.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1029_N5D-T1-TDNA-1_A23MHWWLT4_1;L005;0002)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/38/cf5c53472dbd51ee2f5b9b5cca9dab/.command.log
Size: 136.3 KB
Task: DAQ:SENTIEON_BWAMEM (1029_N5D-T1-TDNA-1_A23MHWWLT4_1;L005;0002)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/23/33d13c7d5c008e8f4e46c4506ed3c8/1029_N5D-T1-TDNA-1_A23MHWWLT4_1/0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/38/cf5c53472dbd51ee2f5b9b5cca9dab/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/23/33d13c7d5c008e8f4e46c4506ed3c8/1029_N5D-T1-TDNA-1_A23MHWWLT4_1/0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/38/cf5c53472dbd51ee2f5b9b5cca9dab/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1848.679 CPU sec, 88.973 real sec
[M::process] read 686810 sequences (100000233 bp) in 1.693 CPU sec, 1.695 real sec...
[M::process] read 685026 sequences (100000005 bp) in 1.919 CPU sec, 2.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279593, 5, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 356, 889)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267)
[M::mem_pestat] mean and std.dev: (431.64, 304.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2956)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 309, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (329.45, 162.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 275, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133)
[M::mem_pestat] mean and std.dev: (361.07, 233.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1452)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684526 sequences (100000198 bp) in 1.913 CPU sec, 2.205 real sec...
[M::process] read 683420 sequences (100000050 bp) in 1.966 CPU sec, 2.271 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 279034, 6, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 226, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (217.59, 88.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (339.70, 164.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 307, 1084)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2806)
[M::mem_pestat] mean and std.dev: (583.38, 717.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3667)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686810 reads in 224.699 CPU sec, 8.294 real sec
[M::mem_process_seqs] Processed 685026 reads in 151.014 CPU sec, 5.659 real sec
sorting block 0, #reads = 1071876 mem = 536879356 peak = 563982336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279026, 12, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 269, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (257.09, 102.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (344.90, 166.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 2180, 4651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13659)
[M::mem_pestat] mean and std.dev: (1855.42, 1802.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18163)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 491, 624)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1460)
[M::mem_pestat] mean and std.dev: (443.64, 320.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1878)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682722 sequences (100000175 bp) in 2.464 CPU sec, 2.925 real sec...
[M::process] read 682524 sequences (100000149 bp) in 2.065 CPU sec, 2.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278632, 7, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 221, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (262.24, 197.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (348.97, 166.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 232, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1585)
[M::mem_pestat] mean and std.dev: (473.86, 438.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2227)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684526 reads in 218.890 CPU sec, 7.381 real sec
[M::mem_process_seqs] Processed 683420 reads in 148.251 CPU sec, 5.123 real sec
sorting block 1, #reads = 1071834 mem = 536878518 peak = 691384320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278543, 6, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 240, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (253.55, 127.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (353.00, 167.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 297, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269)
[M::mem_pestat] mean and std.dev: (324.44, 170.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1630)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681890 sequences (100000278 bp) in 2.465 CPU sec, 3.099 real sec...
[M::process] read 681218 sequences (100000285 bp) in 2.055 CPU sec, 2.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277885, 12, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 263, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280)
[M::mem_pestat] mean and std.dev: (307.16, 174.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1649)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 342, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (355.30, 168.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1111, 3000, 6412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17014)
[M::mem_pestat] mean and std.dev: (3195.08, 2440.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22315)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (292, 341, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078)
[M::mem_pestat] mean and std.dev: (428.59, 217.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682722 reads in 224.048 CPU sec, 7.764 real sec
[M::mem_process_seqs] Processed 682524 reads in 154.869 CPU sec, 5.475 real sec
sorting block 2, #reads = 1070337 mem = 536880184 peak = 824401920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 277215, 5, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 256, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (292.05, 149.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (356.28, 166.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 207, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (235.74, 108.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681246 sequences (100000069 bp) in 2.995 CPU sec, 3.929 real sec...
[M::process] read 681346 sequences (100000265 bp) in 2.191 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277608, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 398, 714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1694)
[M::mem_pestat] mean and std.dev: (440.92, 334.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2184)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 344, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (355.93, 164.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 282, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (279.31, 118.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681890 reads in 241.350 CPU sec, 8.970 real sec
sorting block 3, #reads = 1069626 mem = 536876332 peak = 824401920
[M::mem_process_seqs] Processed 681218 reads in 162.856 CPU sec, 6.230 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277834, 15, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 296, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (299.62, 171.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (355.92, 165.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (277, 311, 1836)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4954)
[M::mem_pestat] mean and std.dev: (1050.07, 1222.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6513)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 335, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (341.71, 158.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 4, #reads = 1066944 mem = 536878737 peak = 837632000
[M::process] read 681204 sequences (100000194 bp) in 3.002 CPU sec, 3.540 real sec...
