.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_bR4-T1-TDNA-1_A23MHWWLT4_1;L006;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/21/449ce36b5c4dd04861509accc3ec71/.command.err
Size: 234.9 KB
Task: DAQ:SENTIEON_BWAMEM (659_bR4-T1-TDNA-1_A23MHWWLT4_1;L006;0001)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_bR4-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1936.287 CPU sec, 109.035 real sec
[M::process] read 680036 sequences (100000271 bp) in 1.661 CPU sec, 1.665 real sec...
[M::process] read 677896 sequences (100000262 bp) in 2.093 CPU sec, 4.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282828, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 313, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (332.19, 153.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677432 sequences (100000214 bp) in 2.293 CPU sec, 4.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281546, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (346.33, 157.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680036 reads in 200.836 CPU sec, 11.855 real sec
[M::mem_process_seqs] Processed 677896 reads in 130.655 CPU sec, 6.975 real sec
[M::process] read 677194 sequences (100000169 bp) in 2.272 CPU sec, 3.064 real sec...
sorting block 0, #reads = 1081649 mem = 536881623 peak = 584028160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281685, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (349.93, 158.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677192 sequences (100000101 bp) in 2.661 CPU sec, 3.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280748, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (350.05, 157.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676712 sequences (100000219 bp) in 2.454 CPU sec, 2.686 real sec...
[M::mem_process_seqs] Processed 677432 reads in 196.525 CPU sec, 7.029 real sec
[M::mem_process_seqs] Processed 677194 reads in 129.749 CPU sec, 4.927 real sec
sorting block 1, #reads = 1079415 mem = 536877820 peak = 711430144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281775, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (352.00, 158.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676608 sequences (100000205 bp) in 2.728 CPU sec, 3.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281386, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 337, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (353.10, 157.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677192 reads in 196.362 CPU sec, 7.143 real sec
[M::process] read 676628 sequences (100000247 bp) in 2.346 CPU sec, 2.485 real sec...
[M::mem_process_seqs] Processed 676712 reads in 132.363 CPU sec, 4.896 real sec
sorting block 2, #reads = 1079669 mem = 536877794 peak = 815304704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281873, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 340, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (354.78, 158.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676234 sequences (100000235 bp) in 3.296 CPU sec, 3.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281485, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 341, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (355.63, 158.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676608 reads in 208.159 CPU sec, 8.014 real sec
[M::process] read 676032 sequences (100000035 bp) in 2.832 CPU sec, 3.007 real sec...
sorting block 3, #reads = 1079827 mem = 536880684 peak = 815304704
[M::mem_process_seqs] Processed 676628 reads in 130.210 CPU sec, 5.012 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281635, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (357.75, 159.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675922 sequences (100000092 bp) in 2.649 CPU sec, 3.410 real sec...
sorting block 4, #reads = 1079786 mem = 536879028 peak = 868343808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281791, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (360.01, 159.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676234 reads in 186.732 CPU sec, 6.555 real sec
[M::mem_process_seqs] Processed 676032 reads in 124.438 CPU sec, 4.457 real sec
[M::process] read 675766 sequences (100000236 bp) in 2.424 CPU sec, 2.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281680, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (362.31, 161.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1079104 mem = 536883202 peak = 868343808
[M::process] read 675590 sequences (100000152 bp) in 2.970 CPU sec, 3.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281662, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (363.95, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675922 reads in 184.702 CPU sec, 6.368 real sec
[M::mem_process_seqs] Processed 675766 reads in 124.383 CPU sec, 4.354 real sec
[M::process] read 675436 sequences (100000244 bp) in 2.493 CPU sec, 2.723 real sec...
sorting block 6, #reads = 1079754 mem = 536877148 peak = 868343808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281960, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 344, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (360.01, 159.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675448 sequences (100000127 bp) in 2.576 CPU sec, 3.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281391, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (365.89, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675590 reads in 183.450 CPU sec, 6.291 real sec
[M::mem_process_seqs] Processed 675436 reads in 124.382 CPU sec, 4.333 real sec
[M::process] read 675112 sequences (100000222 bp) in 2.724 CPU sec, 3.088 real sec...
sorting block 7, #reads = 1080280 mem = 536878525 peak = 868343808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281130, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (365.15, 162.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675034 sequences (100000224 bp) in 2.927 CPU sec, 3.663 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281431, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (366.50, 161.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675448 reads in 184.068 CPU sec, 6.358 real sec
[M::mem_process_seqs] Processed 675112 reads in 124.144 CPU sec, 4.320 real sec
sorting block 8, #reads = 1079883 mem = 536879543 peak = 868343808
[M::process] read 674990 sequences (100000099 bp) in 2.861 CPU sec, 3.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281712, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (368.42, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281321, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (367.88, 162.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674876 sequences (100000145 bp) in 2.749 CPU sec, 3.043 real sec...
sorting block 9, #reads = 1079223 mem = 536881922 peak = 880599040
[M::mem_process_seqs] Processed 675034 reads in 187.521 CPU sec, 6.290 real sec
[M::mem_process_seqs] Processed 674990 reads in 125.411 CPU sec, 4.187 real sec
[M::process] read 675170 sequences (100000167 bp) in 2.891 CPU sec, 3.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281178, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (366.82, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 10, #reads = 1079602 mem = 536881369 peak = 880599040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281661, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 353, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (367.16, 162.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674976 sequences (100000018 bp) in 3.052 CPU sec, 3.393 real sec...
[M::mem_process_seqs] Processed 674876 reads in 186.408 CPU sec, 6.216 real sec
[M::mem_process_seqs] Processed 675170 reads in 124.474 CPU sec, 4.179 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281213, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (366.59, 161.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674656 sequences (100000245 bp) in 2.968 CPU sec, 3.358 real sec...
[M::mem_process_seqs] Processed 674976 reads in 104.347 CPU sec, 3.565 real sec
sorting block 11, #reads = 1080343 mem = 536882085 peak = 880599040
[M::process] read 674680 sequences (100000065 bp) in 2.898 CPU sec, 3.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281252, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (367.27, 161.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674656 reads in 104.042 CPU sec, 3.513 real sec
sorting block 12, #reads = 1079671 mem = 536882920 peak = 880599040
[M::process] read 674656 sequences (100000247 bp) in 3.102 CPU sec, 3.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282006, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (363.64, 158.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281030, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (362.14, 158.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674596 sequences (100000096 bp) in 2.652 CPU sec, 3.048 real sec...
[M::mem_process_seqs] Processed 674680 reads in 181.719 CPU sec, 5.928 real sec
[M::mem_process_seqs] Processed 674656 reads in 122.428 CPU sec, 3.976 real sec
sorting block 13, #reads = 1078440 mem = 536879599 peak = 880599040
[M::process] read 674924 sequences (100000160 bp) in 2.897 CPU sec, 3.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281422, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (361.02, 157.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1078702 mem = 536878438 peak = 893988864
[M::process] read 674764 sequences (100000106 bp) in 2.558 CPU sec, 2.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281154, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 344, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (358.90, 156.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674596 reads in 182.017 CPU sec, 5.897 real sec
[M::mem_process_seqs] Processed 674924 reads in 122.362 CPU sec, 3.932 real sec
[M::process] read 674998 sequences (100000295 bp) in 2.724 CPU sec, 2.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280675, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 342, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (357.25, 155.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1079208 mem = 536879808 peak = 893988864
[M::process] read 675176 sequences (100000002 bp) in 2.620 CPU sec, 2.818 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281002, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 343, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (357.43, 155.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674764 reads in 181.629 CPU sec, 5.853 real sec
[M::mem_process_seqs] Processed 674998 reads in 120.859 CPU sec, 3.846 real sec
[M::process] read 674984 sequences (100000277 bp) in 2.642 CPU sec, 2.763 real sec...
sorting block 16, #reads = 1078664 mem = 536878505 peak = 893988864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281255, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 340, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (354.44, 153.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675084 sequences (100000213 bp) in 2.521 CPU sec, 2.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280954, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 342, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (356.30, 154.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675176 reads in 176.234 CPU sec, 5.628 real sec
[M::mem_process_seqs] Processed 674984 reads in 117.453 CPU sec, 3.706 real sec
[M::process] read 675042 sequences (100000091 bp) in 2.332 CPU sec, 2.401 real sec...
sorting block 17, #reads = 1078498 mem = 536877209 peak = 904724480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281123, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 341, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (356.41, 154.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675024 sequences (100000071 bp) in 2.620 CPU sec, 2.743 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280755, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 342, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (356.88, 155.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675084 reads in 167.280 CPU sec, 5.309 real sec
[M::process] read 674700 sequences (100000075 bp) in 2.098 CPU sec, 2.121 real sec...
sorting block 18, #reads = 1078287 mem = 536879254 peak = 904724480
[M::mem_process_seqs] Processed 675042 reads in 112.001 CPU sec, 3.530 real sec
sorting block 19, #reads = 1077887 mem = 536878141 peak = 904724480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280706, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 342, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (357.73, 155.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674778 sequences (100000249 bp) in 2.566 CPU sec, 2.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281133, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 345, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (358.95, 155.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675024 reads in 163.007 CPU sec, 5.164 real sec
[M::process] read 674560 sequences (100000253 bp) in 2.284 CPU sec, 2.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280798, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 345, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (359.37, 155.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674166 sequences (100000073 bp) in 2.381 CPU sec, 2.384 real sec...
