cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1695.641 CPU sec, 1075.903 real sec
[M::process] read 687818 sequences (100000205 bp) in 0.981 CPU sec, 0.990 real sec...
[M::process] read 685386 sequences (100000060 bp) in 1.120 CPU sec, 1.122 real sec...
[M::process] read 684512 sequences (100000263 bp) in 1.138 CPU sec, 1.170 real sec...
[M::process] read 683608 sequences (100000241 bp) in 1.117 CPU sec, 1.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283712, 3, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (333.59, 160.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 310, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (328.06, 162.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 274, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321)
[M::mem_pestat] mean and std.dev: (385.00, 285.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1684)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685386 reads in 76.745 CPU sec, 2.488 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284695, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (273, 370, 872)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2070)
[M::mem_pestat] mean and std.dev: (662.95, 622.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3152)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 295, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (317.76, 160.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 348, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (334.12, 143.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683512 sequences (100000282 bp) in 1.117 CPU sec, 1.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283152, 9, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 263, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (269.92, 128.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 316, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (333.76, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 348, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (301.25, 123.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687818 reads in 217.304 CPU sec, 6.957 real sec
[M::mem_process_seqs] Processed 684512 reads in 99.121 CPU sec, 3.180 real sec
sorting block 0, #reads = 1081527 mem = 536880291 peak = 608350208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283546, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 333, 860)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2154)
[M::mem_pestat] mean and std.dev: (564.33, 422.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2801)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 321, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (338.08, 165.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (118, 247, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532)
[M::mem_pestat] mean and std.dev: (189.78, 69.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682692 sequences (100000227 bp) in 1.761 CPU sec, 1.918 real sec...
[M::process] read 682372 sequences (100000088 bp) in 1.220 CPU sec, 1.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282708, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 263, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (252.40, 89.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (341.53, 167.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 312, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469)
[M::mem_pestat] mean and std.dev: (379.43, 221.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1893)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683608 reads in 139.978 CPU sec, 4.499 real sec
sorting block 1, #reads = 1080948 mem = 536879385 peak = 822894592
[M::mem_process_seqs] Processed 683512 reads in 88.617 CPU sec, 2.873 real sec
sorting block 2, #reads = 1080225 mem = 536880737 peak = 977436672
[M::process] read 682550 sequences (100000269 bp) in 1.676 CPU sec, 1.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282572, 1, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 320, 1085)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2837)
[M::mem_pestat] mean and std.dev: (570.22, 531.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3713)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 328, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (343.66, 167.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (311, 349, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (418.23, 125.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682770 sequences (100000124 bp) in 1.250 CPU sec, 1.253 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282250, 10, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 478, 660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1508)
[M::mem_pestat] mean and std.dev: (364.93, 185.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1932)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (340.59, 165.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (302, 1319, 3463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9785)
[M::mem_pestat] mean and std.dev: (1627.60, 1289.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12946)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 262, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 477)
[M::mem_pestat] mean and std.dev: (244.23, 62.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 562)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682692 reads in 141.094 CPU sec, 4.500 real sec
[M::process] read 682548 sequences (100000237 bp) in 1.489 CPU sec, 1.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282056, 7, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 222, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (218.83, 76.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (336.72, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (289, 314, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (355.47, 179.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682550 reads in 78.512 CPU sec, 2.500 real sec
sorting block 3, #reads = 1078704 mem = 536879830 peak = 977436672
[M::mem_process_seqs] Processed 682372 reads in 190.734 CPU sec, 6.098 real sec
sorting block 4, #reads = 1079418 mem = 536877229 peak = 977436672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 281969, 1, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (332, 446, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1547)
[M::mem_pestat] mean and std.dev: (434.71, 208.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1952)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 319, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (334.33, 160.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 346, 785)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985)
[M::mem_pestat] mean and std.dev: (409.80, 264.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2585)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681866 sequences (100000232 bp) in 1.688 CPU sec, 1.792 real sec...
[M::process] read 685946 sequences (100000132 bp) in 1.260 CPU sec, 1.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282793, 8, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 297, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196)
[M::mem_pestat] mean and std.dev: (337.45, 223.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1544)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (336.04, 161.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 323, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (286.25, 128.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682770 reads in 155.280 CPU sec, 4.956 real sec
[M::mem_process_seqs] Processed 682548 reads in 89.092 CPU sec, 2.840 real sec
sorting block 5, #reads = 1077875 mem = 536880825 peak = 977436672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282524, 8, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (255, 368, 1265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3285)
[M::mem_pestat] mean and std.dev: (472.94, 452.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4295)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (341.54, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 313, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (283.22, 170.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685576 sequences (100000233 bp) in 1.990 CPU sec, 2.116 real sec...
