This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cVw-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2190.472 CPU sec, 95.922 real sec
[M::process] read 679290 sequences (100000007 bp) in 1.727 CPU sec, 1.728 real sec...
[M::process] read 676656 sequences (100000266 bp) in 2.332 CPU sec, 3.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283564, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (343.62, 161.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676092 sequences (100000107 bp) in 2.362 CPU sec, 3.516 real sec...
[M::process] read 676538 sequences (100000004 bp) in 2.278 CPU sec, 3.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282417, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 350, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (363.38, 165.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679290 reads in 286.916 CPU sec, 11.745 real sec
[M::mem_process_seqs] Processed 676656 reads in 159.723 CPU sec, 6.641 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281769, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 351, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (365.11, 165.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676758 sequences (100000284 bp) in 3.229 CPU sec, 4.010 real sec...
sorting block 0, #reads = 1083316 mem = 536880055 peak = 553844736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282302, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (366.50, 167.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678326 sequences (100000165 bp) in 2.434 CPU sec, 2.495 real sec...
[M::mem_process_seqs] Processed 676092 reads in 234.357 CPU sec, 9.545 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282533, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 350, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (363.50, 165.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678228 sequences (100000010 bp) in 2.471 CPU sec, 2.542 real sec...
[M::mem_process_seqs] Processed 676758 reads in 122.778 CPU sec, 4.531 real sec
sorting block 1, #reads = 1079720 mem = 536876871 peak = 676663296
[M::mem_process_seqs] Processed 676538 reads in 380.293 CPU sec, 15.045 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283321, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (352.99, 164.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282901, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (352.81, 164.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678508 sequences (100000108 bp) in 3.360 CPU sec, 4.023 real sec...
sorting block 2, #reads = 1081917 mem = 536879821 peak = 801939456
[M::mem_process_seqs] Processed 678326 reads in 262.474 CPU sec, 10.066 real sec
[M::mem_process_seqs] Processed 678228 reads in 160.020 CPU sec, 6.156 real sec
[M::process] read 678798 sequences (100000025 bp) in 3.215 CPU sec, 3.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282663, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (351.49, 163.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678508 reads in 137.024 CPU sec, 5.457 real sec
sorting block 3, #reads = 1082692 mem = 536878196 peak = 801939456
[M::process] read 678664 sequences (100000106 bp) in 3.249 CPU sec, 3.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282487, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (348.43, 162.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678798 reads in 136.339 CPU sec, 5.301 real sec
[M::process] read 676248 sequences (100000010 bp) in 3.052 CPU sec, 3.609 real sec...
sorting block 4, #reads = 1082805 mem = 536882954 peak = 801939456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283209, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (350.05, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675684 sequences (100000236 bp) in 2.978 CPU sec, 3.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282466, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (366.17, 166.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678664 reads in 245.442 CPU sec, 9.124 real sec
[M::mem_process_seqs] Processed 676248 reads in 160.807 CPU sec, 6.190 real sec
sorting block 5, #reads = 1082799 mem = 536880853 peak = 801939456
[M::process] read 677070 sequences (100000168 bp) in 3.679 CPU sec, 4.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282102, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 359, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (371.68, 167.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675684 reads in 131.534 CPU sec, 5.127 real sec
[M::process] read 678388 sequences (100000063 bp) in 3.451 CPU sec, 4.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282168, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 347, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (362.60, 167.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677070 reads in 138.502 CPU sec, 5.475 real sec
sorting block 6, #reads = 1082172 mem = 536878216 peak = 801939456
[M::process] read 678040 sequences (100000283 bp) in 3.381 CPU sec, 4.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282600, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (352.06, 164.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678388 reads in 138.420 CPU sec, 5.374 real sec
[M::process] read 677668 sequences (100000176 bp) in 3.516 CPU sec, 3.975 real sec...
sorting block 7, #reads = 1082711 mem = 536878733 peak = 801939456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282717, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 339, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (355.59, 165.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678040 reads in 142.795 CPU sec, 5.221 real sec
[M::process] read 677488 sequences (100000108 bp) in 3.465 CPU sec, 3.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282538, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (357.34, 165.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677668 reads in 136.396 CPU sec, 4.765 real sec
sorting block 8, #reads = 1082965 mem = 536876782 peak = 801939456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282303, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (356.17, 164.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677572 sequences (100000219 bp) in 4.085 CPU sec, 4.678 real sec...
[M::mem_process_seqs] Processed 677488 reads in 135.049 CPU sec, 4.588 real sec
sorting block 9, #reads = 1083111 mem = 536878679 peak = 801939456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282603, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (354.02, 164.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677628 sequences (100000102 bp) in 3.524 CPU sec, 3.858 real sec...