[M::mem_process_seqs] Processed 674778 reads in 90.802 CPU sec, 2.857 real sec
sorting block 20, #reads = 1078126 mem = 536878926 peak = 904724480
[M::mem_process_seqs] Processed 674700 reads in 224.190 CPU sec, 7.071 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281259, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (363.42, 157.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1077051 mem = 536878286 peak = 904724480
[M::process] read 674340 sequences (100000077 bp) in 2.452 CPU sec, 2.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280728, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (366.79, 158.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674560 reads in 169.008 CPU sec, 5.339 real sec
[M::mem_process_seqs] Processed 674166 reads in 99.804 CPU sec, 3.135 real sec
[M::process] read 676732 sequences (100000055 bp) in 1.898 CPU sec, 1.923 real sec...
sorting block 22, #reads = 1077764 mem = 536878766 peak = 904724480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281300, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 351, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (364.84, 157.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677600 sequences (100000051 bp) in 1.982 CPU sec, 2.072 real sec...
[M::process] read 677384 sequences (100000066 bp) in 1.560 CPU sec, 1.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281880, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (351.78, 158.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674340 reads in 149.735 CPU sec, 4.748 real sec
[M::mem_process_seqs] Processed 676732 reads in 98.421 CPU sec, 3.111 real sec
sorting block 23, #reads = 1076228 mem = 536880056 peak = 904724480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282076, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (350.44, 159.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677170 sequences (100000161 bp) in 2.091 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281662, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (350.43, 159.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677000 sequences (100000277 bp) in 1.570 CPU sec, 1.566 real sec...
[M::mem_process_seqs] Processed 677600 reads in 133.249 CPU sec, 4.211 real sec
[M::mem_process_seqs] Processed 677384 reads in 85.210 CPU sec, 2.684 real sec
sorting block 24, #reads = 1080885 mem = 536876727 peak = 904724480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281255, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (351.88, 158.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676808 sequences (100000110 bp) in 2.119 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281552, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (353.47, 158.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677170 reads in 129.280 CPU sec, 4.082 real sec
[M::process] read 676856 sequences (100000264 bp) in 1.620 CPU sec, 1.620 real sec...
sorting block 25, #reads = 1082155 mem = 536879604 peak = 904724480
[M::mem_process_seqs] Processed 677000 reads in 83.038 CPU sec, 2.615 real sec
sorting block 26, #reads = 1082022 mem = 536886385 peak = 945766400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281759, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (354.45, 159.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676412 sequences (100000263 bp) in 2.000 CPU sec, 2.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281828, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 341, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (355.67, 159.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676808 reads in 126.067 CPU sec, 3.989 real sec
[M::process] read 676340 sequences (100000031 bp) in 1.658 CPU sec, 1.661 real sec...
[M::mem_process_seqs] Processed 676856 reads in 82.079 CPU sec, 2.578 real sec
sorting block 27, #reads = 1082470 mem = 536878479 peak = 945766400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281858, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (358.19, 159.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676066 sequences (100000091 bp) in 2.128 CPU sec, 2.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282060, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (359.19, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676412 reads in 126.743 CPU sec, 4.010 real sec
[M::process] read 676056 sequences (100000047 bp) in 1.653 CPU sec, 1.665 real sec...
[M::mem_process_seqs] Processed 676340 reads in 81.488 CPU sec, 2.560 real sec
sorting block 28, #reads = 1081038 mem = 536881239 peak = 945766400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281769, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (360.33, 159.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675878 sequences (100000187 bp) in 1.955 CPU sec, 2.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281376, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (361.69, 160.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676066 reads in 124.557 CPU sec, 3.942 real sec
[M::process] read 675698 sequences (100000016 bp) in 1.578 CPU sec, 1.580 real sec...
[M::mem_process_seqs] Processed 676056 reads in 81.065 CPU sec, 2.547 real sec
sorting block 29, #reads = 1081284 mem = 536880031 peak = 965046272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281096, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (362.23, 160.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675394 sequences (100000228 bp) in 1.966 CPU sec, 2.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281763, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (364.13, 161.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675878 reads in 123.531 CPU sec, 3.911 real sec
[M::process] read 675356 sequences (100000089 bp) in 1.623 CPU sec, 1.628 real sec...
sorting block 30, #reads = 1080815 mem = 536881187 peak = 965046272
[M::mem_process_seqs] Processed 675698 reads in 80.842 CPU sec, 2.542 real sec
sorting block 31, #reads = 1081080 mem = 536880191 peak = 972333056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281385, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (365.05, 161.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675254 sequences (100000197 bp) in 1.912 CPU sec, 1.980 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281268, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (362.62, 158.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675394 reads in 121.937 CPU sec, 3.860 real sec
[M::process] read 674934 sequences (100000004 bp) in 1.625 CPU sec, 1.621 real sec...
[M::mem_process_seqs] Processed 675356 reads in 79.498 CPU sec, 2.495 real sec
sorting block 32, #reads = 1080238 mem = 536879023 peak = 972333056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281553, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (365.36, 160.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675068 sequences (100000074 bp) in 2.005 CPU sec, 2.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280877, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (368.97, 162.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675254 reads in 122.030 CPU sec, 3.861 real sec
[M::mem_process_seqs] Processed 674934 reads in 80.128 CPU sec, 2.510 real sec
[M::process] read 675074 sequences (100000203 bp) in 1.797 CPU sec, 1.814 real sec...
sorting block 33, #reads = 1080139 mem = 536879668 peak = 972333056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281078, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 354, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (367.38, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675020 sequences (100000281 bp) in 1.916 CPU sec, 1.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280483, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 356, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (369.60, 162.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675068 reads in 123.868 CPU sec, 3.920 real sec
[M::process] read 674694 sequences (100000150 bp) in 1.616 CPU sec, 1.620 real sec...
[M::mem_process_seqs] Processed 675074 reads in 81.217 CPU sec, 2.552 real sec
sorting block 34, #reads = 1079500 mem = 536878542 peak = 999661568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280840, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (367.24, 160.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674420 sequences (100000262 bp) in 1.989 CPU sec, 2.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281612, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (368.39, 160.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675020 reads in 122.978 CPU sec, 3.888 real sec
[M::mem_process_seqs] Processed 674694 reads in 80.005 CPU sec, 2.510 real sec
sorting block 35, #reads = 1078351 mem = 536884732 peak = 999661568
[M::process] read 674724 sequences (100000056 bp) in 2.083 CPU sec, 2.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281254, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 358, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (372.35, 163.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1080459 mem = 536879048 peak = 1025232896
[M::process] read 674904 sequences (100000102 bp) in 2.453 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280782, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (366.02, 159.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674420 reads in 125.985 CPU sec, 3.999 real sec
[M::mem_process_seqs] Processed 674724 reads in 82.273 CPU sec, 2.583 real sec
[M::process] read 674656 sequences (100000076 bp) in 1.977 CPU sec, 2.037 real sec...
sorting block 37, #reads = 1079015 mem = 536879765 peak = 1025232896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281475, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 341, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (355.65, 153.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674774 sequences (100000298 bp) in 2.047 CPU sec, 2.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281380, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (362.57, 157.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674904 reads in 126.269 CPU sec, 3.998 real sec
[M::mem_process_seqs] Processed 674656 reads in 81.483 CPU sec, 2.558 real sec
[M::process] read 674508 sequences (100000132 bp) in 1.742 CPU sec, 1.777 real sec...
sorting block 38, #reads = 1078445 mem = 536881199 peak = 1025232896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281126, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (360.03, 156.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674894 sequences (100000040 bp) in 1.901 CPU sec, 1.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281330, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (359.73, 155.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674774 reads in 124.063 CPU sec, 3.930 real sec
[M::process] read 674752 sequences (100000238 bp) in 1.591 CPU sec, 1.601 real sec...
[M::mem_process_seqs] Processed 674508 reads in 80.455 CPU sec, 2.523 real sec
sorting block 39, #reads = 1079345 mem = 536883206 peak = 1025232896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281277, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 344, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (358.93, 156.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674850 sequences (100000062 bp) in 1.963 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281161, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 343, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (358.27, 155.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674894 reads in 122.390 CPU sec, 3.872 real sec
[M::process] read 674734 sequences (100000073 bp) in 1.567 CPU sec, 1.574 real sec...
sorting block 40, #reads = 1079024 mem = 536876271 peak = 1025232896
[M::mem_process_seqs] Processed 674752 reads in 79.166 CPU sec, 2.490 real sec
sorting block 41, #reads = 1078105 mem = 536878346 peak = 1029496832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280882, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 344, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (359.16, 156.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674780 sequences (100000062 bp) in 2.021 CPU sec, 2.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280795, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (360.42, 156.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674850 reads in 123.072 CPU sec, 3.895 real sec
[M::process] read 674824 sequences (100000032 bp) in 1.623 CPU sec, 1.633 real sec...
[M::mem_process_seqs] Processed 674734 reads in 79.999 CPU sec, 2.514 real sec
sorting block 42, #reads = 1078104 mem = 536878889 peak = 1029496832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281354, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (360.73, 157.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674808 sequences (100000133 bp) in 1.895 CPU sec, 1.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281917, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 344, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (358.37, 155.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674780 reads in 121.178 CPU sec, 3.837 real sec
[M::process] read 675080 sequences (100000113 bp) in 1.654 CPU sec, 1.662 real sec...
[M::mem_process_seqs] Processed 674824 reads in 79.847 CPU sec, 2.504 real sec
sorting block 43, #reads = 1079099 mem = 536877370 peak = 1029496832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281175, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 344, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (359.35, 156.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674748 sequences (100000111 bp) in 1.970 CPU sec, 2.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280903, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 344, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (359.11, 157.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674808 reads in 120.675 CPU sec, 3.818 real sec
[M::process] read 674486 sequences (100000181 bp) in 1.625 CPU sec, 1.631 real sec...