[M::process] read 684562 sequences (100000128 bp) in 1.380 CPU sec, 1.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283347, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 368, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (317.46, 176.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 307, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (326.71, 163.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 367, 687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1721)
[M::mem_pestat] mean and std.dev: (434.06, 350.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2238)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681866 reads in 142.542 CPU sec, 4.546 real sec
[M::mem_process_seqs] Processed 685946 reads in 89.495 CPU sec, 2.847 real sec
sorting block 6, #reads = 1077118 mem = 536879633 peak = 977436672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283389, 4, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 240, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (257.05, 103.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 311, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (329.12, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 346, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (298.52, 125.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683874 sequences (100000189 bp) in 1.788 CPU sec, 1.917 real sec...
[M::process] read 683354 sequences (100000048 bp) in 1.300 CPU sec, 1.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283533, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 291, 601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429)
[M::mem_pestat] mean and std.dev: (483.30, 424.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2183)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (334.27, 164.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 414, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117)
[M::mem_pestat] mean and std.dev: (326.08, 157.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1429)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685576 reads in 139.858 CPU sec, 4.468 real sec
[M::mem_process_seqs] Processed 684562 reads in 87.183 CPU sec, 2.779 real sec
sorting block 7, #reads = 1080807 mem = 536879185 peak = 977436672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283121, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 195, 826)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2114)
[M::mem_pestat] mean and std.dev: (432.95, 390.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2758)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 323, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (338.93, 165.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 266, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (237.81, 66.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 658)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682444 sequences (100000164 bp) in 2.118 CPU sec, 2.266 real sec...
[M::process] read 682278 sequences (100000301 bp) in 1.350 CPU sec, 1.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283441, 7, 28)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (340.92, 166.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 325, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (349.27, 184.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1376)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683874 reads in 140.226 CPU sec, 4.471 real sec
sorting block 8, #reads = 1081507 mem = 536876700 peak = 977436672
[M::mem_process_seqs] Processed 683354 reads in 86.706 CPU sec, 2.758 real sec
sorting block 9, #reads = 1081465 mem = 536881310 peak = 977436672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282576, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 264, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (300.35, 133.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (344.42, 166.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (285, 432, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (413.53, 154.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682436 sequences (100000261 bp) in 1.874 CPU sec, 2.003 real sec...
[M::process] read 682622 sequences (100000258 bp) in 1.360 CPU sec, 1.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282469, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 246, 635)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625)
[M::mem_pestat] mean and std.dev: (315.88, 182.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2120)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (342.39, 165.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 232, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (294.65, 185.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682444 reads in 141.977 CPU sec, 4.541 real sec
[M::mem_process_seqs] Processed 682278 reads in 88.931 CPU sec, 2.830 real sec
sorting block 10, #reads = 1079297 mem = 536879242 peak = 977436672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282495, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 531, 819)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1951)
[M::mem_pestat] mean and std.dev: (477.80, 251.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2517)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (338.23, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (309.69, 134.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682344 sequences (100000199 bp) in 2.203 CPU sec, 2.354 real sec...
[M::process] read 682388 sequences (100000213 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282466, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 218, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (283.69, 144.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (336.45, 161.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 261, 807)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1993)
[M::mem_pestat] mean and std.dev: (338.06, 224.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2586)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682436 reads in 143.092 CPU sec, 4.562 real sec
[M::mem_process_seqs] Processed 682622 reads in 89.572 CPU sec, 2.850 real sec
sorting block 11, #reads = 1079083 mem = 536882562 peak = 977436672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282802, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 331, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150)
[M::mem_pestat] mean and std.dev: (402.42, 223.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1452)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 322, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (337.94, 162.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 320, 748)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832)
[M::mem_pestat] mean and std.dev: (399.77, 253.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2374)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684724 sequences (100000001 bp) in 2.048 CPU sec, 2.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282481, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 430, 1210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3264)
[M::mem_pestat] mean and std.dev: (491.40, 572.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4291)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 687372 sequences (100000011 bp) in 1.380 CPU sec, 1.376 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (205, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (339.51, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (289, 328, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (387.73, 190.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682344 reads in 142.985 CPU sec, 4.565 real sec
sorting block 12, #reads = 1077880 mem = 536879295 peak = 977436672
[M::mem_process_seqs] Processed 682388 reads in 89.171 CPU sec, 2.834 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283384, 3, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 184, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (241.62, 147.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (331.95, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (374, 452, 788)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1616)
[M::mem_pestat] mean and std.dev: (529.14, 291.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2030)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 13, #reads = 1077742 mem = 536881166 peak = 977436672
[M::process] read 686188 sequences (100000121 bp) in 2.313 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284247, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 306, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (347.32, 174.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 685326 sequences (100000048 bp) in 1.440 CPU sec, 1.432 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (188, 300, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (320.92, 161.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 327, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (354.11, 201.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684724 reads in 144.047 CPU sec, 4.589 real sec
[M::mem_process_seqs] Processed 687372 reads in 89.471 CPU sec, 2.842 real sec
sorting block 14, #reads = 1080293 mem = 536882571 peak = 977436672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283720, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 182, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (172.13, 42.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 544)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 307, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (326.28, 162.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 278, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (288.47, 177.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683998 sequences (100000133 bp) in 2.029 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284199, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 287, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1387)
[M::mem_pestat] mean and std.dev: (282.50, 164.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1797)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 313, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (331.70, 164.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 282, 750)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1854)
[M::mem_pestat] mean and std.dev: (365.17, 293.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2406)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683618 sequences (100000092 bp) in 1.410 CPU sec, 1.409 real sec...
