.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_d0M-T1-TDNA-1_A23MHWWLT4_1;L003;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/15/2d14bb8927c0f2bc69044f262fd113/.command.err
Size: 198.8 KB
Task: DAQ:SENTIEON_BWAMEM (659_d0M-T1-TDNA-1_A23MHWWLT4_1;L003;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_d0M-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1817.123 CPU sec, 63.041 real sec
[M::process] read 677852 sequences (100000014 bp) in 0.977 CPU sec, 0.978 real sec...
[M::process] read 678106 sequences (100000120 bp) in 1.518 CPU sec, 1.528 real sec...
[M::process] read 677776 sequences (100000191 bp) in 1.547 CPU sec, 1.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285851, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 311, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (333.98, 155.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677590 sequences (100000177 bp) in 1.517 CPU sec, 1.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284767, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 311, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (333.62, 154.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285272, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 312, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (333.51, 153.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677852 reads in 253.713 CPU sec, 8.186 real sec
[M::mem_process_seqs] Processed 678106 reads in 166.050 CPU sec, 5.416 real sec
[M::mem_process_seqs] Processed 677776 reads in 180.250 CPU sec, 5.886 real sec
sorting block 0, #reads = 1083084 mem = 536877432 peak = 589127680
[M::process] read 677540 sequences (100000091 bp) in 2.239 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285268, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 315, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (335.67, 154.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677390 sequences (100000113 bp) in 1.645 CPU sec, 1.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284972, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (337.46, 155.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677590 reads in 152.459 CPU sec, 4.834 real sec
sorting block 1, #reads = 1083032 mem = 536878253 peak = 829775872
[M::mem_process_seqs] Processed 677540 reads in 95.490 CPU sec, 2.997 real sec
[M::process] read 677212 sequences (100000125 bp) in 1.697 CPU sec, 1.747 real sec...
sorting block 2, #reads = 1082459 mem = 536880212 peak = 849117184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285465, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 319, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (340.32, 156.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678128 sequences (100000186 bp) in 1.963 CPU sec, 2.174 real sec...
[M::process] read 676448 sequences (100000187 bp) in 1.599 CPU sec, 1.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285345, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (344.45, 159.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 201, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (321.50, 186.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677390 reads in 153.734 CPU sec, 4.875 real sec
[M::mem_process_seqs] Processed 677212 reads in 99.504 CPU sec, 3.154 real sec
sorting block 3, #reads = 1082606 mem = 536878595 peak = 849117184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285812, 2, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 243, 894)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2294)
[M::mem_pestat] mean and std.dev: (430.67, 330.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2994)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (336.05, 157.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676098 sequences (100000172 bp) in 2.260 CPU sec, 2.354 real sec...
[M::process] read 675810 sequences (100000146 bp) in 1.643 CPU sec, 1.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285092, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (349.49, 160.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678128 reads in 157.091 CPU sec, 4.975 real sec
[M::mem_process_seqs] Processed 676448 reads in 100.252 CPU sec, 3.180 real sec
sorting block 4, #reads = 1082161 mem = 536878999 peak = 849117184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284686, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (350.16, 160.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675628 sequences (100000114 bp) in 2.342 CPU sec, 2.455 real sec...
[M::process] read 675700 sequences (100000234 bp) in 1.679 CPU sec, 1.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284465, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (354.19, 161.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676098 reads in 159.115 CPU sec, 5.044 real sec
[M::mem_process_seqs] Processed 675810 reads in 100.866 CPU sec, 3.199 real sec
sorting block 5, #reads = 1083083 mem = 536878694 peak = 849117184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284119, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (353.93, 161.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 408, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (374.42, 125.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RR
[M::process] read 675632 sequences (100000111 bp) in 1.906 CPU sec, 2.019 real sec...
[M::process] read 676264 sequences (100000193 bp) in 1.275 CPU sec, 1.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284917, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 829, 829)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2029)
[M::mem_pestat] mean and std.dev: (645.79, 304.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2629)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (352.81, 160.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675628 reads in 160.207 CPU sec, 5.108 real sec
sorting block 6, #reads = 1081999 mem = 536881698 peak = 849117184
[M::mem_process_seqs] Processed 675700 reads in 103.115 CPU sec, 3.299 real sec
sorting block 7, #reads = 1081797 mem = 536878842 peak = 860385280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284103, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (353.70, 160.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675258 sequences (100000000 bp) in 2.417 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284366, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (345.94, 158.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674840 sequences (100000177 bp) in 1.700 CPU sec, 1.701 real sec...
[M::mem_process_seqs] Processed 675632 reads in 156.520 CPU sec, 4.965 real sec
[M::mem_process_seqs] Processed 676264 reads in 100.885 CPU sec, 3.191 real sec
sorting block 8, #reads = 1079989 mem = 536877176 peak = 860385280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284415, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (351.75, 159.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674972 sequences (100000077 bp) in 2.431 CPU sec, 2.550 real sec...
[M::process] read 674744 sequences (100000043 bp) in 1.720 CPU sec, 1.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284162, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 336, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (354.79, 158.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675258 reads in 158.088 CPU sec, 5.009 real sec
[M::mem_process_seqs] Processed 674840 reads in 100.014 CPU sec, 3.163 real sec
sorting block 9, #reads = 1081436 mem = 536880268 peak = 860385280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284354, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (356.96, 161.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674562 sequences (100000237 bp) in 2.549 CPU sec, 2.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284038, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (357.05, 160.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674504 sequences (100000070 bp) in 1.840 CPU sec, 1.838 real sec...
[M::mem_process_seqs] Processed 674972 reads in 157.082 CPU sec, 4.980 real sec
sorting block 10, #reads = 1081320 mem = 536877403 peak = 860385280
[M::mem_process_seqs] Processed 674744 reads in 100.837 CPU sec, 3.187 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283737, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (355.40, 158.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674626 sequences (100000038 bp) in 2.600 CPU sec, 2.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284556, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 281, 671)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1583)
[M::mem_pestat] mean and std.dev: (394.00, 200.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2039)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 337, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (355.05, 158.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674816 sequences (100000289 bp) in 1.751 CPU sec, 1.753 real sec...
[M::mem_process_seqs] Processed 674562 reads in 159.792 CPU sec, 5.065 real sec
sorting block 11, #reads = 1082185 mem = 536878214 peak = 860385280
[M::mem_process_seqs] Processed 674504 reads in 103.472 CPU sec, 3.273 real sec
sorting block 12, #reads = 1080694 mem = 536878568 peak = 938528768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283822, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (354.23, 158.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675044 sequences (100000045 bp) in 2.508 CPU sec, 2.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284036, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (352.18, 158.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674856 sequences (100000167 bp) in 1.770 CPU sec, 1.778 real sec...
[M::mem_process_seqs] Processed 674626 reads in 160.091 CPU sec, 5.080 real sec
[M::mem_process_seqs] Processed 674816 reads in 102.930 CPU sec, 3.255 real sec
sorting block 13, #reads = 1080474 mem = 536881520 peak = 938528768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284164, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (489, 533, 1642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3948)
[M::mem_pestat] mean and std.dev: (814.10, 552.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5101)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (351.44, 157.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674844 sequences (100000047 bp) in 2.559 CPU sec, 2.670 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283969, 16, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 330, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (349.53, 157.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (489, 5420, 5420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15282)
[M::mem_pestat] mean and std.dev: (3001.62, 2448.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20213)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 674524 sequences (100000003 bp) in 1.708 CPU sec, 1.717 real sec...
[M::mem_process_seqs] Processed 675044 reads in 158.059 CPU sec, 5.011 real sec
[M::mem_process_seqs] Processed 674856 reads in 100.458 CPU sec, 3.175 real sec
sorting block 14, #reads = 1081455 mem = 536879428 peak = 938528768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284450, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (352.08, 157.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674666 sequences (100000033 bp) in 2.620 CPU sec, 2.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284202, 11, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 267, 1152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3202)
[M::mem_pestat] mean and std.dev: (529.29, 538.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4227)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 337, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (355.69, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 1068, 2105)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6223)
[M::mem_pestat] mean and std.dev: (1153.45, 1016.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8282)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 409, 2825)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8179)
[M::mem_pestat] mean and std.dev: (1049.60, 1168.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10856)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 674352 sequences (100000233 bp) in 1.720 CPU sec, 1.728 real sec...