[M::mem_process_seqs] Processed 675080 reads in 79.259 CPU sec, 2.488 real sec
sorting block 44, #reads = 1079077 mem = 536877661 peak = 1029496832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281171, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (361.96, 157.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674560 sequences (100000123 bp) in 2.007 CPU sec, 2.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281577, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (365.29, 159.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674748 reads in 119.829 CPU sec, 3.791 real sec
[M::process] read 674420 sequences (100000087 bp) in 1.598 CPU sec, 1.609 real sec...
sorting block 45, #reads = 1077467 mem = 536881079 peak = 1050427392
[M::mem_process_seqs] Processed 674486 reads in 77.723 CPU sec, 2.442 real sec
sorting block 46, #reads = 1077724 mem = 536877176 peak = 1080463360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281372, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (366.36, 160.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677650 sequences (100000100 bp) in 2.058 CPU sec, 2.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281419, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (368.60, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674560 reads in 122.878 CPU sec, 3.893 real sec
[M::mem_process_seqs] Processed 674420 reads in 80.981 CPU sec, 2.547 real sec
[M::process] read 678268 sequences (100000245 bp) in 1.708 CPU sec, 1.716 real sec...
sorting block 47, #reads = 1076439 mem = 536879492 peak = 1080463360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281675, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (347.01, 157.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678368 sequences (100000157 bp) in 2.028 CPU sec, 2.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282067, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (346.04, 157.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677650 reads in 121.379 CPU sec, 3.845 real sec
[M::mem_process_seqs] Processed 678268 reads in 78.706 CPU sec, 2.467 real sec
[M::process] read 678102 sequences (100000226 bp) in 1.855 CPU sec, 1.870 real sec...
sorting block 48, #reads = 1077774 mem = 536880591 peak = 1080463360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282431, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (346.33, 157.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677774 sequences (100000057 bp) in 1.920 CPU sec, 1.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281510, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (345.84, 156.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678368 reads in 120.230 CPU sec, 3.810 real sec
[M::process] read 677818 sequences (100000040 bp) in 1.600 CPU sec, 1.608 real sec...
[M::mem_process_seqs] Processed 678102 reads in 78.364 CPU sec, 2.459 real sec
sorting block 49, #reads = 1081087 mem = 536879917 peak = 1080463360
[M::process] read 677478 sequences (100000106 bp) in 1.854 CPU sec, 1.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282233, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (348.83, 157.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677818 reads in 65.599 CPU sec, 2.054 real sec
[M::process] read 677262 sequences (100000227 bp) in 1.527 CPU sec, 1.527 real sec...
sorting block 50, #reads = 1081440 mem = 536879000 peak = 1080463360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282077, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (347.12, 157.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281506, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (349.86, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677774 reads in 183.475 CPU sec, 5.799 real sec
[M::process] read 676864 sequences (100000270 bp) in 1.793 CPU sec, 1.806 real sec...
[M::mem_process_seqs] Processed 677478 reads in 90.146 CPU sec, 2.841 real sec
sorting block 51, #reads = 1080878 mem = 536879408 peak = 1080463360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281946, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.31, 158.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676790 sequences (100000259 bp) in 2.387 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281507, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 340, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (354.23, 158.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677262 reads in 119.777 CPU sec, 3.796 real sec
[M::mem_process_seqs] Processed 676864 reads in 78.076 CPU sec, 2.453 real sec
sorting block 52, #reads = 1080132 mem = 536879736 peak = 1080463360
[M::process] read 676374 sequences (100000024 bp) in 1.976 CPU sec, 2.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282014, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (356.50, 159.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1079995 mem = 536880878 peak = 1080463360
[M::process] read 676740 sequences (100000038 bp) in 1.856 CPU sec, 1.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281456, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 344, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (357.43, 158.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676790 reads in 119.467 CPU sec, 3.782 real sec
[M::mem_process_seqs] Processed 676374 reads in 77.967 CPU sec, 2.444 real sec
[M::process] read 676124 sequences (100000191 bp) in 1.561 CPU sec, 1.574 real sec...
sorting block 54, #reads = 1079907 mem = 536880607 peak = 1080463360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281792, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 340, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (354.69, 159.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676186 sequences (100000013 bp) in 1.854 CPU sec, 1.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281990, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (361.70, 160.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676740 reads in 118.041 CPU sec, 3.742 real sec
[M::process] read 675594 sequences (100000153 bp) in 1.466 CPU sec, 1.470 real sec...
[M::mem_process_seqs] Processed 676124 reads in 76.215 CPU sec, 2.399 real sec
sorting block 55, #reads = 1080197 mem = 536882364 peak = 1080463360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281551, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (361.23, 161.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675688 sequences (100000069 bp) in 2.525 CPU sec, 2.616 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281320, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (362.73, 160.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676186 reads in 118.413 CPU sec, 3.748 real sec
[M::mem_process_seqs] Processed 675594 reads in 76.536 CPU sec, 2.406 real sec
sorting block 56, #reads = 1080277 mem = 536882419 peak = 1080463360
[M::process] read 675440 sequences (100000167 bp) in 2.705 CPU sec, 2.844 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281997, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (365.83, 162.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281234, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (367.82, 163.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675688 reads in 120.197 CPU sec, 3.840 real sec
[M::process] read 675596 sequences (100000068 bp) in 2.239 CPU sec, 2.334 real sec...
[M::mem_process_seqs] Processed 675440 reads in 79.473 CPU sec, 2.515 real sec
sorting block 57, #reads = 1079404 mem = 536878560 peak = 1080463360
[M::process] read 675308 sequences (100000044 bp) in 2.050 CPU sec, 2.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280913, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (365.92, 162.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1080214 mem = 536876724 peak = 1092833280
[M::process] read 675194 sequences (100000027 bp) in 1.597 CPU sec, 1.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281091, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (366.98, 162.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675596 reads in 121.090 CPU sec, 3.838 real sec
[M::mem_process_seqs] Processed 675308 reads in 77.960 CPU sec, 2.453 real sec
[M::process] read 675132 sequences (100000237 bp) in 1.886 CPU sec, 1.947 real sec...
sorting block 59, #reads = 1078905 mem = 536877523 peak = 1092833280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281676, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (367.32, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675186 sequences (100000179 bp) in 1.837 CPU sec, 1.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281064, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 353, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (367.01, 161.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675194 reads in 120.450 CPU sec, 3.813 real sec
[M::mem_process_seqs] Processed 675132 reads in 77.756 CPU sec, 2.439 real sec
[M::process] read 676010 sequences (100000059 bp) in 1.672 CPU sec, 1.698 real sec...
sorting block 60, #reads = 1079755 mem = 536881545 peak = 1092833280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281260, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (364.64, 160.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675680 sequences (100000123 bp) in 2.005 CPU sec, 2.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281866, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 335, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (350.91, 155.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675186 reads in 120.923 CPU sec, 3.821 real sec
[M::process] read 675744 sequences (100000097 bp) in 1.417 CPU sec, 1.420 real sec...
[M::mem_process_seqs] Processed 676010 reads in 78.372 CPU sec, 2.462 real sec
sorting block 61, #reads = 1079715 mem = 536878625 peak = 1092833280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280649, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 341, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (356.42, 157.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675190 sequences (100000025 bp) in 2.009 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281594, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 340, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (356.00, 157.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675680 reads in 120.729 CPU sec, 3.819 real sec
[M::process] read 674650 sequences (100000074 bp) in 1.458 CPU sec, 1.460 real sec...
sorting block 62, #reads = 1078015 mem = 536878416 peak = 1104375808
[M::mem_process_seqs] Processed 675744 reads in 77.887 CPU sec, 2.442 real sec
sorting block 63, #reads = 1078903 mem = 536878652 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281248, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (363.30, 160.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674770 sequences (100000152 bp) in 2.108 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281218, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (366.05, 160.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675190 reads in 119.701 CPU sec, 3.781 real sec
[M::process] read 674916 sequences (100000177 bp) in 1.625 CPU sec, 1.630 real sec...
[M::mem_process_seqs] Processed 674650 reads in 77.341 CPU sec, 2.424 real sec
sorting block 64, #reads = 1079017 mem = 536879538 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280986, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (364.71, 159.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674538 sequences (100000073 bp) in 1.934 CPU sec, 1.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281683, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (363.74, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674770 reads in 120.098 CPU sec, 3.804 real sec
[M::process] read 674678 sequences (100000027 bp) in 1.467 CPU sec, 1.473 real sec...
[M::mem_process_seqs] Processed 674916 reads in 77.552 CPU sec, 2.437 real sec
sorting block 65, #reads = 1078940 mem = 536878602 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281655, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (366.46, 161.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674830 sequences (100000032 bp) in 2.026 CPU sec, 2.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281192, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (364.79, 159.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674896 sequences (100000289 bp) in 1.460 CPU sec, 1.464 real sec...
[M::mem_process_seqs] Processed 674538 reads in 119.776 CPU sec, 3.781 real sec
sorting block 66, #reads = 1079603 mem = 536883079 peak = 1145901056
[M::mem_process_seqs] Processed 674678 reads in 76.788 CPU sec, 2.415 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280576, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (364.80, 160.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674604 sequences (100000194 bp) in 1.880 CPU sec, 1.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281503, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (364.11, 159.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674982 sequences (100000091 bp) in 1.490 CPU sec, 1.495 real sec...
