.command.log - Workdir - DAQ:SENTIEON_BWAMEM (NA12878_c_0002_gDNA_0002_B23KGCJLT4_1;L002;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7d/3e392d30b04759d2c6df3dda6419d1/.command.log
Size: 79.1 KB
Task: DAQ:SENTIEON_BWAMEM (NA12878_c_0002_gDNA_0002_B23KGCJLT4_1;L002;0001)
Attempt
Content

  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7d/3e392d30b04759d2c6df3dda6419d1/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/03/cbaf00a4e7359338b7abff3a39104d/NA12878_c_0002_gDNA_0002_B23KGCJLT4_1/NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/7d/3e392d30b04759d2c6df3dda6419d1/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/03/cbaf00a4e7359338b7abff3a39104d/NA12878_c_0002_gDNA_0002_B23KGCJLT4_1/NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R2.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NA12878_c_0002_gDNA_0002_B23KGCJLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1936.930 CPU sec, 256.872 real sec
[M::process] read 688606 sequences (100000023 bp) in 0.930 CPU sec, 0.934 real sec...
[M::process] read 686850 sequences (100000202 bp) in 1.170 CPU sec, 1.167 real sec...
[M::process] read 686406 sequences (100000289 bp) in 1.179 CPU sec, 1.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286178, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 289, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (308.72, 149.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685596 sequences (100000098 bp) in 1.110 CPU sec, 1.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284736, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (318.02, 152.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 625, 3693)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10647)
[M::mem_pestat] mean and std.dev: (1335.18, 1463.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14124)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285233, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (312.96, 148.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (92, 125, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413)
[M::mem_pestat] mean and std.dev: (144.89, 73.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 520)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688606 reads in 204.718 CPU sec, 6.523 real sec
[M::mem_process_seqs] Processed 686406 reads in 131.501 CPU sec, 4.230 real sec
[M::mem_process_seqs] Processed 686850 reads in 142.170 CPU sec, 4.589 real sec
sorting block 0, #reads = 1075132 mem = 536876099 peak = 588267520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284571, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 302, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (320.29, 152.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686638 sequences (100000139 bp) in 1.924 CPU sec, 2.139 real sec...
[M::mem_process_seqs] Processed 685596 reads in 71.440 CPU sec, 2.380 real sec
sorting block 1, #reads = 1073740 mem = 536878258 peak = 770404352
[M::process] read 686764 sequences (100000135 bp) in 1.661 CPU sec, 1.792 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284613, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 293, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (312.90, 150.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686998 sequences (100000284 bp) in 1.140 CPU sec, 1.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284682, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (310.58, 148.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686910 sequences (100000250 bp) in 1.130 CPU sec, 1.128 real sec...
[M::mem_process_seqs] Processed 686638 reads in 124.266 CPU sec, 3.910 real sec
sorting block 2, #reads = 1073662 mem = 536879624 peak = 776048640
[M::process] read 686486 sequences (100000035 bp) in 1.302 CPU sec, 1.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285100, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.69, 148.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 374, 9797)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28925)
[M::mem_pestat] mean and std.dev: (2908.36, 4221.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 38489)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686998 reads in 72.032 CPU sec, 2.434 real sec
[M::mem_process_seqs] Processed 686764 reads in 165.723 CPU sec, 5.395 real sec
sorting block 3, #reads = 1073398 mem = 536879746 peak = 776048640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284879, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (310.12, 149.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685770 sequences (100000288 bp) in 1.950 CPU sec, 2.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285092, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (312.92, 151.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688454 sequences (100000229 bp) in 1.250 CPU sec, 1.247 real sec...
[M::mem_process_seqs] Processed 686910 reads in 136.468 CPU sec, 4.331 real sec
[M::mem_process_seqs] Processed 686486 reads in 78.876 CPU sec, 2.502 real sec
sorting block 4, #reads = 1074015 mem = 536880490 peak = 776048640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284549, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (316.31, 153.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686654 sequences (100000170 bp) in 1.859 CPU sec, 2.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285257, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 292, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (310.51, 149.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685972 sequences (100000189 bp) in 1.270 CPU sec, 1.266 real sec...
