cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 2089.601 CPU sec, 312.902 real sec
[M::process] read 702858 sequences (100000258 bp) in 0.975 CPU sec, 0.980 real sec...
[M::process] read 701750 sequences (100000058 bp) in 1.309 CPU sec, 1.315 real sec...
[M::process] read 700662 sequences (100000104 bp) in 1.538 CPU sec, 1.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291963, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.66, 114.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699804 sequences (100000179 bp) in 1.427 CPU sec, 1.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292211, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.79, 116.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291803, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.69, 117.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702858 reads in 263.527 CPU sec, 8.448 real sec
[M::mem_process_seqs] Processed 701750 reads in 156.334 CPU sec, 5.098 real sec
[M::mem_process_seqs] Processed 700662 reads in 170.232 CPU sec, 5.586 real sec
sorting block 0, #reads = 1096033 mem = 536881577 peak = 597024768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291697, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 224, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593)
[M::mem_pestat] mean and std.dev: (218.89, 66.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.16, 117.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699804 reads in 82.993 CPU sec, 2.758 real sec
[M::process] read 698992 sequences (100000169 bp) in 2.662 CPU sec, 3.031 real sec...
sorting block 1, #reads = 1096486 mem = 536879523 peak = 810934272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291208, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.55, 119.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 187, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528)
[M::mem_pestat] mean and std.dev: (199.60, 59.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 647)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698992 reads in 82.900 CPU sec, 2.724 real sec
[M::process] read 698396 sequences (100000001 bp) in 2.572 CPU sec, 2.928 real sec...
sorting block 2, #reads = 1094350 mem = 536877272 peak = 838397952
[M::process] read 698200 sequences (100000186 bp) in 1.943 CPU sec, 2.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291024, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.85, 121.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 307, 2554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7200)
[M::mem_pestat] mean and std.dev: (1048.83, 1325.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9523)
[M::mem_pestat] skip orientation RR
[M::process] read 697596 sequences (100000107 bp) in 1.380 CPU sec, 1.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290835, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.12, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 340, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (392.09, 196.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698396 reads in 149.155 CPU sec, 4.707 real sec
[M::mem_process_seqs] Processed 698200 reads in 96.205 CPU sec, 3.045 real sec
[M::process] read 697938 sequences (100000064 bp) in 1.638 CPU sec, 1.687 real sec...
sorting block 3, #reads = 1093057 mem = 536878755 peak = 838397952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291033, 12, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 426, 912)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2350)
[M::mem_pestat] mean and std.dev: (623.20, 509.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3069)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.66, 120.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 251, 2060)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6088)
[M::mem_pestat] mean and std.dev: (712.17, 885.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8102)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 697070 sequences (100000265 bp) in 2.841 CPU sec, 3.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291138, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.07, 119.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (406, 586, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (46, 946)
[M::mem_pestat] mean and std.dev: (452.44, 164.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697596 reads in 146.965 CPU sec, 4.720 real sec
[M::mem_process_seqs] Processed 697938 reads in 96.493 CPU sec, 3.086 real sec
sorting block 4, #reads = 1093710 mem = 536880114 peak = 838397952
[M::process] read 696414 sequences (100000281 bp) in 2.485 CPU sec, 2.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290433, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.55, 121.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696224 sequences (100000165 bp) in 2.253 CPU sec, 2.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290407, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.43, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697070 reads in 143.967 CPU sec, 4.611 real sec
[M::mem_process_seqs] Processed 696414 reads in 89.772 CPU sec, 2.845 real sec
sorting block 5, #reads = 1093599 mem = 536878143 peak = 838397952
[M::process] read 696200 sequences (100000089 bp) in 2.504 CPU sec, 2.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289996, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.87, 118.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290004, 4, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (298, 473, 718)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558)
[M::mem_pestat] mean and std.dev: (584.14, 393.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2160)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.44, 119.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696224 reads in 143.947 CPU sec, 4.634 real sec
sorting block 6, #reads = 1092801 mem = 536880011 peak = 838397952
[M::mem_process_seqs] Processed 696200 reads in 91.228 CPU sec, 2.918 real sec
[M::process] read 696972 sequences (100000128 bp) in 3.300 CPU sec, 3.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290645, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (323, 1209, 2804)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7766)
[M::mem_pestat] mean and std.dev: (1285.38, 1067.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10247)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.42, 118.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696972 reads in 79.579 CPU sec, 2.639 real sec
sorting block 7, #reads = 1091467 mem = 536879302 peak = 838397952
[M::process] read 696900 sequences (100000080 bp) in 3.260 CPU sec, 3.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290292, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.39, 118.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696900 reads in 79.541 CPU sec, 2.613 real sec
[M::process] read 696812 sequences (100000094 bp) in 3.224 CPU sec, 3.628 real sec...
sorting block 8, #reads = 1091238 mem = 536878372 peak = 838397952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290344, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.45, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696812 reads in 80.855 CPU sec, 2.624 real sec
[M::process] read 696302 sequences (100000251 bp) in 3.356 CPU sec, 3.779 real sec...
sorting block 9, #reads = 1091590 mem = 536883054 peak = 838397952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290144, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.35, 120.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696302 reads in 81.112 CPU sec, 2.625 real sec
[M::process] read 696690 sequences (100000112 bp) in 2.814 CPU sec, 3.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290319, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.43, 120.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696690 reads in 81.345 CPU sec, 2.638 real sec
sorting block 10, #reads = 1091454 mem = 536879954 peak = 838397952
[M::process] read 702500 sequences (100000037 bp) in 3.255 CPU sec, 3.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292340, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.69, 115.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700780 sequences (100000066 bp) in 2.324 CPU sec, 2.523 real sec...
