cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0002_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1574.774 CPU sec, 654.381 real sec
[M::process] read 691176 sequences (100000283 bp) in 0.929 CPU sec, 0.934 real sec...
[M::process] read 689652 sequences (100000186 bp) in 1.180 CPU sec, 1.183 real sec...
[M::process] read 689274 sequences (100000070 bp) in 1.160 CPU sec, 1.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286051, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 201, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362)
[M::mem_pestat] mean and std.dev: (183.50, 51.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 440)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 274, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (295.77, 145.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688412 sequences (100000266 bp) in 1.109 CPU sec, 1.110 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286535, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (302.55, 147.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285670, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (304.69, 148.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691176 reads in 197.982 CPU sec, 6.329 real sec
[M::mem_process_seqs] Processed 689652 reads in 130.477 CPU sec, 4.216 real sec
[M::mem_process_seqs] Processed 689274 reads in 141.894 CPU sec, 4.601 real sec
sorting block 0, #reads = 1076910 mem = 536877801 peak = 603447296
[M::process] read 687916 sequences (100000139 bp) in 1.660 CPU sec, 1.807 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285917, 0, 20)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 285, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (307.40, 149.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 482, 645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405)
[M::mem_pestat] mean and std.dev: (349.62, 165.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1785)
[M::mem_pestat] skip orientation RR
[M::process] read 688170 sequences (100000292 bp) in 1.254 CPU sec, 1.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285511, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (310.67, 151.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (105, 213, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450)
[M::mem_pestat] mean and std.dev: (185.50, 75.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688412 reads in 121.521 CPU sec, 3.864 real sec
sorting block 1, #reads = 1075997 mem = 536877455 peak = 884989952
[M::process] read 686540 sequences (100000296 bp) in 1.154 CPU sec, 1.171 real sec...
[M::mem_process_seqs] Processed 687916 reads in 77.315 CPU sec, 2.435 real sec
sorting block 2, #reads = 1075412 mem = 536882569 peak = 884989952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285543, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 286, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (308.89, 151.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 133, 165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 231)
[M::mem_pestat] mean and std.dev: (136.20, 20.33)
[M::mem_pestat] low and high boundaries for proper pairs: (33, 264)
[M::mem_pestat] skip orientation RR
[M::process] read 686088 sequences (100000224 bp) in 1.382 CPU sec, 1.480 real sec...
[M::process] read 686076 sequences (100000276 bp) in 1.173 CPU sec, 1.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284632, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (311.71, 150.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688170 reads in 121.901 CPU sec, 3.885 real sec
[M::mem_process_seqs] Processed 686540 reads in 77.069 CPU sec, 2.456 real sec
sorting block 3, #reads = 1074191 mem = 536879047 peak = 884989952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284119, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 290, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (311.68, 150.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685490 sequences (100000076 bp) in 1.706 CPU sec, 1.803 real sec...
[M::process] read 690654 sequences (100000260 bp) in 1.170 CPU sec, 1.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284866, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (313.44, 151.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686088 reads in 121.095 CPU sec, 3.852 real sec
[M::mem_process_seqs] Processed 686076 reads in 76.089 CPU sec, 2.419 real sec
sorting block 4, #reads = 1072660 mem = 536878008 peak = 884989952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284468, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (316.17, 152.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690506 sequences (100000288 bp) in 1.637 CPU sec, 1.756 real sec...
[M::process] read 689308 sequences (100000211 bp) in 1.190 CPU sec, 1.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286550, 0, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 177, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430)
[M::mem_pestat] mean and std.dev: (164.44, 47.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 524)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (299.65, 147.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 280, 3806)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11058)
[M::mem_pestat] mean and std.dev: (1589.77, 1752.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14684)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685490 reads in 121.227 CPU sec, 3.861 real sec
sorting block 5, #reads = 1073529 mem = 536878701 peak = 884989952
[M::mem_process_seqs] Processed 690654 reads in 77.183 CPU sec, 2.449 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286198, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 280, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (301.98, 148.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 6, #reads = 1075722 mem = 536883390 peak = 884989952
[M::process] read 689492 sequences (100000225 bp) in 1.863 CPU sec, 1.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286278, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (304.80, 148.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (117, 167, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333)
[M::mem_pestat] mean and std.dev: (162.20, 54.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 405)
[M::mem_pestat] skip orientation RR
[M::process] read 688438 sequences (100000171 bp) in 1.220 CPU sec, 1.219 real sec...