[M::mem_process_seqs] Processed 674844 reads in 156.645 CPU sec, 4.967 real sec
sorting block 15, #reads = 1080416 mem = 536879205 peak = 938528768
[M::mem_process_seqs] Processed 674524 reads in 100.107 CPU sec, 3.157 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284389, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (354.45, 159.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674524 sequences (100000251 bp) in 2.654 CPU sec, 2.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284401, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (356.44, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677032 sequences (100000222 bp) in 1.800 CPU sec, 1.795 real sec...
[M::mem_process_seqs] Processed 674666 reads in 155.418 CPU sec, 4.921 real sec
sorting block 16, #reads = 1081133 mem = 536879110 peak = 938528768
[M::mem_process_seqs] Processed 674352 reads in 99.098 CPU sec, 3.125 real sec
sorting block 17, #reads = 1081650 mem = 536880114 peak = 972267520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284183, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 340, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (358.49, 161.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (667, 1892, 1892)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4342)
[M::mem_pestat] mean and std.dev: (1377.25, 742.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5567)
[M::mem_pestat] skip orientation RR
[M::process] read 677836 sequences (100000209 bp) in 2.417 CPU sec, 2.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284571, 0, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 324, 3332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9622)
[M::mem_pestat] mean and std.dev: (1140.75, 1310.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12767)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 319, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (341.52, 157.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677612 sequences (100000149 bp) in 1.756 CPU sec, 1.756 real sec...
[M::mem_process_seqs] Processed 674524 reads in 157.470 CPU sec, 5.000 real sec
[M::mem_process_seqs] Processed 677032 reads in 103.307 CPU sec, 3.257 real sec
sorting block 18, #reads = 1080909 mem = 536878075 peak = 972267520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285128, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 314, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (336.35, 156.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (379, 379, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (379, 379)
[M::mem_pestat] mean and std.dev: (379.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (379, 379)
[M::mem_pestat] skip orientation RR
[M::process] read 677564 sequences (100000042 bp) in 2.476 CPU sec, 2.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283773, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (338.20, 156.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677442 sequences (100000246 bp) in 1.729 CPU sec, 1.737 real sec...
[M::mem_process_seqs] Processed 677836 reads in 157.870 CPU sec, 5.011 real sec
[M::mem_process_seqs] Processed 677612 reads in 100.683 CPU sec, 3.181 real sec
sorting block 19, #reads = 1082002 mem = 536879344 peak = 972267520
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284735, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 1091, 4087)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11963)
[M::mem_pestat] mean and std.dev: (1600.64, 1612.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15901)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (340.78, 157.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677160 sequences (100000295 bp) in 2.644 CPU sec, 2.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285327, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 320, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (340.47, 156.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677008 sequences (100000157 bp) in 1.765 CPU sec, 1.769 real sec...
[M::mem_process_seqs] Processed 677564 reads in 159.267 CPU sec, 5.042 real sec
sorting block 20, #reads = 1082754 mem = 536880781 peak = 972267520
[M::mem_process_seqs] Processed 677442 reads in 101.362 CPU sec, 3.200 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284481, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (342.75, 157.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676716 sequences (100000280 bp) in 2.647 CPU sec, 2.762 real sec...
[M::process] read 676390 sequences (100000059 bp) in 1.620 CPU sec, 1.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284299, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (343.38, 157.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677160 reads in 160.955 CPU sec, 5.101 real sec
sorting block 21, #reads = 1082712 mem = 536879974 peak = 972267520
[M::mem_process_seqs] Processed 677008 reads in 102.921 CPU sec, 3.261 real sec
sorting block 22, #reads = 1082662 mem = 536875802 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285441, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (347.38, 159.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675852 sequences (100000054 bp) in 2.712 CPU sec, 2.818 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285382, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (352.30, 162.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (293, 332, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 689)
[M::mem_pestat] mean and std.dev: (338.70, 110.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RR
[M::process] read 675990 sequences (100000000 bp) in 1.757 CPU sec, 1.760 real sec...
[M::mem_process_seqs] Processed 676716 reads in 156.208 CPU sec, 4.955 real sec
[M::mem_process_seqs] Processed 676390 reads in 101.653 CPU sec, 3.215 real sec
sorting block 23, #reads = 1082447 mem = 536882067 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284628, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (353.28, 161.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675522 sequences (100000071 bp) in 2.487 CPU sec, 2.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284510, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (355.41, 162.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675726 sequences (100000164 bp) in 1.780 CPU sec, 1.774 real sec...
[M::mem_process_seqs] Processed 675852 reads in 154.819 CPU sec, 4.910 real sec
[M::mem_process_seqs] Processed 675990 reads in 100.147 CPU sec, 3.162 real sec
sorting block 24, #reads = 1083727 mem = 536876245 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284069, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (358.10, 163.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675368 sequences (100000240 bp) in 2.715 CPU sec, 2.835 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284052, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (356.40, 162.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675132 sequences (100000118 bp) in 1.753 CPU sec, 1.762 real sec...
[M::mem_process_seqs] Processed 675522 reads in 160.268 CPU sec, 5.078 real sec
sorting block 25, #reads = 1081671 mem = 536880347 peak = 1004171264
[M::mem_process_seqs] Processed 675726 reads in 103.219 CPU sec, 3.262 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284098, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (356.42, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1080905 mem = 536877144 peak = 1004171264
[M::process] read 675580 sequences (100000288 bp) in 2.686 CPU sec, 2.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284428, 9, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 158, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (179.67, 62.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (358.08, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675002 sequences (100000011 bp) in 1.746 CPU sec, 1.752 real sec...
[M::mem_process_seqs] Processed 675368 reads in 159.379 CPU sec, 5.054 real sec
[M::mem_process_seqs] Processed 675132 reads in 100.477 CPU sec, 3.171 real sec
sorting block 27, #reads = 1080832 mem = 536880354 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284318, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (353.70, 159.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674864 sequences (100000008 bp) in 2.839 CPU sec, 2.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284069, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (356.80, 160.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675580 reads in 156.341 CPU sec, 4.962 real sec
[M::mem_process_seqs] Processed 675002 reads in 99.855 CPU sec, 3.144 real sec
[M::process] read 674778 sequences (100000260 bp) in 2.202 CPU sec, 2.256 real sec...
sorting block 28, #reads = 1081841 mem = 536880338 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283999, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (357.42, 160.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674644 sequences (100000236 bp) in 2.999 CPU sec, 3.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284399, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 411, 1221)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3271)
[M::mem_pestat] mean and std.dev: (537.67, 438.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4296)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 338, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (355.28, 158.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674864 reads in 158.086 CPU sec, 5.038 real sec
[M::mem_process_seqs] Processed 674778 reads in 101.825 CPU sec, 3.209 real sec
[M::process] read 674630 sequences (100000065 bp) in 2.271 CPU sec, 2.359 real sec...
sorting block 29, #reads = 1081752 mem = 536878456 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284271, 3, 20)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 340, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (358.38, 161.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (466, 466, 563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (272, 757)
[M::mem_pestat] mean and std.dev: (460.79, 103.67)
[M::mem_pestat] low and high boundaries for proper pairs: (46, 875)
[M::mem_pestat] skip orientation RR
[M::process] read 674678 sequences (100000184 bp) in 2.453 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284200, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (356.02, 158.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674644 reads in 159.082 CPU sec, 5.049 real sec
[M::process] read 674952 sequences (100000022 bp) in 1.779 CPU sec, 1.799 real sec...
[M::mem_process_seqs] Processed 674630 reads in 102.131 CPU sec, 3.210 real sec
sorting block 30, #reads = 1081155 mem = 536878196 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284019, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 336, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (353.97, 157.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1081561 mem = 536878699 peak = 1004171264
[M::process] read 675032 sequences (100000128 bp) in 2.499 CPU sec, 2.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284146, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (351.21, 156.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674678 reads in 161.305 CPU sec, 5.129 real sec
[M::process] read 674864 sequences (100000005 bp) in 1.889 CPU sec, 1.900 real sec...
[M::mem_process_seqs] Processed 674952 reads in 103.618 CPU sec, 3.271 real sec
sorting block 32, #reads = 1080700 mem = 536880858 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284267, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (351.99, 157.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675120 sequences (100000276 bp) in 2.739 CPU sec, 2.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284609, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (353.16, 158.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674912 sequences (100000065 bp) in 1.788 CPU sec, 1.807 real sec...