[M::mem_process_seqs] Processed 686188 reads in 142.883 CPU sec, 4.563 real sec
[M::mem_process_seqs] Processed 685326 reads in 89.072 CPU sec, 2.831 real sec
sorting block 15, #reads = 1082353 mem = 536878379 peak = 977436672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283131, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 367, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297)
[M::mem_pestat] mean and std.dev: (321.00, 184.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1671)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (337.26, 165.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 307, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (386.20, 209.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683268 sequences (100000055 bp) in 2.319 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283367, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 290, 1186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3086)
[M::mem_pestat] mean and std.dev: (565.45, 513.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4036)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 325, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (342.04, 168.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 574, 711)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665)
[M::mem_pestat] mean and std.dev: (464.58, 233.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2142)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683648 sequences (100000297 bp) in 1.397 CPU sec, 1.397 real sec...
[M::mem_process_seqs] Processed 683998 reads in 143.200 CPU sec, 4.567 real sec
[M::mem_process_seqs] Processed 683618 reads in 90.086 CPU sec, 2.864 real sec
sorting block 16, #reads = 1081243 mem = 536877237 peak = 977436672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282621, 5, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 242, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (342.90, 231.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (340.43, 166.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 318, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340)
[M::mem_pestat] mean and std.dev: (362.12, 252.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1732)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682414 sequences (100000229 bp) in 2.046 CPU sec, 2.175 real sec...
[M::process] read 682318 sequences (100000150 bp) in 1.380 CPU sec, 1.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282893, 8, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 232, 971)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449)
[M::mem_pestat] mean and std.dev: (446.17, 350.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3188)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (333.77, 162.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 223, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (259.15, 95.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 687)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683268 reads in 144.726 CPU sec, 4.624 real sec
sorting block 17, #reads = 1079078 mem = 536879703 peak = 977436672
[M::mem_process_seqs] Processed 683648 reads in 90.488 CPU sec, 2.875 real sec
sorting block 18, #reads = 1079084 mem = 536880397 peak = 991928320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282743, 2, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 214, 1076)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2954)
[M::mem_pestat] mean and std.dev: (585.27, 642.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3893)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (342.30, 165.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 500, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232)
[M::mem_pestat] mean and std.dev: (314.55, 168.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1580)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682592 sequences (100000073 bp) in 2.363 CPU sec, 2.504 real sec...
[M::process] read 683636 sequences (100000142 bp) in 1.420 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281978, 4, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 355, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (286.55, 147.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (342.95, 166.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 389, 728)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792)
[M::mem_pestat] mean and std.dev: (499.10, 431.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2324)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682414 reads in 147.478 CPU sec, 4.704 real sec
[M::mem_process_seqs] Processed 682318 reads in 91.350 CPU sec, 2.908 real sec
sorting block 19, #reads = 1078688 mem = 536888718 peak = 991928320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282218, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 171, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (217.85, 93.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (341.44, 166.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 332, 823)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1987)
[M::mem_pestat] mean and std.dev: (443.89, 352.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2569)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686886 sequences (100000114 bp) in 2.053 CPU sec, 2.191 real sec...