[M::mem_process_seqs] Processed 702500 reads in 80.316 CPU sec, 2.594 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291574, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (243.81, 115.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 11, #reads = 1094067 mem = 536881659 peak = 838397952
[M::mem_process_seqs] Processed 700780 reads in 78.454 CPU sec, 2.540 real sec
[M::process] read 699706 sequences (100000032 bp) in 3.063 CPU sec, 3.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291164, 3, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.22, 116.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 365, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243)
[M::mem_pestat] mean and std.dev: (444.75, 327.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1753)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699706 reads in 79.232 CPU sec, 2.572 real sec
sorting block 12, #reads = 1096567 mem = 536878059 peak = 838397952
[M::process] read 699292 sequences (100000013 bp) in 3.124 CPU sec, 3.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291366, 7, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 216, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433)
[M::mem_pestat] mean and std.dev: (291.25, 222.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1852)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (247.97, 117.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699292 reads in 78.945 CPU sec, 2.556 real sec
[M::process] read 699118 sequences (100000287 bp) in 2.664 CPU sec, 2.908 real sec...
sorting block 13, #reads = 1095891 mem = 536880907 peak = 838397952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290935, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (248.95, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699118 reads in 78.259 CPU sec, 2.538 real sec
[M::process] read 698756 sequences (100000164 bp) in 2.884 CPU sec, 3.166 real sec...
[M::process] read 698036 sequences (100000300 bp) in 1.957 CPU sec, 2.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291152, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.57, 119.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697818 sequences (100000257 bp) in 1.450 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290687, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.08, 119.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698756 reads in 142.235 CPU sec, 4.482 real sec
[M::mem_process_seqs] Processed 698036 reads in 88.788 CPU sec, 2.796 real sec
sorting block 14, #reads = 1093979 mem = 536880656 peak = 838397952
[M::process] read 697572 sequences (100000256 bp) in 2.124 CPU sec, 2.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290370, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.79, 120.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 322, 2730)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7846)
[M::mem_pestat] mean and std.dev: (1108.00, 1187.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10404)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290934, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.04, 121.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 241, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (270.00, 150.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697818 reads in 142.263 CPU sec, 4.591 real sec
sorting block 15, #reads = 1095259 mem = 536878672 peak = 839782400
[M::mem_process_seqs] Processed 697572 reads in 90.365 CPU sec, 2.888 real sec
[M::process] read 696768 sequences (100000043 bp) in 3.302 CPU sec, 3.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290445, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.15, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696768 reads in 75.700 CPU sec, 2.508 real sec
sorting block 16, #reads = 1093740 mem = 536878449 peak = 847220736
[M::process] read 697094 sequences (100000062 bp) in 2.765 CPU sec, 3.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290357, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (253.85, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697094 reads in 77.056 CPU sec, 2.532 real sec
[M::process] read 696624 sequences (100000266 bp) in 3.174 CPU sec, 3.637 real sec...
sorting block 17, #reads = 1092986 mem = 536877985 peak = 847220736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290003, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.48, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696624 reads in 77.347 CPU sec, 2.518 real sec
[M::process] read 697754 sequences (100000181 bp) in 3.165 CPU sec, 3.552 real sec...
sorting block 18, #reads = 1092595 mem = 536879712 peak = 847220736
[M::process] read 696872 sequences (100000298 bp) in 2.014 CPU sec, 2.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290911, 11, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.73, 118.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (93, 119, 1687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4875)
[M::mem_pestat] mean and std.dev: (907.60, 1408.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6541)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 696710 sequences (100000257 bp) in 1.510 CPU sec, 1.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290586, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 166, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (171.56, 71.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.81, 118.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697754 reads in 143.264 CPU sec, 4.543 real sec
[M::mem_process_seqs] Processed 696872 reads in 88.578 CPU sec, 2.793 real sec
[M::process] read 696710 sequences (100000271 bp) in 2.325 CPU sec, 2.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290384, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.92, 118.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1093283 mem = 536878396 peak = 847220736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290346, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.66, 118.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (380, 460, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337)
[M::mem_pestat] mean and std.dev: (493.75, 160.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1656)
[M::mem_pestat] skip orientation RR
[M::process] read 696796 sequences (100000263 bp) in 2.313 CPU sec, 2.448 real sec...
[M::mem_process_seqs] Processed 696710 reads in 143.960 CPU sec, 4.636 real sec
[M::mem_process_seqs] Processed 696710 reads in 89.878 CPU sec, 2.850 real sec
sorting block 20, #reads = 1092964 mem = 536879191 peak = 861229056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290226, 10, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.98, 120.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (378, 1372, 1821)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4707)
[M::mem_pestat] mean and std.dev: (1192.20, 757.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6150)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 696758 sequences (100000188 bp) in 2.720 CPU sec, 2.988 real sec...
