.command.err - Workdir - DAQ:SENTIEON_BWAMEM (NA12878_c_0002_gDNA_0003_B23KGCJLT4_1;L005;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/5e/0c8d18cbcdc872a0dd19625a035cf4/.command.err
Size: 48.2 KB
Task: DAQ:SENTIEON_BWAMEM (NA12878_c_0002_gDNA_0003_B23KGCJLT4_1;L005;0001)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0003_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1711.112 CPU sec, 484.098 real sec
[M::process] read 681334 sequences (100000056 bp) in 0.900 CPU sec, 0.901 real sec...
[M::process] read 679836 sequences (100000132 bp) in 1.100 CPU sec, 1.108 real sec...
[M::process] read 680750 sequences (100000178 bp) in 1.128 CPU sec, 1.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282743, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (334.68, 157.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680716 sequences (100000106 bp) in 1.079 CPU sec, 1.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283270, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (339.80, 161.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282059, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 186, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 310)
[M::mem_pestat] mean and std.dev: (152.17, 51.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 362)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (345.37, 162.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 681334 reads in 205.768 CPU sec, 6.572 real sec
[M::mem_process_seqs] Processed 680750 reads in 137.786 CPU sec, 4.435 real sec
[M::mem_process_seqs] Processed 679836 reads in 149.447 CPU sec, 4.832 real sec
sorting block 0, #reads = 1067559 mem = 536878527 peak = 594006016
[M::process] read 680366 sequences (100000051 bp) in 1.608 CPU sec, 1.753 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282641, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (338.83, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 182, 185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 237)
[M::mem_pestat] mean and std.dev: (167.50, 22.06)
[M::mem_pestat] low and high boundaries for proper pairs: (79, 263)
[M::mem_pestat] skip orientation RR
[M::process] read 680390 sequences (100000016 bp) in 1.202 CPU sec, 1.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282439, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 320, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (341.45, 163.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680964 sequences (100000173 bp) in 1.030 CPU sec, 1.034 real sec...
[M::mem_process_seqs] Processed 680716 reads in 128.634 CPU sec, 4.091 real sec
sorting block 1, #reads = 1067248 mem = 536879268 peak = 808415232
[M::mem_process_seqs] Processed 680366 reads in 80.820 CPU sec, 2.556 real sec
sorting block 2, #reads = 1067643 mem = 536878585 peak = 883167232
[M::process] read 679872 sequences (100000158 bp) in 1.542 CPU sec, 1.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282771, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 316, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (338.48, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681050 sequences (100000171 bp) in 1.214 CPU sec, 1.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282907, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (340.25, 160.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680390 reads in 125.743 CPU sec, 3.998 real sec
[M::mem_process_seqs] Processed 680964 reads in 78.634 CPU sec, 2.513 real sec
sorting block 3, #reads = 1067751 mem = 536878892 peak = 883167232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282633, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (348.70, 165.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680734 sequences (100000235 bp) in 1.687 CPU sec, 1.788 real sec...
[M::process] read 681240 sequences (100000170 bp) in 1.140 CPU sec, 1.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282687, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 233, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (249.18, 135.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (338.01, 161.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 679872 reads in 127.348 CPU sec, 4.054 real sec
[M::mem_process_seqs] Processed 681050 reads in 80.767 CPU sec, 2.571 real sec
sorting block 4, #reads = 1068181 mem = 536883781 peak = 883167232
[M::process] read 681072 sequences (100000277 bp) in 1.597 CPU sec, 1.706 real sec...
[M::process] read 679968 sequences (100000149 bp) in 1.160 CPU sec, 1.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282894, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 181, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273)
[M::mem_pestat] mean and std.dev: (243.70, 168.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1662)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 312, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (335.75, 162.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 681240 reads in 70.335 CPU sec, 2.229 real sec
sorting block 5, #reads = 1067755 mem = 536878419 peak = 883167232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282713, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 207, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (113, 348)
[M::mem_pestat] mean and std.dev: (207.20, 41.58)
[M::mem_pestat] low and high boundaries for proper pairs: (41, 395)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 318, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (338.57, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282869, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 222, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (179.12, 51.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 694)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 315, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (337.29, 162.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (350, 490, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217)
[M::mem_pestat] mean and std.dev: (462.12, 158.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1506)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 679526 sequences (100000211 bp) in 1.221 CPU sec, 1.228 real sec...