[M::mem_process_seqs] Processed 674830 reads in 122.339 CPU sec, 3.868 real sec
sorting block 67, #reads = 1077488 mem = 536879779 peak = 1145901056
[M::mem_process_seqs] Processed 674896 reads in 79.089 CPU sec, 2.492 real sec
sorting block 68, #reads = 1078154 mem = 536880544 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280915, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (364.21, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674948 sequences (100000045 bp) in 2.098 CPU sec, 2.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280897, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (363.53, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673968 sequences (100000239 bp) in 1.480 CPU sec, 1.475 real sec...
[M::mem_process_seqs] Processed 674604 reads in 122.261 CPU sec, 3.858 real sec
[M::mem_process_seqs] Processed 674982 reads in 79.427 CPU sec, 2.498 real sec
sorting block 69, #reads = 1077141 mem = 536879410 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281142, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (365.11, 162.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673950 sequences (100000099 bp) in 1.936 CPU sec, 1.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281305, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 359, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (374.71, 165.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674948 reads in 117.767 CPU sec, 3.720 real sec
[M::process] read 677974 sequences (100000124 bp) in 1.466 CPU sec, 1.470 real sec...
[M::mem_process_seqs] Processed 673968 reads in 76.589 CPU sec, 2.409 real sec
sorting block 70, #reads = 1077271 mem = 536876554 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281021, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 358, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (373.20, 163.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678208 sequences (100000070 bp) in 2.050 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282671, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (346.46, 158.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677938 sequences (100000277 bp) in 1.440 CPU sec, 1.439 real sec...
[M::mem_process_seqs] Processed 673950 reads in 120.675 CPU sec, 3.812 real sec
sorting block 71, #reads = 1077529 mem = 536878440 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282026, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (346.63, 158.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677796 sequences (100000071 bp) in 1.549 CPU sec, 1.551 real sec...
[M::mem_process_seqs] Processed 678208 reads in 65.610 CPU sec, 2.071 real sec
[M::mem_process_seqs] Processed 677974 reads in 158.466 CPU sec, 5.007 real sec
sorting block 72, #reads = 1080248 mem = 536879118 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281500, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (346.80, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677512 sequences (100000242 bp) in 2.085 CPU sec, 2.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281730, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (347.92, 158.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677938 reads in 128.896 CPU sec, 4.079 real sec
[M::mem_process_seqs] Processed 677796 reads in 75.889 CPU sec, 2.383 real sec
[M::process] read 677472 sequences (100000128 bp) in 1.590 CPU sec, 1.614 real sec...
sorting block 73, #reads = 1082727 mem = 536879804 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281971, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (347.22, 156.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677556 sequences (100000112 bp) in 1.982 CPU sec, 2.041 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281115, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (348.76, 157.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677512 reads in 116.539 CPU sec, 3.686 real sec
[M::process] read 677604 sequences (100000125 bp) in 1.446 CPU sec, 1.451 real sec...
sorting block 74, #reads = 1082365 mem = 536880092 peak = 1145901056
[M::mem_process_seqs] Processed 677472 reads in 74.545 CPU sec, 2.344 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281242, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (350.07, 158.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 75, #reads = 1081602 mem = 536877728 peak = 1145901056
[M::process] read 677156 sequences (100000071 bp) in 2.098 CPU sec, 2.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281566, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (349.94, 159.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677556 reads in 119.277 CPU sec, 3.768 real sec
[M::process] read 676954 sequences (100000247 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_process_seqs] Processed 677604 reads in 77.016 CPU sec, 2.425 real sec
sorting block 76, #reads = 1081524 mem = 536881478 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282040, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (353.80, 160.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676882 sequences (100000116 bp) in 2.023 CPU sec, 2.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281750, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (354.95, 159.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676766 sequences (100000245 bp) in 1.420 CPU sec, 1.418 real sec...
[M::mem_process_seqs] Processed 677156 reads in 115.867 CPU sec, 3.657 real sec
[M::mem_process_seqs] Processed 676954 reads in 74.914 CPU sec, 2.359 real sec
sorting block 77, #reads = 1081248 mem = 536879693 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281647, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (355.58, 159.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676608 sequences (100000084 bp) in 2.022 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281283, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (353.88, 158.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676420 sequences (100000134 bp) in 1.430 CPU sec, 1.429 real sec...
[M::mem_process_seqs] Processed 676882 reads in 117.076 CPU sec, 3.695 real sec
[M::mem_process_seqs] Processed 676766 reads in 75.547 CPU sec, 2.378 real sec
sorting block 78, #reads = 1081165 mem = 536879725 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281377, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 343, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (357.88, 160.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676026 sequences (100000053 bp) in 2.047 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280990, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (358.82, 160.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675800 sequences (100000200 bp) in 1.420 CPU sec, 1.427 real sec...
[M::mem_process_seqs] Processed 676608 reads in 117.849 CPU sec, 3.720 real sec
sorting block 79, #reads = 1080586 mem = 536880621 peak = 1145901056
[M::mem_process_seqs] Processed 676420 reads in 75.676 CPU sec, 2.386 real sec
sorting block 80, #reads = 1081194 mem = 536878757 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281453, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (361.79, 162.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675926 sequences (100000040 bp) in 2.110 CPU sec, 2.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281385, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (361.62, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676026 reads in 117.946 CPU sec, 3.721 real sec
[M::process] read 675670 sequences (100000035 bp) in 1.520 CPU sec, 1.518 real sec...
[M::mem_process_seqs] Processed 675800 reads in 76.607 CPU sec, 2.408 real sec
sorting block 81, #reads = 1080540 mem = 536876914 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281714, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (364.71, 162.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675776 sequences (100000206 bp) in 2.032 CPU sec, 2.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281767, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (365.52, 163.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675458 sequences (100000140 bp) in 1.440 CPU sec, 1.434 real sec...
[M::mem_process_seqs] Processed 675926 reads in 118.310 CPU sec, 3.737 real sec
[M::mem_process_seqs] Processed 675670 reads in 76.166 CPU sec, 2.395 real sec
sorting block 82, #reads = 1080693 mem = 536878863 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281502, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (365.20, 163.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675252 sequences (100000209 bp) in 2.246 CPU sec, 2.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281446, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (367.69, 163.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675776 reads in 118.475 CPU sec, 3.740 real sec
[M::process] read 675148 sequences (100000189 bp) in 1.594 CPU sec, 1.600 real sec...
[M::mem_process_seqs] Processed 675458 reads in 76.419 CPU sec, 2.401 real sec
sorting block 83, #reads = 1079604 mem = 536880094 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280660, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (367.86, 162.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675220 sequences (100000269 bp) in 2.001 CPU sec, 2.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281105, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (365.60, 161.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675296 sequences (100000287 bp) in 1.460 CPU sec, 1.456 real sec...
[M::mem_process_seqs] Processed 675252 reads in 119.015 CPU sec, 3.760 real sec
sorting block 84, #reads = 1079919 mem = 536879568 peak = 1145901056
[M::mem_process_seqs] Processed 675148 reads in 75.892 CPU sec, 2.391 real sec
sorting block 85, #reads = 1079263 mem = 536878113 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281156, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (366.05, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675136 sequences (100000001 bp) in 2.131 CPU sec, 2.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281237, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (363.48, 159.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675220 reads in 120.468 CPU sec, 3.805 real sec
[M::process] read 674758 sequences (100000017 bp) in 1.490 CPU sec, 1.488 real sec...
[M::mem_process_seqs] Processed 675296 reads in 77.768 CPU sec, 2.443 real sec
sorting block 86, #reads = 1078964 mem = 536886372 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281425, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (365.08, 159.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674740 sequences (100000231 bp) in 2.092 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280395, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (365.53, 158.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674584 sequences (100000194 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_process_seqs] Processed 675136 reads in 120.101 CPU sec, 3.791 real sec
[M::mem_process_seqs] Processed 674758 reads in 77.813 CPU sec, 2.454 real sec
sorting block 87, #reads = 1079555 mem = 536886306 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280470, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (367.89, 160.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674472 sequences (100000233 bp) in 2.030 CPU sec, 2.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280797, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (367.55, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674740 reads in 119.877 CPU sec, 3.784 real sec
[M::process] read 674354 sequences (100000084 bp) in 1.490 CPU sec, 1.482 real sec...
sorting block 88, #reads = 1078268 mem = 536877418 peak = 1145901056
[M::mem_process_seqs] Processed 674584 reads in 77.179 CPU sec, 2.429 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281198, 2, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 353, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (366.77, 158.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (495, 1070, 3047)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8151)
[M::mem_pestat] mean and std.dev: (1455.81, 1097.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10703)
[M::mem_pestat] skip orientation RR
[M::process] read 674306 sequences (100000082 bp) in 1.905 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280816, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 351, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (364.91, 156.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674288 sequences (100000072 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_process_seqs] Processed 674472 reads in 119.868 CPU sec, 3.788 real sec
sorting block 89, #reads = 1077989 mem = 536877182 peak = 1145901056
[M::mem_process_seqs] Processed 674354 reads in 77.069 CPU sec, 2.432 real sec
sorting block 90, #reads = 1078046 mem = 536878768 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280865, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 353, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (366.52, 157.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674228 sequences (100000208 bp) in 2.081 CPU sec, 2.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281251, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (366.97, 158.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674358 sequences (100000177 bp) in 1.460 CPU sec, 1.454 real sec...