[M::mem_process_seqs] Processed 685770 reads in 125.044 CPU sec, 3.975 real sec
sorting block 5, #reads = 1072686 mem = 536879925 peak = 776048640
[M::mem_process_seqs] Processed 688454 reads in 78.266 CPU sec, 2.484 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284760, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (317.06, 151.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (106, 145, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481)
[M::mem_pestat] mean and std.dev: (161.50, 56.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 606)
[M::mem_pestat] skip orientation RR
sorting block 6, #reads = 1073175 mem = 536878458 peak = 820629504
[M::process] read 685616 sequences (100000069 bp) in 2.120 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284946, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 189, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (204.27, 82.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 304, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (321.81, 153.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686252 sequences (100000084 bp) in 1.320 CPU sec, 1.317 real sec...
[M::mem_process_seqs] Processed 686654 reads in 124.170 CPU sec, 3.940 real sec
[M::mem_process_seqs] Processed 685972 reads in 78.780 CPU sec, 2.491 real sec
sorting block 7, #reads = 1073869 mem = 536880272 peak = 820629504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284332, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 304, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (321.20, 152.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686444 sequences (100000227 bp) in 2.101 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284747, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (316.06, 151.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 217, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 418)
[M::mem_pestat] mean and std.dev: (218.83, 64.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 497)
[M::mem_pestat] skip orientation RR
[M::process] read 686888 sequences (100000245 bp) in 1.320 CPU sec, 1.323 real sec...
[M::mem_process_seqs] Processed 685616 reads in 125.375 CPU sec, 3.979 real sec
[M::mem_process_seqs] Processed 686252 reads in 80.241 CPU sec, 2.532 real sec
sorting block 8, #reads = 1072508 mem = 536878904 peak = 820629504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284963, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (313.52, 149.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 339, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373)
[M::mem_pestat] mean and std.dev: (392.55, 197.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1761)
[M::mem_pestat] skip orientation RR
[M::process] read 686942 sequences (100000087 bp) in 2.196 CPU sec, 2.351 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284643, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 290, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (311.00, 149.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686444 reads in 126.586 CPU sec, 4.021 real sec
[M::process] read 686744 sequences (100000168 bp) in 1.370 CPU sec, 1.368 real sec...
[M::mem_process_seqs] Processed 686888 reads in 79.852 CPU sec, 2.525 real sec
sorting block 9, #reads = 1073961 mem = 536878958 peak = 820629504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284853, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (310.58, 149.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685954 sequences (100000276 bp) in 2.262 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284775, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (313.47, 152.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686942 reads in 127.515 CPU sec, 4.046 real sec
[M::process] read 688456 sequences (100000076 bp) in 1.398 CPU sec, 1.405 real sec...
sorting block 10, #reads = 1073537 mem = 536879680 peak = 820629504
[M::mem_process_seqs] Processed 686744 reads in 80.617 CPU sec, 2.544 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284708, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (316.85, 154.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (89, 176, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (197.67, 117.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RR
sorting block 11, #reads = 1073547 mem = 536878806 peak = 946524160
[M::process] read 686962 sequences (100000242 bp) in 2.339 CPU sec, 2.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285518, 1, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (311.55, 150.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 368, 630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1494)
[M::mem_pestat] mean and std.dev: (371.13, 202.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1926)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685954 reads in 127.998 CPU sec, 4.066 real sec
[M::process] read 686244 sequences (100000195 bp) in 1.531 CPU sec, 1.558 real sec...