[M::mem_process_seqs] Processed 675032 reads in 163.670 CPU sec, 5.191 real sec
[M::mem_process_seqs] Processed 674864 reads in 105.161 CPU sec, 3.319 real sec
sorting block 33, #reads = 1080732 mem = 536881202 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284517, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (352.74, 158.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674920 sequences (100000009 bp) in 2.774 CPU sec, 2.905 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284145, 7, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 166, 178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (142, 202)
[M::mem_pestat] mean and std.dev: (168.10, 8.24)
[M::mem_pestat] low and high boundaries for proper pairs: (130, 214)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (351.78, 157.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675120 reads in 159.422 CPU sec, 5.066 real sec
[M::process] read 675070 sequences (100000026 bp) in 1.969 CPU sec, 2.008 real sec...
[M::mem_process_seqs] Processed 674912 reads in 101.434 CPU sec, 3.191 real sec
sorting block 34, #reads = 1081136 mem = 536885251 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284778, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 1399, 1399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3647)
[M::mem_pestat] mean and std.dev: (983.64, 618.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4771)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 335, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (353.53, 159.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674662 sequences (100000068 bp) in 2.556 CPU sec, 2.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284953, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (351.33, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674920 reads in 155.628 CPU sec, 4.958 real sec
[M::mem_process_seqs] Processed 675070 reads in 100.162 CPU sec, 3.150 real sec
sorting block 35, #reads = 1081043 mem = 536878772 peak = 1004171264
[M::process] read 675276 sequences (100000299 bp) in 2.080 CPU sec, 2.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284085, 1, 18)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (357.90, 162.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 932, 1429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3761)
[M::mem_pestat] mean and std.dev: (875.00, 684.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4927)
[M::mem_pestat] skip orientation RR
sorting block 36, #reads = 1081002 mem = 536878162 peak = 1004171264
[M::process] read 678510 sequences (100000228 bp) in 2.678 CPU sec, 2.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284772, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (354.29, 161.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674662 reads in 157.354 CPU sec, 5.013 real sec
[M::mem_process_seqs] Processed 675276 reads in 102.598 CPU sec, 3.228 real sec
[M::process] read 678032 sequences (100000054 bp) in 1.938 CPU sec, 1.975 real sec...
sorting block 37, #reads = 1080665 mem = 536879000 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285769, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 311, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (334.38, 156.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677800 sequences (100000248 bp) in 2.599 CPU sec, 2.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285358, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 314, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (336.21, 156.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678510 reads in 161.581 CPU sec, 5.142 real sec
[M::process] read 677512 sequences (100000102 bp) in 1.941 CPU sec, 1.950 real sec...
[M::mem_process_seqs] Processed 678032 reads in 105.016 CPU sec, 3.314 real sec
sorting block 38, #reads = 1081702 mem = 536883400 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285684, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (338.41, 156.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677322 sequences (100000070 bp) in 2.593 CPU sec, 2.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285281, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 319, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (339.28, 156.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677800 reads in 164.104 CPU sec, 5.234 real sec
[M::process] read 677150 sequences (100000141 bp) in 1.994 CPU sec, 2.030 real sec...
[M::mem_process_seqs] Processed 677512 reads in 106.138 CPU sec, 3.349 real sec
sorting block 39, #reads = 1081990 mem = 536880966 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285449, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 320, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (339.99, 155.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676738 sequences (100000272 bp) in 2.644 CPU sec, 2.798 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285518, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (342.38, 156.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677322 reads in 165.966 CPU sec, 5.314 real sec
[M::process] read 676458 sequences (100000126 bp) in 1.917 CPU sec, 1.935 real sec...
[M::mem_process_seqs] Processed 677150 reads in 108.431 CPU sec, 3.479 real sec
sorting block 40, #reads = 1081911 mem = 536881019 peak = 1004171264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285228, 10, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (345.84, 158.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (537, 4108, 5954)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16788)
[M::mem_pestat] mean and std.dev: (2833.90, 2468.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22205)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 285, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (251.73, 84.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 707)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 41, #reads = 1082688 mem = 536879737 peak = 1004171264
[M::process] read 676370 sequences (100000049 bp) in 2.817 CPU sec, 3.075 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285080, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.69, 158.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676738 reads in 167.457 CPU sec, 5.394 real sec
[M::mem_process_seqs] Processed 676458 reads in 108.093 CPU sec, 3.465 real sec
[M::process] read 675868 sequences (100000044 bp) in 2.065 CPU sec, 2.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285108, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 289, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (224.91, 73.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (352.52, 162.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 42, #reads = 1081937 mem = 536878500 peak = 1004171264
[M::process] read 675564 sequences (100000288 bp) in 2.712 CPU sec, 3.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285020, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (354.60, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676370 reads in 168.339 CPU sec, 5.428 real sec
[M::process] read 675518 sequences (100000183 bp) in 2.355 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284840, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (357.63, 164.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675564 reads in 92.947 CPU sec, 3.003 real sec
sorting block 43, #reads = 1082138 mem = 536878413 peak = 1004171264
[M::process] read 675598 sequences (100000126 bp) in 2.169 CPU sec, 2.352 real sec...
[M::mem_process_seqs] Processed 675868 reads in 265.438 CPU sec, 8.598 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284591, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (356.44, 163.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1082203 mem = 536880136 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284767, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (357.10, 162.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675524 sequences (100000227 bp) in 2.568 CPU sec, 2.992 real sec...
[M::mem_process_seqs] Processed 675518 reads in 187.680 CPU sec, 6.211 real sec
[M::mem_process_seqs] Processed 675598 reads in 111.436 CPU sec, 3.734 real sec
[M::process] read 675414 sequences (100000139 bp) in 2.654 CPU sec, 2.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285010, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 338, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (357.70, 164.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1081204 mem = 536879044 peak = 1039814656
[M::process] read 675528 sequences (100000117 bp) in 2.509 CPU sec, 2.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284596, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (357.15, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675524 reads in 172.124 CPU sec, 5.731 real sec
[M::mem_process_seqs] Processed 675414 reads in 111.367 CPU sec, 3.673 real sec
[M::process] read 675556 sequences (100000221 bp) in 2.317 CPU sec, 2.658 real sec...
sorting block 46, #reads = 1081515 mem = 536877553 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285333, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (355.60, 162.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284612, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (353.71, 161.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675494 sequences (100000218 bp) in 2.546 CPU sec, 2.980 real sec...
[M::mem_process_seqs] Processed 675528 reads in 172.206 CPU sec, 5.825 real sec
[M::mem_process_seqs] Processed 675556 reads in 110.629 CPU sec, 3.668 real sec
sorting block 47, #reads = 1080817 mem = 536882858 peak = 1039814656
[M::process] read 675532 sequences (100000006 bp) in 2.573 CPU sec, 2.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284310, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (354.45, 162.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675494 reads in 96.788 CPU sec, 3.602 real sec
[M::process] read 675676 sequences (100000255 bp) in 2.249 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284709, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (352.90, 161.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1082334 mem = 536878462 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284199, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 675438 sequences (100000021 bp) in 2.376 CPU sec, 2.845 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (350.12, 159.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675532 reads in 175.946 CPU sec, 5.819 real sec
[M::mem_process_seqs] Processed 675676 reads in 113.775 CPU sec, 3.726 real sec
sorting block 49, #reads = 1080913 mem = 536878502 peak = 1039814656
[M::process] read 675588 sequences (100000009 bp) in 2.653 CPU sec, 2.930 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284675, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (349.26, 159.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284358, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.13, 160.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675510 sequences (100000095 bp) in 2.442 CPU sec, 3.013 real sec...
[M::mem_process_seqs] Processed 675438 reads in 175.711 CPU sec, 5.895 real sec
sorting block 50, #reads = 1081144 mem = 536878332 peak = 1039814656
[M::process] read 675166 sequences (100000005 bp) in 2.341 CPU sec, 2.932 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285007, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (350.75, 159.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675510 reads in 96.375 CPU sec, 3.359 real sec
[M::process] read 675248 sequences (100000053 bp) in 2.452 CPU sec, 2.593 real sec...
sorting block 51, #reads = 1080767 mem = 536878339 peak = 1039814656
[M::mem_process_seqs] Processed 675588 reads in 276.342 CPU sec, 9.318 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283755, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (350.13, 157.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284325, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (298, 669, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1411)
[M::mem_pestat] mean and std.dev: (526.33, 240.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1782)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (352.18, 159.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675066 sequences (100000025 bp) in 2.487 CPU sec, 2.977 real sec...