[M::mem_process_seqs] Processed 674306 reads in 120.115 CPU sec, 3.791 real sec
[M::mem_process_seqs] Processed 674288 reads in 77.858 CPU sec, 2.455 real sec
sorting block 91, #reads = 1078381 mem = 536882360 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281059, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (366.08, 157.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674334 sequences (100000131 bp) in 2.118 CPU sec, 2.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281045, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (364.44, 157.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674592 sequences (100000067 bp) in 1.440 CPU sec, 1.444 real sec...
[M::mem_process_seqs] Processed 674228 reads in 119.236 CPU sec, 3.762 real sec
[M::mem_process_seqs] Processed 674358 reads in 77.445 CPU sec, 2.439 real sec
sorting block 92, #reads = 1078020 mem = 536880312 peak = 1145901056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280606, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (362.46, 156.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674596 sequences (100000232 bp) in 2.051 CPU sec, 2.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281227, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (361.83, 156.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674094 sequences (100000156 bp) in 1.440 CPU sec, 1.436 real sec...
[M::mem_process_seqs] Processed 674334 reads in 118.957 CPU sec, 3.754 real sec
sorting block 93, #reads = 1077144 mem = 536878603 peak = 1145901056
[M::mem_process_seqs] Processed 674592 reads in 76.866 CPU sec, 2.423 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281208, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 349, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (362.86, 156.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676968 sequences (100000223 bp) in 2.126 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281157, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 352, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (366.21, 157.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674596 reads in 119.225 CPU sec, 3.760 real sec
[M::process] read 681692 sequences (100000189 bp) in 1.550 CPU sec, 1.559 real sec...
sorting block 94, #reads = 1076148 mem = 536878948 peak = 1145901056
[M::mem_process_seqs] Processed 674094 reads in 77.213 CPU sec, 2.429 real sec
sorting block 95, #reads = 1076654 mem = 536877059 peak = 1197039616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282086, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (352.83, 159.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679838 sequences (100000096 bp) in 2.120 CPU sec, 2.154 real sec...
[M::process] read 680088 sequences (100000159 bp) in 1.500 CPU sec, 1.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283856, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 303, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (325.78, 153.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676968 reads in 133.731 CPU sec, 4.229 real sec
[M::mem_process_seqs] Processed 681692 reads in 87.646 CPU sec, 2.772 real sec
sorting block 96, #reads = 1074856 mem = 536880196 peak = 1197039616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283127, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (338.85, 158.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679638 sequences (100000278 bp) in 2.125 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283216, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (339.32, 159.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679486 sequences (100000110 bp) in 1.519 CPU sec, 1.516 real sec...
[M::mem_process_seqs] Processed 679838 reads in 129.840 CPU sec, 4.099 real sec
[M::mem_process_seqs] Processed 680088 reads in 83.630 CPU sec, 2.645 real sec
sorting block 97, #reads = 1078610 mem = 536880356 peak = 1197039616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282953, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (338.47, 157.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678134 sequences (100000136 bp) in 1.992 CPU sec, 2.032 real sec...
[M::process] read 677910 sequences (100000060 bp) in 1.510 CPU sec, 1.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283111, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 322, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (340.70, 158.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679638 reads in 131.799 CPU sec, 4.168 real sec
sorting block 98, #reads = 1077675 mem = 536882523 peak = 1198993408
[M::mem_process_seqs] Processed 679486 reads in 84.889 CPU sec, 2.680 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282601, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (348.18, 159.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677714 sequences (100000221 bp) in 2.192 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282962, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (350.81, 160.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677470 sequences (100000032 bp) in 1.530 CPU sec, 1.526 real sec...
[M::mem_process_seqs] Processed 678134 reads in 126.564 CPU sec, 3.994 real sec
sorting block 99, #reads = 1076906 mem = 536885211 peak = 1198993408
[M::mem_process_seqs] Processed 677910 reads in 80.383 CPU sec, 2.534 real sec
sorting block 100, #reads = 1078601 mem = 536880152 peak = 1202147328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282152, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.68, 160.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677068 sequences (100000153 bp) in 2.159 CPU sec, 2.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282375, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (354.80, 161.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676908 sequences (100000083 bp) in 1.500 CPU sec, 1.502 real sec...
[M::mem_process_seqs] Processed 677714 reads in 127.578 CPU sec, 4.029 real sec
[M::mem_process_seqs] Processed 677470 reads in 81.579 CPU sec, 2.571 real sec
sorting block 101, #reads = 1078415 mem = 536877744 peak = 1202147328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282129, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (358.64, 162.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676528 sequences (100000115 bp) in 2.115 CPU sec, 2.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282544, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 344, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (359.50, 162.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676962 sequences (100000091 bp) in 1.500 CPU sec, 1.507 real sec...
[M::mem_process_seqs] Processed 677068 reads in 123.894 CPU sec, 3.911 real sec
[M::mem_process_seqs] Processed 676908 reads in 79.904 CPU sec, 2.515 real sec
sorting block 102, #reads = 1077169 mem = 536879172 peak = 1202147328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282066, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 347, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (361.70, 162.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676456 sequences (100000063 bp) in 2.168 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282149, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (356.16, 159.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675750 sequences (100000186 bp) in 1.510 CPU sec, 1.511 real sec...
[M::mem_process_seqs] Processed 676528 reads in 126.404 CPU sec, 3.990 real sec
sorting block 103, #reads = 1077675 mem = 536879135 peak = 1202147328
[M::mem_process_seqs] Processed 676962 reads in 82.277 CPU sec, 2.595 real sec
sorting block 104, #reads = 1077047 mem = 536881168 peak = 1202147328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282258, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 349, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (364.39, 164.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676102 sequences (100000268 bp) in 2.171 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281011, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 355, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (368.92, 164.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676300 sequences (100000009 bp) in 1.530 CPU sec, 1.530 real sec...
[M::mem_process_seqs] Processed 676456 reads in 129.796 CPU sec, 4.097 real sec
[M::mem_process_seqs] Processed 675750 reads in 83.664 CPU sec, 2.642 real sec
sorting block 105, #reads = 1077145 mem = 536878058 peak = 1202147328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281984, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 351, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (365.76, 164.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675820 sequences (100000040 bp) in 2.159 CPU sec, 2.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281869, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 351, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (366.51, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675600 sequences (100000126 bp) in 1.530 CPU sec, 1.534 real sec...
[M::mem_process_seqs] Processed 676102 reads in 127.801 CPU sec, 4.033 real sec
[M::mem_process_seqs] Processed 676300 reads in 83.338 CPU sec, 2.632 real sec
sorting block 106, #reads = 1075895 mem = 536884406 peak = 1202147328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282024, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 351, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (365.98, 163.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675702 sequences (100000273 bp) in 2.166 CPU sec, 2.204 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281135, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (362.68, 160.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675316 sequences (100000275 bp) in 1.550 CPU sec, 1.546 real sec...
[M::mem_process_seqs] Processed 675820 reads in 132.664 CPU sec, 4.191 real sec
[M::mem_process_seqs] Processed 675600 reads in 86.124 CPU sec, 2.721 real sec
sorting block 107, #reads = 1076602 mem = 536878864 peak = 1202147328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281175, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (362.71, 160.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675052 sequences (100000108 bp) in 2.185 CPU sec, 2.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281665, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 349, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (363.96, 161.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675096 sequences (100000101 bp) in 1.530 CPU sec, 1.532 real sec...
[M::mem_process_seqs] Processed 675702 reads in 134.451 CPU sec, 4.250 real sec
sorting block 108, #reads = 1076054 mem = 536878360 peak = 1202147328
[M::mem_process_seqs] Processed 675316 reads in 86.845 CPU sec, 2.741 real sec
sorting block 109, #reads = 1074646 mem = 536887756 peak = 1202147328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281355, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (365.30, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675106 sequences (100000040 bp) in 2.172 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281339, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (363.30, 159.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675212 sequences (100000065 bp) in 1.530 CPU sec, 1.538 real sec...
[M::mem_process_seqs] Processed 675052 reads in 130.793 CPU sec, 4.128 real sec
[M::mem_process_seqs] Processed 675096 reads in 83.867 CPU sec, 2.644 real sec
sorting block 110, #reads = 1075553 mem = 536879465 peak = 1202147328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281740, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (363.21, 160.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675386 sequences (100000005 bp) in 2.237 CPU sec, 2.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281345, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (364.22, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675382 sequences (100000190 bp) in 1.580 CPU sec, 1.579 real sec...
[M::mem_process_seqs] Processed 675106 reads in 129.085 CPU sec, 4.074 real sec
[M::mem_process_seqs] Processed 675212 reads in 83.682 CPU sec, 2.641 real sec
sorting block 111, #reads = 1075314 mem = 536879276 peak = 1202147328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281181, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (362.34, 159.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675590 sequences (100000279 bp) in 2.234 CPU sec, 2.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280920, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (361.46, 159.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675516 sequences (100000028 bp) in 1.530 CPU sec, 1.534 real sec...
[M::mem_process_seqs] Processed 675386 reads in 131.664 CPU sec, 4.158 real sec
sorting block 112, #reads = 1075678 mem = 536882558 peak = 1202147328
[M::mem_process_seqs] Processed 675382 reads in 84.893 CPU sec, 2.678 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281772, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (359.52, 158.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675392 sequences (100000253 bp) in 2.166 CPU sec, 2.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281463, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (361.69, 160.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675206 sequences (100000222 bp) in 1.520 CPU sec, 1.516 real sec...