[M::mem_process_seqs] Processed 688456 reads in 80.268 CPU sec, 2.528 real sec
sorting block 12, #reads = 1073095 mem = 536879237 peak = 946524160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285301, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 298, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (317.58, 153.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686424 sequences (100000068 bp) in 2.399 CPU sec, 2.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284904, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 340, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596)
[M::mem_pestat] mean and std.dev: (258.30, 88.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 302, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (319.80, 152.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686962 reads in 126.505 CPU sec, 4.026 real sec
[M::mem_process_seqs] Processed 686244 reads in 80.312 CPU sec, 2.535 real sec
[M::process] read 687074 sequences (100000107 bp) in 1.705 CPU sec, 1.770 real sec...
sorting block 13, #reads = 1074268 mem = 536879041 peak = 946524160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284850, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 299, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (318.24, 152.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686234 sequences (100000101 bp) in 2.273 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285080, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 294, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (314.42, 151.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (122, 148, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383)
[M::mem_pestat] mean and std.dev: (145.20, 49.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 470)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686424 reads in 127.016 CPU sec, 4.050 real sec
[M::mem_process_seqs] Processed 687074 reads in 80.674 CPU sec, 2.542 real sec
sorting block 14, #reads = 1073488 mem = 536879793 peak = 946524160
[M::process] read 686348 sequences (100000033 bp) in 1.916 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285059, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (315.91, 151.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1073568 mem = 536884889 peak = 946524160
[M::process] read 686532 sequences (100000289 bp) in 2.231 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284770, 0, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 223, 955)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579)
[M::mem_pestat] mean and std.dev: (477.50, 391.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3391)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 292, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (313.39, 151.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 201, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 369)
[M::mem_pestat] mean and std.dev: (205.09, 44.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 431)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686234 reads in 128.812 CPU sec, 4.106 real sec
[M::mem_process_seqs] Processed 686348 reads in 82.270 CPU sec, 2.594 real sec
[M::process] read 686282 sequences (100000094 bp) in 1.887 CPU sec, 2.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285204, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (313.39, 151.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1073742 mem = 536878395 peak = 946524160
[M::process] read 686344 sequences (100000224 bp) in 2.179 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284832, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (315.48, 153.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686532 reads in 129.134 CPU sec, 4.117 real sec
[M::mem_process_seqs] Processed 686282 reads in 81.978 CPU sec, 2.586 real sec
[M::process] read 688666 sequences (100000109 bp) in 1.866 CPU sec, 1.967 real sec...
sorting block 17, #reads = 1073673 mem = 536879774 peak = 946524160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284876, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (315.48, 153.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686716 sequences (100000213 bp) in 2.173 CPU sec, 2.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285703, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 288, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (310.65, 153.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686344 reads in 130.191 CPU sec, 4.151 real sec
[M::mem_process_seqs] Processed 688666 reads in 80.989 CPU sec, 2.558 real sec
sorting block 18, #reads = 1072786 mem = 536880529 peak = 946524160
[M::process] read 686334 sequences (100000121 bp) in 1.778 CPU sec, 1.864 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284925, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 296, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (315.50, 151.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686610 sequences (100000223 bp) in 2.204 CPU sec, 2.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285270, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 298, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (316.33, 150.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1070)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686716 reads in 126.213 CPU sec, 4.025 real sec
sorting block 19, #reads = 1075406 mem = 536877868 peak = 946524160
[M::mem_process_seqs] Processed 686334 reads in 80.350 CPU sec, 2.538 real sec
[M::process] read 686300 sequences (100000138 bp) in 1.843 CPU sec, 1.943 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284906, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (313.95, 150.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1074715 mem = 536877831 peak = 946524160
[M::process] read 686298 sequences (100000269 bp) in 2.094 CPU sec, 2.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284866, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 231, 919)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2337)
[M::mem_pestat] mean and std.dev: (403.09, 319.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3046)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (314.43, 150.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686610 reads in 127.761 CPU sec, 4.072 real sec
[M::mem_process_seqs] Processed 686300 reads in 81.398 CPU sec, 2.571 real sec
[M::process] read 686064 sequences (100000188 bp) in 1.684 CPU sec, 1.740 real sec...