[M::mem_process_seqs] Processed 696796 reads in 79.549 CPU sec, 2.631 real sec
sorting block 21, #reads = 1092818 mem = 536877571 peak = 861229056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290244, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.17, 121.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701412 sequences (100000159 bp) in 2.589 CPU sec, 2.761 real sec...
[M::mem_process_seqs] Processed 696758 reads in 81.166 CPU sec, 2.662 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291812, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (241.73, 114.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701412 reads in 79.371 CPU sec, 2.631 real sec
[M::process] read 700730 sequences (100000215 bp) in 3.182 CPU sec, 3.665 real sec...
sorting block 22, #reads = 1090962 mem = 536879300 peak = 861229056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291723, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 199, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (204.92, 77.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.15, 115.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700730 reads in 77.648 CPU sec, 2.559 real sec
[M::process] read 699234 sequences (100000040 bp) in 3.146 CPU sec, 3.538 real sec...
sorting block 23, #reads = 1095239 mem = 536878136 peak = 861229056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291041, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.08, 116.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699234 reads in 79.530 CPU sec, 2.606 real sec
[M::process] read 698794 sequences (100000126 bp) in 3.248 CPU sec, 3.809 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291443, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (248.62, 117.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 707, 934)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2542)
[M::mem_pestat] mean and std.dev: (419.00, 326.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3346)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698794 reads in 79.582 CPU sec, 2.585 real sec
sorting block 24, #reads = 1094647 mem = 536878892 peak = 861229056
[M::process] read 698580 sequences (100000134 bp) in 3.258 CPU sec, 3.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291110, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.73, 119.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698580 reads in 79.968 CPU sec, 2.598 real sec
[M::process] read 698576 sequences (100000148 bp) in 3.284 CPU sec, 3.779 real sec...
sorting block 25, #reads = 1095127 mem = 536877846 peak = 861229056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291242, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.00, 121.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698576 reads in 79.727 CPU sec, 2.595 real sec
[M::process] read 697630 sequences (100000057 bp) in 3.214 CPU sec, 3.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291098, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 283, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (265.89, 110.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (252.87, 120.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697234 sequences (100000031 bp) in 2.319 CPU sec, 2.498 real sec...
[M::mem_process_seqs] Processed 697630 reads in 78.402 CPU sec, 2.539 real sec
sorting block 26, #reads = 1094671 mem = 536880288 peak = 861229056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290644, 6, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.23, 120.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 333, 558)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292)
[M::mem_pestat] mean and std.dev: (308.08, 141.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1659)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697234 reads in 77.314 CPU sec, 2.502 real sec
[M::process] read 697002 sequences (100000155 bp) in 2.993 CPU sec, 3.326 real sec...
sorting block 27, #reads = 1093554 mem = 536877230 peak = 865722368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290650, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.04, 120.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697002 reads in 78.329 CPU sec, 2.540 real sec
[M::process] read 696304 sequences (100000130 bp) in 3.185 CPU sec, 3.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290388, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (254.75, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696304 reads in 78.259 CPU sec, 2.537 real sec
sorting block 28, #reads = 1092733 mem = 536881579 peak = 876724224
[M::process] read 696342 sequences (100000215 bp) in 2.865 CPU sec, 3.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290281, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.69, 118.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (284, 291, 955)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2297)
[M::mem_pestat] mean and std.dev: (453.08, 424.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2968)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696342 reads in 79.073 CPU sec, 2.568 real sec
[M::process] read 696414 sequences (100000044 bp) in 3.003 CPU sec, 3.574 real sec...
sorting block 29, #reads = 1091994 mem = 536882438 peak = 876724224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290411, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (251.73, 118.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696414 reads in 78.877 CPU sec, 2.570 real sec
[M::process] read 696884 sequences (100000107 bp) in 2.678 CPU sec, 3.000 real sec...
[M::process] read 696656 sequences (100000013 bp) in 1.803 CPU sec, 1.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290041, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.07, 118.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 389, 8765)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25929)
[M::mem_pestat] mean and std.dev: (2579.08, 3920.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 34511)
[M::mem_pestat] skip orientation RR
[M::process] read 696942 sequences (100000301 bp) in 1.440 CPU sec, 1.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289952, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 265, 1621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4537)
[M::mem_pestat] mean and std.dev: (586.00, 638.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5995)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (250.58, 117.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 456, 755)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1949)
[M::mem_pestat] mean and std.dev: (502.80, 321.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2546)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696884 reads in 144.103 CPU sec, 4.540 real sec
[M::mem_process_seqs] Processed 696656 reads in 90.308 CPU sec, 2.846 real sec
[M::process] read 696892 sequences (100000185 bp) in 1.783 CPU sec, 1.869 real sec...