[M::process] read 681010 sequences (100000137 bp) in 1.930 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 277070, 4, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 237, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (287.31, 174.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (349.82, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 239, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (240.89, 105.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681246 reads in 250.234 CPU sec, 9.704 real sec
[M::mem_process_seqs] Processed 681346 reads in 163.336 CPU sec, 6.296 real sec
sorting block 5, #reads = 1067601 mem = 536878625 peak = 837632000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 277784, 5, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 335, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406)
[M::mem_pestat] mean and std.dev: (362.91, 236.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1806)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (356.67, 166.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 269, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (292.97, 139.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685878 sequences (100000079 bp) in 3.524 CPU sec, 3.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 278274, 5, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 296, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (297.48, 135.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 348, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (361.79, 170.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 378, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (331.28, 132.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681204 reads in 221.852 CPU sec, 8.011 real sec
[M::process] read 684482 sequences (100000209 bp) in 2.598 CPU sec, 2.988 real sec...
[M::mem_process_seqs] Processed 681010 reads in 153.839 CPU sec, 5.579 real sec
sorting block 6, #reads = 1067140 mem = 536877654 peak = 837632000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278709, 11, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 280, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (279.00, 161.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 316, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (336.17, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 229, 1419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3863)
[M::mem_pestat] mean and std.dev: (560.82, 582.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5085)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 252, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (245.17, 104.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684156 sequences (100000166 bp) in 2.954 CPU sec, 3.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278907, 4, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (262.00, 130.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (342.03, 164.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 234, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (291.75, 147.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683722 sequences (100000156 bp) in 2.369 CPU sec, 2.461 real sec...
[M::mem_process_seqs] Processed 685878 reads in 217.918 CPU sec, 7.936 real sec
[M::mem_process_seqs] Processed 684482 reads in 148.356 CPU sec, 5.676 real sec
sorting block 7, #reads = 1067331 mem = 536881314 peak = 837632000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278900, 7, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (118, 195, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (244.11, 169.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (345.90, 166.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 307, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079)
[M::mem_pestat] mean and std.dev: (297.42, 168.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1377)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683130 sequences (100000143 bp) in 3.460 CPU sec, 4.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278126, 4, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 229, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (229.96, 94.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (349.92, 167.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 351, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (340.53, 157.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684156 reads in 213.139 CPU sec, 7.666 real sec
sorting block 8, #reads = 1071918 mem = 536879336 peak = 837632000
[M::mem_process_seqs] Processed 683722 reads in 150.087 CPU sec, 5.530 real sec
[M::process] read 682294 sequences (100000062 bp) in 2.896 CPU sec, 3.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278261, 10, 59)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 210, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499)
[M::mem_pestat] mean and std.dev: (186.68, 64.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (350.72, 166.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 15, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1431)
[M::mem_pestat] mean and std.dev: (227.11, 390.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1903)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 208, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (262.75, 124.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 9, #reads = 1072142 mem = 536878872 peak = 887857152
[M::process] read 682412 sequences (100000089 bp) in 2.982 CPU sec, 3.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 277940, 8, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 339, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1308)
[M::mem_pestat] mean and std.dev: (342.65, 204.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1684)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (353.60, 167.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 381, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (383.52, 158.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1347)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683130 reads in 215.064 CPU sec, 7.871 real sec
[M::process] read 681722 sequences (100000100 bp) in 2.392 CPU sec, 2.645 real sec...
[M::mem_process_seqs] Processed 682294 reads in 150.608 CPU sec, 5.731 real sec
sorting block 10, #reads = 1070780 mem = 536880741 peak = 887857152
[M::process] read 681784 sequences (100000252 bp) in 3.606 CPU sec, 4.340 real sec...