sorting block 21, #reads = 1073228 mem = 536883038 peak = 946524160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284415, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (313.53, 150.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686400 sequences (100000113 bp) in 2.254 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283949, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (109, 164, 168)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286)
[M::mem_pestat] mean and std.dev: (146.11, 55.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 366)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (312.05, 150.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686298 reads in 130.348 CPU sec, 4.154 real sec
[M::mem_process_seqs] Processed 686064 reads in 82.561 CPU sec, 2.606 real sec
[M::process] read 686266 sequences (100000096 bp) in 1.732 CPU sec, 1.791 real sec...
sorting block 22, #reads = 1073815 mem = 536877877 peak = 946524160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284656, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (312.02, 150.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686106 sequences (100000119 bp) in 2.266 CPU sec, 2.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285211, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (313.32, 151.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686400 reads in 130.141 CPU sec, 4.141 real sec
[M::mem_process_seqs] Processed 686266 reads in 82.089 CPU sec, 2.591 real sec
sorting block 23, #reads = 1072335 mem = 536881529 peak = 946524160
[M::process] read 690620 sequences (100000159 bp) in 1.622 CPU sec, 1.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284284, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (314.23, 151.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1072538 mem = 536880135 peak = 958705664
[M::process] read 688238 sequences (100000231 bp) in 2.284 CPU sec, 2.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287001, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 135, 236)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502)
[M::mem_pestat] mean and std.dev: (149.56, 57.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 635)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 281, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (305.05, 152.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 686106 reads in 129.841 CPU sec, 4.132 real sec
[M::process] read 686866 sequences (100000171 bp) in 1.460 CPU sec, 1.484 real sec...
[M::mem_process_seqs] Processed 690620 reads in 82.989 CPU sec, 2.665 real sec
sorting block 25, #reads = 1073825 mem = 536880699 peak = 958705664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285676, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 292, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (312.30, 151.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686748 sequences (100000265 bp) in 2.203 CPU sec, 2.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284774, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (314.29, 150.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (105, 149, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (191.33, 109.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 745)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688238 reads in 126.332 CPU sec, 4.022 real sec
[M::process] read 688068 sequences (100000226 bp) in 1.387 CPU sec, 1.402 real sec...
[M::mem_process_seqs] Processed 686866 reads in 80.237 CPU sec, 2.533 real sec
sorting block 26, #reads = 1076224 mem = 536880778 peak = 958705664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285683, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (313.88, 149.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687718 sequences (100000265 bp) in 2.369 CPU sec, 2.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285612, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (305.09, 145.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686748 reads in 125.725 CPU sec, 4.002 real sec
[M::mem_process_seqs] Processed 688068 reads in 79.990 CPU sec, 2.521 real sec
[M::process] read 687318 sequences (100000097 bp) in 1.542 CPU sec, 1.592 real sec...
sorting block 27, #reads = 1073323 mem = 536881403 peak = 958705664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285474, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (307.19, 147.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686852 sequences (100000248 bp) in 2.157 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285258, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 144, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412)
[M::mem_pestat] mean and std.dev: (194.20, 95.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 578)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (305.96, 145.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 687718 reads in 128.367 CPU sec, 4.088 real sec
sorting block 28, #reads = 1073289 mem = 536879439 peak = 958705664
[M::process] read 687174 sequences (100000254 bp) in 1.427 CPU sec, 1.443 real sec...
[M::mem_process_seqs] Processed 687318 reads in 81.286 CPU sec, 2.571 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284982, 0, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 118, 163)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 253)
[M::mem_pestat] mean and std.dev: (129.00, 21.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (306.21, 144.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 29, #reads = 1073807 mem = 536877415 peak = 1008967680
[M::process] read 686870 sequences (100000247 bp) in 2.278 CPU sec, 2.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284948, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (303.74, 143.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686852 reads in 130.095 CPU sec, 4.148 real sec
[M::process] read 689922 sequences (100000290 bp) in 1.385 CPU sec, 1.385 real sec...