sorting block 30, #reads = 1092533 mem = 536886684 peak = 876724224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290590, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (250.08, 117.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1091221 mem = 536877036 peak = 948981760
[M::process] read 696626 sequences (100000296 bp) in 2.041 CPU sec, 2.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290443, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (250.71, 117.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696942 reads in 142.057 CPU sec, 4.547 real sec
[M::mem_process_seqs] Processed 696892 reads in 89.826 CPU sec, 2.842 real sec
[M::process] read 696640 sequences (100000112 bp) in 2.078 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290766, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.15, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 32, #reads = 1092307 mem = 536880179 peak = 948981760
[M::process] read 700060 sequences (100000082 bp) in 2.462 CPU sec, 2.774 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290456, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 203, 1053)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2777)
[M::mem_pestat] mean and std.dev: (547.82, 523.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3639)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.94, 121.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696626 reads in 137.674 CPU sec, 4.401 real sec
[M::mem_process_seqs] Processed 696640 reads in 87.358 CPU sec, 2.761 real sec
[M::process] read 700922 sequences (100000055 bp) in 2.742 CPU sec, 2.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291182, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.31, 117.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1091480 mem = 536877431 peak = 948981760
[M::mem_process_seqs] Processed 700060 reads in 76.931 CPU sec, 2.568 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291282, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (244.43, 116.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700922 reads in 74.592 CPU sec, 2.487 real sec
[M::process] read 699996 sequences (100000240 bp) in 3.137 CPU sec, 3.625 real sec...
sorting block 34, #reads = 1092294 mem = 536882277 peak = 948981760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291553, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 167, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (205.92, 118.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.47, 118.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699996 reads in 75.829 CPU sec, 2.516 real sec
[M::process] read 698718 sequences (100000070 bp) in 3.323 CPU sec, 3.822 real sec...
sorting block 35, #reads = 1095544 mem = 536881511 peak = 948981760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291386, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.17, 118.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698718 reads in 74.421 CPU sec, 2.450 real sec
[M::process] read 698588 sequences (100000224 bp) in 3.341 CPU sec, 3.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291002, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.80, 119.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1095300 mem = 536879281 peak = 948981760
[M::mem_process_seqs] Processed 698588 reads in 75.266 CPU sec, 2.438 real sec
[M::process] read 698384 sequences (100000048 bp) in 3.144 CPU sec, 3.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290757, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 238, 771)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2049)
[M::mem_pestat] mean and std.dev: (308.78, 256.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2688)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.00, 121.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 449, 849)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2225)
[M::mem_pestat] mean and std.dev: (549.47, 427.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2913)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698384 reads in 75.244 CPU sec, 2.440 real sec
sorting block 37, #reads = 1094685 mem = 536880856 peak = 948981760
[M::process] read 698172 sequences (100000117 bp) in 3.389 CPU sec, 3.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290754, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.12, 122.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698172 reads in 74.989 CPU sec, 2.422 real sec
[M::process] read 697682 sequences (100000018 bp) in 2.604 CPU sec, 2.897 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290600, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.76, 121.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1094155 mem = 536883267 peak = 948981760
[M::mem_process_seqs] Processed 697682 reads in 74.205 CPU sec, 2.401 real sec
[M::process] read 697416 sequences (100000299 bp) in 3.264 CPU sec, 3.768 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290685, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.30, 122.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697416 reads in 74.775 CPU sec, 2.414 real sec
[M::process] read 696082 sequences (100000283 bp) in 2.442 CPU sec, 2.983 real sec...
sorting block 39, #reads = 1093434 mem = 536880053 peak = 948981760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289757, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.14, 120.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 763, 1430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3838)
[M::mem_pestat] mean and std.dev: (766.75, 615.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5042)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696082 reads in 75.396 CPU sec, 2.439 real sec
[M::process] read 696512 sequences (100000118 bp) in 3.232 CPU sec, 3.730 real sec...
sorting block 40, #reads = 1092244 mem = 536878267 peak = 948981760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290503, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.27, 120.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696512 reads in 75.244 CPU sec, 2.427 real sec
[M::process] read 697414 sequences (100000291 bp) in 2.193 CPU sec, 2.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290332, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (248.67, 116.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697414 reads in 76.300 CPU sec, 2.466 real sec
[M::process] read 697292 sequences (100000025 bp) in 2.847 CPU sec, 3.164 real sec...
sorting block 41, #reads = 1091583 mem = 536879400 peak = 948981760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290380, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (249.71, 117.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697292 reads in 75.444 CPU sec, 2.454 real sec
[M::process] read 696822 sequences (100000012 bp) in 2.842 CPU sec, 3.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290417, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (250.60, 117.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696822 reads in 75.636 CPU sec, 2.459 real sec
sorting block 42, #reads = 1092362 mem = 536881320 peak = 948981760
[M::process] read 696970 sequences (100000234 bp) in 3.204 CPU sec, 3.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290596, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.37, 117.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696970 reads in 75.549 CPU sec, 2.450 real sec
[M::process] read 696860 sequences (100000009 bp) in 2.169 CPU sec, 2.473 real sec...