[M::process] read 681876 sequences (100000038 bp) in 2.751 CPU sec, 2.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278142, 9, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 397, 892)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2198)
[M::mem_pestat] mean and std.dev: (488.36, 316.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2851)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 340, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (353.94, 166.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 306, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (351.24, 202.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681784 reads in 119.048 CPU sec, 4.378 real sec
sorting block 11, #reads = 1069835 mem = 536883018 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277168, 8, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 334, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243)
[M::mem_pestat] mean and std.dev: (349.86, 206.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1578)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (354.50, 166.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 278, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (310.20, 130.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681722 reads in 246.006 CPU sec, 10.012 real sec
[M::process] read 681252 sequences (100000167 bp) in 3.516 CPU sec, 3.807 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278955, 10, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 345, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (275.91, 110.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 341, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (354.92, 168.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (21, 973, 2378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7092)
[M::mem_pestat] mean and std.dev: (1209.20, 1176.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9449)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (306.61, 128.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278077, 9, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 206, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (249.88, 163.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (352.12, 166.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 262, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158)
[M::mem_pestat] mean and std.dev: (322.16, 261.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1495)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682412 reads in 457.692 CPU sec, 17.282 real sec
[M::mem_process_seqs] Processed 681876 reads in 178.341 CPU sec, 6.472 real sec
sorting block 12, #reads = 1068427 mem = 536881083 peak = 887857152
[M::process] read 681232 sequences (100000059 bp) in 2.963 CPU sec, 3.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 277493, 7, 61)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 225, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (232.62, 105.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 340, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (355.14, 166.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 275, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (329.65, 184.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1451)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 13, #reads = 1069378 mem = 536878634 peak = 887857152
[M::process] read 685708 sequences (100000075 bp) in 2.978 CPU sec, 3.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278482, 7, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 254, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (267.79, 146.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (361.66, 171.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 279, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (323.05, 145.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681252 reads in 207.931 CPU sec, 7.128 real sec
[M::mem_process_seqs] Processed 681232 reads in 146.986 CPU sec, 5.010 real sec
[M::process] read 684212 sequences (100000163 bp) in 3.037 CPU sec, 3.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279452, 5, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 248, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (239.18, 85.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 316, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (336.06, 165.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 268, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693)
[M::mem_pestat] mean and std.dev: (259.83, 77.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 14, #reads = 1069008 mem = 536884377 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 279266, 4, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 216, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (191.19, 60.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 684)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (343.64, 166.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 270, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (284.49, 158.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683410 sequences (100000036 bp) in 3.668 CPU sec, 4.343 real sec...
[M::mem_process_seqs] Processed 685708 reads in 206.142 CPU sec, 6.829 real sec
[M::process] read 682870 sequences (100000082 bp) in 3.305 CPU sec, 3.547 real sec...
sorting block 15, #reads = 1069415 mem = 536882310 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279051, 5, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 224, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (269.27, 135.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (350.90, 169.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 317, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (330.61, 133.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683410 reads in 119.822 CPU sec, 3.835 real sec
[M::process] read 682112 sequences (100000071 bp) in 2.882 CPU sec, 3.108 real sec...
[M::mem_process_seqs] Processed 684212 reads in 347.717 CPU sec, 11.253 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279039, 8, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 202, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (199.38, 69.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (356.90, 172.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 300, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (310.64, 161.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 16, #reads = 1071530 mem = 536878739 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278515, 8, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 169, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (253.09, 169.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (358.98, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 365, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1288)
[M::mem_pestat] mean and std.dev: (409.82, 262.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1655)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682870 reads in 226.319 CPU sec, 7.183 real sec
[M::process] read 682702 sequences (100000263 bp) in 3.489 CPU sec, 3.671 real sec...
[M::mem_process_seqs] Processed 682112 reads in 136.619 CPU sec, 4.339 real sec
sorting block 17, #reads = 1071703 mem = 536878504 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277870, 6, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 263, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322)
[M::mem_pestat] mean and std.dev: (304.19, 202.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1696)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 340, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (356.71, 172.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062)
[M::mem_pestat] mean and std.dev: (339.52, 155.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1341)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681584 sequences (100000161 bp) in 3.671 CPU sec, 3.910 real sec...
[M::mem_process_seqs] Processed 682702 reads in 109.134 CPU sec, 3.572 real sec
sorting block 18, #reads = 1070349 mem = 536880399 peak = 887857152
[M::process] read 681346 sequences (100000024 bp) in 2.842 CPU sec, 2.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277812, 6, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 235, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109)
[M::mem_pestat] mean and std.dev: (305.05, 200.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1420)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (359.33, 170.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 252, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (297.71, 151.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681612 sequences (100000047 bp) in 2.300 CPU sec, 2.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277627, 6, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 222, 611)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1485)
[M::mem_pestat] mean and std.dev: (250.55, 156.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1922)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (359.62, 170.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 366, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (292.89, 122.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681584 reads in 189.758 CPU sec, 5.969 real sec
[M::mem_process_seqs] Processed 681346 reads in 131.670 CPU sec, 4.127 real sec
[M::process] read 682076 sequences (100000111 bp) in 3.228 CPU sec, 3.362 real sec...
sorting block 19, #reads = 1069845 mem = 536879230 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277824, 7, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 278, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (337.45, 201.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (356.70, 170.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 271, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (270.21, 126.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681206 sequences (100000245 bp) in 2.896 CPU sec, 3.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 278216, 9, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 279, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (285.46, 113.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (356.69, 171.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 287, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (287.61, 110.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681612 reads in 183.718 CPU sec, 5.838 real sec
[M::mem_process_seqs] Processed 682076 reads in 125.418 CPU sec, 3.948 real sec
[M::process] read 680864 sequences (100000119 bp) in 2.220 CPU sec, 2.300 real sec...
sorting block 20, #reads = 1068908 mem = 536879367 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277826, 7, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 265, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (275.11, 152.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 344, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (358.95, 169.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (310, 394, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 763)
[M::mem_pestat] mean and std.dev: (355.23, 115.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686104 sequences (100000193 bp) in 3.052 CPU sec, 3.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277720, 9, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 289, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (326.00, 152.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (364.58, 173.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 250, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (312.11, 169.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683900 sequences (100000116 bp) in 1.890 CPU sec, 1.891 real sec...