[M::mem_process_seqs] Processed 690506 reads in 119.963 CPU sec, 3.813 real sec
[M::mem_process_seqs] Processed 689308 reads in 75.939 CPU sec, 2.412 real sec
sorting block 7, #reads = 1076145 mem = 536879876 peak = 884989952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285845, 0, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 291, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1554)
[M::mem_pestat] mean and std.dev: (334.15, 245.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2026)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (307.10, 151.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687898 sequences (100000082 bp) in 1.648 CPU sec, 1.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285138, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 686306 sequences (100000027 bp) in 1.250 CPU sec, 1.249 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (311.18, 153.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 159, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (172.33, 60.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689492 reads in 120.677 CPU sec, 3.840 real sec
[M::mem_process_seqs] Processed 688438 reads in 76.615 CPU sec, 2.432 real sec
sorting block 8, #reads = 1076609 mem = 536880211 peak = 884989952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285217, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (312.12, 152.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686738 sequences (100000091 bp) in 1.947 CPU sec, 2.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284812, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (316.21, 153.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686604 sequences (100000164 bp) in 1.280 CPU sec, 1.281 real sec...
[M::mem_process_seqs] Processed 687898 reads in 122.102 CPU sec, 3.880 real sec
[M::mem_process_seqs] Processed 686306 reads in 76.998 CPU sec, 2.441 real sec
sorting block 9, #reads = 1075192 mem = 536878917 peak = 884989952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284906, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (311.70, 151.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686524 sequences (100000255 bp) in 1.818 CPU sec, 1.946 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284956, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (313.19, 153.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686738 reads in 122.141 CPU sec, 3.889 real sec
[M::process] read 690902 sequences (100000192 bp) in 1.296 CPU sec, 1.296 real sec...
sorting block 10, #reads = 1074152 mem = 536880129 peak = 884989952
[M::mem_process_seqs] Processed 686604 reads in 77.841 CPU sec, 2.461 real sec
sorting block 11, #reads = 1073843 mem = 536880085 peak = 951734272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285206, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 884, 884)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2416)
[M::mem_pestat] mean and std.dev: (511.10, 375.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3182)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (314.46, 153.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 144, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477)
[M::mem_pestat] mean and std.dev: (164.92, 57.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 595)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689800 sequences (100000133 bp) in 2.029 CPU sec, 2.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286624, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 278, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (300.49, 148.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688946 sequences (100000070 bp) in 1.290 CPU sec, 1.288 real sec...
[M::mem_process_seqs] Processed 686524 reads in 122.637 CPU sec, 3.893 real sec
[M::mem_process_seqs] Processed 690902 reads in 77.943 CPU sec, 2.469 real sec
sorting block 12, #reads = 1072933 mem = 536880051 peak = 951734272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286346, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 282, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (303.46, 148.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688806 sequences (100000242 bp) in 1.786 CPU sec, 1.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285825, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (306.33, 148.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 153, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 307)
[M::mem_pestat] mean and std.dev: (155.36, 49.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 366)
[M::mem_pestat] skip orientation RR
[M::process] read 688070 sequences (100000070 bp) in 1.310 CPU sec, 1.311 real sec...
[M::mem_process_seqs] Processed 689800 reads in 121.868 CPU sec, 3.878 real sec
[M::mem_process_seqs] Processed 688946 reads in 77.715 CPU sec, 2.462 real sec
sorting block 13, #reads = 1076830 mem = 536880892 peak = 951734272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285472, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 215, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505)
[M::mem_pestat] mean and std.dev: (171.33, 56.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 626)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (308.55, 151.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686662 sequences (100000214 bp) in 2.189 CPU sec, 2.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285110, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 292, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (313.65, 153.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (84, 185, 254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (155.44, 67.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 764)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688806 reads in 123.135 CPU sec, 3.914 real sec
[M::process] read 685944 sequences (100000294 bp) in 1.351 CPU sec, 1.366 real sec...