[M::process] read 686386 sequences (100000073 bp) in 1.377 CPU sec, 1.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282803, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 539, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412)
[M::mem_pestat] mean and std.dev: (381.90, 203.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1812)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (340.04, 168.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 305, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (288.19, 140.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682592 reads in 146.230 CPU sec, 4.673 real sec
[M::mem_process_seqs] Processed 683636 reads in 91.954 CPU sec, 2.924 real sec
sorting block 20, #reads = 1078180 mem = 536885570 peak = 991928320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283993, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 390, 1183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3129)
[M::mem_pestat] mean and std.dev: (701.92, 783.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4102)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 301, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (322.34, 161.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 328, 733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1737)
[M::mem_pestat] mean and std.dev: (449.11, 316.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2239)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685708 sequences (100000004 bp) in 2.374 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283497, 1, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 282, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (60, 560)
[M::mem_pestat] mean and std.dev: (252.73, 73.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 660)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 305, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (324.80, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 401, 995)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2585)
[M::mem_pestat] mean and std.dev: (480.52, 422.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3380)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684810 sequences (100000085 bp) in 1.360 CPU sec, 1.357 real sec...
[M::mem_process_seqs] Processed 686886 reads in 142.239 CPU sec, 4.535 real sec
[M::mem_process_seqs] Processed 686386 reads in 87.991 CPU sec, 2.797 real sec
sorting block 21, #reads = 1080420 mem = 536878681 peak = 991928320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283162, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (243, 311, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (307.14, 115.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 311, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (329.86, 164.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (312, 397, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (44, 714)
[M::mem_pestat] mean and std.dev: (336.58, 126.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684190 sequences (100000078 bp) in 2.015 CPU sec, 2.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282845, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 243, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576)
[M::mem_pestat] mean and std.dev: (260.27, 111.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (332.79, 165.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 405, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228)
[M::mem_pestat] mean and std.dev: (422.79, 268.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1575)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683648 sequences (100000023 bp) in 1.370 CPU sec, 1.362 real sec...
[M::mem_process_seqs] Processed 685708 reads in 139.255 CPU sec, 4.443 real sec
sorting block 22, #reads = 1083377 mem = 536879132 peak = 991928320
[M::mem_process_seqs] Processed 684810 reads in 87.697 CPU sec, 2.780 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 283273, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 327, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (260.11, 130.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (338.36, 167.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 293, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 537)
[M::mem_pestat] mean and std.dev: (286.90, 92.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 655)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 23, #reads = 1081590 mem = 536878882 peak = 998727680
[M::process] read 682878 sequences (100000146 bp) in 2.290 CPU sec, 2.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282814, 2, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 409, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1591)
[M::mem_pestat] mean and std.dev: (453.55, 335.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2062)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 325, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (341.16, 167.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (289, 307, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (427.24, 265.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1487)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684190 reads in 140.955 CPU sec, 4.491 real sec
[M::process] read 682338 sequences (100000242 bp) in 1.502 CPU sec, 1.513 real sec...
[M::mem_process_seqs] Processed 683648 reads in 89.237 CPU sec, 2.821 real sec
sorting block 24, #reads = 1081192 mem = 536879971 peak = 998727680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282517, 6, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (277, 373, 837)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957)
[M::mem_pestat] mean and std.dev: (413.32, 274.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2517)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (341.24, 165.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 254, 615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1533)
[M::mem_pestat] mean and std.dev: (387.69, 343.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1992)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681990 sequences (100000023 bp) in 2.095 CPU sec, 2.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282519, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 246, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (277.52, 121.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (342.77, 164.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681840 sequences (100000192 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_process_seqs] Processed 682878 reads in 141.365 CPU sec, 4.505 real sec
[M::mem_process_seqs] Processed 682338 reads in 89.089 CPU sec, 2.819 real sec
sorting block 25, #reads = 1079271 mem = 536879421 peak = 998727680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282220, 9, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 368, 682)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1574)
[M::mem_pestat] mean and std.dev: (397.08, 210.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2020)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (343.27, 163.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 228, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454)
[M::mem_pestat] mean and std.dev: (358.86, 248.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1879)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681708 sequences (100000083 bp) in 2.457 CPU sec, 2.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282353, 6, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 468, 954)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2440)
[M::mem_pestat] mean and std.dev: (469.83, 327.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3183)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (342.59, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (315, 381, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 717)
[M::mem_pestat] mean and std.dev: (352.69, 88.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690884 sequences (100000173 bp) in 1.420 CPU sec, 1.415 real sec...