[M::mem_process_seqs] Processed 677572 reads in 125.637 CPU sec, 4.210 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282322, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (352.57, 163.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677628 reads in 119.250 CPU sec, 3.995 real sec
[M::process] read 676042 sequences (100000004 bp) in 3.971 CPU sec, 4.685 real sec...
sorting block 10, #reads = 1083062 mem = 536878914 peak = 801939456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281859, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 346, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (361.85, 164.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675704 sequences (100000237 bp) in 3.389 CPU sec, 3.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282086, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 348, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (363.09, 164.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677466 sequences (100000030 bp) in 3.164 CPU sec, 3.439 real sec...
[M::mem_process_seqs] Processed 675704 reads in 116.660 CPU sec, 3.762 real sec
sorting block 11, #reads = 1082780 mem = 536882677 peak = 801939456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282390, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (351.25, 162.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677466 reads in 120.820 CPU sec, 3.922 real sec
[M::process] read 677892 sequences (100000247 bp) in 4.232 CPU sec, 4.649 real sec...
[M::mem_process_seqs] Processed 676042 reads in 356.766 CPU sec, 11.987 real sec
sorting block 12, #reads = 1082575 mem = 536879350 peak = 808288256
[M::process] read 676914 sequences (100000089 bp) in 2.862 CPU sec, 3.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282490, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.78, 161.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282071, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (357.41, 164.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677892 reads in 211.008 CPU sec, 6.868 real sec
[M::mem_process_seqs] Processed 676914 reads in 137.945 CPU sec, 4.432 real sec
[M::process] read 675712 sequences (100000140 bp) in 3.975 CPU sec, 4.443 real sec...
sorting block 13, #reads = 1082541 mem = 536879874 peak = 816783360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281207, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (362.26, 163.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675712 reads in 120.439 CPU sec, 4.035 real sec
[M::process] read 677240 sequences (100000184 bp) in 3.747 CPU sec, 4.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281879, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (351.04, 161.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1081665 mem = 536879403 peak = 816783360
[M::mem_process_seqs] Processed 677240 reads in 112.381 CPU sec, 3.765 real sec
[M::process] read 677064 sequences (100000013 bp) in 4.172 CPU sec, 4.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282182, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (352.33, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677064 reads in 115.906 CPU sec, 3.849 real sec
[M::process] read 677346 sequences (100000001 bp) in 3.654 CPU sec, 4.087 real sec...
sorting block 15, #reads = 1080591 mem = 536882279 peak = 816783360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281975, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (348.60, 159.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677346 reads in 115.754 CPU sec, 3.843 real sec
[M::process] read 677058 sequences (100000096 bp) in 3.595 CPU sec, 4.087 real sec...
sorting block 16, #reads = 1082842 mem = 536878131 peak = 830607360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282092, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.61, 161.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675472 sequences (100000206 bp) in 2.794 CPU sec, 3.246 real sec...
[M::mem_process_seqs] Processed 677058 reads in 109.939 CPU sec, 3.618 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281494, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (362.71, 163.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1081967 mem = 536879369 peak = 895717376
[M::mem_process_seqs] Processed 675472 reads in 106.177 CPU sec, 3.458 real sec
[M::process] read 675816 sequences (100000300 bp) in 3.958 CPU sec, 4.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281960, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (361.30, 162.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677188 sequences (100000247 bp) in 2.573 CPU sec, 2.882 real sec...
[M::mem_process_seqs] Processed 675816 reads in 100.831 CPU sec, 3.289 real sec
sorting block 18, #reads = 1081464 mem = 536880094 peak = 895717376
[M::process] read 677360 sequences (100000215 bp) in 3.046 CPU sec, 3.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281818, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (348.49, 160.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677348 sequences (100000008 bp) in 2.108 CPU sec, 2.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282120, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (349.73, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677188 reads in 199.740 CPU sec, 6.293 real sec
[M::mem_process_seqs] Processed 677360 reads in 130.281 CPU sec, 4.111 real sec
[M::process] read 676368 sequences (100000224 bp) in 2.666 CPU sec, 2.830 real sec...
sorting block 19, #reads = 1082018 mem = 536881532 peak = 895717376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282634, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (350.11, 161.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281948, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (355.23, 162.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676378 sequences (100000163 bp) in 3.819 CPU sec, 4.208 real sec...