[M::mem_process_seqs] Processed 687174 reads in 83.923 CPU sec, 2.666 real sec
sorting block 30, #reads = 1072958 mem = 536880591 peak = 1008967680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285096, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (306.10, 145.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687856 sequences (100000047 bp) in 2.082 CPU sec, 2.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285968, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 282, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (304.21, 149.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687040 sequences (100000250 bp) in 1.290 CPU sec, 1.290 real sec...
[M::mem_process_seqs] Processed 686870 reads in 130.506 CPU sec, 4.155 real sec
[M::mem_process_seqs] Processed 689922 reads in 81.658 CPU sec, 2.584 real sec
sorting block 31, #reads = 1071806 mem = 536879424 peak = 1008967680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285968, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 291, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (309.57, 148.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 263, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482)
[M::mem_pestat] mean and std.dev: (231.00, 63.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] skip orientation RR
[M::process] read 686432 sequences (100000165 bp) in 2.315 CPU sec, 2.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285236, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 292, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (308.81, 145.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687856 reads in 123.503 CPU sec, 3.931 real sec
[M::mem_process_seqs] Processed 687040 reads in 78.476 CPU sec, 2.480 real sec
[M::process] read 687624 sequences (100000271 bp) in 1.587 CPU sec, 1.617 real sec...
sorting block 32, #reads = 1074263 mem = 536880249 peak = 1008967680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284686, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 294, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (311.20, 146.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 323, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (280.70, 112.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RR
sorting block 33, #reads = 1074260 mem = 536878653 peak = 1008967680
[M::process] read 687580 sequences (100000073 bp) in 2.223 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285486, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 286, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (304.19, 143.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686432 reads in 128.569 CPU sec, 4.098 real sec
[M::process] read 687208 sequences (100000163 bp) in 1.489 CPU sec, 1.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285318, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (305.52, 145.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 245, 1262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3544)
[M::mem_pestat] mean and std.dev: (449.23, 457.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4685)
[M::mem_pestat] skip orientation RR
[M::process] read 687108 sequences (100000025 bp) in 1.534 CPU sec, 1.538 real sec...
[M::mem_process_seqs] Processed 687580 reads in 77.331 CPU sec, 2.554 real sec
sorting block 34, #reads = 1072240 mem = 536880755 peak = 1008967680
[M::mem_process_seqs] Processed 687624 reads in 183.261 CPU sec, 5.918 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285478, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 149, 174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (72, 242)
[M::mem_pestat] mean and std.dev: (157.07, 33.52)
[M::mem_pestat] low and high boundaries for proper pairs: (23, 291)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (306.07, 144.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 35, #reads = 1072712 mem = 536878444 peak = 1008967680
[M::process] read 686616 sequences (100000223 bp) in 2.217 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285677, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (307.73, 145.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687208 reads in 138.014 CPU sec, 4.394 real sec
[M::mem_process_seqs] Processed 687108 reads in 79.954 CPU sec, 2.531 real sec
[M::process] read 686514 sequences (100000261 bp) in 1.957 CPU sec, 2.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284670, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 149, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (164.82, 65.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (310.38, 148.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 36, #reads = 1074250 mem = 536878884 peak = 1008967680
[M::process] read 688974 sequences (100000270 bp) in 2.081 CPU sec, 2.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284241, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 290, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (309.41, 147.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 342, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1455)
[M::mem_pestat] mean and std.dev: (330.75, 181.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1880)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686616 reads in 130.389 CPU sec, 4.156 real sec
[M::mem_process_seqs] Processed 686514 reads in 84.730 CPU sec, 2.677 real sec
[M::process] read 687642 sequences (100000254 bp) in 1.592 CPU sec, 1.663 real sec...
