.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1029_01S-N1-BDNA-1_B23KGCJLT4_1;L004;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/aa/d33e77fc295f0e482cff7e4f2d99e7/.command.err
Size: 71.6 KB
Task: DAQ:SENTIEON_BWAMEM (1029_01S-N1-BDNA-1_B23KGCJLT4_1;L004;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_01S-N1-BDNA-1_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1673.858 CPU sec, 357.863 real sec
[M::process] read 680702 sequences (100000229 bp) in 0.935 CPU sec, 0.941 real sec...
[M::process] read 679766 sequences (100000285 bp) in 1.170 CPU sec, 1.167 real sec...
[M::process] read 679036 sequences (100000265 bp) in 1.130 CPU sec, 1.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282837, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 338, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (357.50, 172.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677954 sequences (100000223 bp) in 1.450 CPU sec, 1.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281363, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 353, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (370.28, 176.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281966, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 346, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (364.10, 173.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680702 reads in 206.709 CPU sec, 6.592 real sec
[M::mem_process_seqs] Processed 679036 reads in 136.283 CPU sec, 4.386 real sec
[M::mem_process_seqs] Processed 679766 reads in 147.618 CPU sec, 4.761 real sec
sorting block 0, #reads = 1081286 mem = 536886187 peak = 608727040
[M::process] read 677912 sequences (100000192 bp) in 1.706 CPU sec, 1.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281447, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 358, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (374.98, 177.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679294 sequences (100000252 bp) in 1.268 CPU sec, 1.290 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280557, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 360, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (377.09, 178.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679654 sequences (100000295 bp) in 1.150 CPU sec, 1.145 real sec...
[M::mem_process_seqs] Processed 677954 reads in 128.936 CPU sec, 4.088 real sec
sorting block 1, #reads = 1080031 mem = 536878706 peak = 794324992
[M::mem_process_seqs] Processed 677912 reads in 80.821 CPU sec, 2.546 real sec
sorting block 2, #reads = 1078878 mem = 536877510 peak = 892321792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281625, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 349, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (367.24, 175.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678416 sequences (100000021 bp) in 1.846 CPU sec, 1.995 real sec...
[M::process] read 678142 sequences (100000264 bp) in 1.413 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281786, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (363.47, 175.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679294 reads in 128.426 CPU sec, 4.069 real sec
[M::mem_process_seqs] Processed 679654 reads in 80.580 CPU sec, 2.550 real sec
sorting block 3, #reads = 1080545 mem = 536878756 peak = 892321792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281417, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 351, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (370.34, 176.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677930 sequences (100000046 bp) in 1.931 CPU sec, 2.063 real sec...
[M::process] read 677612 sequences (100000274 bp) in 1.240 CPU sec, 1.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281051, 3, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (986, 1654, 1654)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2990)
[M::mem_pestat] mean and std.dev: (1320.90, 551.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3658)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 352, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (371.38, 177.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 678416 reads in 129.608 CPU sec, 4.121 real sec
[M::mem_process_seqs] Processed 678142 reads in 81.342 CPU sec, 2.577 real sec
sorting block 4, #reads = 1080007 mem = 536879328 peak = 892321792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280704, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 354, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (373.04, 177.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680896 sequences (100000172 bp) in 1.859 CPU sec, 2.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280839, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 359, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (378.13, 180.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679650 sequences (100000250 bp) in 1.300 CPU sec, 1.301 real sec...
[M::mem_process_seqs] Processed 677930 reads in 129.008 CPU sec, 4.106 real sec
sorting block 5, #reads = 1078896 mem = 536877716 peak = 892321792
[M::mem_process_seqs] Processed 677612 reads in 81.229 CPU sec, 2.575 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281968, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 340, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (358.01, 172.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679660 sequences (100000038 bp) in 2.562 CPU sec, 2.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281431, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 349, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (365.14, 173.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680896 reads in 131.369 CPU sec, 4.168 real sec
sorting block 6, #reads = 1079600 mem = 536879068 peak = 892321792
[M::process] read 678388 sequences (100000128 bp) in 1.460 CPU sec, 1.477 real sec...