sorting block 43, #reads = 1092751 mem = 536881042 peak = 948981760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290654, 2, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (373, 682, 1753)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4513)
[M::mem_pestat] mean and std.dev: (783.45, 797.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5893)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.66, 118.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696860 reads in 75.652 CPU sec, 2.444 real sec
[M::process] read 696798 sequences (100000044 bp) in 2.314 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290699, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.00, 119.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 189, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (206.10, 88.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696798 reads in 76.301 CPU sec, 2.490 real sec
sorting block 44, #reads = 1092948 mem = 536877801 peak = 948981760
[M::process] read 695788 sequences (100000236 bp) in 3.033 CPU sec, 3.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290157, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (256.27, 122.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695788 reads in 76.153 CPU sec, 2.462 real sec
[M::process] read 698830 sequences (100000110 bp) in 2.283 CPU sec, 2.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290852, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.98, 118.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (523, 545, 1291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827)
[M::mem_pestat] mean and std.dev: (768.42, 555.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3595)
[M::mem_pestat] skip orientation RR
sorting block 45, #reads = 1092042 mem = 536883469 peak = 948981760
[M::mem_process_seqs] Processed 698830 reads in 79.537 CPU sec, 2.577 real sec
[M::process] read 707186 sequences (100000271 bp) in 3.315 CPU sec, 3.835 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293685, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.02, 108.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 707186 reads in 82.989 CPU sec, 2.681 real sec
[M::process] read 705020 sequences (100000100 bp) in 2.857 CPU sec, 3.207 real sec...
sorting block 46, #reads = 1092051 mem = 536880563 peak = 948981760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292893, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (234.46, 112.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705020 reads in 81.888 CPU sec, 2.650 real sec
[M::process] read 704024 sequences (100000196 bp) in 2.555 CPU sec, 2.787 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293234, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.59, 114.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1095690 mem = 536880032 peak = 948981760
[M::mem_process_seqs] Processed 704024 reads in 80.193 CPU sec, 2.595 real sec
[M::process] read 701962 sequences (100000021 bp) in 3.255 CPU sec, 3.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292307, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.35, 117.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701962 reads in 80.756 CPU sec, 2.612 real sec
[M::process] read 701098 sequences (100000181 bp) in 2.623 CPU sec, 2.877 real sec...
sorting block 48, #reads = 1095485 mem = 536884166 peak = 948981760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292193, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.06, 120.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701098 reads in 79.848 CPU sec, 2.588 real sec
[M::process] read 700926 sequences (100000205 bp) in 3.286 CPU sec, 3.753 real sec...
sorting block 49, #reads = 1094581 mem = 536879190 peak = 948981760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291711, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.15, 120.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700926 reads in 79.525 CPU sec, 2.574 real sec
[M::process] read 700774 sequences (100000165 bp) in 2.615 CPU sec, 2.969 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291999, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.12, 120.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700774 reads in 83.802 CPU sec, 2.725 real sec
[M::process] read 700736 sequences (100000026 bp) in 3.031 CPU sec, 3.369 real sec...
sorting block 50, #reads = 1092535 mem = 536879969 peak = 948981760
[M::process] read 700384 sequences (100000148 bp) in 1.829 CPU sec, 2.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292478, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 194, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (279.82, 150.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.56, 119.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699716 sequences (100000053 bp) in 1.439 CPU sec, 1.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291624, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (247.84, 120.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700736 reads in 146.554 CPU sec, 4.614 real sec
[M::mem_process_seqs] Processed 700384 reads in 94.146 CPU sec, 2.976 real sec
[M::process] read 698680 sequences (100000213 bp) in 1.847 CPU sec, 1.965 real sec...
sorting block 51, #reads = 1092866 mem = 536878404 peak = 948981760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291489, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.50, 119.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (280, 1186, 1717)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4591)
[M::mem_pestat] mean and std.dev: (969.33, 857.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6028)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290770, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.46, 121.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (290.50, 89.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RR
[M::process] read 698326 sequences (100000158 bp) in 2.908 CPU sec, 3.307 real sec...
[M::mem_process_seqs] Processed 699716 reads in 146.679 CPU sec, 4.719 real sec
[M::mem_process_seqs] Processed 698680 reads in 92.926 CPU sec, 2.958 real sec
sorting block 52, #reads = 1091966 mem = 536877888 peak = 948981760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291142, 14, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (360, 825, 1635)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4185)
[M::mem_pestat] mean and std.dev: (824.08, 538.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5460)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.35, 121.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 519, 752)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1876)
[M::mem_pestat] mean and std.dev: (480.85, 398.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2438)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 698172 sequences (100000090 bp) in 2.790 CPU sec, 3.141 real sec...
[M::mem_process_seqs] Processed 698326 reads in 82.590 CPU sec, 2.726 real sec
sorting block 53, #reads = 1091546 mem = 536881654 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291090, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.84, 121.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698172 reads in 82.923 CPU sec, 2.719 real sec
[M::process] read 697702 sequences (100000074 bp) in 3.052 CPU sec, 3.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290784, 9, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (252.04, 121.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 152, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 353)
[M::mem_pestat] mean and std.dev: (155.88, 23.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 423)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697702 reads in 81.535 CPU sec, 2.675 real sec
sorting block 54, #reads = 1091630 mem = 536879954 peak = 994742272
[M::process] read 697378 sequences (100000007 bp) in 3.355 CPU sec, 4.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290586, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.69, 121.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 401, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (323.33, 104.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697378 reads in 83.395 CPU sec, 2.708 real sec
sorting block 55, #reads = 1090417 mem = 536882722 peak = 994742272
[M::process] read 697068 sequences (100000109 bp) in 3.405 CPU sec, 3.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290204, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.39, 123.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 707262 sequences (100000287 bp) in 2.378 CPU sec, 2.588 real sec...