[M::mem_process_seqs] Processed 681206 reads in 178.692 CPU sec, 5.651 real sec
sorting block 21, #reads = 1068567 mem = 536880049 peak = 887857152
[M::mem_process_seqs] Processed 680864 reads in 124.910 CPU sec, 3.946 real sec
sorting block 22, #reads = 1067071 mem = 536880009 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278814, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 286, 566)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270)
[M::mem_pestat] mean and std.dev: (398.81, 300.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1622)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 332, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (351.07, 172.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 387, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083)
[M::mem_pestat] mean and std.dev: (341.53, 191.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683082 sequences (100000199 bp) in 3.076 CPU sec, 3.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 279269, 8, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 337, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (313.02, 146.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 317, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (338.99, 169.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 257, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (257.30, 81.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683900 reads in 174.855 CPU sec, 5.506 real sec
[M::process] read 682132 sequences (100000099 bp) in 2.310 CPU sec, 2.308 real sec...
[M::mem_process_seqs] Processed 686104 reads in 199.218 CPU sec, 6.287 real sec
sorting block 23, #reads = 1068120 mem = 536879226 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278748, 6, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 274, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (266.66, 123.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 338, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (355.07, 172.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 383, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (350.16, 132.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681986 sequences (100000153 bp) in 3.447 CPU sec, 3.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278341, 3, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 278, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (281.03, 139.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 345, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (361.24, 174.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 300, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394)
[M::mem_pestat] mean and std.dev: (359.46, 172.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1788)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683082 reads in 164.108 CPU sec, 5.170 real sec
[M::process] read 682460 sequences (100000070 bp) in 2.285 CPU sec, 2.310 real sec...
[M::mem_process_seqs] Processed 682132 reads in 185.412 CPU sec, 5.851 real sec
sorting block 24, #reads = 1072564 mem = 536886249 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 278351, 6, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 210, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165)
[M::mem_pestat] mean and std.dev: (266.86, 149.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 348, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (362.67, 174.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 204, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (271.12, 172.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681266 sequences (100000109 bp) in 3.096 CPU sec, 3.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278393, 8, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 286, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (276.13, 125.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 343, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (358.94, 172.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 279, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (295.65, 128.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681986 reads in 164.419 CPU sec, 5.203 real sec
[M::process] read 681158 sequences (100000022 bp) in 2.155 CPU sec, 2.183 real sec...
[M::mem_process_seqs] Processed 682460 reads in 113.410 CPU sec, 3.565 real sec
sorting block 25, #reads = 1070669 mem = 536879956 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277844, 9, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189)
[M::mem_pestat] mean and std.dev: (346.10, 221.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1512)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 348, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (362.08, 171.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 286, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (288.88, 113.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681496 sequences (100000242 bp) in 3.441 CPU sec, 3.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 277980, 6, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 339, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (291.05, 121.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (356.18, 167.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 370, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (323.44, 131.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681266 reads in 164.952 CPU sec, 5.228 real sec
sorting block 26, #reads = 1070290 mem = 536879321 peak = 887857152
[M::mem_process_seqs] Processed 681158 reads in 113.440 CPU sec, 3.566 real sec
[M::process] read 681150 sequences (100000295 bp) in 2.633 CPU sec, 2.741 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278579, 7, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 218, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (248.43, 139.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (355.04, 167.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 289, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (291.71, 151.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 27, #reads = 1069066 mem = 536879102 peak = 887857152
[M::process] read 681030 sequences (100000094 bp) in 2.572 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277783, 5, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 185, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (221.47, 129.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (356.41, 168.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 315, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226)
[M::mem_pestat] mean and std.dev: (392.22, 265.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1552)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681496 reads in 164.525 CPU sec, 5.218 real sec
[M::process] read 680596 sequences (100000300 bp) in 2.039 CPU sec, 2.074 real sec...