[M::mem_process_seqs] Processed 681990 reads in 141.816 CPU sec, 4.505 real sec
sorting block 26, #reads = 1078494 mem = 536879762 peak = 998727680
[M::mem_process_seqs] Processed 681840 reads in 88.974 CPU sec, 2.813 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282153, 12, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 241, 1204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3204)
[M::mem_pestat] mean and std.dev: (467.33, 430.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4204)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (343.00, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1147, 1639, 6329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16693)
[M::mem_pestat] mean and std.dev: (2529.25, 2381.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21875)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 271, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (267.00, 102.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 27, #reads = 1077674 mem = 536877998 peak = 1000599552
[M::process] read 688520 sequences (100000221 bp) in 2.093 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 286004, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 203, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (249.57, 146.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 277, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (306.00, 158.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 226, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (212.45, 91.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686344 sequences (100000254 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 681708 reads in 145.384 CPU sec, 4.631 real sec
[M::mem_process_seqs] Processed 690884 reads in 93.989 CPU sec, 2.978 real sec
sorting block 28, #reads = 1077198 mem = 536878795 peak = 1000599552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285492, 12, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 239, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530)
[M::mem_pestat] mean and std.dev: (257.90, 126.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 764)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 293, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (317.37, 161.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 659, 1707)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4683)
[M::mem_pestat] mean and std.dev: (775.00, 648.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6171)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 237, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (337.95, 197.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1368)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685040 sequences (100000300 bp) in 2.345 CPU sec, 2.467 real sec...
[M::process] read 684302 sequences (100000236 bp) in 1.390 CPU sec, 1.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284935, 10, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 372, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (343.25, 183.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 310, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (329.40, 165.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 506, 1319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3693)
[M::mem_pestat] mean and std.dev: (887.10, 931.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4880)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 241, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (293.92, 105.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688520 reads in 144.365 CPU sec, 4.587 real sec
[M::mem_process_seqs] Processed 686344 reads in 89.999 CPU sec, 2.851 real sec
sorting block 29, #reads = 1080041 mem = 536878919 peak = 1000599552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283633, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 361, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338)
[M::mem_pestat] mean and std.dev: (370.38, 215.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1730)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 317, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (334.88, 166.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 361, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148)
[M::mem_pestat] mean and std.dev: (338.92, 164.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1461)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684188 sequences (100000157 bp) in 2.081 CPU sec, 2.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284250, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 314, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (334.35, 138.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 322, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (339.69, 168.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683408 sequences (100000241 bp) in 1.380 CPU sec, 1.382 real sec...
[M::mem_process_seqs] Processed 685040 reads in 144.417 CPU sec, 4.603 real sec
[M::mem_process_seqs] Processed 684302 reads in 91.709 CPU sec, 2.904 real sec
sorting block 30, #reads = 1079020 mem = 536882388 peak = 1000599552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283800, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (276, 358, 831)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1941)
[M::mem_pestat] mean and std.dev: (578.79, 521.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2665)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 321, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (338.28, 166.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 253, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (264.60, 128.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683076 sequences (100000036 bp) in 2.393 CPU sec, 2.514 real sec...
[M::process] read 683632 sequences (100000055 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282994, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 353, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (318.86, 150.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (339.57, 166.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 684188 reads in 149.436 CPU sec, 4.750 real sec
sorting block 31, #reads = 1078532 mem = 536876959 peak = 1000599552
[M::mem_process_seqs] Processed 683408 reads in 94.230 CPU sec, 2.992 real sec
sorting block 32, #reads = 1076084 mem = 536878247 peak = 1090633728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282822, 6, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (305, 420, 921)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153)
[M::mem_pestat] mean and std.dev: (558.28, 368.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2769)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (339.21, 165.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 246, 898)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2278)
[M::mem_pestat] mean and std.dev: (508.32, 384.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2968)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683026 sequences (100000286 bp) in 2.122 CPU sec, 2.250 real sec...
[M::process] read 688498 sequences (100000283 bp) in 1.440 CPU sec, 1.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283284, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 304, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (254.38, 77.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 319, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (336.27, 164.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 683076 reads in 151.035 CPU sec, 4.807 real sec
[M::mem_process_seqs] Processed 683632 reads in 95.533 CPU sec, 3.030 real sec
sorting block 33, #reads = 1075602 mem = 536878280 peak = 1090633728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283363, 9, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 374, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100)
[M::mem_pestat] mean and std.dev: (337.18, 142.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1404)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (339.99, 165.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 267, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 515)
[M::mem_pestat] mean and std.dev: (269.20, 85.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 611)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 691508 sequences (100000260 bp) in 2.149 CPU sec, 2.263 real sec...