[M::mem_process_seqs] Processed 677348 reads in 194.748 CPU sec, 6.251 real sec
[M::mem_process_seqs] Processed 676368 reads in 132.324 CPU sec, 4.214 real sec
sorting block 20, #reads = 1082492 mem = 536878779 peak = 895717376
[M::process] read 677190 sequences (100000086 bp) in 3.477 CPU sec, 3.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282544, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (354.71, 161.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281925, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (353.69, 164.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677042 sequences (100000224 bp) in 3.335 CPU sec, 3.795 real sec...
[M::mem_process_seqs] Processed 676378 reads in 218.302 CPU sec, 7.048 real sec
sorting block 21, #reads = 1082471 mem = 536880641 peak = 943939584
[M::mem_process_seqs] Processed 677190 reads in 150.246 CPU sec, 4.827 real sec
[M::process] read 676838 sequences (100000145 bp) in 4.019 CPU sec, 4.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282248, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (356.58, 164.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677042 reads in 120.787 CPU sec, 4.023 real sec
sorting block 22, #reads = 1083322 mem = 536877643 peak = 943939584
[M::process] read 676522 sequences (100000152 bp) in 2.718 CPU sec, 2.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282045, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (357.50, 164.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675492 sequences (100000130 bp) in 3.316 CPU sec, 3.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282216, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 343, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (360.14, 166.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676838 reads in 214.013 CPU sec, 6.845 real sec
[M::mem_process_seqs] Processed 676522 reads in 146.779 CPU sec, 4.668 real sec
sorting block 23, #reads = 1082319 mem = 536877218 peak = 943939584
[M::process] read 678944 sequences (100000176 bp) in 3.449 CPU sec, 3.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282046, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 358, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (371.24, 168.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1082193 mem = 536878701 peak = 943939584
[M::process] read 678150 sequences (100000270 bp) in 2.834 CPU sec, 3.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283762, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (346.25, 161.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675492 reads in 210.479 CPU sec, 6.770 real sec
[M::mem_process_seqs] Processed 678944 reads in 142.057 CPU sec, 4.521 real sec
[M::process] read 677808 sequences (100000183 bp) in 2.872 CPU sec, 3.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282165, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (351.43, 163.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1082368 mem = 536880725 peak = 943939584
[M::process] read 676638 sequences (100000022 bp) in 3.339 CPU sec, 3.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282577, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (350.32, 161.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678150 reads in 200.767 CPU sec, 6.448 real sec
[M::mem_process_seqs] Processed 677808 reads in 139.096 CPU sec, 4.438 real sec
[M::process] read 677460 sequences (100000106 bp) in 2.608 CPU sec, 2.742 real sec...
sorting block 26, #reads = 1084500 mem = 536879003 peak = 943939584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282036, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (364.22, 166.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677796 sequences (100000157 bp) in 3.195 CPU sec, 3.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281992, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 343, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (357.54, 164.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676638 reads in 201.789 CPU sec, 6.459 real sec
[M::mem_process_seqs] Processed 677460 reads in 140.742 CPU sec, 4.470 real sec
sorting block 27, #reads = 1084517 mem = 536879096 peak = 943939584
[M::process] read 677482 sequences (100000015 bp) in 2.860 CPU sec, 2.969 real sec...
[M::process] read 677242 sequences (100000267 bp) in 3.017 CPU sec, 3.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282508, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 345, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (360.92, 167.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677482 reads in 113.161 CPU sec, 3.585 real sec
sorting block 28, #reads = 1083287 mem = 536877878 peak = 951169024
[M::process] read 677368 sequences (100000095 bp) in 3.408 CPU sec, 3.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282099, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 345, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (360.25, 167.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283056, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (356.78, 166.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677242 reads in 138.048 CPU sec, 4.462 real sec
[M::mem_process_seqs] Processed 677796 reads in 334.969 CPU sec, 10.809 real sec
sorting block 29, #reads = 1084245 mem = 536880114 peak = 952877056
[M::process] read 677722 sequences (100000092 bp) in 3.410 CPU sec, 3.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282344, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 343, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (358.09, 164.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677368 reads in 106.991 CPU sec, 3.592 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282545, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (353.79, 163.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677500 sequences (100000238 bp) in 3.559 CPU sec, 3.833 real sec...
[M::mem_process_seqs] Processed 677722 reads in 112.996 CPU sec, 3.787 real sec
sorting block 30, #reads = 1084468 mem = 536879507 peak = 952877056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282665, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (357.82, 166.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676078 sequences (100000190 bp) in 3.176 CPU sec, 3.359 real sec...