[M::mem_process_seqs] Processed 681150 reads in 113.806 CPU sec, 3.579 real sec
sorting block 28, #reads = 1068728 mem = 536877967 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278021, 3, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 203, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (220.88, 136.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 768)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (358.70, 169.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 304, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (317.91, 136.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686714 sequences (100000197 bp) in 2.904 CPU sec, 3.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277792, 7, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 270, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (270.34, 127.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 350, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (364.15, 171.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (244.00, 83.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681030 reads in 161.977 CPU sec, 5.154 real sec
[M::mem_process_seqs] Processed 680596 reads in 111.543 CPU sec, 3.515 real sec
[M::process] read 687566 sequences (100000103 bp) in 2.441 CPU sec, 2.522 real sec...
sorting block 29, #reads = 1068167 mem = 536878768 peak = 887857152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279919, 3, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 283, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (304.57, 174.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 314, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (336.84, 168.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 351, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (340.23, 164.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685644 sequences (100000048 bp) in 2.796 CPU sec, 2.930 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280288, 4, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 302, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (275.26, 112.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 310, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (332.31, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 345, 969)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533)
[M::mem_pestat] mean and std.dev: (590.73, 547.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3315)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684694 sequences (100000169 bp) in 1.800 CPU sec, 1.800 real sec...
[M::mem_process_seqs] Processed 686714 reads in 176.041 CPU sec, 5.578 real sec
sorting block 30, #reads = 1067819 mem = 536880312 peak = 887857152
[M::mem_process_seqs] Processed 687566 reads in 121.002 CPU sec, 3.819 real sec
sorting block 31, #reads = 1067614 mem = 536880297 peak = 933220352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 279892, 8, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 268, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (275.64, 121.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 323, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (340.63, 166.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 247, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (278.22, 108.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684358 sequences (100000281 bp) in 3.189 CPU sec, 3.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279436, 11, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 302, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (255.14, 142.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1341)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 330, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (346.17, 167.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3208, 6458, 6458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12958)
[M::mem_pestat] mean and std.dev: (4426.55, 2678.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16208)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 242, 664)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1658)
[M::mem_pestat] mean and std.dev: (467.45, 437.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2219)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684320 sequences (100000268 bp) in 1.980 CPU sec, 1.975 real sec...
[M::mem_process_seqs] Processed 685644 reads in 169.357 CPU sec, 5.339 real sec
[M::mem_process_seqs] Processed 684694 reads in 189.694 CPU sec, 6.000 real sec
sorting block 32, #reads = 1069579 mem = 536877969 peak = 933220352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279517, 4, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 255, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (259.62, 121.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 921)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (346.58, 166.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 249, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (266.71, 143.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684170 sequences (100000007 bp) in 3.030 CPU sec, 3.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278962, 1, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 174, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490)
[M::mem_pestat] mean and std.dev: (185.42, 92.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 609)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (346.92, 166.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 300, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (312.67, 162.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683746 sequences (100000098 bp) in 1.910 CPU sec, 1.906 real sec...
[M::mem_process_seqs] Processed 684358 reads in 170.266 CPU sec, 5.369 real sec
[M::mem_process_seqs] Processed 684320 reads in 190.925 CPU sec, 6.041 real sec
sorting block 33, #reads = 1069198 mem = 536884920 peak = 933220352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278916, 3, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 210, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (229.22, 113.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 332, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (348.12, 167.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 312, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213)
[M::mem_pestat] mean and std.dev: (347.33, 220.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1554)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683256 sequences (100000096 bp) in 3.465 CPU sec, 3.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279137, 3, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 286, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361)
[M::mem_pestat] mean and std.dev: (323.65, 197.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1763)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 334, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (349.02, 167.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 258, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (241.17, 101.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684170 reads in 175.031 CPU sec, 5.522 real sec
[M::process] read 682272 sequences (100000206 bp) in 2.071 CPU sec, 2.083 real sec...
[M::mem_process_seqs] Processed 683746 reads in 118.107 CPU sec, 3.716 real sec
sorting block 34, #reads = 1068351 mem = 536882778 peak = 933220352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278409, 1, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 237, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124)
[M::mem_pestat] mean and std.dev: (284.14, 163.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1433)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (348.71, 164.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 270, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (273.51, 126.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681992 sequences (100000258 bp) in 2.870 CPU sec, 2.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278381, 7, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 230, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (237.59, 85.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (351.11, 164.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 318, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172)
[M::mem_pestat] mean and std.dev: (327.85, 170.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1497)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685678 sequences (100000006 bp) in 1.920 CPU sec, 1.921 real sec...
[M::mem_process_seqs] Processed 683256 reads in 178.012 CPU sec, 5.650 real sec
sorting block 35, #reads = 1067009 mem = 536877518 peak = 933220352
[M::mem_process_seqs] Processed 682272 reads in 120.721 CPU sec, 3.810 real sec
sorting block 36, #reads = 1065540 mem = 536879194 peak = 1024782336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278117, 6, 60)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (251, 300, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (328.16, 139.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (355.07, 167.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 374, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (323.79, 131.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 693358 sequences (100000223 bp) in 3.376 CPU sec, 3.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279612, 12, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 219, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (238.21, 110.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 872)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 320, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (339.34, 166.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (404, 498, 871)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1805)
[M::mem_pestat] mean and std.dev: (427.00, 283.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2272)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 262, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (261.22, 101.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 689806 sequences (100000055 bp) in 2.190 CPU sec, 2.194 real sec...