[M::mem_process_seqs] Processed 675590 reads in 130.719 CPU sec, 4.129 real sec
sorting block 113, #reads = 1074746 mem = 536880451 peak = 1202147328
[M::mem_process_seqs] Processed 675516 reads in 84.078 CPU sec, 2.653 real sec
sorting block 114, #reads = 1074686 mem = 536881740 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281515, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (361.29, 159.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675240 sequences (100000020 bp) in 2.136 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281491, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (362.46, 159.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674926 sequences (100000138 bp) in 1.560 CPU sec, 1.551 real sec...
[M::mem_process_seqs] Processed 675392 reads in 131.609 CPU sec, 4.159 real sec
[M::mem_process_seqs] Processed 675206 reads in 84.787 CPU sec, 2.674 real sec
sorting block 115, #reads = 1075397 mem = 536878732 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281372, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (362.91, 159.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675034 sequences (100000232 bp) in 2.022 CPU sec, 2.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281100, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (362.25, 159.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675070 sequences (100000170 bp) in 1.520 CPU sec, 1.522 real sec...
[M::mem_process_seqs] Processed 675240 reads in 129.626 CPU sec, 4.096 real sec
[M::mem_process_seqs] Processed 674926 reads in 83.692 CPU sec, 2.642 real sec
sorting block 116, #reads = 1074845 mem = 536880202 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281691, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (365.00, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680462 sequences (100000207 bp) in 2.169 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282066, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (364.37, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682278 sequences (100000148 bp) in 1.580 CPU sec, 1.580 real sec...
[M::mem_process_seqs] Processed 675034 reads in 132.022 CPU sec, 4.168 real sec
sorting block 117, #reads = 1074506 mem = 536879453 peak = 1202282496
[M::mem_process_seqs] Processed 675070 reads in 86.121 CPU sec, 2.716 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283512, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (329.70, 151.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681474 sequences (100000276 bp) in 2.196 CPU sec, 2.218 real sec...
[M::process] read 680894 sequences (100000274 bp) in 1.570 CPU sec, 1.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283475, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 299, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (318.41, 146.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680462 reads in 144.627 CPU sec, 4.574 real sec
sorting block 118, #reads = 1072542 mem = 536878066 peak = 1202282496
[M::mem_process_seqs] Processed 682278 reads in 92.997 CPU sec, 2.945 real sec
sorting block 119, #reads = 1071723 mem = 536879185 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284004, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (324.17, 149.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681052 sequences (100000200 bp) in 2.193 CPU sec, 2.228 real sec...
[M::process] read 680724 sequences (100000149 bp) in 1.600 CPU sec, 1.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283949, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 310, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (328.52, 151.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681474 reads in 141.134 CPU sec, 4.467 real sec
[M::mem_process_seqs] Processed 680894 reads in 91.244 CPU sec, 2.882 real sec
sorting block 120, #reads = 1073254 mem = 536883869 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283926, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (323.84, 146.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679632 sequences (100000126 bp) in 2.248 CPU sec, 2.272 real sec...
[M::process] read 679728 sequences (100000267 bp) in 1.540 CPU sec, 1.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283690, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (327.23, 147.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681052 reads in 142.046 CPU sec, 4.491 real sec
[M::mem_process_seqs] Processed 680724 reads in 90.966 CPU sec, 2.882 real sec
sorting block 121, #reads = 1073558 mem = 536880936 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283099, 16, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (331.21, 148.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (804, 989, 989)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 1359)
[M::mem_pestat] mean and std.dev: (976.62, 180.63)
[M::mem_pestat] low and high boundaries for proper pairs: (249, 1699)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 679120 sequences (100000227 bp) in 2.044 CPU sec, 2.070 real sec...
[M::process] read 678694 sequences (100000097 bp) in 1.600 CPU sec, 1.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283715, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 318, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (333.29, 150.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679632 reads in 139.335 CPU sec, 4.406 real sec
sorting block 122, #reads = 1072392 mem = 536878772 peak = 1202282496
[M::mem_process_seqs] Processed 679728 reads in 89.977 CPU sec, 2.845 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283383, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 322, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (337.07, 151.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678294 sequences (100000051 bp) in 1.994 CPU sec, 2.012 real sec...
[M::process] read 677698 sequences (100000237 bp) in 1.520 CPU sec, 1.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283088, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (340.75, 153.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679120 reads in 136.406 CPU sec, 4.318 real sec
sorting block 123, #reads = 1072344 mem = 536878900 peak = 1202282496
[M::mem_process_seqs] Processed 678694 reads in 88.009 CPU sec, 2.793 real sec
sorting block 124, #reads = 1073331 mem = 536879077 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282829, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (341.53, 152.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677504 sequences (100000106 bp) in 1.989 CPU sec, 2.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282761, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677436 sequences (100000020 bp) in 1.540 CPU sec, 1.547 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (346.00, 153.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678294 reads in 133.472 CPU sec, 4.227 real sec
[M::mem_process_seqs] Processed 677698 reads in 85.804 CPU sec, 2.712 real sec
sorting block 125, #reads = 1074373 mem = 536881155 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282608, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (347.44, 154.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677264 sequences (100000138 bp) in 2.141 CPU sec, 2.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282322, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (348.93, 155.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676826 sequences (100000277 bp) in 1.520 CPU sec, 1.511 real sec...
[M::mem_process_seqs] Processed 677504 reads in 131.414 CPU sec, 4.152 real sec
[M::mem_process_seqs] Processed 677436 reads in 84.442 CPU sec, 2.663 real sec
sorting block 126, #reads = 1075373 mem = 536883516 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282393, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (350.37, 156.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676824 sequences (100000054 bp) in 2.023 CPU sec, 2.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282190, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.97, 157.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676582 sequences (100000149 bp) in 1.510 CPU sec, 1.517 real sec...
[M::mem_process_seqs] Processed 677264 reads in 131.327 CPU sec, 4.153 real sec
sorting block 127, #reads = 1074990 mem = 536878642 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281694, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (356.14, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676694 sequences (100000031 bp) in 1.903 CPU sec, 1.909 real sec...
[M::mem_process_seqs] Processed 676824 reads in 73.278 CPU sec, 2.305 real sec
[M::mem_process_seqs] Processed 676826 reads in 173.413 CPU sec, 5.486 real sec
sorting block 128, #reads = 1074338 mem = 536878397 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282308, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (356.86, 159.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676040 sequences (100000271 bp) in 2.225 CPU sec, 2.255 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282558, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (350.49, 155.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676582 reads in 149.145 CPU sec, 4.718 real sec
[M::process] read 676100 sequences (100000245 bp) in 1.530 CPU sec, 1.538 real sec...
sorting block 129, #reads = 1074552 mem = 536879617 peak = 1202282496
[M::mem_process_seqs] Processed 676694 reads in 89.627 CPU sec, 2.823 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281947, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (359.51, 159.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675622 sequences (100000076 bp) in 2.078 CPU sec, 2.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282451, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (359.14, 159.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (676, 791, 4651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12601)
[M::mem_pestat] mean and std.dev: (2067.55, 1960.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16576)
[M::mem_pestat] skip orientation RR
[M::process] read 675552 sequences (100000186 bp) in 1.620 CPU sec, 1.626 real sec...
[M::mem_process_seqs] Processed 676040 reads in 140.175 CPU sec, 4.435 real sec
sorting block 130, #reads = 1074962 mem = 536879267 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281766, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (362.73, 161.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675508 sequences (100000039 bp) in 1.993 CPU sec, 1.997 real sec...
[M::mem_process_seqs] Processed 675622 reads in 79.963 CPU sec, 2.522 real sec
sorting block 131, #reads = 1074568 mem = 536877776 peak = 1202282496
[M::mem_process_seqs] Processed 676100 reads in 199.695 CPU sec, 6.323 real sec
[M::process] read 675320 sequences (100000099 bp) in 2.210 CPU sec, 2.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281505, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (363.64, 161.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675508 reads in 79.119 CPU sec, 2.472 real sec
sorting block 132, #reads = 1074955 mem = 536879693 peak = 1202282496
[M::process] read 675524 sequences (100000078 bp) in 1.889 CPU sec, 1.904 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281943, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (363.03, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281390, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 350, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (366.48, 163.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675552 reads in 238.441 CPU sec, 7.533 real sec
[M::process] read 675332 sequences (100000173 bp) in 1.970 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 675320 reads in 110.134 CPU sec, 3.471 real sec
sorting block 133, #reads = 1074623 mem = 536879535 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281321, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (363.84, 163.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675488 sequences (100000025 bp) in 2.155 CPU sec, 2.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281833, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (365.02, 163.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675166 sequences (100000003 bp) in 1.710 CPU sec, 1.713 real sec...
[M::mem_process_seqs] Processed 675524 reads in 141.662 CPU sec, 4.481 real sec
sorting block 134, #reads = 1076169 mem = 536879102 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281588, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 350, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (366.79, 164.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675634 sequences (100000117 bp) in 1.777 CPU sec, 1.781 real sec...
[M::mem_process_seqs] Processed 675488 reads in 78.694 CPU sec, 2.489 real sec
[M::mem_process_seqs] Processed 675332 reads in 194.134 CPU sec, 6.134 real sec
sorting block 135, #reads = 1075513 mem = 536877538 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281734, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 351, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (367.71, 164.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675426 sequences (100000135 bp) in 2.441 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281391, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (364.35, 163.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675166 reads in 160.036 CPU sec, 5.067 real sec
[M::mem_process_seqs] Processed 675634 reads in 97.978 CPU sec, 3.073 real sec
sorting block 136, #reads = 1075669 mem = 536877317 peak = 1202282496
[M::process] read 675204 sequences (100000148 bp) in 1.952 CPU sec, 1.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281879, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 353, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (370.05, 167.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675158 sequences (100000270 bp) in 2.223 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281411, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 354, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (371.67, 168.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678954 sequences (100000296 bp) in 1.690 CPU sec, 1.693 real sec...