[M::mem_process_seqs] Processed 677500 reads in 110.951 CPU sec, 3.707 real sec
[M::process] read 676732 sequences (100000094 bp) in 3.038 CPU sec, 3.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281798, 15, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (366.88, 165.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3628, 3897, 3897)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3090, 4435)
[M::mem_pestat] mean and std.dev: (3852.17, 100.25)
[M::mem_pestat] low and high boundaries for proper pairs: (2821, 4704)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 31, #reads = 1085107 mem = 536879045 peak = 952877056
[M::mem_process_seqs] Processed 676078 reads in 113.492 CPU sec, 3.791 real sec
[M::process] read 677832 sequences (100000193 bp) in 2.905 CPU sec, 3.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281959, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (361.83, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 32, #reads = 1083249 mem = 536878932 peak = 952877056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282773, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (354.88, 164.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677546 sequences (100000153 bp) in 3.047 CPU sec, 3.234 real sec...
[M::mem_process_seqs] Processed 676732 reads in 197.892 CPU sec, 6.362 real sec
[M::mem_process_seqs] Processed 677832 reads in 133.775 CPU sec, 4.256 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282896, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (356.75, 165.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677456 sequences (100000141 bp) in 3.809 CPU sec, 4.133 real sec...
[M::mem_process_seqs] Processed 677546 reads in 106.839 CPU sec, 3.565 real sec
sorting block 33, #reads = 1084535 mem = 536878030 peak = 952877056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282455, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (356.58, 164.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677456 reads in 110.862 CPU sec, 3.717 real sec
[M::process] read 677480 sequences (100000239 bp) in 3.599 CPU sec, 3.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283297, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 34, #reads = 1084190 mem = 536879038 peak = 952877056
[M::mem_pestat] (25, 50, 75) percentile: (225, 340, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (355.85, 164.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677480 reads in 108.461 CPU sec, 3.622 real sec
[M::process] read 677072 sequences (100000178 bp) in 3.659 CPU sec, 3.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282548, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (357.19, 164.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677072 sequences (100000256 bp) in 3.293 CPU sec, 3.592 real sec...
[M::mem_process_seqs] Processed 677072 reads in 111.088 CPU sec, 3.709 real sec
sorting block 35, #reads = 1083593 mem = 536877860 peak = 952877056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282772, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 343, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (359.11, 166.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677072 reads in 115.850 CPU sec, 3.859 real sec
[M::process] read 676346 sequences (100000083 bp) in 3.708 CPU sec, 4.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282631, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (359.63, 164.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1083458 mem = 536879406 peak = 952877056
[M::process] read 675098 sequences (100000301 bp) in 3.050 CPU sec, 3.245 real sec...
[M::mem_process_seqs] Processed 676346 reads in 106.867 CPU sec, 3.569 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281844, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 355, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (369.84, 166.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675098 reads in 114.908 CPU sec, 3.833 real sec
[M::process] read 675718 sequences (100000217 bp) in 3.965 CPU sec, 4.374 real sec...
sorting block 37, #reads = 1083361 mem = 536878028 peak = 952877056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281937, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (365.57, 165.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677144 sequences (100000042 bp) in 3.233 CPU sec, 3.565 real sec...
[M::mem_process_seqs] Processed 675718 reads in 112.990 CPU sec, 3.831 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282577, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.45, 162.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677144 reads in 118.960 CPU sec, 4.016 real sec
[M::process] read 677282 sequences (100000123 bp) in 3.883 CPU sec, 4.393 real sec...
sorting block 38, #reads = 1082493 mem = 536880001 peak = 952877056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282962, 11, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (351.77, 162.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 47, 816)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2354)
[M::mem_pestat] mean and std.dev: (419.09, 569.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3123)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677282 reads in 112.925 CPU sec, 3.844 real sec
[M::process] read 676440 sequences (100000041 bp) in 3.456 CPU sec, 4.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282074, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 344, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (360.46, 166.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1082931 mem = 536880673 peak = 952877056
[M::mem_process_seqs] Processed 676440 reads in 113.075 CPU sec, 3.816 real sec
[M::process] read 677326 sequences (100000208 bp) in 3.708 CPU sec, 4.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282590, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (355.39, 166.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677274 sequences (100000108 bp) in 3.448 CPU sec, 3.902 real sec...