sorting block 37, #reads = 1073245 mem = 536878187 peak = 1008967680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286024, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (82, 118, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (138.54, 70.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (304.63, 148.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687026 sequences (100000267 bp) in 2.248 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285422, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (308.69, 147.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688974 reads in 133.291 CPU sec, 4.246 real sec
[M::process] read 687088 sequences (100000189 bp) in 1.367 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 687642 reads in 82.901 CPU sec, 2.620 real sec
sorting block 38, #reads = 1069748 mem = 536878803 peak = 1008967680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285044, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 131, 264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (177.73, 81.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 699)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 293, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (310.22, 146.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 247, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427)
[M::mem_pestat] mean and std.dev: (209.89, 49.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 516)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688206 sequences (100000216 bp) in 2.404 CPU sec, 2.534 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285796, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (309.99, 147.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687026 reads in 127.577 CPU sec, 4.059 real sec
[M::mem_process_seqs] Processed 687088 reads in 82.699 CPU sec, 2.612 real sec
sorting block 39, #reads = 1074014 mem = 536877947 peak = 1008967680
[M::process] read 688020 sequences (100000167 bp) in 1.564 CPU sec, 1.593 real sec...
sorting block 40, #reads = 1073102 mem = 536877192 peak = 1054842880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286396, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (302.62, 144.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687030 sequences (100000259 bp) in 2.269 CPU sec, 2.411 real sec...
[M::process] read 687196 sequences (100000217 bp) in 1.310 CPU sec, 1.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285679, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (303.67, 144.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (90, 133, 166)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318)
[M::mem_pestat] mean and std.dev: (130.60, 45.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 394)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688206 reads in 164.168 CPU sec, 5.236 real sec
[M::mem_process_seqs] Processed 688020 reads in 103.584 CPU sec, 3.297 real sec
sorting block 41, #reads = 1064206 mem = 536880087 peak = 1054842880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285560, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 288, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (306.43, 145.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686696 sequences (100000047 bp) in 2.075 CPU sec, 2.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285384, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (306.15, 145.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687498 sequences (100000277 bp) in 1.380 CPU sec, 1.387 real sec...
[M::mem_process_seqs] Processed 687030 reads in 136.567 CPU sec, 4.342 real sec
[M::mem_process_seqs] Processed 687196 reads in 86.906 CPU sec, 2.755 real sec
sorting block 42, #reads = 1067998 mem = 536877826 peak = 1054842880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284687, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (105, 153, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387)
[M::mem_pestat] mean and std.dev: (154.33, 56.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 481)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (310.72, 148.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (115, 153, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400)
[M::mem_pestat] mean and std.dev: (157.10, 57.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 495)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689854 sequences (100000135 bp) in 2.416 CPU sec, 2.549 real sec...
[M::process] read 687906 sequences (100000265 bp) in 1.320 CPU sec, 1.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284955, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (307.45, 148.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 191, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480)
[M::mem_pestat] mean and std.dev: (180.10, 75.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686696 reads in 149.336 CPU sec, 4.750 real sec
sorting block 43, #reads = 1067825 mem = 536877870 peak = 1054842880
[M::mem_process_seqs] Processed 687498 reads in 98.623 CPU sec, 3.133 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286235, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (301.53, 147.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (105, 239, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (224.25, 125.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] skip orientation RR
sorting block 44, #reads = 1063585 mem = 536878364 peak = 1054842880
[M::process] read 686734 sequences (100000234 bp) in 2.397 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285309, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (308.33, 148.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689854 reads in 129.855 CPU sec, 4.126 real sec
[M::process] read 687698 sequences (100000200 bp) in 1.348 CPU sec, 1.352 real sec...
[M::mem_process_seqs] Processed 687906 reads in 81.500 CPU sec, 2.575 real sec
sorting block 45, #reads = 1074160 mem = 536878871 peak = 1054842880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284992, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (308.61, 145.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686800 sequences (100000277 bp) in 2.373 CPU sec, 2.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285922, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 286, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (303.91, 142.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686734 reads in 131.787 CPU sec, 4.188 real sec
[M::process] read 686768 sequences (100000095 bp) in 1.340 CPU sec, 1.340 real sec...