[M::mem_process_seqs] Processed 679650 reads in 82.686 CPU sec, 2.604 real sec
sorting block 7, #reads = 1080648 mem = 536880036 peak = 934162432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281493, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 350, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (365.39, 172.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677782 sequences (100000131 bp) in 1.890 CPU sec, 2.009 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281780, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 360, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (374.08, 175.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679200 sequences (100000242 bp) in 1.420 CPU sec, 1.422 real sec...
[M::mem_process_seqs] Processed 679660 reads in 131.097 CPU sec, 4.173 real sec
[M::mem_process_seqs] Processed 678388 reads in 82.248 CPU sec, 2.607 real sec
sorting block 8, #reads = 1079388 mem = 536886310 peak = 934162432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281289, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 364, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (379.09, 177.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679888 sequences (100000139 bp) in 2.177 CPU sec, 2.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282048, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 353, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (370.12, 176.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677880 sequences (100000143 bp) in 1.340 CPU sec, 1.346 real sec...
[M::mem_process_seqs] Processed 677782 reads in 130.916 CPU sec, 4.162 real sec
[M::mem_process_seqs] Processed 679200 reads in 82.596 CPU sec, 2.614 real sec
sorting block 9, #reads = 1079446 mem = 536880792 peak = 934162432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281761, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 347, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (365.00, 175.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678022 sequences (100000069 bp) in 1.978 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280796, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 363, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (376.93, 175.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677862 sequences (100000265 bp) in 1.540 CPU sec, 1.548 real sec...
[M::mem_process_seqs] Processed 679888 reads in 131.880 CPU sec, 4.189 real sec
sorting block 10, #reads = 1080455 mem = 536877546 peak = 934162432
[M::mem_process_seqs] Processed 677880 reads in 83.151 CPU sec, 2.622 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280554, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 359, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (375.73, 178.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677590 sequences (100000060 bp) in 2.417 CPU sec, 2.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281369, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 356, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (373.91, 177.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678022 reads in 132.222 CPU sec, 4.200 real sec
sorting block 11, #reads = 1080005 mem = 536878202 peak = 934162432
[M::process] read 681326 sequences (100000014 bp) in 1.538 CPU sec, 1.555 real sec...
[M::mem_process_seqs] Processed 677862 reads in 83.814 CPU sec, 2.644 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281050, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 359, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (377.73, 179.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 12, #reads = 1079154 mem = 536878725 peak = 969687040
[M::process] read 680518 sequences (100000118 bp) in 2.256 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282390, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 337, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (354.61, 170.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677590 reads in 131.764 CPU sec, 4.207 real sec
[M::mem_process_seqs] Processed 681326 reads in 83.726 CPU sec, 2.644 real sec
[M::process] read 679796 sequences (100000076 bp) in 1.782 CPU sec, 1.865 real sec...
sorting block 13, #reads = 1078643 mem = 536878657 peak = 969687040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282239, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 344, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (359.82, 171.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678640 sequences (100000063 bp) in 2.199 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281469, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 350, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (364.57, 171.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680518 reads in 132.564 CPU sec, 4.226 real sec
[M::mem_process_seqs] Processed 679796 reads in 85.163 CPU sec, 2.682 real sec
[M::process] read 677776 sequences (100000139 bp) in 1.787 CPU sec, 1.847 real sec...
sorting block 14, #reads = 1081568 mem = 536877701 peak = 969687040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281344, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 360, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (372.62, 173.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678634 sequences (100000049 bp) in 2.046 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281574, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 366, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (378.54, 175.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678640 reads in 131.521 CPU sec, 4.192 real sec
[M::mem_process_seqs] Processed 677776 reads in 82.464 CPU sec, 2.599 real sec
[M::process] read 678526 sequences (100000160 bp) in 1.615 CPU sec, 1.652 real sec...
sorting block 15, #reads = 1080139 mem = 536880666 peak = 969687040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281513, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 356, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (370.32, 173.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1078934 mem = 536880431 peak = 969687040
[M::process] read 677896 sequences (100000296 bp) in 2.855 CPU sec, 3.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281260, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 360, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (373.61, 174.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678634 reads in 134.083 CPU sec, 4.272 real sec
[M::mem_process_seqs] Processed 678526 reads in 85.896 CPU sec, 2.713 real sec
[M::process] read 677398 sequences (100000107 bp) in 2.046 CPU sec, 2.167 real sec...