[M::mem_process_seqs] Processed 681992 reads in 177.951 CPU sec, 5.604 real sec
[M::mem_process_seqs] Processed 685678 reads in 201.895 CPU sec, 6.377 real sec
sorting block 37, #reads = 1063775 mem = 536884595 peak = 1024782336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282585, 2, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 197, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (234.27, 112.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 279, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (302.69, 151.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 233, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (265.93, 134.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687682 sequences (100000260 bp) in 3.420 CPU sec, 3.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281838, 2, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 266, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1235)
[M::mem_pestat] mean and std.dev: (311.63, 169.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1590)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 297, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (317.35, 155.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 400, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200)
[M::mem_pestat] mean and std.dev: (349.77, 178.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1528)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685968 sequences (100000112 bp) in 2.520 CPU sec, 2.527 real sec...
[M::mem_process_seqs] Processed 693358 reads in 191.957 CPU sec, 6.045 real sec
sorting block 38, #reads = 1061324 mem = 536880605 peak = 1024782336
[M::mem_process_seqs] Processed 689806 reads in 215.790 CPU sec, 6.819 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281179, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 227, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (259.71, 160.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 308, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (326.70, 158.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 306, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105)
[M::mem_pestat] mean and std.dev: (343.76, 163.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1399)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685728 sequences (100000061 bp) in 3.179 CPU sec, 3.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 280211, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 310, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (287.71, 105.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (334.44, 161.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 327, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243)
[M::mem_pestat] mean and std.dev: (338.06, 182.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1585)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687682 reads in 189.135 CPU sec, 5.953 real sec
[M::process] read 684756 sequences (100000262 bp) in 2.710 CPU sec, 2.707 real sec...
sorting block 39, #reads = 1063740 mem = 536878402 peak = 1024782336
[M::mem_process_seqs] Processed 685968 reads in 134.639 CPU sec, 4.230 real sec
sorting block 40, #reads = 1064138 mem = 536883528 peak = 1024782336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 279660, 3, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 254, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (235.79, 102.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 888)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 327, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (344.32, 167.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 292, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (299.82, 138.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683716 sequences (100000130 bp) in 3.239 CPU sec, 3.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 280090, 4, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 291, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (271.14, 133.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 321, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (337.02, 162.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 265, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (295.94, 170.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682780 sequences (100000062 bp) in 2.250 CPU sec, 2.244 real sec...
[M::mem_process_seqs] Processed 684756 reads in 190.751 CPU sec, 6.026 real sec
[M::mem_process_seqs] Processed 685728 reads in 214.501 CPU sec, 6.796 real sec
sorting block 41, #reads = 1065757 mem = 536883455 peak = 1024782336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279055, 7, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 265, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286)
[M::mem_pestat] mean and std.dev: (350.73, 207.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1658)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 336, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (353.31, 171.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 397, 642)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1460)
[M::mem_pestat] mean and std.dev: (376.59, 259.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1869)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682216 sequences (100000183 bp) in 3.800 CPU sec, 3.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279119, 3, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 217, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (225.36, 111.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 338, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (354.77, 170.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 273, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (281.21, 131.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683716 reads in 189.933 CPU sec, 5.979 real sec
[M::process] read 683072 sequences (100000266 bp) in 2.711 CPU sec, 2.722 real sec...
[M::mem_process_seqs] Processed 682780 reads in 212.513 CPU sec, 6.699 real sec
sorting block 42, #reads = 1065965 mem = 536882002 peak = 1024782336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278549, 10, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 247, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (257.54, 121.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 345, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (361.33, 175.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (289, 1158, 6085)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17677)
[M::mem_pestat] mean and std.dev: (2495.00, 2543.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 23473)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 359, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (320.61, 134.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 687666 sequences (100000019 bp) in 2.927 CPU sec, 2.981 real sec...
[M::process] read 685618 sequences (100000234 bp) in 1.950 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279050, 7, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 336, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (346.31, 215.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 339, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (358.37, 176.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 312, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (283.28, 129.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682216 reads in 186.341 CPU sec, 5.888 real sec
sorting block 43, #reads = 1064606 mem = 536879096 peak = 1024782336
[M::mem_process_seqs] Processed 683072 reads in 131.427 CPU sec, 4.157 real sec
sorting block 44, #reads = 1060352 mem = 536878197 peak = 1024782336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 280001, 2, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 262, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (245.20, 110.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 322, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (340.74, 167.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 249, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (237.82, 88.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684540 sequences (100000049 bp) in 3.050 CPU sec, 3.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280509, 12, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 284, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281)
[M::mem_pestat] mean and std.dev: (360.91, 286.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1639)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 308, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (329.80, 164.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 1806, 1831)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4981)
[M::mem_pestat] mean and std.dev: (866.80, 784.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6556)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 253, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (282.57, 112.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683554 sequences (100000046 bp) in 1.950 CPU sec, 1.946 real sec...