[M::mem_process_seqs] Processed 675426 reads in 144.251 CPU sec, 4.555 real sec
sorting block 137, #reads = 1073111 mem = 536879045 peak = 1202282496
[M::mem_process_seqs] Processed 675204 reads in 94.665 CPU sec, 2.980 real sec
sorting block 138, #reads = 1074737 mem = 536877700 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281410, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 352, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (370.56, 168.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680128 sequences (100000115 bp) in 2.406 CPU sec, 2.447 real sec...
[M::process] read 680548 sequences (100000119 bp) in 1.700 CPU sec, 1.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282605, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (342.46, 158.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675158 reads in 163.082 CPU sec, 5.159 real sec
[M::mem_process_seqs] Processed 678954 reads in 110.192 CPU sec, 3.495 real sec
sorting block 139, #reads = 1067675 mem = 536880383 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283095, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (334.70, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680296 sequences (100000042 bp) in 2.393 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283104, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 314, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (334.01, 156.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680128 reads in 146.608 CPU sec, 4.646 real sec
[M::process] read 679792 sequences (100000032 bp) in 1.836 CPU sec, 1.842 real sec...
[M::mem_process_seqs] Processed 680548 reads in 96.398 CPU sec, 3.035 real sec
sorting block 140, #reads = 1074526 mem = 536878645 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283439, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (333.99, 155.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678858 sequences (100000014 bp) in 2.216 CPU sec, 2.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283520, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (336.32, 155.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678870 sequences (100000219 bp) in 1.650 CPU sec, 1.649 real sec...
[M::mem_process_seqs] Processed 680296 reads in 144.349 CPU sec, 4.566 real sec
sorting block 141, #reads = 1077325 mem = 536880275 peak = 1202282496
[M::mem_process_seqs] Processed 679792 reads in 91.372 CPU sec, 2.883 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282424, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (343.12, 159.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678676 sequences (100000097 bp) in 2.039 CPU sec, 2.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282490, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (345.96, 160.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678000 sequences (100000201 bp) in 1.590 CPU sec, 1.590 real sec...
[M::mem_process_seqs] Processed 678858 reads in 133.034 CPU sec, 4.204 real sec
sorting block 142, #reads = 1077665 mem = 536877569 peak = 1202282496
[M::mem_process_seqs] Processed 678870 reads in 87.136 CPU sec, 2.749 real sec
sorting block 143, #reads = 1077945 mem = 536881511 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283149, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (345.52, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677126 sequences (100000179 bp) in 2.207 CPU sec, 2.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282517, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (347.37, 158.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677582 sequences (100000083 bp) in 1.620 CPU sec, 1.618 real sec...
[M::mem_process_seqs] Processed 678676 reads in 135.263 CPU sec, 4.268 real sec
[M::mem_process_seqs] Processed 678000 reads in 87.684 CPU sec, 2.762 real sec
sorting block 144, #reads = 1079549 mem = 536878582 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282239, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (349.59, 157.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677422 sequences (100000246 bp) in 1.929 CPU sec, 1.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282323, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (348.96, 159.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677242 sequences (100000125 bp) in 1.610 CPU sec, 1.608 real sec...
[M::mem_process_seqs] Processed 677126 reads in 132.712 CPU sec, 4.196 real sec
[M::mem_process_seqs] Processed 677582 reads in 86.722 CPU sec, 2.737 real sec
sorting block 145, #reads = 1078395 mem = 536881013 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282934, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (350.26, 158.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677068 sequences (100000212 bp) in 2.247 CPU sec, 2.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282162, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (351.14, 159.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677046 sequences (100000131 bp) in 1.634 CPU sec, 1.629 real sec...
[M::mem_process_seqs] Processed 677422 reads in 134.381 CPU sec, 4.241 real sec
sorting block 146, #reads = 1078218 mem = 536877897 peak = 1202282496
[M::mem_process_seqs] Processed 677242 reads in 86.822 CPU sec, 2.738 real sec
sorting block 147, #reads = 1077958 mem = 536877886 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282436, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (350.98, 157.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677278 sequences (100000215 bp) in 2.198 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281828, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (350.03, 156.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676708 sequences (100000206 bp) in 1.610 CPU sec, 1.614 real sec...
[M::mem_process_seqs] Processed 677068 reads in 136.929 CPU sec, 4.326 real sec
[M::mem_process_seqs] Processed 677046 reads in 88.522 CPU sec, 2.791 real sec
sorting block 148, #reads = 1078004 mem = 536879890 peak = 1202282496
[M::process] read 676600 sequences (100000187 bp) in 2.410 CPU sec, 2.447 real sec...
[M::process] read 677010 sequences (100000266 bp) in 1.640 CPU sec, 1.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281911, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (352.38, 157.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676600 reads in 71.732 CPU sec, 2.340 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282250, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (351.92, 157.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676708 reads in 147.420 CPU sec, 5.112 real sec
sorting block 149, #reads = 1077812 mem = 536877814 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282043, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (349.64, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677004 sequences (100000284 bp) in 2.454 CPU sec, 2.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282016, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.45, 158.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677278 reads in 291.278 CPU sec, 9.650 real sec
sorting block 150, #reads = 1076660 mem = 536885371 peak = 1202282496
[M::process] read 677590 sequences (100000280 bp) in 2.026 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282163, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.71, 158.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677004 reads in 79.044 CPU sec, 2.485 real sec
[M::process] read 677264 sequences (100000237 bp) in 1.686 CPU sec, 1.692 real sec...
[M::mem_process_seqs] Processed 677010 reads in 213.487 CPU sec, 6.727 real sec
sorting block 151, #reads = 1076705 mem = 536880581 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282185, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (343.77, 155.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677324 sequences (100000144 bp) in 2.120 CPU sec, 2.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282352, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (349.38, 157.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677590 reads in 157.135 CPU sec, 4.948 real sec
[M::process] read 677700 sequences (100000033 bp) in 1.658 CPU sec, 1.658 real sec...
[M::mem_process_seqs] Processed 677264 reads in 92.540 CPU sec, 2.912 real sec
sorting block 152, #reads = 1076652 mem = 536880604 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281780, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (347.67, 156.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676688 sequences (100000226 bp) in 2.307 CPU sec, 2.344 real sec...
[M::process] read 676228 sequences (100000143 bp) in 1.580 CPU sec, 1.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282476, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (347.38, 157.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677324 reads in 144.052 CPU sec, 4.552 real sec
sorting block 153, #reads = 1076504 mem = 536879311 peak = 1202282496
[M::mem_process_seqs] Processed 677700 reads in 93.529 CPU sec, 2.961 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281991, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (357.85, 162.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675956 sequences (100000082 bp) in 2.096 CPU sec, 2.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282440, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 675892 sequences (100000054 bp) in 1.670 CPU sec, 1.669 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (234, 344, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (361.85, 164.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676688 reads in 141.691 CPU sec, 4.477 real sec
sorting block 154, #reads = 1075579 mem = 536877467 peak = 1202282496
[M::mem_process_seqs] Processed 676228 reads in 92.084 CPU sec, 2.917 real sec
sorting block 155, #reads = 1076588 mem = 536880429 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281617, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (362.46, 163.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675778 sequences (100000292 bp) in 2.514 CPU sec, 2.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281800, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (362.19, 162.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675684 sequences (100000228 bp) in 1.630 CPU sec, 1.629 real sec...
[M::mem_process_seqs] Processed 675956 reads in 144.450 CPU sec, 4.561 real sec
[M::mem_process_seqs] Processed 675892 reads in 93.149 CPU sec, 2.941 real sec
sorting block 156, #reads = 1074501 mem = 536882802 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281709, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (363.28, 163.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675372 sequences (100000025 bp) in 2.405 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281762, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (364.26, 163.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675544 sequences (100000196 bp) in 1.660 CPU sec, 1.659 real sec...
[M::mem_process_seqs] Processed 675778 reads in 142.197 CPU sec, 4.486 real sec
[M::mem_process_seqs] Processed 675684 reads in 92.611 CPU sec, 2.914 real sec
sorting block 157, #reads = 1075320 mem = 536878311 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282123, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 350, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (366.38, 164.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676542 sequences (100000216 bp) in 2.226 CPU sec, 2.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281281, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (366.91, 166.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675638 sequences (100000164 bp) in 1.680 CPU sec, 1.689 real sec...
[M::mem_process_seqs] Processed 675372 reads in 141.816 CPU sec, 4.478 real sec
sorting block 158, #reads = 1075577 mem = 536876888 peak = 1202282496
[M::mem_process_seqs] Processed 675544 reads in 92.779 CPU sec, 2.927 real sec
sorting block 159, #reads = 1075034 mem = 536879888 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281972, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (357.37, 164.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676164 sequences (100000242 bp) in 2.247 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281778, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (366.74, 167.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681728 sequences (100000028 bp) in 1.720 CPU sec, 1.728 real sec...
[M::mem_process_seqs] Processed 676542 reads in 148.994 CPU sec, 4.708 real sec
[M::mem_process_seqs] Processed 675638 reads in 97.491 CPU sec, 3.085 real sec
sorting block 160, #reads = 1073654 mem = 536878492 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280893, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 345, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (363.89, 167.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682344 sequences (100000138 bp) in 2.359 CPU sec, 2.398 real sec...