sorting block 17, #reads = 1078632 mem = 536879781 peak = 969687040
[M::process] read 677664 sequences (100000169 bp) in 1.828 CPU sec, 1.959 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280830, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 364, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (378.39, 175.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677398 reads in 71.451 CPU sec, 2.254 real sec
[M::process] read 677632 sequences (100000281 bp) in 1.513 CPU sec, 1.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281323, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 364, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (377.06, 175.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281069, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 360, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (376.15, 177.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677896 reads in 198.828 CPU sec, 6.319 real sec
sorting block 18, #reads = 1078737 mem = 536881662 peak = 969687040
[M::mem_process_seqs] Processed 677664 reads in 93.982 CPU sec, 2.965 real sec
[M::process] read 681382 sequences (100000082 bp) in 2.386 CPU sec, 2.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280875, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 361, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (379.39, 180.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1079178 mem = 536878856 peak = 969687040
[M::process] read 680022 sequences (100000048 bp) in 2.219 CPU sec, 2.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281787, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (352.98, 169.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677632 reads in 128.767 CPU sec, 4.107 real sec
[M::mem_process_seqs] Processed 681382 reads in 83.067 CPU sec, 2.623 real sec
[M::process] read 679684 sequences (100000116 bp) in 1.953 CPU sec, 2.074 real sec...
sorting block 20, #reads = 1079301 mem = 536879223 peak = 969687040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281137, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (360.24, 170.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678232 sequences (100000283 bp) in 2.369 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281872, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (363.80, 170.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680022 reads in 128.376 CPU sec, 4.090 real sec
[M::mem_process_seqs] Processed 679684 reads in 82.593 CPU sec, 2.609 real sec
sorting block 21, #reads = 1080302 mem = 536878956 peak = 969687040
[M::process] read 677680 sequences (100000144 bp) in 2.117 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280882, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 359, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (372.81, 173.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679248 sequences (100000035 bp) in 1.986 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280788, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 363, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (377.11, 175.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678232 reads in 128.380 CPU sec, 4.096 real sec
[M::mem_process_seqs] Processed 677680 reads in 82.170 CPU sec, 2.595 real sec
sorting block 22, #reads = 1079684 mem = 536878097 peak = 969687040
[M::process] read 678728 sequences (100000080 bp) in 2.040 CPU sec, 2.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282208, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 351, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (366.72, 172.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678150 sequences (100000053 bp) in 2.126 CPU sec, 2.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280972, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 354, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (369.08, 172.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679248 reads in 128.784 CPU sec, 4.104 real sec
sorting block 23, #reads = 1079229 mem = 536880733 peak = 969687040
[M::mem_process_seqs] Processed 678728 reads in 82.082 CPU sec, 2.590 real sec
[M::process] read 678178 sequences (100000293 bp) in 1.919 CPU sec, 2.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280759, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 358, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (372.64, 173.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1079554 mem = 536879193 peak = 969687040
[M::process] read 678000 sequences (100000019 bp) in 2.166 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281005, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 358, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (373.76, 174.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678150 reads in 130.059 CPU sec, 4.146 real sec
[M::mem_process_seqs] Processed 678178 reads in 83.383 CPU sec, 2.628 real sec
[M::process] read 677748 sequences (100000231 bp) in 1.942 CPU sec, 2.059 real sec...
sorting block 25, #reads = 1078931 mem = 536879643 peak = 969687040
[M::process] read 682622 sequences (100000022 bp) in 2.134 CPU sec, 2.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280725, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 357, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (375.06, 177.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677748 reads in 71.836 CPU sec, 2.263 real sec
[M::process] read 680772 sequences (100000259 bp) in 1.664 CPU sec, 1.734 real sec...
sorting block 26, #reads = 1077751 mem = 536880260 peak = 969687040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280941, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 357, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (374.60, 176.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283153, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 327, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (351.95, 175.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678000 reads in 203.253 CPU sec, 6.444 real sec
[M::mem_process_seqs] Processed 682622 reads in 98.616 CPU sec, 3.107 real sec
[M::process] read 679458 sequences (100000299 bp) in 2.109 CPU sec, 2.192 real sec...