[M::mem_process_seqs] Processed 675166 reads in 185.191 CPU sec, 5.974 real sec
[M::mem_process_seqs] Processed 675248 reads in 109.872 CPU sec, 3.577 real sec
sorting block 52, #reads = 1080413 mem = 536883011 peak = 1039814656
[M::process] read 675076 sequences (100000119 bp) in 2.661 CPU sec, 2.892 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284205, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 640, 723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853)
[M::mem_pestat] mean and std.dev: (363.43, 252.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2418)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (353.34, 160.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284559, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (354.12, 160.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675118 sequences (100000136 bp) in 2.555 CPU sec, 2.769 real sec...
[M::mem_process_seqs] Processed 675066 reads in 162.369 CPU sec, 5.242 real sec
sorting block 53, #reads = 1081155 mem = 536877986 peak = 1039814656
[M::mem_process_seqs] Processed 675076 reads in 106.518 CPU sec, 3.398 real sec
[M::process] read 674922 sequences (100000075 bp) in 2.458 CPU sec, 2.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284294, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (353.70, 160.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1081286 mem = 536880045 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284186, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (352.83, 160.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (413, 413, 849)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1721)
[M::mem_pestat] mean and std.dev: (581.80, 218.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2157)
[M::mem_pestat] skip orientation RR
[M::process] read 675282 sequences (100000291 bp) in 2.684 CPU sec, 2.988 real sec...
[M::mem_process_seqs] Processed 675118 reads in 163.691 CPU sec, 5.276 real sec
[M::mem_process_seqs] Processed 674922 reads in 105.967 CPU sec, 3.418 real sec
[M::process] read 678128 sequences (100000225 bp) in 2.486 CPU sec, 2.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284887, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (352.71, 160.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 55, #reads = 1081231 mem = 536885196 peak = 1039814656
[M::process] read 677678 sequences (100000178 bp) in 2.555 CPU sec, 2.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285331, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 424, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449)
[M::mem_pestat] mean and std.dev: (403.20, 219.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1869)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 315, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (338.60, 159.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675282 reads in 166.161 CPU sec, 5.339 real sec
[M::mem_process_seqs] Processed 678128 reads in 108.454 CPU sec, 3.450 real sec
[M::process] read 677462 sequences (100000138 bp) in 2.143 CPU sec, 2.229 real sec...
sorting block 56, #reads = 1080012 mem = 536877878 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284624, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 318, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (341.72, 160.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676888 sequences (100000043 bp) in 2.515 CPU sec, 2.746 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285141, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 320, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (343.00, 160.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677678 reads in 156.772 CPU sec, 5.045 real sec
[M::mem_process_seqs] Processed 677462 reads in 103.160 CPU sec, 3.272 real sec
sorting block 57, #reads = 1082785 mem = 536878806 peak = 1039814656
[M::process] read 677084 sequences (100000202 bp) in 2.334 CPU sec, 2.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285463, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (346.13, 161.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1082970 mem = 536877885 peak = 1039814656
[M::process] read 676942 sequences (100000295 bp) in 2.587 CPU sec, 2.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284955, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (346.50, 161.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676888 reads in 159.529 CPU sec, 5.096 real sec
[M::mem_process_seqs] Processed 677084 reads in 106.649 CPU sec, 3.363 real sec
[M::process] read 676520 sequences (100000119 bp) in 2.126 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284890, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (347.32, 161.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 59, #reads = 1082627 mem = 536878705 peak = 1039814656
[M::process] read 676064 sequences (100000062 bp) in 2.697 CPU sec, 2.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284791, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (349.33, 161.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676942 reads in 162.977 CPU sec, 5.212 real sec
[M::mem_process_seqs] Processed 676520 reads in 107.409 CPU sec, 3.395 real sec
[M::process] read 676110 sequences (100000250 bp) in 2.209 CPU sec, 2.298 real sec...
sorting block 60, #reads = 1082621 mem = 536878064 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284601, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (351.50, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675988 sequences (100000045 bp) in 2.545 CPU sec, 2.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284787, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (352.72, 162.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676064 reads in 161.003 CPU sec, 5.141 real sec
[M::mem_process_seqs] Processed 676110 reads in 105.293 CPU sec, 3.320 real sec
[M::process] read 675714 sequences (100000292 bp) in 1.845 CPU sec, 1.882 real sec...
sorting block 61, #reads = 1082949 mem = 536876965 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284442, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (354.18, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675776 sequences (100000029 bp) in 2.814 CPU sec, 3.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284897, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (357.03, 164.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675988 reads in 163.435 CPU sec, 5.246 real sec
[M::mem_process_seqs] Processed 675714 reads in 109.060 CPU sec, 3.481 real sec
[M::process] read 675534 sequences (100000091 bp) in 2.104 CPU sec, 2.158 real sec...
sorting block 62, #reads = 1082472 mem = 536879456 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285121, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (357.34, 165.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1082801 mem = 536880929 peak = 1039814656
[M::process] read 675592 sequences (100000261 bp) in 2.564 CPU sec, 3.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284919, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 338, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (359.17, 166.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675776 reads in 165.905 CPU sec, 5.429 real sec
[M::mem_process_seqs] Processed 675534 reads in 110.041 CPU sec, 3.588 real sec
[M::process] read 675726 sequences (100000005 bp) in 2.393 CPU sec, 2.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284533, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 338, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (359.43, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 64, #reads = 1082392 mem = 536878560 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284464, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (355.39, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675756 sequences (100000267 bp) in 2.622 CPU sec, 3.159 real sec...
[M::mem_process_seqs] Processed 675592 reads in 168.995 CPU sec, 5.556 real sec
[M::mem_process_seqs] Processed 675726 reads in 110.226 CPU sec, 3.598 real sec
[M::process] read 675944 sequences (100000012 bp) in 2.638 CPU sec, 3.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284507, 4, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 168, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (76, 306)
[M::mem_pestat] mean and std.dev: (172.09, 26.17)
[M::mem_pestat] low and high boundaries for proper pairs: (30, 352)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (355.31, 165.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 65, #reads = 1081433 mem = 536879355 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284707, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (353.61, 164.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675980 sequences (100000123 bp) in 2.431 CPU sec, 2.701 real sec...
[M::mem_process_seqs] Processed 675756 reads in 173.028 CPU sec, 5.679 real sec
[M::mem_process_seqs] Processed 675944 reads in 111.460 CPU sec, 3.703 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285173, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (352.60, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 66, #reads = 1081875 mem = 536880879 peak = 1039814656
[M::process] read 676286 sequences (100000127 bp) in 2.670 CPU sec, 3.013 real sec...
[M::mem_process_seqs] Processed 675980 reads in 94.723 CPU sec, 3.296 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284710, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (350.11, 162.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676218 sequences (100000091 bp) in 2.660 CPU sec, 2.935 real sec...
[M::mem_process_seqs] Processed 676286 reads in 95.096 CPU sec, 3.276 real sec
sorting block 67, #reads = 1082255 mem = 536878090 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284667, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 1041, 1041)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2811)
[M::mem_pestat] mean and std.dev: (658.40, 427.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3696)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (349.84, 162.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676112 sequences (100000284 bp) in 2.473 CPU sec, 2.811 real sec...