sorting block 14, #reads = 1074624 mem = 536877975 peak = 951734272
[M::mem_process_seqs] Processed 688070 reads in 78.732 CPU sec, 2.487 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285101, 4, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 297, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (317.85, 153.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 305, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513)
[M::mem_pestat] mean and std.dev: (243.33, 68.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 617)
[M::mem_pestat] skip orientation RR
sorting block 15, #reads = 1075044 mem = 536883907 peak = 951734272
[M::process] read 686056 sequences (100000124 bp) in 2.091 CPU sec, 2.255 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284706, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (319.68, 153.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 344, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (273.17, 97.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686662 reads in 121.683 CPU sec, 3.875 real sec
[M::mem_process_seqs] Processed 685944 reads in 77.470 CPU sec, 2.445 real sec
[M::process] read 686560 sequences (100000215 bp) in 1.528 CPU sec, 1.571 real sec...
sorting block 16, #reads = 1074408 mem = 536882112 peak = 951734272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284891, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (317.15, 154.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686124 sequences (100000072 bp) in 1.982 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284210, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 228, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (195.92, 79.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (313.82, 152.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686056 reads in 121.342 CPU sec, 3.861 real sec
[M::process] read 689818 sequences (100000223 bp) in 1.361 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 686560 reads in 77.666 CPU sec, 2.456 real sec
sorting block 17, #reads = 1073090 mem = 536881910 peak = 951734272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284857, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (316.76, 154.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689788 sequences (100000245 bp) in 1.961 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285829, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (300.92, 146.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686124 reads in 124.108 CPU sec, 3.953 real sec
[M::process] read 688980 sequences (100000222 bp) in 1.410 CPU sec, 1.418 real sec...
[M::mem_process_seqs] Processed 689818 reads in 79.910 CPU sec, 2.526 real sec
sorting block 18, #reads = 1073277 mem = 536878141 peak = 951734272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286213, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 134, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452)
[M::mem_pestat] mean and std.dev: (164.45, 59.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (299.67, 144.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688340 sequences (100000010 bp) in 2.222 CPU sec, 2.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285638, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (303.18, 145.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689788 reads in 125.778 CPU sec, 4.006 real sec
sorting block 19, #reads = 1075420 mem = 536878370 peak = 951734272
[M::process] read 688238 sequences (100000172 bp) in 1.476 CPU sec, 1.501 real sec...
[M::mem_process_seqs] Processed 688980 reads in 79.917 CPU sec, 2.522 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285831, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (307.65, 148.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 207, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (272.50, 177.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1333)
[M::mem_pestat] skip orientation RR
sorting block 20, #reads = 1075910 mem = 536880611 peak = 1004642304
[M::process] read 687374 sequences (100000233 bp) in 2.201 CPU sec, 2.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284850, 2, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 291, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (286.90, 97.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (311.89, 152.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 212, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457)
[M::mem_pestat] mean and std.dev: (195.00, 72.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 561)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688340 reads in 126.908 CPU sec, 4.062 real sec
[M::mem_process_seqs] Processed 688238 reads in 81.967 CPU sec, 2.594 real sec
[M::process] read 686316 sequences (100000170 bp) in 2.071 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285003, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (315.03, 153.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 145, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3141)
[M::mem_pestat] mean and std.dev: (617.36, 820.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4151)
[M::mem_pestat] skip orientation RR
sorting block 21, #reads = 1076190 mem = 536880707 peak = 1004642304
[M::process] read 686016 sequences (100000109 bp) in 2.361 CPU sec, 2.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284961, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (318.52, 153.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687374 reads in 128.872 CPU sec, 4.121 real sec
[M::mem_process_seqs] Processed 686316 reads in 82.223 CPU sec, 2.607 real sec
sorting block 22, #reads = 1074343 mem = 536880735 peak = 1004642304
[M::process] read 685836 sequences (100000291 bp) in 2.199 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284284, 0, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 248, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (236.22, 105.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (318.78, 154.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686224 sequences (100000054 bp) in 2.197 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284589, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 204, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404)
[M::mem_pestat] mean and std.dev: (225.60, 92.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 595)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 295, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (316.60, 152.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686016 reads in 131.235 CPU sec, 4.205 real sec
[M::mem_process_seqs] Processed 685836 reads in 83.816 CPU sec, 2.657 real sec
sorting block 23, #reads = 1073137 mem = 536879157 peak = 1004642304
[M::process] read 690304 sequences (100000125 bp) in 2.312 CPU sec, 2.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284604, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (313.85, 152.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1073707 mem = 536880124 peak = 1004642304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286444, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (300.21, 148.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 692056 sequences (100000016 bp) in 2.189 CPU sec, 2.301 real sec...