sorting block 27, #reads = 1079608 mem = 536878974 peak = 969687040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281766, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 344, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (362.87, 175.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678626 sequences (100000090 bp) in 2.207 CPU sec, 2.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281782, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 353, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (369.90, 176.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680772 reads in 130.541 CPU sec, 4.149 real sec
[M::mem_process_seqs] Processed 679458 reads in 83.398 CPU sec, 2.623 real sec
[M::process] read 678900 sequences (100000066 bp) in 1.639 CPU sec, 1.689 real sec...
sorting block 28, #reads = 1079260 mem = 536881399 peak = 969687040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281160, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 356, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (372.41, 176.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680566 sequences (100000173 bp) in 1.832 CPU sec, 1.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281508, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 350, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (366.66, 172.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680112 sequences (100000267 bp) in 1.400 CPU sec, 1.403 real sec...
[M::mem_process_seqs] Processed 678626 reads in 133.443 CPU sec, 4.242 real sec
[M::mem_process_seqs] Processed 678900 reads in 84.648 CPU sec, 2.676 real sec
sorting block 29, #reads = 1078094 mem = 536881356 peak = 969687040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282049, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (351.77, 168.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678798 sequences (100000014 bp) in 2.265 CPU sec, 2.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282154, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (350.25, 166.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678410 sequences (100000265 bp) in 1.430 CPU sec, 1.428 real sec...
[M::mem_process_seqs] Processed 680566 reads in 130.900 CPU sec, 4.153 real sec
sorting block 30, #reads = 1076874 mem = 536879220 peak = 969687040
[M::mem_process_seqs] Processed 680112 reads in 83.344 CPU sec, 2.629 real sec
sorting block 31, #reads = 1079128 mem = 536882202 peak = 1070804992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281804, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 344, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (360.28, 168.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678380 sequences (100000076 bp) in 2.080 CPU sec, 2.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281498, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 345, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (361.45, 167.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680666 sequences (100000204 bp) in 1.470 CPU sec, 1.469 real sec...
[M::mem_process_seqs] Processed 678798 reads in 138.687 CPU sec, 4.410 real sec
[M::mem_process_seqs] Processed 678410 reads in 89.743 CPU sec, 2.839 real sec
sorting block 32, #reads = 1077668 mem = 536877816 peak = 1070804992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281439, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 345, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (362.86, 169.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681520 sequences (100000093 bp) in 2.361 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282270, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 336, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (357.17, 173.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680356 sequences (100000272 bp) in 1.500 CPU sec, 1.494 real sec...
[M::mem_process_seqs] Processed 678380 reads in 139.406 CPU sec, 4.431 real sec
[M::mem_process_seqs] Processed 680666 reads in 88.834 CPU sec, 2.805 real sec
sorting block 33, #reads = 1076501 mem = 536879570 peak = 1070804992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283365, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 335, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (354.07, 170.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679458 sequences (100000011 bp) in 2.111 CPU sec, 2.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282541, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 340, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (357.37, 169.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681520 reads in 136.342 CPU sec, 4.337 real sec
[M::process] read 678702 sequences (100000162 bp) in 1.480 CPU sec, 1.486 real sec...
sorting block 34, #reads = 1077442 mem = 536879265 peak = 1070804992
[M::mem_process_seqs] Processed 680356 reads in 86.019 CPU sec, 2.715 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282370, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 347, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (362.98, 171.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1079244 mem = 536878961 peak = 1070804992
[M::process] read 680140 sequences (100000210 bp) in 2.481 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282235, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 353, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (368.49, 172.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679458 reads in 133.579 CPU sec, 4.243 real sec
[M::process] read 680244 sequences (100000066 bp) in 1.625 CPU sec, 1.649 real sec...
[M::mem_process_seqs] Processed 678702 reads in 84.730 CPU sec, 2.668 real sec
sorting block 36, #reads = 1078147 mem = 536878031 peak = 1070804992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282144, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 339, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (355.62, 167.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678874 sequences (100000210 bp) in 1.991 CPU sec, 2.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281652, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (353.49, 167.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678230 sequences (100000070 bp) in 1.460 CPU sec, 1.468 real sec...