[M::mem_process_seqs] Processed 676218 reads in 94.691 CPU sec, 3.272 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285088, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (348.37, 161.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676102 sequences (100000161 bp) in 2.513 CPU sec, 2.863 real sec...
sorting block 68, #reads = 1082160 mem = 536880681 peak = 1039814656
[M::mem_process_seqs] Processed 676112 reads in 94.077 CPU sec, 3.253 real sec
[M::process] read 676050 sequences (100000300 bp) in 2.313 CPU sec, 2.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285193, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (349.74, 162.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676102 reads in 93.799 CPU sec, 3.300 real sec
sorting block 69, #reads = 1081127 mem = 536881169 peak = 1039814656
[M::process] read 675810 sequences (100000128 bp) in 2.499 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284342, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (345.74, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676050 reads in 94.323 CPU sec, 3.201 real sec
[M::process] read 675574 sequences (100000006 bp) in 2.487 CPU sec, 2.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284759, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (348.11, 159.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (485, 669, 835)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1535)
[M::mem_pestat] mean and std.dev: (680.92, 273.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1885)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675810 reads in 94.810 CPU sec, 3.207 real sec
sorting block 70, #reads = 1081320 mem = 536877520 peak = 1039814656
[M::process] read 675744 sequences (100000151 bp) in 2.581 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284676, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (350.38, 160.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675574 reads in 93.057 CPU sec, 3.173 real sec
sorting block 71, #reads = 1081560 mem = 536880523 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284327, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (350.48, 160.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675538 sequences (100000089 bp) in 2.681 CPU sec, 3.038 real sec...
[M::mem_process_seqs] Processed 675744 reads in 95.545 CPU sec, 3.269 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284969, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (257, 257, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (219, 314)
[M::mem_pestat] mean and std.dev: (263.33, 8.96)
[M::mem_pestat] low and high boundaries for proper pairs: (200, 333)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (352.33, 161.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675484 sequences (100000003 bp) in 2.553 CPU sec, 2.920 real sec...
[M::mem_process_seqs] Processed 675538 reads in 92.081 CPU sec, 3.165 real sec
sorting block 72, #reads = 1081210 mem = 536881141 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284958, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (353.28, 162.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675464 sequences (100000278 bp) in 2.593 CPU sec, 2.857 real sec...
[M::mem_process_seqs] Processed 675484 reads in 92.901 CPU sec, 3.157 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284783, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.83, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675098 sequences (100000290 bp) in 2.511 CPU sec, 2.797 real sec...
sorting block 73, #reads = 1081055 mem = 536880504 peak = 1039814656
[M::mem_process_seqs] Processed 675464 reads in 91.169 CPU sec, 3.188 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284707, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (354.01, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678064 sequences (100000217 bp) in 2.693 CPU sec, 2.894 real sec...
[M::mem_process_seqs] Processed 675098 reads in 94.737 CPU sec, 3.310 real sec
sorting block 74, #reads = 1081685 mem = 536875661 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285968, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 314, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (340.28, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680078 sequences (100000221 bp) in 2.705 CPU sec, 2.952 real sec...
[M::mem_process_seqs] Processed 678064 reads in 101.065 CPU sec, 3.506 real sec
[M::process] read 679836 sequences (100000156 bp) in 2.524 CPU sec, 2.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286831, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 299, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (328.83, 160.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 75, #reads = 1080983 mem = 536876721 peak = 1039814656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286666, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 301, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (330.04, 159.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679136 sequences (100000079 bp) in 2.681 CPU sec, 2.944 real sec...
[M::mem_process_seqs] Processed 680078 reads in 187.342 CPU sec, 6.143 real sec
[M::mem_process_seqs] Processed 679836 reads in 119.568 CPU sec, 3.881 real sec
sorting block 76, #reads = 1079646 mem = 536878156 peak = 1039814656
[M::process] read 678710 sequences (100000159 bp) in 2.626 CPU sec, 2.966 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286010, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (335.75, 161.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285581, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 310, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (336.89, 160.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678458 sequences (100000081 bp) in 2.545 CPU sec, 3.026 real sec...
[M::mem_process_seqs] Processed 679136 reads in 185.017 CPU sec, 6.095 real sec
[M::mem_process_seqs] Processed 678710 reads in 118.639 CPU sec, 3.921 real sec
sorting block 77, #reads = 1080829 mem = 536877305 peak = 1039814656
[M::process] read 679602 sequences (100000011 bp) in 2.759 CPU sec, 3.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285884, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (339.29, 162.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678458 reads in 101.002 CPU sec, 3.527 real sec
[M::process] read 678212 sequences (100000241 bp) in 2.555 CPU sec, 2.708 real sec...
sorting block 78, #reads = 1081924 mem = 536879857 peak = 1040502784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286255, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 300, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (328.43, 158.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285619, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (340.03, 161.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678192 sequences (100000226 bp) in 2.798 CPU sec, 3.382 real sec...
[M::mem_process_seqs] Processed 679602 reads in 181.631 CPU sec, 5.979 real sec
[M::mem_process_seqs] Processed 678212 reads in 117.740 CPU sec, 3.852 real sec
sorting block 79, #reads = 1082679 mem = 536878994 peak = 1040502784
[M::process] read 677590 sequences (100000069 bp) in 2.647 CPU sec, 2.888 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285612, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (343.36, 163.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285314, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (343.83, 161.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676970 sequences (100000223 bp) in 2.404 CPU sec, 2.718 real sec...
sorting block 80, #reads = 1081713 mem = 536877574 peak = 1040502784
[M::mem_process_seqs] Processed 678192 reads in 181.217 CPU sec, 5.948 real sec
[M::mem_process_seqs] Processed 677590 reads in 115.412 CPU sec, 3.797 real sec
[M::process] read 677138 sequences (100000112 bp) in 2.514 CPU sec, 2.904 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285167, 12, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (106, 147, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (215.30, 152.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 328, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (351.02, 164.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 2366, 2366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6820)
[M::mem_pestat] mean and std.dev: (1619.75, 1218.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9047)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
sorting block 81, #reads = 1080331 mem = 536881400 peak = 1040502784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285419, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 324, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (347.36, 162.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677128 sequences (100000062 bp) in 2.616 CPU sec, 3.129 real sec...
[M::mem_process_seqs] Processed 676970 reads in 186.483 CPU sec, 6.131 real sec
[M::mem_process_seqs] Processed 677138 reads in 117.363 CPU sec, 3.814 real sec
[M::process] read 678408 sequences (100000255 bp) in 2.401 CPU sec, 2.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285420, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 323, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (345.47, 161.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 82, #reads = 1078319 mem = 536880105 peak = 1040502784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285777, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 307, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (333.35, 157.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676550 sequences (100000124 bp) in 2.736 CPU sec, 3.203 real sec...
[M::mem_process_seqs] Processed 677128 reads in 183.275 CPU sec, 6.048 real sec
[M::mem_process_seqs] Processed 678408 reads in 119.780 CPU sec, 3.933 real sec
sorting block 83, #reads = 1080207 mem = 536885208 peak = 1048182784
[M::process] read 676926 sequences (100000103 bp) in 2.546 CPU sec, 3.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284329, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (352.76, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285638, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676630 sequences (100000189 bp) in 2.444 CPU sec, 2.870 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (350.83, 164.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676550 reads in 185.286 CPU sec, 6.086 real sec
[M::mem_process_seqs] Processed 676926 reads in 119.713 CPU sec, 3.891 real sec
sorting block 84, #reads = 1080191 mem = 536878227 peak = 1048182784
[M::process] read 676118 sequences (100000090 bp) in 2.365 CPU sec, 2.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285055, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (352.24, 163.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675714 sequences (100000081 bp) in 2.566 CPU sec, 3.170 real sec...
sorting block 85, #reads = 1079658 mem = 536877655 peak = 1053310976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284609, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (351.76, 160.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676630 reads in 188.195 CPU sec, 6.135 real sec
[M::mem_process_seqs] Processed 676118 reads in 124.104 CPU sec, 4.024 real sec
[M::process] read 675514 sequences (100000153 bp) in 2.598 CPU sec, 2.912 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284821, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (353.70, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 86, #reads = 1077892 mem = 536877630 peak = 1053310976
[M::process] read 675336 sequences (100000138 bp) in 2.763 CPU sec, 3.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284401, 13, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (355.43, 160.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (452, 452, 1906)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4814)
[M::mem_pestat] mean and std.dev: (842.00, 729.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6268)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675714 reads in 187.350 CPU sec, 6.106 real sec
[M::mem_process_seqs] Processed 675514 reads in 125.567 CPU sec, 4.044 real sec
[M::process] read 674962 sequences (100000096 bp) in 2.446 CPU sec, 2.624 real sec...
sorting block 87, #reads = 1077366 mem = 536879626 peak = 1053310976
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285242, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (354.38, 159.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675066 sequences (100000026 bp) in 2.620 CPU sec, 3.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284580, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (357.78, 161.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675336 reads in 187.256 CPU sec, 6.146 real sec
[M::mem_process_seqs] Processed 674962 reads in 123.108 CPU sec, 3.997 real sec
[M::process] read 675232 sequences (100000013 bp) in 2.407 CPU sec, 2.642 real sec...