[M::mem_process_seqs] Processed 687698 reads in 87.540 CPU sec, 2.770 real sec
sorting block 46, #reads = 1072412 mem = 536878756 peak = 1054842880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285182, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (309.23, 146.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (105, 146, 175)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315)
[M::mem_pestat] mean and std.dev: (144.70, 41.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 385)
[M::mem_pestat] skip orientation RR
[M::process] read 686524 sequences (100000237 bp) in 2.403 CPU sec, 2.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285180, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (310.61, 148.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686800 reads in 129.921 CPU sec, 4.129 real sec
[M::process] read 685892 sequences (100000023 bp) in 1.330 CPU sec, 1.338 real sec...
[M::mem_process_seqs] Processed 686768 reads in 81.701 CPU sec, 2.582 real sec
sorting block 47, #reads = 1071250 mem = 536879898 peak = 1054842880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284883, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 205, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 459)
[M::mem_pestat] mean and std.dev: (217.80, 72.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 550)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 292, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (310.88, 147.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 685570 sequences (100000207 bp) in 2.383 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284345, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 292, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (310.04, 145.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 189, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (237.17, 121.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686524 reads in 128.925 CPU sec, 4.100 real sec
[M::process] read 685676 sequences (100000056 bp) in 1.445 CPU sec, 1.462 real sec...
sorting block 48, #reads = 1073465 mem = 536878380 peak = 1054842880
[M::mem_process_seqs] Processed 685892 reads in 81.220 CPU sec, 2.563 real sec
[M::process] read 4676 sequences (680528 bp) in 0.401 CPU sec, 0.402 real sec...
sorting block 49, #reads = 1072636 mem = 536878322 peak = 1088192512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284789, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 293, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (311.81, 147.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 1941, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 296, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (312.19, 148.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284418, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 313, 558)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394)
[M::mem_pestat] mean and std.dev: (315.44, 166.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1812)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (314.41, 150.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 685570 reads in 126.056 CPU sec, 4.093 real sec
[M::mem_process_seqs] Processed 4676 reads in 68.528 CPU sec, 2.213 real sec
[M::mem_process_seqs] Processed 685676 reads in 80.383 CPU sec, 2.618 real sec
sorting block 50, #reads = 1072930 mem = 536880161 peak = 1088192512
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 54965450
overall: 69869391872 mem 7195.525 user 429.023 sys 447.175 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:B23KGCJLT4.NA12878_c_0002_gDNA_0002_B23KGCJLT4_1.L002\tPU:L002\tSM:NA12878_c_0002_gDNA_0002_B23KGCJLT4_1\tLB:NA12878_c_0002_gDNA_0002_B23KGCJLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R1.fastq.gz NA12878_c_0002_gDNA_0002_B23KGCJLT4_1_R2.fastq.gz
[main] Real time: 447.212 sec; CPU: 7624.551 sec
sorting block 51, #reads = 525783 mem = 263618124 peak = 1088192512
uniq header count 1
merging 52 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 2744086900
output reads: 55239203
bam_mem_sort: 52 calls 20.034 user 0.284 sys 20.402 real
bam_write: 52 calls 16.026 user 0.581 sys 16.816 real
execute: 1 calls 10.256 user 0.655 sys 461.589 real
merge_files: 1 calls 10.237 user 0.637 sys 13.717 real
parse_chunk: 824 calls 26.876 user 7.084 sys 34.632 real
read_chunk: 2227 calls 20.719 user 20.468 sys 189.055 real
sort_block: 1 calls 17.180 user 20.007 sys 183.519 real
write_chunk: 1401 calls 9.903 user 0.436 sys 13.052 real
overall: 2499649536 mem 541.258 user 36.828 sys 461.613 real
ls: cannot access '*.cram': No such file or directory