[M::mem_process_seqs] Processed 680140 reads in 135.992 CPU sec, 4.329 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281688, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 349, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (363.85, 169.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677946 sequences (100000266 bp) in 1.744 CPU sec, 1.748 real sec...
[M::mem_process_seqs] Processed 678874 reads in 77.466 CPU sec, 2.639 real sec
sorting block 37, #reads = 1076933 mem = 536880748 peak = 1070804992
[M::mem_process_seqs] Processed 680244 reads in 185.414 CPU sec, 6.072 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281104, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 351, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (366.23, 169.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1077290 mem = 536880972 peak = 1070804992
[M::process] read 678324 sequences (100000148 bp) in 2.116 CPU sec, 2.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281019, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 355, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (370.01, 172.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678230 reads in 144.582 CPU sec, 4.594 real sec
[M::mem_process_seqs] Processed 677946 reads in 85.177 CPU sec, 2.686 real sec
[M::process] read 682658 sequences (100000216 bp) in 1.867 CPU sec, 1.979 real sec...
sorting block 39, #reads = 1078012 mem = 536875584 peak = 1070804992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281642, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 352, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (370.34, 175.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680898 sequences (100000075 bp) in 2.200 CPU sec, 2.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283632, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 327, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (347.37, 169.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678324 reads in 142.084 CPU sec, 4.517 real sec
[M::process] read 679676 sequences (100000037 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_process_seqs] Processed 682658 reads in 87.616 CPU sec, 2.768 real sec
sorting block 40, #reads = 1075105 mem = 536880193 peak = 1070804992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282428, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 338, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (355.40, 169.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678682 sequences (100000255 bp) in 2.189 CPU sec, 2.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281898, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 345, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (360.56, 169.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680898 reads in 135.062 CPU sec, 4.303 real sec
[M::process] read 679222 sequences (100000061 bp) in 1.622 CPU sec, 1.659 real sec...
[M::mem_process_seqs] Processed 679676 reads in 84.567 CPU sec, 2.668 real sec
sorting block 41, #reads = 1078047 mem = 536878613 peak = 1070804992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280983, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 350, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (365.41, 169.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679932 sequences (100000076 bp) in 2.040 CPU sec, 2.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281637, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 346, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (360.29, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679348 sequences (100000104 bp) in 1.470 CPU sec, 1.468 real sec...
[M::mem_process_seqs] Processed 678682 reads in 137.009 CPU sec, 4.367 real sec
sorting block 42, #reads = 1078975 mem = 536879242 peak = 1070804992
[M::mem_process_seqs] Processed 679222 reads in 87.602 CPU sec, 2.771 real sec
sorting block 43, #reads = 1076754 mem = 536878763 peak = 1070804992
[M::process] read 679596 sequences (100000030 bp) in 2.159 CPU sec, 2.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281424, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 344, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (358.56, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678894 sequences (100000081 bp) in 1.490 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 679348 reads in 78.879 CPU sec, 2.484 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281693, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 339, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (355.32, 166.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281721, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 343, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (358.31, 166.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679932 reads in 208.991 CPU sec, 6.646 real sec
[M::process] read 679166 sequences (100000202 bp) in 1.510 CPU sec, 1.513 real sec...
sorting block 44, #reads = 1077647 mem = 536876862 peak = 1070804992
[M::mem_process_seqs] Processed 679596 reads in 97.361 CPU sec, 3.094 real sec
sorting block 45, #reads = 1078755 mem = 536876719 peak = 1096429568
[M::process] read 682644 sequences (100000204 bp) in 2.702 CPU sec, 2.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282074, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 347, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (363.71, 171.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679166 reads in 77.548 CPU sec, 2.439 real sec
[M::process] read 682022 sequences (100000020 bp) in 1.661 CPU sec, 1.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281955, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 347, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (360.62, 166.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282505, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 326, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (350.14, 173.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678894 reads in 217.899 CPU sec, 6.909 real sec
[M::process] read 680684 sequences (100000274 bp) in 1.512 CPU sec, 1.515 real sec...
sorting block 46, #reads = 1075126 mem = 536878915 peak = 1096429568
[M::mem_process_seqs] Processed 682644 reads in 106.347 CPU sec, 3.376 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282638, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 332, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (352.36, 171.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1075290 mem = 536879434 peak = 1096429568
[M::process] read 679356 sequences (100000151 bp) in 2.442 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282129, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 341, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (358.79, 171.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679196 sequences (100000051 bp) in 1.440 CPU sec, 1.435 real sec...