[M::mem_process_seqs] Processed 677326 reads in 115.541 CPU sec, 3.958 real sec
sorting block 40, #reads = 1083775 mem = 536880349 peak = 952877056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282713, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (355.31, 166.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677274 reads in 118.093 CPU sec, 3.988 real sec
[M::process] read 677132 sequences (100000170 bp) in 3.916 CPU sec, 4.271 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282374, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (355.10, 165.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677132 reads in 113.160 CPU sec, 3.823 real sec
[M::process] read 677492 sequences (100000251 bp) in 3.706 CPU sec, 4.064 real sec...
sorting block 41, #reads = 1083862 mem = 536878426 peak = 952877056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282358, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (348.19, 161.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677492 reads in 116.121 CPU sec, 3.929 real sec
[M::process] read 676408 sequences (100000160 bp) in 3.850 CPU sec, 4.197 real sec...
sorting block 42, #reads = 1083171 mem = 536878747 peak = 974950400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281919, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 342, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (358.54, 164.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676408 reads in 113.531 CPU sec, 3.869 real sec
[M::process] read 675432 sequences (100000054 bp) in 3.639 CPU sec, 4.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281954, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 349, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (363.96, 165.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675432 reads in 110.718 CPU sec, 3.721 real sec
[M::process] read 676886 sequences (100000006 bp) in 3.868 CPU sec, 4.230 real sec...
sorting block 43, #reads = 1082985 mem = 536880184 peak = 974950400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282768, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (354.06, 163.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676886 reads in 117.147 CPU sec, 3.963 real sec
[M::process] read 677248 sequences (100000249 bp) in 3.669 CPU sec, 4.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282024, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (350.59, 162.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1083494 mem = 536880369 peak = 974950400
[M::mem_process_seqs] Processed 677248 reads in 113.398 CPU sec, 3.813 real sec
[M::process] read 677306 sequences (100000178 bp) in 3.742 CPU sec, 4.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282951, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (352.34, 163.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677306 reads in 117.598 CPU sec, 3.949 real sec
[M::process] read 677282 sequences (100000109 bp) in 3.913 CPU sec, 4.287 real sec...
sorting block 45, #reads = 1082063 mem = 536878101 peak = 974950400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282362, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.67, 163.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677120 sequences (100000058 bp) in 3.566 CPU sec, 3.841 real sec...
[M::mem_process_seqs] Processed 677282 reads in 114.163 CPU sec, 3.866 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282628, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (352.02, 163.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677120 reads in 112.759 CPU sec, 3.791 real sec
sorting block 46, #reads = 1083652 mem = 536877484 peak = 974950400
[M::process] read 677060 sequences (100000145 bp) in 3.901 CPU sec, 4.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282960, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (353.14, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677472 sequences (100000073 bp) in 3.301 CPU sec, 3.577 real sec...
[M::mem_process_seqs] Processed 677060 reads in 113.718 CPU sec, 3.837 real sec
sorting block 47, #reads = 1082490 mem = 536876651 peak = 992686080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282110, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (354.40, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677472 reads in 117.478 CPU sec, 3.941 real sec
[M::process] read 677374 sequences (100000074 bp) in 4.098 CPU sec, 4.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282854, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (356.73, 167.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677374 reads in 113.683 CPU sec, 3.811 real sec
[M::process] read 677436 sequences (100000244 bp) in 3.771 CPU sec, 4.155 real sec...
sorting block 48, #reads = 1082890 mem = 536880503 peak = 992686080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282760, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (353.40, 165.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677436 reads in 119.292 CPU sec, 4.026 real sec
[M::process] read 676868 sequences (100000185 bp) in 3.802 CPU sec, 4.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282890, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 49, #reads = 1083212 mem = 536883657 peak = 995532800
[M::mem_pestat] (25, 50, 75) percentile: (227, 342, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (360.70, 169.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676868 reads in 114.967 CPU sec, 3.876 real sec
[M::process] read 679426 sequences (100000200 bp) in 3.729 CPU sec, 4.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283168, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 326, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (341.05, 157.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679426 reads in 126.281 CPU sec, 4.236 real sec
[M::process] read 678514 sequences (100000008 bp) in 4.102 CPU sec, 4.549 real sec...
sorting block 50, #reads = 1082832 mem = 536878876 peak = 995532800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283409, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 330, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (343.87, 156.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678514 reads in 118.221 CPU sec, 4.002 real sec
[M::process] read 678494 sequences (100000276 bp) in 3.670 CPU sec, 4.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282631, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (345.55, 157.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1081870 mem = 536877164 peak = 995532800
[M::mem_process_seqs] Processed 678494 reads in 119.926 CPU sec, 4.030 real sec
[M::process] read 678448 sequences (100000291 bp) in 3.873 CPU sec, 4.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283078, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (345.38, 157.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678448 reads in 116.763 CPU sec, 3.936 real sec
[M::process] read 678258 sequences (100000017 bp) in 3.684 CPU sec, 4.042 real sec...