[M::process] read 689468 sequences (100000125 bp) in 1.450 CPU sec, 1.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285252, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 210, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (209.00, 81.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 292, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (315.60, 159.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (294, 440, 574)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134)
[M::mem_pestat] mean and std.dev: (418.84, 179.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1414)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683026 reads in 153.057 CPU sec, 4.875 real sec
[M::mem_process_seqs] Processed 688498 reads in 99.324 CPU sec, 3.149 real sec
sorting block 34, #reads = 1075092 mem = 536880295 peak = 1090633728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 286799, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (259, 344, 1161)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2965)
[M::mem_pestat] mean and std.dev: (666.20, 672.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3867)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 274, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (299.97, 151.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687534 sequences (100000155 bp) in 2.118 CPU sec, 2.234 real sec...
[M::process] read 685836 sequences (100000140 bp) in 1.400 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 285816, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 217, 648)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712)
[M::mem_pestat] mean and std.dev: (409.50, 423.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2244)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 286, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (308.45, 153.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 349, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (349.17, 145.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 691508 reads in 157.725 CPU sec, 5.025 real sec
sorting block 35, #reads = 1075586 mem = 536879114 peak = 1090633728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284788, 0, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 276, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 523)
[M::mem_pestat] mean and std.dev: (259.55, 64.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 626)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 685326 sequences (100000174 bp) in 1.898 CPU sec, 1.902 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (188, 300, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (319.33, 158.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 242, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (241.84, 69.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687534 reads in 83.676 CPU sec, 2.649 real sec
[M::mem_process_seqs] Processed 689468 reads in 212.182 CPU sec, 6.741 real sec
sorting block 36, #reads = 1075574 mem = 536880102 peak = 1090633728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284501, 9, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 381, 586)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378)
[M::mem_pestat] mean and std.dev: (349.22, 170.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1774)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 307, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (325.71, 160.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 347, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (392.42, 185.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684150 sequences (100000032 bp) in 2.100 CPU sec, 2.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284334, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 300, 1056)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2936)
[M::mem_pestat] mean and std.dev: (534.58, 585.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3876)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 309, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (329.02, 163.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 243, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1222)
[M::mem_pestat] mean and std.dev: (319.55, 210.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1570)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683588 sequences (100000058 bp) in 1.360 CPU sec, 1.367 real sec...
[M::mem_process_seqs] Processed 685836 reads in 164.324 CPU sec, 5.223 real sec
[M::mem_process_seqs] Processed 685326 reads in 95.614 CPU sec, 3.030 real sec
sorting block 37, #reads = 1075545 mem = 536878493 peak = 1090633728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283594, 4, 24)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (335.24, 164.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (317, 469, 977)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2297)
[M::mem_pestat] mean and std.dev: (571.76, 452.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2957)
[M::mem_pestat] skip orientation RR
[M::process] read 683382 sequences (100000235 bp) in 2.384 CPU sec, 2.547 real sec...
[M::process] read 683112 sequences (100000029 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283304, 9, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 407, 1285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3471)
[M::mem_pestat] mean and std.dev: (814.48, 884.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4564)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (339.46, 167.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 263, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (298.17, 159.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684150 reads in 149.340 CPU sec, 4.744 real sec
sorting block 38, #reads = 1076365 mem = 536879317 peak = 1090633728
[M::mem_process_seqs] Processed 683588 reads in 93.801 CPU sec, 2.972 real sec
sorting block 39, #reads = 1075724 mem = 536881395 peak = 1120247808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283500, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 405, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (306.00, 130.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 323, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (342.26, 169.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 348, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212)
[M::mem_pestat] mean and std.dev: (370.38, 221.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1547)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688610 sequences (100000257 bp) in 2.046 CPU sec, 2.177 real sec...
[M::process] read 689376 sequences (100000197 bp) in 1.430 CPU sec, 1.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283248, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (301, 366, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (81, 631)
[M::mem_pestat] mean and std.dev: (360.80, 147.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 325, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (344.94, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 263, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (269.25, 156.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683382 reads in 150.035 CPU sec, 4.778 real sec
[M::mem_process_seqs] Processed 683112 reads in 95.633 CPU sec, 3.036 real sec
sorting block 40, #reads = 1075161 mem = 536879127 peak = 1120247808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284855, 6, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 229, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (271.94, 144.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 292, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (317.05, 162.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 285, 842)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2126)
[M::mem_pestat] mean and std.dev: (586.73, 603.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3001)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687262 sequences (100000037 bp) in 2.139 CPU sec, 2.284 real sec...