[M::mem_process_seqs] Processed 682022 reads in 136.122 CPU sec, 4.329 real sec
[M::mem_process_seqs] Processed 680684 reads in 85.295 CPU sec, 2.708 real sec
sorting block 48, #reads = 1079525 mem = 536879185 peak = 1096429568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281259, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 349, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (364.15, 170.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680798 sequences (100000282 bp) in 2.328 CPU sec, 2.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281963, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 353, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (367.13, 171.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679826 sequences (100000063 bp) in 1.420 CPU sec, 1.422 real sec...
[M::mem_process_seqs] Processed 679356 reads in 134.031 CPU sec, 4.252 real sec
[M::mem_process_seqs] Processed 679196 reads in 85.069 CPU sec, 2.688 real sec
sorting block 49, #reads = 1077269 mem = 536879322 peak = 1096429568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282105, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (349.75, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679330 sequences (100000034 bp) in 2.312 CPU sec, 2.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282438, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (353.28, 164.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679112 sequences (100000093 bp) in 1.440 CPU sec, 1.441 real sec...
[M::mem_process_seqs] Processed 680798 reads in 133.883 CPU sec, 4.247 real sec
[M::mem_process_seqs] Processed 679826 reads in 83.784 CPU sec, 2.649 real sec
sorting block 50, #reads = 1076876 mem = 536879675 peak = 1096429568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282216, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 344, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (359.43, 167.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678630 sequences (100000034 bp) in 2.213 CPU sec, 2.400 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281942, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 344, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (358.86, 166.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 565730 sequences (83413351 bp) in 1.300 CPU sec, 1.297 real sec...
[M::mem_process_seqs] Processed 679330 reads in 131.151 CPU sec, 4.168 real sec
sorting block 51, #reads = 1078609 mem = 536878374 peak = 1096429568
[M::mem_process_seqs] Processed 679112 reads in 82.641 CPU sec, 2.612 real sec
sorting block 52, #reads = 1077786 mem = 536879956 peak = 1166307328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281815, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 347, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (361.68, 167.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 234604, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 354, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (369.78, 173.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678630 reads in 117.249 CPU sec, 3.801 real sec
[M::mem_process_seqs] Processed 565730 reads in 69.650 CPU sec, 2.254 real sec
sorting block 53, #reads = 1078429 mem = 536880372 peak = 1166307328
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 58302598
overall: 69801754624 mem 7614.734 user 292.375 sys 564.852 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.1029_01S-N1-BDNA-1_B23KGCJLT4_1.L004\tPU:L004\tSM:1029_01S-N1-BDNA-1_B23KGCJLT4_1\tLB:1029_01S-N1-BDNA-1_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_01S-N1-BDNA-1_B23KGCJLT4_1_R1.fastq.gz 1029_01S-N1-BDNA-1_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 564.886 sec; CPU: 7907.112 sec
sorting block 54, #reads = 310380 mem = 154936902 peak = 1166307328
uniq header count 1
merging 55 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3129931433
output reads: 58557696
bam_mem_sort: 55 calls 24.142 user 0.238 sys 24.454 real
bam_write: 55 calls 11.621 user 0.565 sys 12.130 real
execute: 1 calls 10.741 user 0.830 sys 581.733 real
merge_files: 1 calls 10.723 user 0.803 sys 16.526 real
parse_chunk: 869 calls 34.603 user 8.819 sys 43.952 real
read_chunk: 2343 calls 18.037 user 18.504 sys 206.255 real
sort_block: 1 calls 14.100 user 17.990 sys 199.791 real
write_chunk: 1472 calls 10.448 user 0.621 sys 15.849 real
overall: 2574385152 mem 654.010 user 33.459 sys 581.754 real
ls: cannot access '*.cram': No such file or directory