sorting block 88, #reads = 1077269 mem = 536881729 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284780, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (356.65, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674968 sequences (100000115 bp) in 2.704 CPU sec, 3.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284564, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (356.28, 161.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675066 reads in 187.970 CPU sec, 6.192 real sec
[M::mem_process_seqs] Processed 675232 reads in 124.590 CPU sec, 4.030 real sec
sorting block 89, #reads = 1077445 mem = 536881297 peak = 1083957248
[M::process] read 674842 sequences (100000230 bp) in 2.400 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284610, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (357.72, 162.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674870 sequences (100000116 bp) in 2.559 CPU sec, 2.978 real sec...
sorting block 90, #reads = 1075932 mem = 536878082 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284498, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (360.01, 164.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674968 reads in 182.512 CPU sec, 6.023 real sec
[M::mem_process_seqs] Processed 674842 reads in 122.728 CPU sec, 4.070 real sec
[M::process] read 680138 sequences (100000034 bp) in 2.835 CPU sec, 3.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285172, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (361.55, 165.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 91, #reads = 1076758 mem = 536879999 peak = 1083957248
[M::process] read 681634 sequences (100000155 bp) in 2.619 CPU sec, 2.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286824, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 298, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (325.16, 154.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674870 reads in 192.817 CPU sec, 6.251 real sec
[M::mem_process_seqs] Processed 680138 reads in 129.591 CPU sec, 4.172 real sec
[M::process] read 681752 sequences (100000255 bp) in 2.451 CPU sec, 2.682 real sec...
sorting block 92, #reads = 1076725 mem = 536879361 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287264, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 287, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (313.97, 148.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680522 sequences (100000104 bp) in 2.801 CPU sec, 3.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286843, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 286, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (309.85, 143.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681634 reads in 203.147 CPU sec, 6.571 real sec
[M::mem_process_seqs] Processed 681752 reads in 135.396 CPU sec, 4.310 real sec
[M::process] read 679888 sequences (100000086 bp) in 2.298 CPU sec, 2.355 real sec...
sorting block 93, #reads = 1077755 mem = 536882537 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287351, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 296, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (319.60, 148.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679282 sequences (100000190 bp) in 2.456 CPU sec, 2.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287010, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 300, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (323.30, 150.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678412 sequences (100000062 bp) in 1.951 CPU sec, 1.952 real sec...
[M::mem_process_seqs] Processed 680522 reads in 192.819 CPU sec, 6.165 real sec
sorting block 94, #reads = 1074349 mem = 536879627 peak = 1083957248
[M::mem_process_seqs] Processed 679888 reads in 125.416 CPU sec, 3.990 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286375, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 305, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (327.73, 152.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 95, #reads = 1076962 mem = 536883352 peak = 1083957248
[M::process] read 678364 sequences (100000112 bp) in 2.753 CPU sec, 2.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286584, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (334.88, 154.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678030 sequences (100000018 bp) in 1.968 CPU sec, 1.971 real sec...
[M::mem_process_seqs] Processed 679282 reads in 192.644 CPU sec, 6.160 real sec
[M::mem_process_seqs] Processed 678412 reads in 121.359 CPU sec, 3.843 real sec
sorting block 96, #reads = 1076451 mem = 536876617 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286331, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (337.01, 156.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677590 sequences (100000040 bp) in 2.737 CPU sec, 2.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286127, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (338.28, 156.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677614 sequences (100000246 bp) in 1.910 CPU sec, 1.916 real sec...
[M::mem_process_seqs] Processed 678364 reads in 176.206 CPU sec, 5.603 real sec
[M::mem_process_seqs] Processed 678030 reads in 112.483 CPU sec, 3.555 real sec
sorting block 97, #reads = 1078561 mem = 536877458 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286192, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (341.88, 157.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677596 sequences (100000187 bp) in 2.530 CPU sec, 2.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285823, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (339.70, 155.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677308 sequences (100000160 bp) in 1.886 CPU sec, 1.893 real sec...
[M::mem_process_seqs] Processed 677590 reads in 182.084 CPU sec, 5.797 real sec
[M::mem_process_seqs] Processed 677614 reads in 113.897 CPU sec, 3.613 real sec
sorting block 98, #reads = 1077411 mem = 536880091 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286172, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 321, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (340.80, 156.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677062 sequences (100000010 bp) in 2.773 CPU sec, 2.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285691, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (341.14, 156.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676828 sequences (100000042 bp) in 1.858 CPU sec, 1.863 real sec...
[M::mem_process_seqs] Processed 677596 reads in 173.385 CPU sec, 5.504 real sec
sorting block 99, #reads = 1075680 mem = 536883129 peak = 1083957248
[M::mem_process_seqs] Processed 677308 reads in 110.210 CPU sec, 3.483 real sec
sorting block 100, #reads = 1078526 mem = 536879824 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285558, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (345.79, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676878 sequences (100000213 bp) in 3.024 CPU sec, 3.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285962, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (347.84, 160.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676668 sequences (100000240 bp) in 1.622 CPU sec, 1.625 real sec...
[M::mem_process_seqs] Processed 677062 reads in 179.261 CPU sec, 5.704 real sec
[M::mem_process_seqs] Processed 676828 reads in 113.642 CPU sec, 3.596 real sec
sorting block 101, #reads = 1077044 mem = 536879063 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285237, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (345.02, 158.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675988 sequences (100000052 bp) in 2.768 CPU sec, 2.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284892, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (350.81, 161.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675346 sequences (100000072 bp) in 1.802 CPU sec, 1.808 real sec...
[M::mem_process_seqs] Processed 676878 reads in 170.500 CPU sec, 5.415 real sec
[M::mem_process_seqs] Processed 676668 reads in 109.614 CPU sec, 3.456 real sec
sorting block 102, #reads = 1077824 mem = 536877141 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284579, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (355.31, 162.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675276 sequences (100000060 bp) in 2.772 CPU sec, 2.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284175, 13, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 342, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (360.45, 164.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (385, 836, 846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1768)
[M::mem_pestat] mean and std.dev: (596.27, 311.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2229)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675442 sequences (100000243 bp) in 1.873 CPU sec, 1.875 real sec...
[M::mem_process_seqs] Processed 675988 reads in 170.658 CPU sec, 5.405 real sec
sorting block 103, #reads = 1078509 mem = 536882315 peak = 1083957248
[M::mem_process_seqs] Processed 675346 reads in 111.321 CPU sec, 3.517 real sec
sorting block 104, #reads = 1078278 mem = 536879231 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285025, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (359.18, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675430 sequences (100000137 bp) in 2.662 CPU sec, 2.750 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284729, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (358.16, 164.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675418 sequences (100000232 bp) in 1.873 CPU sec, 1.874 real sec...
[M::mem_process_seqs] Processed 675276 reads in 164.835 CPU sec, 5.221 real sec
[M::mem_process_seqs] Processed 675442 reads in 104.792 CPU sec, 3.310 real sec
sorting block 105, #reads = 1078100 mem = 536882568 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284833, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (359.47, 167.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675940 sequences (100000032 bp) in 2.803 CPU sec, 2.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285849, 11, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 338, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (359.73, 166.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (575, 1075, 1978)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4784)
[M::mem_pestat] mean and std.dev: (1405.27, 1229.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6322)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675430 reads in 167.455 CPU sec, 5.315 real sec
[M::process] read 676082 sequences (100000061 bp) in 1.888 CPU sec, 1.890 real sec...
[M::mem_process_seqs] Processed 675418 reads in 110.278 CPU sec, 3.478 real sec
sorting block 106, #reads = 1077814 mem = 536886517 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285475, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (355.26, 166.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677802 sequences (100000113 bp) in 2.840 CPU sec, 2.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285182, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (357.73, 168.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680430 sequences (100000004 bp) in 1.798 CPU sec, 1.800 real sec...
[M::mem_process_seqs] Processed 675940 reads in 169.645 CPU sec, 5.376 real sec
[M::mem_process_seqs] Processed 676082 reads in 113.582 CPU sec, 3.589 real sec
sorting block 107, #reads = 1078080 mem = 536884174 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285180, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 318, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (342.87, 162.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680114 sequences (100000172 bp) in 2.816 CPU sec, 2.903 real sec...