[M::mem_process_seqs] Processed 697068 reads in 82.384 CPU sec, 2.656 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293849, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (227.84, 108.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1089354 mem = 536878084 peak = 994742272
[M::mem_process_seqs] Processed 707262 reads in 90.917 CPU sec, 2.952 real sec
[M::process] read 706702 sequences (100000127 bp) in 3.143 CPU sec, 3.571 real sec...
[M::process] read 705190 sequences (100000127 bp) in 1.719 CPU sec, 1.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 293891, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (229.94, 109.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 747)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705136 sequences (100000185 bp) in 1.480 CPU sec, 1.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 293238, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 216, 1333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3605)
[M::mem_pestat] mean and std.dev: (559.42, 638.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4741)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 210, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (234.16, 111.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 703866 sequences (100000299 bp) in 1.460 CPU sec, 1.460 real sec...
[M::mem_process_seqs] Processed 706702 reads in 159.598 CPU sec, 5.027 real sec
sorting block 57, #reads = 1090916 mem = 536880802 peak = 994742272
[M::mem_process_seqs] Processed 705190 reads in 102.326 CPU sec, 3.250 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293694, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (234.63, 111.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 769)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (401, 401, 685)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253)
[M::mem_pestat] mean and std.dev: (465.30, 171.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1537)
[M::mem_pestat] skip orientation RR
sorting block 58, #reads = 1090386 mem = 536879349 peak = 994742272
[M::process] read 702720 sequences (100000285 bp) in 3.061 CPU sec, 3.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292978, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (237.02, 113.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705136 reads in 163.010 CPU sec, 5.204 real sec
[M::mem_process_seqs] Processed 703866 reads in 105.143 CPU sec, 3.336 real sec
[M::process] read 702448 sequences (100000226 bp) in 1.838 CPU sec, 1.884 real sec...
sorting block 59, #reads = 1090092 mem = 536883619 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292515, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.96, 115.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701130 sequences (100000268 bp) in 2.531 CPU sec, 2.810 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 292295, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.41, 115.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701890 sequences (100000062 bp) in 1.490 CPU sec, 1.493 real sec...
[M::mem_process_seqs] Processed 702720 reads in 159.965 CPU sec, 5.104 real sec
[M::mem_process_seqs] Processed 702448 reads in 104.449 CPU sec, 3.334 real sec
sorting block 60, #reads = 1089942 mem = 536879833 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292107, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.98, 117.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700676 sequences (100000104 bp) in 2.932 CPU sec, 3.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291906, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.85, 116.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701130 reads in 158.060 CPU sec, 5.046 real sec
[M::mem_process_seqs] Processed 701890 reads in 103.666 CPU sec, 3.294 real sec
[M::process] read 700826 sequences (100000078 bp) in 2.177 CPU sec, 2.344 real sec...
sorting block 61, #reads = 1089561 mem = 536880092 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291722, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (246.11, 119.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699374 sequences (100000286 bp) in 2.694 CPU sec, 3.009 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291979, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (243.59, 117.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700676 reads in 153.475 CPU sec, 4.925 real sec
sorting block 62, #reads = 1090736 mem = 536878726 peak = 994742272
[M::mem_process_seqs] Processed 700826 reads in 100.322 CPU sec, 3.204 real sec
[M::process] read 698854 sequences (100000237 bp) in 2.837 CPU sec, 3.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291296, 13, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (250.56, 122.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 190, 841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2143)
[M::mem_pestat] mean and std.dev: (421.92, 372.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2794)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 63, #reads = 1091314 mem = 536877760 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290834, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.67, 122.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699374 reads in 150.697 CPU sec, 4.870 real sec
[M::mem_process_seqs] Processed 698854 reads in 98.537 CPU sec, 3.177 real sec
[M::process] read 698744 sequences (100000060 bp) in 3.347 CPU sec, 3.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290632, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (251.24, 122.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 64, #reads = 1091255 mem = 536878741 peak = 994742272
[M::mem_process_seqs] Processed 698744 reads in 84.501 CPU sec, 2.823 real sec
[M::process] read 697614 sequences (100000074 bp) in 2.811 CPU sec, 3.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291173, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (254.22, 124.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 65, #reads = 1091116 mem = 536881389 peak = 994742272
[M::mem_process_seqs] Processed 697614 reads in 86.024 CPU sec, 2.841 real sec
[M::process] read 699368 sequences (100000061 bp) in 2.681 CPU sec, 2.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291144, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.16, 121.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699368 reads in 94.375 CPU sec, 3.079 real sec
[M::process] read 705950 sequences (100000065 bp) in 3.140 CPU sec, 3.584 real sec...
sorting block 66, #reads = 1088825 mem = 536879936 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293250, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (231.49, 110.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705950 reads in 86.301 CPU sec, 2.807 real sec
[M::process] read 703458 sequences (100000295 bp) in 3.407 CPU sec, 3.944 real sec...
sorting block 67, #reads = 1088133 mem = 536877512 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292645, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 360, 774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916)
[M::mem_pestat] mean and std.dev: (422.22, 314.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2487)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (237.07, 112.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 703458 reads in 84.202 CPU sec, 2.735 real sec
[M::process] read 702556 sequences (100000239 bp) in 3.250 CPU sec, 3.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292601, 10, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.57, 114.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (742, 1023, 1301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419)
[M::mem_pestat] mean and std.dev: (711.62, 424.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2978)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 219, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (89, 394)
[M::mem_pestat] mean and std.dev: (222.90, 66.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 490)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702556 reads in 81.770 CPU sec, 2.650 real sec
[M::process] read 701766 sequences (100000197 bp) in 2.588 CPU sec, 2.879 real sec...