[M::mem_process_seqs] Processed 680734 reads in 200.213 CPU sec, 6.373 real sec
[M::mem_process_seqs] Processed 681072 reads in 94.916 CPU sec, 3.038 real sec
sorting block 6, #reads = 1067912 mem = 536879408 peak = 883167232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282963, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (345.31, 162.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 174, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (235.09, 102.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RR
[M::process] read 680158 sequences (100000108 bp) in 1.960 CPU sec, 2.084 real sec...
[M::process] read 680452 sequences (100000210 bp) in 1.170 CPU sec, 1.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282204, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (350.39, 166.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679968 reads in 128.472 CPU sec, 4.084 real sec
[M::mem_process_seqs] Processed 679526 reads in 81.133 CPU sec, 2.582 real sec
sorting block 7, #reads = 1067428 mem = 536879535 peak = 883167232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283068, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (341.75, 162.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680524 sequences (100000157 bp) in 1.896 CPU sec, 2.010 real sec...
[M::process] read 680850 sequences (100000230 bp) in 1.220 CPU sec, 1.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282716, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 228, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570)
[M::mem_pestat] mean and std.dev: (193.47, 66.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 319, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (341.05, 163.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 680158 reads in 128.693 CPU sec, 4.091 real sec
sorting block 8, #reads = 1067282 mem = 536878702 peak = 893595648
[M::mem_process_seqs] Processed 680452 reads in 81.079 CPU sec, 2.579 real sec
sorting block 9, #reads = 1067130 mem = 536878582 peak = 927170560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283204, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (340.00, 164.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 354, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (268.55, 102.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation RR
[M::process] read 679696 sequences (100000119 bp) in 1.914 CPU sec, 2.035 real sec...
[M::process] read 679650 sequences (100000035 bp) in 1.220 CPU sec, 1.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283037, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (338.90, 162.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680524 reads in 128.369 CPU sec, 4.080 real sec
[M::mem_process_seqs] Processed 680850 reads in 80.544 CPU sec, 2.558 real sec
sorting block 10, #reads = 1067920 mem = 536880271 peak = 927170560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282033, 0, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 167, 169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (97, 217)
[M::mem_pestat] mean and std.dev: (155.08, 25.05)
[M::mem_pestat] low and high boundaries for proper pairs: (55, 255)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (347.43, 165.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 179, 212)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 334)
[M::mem_pestat] mean and std.dev: (176.55, 35.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 395)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680522 sequences (100000058 bp) in 2.014 CPU sec, 2.151 real sec...
[M::process] read 680846 sequences (100000161 bp) in 1.260 CPU sec, 1.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282924, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (349.07, 166.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679696 reads in 127.634 CPU sec, 4.054 real sec
[M::mem_process_seqs] Processed 679650 reads in 80.344 CPU sec, 2.549 real sec
sorting block 11, #reads = 1067908 mem = 536880012 peak = 927170560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283347, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 175, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 523)
[M::mem_pestat] mean and std.dev: (200.18, 92.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 652)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (340.47, 163.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680702 sequences (100000071 bp) in 2.007 CPU sec, 2.126 real sec...
[M::process] read 681866 sequences (100000108 bp) in 1.270 CPU sec, 1.271 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282628, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 313, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (336.27, 162.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680522 reads in 128.774 CPU sec, 4.090 real sec
sorting block 12, #reads = 1068037 mem = 536879906 peak = 927170560
[M::mem_process_seqs] Processed 680846 reads in 81.592 CPU sec, 2.588 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283190, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 312, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (336.88, 163.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1067672 mem = 536878341 peak = 927170560
[M::process] read 680374 sequences (100000014 bp) in 2.109 CPU sec, 2.254 real sec...