[M::process] read 679330 sequences (100000298 bp) in 1.760 CPU sec, 1.766 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286689, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 296, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (322.64, 153.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677802 reads in 178.671 CPU sec, 5.666 real sec
sorting block 108, #reads = 1074396 mem = 536879380 peak = 1083957248
[M::mem_process_seqs] Processed 680430 reads in 106.044 CPU sec, 3.355 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286439, 3, 21)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 297, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (322.88, 152.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 253, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (303.10, 179.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RR
sorting block 109, #reads = 1074496 mem = 536883130 peak = 1083957248
[M::process] read 678428 sequences (100000246 bp) in 2.928 CPU sec, 3.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285768, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (330.67, 156.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678222 sequences (100000135 bp) in 1.450 CPU sec, 1.460 real sec...
[M::mem_process_seqs] Processed 680114 reads in 162.642 CPU sec, 5.167 real sec
[M::mem_process_seqs] Processed 679330 reads in 102.589 CPU sec, 3.247 real sec
sorting block 110, #reads = 1080394 mem = 536880632 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286088, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 310, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (333.87, 155.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678192 sequences (100000246 bp) in 2.794 CPU sec, 2.898 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285660, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (336.17, 157.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677532 sequences (100000154 bp) in 1.820 CPU sec, 1.818 real sec...
[M::mem_process_seqs] Processed 678428 reads in 159.081 CPU sec, 5.035 real sec
[M::mem_process_seqs] Processed 678222 reads in 102.471 CPU sec, 3.238 real sec
sorting block 111, #reads = 1081211 mem = 536877588 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286325, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (337.43, 157.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677428 sequences (100000237 bp) in 2.550 CPU sec, 2.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285986, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (341.34, 158.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677268 sequences (100000139 bp) in 1.880 CPU sec, 1.873 real sec...
[M::mem_process_seqs] Processed 678192 reads in 161.841 CPU sec, 5.131 real sec
[M::mem_process_seqs] Processed 677532 reads in 104.395 CPU sec, 3.286 real sec
sorting block 112, #reads = 1081229 mem = 536878862 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285755, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (343.20, 159.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 307, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (229.44, 79.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RR
[M::process] read 677094 sequences (100000185 bp) in 2.607 CPU sec, 2.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286172, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (344.90, 159.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676994 sequences (100000062 bp) in 1.846 CPU sec, 1.849 real sec...
[M::mem_process_seqs] Processed 677428 reads in 161.035 CPU sec, 5.108 real sec
sorting block 113, #reads = 1080792 mem = 536878554 peak = 1083957248
[M::mem_process_seqs] Processed 677268 reads in 103.924 CPU sec, 3.276 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285241, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (344.55, 159.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 114, #reads = 1080327 mem = 536878979 peak = 1083957248
[M::process] read 676960 sequences (100000103 bp) in 2.534 CPU sec, 2.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285758, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (346.73, 160.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677094 reads in 160.834 CPU sec, 5.110 real sec
[M::process] read 676680 sequences (100000095 bp) in 1.895 CPU sec, 1.898 real sec...
[M::mem_process_seqs] Processed 676994 reads in 103.845 CPU sec, 3.274 real sec
sorting block 115, #reads = 1080190 mem = 536879513 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285013, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (347.36, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676472 sequences (100000279 bp) in 2.802 CPU sec, 2.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285532, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (349.07, 161.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676960 reads in 159.847 CPU sec, 5.060 real sec
[M::process] read 676224 sequences (100000171 bp) in 1.840 CPU sec, 1.839 real sec...
[M::mem_process_seqs] Processed 676680 reads in 103.857 CPU sec, 3.276 real sec
sorting block 116, #reads = 1079016 mem = 536880104 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285409, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (352.61, 162.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676268 sequences (100000232 bp) in 2.722 CPU sec, 2.819 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285359, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (352.88, 163.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676110 sequences (100000033 bp) in 1.810 CPU sec, 1.816 real sec...
[M::mem_process_seqs] Processed 676472 reads in 161.515 CPU sec, 5.114 real sec
[M::mem_process_seqs] Processed 676224 reads in 103.647 CPU sec, 3.275 real sec
sorting block 117, #reads = 1079791 mem = 536879525 peak = 1083957248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285680, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (353.05, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676084 sequences (100000267 bp) in 3.016 CPU sec, 3.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284996, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (352.01, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676268 reads in 166.935 CPU sec, 5.294 real sec
[M::process] read 675878 sequences (100000130 bp) in 1.850 CPU sec, 1.855 real sec...
sorting block 118, #reads = 1078854 mem = 536880218 peak = 1083957248
[M::mem_process_seqs] Processed 676110 reads in 109.352 CPU sec, 3.447 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285254, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (349.34, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 170, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (206.30, 85.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] skip orientation RR
sorting block 119, #reads = 1078993 mem = 536880216 peak = 1086607360
[M::process] read 676150 sequences (100000212 bp) in 2.846 CPU sec, 2.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284823, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (350.53, 159.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676316 sequences (100000162 bp) in 1.818 CPU sec, 1.822 real sec...
[M::mem_process_seqs] Processed 676084 reads in 166.209 CPU sec, 5.273 real sec
[M::mem_process_seqs] Processed 675878 reads in 106.457 CPU sec, 3.364 real sec
sorting block 120, #reads = 1078460 mem = 536878483 peak = 1086607360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284901, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (483, 1188, 3341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9057)
[M::mem_pestat] mean and std.dev: (1587.73, 1407.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11915)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (349.92, 159.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (397, 610, 2330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6196)
[M::mem_pestat] mean and std.dev: (968.18, 843.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8129)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 676148 sequences (100000100 bp) in 2.759 CPU sec, 2.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285289, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (348.69, 159.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676358 sequences (100000174 bp) in 1.856 CPU sec, 1.857 real sec...
[M::mem_process_seqs] Processed 676150 reads in 168.787 CPU sec, 5.357 real sec
[M::mem_process_seqs] Processed 676316 reads in 110.806 CPU sec, 3.499 real sec
sorting block 121, #reads = 1078356 mem = 536880039 peak = 1086607360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284903, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (349.24, 160.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676464 sequences (100000187 bp) in 2.515 CPU sec, 2.598 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285567, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (350.29, 161.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676006 sequences (100000126 bp) in 1.860 CPU sec, 1.857 real sec...
[M::mem_process_seqs] Processed 676148 reads in 165.712 CPU sec, 5.266 real sec
sorting block 122, #reads = 1077864 mem = 536878483 peak = 1086607360
[M::mem_process_seqs] Processed 676358 reads in 106.061 CPU sec, 3.351 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285104, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.76, 162.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676346 sequences (100000177 bp) in 2.861 CPU sec, 2.959 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284961, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (352.62, 163.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676318 sequences (100000267 bp) in 1.920 CPU sec, 1.923 real sec...
[M::mem_process_seqs] Processed 676464 reads in 169.813 CPU sec, 5.382 real sec
sorting block 123, #reads = 1077812 mem = 536878913 peak = 1086607360
[M::mem_process_seqs] Processed 676006 reads in 109.553 CPU sec, 3.462 real sec
sorting block 124, #reads = 1076998 mem = 536878642 peak = 1127956480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285292, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (351.98, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683002 sequences (100000054 bp) in 3.062 CPU sec, 3.172 real sec...