sorting block 52, #reads = 1082139 mem = 536878247 peak = 995532800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283212, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (346.94, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678258 reads in 115.496 CPU sec, 3.892 real sec
[M::process] read 677816 sequences (100000070 bp) in 3.638 CPU sec, 3.990 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283344, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.40, 156.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677816 reads in 118.933 CPU sec, 4.020 real sec
[M::process] read 677288 sequences (100000215 bp) in 3.947 CPU sec, 4.296 real sec...
sorting block 53, #reads = 1082177 mem = 536879069 peak = 995532800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282323, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 338, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (352.19, 159.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677288 reads in 116.061 CPU sec, 3.918 real sec
[M::process] read 677362 sequences (100000135 bp) in 3.791 CPU sec, 4.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283243, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (357.39, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1083062 mem = 536879569 peak = 995532800
[M::mem_process_seqs] Processed 677362 reads in 119.955 CPU sec, 4.014 real sec
[M::process] read 675428 sequences (100000025 bp) in 3.848 CPU sec, 4.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281987, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 362, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (372.00, 164.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675428 reads in 116.282 CPU sec, 3.962 real sec
[M::process] read 675668 sequences (100000279 bp) in 3.818 CPU sec, 4.364 real sec...
sorting block 55, #reads = 1082173 mem = 536882103 peak = 995532800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282255, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 356, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (367.01, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675668 reads in 119.378 CPU sec, 4.068 real sec
[M::process] read 677828 sequences (100000128 bp) in 3.766 CPU sec, 4.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282910, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (352.48, 161.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677828 reads in 116.552 CPU sec, 3.995 real sec
[M::process] read 677880 sequences (100000170 bp) in 3.742 CPU sec, 4.151 real sec...
sorting block 56, #reads = 1081771 mem = 536877609 peak = 995532800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282815, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (350.10, 160.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677880 reads in 119.974 CPU sec, 4.063 real sec
[M::process] read 678016 sequences (100000045 bp) in 3.816 CPU sec, 4.220 real sec...
sorting block 57, #reads = 1081089 mem = 536879596 peak = 995532800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282689, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (352.98, 162.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678016 reads in 116.212 CPU sec, 3.928 real sec
[M::process] read 677674 sequences (100000109 bp) in 3.839 CPU sec, 4.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282974, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 338, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (353.00, 162.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677674 reads in 118.374 CPU sec, 4.010 real sec
[M::process] read 677624 sequences (100000049 bp) in 3.779 CPU sec, 4.146 real sec...
sorting block 58, #reads = 1082742 mem = 536877583 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281815, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (352.00, 160.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677624 reads in 116.920 CPU sec, 3.941 real sec
[M::process] read 677376 sequences (100000151 bp) in 3.743 CPU sec, 4.136 real sec...
sorting block 59, #reads = 1084722 mem = 536879172 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282640, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (351.71, 160.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677376 reads in 118.691 CPU sec, 4.006 real sec
[M::process] read 677552 sequences (100000226 bp) in 3.831 CPU sec, 4.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282651, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (352.66, 161.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677552 reads in 117.896 CPU sec, 4.011 real sec
[M::process] read 677468 sequences (100000172 bp) in 3.705 CPU sec, 4.073 real sec...
sorting block 60, #reads = 1083057 mem = 536880970 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282766, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (355.05, 162.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677468 reads in 118.602 CPU sec, 4.047 real sec
[M::process] read 677130 sequences (100000182 bp) in 3.754 CPU sec, 4.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282593, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (356.88, 164.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1084274 mem = 536879750 peak = 1008558080
[M::mem_process_seqs] Processed 677130 reads in 121.160 CPU sec, 4.081 real sec
[M::process] read 676954 sequences (100000259 bp) in 3.950 CPU sec, 4.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282251, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (357.27, 162.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675416 sequences (100000246 bp) in 3.437 CPU sec, 3.828 real sec...
[M::mem_process_seqs] Processed 676954 reads in 116.582 CPU sec, 3.979 real sec
sorting block 62, #reads = 1084195 mem = 536879038 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281732, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (366.38, 162.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675416 reads in 116.655 CPU sec, 3.961 real sec
[M::process] read 675410 sequences (100000108 bp) in 3.627 CPU sec, 3.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282528, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 354, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (366.28, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675410 reads in 113.069 CPU sec, 3.824 real sec
[M::process] read 677208 sequences (100000059 bp) in 3.694 CPU sec, 4.009 real sec...
sorting block 63, #reads = 1083822 mem = 536878837 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282364, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (353.60, 161.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677208 reads in 120.064 CPU sec, 4.089 real sec
[M::process] read 676998 sequences (100000181 bp) in 3.697 CPU sec, 4.326 real sec...
sorting block 64, #reads = 1083430 mem = 536879389 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282091, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (354.00, 161.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676820 sequences (100000073 bp) in 3.531 CPU sec, 4.013 real sec...