[M::mem_process_seqs] Processed 685618 reads in 177.677 CPU sec, 5.606 real sec
[M::mem_process_seqs] Processed 687666 reads in 201.069 CPU sec, 6.367 real sec
sorting block 45, #reads = 1068603 mem = 536878388 peak = 1024782336
[M::process] read 683784 sequences (100000218 bp) in 2.263 CPU sec, 2.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279434, 10, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 277, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (248.86, 107.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 326, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (343.87, 166.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (56, 409, 648)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832)
[M::mem_pestat] mean and std.dev: (307.89, 262.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2424)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 365, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1333)
[M::mem_pestat] mean and std.dev: (384.38, 222.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1716)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683838 sequences (100000173 bp) in 1.790 CPU sec, 1.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 279254, 4, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 232, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (267.33, 152.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 333, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (349.71, 168.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 268, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (282.38, 140.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279307, 4, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 248, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (219.10, 86.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 683)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (348.31, 168.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (256.68, 101.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684540 reads in 249.448 CPU sec, 7.940 real sec
[M::mem_process_seqs] Processed 683554 reads in 192.889 CPU sec, 6.173 real sec
sorting block 46, #reads = 1069430 mem = 536878437 peak = 1024782336
[M::mem_process_seqs] Processed 683784 reads in 213.971 CPU sec, 6.851 real sec
[M::process] read 682996 sequences (100000026 bp) in 2.471 CPU sec, 2.560 real sec...
sorting block 47, #reads = 1069702 mem = 536880688 peak = 1024782336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279630, 2, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 214, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (265.39, 138.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 330, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (348.22, 169.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 284, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (240.90, 96.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682498 sequences (100000044 bp) in 2.313 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 278534, 7, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 301, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (330.45, 145.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 337, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (354.12, 171.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 280, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (267.87, 99.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683838 reads in 185.462 CPU sec, 5.870 real sec
[M::process] read 682654 sequences (100000192 bp) in 2.131 CPU sec, 2.163 real sec...
[M::mem_process_seqs] Processed 682996 reads in 134.319 CPU sec, 4.237 real sec
sorting block 48, #reads = 1067806 mem = 536878565 peak = 1024782336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278523, 10, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 220, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (221.15, 97.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (356.95, 172.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (74, 273, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (184.00, 95.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 252, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (282.59, 109.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682308 sequences (100000210 bp) in 2.571 CPU sec, 2.661 real sec...
[M::process] read 682212 sequences (100000017 bp) in 2.020 CPU sec, 2.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278541, 3, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (271.84, 156.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 338, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (356.62, 173.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 278, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138)
[M::mem_pestat] mean and std.dev: (286.50, 159.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1453)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682498 reads in 179.490 CPU sec, 5.675 real sec
sorting block 49, #reads = 1066660 mem = 536885452 peak = 1024782336
[M::mem_process_seqs] Processed 682654 reads in 119.642 CPU sec, 4.000 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278511, 4, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 310, 751)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907)
[M::mem_pestat] mean and std.dev: (478.12, 410.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2485)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (357.02, 171.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 299, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (318.21, 120.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689750 sequences (100000045 bp) in 2.819 CPU sec, 2.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278804, 9, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (267, 422, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (362.87, 135.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 341, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (358.90, 174.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 374, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (358.58, 154.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689916 sequences (100000011 bp) in 2.680 CPU sec, 2.677 real sec...
[M::mem_process_seqs] Processed 682308 reads in 195.396 CPU sec, 6.359 real sec
sorting block 50, #reads = 1066036 mem = 536879634 peak = 1024782336
[M::mem_process_seqs] Processed 682212 reads in 221.037 CPU sec, 6.981 real sec
sorting block 51, #reads = 1063679 mem = 536877289 peak = 1024782336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280853, 5, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 226, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165)
[M::mem_pestat] mean and std.dev: (279.67, 176.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1495)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 297, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (322.54, 165.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 309, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (298.22, 108.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686940 sequences (100000162 bp) in 3.451 CPU sec, 3.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281640, 13, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 238, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (246.63, 84.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 299, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (320.25, 159.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (28, 42, 1105)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3259)
[M::mem_pestat] mean and std.dev: (296.58, 446.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4336)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 285, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (289.54, 122.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685186 sequences (100000131 bp) in 2.360 CPU sec, 2.362 real sec...