[M::process] read 682532 sequences (100000273 bp) in 1.930 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284605, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (353.16, 165.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676346 reads in 191.487 CPU sec, 6.074 real sec
[M::mem_process_seqs] Processed 676318 reads in 129.236 CPU sec, 4.091 real sec
sorting block 125, #reads = 1072780 mem = 536878967 peak = 1127956480
[M::process] read 681510 sequences (100000080 bp) in 2.500 CPU sec, 2.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287715, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 278, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (305.63, 145.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680488 sequences (100000096 bp) in 1.914 CPU sec, 1.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287695, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 283, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (309.88, 147.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287568, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 289, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (315.02, 148.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683002 reads in 263.595 CPU sec, 8.405 real sec
[M::mem_process_seqs] Processed 682532 reads in 196.613 CPU sec, 6.288 real sec
sorting block 126, #reads = 1069290 mem = 536878317 peak = 1127956480
[M::process] read 679740 sequences (100000284 bp) in 2.787 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287140, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 297, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (320.82, 150.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680488 reads in 102.537 CPU sec, 3.443 real sec
sorting block 127, #reads = 1075413 mem = 536880017 peak = 1127956480
[M::mem_process_seqs] Processed 681510 reads in 370.790 CPU sec, 12.029 real sec
[M::process] read 679084 sequences (100000101 bp) in 2.487 CPU sec, 2.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286680, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 302, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (325.44, 151.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 128, #reads = 1074886 mem = 536879788 peak = 1127956480
[M::process] read 678488 sequences (100000017 bp) in 2.435 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286303, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (967, 3471, 3471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8479)
[M::mem_pestat] mean and std.dev: (2648.10, 1270.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10983)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 307, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (329.73, 153.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 679740 reads in 196.176 CPU sec, 6.185 real sec
[M::mem_process_seqs] Processed 679084 reads in 112.566 CPU sec, 3.544 real sec
[M::process] read 678060 sequences (100000238 bp) in 2.226 CPU sec, 2.281 real sec...
sorting block 129, #reads = 1075147 mem = 536879213 peak = 1127956480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285571, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (334.32, 154.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677926 sequences (100000036 bp) in 2.623 CPU sec, 2.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285718, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (2492, 2492, 2492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2492, 2492)
[M::mem_pestat] mean and std.dev: (2492.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (2492, 2492)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (337.62, 155.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 678488 reads in 171.057 CPU sec, 5.436 real sec
[M::process] read 677376 sequences (100000301 bp) in 1.923 CPU sec, 1.935 real sec...
[M::mem_process_seqs] Processed 678060 reads in 114.535 CPU sec, 3.616 real sec
sorting block 130, #reads = 1076440 mem = 536881363 peak = 1127956480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286121, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (340.22, 157.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (370, 448, 1373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3379)
[M::mem_pestat] mean and std.dev: (691.00, 434.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4382)
[M::mem_pestat] skip orientation RR
[M::process] read 677340 sequences (100000075 bp) in 2.740 CPU sec, 2.844 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285535, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 438, 619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427)
[M::mem_pestat] mean and std.dev: (436.09, 259.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1831)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (344.57, 158.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (345, 345, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (345, 345)
[M::mem_pestat] mean and std.dev: (345.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (345, 345)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 677326 sequences (100000107 bp) in 1.900 CPU sec, 1.906 real sec...
[M::mem_process_seqs] Processed 677926 reads in 170.091 CPU sec, 5.400 real sec
[M::mem_process_seqs] Processed 677376 reads in 117.407 CPU sec, 3.734 real sec
sorting block 131, #reads = 1074840 mem = 536878664 peak = 1127956480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286322, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 326, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (346.46, 160.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676904 sequences (100000087 bp) in 2.503 CPU sec, 2.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285356, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (346.06, 160.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677214 sequences (100000169 bp) in 1.890 CPU sec, 1.896 real sec...
[M::mem_process_seqs] Processed 677340 reads in 171.346 CPU sec, 5.437 real sec
sorting block 132, #reads = 1076909 mem = 536880328 peak = 1127956480
[M::mem_process_seqs] Processed 677326 reads in 111.348 CPU sec, 3.522 real sec
sorting block 133, #reads = 1077003 mem = 536879389 peak = 1127956480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285774, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (346.99, 160.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676992 sequences (100000121 bp) in 2.394 CPU sec, 2.518 real sec...
[M::process] read 676914 sequences (100000052 bp) in 1.420 CPU sec, 1.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286176, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (343.29, 158.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676904 reads in 172.401 CPU sec, 5.490 real sec
[M::mem_process_seqs] Processed 677214 reads in 109.497 CPU sec, 3.482 real sec
sorting block 134, #reads = 1076497 mem = 536880773 peak = 1127956480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285122, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (346.56, 160.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677136 sequences (100000231 bp) in 2.822 CPU sec, 2.980 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285569, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 223, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338)
[M::mem_pestat] mean and std.dev: (286.80, 172.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1736)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (346.70, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676802 sequences (100000016 bp) in 1.860 CPU sec, 1.860 real sec...
[M::mem_process_seqs] Processed 676992 reads in 169.075 CPU sec, 5.355 real sec
[M::mem_process_seqs] Processed 676914 reads in 108.461 CPU sec, 3.425 real sec
sorting block 135, #reads = 1076703 mem = 536880237 peak = 1127956480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285170, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (345.18, 161.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (408, 408, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (116, 846)
[M::mem_pestat] mean and std.dev: (412.45, 77.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RR
[M::process] read 676872 sequences (100000076 bp) in 2.745 CPU sec, 2.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285357, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (348.14, 162.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676588 sequences (100000189 bp) in 1.845 CPU sec, 1.852 real sec...
[M::mem_process_seqs] Processed 677136 reads in 169.718 CPU sec, 5.381 real sec
[M::mem_process_seqs] Processed 676802 reads in 109.329 CPU sec, 3.451 real sec
sorting block 136, #reads = 1077296 mem = 536878817 peak = 1127956480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285163, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (278, 973, 993)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2423)
[M::mem_pestat] mean and std.dev: (624.10, 382.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3138)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (348.43, 162.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676398 sequences (100000070 bp) in 2.524 CPU sec, 2.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285268, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (347.28, 161.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676364 sequences (100000260 bp) in 1.880 CPU sec, 1.882 real sec...
[M::mem_process_seqs] Processed 676872 reads in 169.607 CPU sec, 5.385 real sec
sorting block 137, #reads = 1078580 mem = 536880496 peak = 1127956480
[M::mem_process_seqs] Processed 676588 reads in 109.404 CPU sec, 3.451 real sec
sorting block 138, #reads = 1079408 mem = 536880286 peak = 1127956480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284931, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (349.31, 162.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676090 sequences (100000026 bp) in 2.498 CPU sec, 2.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285292, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (352.10, 164.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675758 sequences (100000106 bp) in 1.810 CPU sec, 1.807 real sec...
[M::mem_process_seqs] Processed 676398 reads in 158.842 CPU sec, 5.035 real sec
[M::mem_process_seqs] Processed 676364 reads in 100.888 CPU sec, 3.179 real sec
sorting block 139, #reads = 1079059 mem = 536878802 peak = 1127956480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285396, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (354.73, 166.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676004 sequences (100000098 bp) in 2.740 CPU sec, 2.835 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284828, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (355.55, 166.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 554888 sequences (82051507 bp) in 1.640 CPU sec, 1.638 real sec...
[M::mem_process_seqs] Processed 676090 reads in 158.813 CPU sec, 5.030 real sec
[M::mem_process_seqs] Processed 675758 reads in 104.123 CPU sec, 3.286 real sec
sorting block 140, #reads = 1079523 mem = 536880844 peak = 1127956480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285263, 6, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (357.18, 168.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 407, 1505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4187)
[M::mem_pestat] mean and std.dev: (904.12, 1187.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5653)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 233860, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (354.70, 167.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676004 reads in 148.071 CPU sec, 4.795 real sec
[M::mem_process_seqs] Processed 554888 reads in 89.783 CPU sec, 2.903 real sec
sorting block 141, #reads = 1079343 mem = 536878248 peak = 1171402752
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 153469734
overall: 69963419648 mem 22361.432 user 496.355 sys 750.181 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_d0M-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tLB:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 750.227 sec; CPU: 22857.797 sec
sorting block 142, #reads = 866402 mem = 431754895 peak = 1208332288
uniq header count 1
merging 143 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8213448619
output reads: 154219747
bam_mem_sort: 143 calls 91.580 user 1.386 sys 95.097 real
bam_write: 143 calls 63.453 user 4.844 sys 105.092 real
execute: 1 calls 46.326 user 1.674 sys 803.947 real
merge_files: 1 calls 46.302 user 1.653 sys 53.186 real
parse_chunk: 2285 calls 100.817 user 28.192 sys 147.183 real
read_chunk: 6156 calls 50.073 user 42.967 sys 702.531 real
sort_block: 1 calls 33.304 user 42.221 sys 678.534 real
write_chunk: 3869 calls 45.939 user 1.384 sys 52.298 real
overall: 3256561664 mem 2106.662 user 99.613 sys 803.967 real
ls: cannot access '*.cram': No such file or directory