[M::mem_process_seqs] Processed 676998 reads in 117.617 CPU sec, 4.044 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282320, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 339, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (355.73, 163.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676820 reads in 119.915 CPU sec, 4.053 real sec
[M::process] read 677154 sequences (100000163 bp) in 4.136 CPU sec, 4.538 real sec...
sorting block 65, #reads = 1082899 mem = 536878897 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282614, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (354.40, 163.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677164 sequences (100000197 bp) in 3.490 CPU sec, 3.848 real sec...
[M::mem_process_seqs] Processed 677154 reads in 117.569 CPU sec, 4.070 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282362, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (353.39, 162.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 66, #reads = 1083172 mem = 536878865 peak = 1008558080
[M::mem_process_seqs] Processed 677164 reads in 119.961 CPU sec, 4.101 real sec
[M::process] read 677044 sequences (100000287 bp) in 3.818 CPU sec, 4.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282397, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (355.08, 164.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677044 reads in 122.903 CPU sec, 4.171 real sec
[M::process] read 677266 sequences (100000098 bp) in 3.777 CPU sec, 4.172 real sec...
sorting block 67, #reads = 1083561 mem = 536883183 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282047, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (351.25, 162.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677266 reads in 120.567 CPU sec, 4.111 real sec
[M::process] read 677512 sequences (100000239 bp) in 3.632 CPU sec, 4.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282113, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (347.25, 159.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677512 reads in 117.706 CPU sec, 3.979 real sec
[M::process] read 677320 sequences (100000096 bp) in 3.753 CPU sec, 4.080 real sec...
sorting block 68, #reads = 1082796 mem = 536880270 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281677, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (352.66, 163.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677320 reads in 119.232 CPU sec, 4.083 real sec
[M::process] read 677472 sequences (100000197 bp) in 3.695 CPU sec, 4.372 real sec...
sorting block 69, #reads = 1082378 mem = 536880167 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282091, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (349.66, 162.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677644 sequences (100000245 bp) in 3.536 CPU sec, 3.855 real sec...
[M::mem_process_seqs] Processed 677472 reads in 115.304 CPU sec, 3.883 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282628, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (349.48, 162.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677644 reads in 113.011 CPU sec, 3.785 real sec
[M::process] read 677288 sequences (100000083 bp) in 3.780 CPU sec, 4.053 real sec...
sorting block 70, #reads = 1083606 mem = 536879310 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282412, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (351.28, 162.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677288 reads in 113.568 CPU sec, 3.812 real sec
[M::process] read 677424 sequences (100000130 bp) in 3.761 CPU sec, 4.047 real sec...
sorting block 71, #reads = 1084099 mem = 536880648 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281962, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (350.81, 162.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677424 reads in 116.175 CPU sec, 3.916 real sec
[M::process] read 677248 sequences (100000173 bp) in 4.228 CPU sec, 4.703 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282393, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (351.44, 162.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677248 reads in 121.982 CPU sec, 4.133 real sec
[M::process] read 677344 sequences (100000024 bp) in 3.813 CPU sec, 4.140 real sec...
sorting block 72, #reads = 1082599 mem = 536878751 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282462, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (353.34, 164.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677344 reads in 119.327 CPU sec, 4.014 real sec
[M::process] read 677268 sequences (100000175 bp) in 4.069 CPU sec, 4.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282912, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (352.69, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677268 reads in 116.036 CPU sec, 3.915 real sec
sorting block 73, #reads = 1083692 mem = 536879307 peak = 1008558080
[M::process] read 677102 sequences (100000143 bp) in 4.755 CPU sec, 5.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282649, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (355.63, 166.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677102 reads in 117.936 CPU sec, 4.003 real sec
[M::process] read 677652 sequences (100000021 bp) in 3.708 CPU sec, 4.067 real sec...
sorting block 74, #reads = 1083546 mem = 536878806 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282446, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (352.06, 166.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677652 reads in 114.066 CPU sec, 3.808 real sec
[M::process] read 677540 sequences (100000132 bp) in 3.853 CPU sec, 4.186 real sec...
