real sec
[M::mem_process_seqs] Processed 684872 reads in 88.712 CPU sec, 2.810 real sec
sorting block 38, #reads = 1087058 mem = 536880791 peak = 984928256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282981, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (331.48, 159.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683914 sequences (100000071 bp) in 2.042 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282518, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 313, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (331.21, 158.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684054 sequences (100000072 bp) in 1.380 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 684266 reads in 138.919 CPU sec, 4.421 real sec
sorting block 39, #reads = 1084746 mem = 536879120 peak = 987439104
[M::mem_process_seqs] Processed 684036 reads in 86.585 CPU sec, 2.750 real sec
sorting block 40, #reads = 1084786 mem = 536879630 peak = 1048104960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282961, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 314, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (332.59, 159.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686170 sequences (100000013 bp) in 2.048 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282795, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 316, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (334.85, 161.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687030 sequences (100000024 bp) in 1.600 CPU sec, 1.599 real sec...
[M::mem_process_seqs] Processed 683914 reads in 137.070 CPU sec, 4.349 real sec
[M::mem_process_seqs] Processed 684054 reads in 87.202 CPU sec, 2.753 real sec
sorting block 41, #reads = 1085667 mem = 536879752 peak = 1048104960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283158, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 306, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (329.57, 164.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687290 sequences (100000081 bp) in 2.459 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283840, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 304, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (327.50, 163.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686170 reads in 139.138 CPU sec, 4.416 real sec
[M::process] read 686308 sequences (100000158 bp) in 1.709 CPU sec, 1.727 real sec...
[M::mem_process_seqs] Processed 687030 reads in 87.077 CPU sec, 2.744 real sec
sorting block 42, #reads = 1083412 mem = 536885474 peak = 1048104960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284360, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 302, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (323.91, 160.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685594 sequences (100000100 bp) in 2.382 CPU sec, 2.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283664, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 306, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (328.14, 161.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687290 reads in 135.352 CPU sec, 4.307 real sec
[M::process] read 684688 sequences (100000055 bp) in 1.482 CPU sec, 1.488 real sec...
[M::mem_process_seqs] Processed 686308 reads in 86.062 CPU sec, 2.717 real sec
sorting block 43, #reads = 1087984 mem = 536878025 peak = 1048104960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284006, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 309, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (331.94, 164.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683790 sequences (100000211 bp) in 2.290 CPU sec, 2.447 real sec...
[M::process] read 683374 sequences (100000250 bp) in 1.390 CPU sec, 1.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283762, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 308, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (327.08, 156.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685594 reads in 148.185 CPU sec, 4.715 real sec
sorting block 44, #reads = 1087040 mem = 536880783 peak = 1048104960
[M::mem_process_seqs] Processed 684688 reads in 90.562 CPU sec, 2.876 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282861, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 312, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (330.66, 157.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1081407 mem = 536883129 peak = 1070198784
[M::process] read 683166 sequences (100000228 bp) in 2.384 CPU sec, 2.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282458, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 315, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (332.75, 157.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 658564 sequences (96436882 bp) in 1.380 CPU sec, 1.376 real sec...
[M::mem_process_seqs] Processed 683790 reads in 132.494 CPU sec, 4.204 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282470, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 316, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (334.38, 159.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683166 reads in 72.919 CPU sec, 2.539 real sec
sorting block 46, #reads = 1086278 mem = 536877989 peak = 1070198784
[M::mem_process_seqs] Processed 683374 reads in 179.407 CPU sec, 5.916 real sec
sorting block 47, #reads = 1085611 mem = 536879648 peak = 1114030080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 272162, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (337.92, 161.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 658564 reads in 82.946 CPU sec, 2.731 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 52691072
overall: 69834313728 mem 6925.658 user 243.382 sys 1439.073 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.659_mK-N1-BDNA-1_B23KGCJLT4_1.L006\tPU:L006\tSM:659_mK-N1-BDNA-1_B23KGCJLT4_1\tLB:659_mK-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_mK-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 659_mK-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 1439.115 sec; CPU: 7169.043 sec
sorting block 48, #reads = 792782 mem = 392509101 peak = 1114030080
uniq header count 1
merging 49 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2791664915
output reads: 52942902
bam_mem_sort: 49 calls 20.741 user 0.402 sys 21.247 real
bam_write: 49 calls 24.939 user 0.838 sys 25.960 real
execute: 1 calls 9.894 user 0.531 sys 1454.470 real
merge_files: 1 calls 9.879 user 0.506 sys 14.348 real
parse_chunk: 780 calls 26.014 user 7.207 sys 33.805 real
read_chunk: 2104 calls 20.325 user 19.290 sys 188.015 real
sort_block: 1 calls 17.582 user 18.861 sys 183.140 real
write_chunk: 1322 calls 9.516 user 0.345 sys 13.628 real
overall: 2490261504 mem 560.503 user 33.390 sys 1454.500 real
ls: cannot access '*.cram': No such file or directory