[M::mem_process_seqs] Processed 686224 reads in 134.517 CPU sec, 4.306 real sec
[M::mem_process_seqs] Processed 690304 reads in 86.866 CPU sec, 2.751 real sec
[M::process] read 691246 sequences (100000241 bp) in 2.324 CPU sec, 2.469 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287001, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 271, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (294.43, 145.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1072729 mem = 536879253 peak = 1004642304
[M::process] read 690232 sequences (100000072 bp) in 2.115 CPU sec, 2.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286556, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 275, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (299.19, 148.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 692056 reads in 136.134 CPU sec, 4.357 real sec
[M::mem_process_seqs] Processed 691246 reads in 86.545 CPU sec, 2.737 real sec
sorting block 26, #reads = 1075006 mem = 536877956 peak = 1004642304
[M::process] read 689694 sequences (100000053 bp) in 2.240 CPU sec, 2.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286346, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 279, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (302.60, 149.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689530 sequences (100000247 bp) in 2.221 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286750, 0, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 281, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (303.31, 149.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 146, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (173.00, 99.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 745)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690232 reads in 136.834 CPU sec, 4.377 real sec
[M::mem_process_seqs] Processed 689694 reads in 86.558 CPU sec, 2.741 real sec
sorting block 27, #reads = 1074034 mem = 536880189 peak = 1004642304
[M::process] read 688106 sequences (100000142 bp) in 2.239 CPU sec, 2.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286003, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (303.05, 148.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 189, 219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415)
[M::mem_pestat] mean and std.dev: (161.10, 49.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] skip orientation RR
[M::process] read 687064 sequences (100000164 bp) in 2.143 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285519, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 284, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (306.35, 148.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689530 reads in 137.734 CPU sec, 4.400 real sec
sorting block 28, #reads = 1073674 mem = 536881083 peak = 1004642304
[M::mem_process_seqs] Processed 688106 reads in 87.293 CPU sec, 2.767 real sec
[M::process] read 686750 sequences (100000019 bp) in 2.136 CPU sec, 2.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285207, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 184, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365)
[M::mem_pestat] mean and std.dev: (166.70, 52.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 447)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (308.66, 147.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 29, #reads = 1072167 mem = 536877071 peak = 1004642304
[M::process] read 686352 sequences (100000081 bp) in 2.227 CPU sec, 2.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285222, 1, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 172, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158)
[M::mem_pestat] mean and std.dev: (243.70, 154.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1502)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (308.55, 147.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 687064 reads in 140.241 CPU sec, 4.476 real sec
[M::mem_process_seqs] Processed 686750 reads in 88.842 CPU sec, 2.809 real sec
[M::process] read 693430 sequences (100000245 bp) in 2.044 CPU sec, 2.137 real sec...
sorting block 30, #reads = 1071144 mem = 536882645 peak = 1004642304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284906, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (308.42, 146.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 693000 sequences (100000186 bp) in 2.079 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287978, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 264, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (288.25, 142.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686352 reads in 140.977 CPU sec, 4.500 real sec
[M::mem_process_seqs] Processed 693430 reads in 89.497 CPU sec, 2.823 real sec
[M::process] read 691506 sequences (100000084 bp) in 1.802 CPU sec, 1.869 real sec...
sorting block 31, #reads = 1070602 mem = 536879073 peak = 1004642304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 288002, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 268, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (291.35, 144.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690374 sequences (100000237 bp) in 2.457 CPU sec, 2.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287733, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 273, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (294.79, 143.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 693000 reads in 137.390 CPU sec, 4.378 real sec
[M::mem_process_seqs] Processed 691506 reads in 85.518 CPU sec, 2.702 real sec
sorting block 32, #reads = 1073690 mem = 536878760 peak = 1004642304
[M::process] read 690304 sequences (100000244 bp) in 1.826 CPU sec, 1.897 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286853, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (297.59, 145.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 304, 821)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2137)
[M::mem_pestat] mean and std.dev: (458.15, 406.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2795)
[M::mem_pestat] skip orientation RR
sorting block 33, #reads = 1075535 mem = 536882931 peak = 1004642304
[M::process] read 689288 sequences (100000117 bp) in 2.015 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286686, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 279, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (299.55, 145.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690374 reads in 134.225 CPU sec, 4.285 real sec
[M::mem_process_seqs] Processed 690304 reads in 85.083 CPU sec, 2.684 real sec
[M::process] read 687582 sequences (100000045 bp) in 1.757 CPU sec, 1.797 real sec...