[M::process] read 681500 sequences (100000276 bp) in 1.300 CPU sec, 1.299 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283401, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 311, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (334.18, 162.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680702 reads in 132.354 CPU sec, 4.203 real sec
[M::mem_process_seqs] Processed 681866 reads in 84.415 CPU sec, 2.674 real sec
sorting block 14, #reads = 1067472 mem = 536879618 peak = 927170560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283214, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (345.23, 164.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 209, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 368)
[M::mem_pestat] mean and std.dev: (217.08, 70.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 499)
[M::mem_pestat] skip orientation RR
[M::process] read 681964 sequences (100000112 bp) in 2.327 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283611, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 315, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (336.59, 162.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680374 reads in 133.597 CPU sec, 4.243 real sec
[M::process] read 681358 sequences (100000197 bp) in 1.437 CPU sec, 1.444 real sec...
[M::mem_process_seqs] Processed 681500 reads in 83.949 CPU sec, 2.659 real sec
sorting block 15, #reads = 1067993 mem = 536880647 peak = 927170560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283400, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 246, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744)
[M::mem_pestat] mean and std.dev: (273.17, 186.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2281)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 308, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (328.06, 155.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681540 sequences (100000141 bp) in 2.359 CPU sec, 2.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283263, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (327.47, 153.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681454 sequences (100000035 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_process_seqs] Processed 681964 reads in 136.708 CPU sec, 4.341 real sec
[M::mem_process_seqs] Processed 681358 reads in 86.532 CPU sec, 2.733 real sec
sorting block 16, #reads = 1067313 mem = 536881393 peak = 927170560
[M::process] read 680590 sequences (100000181 bp) in 2.739 CPU sec, 2.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284068, 0, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 196, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552)
[M::mem_pestat] mean and std.dev: (186.00, 75.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 696)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 318, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (337.86, 161.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 193, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (258.37, 141.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681454 reads in 76.124 CPU sec, 2.406 real sec
sorting block 17, #reads = 1066030 mem = 536879131 peak = 927170560
[M::process] read 681922 sequences (100000189 bp) in 1.786 CPU sec, 1.844 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283632, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 309, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (329.94, 156.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283534, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 180, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106)
[M::mem_pestat] mean and std.dev: (221.17, 137.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1427)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (340.43, 160.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 681540 reads in 214.829 CPU sec, 6.819 real sec
[M::mem_process_seqs] Processed 680590 reads in 102.911 CPU sec, 3.259 real sec
[M::process] read 681496 sequences (100000002 bp) in 2.152 CPU sec, 2.225 real sec...
sorting block 18, #reads = 1066667 mem = 536878719 peak = 941707264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283691, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 308, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (327.83, 155.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681432 sequences (100000099 bp) in 2.714 CPU sec, 2.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283387, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 310, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (330.08, 156.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681922 reads in 140.689 CPU sec, 4.502 real sec
[M::mem_process_seqs] Processed 681496 reads in 89.338 CPU sec, 2.831 real sec
sorting block 19, #reads = 1065712 mem = 536879137 peak = 941707264
[M::process] read 681686 sequences (100000036 bp) in 1.796 CPU sec, 1.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283106, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (332.36, 158.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1067009 mem = 536879928 peak = 941707264
[M::process] read 679834 sequences (100000121 bp) in 2.403 CPU sec, 2.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283557, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (335.21, 161.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681432 reads in 146.462 CPU sec, 4.675 real sec
[M::mem_process_seqs] Processed 681686 reads in 96.192 CPU sec, 3.042 real sec
[M::process] read 680236 sequences (100000002 bp) in 1.932 CPU sec, 2.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283066, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (342.97, 159.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1066529 mem = 536878219 peak = 941707264
[M::process] read 680850 sequences (100000016 bp) in 2.435 CPU sec, 2.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282854, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (339.32, 158.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679834 reads in 138.222 CPU sec, 4.416 real sec
[M::mem_process_seqs] Processed 680236 reads in 89.481 CPU sec, 2.841 real sec
[M::process] read 680498 sequences (100000208 bp) in 1.753 CPU sec, 1.840 real sec...