sorting block 68, #reads = 1093315 mem = 536883023 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292342, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.90, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701766 reads in 80.800 CPU sec, 2.628 real sec
[M::process] read 701710 sequences (100000071 bp) in 3.388 CPU sec, 3.888 real sec...
sorting block 69, #reads = 1094091 mem = 536878247 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292550, 0, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 321, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277)
[M::mem_pestat] mean and std.dev: (294.30, 135.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1640)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.42, 117.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701710 reads in 79.045 CPU sec, 2.564 real sec
[M::process] read 701242 sequences (100000037 bp) in 2.342 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292270, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.09, 118.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701242 reads in 79.992 CPU sec, 2.601 real sec
[M::process] read 700752 sequences (100000019 bp) in 3.212 CPU sec, 3.680 real sec...
sorting block 70, #reads = 1095114 mem = 536879831 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291660, 8, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 400, 615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595)
[M::mem_pestat] mean and std.dev: (327.22, 221.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2085)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.07, 118.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700752 reads in 83.036 CPU sec, 2.707 real sec
[M::process] read 700726 sequences (100000088 bp) in 2.928 CPU sec, 3.159 real sec...
sorting block 71, #reads = 1094068 mem = 536882467 peak = 994742272
[M::process] read 699166 sequences (100000148 bp) in 1.975 CPU sec, 2.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292280, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 190, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (211.70, 60.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.61, 118.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 233, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (207.67, 54.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 585)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 699270 sequences (100000210 bp) in 1.470 CPU sec, 1.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291506, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 276, 663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1631)
[M::mem_pestat] mean and std.dev: (412.30, 310.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2115)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (248.89, 119.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700726 reads in 148.402 CPU sec, 4.677 real sec
[M::mem_process_seqs] Processed 699166 reads in 95.995 CPU sec, 3.033 real sec
[M::process] read 698500 sequences (100000183 bp) in 1.758 CPU sec, 1.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291680, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (277, 289, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (300.08, 66.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.63, 121.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 72, #reads = 1093145 mem = 536880101 peak = 994742272
[M::process] read 698320 sequences (100000026 bp) in 2.272 CPU sec, 2.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291211, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 322, 713)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1673)
[M::mem_pestat] mean and std.dev: (416.60, 288.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2153)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.47, 121.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699270 reads in 149.272 CPU sec, 4.769 real sec
[M::process] read 698258 sequences (100000154 bp) in 1.540 CPU sec, 1.541 real sec...
[M::mem_process_seqs] Processed 698500 reads in 97.654 CPU sec, 3.113 real sec
sorting block 73, #reads = 1093134 mem = 536876880 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290923, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.44, 121.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698426 sequences (100000289 bp) in 1.923 CPU sec, 2.008 real sec...
[M::process] read 698466 sequences (100000217 bp) in 1.519 CPU sec, 1.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290779, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.20, 120.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698320 reads in 148.707 CPU sec, 4.751 real sec
[M::mem_process_seqs] Processed 698258 reads in 96.084 CPU sec, 3.074 real sec
sorting block 74, #reads = 1091642 mem = 536881130 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291173, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.19, 121.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 510, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104)
[M::mem_pestat] mean and std.dev: (325.44, 170.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1401)
[M::mem_pestat] skip orientation RR
[M::process] read 698198 sequences (100000251 bp) in 3.146 CPU sec, 3.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290969, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 221, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (253.27, 155.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.78, 120.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698426 reads in 149.653 CPU sec, 4.791 real sec
[M::mem_process_seqs] Processed 698466 reads in 98.351 CPU sec, 3.137 real sec
sorting block 75, #reads = 1090759 mem = 536876890 peak = 994742272
[M::process] read 699218 sequences (100000161 bp) in 2.747 CPU sec, 3.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290808, 8, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.75, 120.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 738, 1139)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2907)
[M::mem_pestat] mean and std.dev: (739.46, 567.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3791)
[M::mem_pestat] skip orientation RR
sorting block 76, #reads = 1090397 mem = 536879350 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290839, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 464, 638)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528)
[M::mem_pestat] mean and std.dev: (484.73, 348.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1973)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.95, 119.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 709356 sequences (100000070 bp) in 2.631 CPU sec, 2.949 real sec...