[M::mem_process_seqs] Processed 689750 reads in 214.713 CPU sec, 6.772 real sec
[M::process] read 684548 sequences (100000264 bp) in 2.389 CPU sec, 2.400 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 280097, 3, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 267, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (302.04, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (341.49, 164.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 224, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (270.44, 134.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685186 reads in 113.320 CPU sec, 3.593 real sec
sorting block 52, #reads = 1057457 mem = 536880459 peak = 1024782336
[M::mem_process_seqs] Processed 689916 reads in 378.543 CPU sec, 11.993 real sec
sorting block 53, #reads = 1063059 mem = 536879427 peak = 1024782336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 280755, 4, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 257, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131)
[M::mem_pestat] mean and std.dev: (295.63, 190.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1442)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 315, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (332.92, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 237, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (257.91, 105.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 679)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683404 sequences (100000247 bp) in 2.365 CPU sec, 2.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279642, 9, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 252, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (283.23, 128.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 328, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (344.71, 166.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 503, 948)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2306)
[M::mem_pestat] mean and std.dev: (561.39, 498.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2985)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682554 sequences (100000215 bp) in 2.300 CPU sec, 2.300 real sec...
[M::mem_process_seqs] Processed 686940 reads in 307.970 CPU sec, 9.739 real sec
[M::mem_process_seqs] Processed 684548 reads in 136.937 CPU sec, 4.331 real sec
sorting block 54, #reads = 1063060 mem = 536881537 peak = 1024782336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278599, 8, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 325, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256)
[M::mem_pestat] mean and std.dev: (321.17, 173.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1625)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 336, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (352.47, 168.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 268, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (281.39, 126.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682426 sequences (100000063 bp) in 3.025 CPU sec, 3.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278924, 8, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 308, 576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436)
[M::mem_pestat] mean and std.dev: (357.14, 339.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1866)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 336, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (351.62, 168.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 325, 553)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1251)
[M::mem_pestat] mean and std.dev: (355.96, 215.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1600)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681840 sequences (100000082 bp) in 2.350 CPU sec, 2.348 real sec...
[M::mem_process_seqs] Processed 682554 reads in 189.371 CPU sec, 5.974 real sec
sorting block 55, #reads = 1063710 mem = 536884075 peak = 1024782336
[M::mem_process_seqs] Processed 683404 reads in 219.849 CPU sec, 6.965 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 278295, 10, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 214, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1743)
[M::mem_pestat] mean and std.dev: (406.48, 370.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2277)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 336, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (353.13, 169.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1093, 2089, 5065)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13009)
[M::mem_pestat] mean and std.dev: (3114.70, 2893.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16981)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 250, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.38, 104.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681518 sequences (100000032 bp) in 3.319 CPU sec, 3.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 278104, 7, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 219, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (250.77, 126.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 341, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (357.00, 170.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 309, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (300.37, 119.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682426 reads in 182.858 CPU sec, 5.760 real sec
[M::process] read 681294 sequences (100000239 bp) in 2.550 CPU sec, 2.552 real sec...
sorting block 56, #reads = 1063651 mem = 536880731 peak = 1024782336
[M::mem_process_seqs] Processed 681840 reads in 212.501 CPU sec, 6.731 real sec
[M::process] read 34814 sequences (5084450 bp) in 0.264 CPU sec, 0.269 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 278187, 7, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (264.21, 149.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 344, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (360.71, 172.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 266, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 505)
[M::mem_pestat] mean and std.dev: (248.90, 80.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 14088, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 327, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (346.40, 169.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278400, 10, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 237, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (262.35, 128.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 347, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (364.16, 175.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1254, 3499, 4960)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12372)
[M::mem_pestat] mean and std.dev: (2756.00, 1753.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16078)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 284, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (274.92, 120.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681518 reads in 173.701 CPU sec, 5.615 real sec
[M::mem_process_seqs] Processed 34814 reads in 98.510 CPU sec, 3.175 real sec
sorting block 57, #reads = 1064676 mem = 536878826 peak = 1024782336
[M::mem_process_seqs] Processed 681294 reads in 120.292 CPU sec, 3.967 real sec
sorting block 58, #reads = 1066624 mem = 536882425 peak = 1106743296
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 62922294
overall: 70022782976 mem 11517.146 user 337.430 sys 431.050 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 431.093 sec; CPU: 11854.579 sec
sorting block 59, #reads = 304633 mem = 153564705 peak = 1106743296
uniq header count 1
merging 60 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3997227080
output reads: 63292722
bam_mem_sort: 60 calls 36.313 user 0.665 sys 37.948 real
bam_write: 60 calls 28.252 user 1.680 sys 45.078 real
execute: 1 calls 22.201 user 1.404 sys 461.888 real
merge_files: 1 calls 22.176 user 1.370 sys 30.558 real
parse_chunk: 949 calls 53.683 user 13.277 sys 74.549 real
read_chunk: 2564 calls 28.805 user 17.952 sys 344.852 real
sort_block: 1 calls 19.382 user 17.499 sys 332.317 real
write_chunk: 1613 calls 21.672 user 1.080 sys 29.512 real
overall: 2877755392 mem 1160.983 user 45.554 sys 461.910 real
ls: cannot access '*.cram': No such file or directory