sorting block 22, #reads = 1065505 mem = 536880303 peak = 941707264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283578, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 246, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (228.50, 93.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 311, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (329.34, 153.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680820 sequences (100000049 bp) in 2.569 CPU sec, 2.730 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283016, 0, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 153, 187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (85, 255)
[M::mem_pestat] mean and std.dev: (165.10, 32.49)
[M::mem_pestat] low and high boundaries for proper pairs: (35, 295)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 314, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (333.38, 156.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 222, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (220.19, 76.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680850 reads in 142.137 CPU sec, 4.556 real sec
[M::mem_process_seqs] Processed 680498 reads in 91.980 CPU sec, 2.916 real sec
sorting block 23, #reads = 1067077 mem = 536878600 peak = 941707264
[M::process] read 681994 sequences (100000010 bp) in 2.057 CPU sec, 2.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283479, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 178, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191)
[M::mem_pestat] mean and std.dev: (240.09, 178.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1536)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (334.52, 159.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 210, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (238.15, 90.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680012 sequences (100000147 bp) in 2.310 CPU sec, 2.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283558, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 172, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (205.44, 95.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (336.52, 164.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 680820 reads in 148.344 CPU sec, 4.743 real sec
sorting block 24, #reads = 1066881 mem = 536878632 peak = 941707264
[M::process] read 681088 sequences (100000288 bp) in 1.547 CPU sec, 1.579 real sec...
[M::mem_process_seqs] Processed 681994 reads in 96.117 CPU sec, 3.039 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283266, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (250.40, 107.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 333, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (351.12, 167.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 158, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (185.18, 90.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 25, #reads = 1064422 mem = 536879459 peak = 997076992
[M::process] read 680758 sequences (100000170 bp) in 2.524 CPU sec, 2.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283926, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 318, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (339.00, 162.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 411, 1370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3704)
[M::mem_pestat] mean and std.dev: (586.82, 499.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4871)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680012 reads in 143.037 CPU sec, 4.566 real sec
[M::process] read 681006 sequences (100000136 bp) in 1.489 CPU sec, 1.505 real sec...
[M::mem_process_seqs] Processed 681088 reads in 91.862 CPU sec, 2.912 real sec
sorting block 26, #reads = 1065685 mem = 536878174 peak = 997076992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282994, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 201, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206)
[M::mem_pestat] mean and std.dev: (338.18, 266.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1551)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (336.82, 161.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 543384 sequences (79842460 bp) in 2.127 CPU sec, 2.269 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282831, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (332.87, 159.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680758 reads in 139.410 CPU sec, 4.494 real sec
[M::mem_process_seqs] Processed 681006 reads in 87.363 CPU sec, 2.819 real sec
sorting block 27, #reads = 1066926 mem = 536879048 peak = 997076992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 225893, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 209, 218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 324)
[M::mem_pestat] mean and std.dev: (189.70, 35.17)
[M::mem_pestat] low and high boundaries for proper pairs: (6, 377)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 312, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (334.72, 160.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 250, 742)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1880)
[M::mem_pestat] mean and std.dev: (492.73, 486.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2449)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 543384 reads in 60.911 CPU sec, 2.037 real sec
sorting block 28, #reads = 1066442 mem = 536877068 peak = 997076992
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 31178880
overall: 69727207424 mem 4814.256 user 286.716 sys 600.822 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0003_B23KGCJLT4_1.L005\tPU:L005\tSM:NA12878_c_0002_gDNA_0003_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0003_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0003_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0003_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 600.860 sec; CPU: 5100.975 sec
sorting block 29, #reads = 383485 mem = 193081701 peak = 997076992
uniq header count 1
merging 30 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 1699385910
output reads: 31328574
bam_mem_sort: 30 calls 14.074 user 0.201 sys 14.329 real
bam_write: 30 calls 10.236 user 0.281 sys 10.580 real
execute: 1 calls 9.635 user 0.377 sys 611.767 real
merge_files: 1 calls 9.624 user 0.348 sys 10.566 real
parse_chunk: 470 calls 17.299 user 4.324 sys 21.875 real
read_chunk: 1270 calls 10.653 user 10.799 sys 114.175 real
sort_block: 1 calls 7.784 user 10.218 sys 109.615 real
write_chunk: 798 calls 9.177 user 0.234 sys 9.934 real
overall: 2385895424 mem 392.557 user 21.036 sys 611.790 real
ls: cannot access '*.cram': No such file or directory