sorting block 34, #reads = 1073471 mem = 536877595 peak = 1004642304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286666, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (302.21, 147.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 166, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348)
[M::mem_pestat] mean and std.dev: (158.42, 43.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 423)
[M::mem_pestat] skip orientation RR
[M::process] read 687320 sequences (100000197 bp) in 2.416 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285681, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 168, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (202.08, 96.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.09, 148.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 689288 reads in 135.308 CPU sec, 4.308 real sec
[M::mem_process_seqs] Processed 687582 reads in 85.621 CPU sec, 2.705 real sec
[M::process] read 686884 sequences (100000084 bp) in 1.751 CPU sec, 1.817 real sec...
sorting block 35, #reads = 1073729 mem = 536878471 peak = 1004642304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285385, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (309.59, 149.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 243, 730)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1880)
[M::mem_pestat] mean and std.dev: (259.45, 176.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2455)
[M::mem_pestat] skip orientation RR
[M::process] read 689176 sequences (100000248 bp) in 2.051 CPU sec, 2.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285410, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 289, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (310.27, 149.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687320 reads in 137.981 CPU sec, 4.402 real sec
[M::process] read 692834 sequences (100000275 bp) in 1.522 CPU sec, 1.542 real sec...
[M::mem_process_seqs] Processed 686884 reads in 87.331 CPU sec, 2.757 real sec
sorting block 36, #reads = 1071578 mem = 536876278 peak = 1004642304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286146, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (302.86, 148.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 160, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (204.50, 113.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RR
[M::process] read 691064 sequences (100000281 bp) in 2.159 CPU sec, 2.282 real sec...
[M::process] read 690560 sequences (100000211 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287603, 0, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 217, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (248.90, 151.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 269, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (290.90, 143.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 250, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (221.00, 90.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689176 reads in 141.403 CPU sec, 4.501 real sec
sorting block 37, #reads = 1071876 mem = 536877848 peak = 1004642304
[M::mem_process_seqs] Processed 692834 reads in 85.785 CPU sec, 2.721 real sec
sorting block 38, #reads = 1072445 mem = 536878189 peak = 1069666304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287019, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 275, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (295.38, 143.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689866 sequences (100000281 bp) in 2.133 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287618, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 277, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (297.13, 143.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691064 reads in 134.314 CPU sec, 4.276 real sec
[M::process] read 688976 sequences (100000072 bp) in 1.543 CPU sec, 1.544 real sec...
[M::mem_process_seqs] Processed 690560 reads in 84.105 CPU sec, 2.662 real sec
sorting block 39, #reads = 1076237 mem = 536879805 peak = 1069666304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287019, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (300.40, 145.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688476 sequences (100000267 bp) in 2.492 CPU sec, 2.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286316, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (301.55, 144.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689866 reads in 134.897 CPU sec, 4.293 real sec
[M::mem_process_seqs] Processed 688976 reads in 84.947 CPU sec, 2.679 real sec
[M::process] read 688128 sequences (100000117 bp) in 1.752 CPU sec, 1.804 real sec...
sorting block 40, #reads = 1074681 mem = 536878834 peak = 1069666304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285881, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (302.07, 143.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687770 sequences (100000226 bp) in 2.267 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285887, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (305.09, 145.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (124, 163, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 319)
[M::mem_pestat] mean and std.dev: (143.62, 34.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 384)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688476 reads in 137.082 CPU sec, 4.378 real sec
[M::mem_process_seqs] Processed 688128 reads in 86.396 CPU sec, 2.732 real sec
sorting block 41, #reads = 1073738 mem = 536880104 peak = 1069666304
[M::process] read 687774 sequences (100000224 bp) in 1.984 CPU sec, 2.061 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285957, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (307.89, 147.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1071879 mem = 536878217 peak = 1069666304
[M::process] read 691080 sequences (100000215 bp) in 2.099 CPU sec, 2.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285126, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 287, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (307.77, 148.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687770 reads in 140.238 CPU sec, 4.475 real sec
[M::mem_process_seqs] Processed 687774 reads in 88.535 CPU sec, 2.793 real sec
[M::process] read 693420 sequences (100000221 bp) in 1.845 CPU sec, 1.915 real sec...