[M::process] read 682074 sequences (100000256 bp) in 1.680 CPU sec, 1.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283521, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 306, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (325.80, 152.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676164 reads in 168.642 CPU sec, 5.337 real sec
[M::mem_process_seqs] Processed 681728 reads in 111.339 CPU sec, 3.538 real sec
sorting block 161, #reads = 1065898 mem = 536881386 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283545, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 683050 sequences (100000082 bp) in 2.110 CPU sec, 2.143 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (203, 301, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (318.94, 146.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681768 sequences (100000139 bp) in 1.710 CPU sec, 1.707 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284059, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 302, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (321.05, 148.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682344 reads in 156.070 CPU sec, 4.937 real sec
[M::mem_process_seqs] Processed 682074 reads in 101.062 CPU sec, 3.205 real sec
sorting block 162, #reads = 1072026 mem = 536880085 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284390, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 297, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (315.57, 145.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682074 sequences (100000235 bp) in 2.452 CPU sec, 2.502 real sec...
[M::process] read 681528 sequences (100000142 bp) in 1.710 CPU sec, 1.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284302, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 304, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (320.03, 145.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683050 reads in 159.358 CPU sec, 5.040 real sec
sorting block 163, #reads = 1073393 mem = 536877922 peak = 1202282496
[M::mem_process_seqs] Processed 681768 reads in 102.229 CPU sec, 3.242 real sec
sorting block 164, #reads = 1072094 mem = 536878194 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284017, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 301, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (319.29, 146.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681316 sequences (100000085 bp) in 2.424 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283806, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (322.09, 146.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680024 sequences (100000182 bp) in 1.830 CPU sec, 1.835 real sec...
[M::mem_process_seqs] Processed 682074 reads in 157.022 CPU sec, 4.956 real sec
[M::mem_process_seqs] Processed 681528 reads in 102.644 CPU sec, 3.244 real sec
sorting block 165, #reads = 1073991 mem = 536878963 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283700, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 307, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (322.45, 144.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679294 sequences (100000265 bp) in 2.323 CPU sec, 2.361 real sec...
[M::process] read 678640 sequences (100000160 bp) in 1.650 CPU sec, 1.647 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283337, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 314, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (329.19, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681316 reads in 154.982 CPU sec, 4.899 real sec
[M::mem_process_seqs] Processed 680024 reads in 98.295 CPU sec, 3.119 real sec
sorting block 166, #reads = 1070732 mem = 536880842 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282740, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 321, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (336.10, 151.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678444 sequences (100000293 bp) in 2.101 CPU sec, 2.138 real sec...
[M::process] read 678194 sequences (100000024 bp) in 1.670 CPU sec, 1.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282449, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 324, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (339.51, 152.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679294 reads in 148.142 CPU sec, 4.688 real sec
sorting block 167, #reads = 1074044 mem = 536878648 peak = 1202282496
[M::mem_process_seqs] Processed 678640 reads in 94.548 CPU sec, 2.994 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283168, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (342.82, 153.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678138 sequences (100000259 bp) in 2.401 CPU sec, 2.435 real sec...
[M::process] read 677896 sequences (100000096 bp) in 1.640 CPU sec, 1.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282684, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (343.39, 154.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678444 reads in 147.185 CPU sec, 4.650 real sec
sorting block 168, #reads = 1074534 mem = 536879579 peak = 1202282496
[M::mem_process_seqs] Processed 678194 reads in 95.461 CPU sec, 3.016 real sec
sorting block 169, #reads = 1074501 mem = 536880291 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282873, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (346.22, 156.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677712 sequences (100000260 bp) in 2.518 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282338, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (345.71, 154.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677428 sequences (100000063 bp) in 1.660 CPU sec, 1.663 real sec...
[M::mem_process_seqs] Processed 678138 reads in 147.587 CPU sec, 4.658 real sec
[M::mem_process_seqs] Processed 677896 reads in 95.182 CPU sec, 3.000 real sec
sorting block 170, #reads = 1075140 mem = 536878115 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282884, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (349.42, 157.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677370 sequences (100000192 bp) in 2.337 CPU sec, 2.376 real sec...
[M::process] read 676936 sequences (100000288 bp) in 1.630 CPU sec, 1.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281998, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (351.95, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677712 reads in 146.820 CPU sec, 4.638 real sec
[M::mem_process_seqs] Processed 677428 reads in 94.681 CPU sec, 2.996 real sec
sorting block 171, #reads = 1074464 mem = 536877476 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282303, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.48, 158.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676952 sequences (100000286 bp) in 2.026 CPU sec, 2.053 real sec...
[M::process] read 676524 sequences (100000177 bp) in 1.630 CPU sec, 1.638 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281965, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (353.85, 159.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677370 reads in 146.516 CPU sec, 4.639 real sec
sorting block 172, #reads = 1074914 mem = 536878981 peak = 1202282496
[M::mem_process_seqs] Processed 676936 reads in 93.842 CPU sec, 2.976 real sec
sorting block 173, #reads = 1074313 mem = 536876099 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281695, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (355.83, 160.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676568 sequences (100000219 bp) in 2.426 CPU sec, 2.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282156, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 342, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (355.75, 157.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676198 sequences (100000190 bp) in 1.610 CPU sec, 1.608 real sec...
[M::mem_process_seqs] Processed 676952 reads in 145.908 CPU sec, 4.609 real sec
[M::mem_process_seqs] Processed 676524 reads in 93.076 CPU sec, 2.937 real sec
sorting block 174, #reads = 1076153 mem = 536878459 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281830, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (352.50, 155.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676134 sequences (100000028 bp) in 2.102 CPU sec, 2.143 real sec...
[M::process] read 675744 sequences (100000166 bp) in 1.640 CPU sec, 1.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281980, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 345, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (359.12, 159.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676568 reads in 143.175 CPU sec, 4.528 real sec
[M::mem_process_seqs] Processed 676198 reads in 92.604 CPU sec, 2.924 real sec
sorting block 175, #reads = 1075649 mem = 536879987 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282071, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (360.17, 159.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675284 sequences (100000279 bp) in 2.265 CPU sec, 2.303 real sec...
[M::process] read 675346 sequences (100000234 bp) in 1.600 CPU sec, 1.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281807, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 349, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (363.71, 160.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676134 reads in 142.092 CPU sec, 4.490 real sec
sorting block 176, #reads = 1075600 mem = 536879870 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281189, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 353, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (366.01, 160.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675264 sequences (100000236 bp) in 2.044 CPU sec, 2.048 real sec...
[M::mem_process_seqs] Processed 675284 reads in 78.642 CPU sec, 2.479 real sec
[M::mem_process_seqs] Processed 675744 reads in 185.655 CPU sec, 5.868 real sec
sorting block 177, #reads = 1076168 mem = 536878277 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281655, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (365.36, 160.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675602 sequences (100000081 bp) in 2.277 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281704, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (364.68, 160.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675346 reads in 153.080 CPU sec, 4.833 real sec
[M::process] read 675296 sequences (100000106 bp) in 1.609 CPU sec, 1.611 real sec...
[M::mem_process_seqs] Processed 675264 reads in 90.744 CPU sec, 2.855 real sec
sorting block 178, #reads = 1076115 mem = 536879930 peak = 1202282496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281281, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (359.88, 158.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674696 sequences (100000134 bp) in 2.477 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281303, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (363.23, 160.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675602 reads in 141.821 CPU sec, 4.475 real sec
[M::process] read 675172 sequences (100000214 bp) in 1.699 CPU sec, 1.701 real sec...
sorting block 179, #reads = 1075485 mem = 536877908 peak = 1202282496
[M::mem_process_seqs] Processed 675296 reads in 91.221 CPU sec, 2.862 real sec
[M::process] read 178570 sequences (26392913 bp) in 0.944 CPU sec, 0.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281651, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (363.95, 159.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281695, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (362.84, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 74593, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 336, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (350.85, 155.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674696 reads in 151.000 CPU sec, 4.867 real sec
sorting block 180, #reads = 1075754 mem = 536879632 peak = 1202282496
[M::mem_process_seqs] Processed 675172 reads in 106.158 CPU sec, 3.427 real sec
[M::mem_process_seqs] Processed 178570 reads in 110.755 CPU sec, 3.583 real sec
sorting block 181, #reads = 1076245 mem = 536880673 peak = 1216688128
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 195677468
overall: 69853454336 mem 23509.171 user 469.510 sys 829.265 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_bR4-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tLB:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 829.296 sec; CPU: 23978.684 sec
sorting block 182, #reads = 398168 mem = 199474672 peak = 1216688128
uniq header count 1
merging 183 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 11016851314
output reads: 196491254
bam_mem_sort: 183 calls 86.647 user 1.374 sys 89.275 real
bam_write: 183 calls 121.265 user 4.757 sys 140.837 real
execute: 1 calls 51.286 user 1.615 sys 909.792 real
merge_files: 1 calls 51.255 user 1.565 sys 79.976 real
parse_chunk: 2918 calls 146.327 user 31.055 sys 186.613 real
read_chunk: 7862 calls 74.122 user 48.476 sys 742.603 real
sort_block: 1 calls 46.663 user 47.934 sys 707.186 real
write_chunk: 4942 calls 50.822 user 1.122 sys 78.767 real
overall: 3409473536 mem 3075.729 user 105.203 sys 909.814 real
ls: cannot access '*.cram': No such file or directory