[M::process] read 686096 sequences (100000298 bp) in 1.380 CPU sec, 1.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 285618, 7, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 273, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110)
[M::mem_pestat] mean and std.dev: (399.38, 216.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1398)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 286, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (311.85, 159.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 220, 730)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862)
[M::mem_pestat] mean and std.dev: (357.08, 240.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2428)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688610 reads in 152.499 CPU sec, 4.856 real sec
[M::mem_process_seqs] Processed 689376 reads in 92.821 CPU sec, 2.946 real sec
sorting block 41, #reads = 1074531 mem = 536879742 peak = 1120247808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284875, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 227, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108)
[M::mem_pestat] mean and std.dev: (382.14, 320.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1662)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 298, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (321.22, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 365, 831)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1981)
[M::mem_pestat] mean and std.dev: (521.64, 430.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2556)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685508 sequences (100000293 bp) in 2.035 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284344, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1626)
[M::mem_pestat] mean and std.dev: (414.96, 329.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2105)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 685288 sequences (100000046 bp) in 1.380 CPU sec, 1.381 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (191, 306, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (326.24, 163.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 687262 reads in 142.416 CPU sec, 4.536 real sec
sorting block 42, #reads = 1080345 mem = 536878318 peak = 1120247808
[M::mem_process_seqs] Processed 686096 reads in 87.977 CPU sec, 2.792 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283619, 4, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 201, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (165.33, 45.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 466)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 307, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (326.16, 161.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 364, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148)
[M::mem_pestat] mean and std.dev: (305.05, 122.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1457)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 43, #reads = 1080263 mem = 536880179 peak = 1120247808
[M::process] read 685912 sequences (100000139 bp) in 2.372 CPU sec, 2.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283823, 3, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 115, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (231.00, 154.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1339)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 308, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (327.86, 162.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (337, 482, 2088)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5590)
[M::mem_pestat] mean and std.dev: (1225.79, 1383.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7341)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685398 sequences (100000124 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_process_seqs] Processed 685508 reads in 145.488 CPU sec, 4.624 real sec
[M::mem_process_seqs] Processed 685288 reads in 90.984 CPU sec, 2.883 real sec
sorting block 44, #reads = 1079239 mem = 536880362 peak = 1120247808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284266, 8, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 253, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340)
[M::mem_pestat] mean and std.dev: (341.65, 240.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1736)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 303, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (323.87, 161.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 241, 1149)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3043)
[M::mem_pestat] mean and std.dev: (551.50, 466.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3990)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684076 sequences (100000032 bp) in 2.104 CPU sec, 2.233 real sec...
[M::process] read 683914 sequences (100000203 bp) in 1.410 CPU sec, 1.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284119, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 319, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (256.94, 129.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 311, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (331.48, 165.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 313, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203)
[M::mem_pestat] mean and std.dev: (320.78, 186.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1535)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685912 reads in 147.844 CPU sec, 4.709 real sec
[M::mem_process_seqs] Processed 685398 reads in 93.286 CPU sec, 2.957 real sec
sorting block 45, #reads = 1078740 mem = 536878767 peak = 1120247808
[M::process] read 687116 sequences (100000112 bp) in 2.364 CPU sec, 2.486 real sec...
[M::process] read 693130 sequences (100000209 bp) in 1.440 CPU sec, 1.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282988, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 261, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279)
[M::mem_pestat] mean and std.dev: (343.38, 196.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1643)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 318, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (339.29, 169.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 311, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167)
[M::mem_pestat] mean and std.dev: (316.64, 252.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1509)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683914 reads in 85.498 CPU sec, 2.697 real sec
sorting block 46, #reads = 1076675 mem = 536885488 peak = 1120247808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283478, 3, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 255, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (299.55, 200.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (338.16, 168.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 237, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (254.32, 126.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284278, 7, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (252, 413, 880)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2136)
[M::mem_pestat] mean and std.dev: (487.31, 291.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2764)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 299, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (323.82, 165.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 356, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092)
[M::mem_pestat] mean and std.dev: (327.93, 133.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1385)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 691906 sequences (100000184 bp) in 1.670 CPU sec, 1.673 real sec...
[M::mem_process_seqs] Processed 684076 reads in 237.051 CPU sec, 7.529 real sec
[M::mem_process_seqs] Processed 687116 reads in 116.815 CPU sec, 3.735 real sec
sorting block 47, #reads = 1076059 mem = 536880268 peak = 1120247808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 287830, 3, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 246, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159)
[M::mem_pestat] mean and std.dev: (247.60, 121.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 267, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (293.90, 149.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 153, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (232.00, 139.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688708 sequences (100000056 bp) in 2.299 CPU sec, 2.455 real sec...