[M::mem_process_seqs] Processed 698198 reads in 162.161 CPU sec, 5.213 real sec
[M::mem_process_seqs] Processed 699218 reads in 111.975 CPU sec, 3.568 real sec
[M::process] read 706488 sequences (100000271 bp) in 2.327 CPU sec, 2.463 real sec...
sorting block 77, #reads = 1088737 mem = 536884304 peak = 994742272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 294580, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 200, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568)
[M::mem_pestat] mean and std.dev: (222.85, 104.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704292 sequences (100000095 bp) in 2.752 CPU sec, 3.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293802, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.16, 109.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 709356 reads in 166.935 CPU sec, 5.329 real sec
[M::mem_process_seqs] Processed 706488 reads in 107.138 CPU sec, 3.410 real sec
sorting block 78, #reads = 1085054 mem = 536883207 peak = 994742272
[M::process] read 703610 sequences (100000201 bp) in 2.797 CPU sec, 3.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293035, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.62, 112.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703092 sequences (100000046 bp) in 2.211 CPU sec, 2.299 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292752, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.15, 114.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704292 reads in 164.118 CPU sec, 5.264 real sec
[M::mem_process_seqs] Processed 703610 reads in 109.253 CPU sec, 3.475 real sec
sorting block 79, #reads = 1092687 mem = 536878812 peak = 994861056
[M::process] read 701750 sequences (100000107 bp) in 2.399 CPU sec, 2.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292610, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.70, 115.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1089788 mem = 536876994 peak = 994861056
[M::process] read 701320 sequences (100000260 bp) in 2.292 CPU sec, 2.388 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292573, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (242.71, 117.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703092 reads in 157.491 CPU sec, 5.047 real sec
[M::mem_process_seqs] Processed 701750 reads in 102.372 CPU sec, 3.251 real sec
[M::process] read 699952 sequences (100000106 bp) in 2.246 CPU sec, 2.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292196, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.74, 116.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 81, #reads = 1090361 mem = 536878785 peak = 994861056
[M::process] read 698976 sequences (100000251 bp) in 2.653 CPU sec, 2.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291781, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.44, 118.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701320 reads in 157.401 CPU sec, 5.047 real sec
[M::mem_process_seqs] Processed 699952 reads in 102.698 CPU sec, 3.266 real sec
[M::process] read 699258 sequences (100000193 bp) in 2.676 CPU sec, 2.978 real sec...
sorting block 82, #reads = 1090548 mem = 536879604 peak = 994861056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290795, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.21, 119.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291078, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.65, 118.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698976 reads in 155.944 CPU sec, 5.029 real sec
[M::process] read 698684 sequences (100000271 bp) in 3.135 CPU sec, 3.568 real sec...
[M::mem_process_seqs] Processed 699258 reads in 102.720 CPU sec, 3.293 real sec
sorting block 83, #reads = 1090189 mem = 536880851 peak = 994861056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291033, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.48, 119.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698790 sequences (100000112 bp) in 2.293 CPU sec, 2.428 real sec...
[M::mem_process_seqs] Processed 698684 reads in 85.373 CPU sec, 2.836 real sec
sorting block 84, #reads = 1089979 mem = 536878300 peak = 994861056
[M::process] read 698638 sequences (100000085 bp) in 2.279 CPU sec, 2.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290849, 11, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.22, 119.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 210, 719)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1925)
[M::mem_pestat] mean and std.dev: (291.90, 286.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2528)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 335, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (293.90, 155.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698588 sequences (100000292 bp) in 1.858 CPU sec, 2.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290733, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (337, 1550, 2479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6763)
[M::mem_pestat] mean and std.dev: (1688.70, 1739.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8905)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.91, 120.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 196, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (212.86, 76.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 705)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698790 reads in 151.102 CPU sec, 4.815 real sec
[M::mem_process_seqs] Processed 698638 reads in 99.347 CPU sec, 3.156 real sec
[M::process] read 697564 sequences (100000181 bp) in 1.972 CPU sec, 2.042 real sec...
sorting block 85, #reads = 1090481 mem = 536878494 peak = 994861056
[M::process] read 697846 sequences (100000247 bp) in 2.070 CPU sec, 2.167 real sec...
[M::process] read 47812 sequences (6845884 bp) in 0.290 CPU sec, 0.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290409, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.94, 122.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697564 reads in 85.474 CPU sec, 2.738 real sec
sorting block 86, #reads = 1089840 mem = 536878378 peak = 994861056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291466, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.39, 120.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 182, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (242.62, 129.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290950, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.61, 121.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698588 reads in 233.964 CPU sec, 7.561 real sec
[M::mem_process_seqs] Processed 697846 reads in 114.101 CPU sec, 3.742 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 19946, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (247.11, 118.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 47812 reads in 6.690 CPU sec, 0.234 real sec
sorting block 87, #reads = 1090749 mem = 536878342 peak = 994861056
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96562592
overall: 69895081984 mem 12668.671 user 776.127 sys 742.710 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L007\tPU:L007\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 742.743 sec; CPU: 13444.802 sec
sorting block 88, #reads = 922202 mem = 454745541 peak = 1035440128
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 5260461596
output reads: 97052421
bam_mem_sort: 89 calls 35.462 user 0.814 sys 36.372 real
bam_write: 89 calls 91.112 user 2.713 sys 94.286 real
execute: 1 calls 21.763 user 1.836 sys 773.055 real
merge_files: 1 calls 21.739 user 1.814 sys 28.812 real
parse_chunk: 1422 calls 45.468 user 13.617 sys 60.524 real
read_chunk: 3840 calls 49.950 user 46.916 sys 435.046 real
sort_block: 1 calls 41.440 user 46.467 sys 421.864 real
write_chunk: 2416 calls 21.299 user 1.494 sys 27.779 real
overall: 2788884480 mem 1103.495 user 74.825 sys 773.076 real
ls: cannot access '*.cram': No such file or directory