sorting block 43, #reads = 1071367 mem = 536887588 peak = 1069666304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286453, 0, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 225, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (232.29, 98.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 273, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (296.76, 145.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 208, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (193.18, 75.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 691680 sequences (100000265 bp) in 2.352 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287937, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 266, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (289.50, 142.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691080 reads in 143.396 CPU sec, 4.578 real sec
[M::mem_process_seqs] Processed 693420 reads in 89.702 CPU sec, 2.832 real sec
[M::process] read 690850 sequences (100000258 bp) in 2.223 CPU sec, 2.330 real sec...
sorting block 44, #reads = 1069824 mem = 536879196 peak = 1069666304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286920, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 274, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (295.46, 144.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690076 sequences (100000000 bp) in 2.340 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286987, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 362, 837)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2021)
[M::mem_pestat] mean and std.dev: (437.91, 281.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2613)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (298.77, 145.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 691680 reads in 139.957 CPU sec, 4.481 real sec
[M::mem_process_seqs] Processed 690850 reads in 88.849 CPU sec, 2.821 real sec
sorting block 45, #reads = 1074302 mem = 536878641 peak = 1069666304
[M::process] read 689310 sequences (100000295 bp) in 1.751 CPU sec, 1.806 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287064, 0, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (301.68, 146.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 232, 883)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2365)
[M::mem_pestat] mean and std.dev: (475.29, 442.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3106)
[M::mem_pestat] skip orientation RR
sorting block 46, #reads = 1073775 mem = 536878972 peak = 1069666304
[M::process] read 688930 sequences (100000189 bp) in 2.161 CPU sec, 2.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286272, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 283, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (303.87, 147.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690076 reads in 137.887 CPU sec, 4.404 real sec
[M::mem_process_seqs] Processed 689310 reads in 87.345 CPU sec, 2.758 real sec
[M::process] read 688094 sequences (100000006 bp) in 1.759 CPU sec, 1.818 real sec...
sorting block 47, #reads = 1073456 mem = 536878858 peak = 1069666304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285964, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (302.19, 145.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687468 sequences (100000143 bp) in 2.307 CPU sec, 2.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285625, 0, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 186, 1072)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2926)
[M::mem_pestat] mean and std.dev: (251.45, 263.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3853)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 286, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (304.12, 144.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 221, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 373)
[M::mem_pestat] mean and std.dev: (198.50, 42.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 445)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688930 reads in 138.265 CPU sec, 4.400 real sec
[M::process] read 686896 sequences (100000065 bp) in 1.481 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 688094 reads in 86.233 CPU sec, 2.725 real sec
sorting block 48, #reads = 1072606 mem = 536881276 peak = 1069666304
[M::process] read 53474 sequences (7786167 bp) in 0.297 CPU sec, 0.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285741, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (306.37, 145.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285232, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (307.62, 146.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 186, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (192.70, 104.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 22165, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (307.94, 146.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687468 reads in 139.164 CPU sec, 4.526 real sec
[M::mem_process_seqs] Processed 686896 reads in 89.884 CPU sec, 2.913 real sec
[M::mem_process_seqs] Processed 53474 reads in 90.887 CPU sec, 2.950 real sec
sorting block 49, #reads = 1072645 mem = 536879370 peak = 1070170112
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 54462986
overall: 69840465920 mem 6952.657 user 279.949 sys 843.974 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0002_B23KGCJLT4_1.L008\tPU:L008\tSM:NA12878_c_0002_gDNA_0002_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0002_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 844.013 sec; CPU: 7232.608 sec
sorting block 50, #reads = 1037567 mem = 520460019 peak = 1193930752
uniq header count 1
merging 51 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2823862808
output reads: 54733032
bam_mem_sort: 51 calls 19.681 user 0.202 sys 19.931 real
bam_write: 51 calls 19.788 user 0.636 sys 20.823 real
execute: 1 calls 12.838 user 0.638 sys 860.414 real
merge_files: 1 calls 12.817 user 0.623 sys 15.553 real
parse_chunk: 816 calls 27.573 user 6.505 sys 34.764 real
read_chunk: 2205 calls 21.091 user 20.341 sys 188.600 real
sort_block: 1 calls 17.799 user 19.829 sys 183.198 real
write_chunk: 1387 calls 12.491 user 0.466 sys 14.961 real
overall: 2525716480 mem 596.069 user 33.319 sys 860.440 real
ls: cannot access '*.cram': No such file or directory