[M::process] read 687380 sequences (100000103 bp) in 1.377 CPU sec, 1.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286901, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 278, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (263.87, 94.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 272, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (297.06, 149.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 296, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1235)
[M::mem_pestat] mean and std.dev: (359.35, 293.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1584)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 693130 reads in 159.992 CPU sec, 5.095 real sec
[M::mem_process_seqs] Processed 691906 reads in 100.066 CPU sec, 3.182 real sec
sorting block 48, #reads = 1073535 mem = 536880714 peak = 1120247808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 285170, 7, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 306, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (287.05, 102.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 289, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (310.20, 153.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 286, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (283.21, 113.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686532 sequences (100000299 bp) in 2.401 CPU sec, 2.558 real sec...
[M::process] read 685596 sequences (100000116 bp) in 1.540 CPU sec, 1.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284395, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 302, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1535)
[M::mem_pestat] mean and std.dev: (413.58, 348.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1982)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 300, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (320.02, 159.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 286, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (361.45, 184.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1437)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688708 reads in 154.078 CPU sec, 4.892 real sec
sorting block 49, #reads = 1075756 mem = 536885723 peak = 1120247808
[M::mem_process_seqs] Processed 687380 reads in 95.465 CPU sec, 3.025 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284512, 4, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 263, 816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2078)
[M::mem_pestat] mean and std.dev: (535.95, 500.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2709)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 304, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (324.80, 161.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 359, 1585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4413)
[M::mem_pestat] mean and std.dev: (701.54, 742.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5827)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 50, #reads = 1076398 mem = 536877573 peak = 1120247808
[M::process] read 684552 sequences (100000020 bp) in 2.021 CPU sec, 2.140 real sec...
[M::process] read 683498 sequences (100000026 bp) in 1.390 CPU sec, 1.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283839, 7, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 299, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (272.75, 116.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 311, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (330.14, 163.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 272, 618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1500)
[M::mem_pestat] mean and std.dev: (363.39, 244.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1941)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686532 reads in 151.836 CPU sec, 4.844 real sec
[M::mem_process_seqs] Processed 685596 reads in 95.170 CPU sec, 3.024 real sec
sorting block 51, #reads = 1076347 mem = 536880489 peak = 1120247808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283896, 7, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 401, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (345.05, 144.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (333.07, 163.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (314, 447, 846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1910)
[M::mem_pestat] mean and std.dev: (597.67, 397.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2442)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682994 sequences (100000077 bp) in 2.242 CPU sec, 2.402 real sec...
[M::process] read 252704 sequences (36980940 bp) in 0.690 CPU sec, 0.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282928, 7, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 139, 769)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2077)
[M::mem_pestat] mean and std.dev: (498.47, 510.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2731)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 325, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (340.72, 166.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 349, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 629)
[M::mem_pestat] mean and std.dev: (353.32, 116.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684552 reads in 146.980 CPU sec, 4.701 real sec
[M::mem_process_seqs] Processed 683498 reads in 91.667 CPU sec, 2.936 real sec
sorting block 52, #reads = 1077074 mem = 536879941 peak = 1120247808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283238, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 340, 808)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942)
[M::mem_pestat] mean and std.dev: (457.61, 281.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2509)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (339.00, 165.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 199, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (228.35, 95.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 104757, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (338.11, 165.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682994 reads in 102.430 CPU sec, 3.335 real sec
[M::mem_process_seqs] Processed 252704 reads in 42.339 CPU sec, 1.374 real sec
sorting block 53, #reads = 1076517 mem = 536878528 peak = 1151389696
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 58469320
overall: 69639876608 mem 8215.640 user 272.577 sys 1301.768 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1301.807 sec; CPU: 8488.220 sec
sorting block 54, #reads = 551478 mem = 275356147 peak = 1151389696
uniq header count 1
merging 55 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3564544602
output reads: 58783014
bam_mem_sort: 55 calls 19.745 user 0.183 sys 20.026 real
bam_write: 55 calls 48.271 user 0.984 sys 49.511 real
execute: 1 calls 11.164 user 0.865 sys 1322.303 real
merge_files: 1 calls 11.142 user 0.845 sys 19.736 real
parse_chunk: 873 calls 26.408 user 7.725 sys 34.912 real
read_chunk: 2356 calls 21.944 user 21.106 sys 228.364 real
sort_block: 1 calls 17.005 user 20.616 sys 221.046 real
write_chunk: 1481 calls 10.796 user 0.679 sys 18.988 real
overall: 2730074112 mem 732.683 user 36.858 sys 1322.331 real
ls: cannot access '*.cram': No such file or directory