sorting block 75, #reads = 1082865 mem = 536879393 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282261, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (354.84, 167.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677540 reads in 115.132 CPU sec, 3.823 real sec
[M::process] read 677972 sequences (100000292 bp) in 3.497 CPU sec, 3.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281939, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (346.82, 157.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677972 reads in 109.851 CPU sec, 3.653 real sec
[M::process] read 676926 sequences (100000246 bp) in 3.345 CPU sec, 3.820 real sec...
sorting block 76, #reads = 1082226 mem = 536879710 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281188, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 336, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (348.97, 156.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676926 reads in 97.986 CPU sec, 3.259 real sec
[M::process] read 676512 sequences (100000240 bp) in 3.051 CPU sec, 3.313 real sec...
sorting block 77, #reads = 1081837 mem = 536877512 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282319, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 343, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (355.79, 159.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676512 reads in 102.366 CPU sec, 3.401 real sec
[M::process] read 676438 sequences (100000055 bp) in 3.364 CPU sec, 3.743 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281908, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (353.44, 158.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676438 reads in 105.887 CPU sec, 3.486 real sec
sorting block 78, #reads = 1082801 mem = 536879428 peak = 1008558080
[M::process] read 676654 sequences (100000228 bp) in 3.499 CPU sec, 3.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281978, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 342, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (355.14, 159.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676978 sequences (100000231 bp) in 2.993 CPU sec, 3.343 real sec...
[M::mem_process_seqs] Processed 676654 reads in 108.828 CPU sec, 3.558 real sec
sorting block 79, #reads = 1082828 mem = 536879271 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281508, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (350.68, 158.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677478 sequences (100000208 bp) in 3.010 CPU sec, 3.191 real sec...
[M::mem_process_seqs] Processed 676978 reads in 105.170 CPU sec, 3.451 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282281, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (347.48, 156.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677478 reads in 109.345 CPU sec, 3.574 real sec
[M::process] read 677316 sequences (100000178 bp) in 3.787 CPU sec, 4.220 real sec...
sorting block 80, #reads = 1082870 mem = 536881283 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281984, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (348.99, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677316 reads in 102.148 CPU sec, 3.380 real sec
[M::process] read 676324 sequences (100000180 bp) in 3.154 CPU sec, 3.543 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282622, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (360.82, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676324 reads in 104.543 CPU sec, 3.458 real sec
sorting block 81, #reads = 1083440 mem = 536878059 peak = 1008558080
[M::process] read 675086 sequences (100000096 bp) in 3.359 CPU sec, 3.720 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281416, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 357, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (368.50, 162.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675412 sequences (100000264 bp) in 3.138 CPU sec, 3.455 real sec...
[M::mem_process_seqs] Processed 675086 reads in 109.014 CPU sec, 3.595 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281328, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 355, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (366.94, 162.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 82, #reads = 1082782 mem = 536878795 peak = 1008558080
[M::mem_process_seqs] Processed 675412 reads in 110.395 CPU sec, 3.653 real sec
[M::process] read 678070 sequences (100000195 bp) in 3.583 CPU sec, 3.946 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281878, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (351.20, 161.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678070 reads in 112.396 CPU sec, 3.732 real sec
[M::process] read 677328 sequences (100000147 bp) in 3.641 CPU sec, 4.027 real sec...
sorting block 83, #reads = 1082193 mem = 536884380 peak = 1008558080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282339, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 344, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (356.72, 161.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678264 sequences (100000228 bp) in 2.889 CPU sec, 3.093 real sec...
[M::mem_process_seqs] Processed 677328 reads in 112.699 CPU sec, 3.693 real sec
sorting block 84, #reads = 1084154 mem = 536878617 peak = 1015459840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282807, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.78, 161.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678264 reads in 112.552 CPU sec, 3.699 real sec
[M::process] read 677972 sequences (100000155 bp) in 3.939 CPU sec, 4.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282398, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (349.47, 159.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677972 reads in 105.411 CPU sec, 3.480 real sec
[M::process] read 678184 sequences (100000196 bp) in 3.525 CPU sec, 3.809 real sec...
sorting block 85, #reads = 1083595 mem = 536878398 peak = 1015459840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282817, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (348.42, 159.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678184 reads in 112.682 CPU sec, 3.678 real sec
[M::process] read 677756 sequences (100000247 bp) in 3.852 CPU sec, 4.281 real sec...
sorting block 86, #reads = 1081810 mem = 536879631 peak = 1015459840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282306, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (350.01, 160.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677900 sequences (100000218 bp) in 3.409 CPU sec, 3.675 real sec...
[M::mem_process_seqs] Processed 677756 reads in 112.997 CPU sec, 3.684 real sec
[M::process] read 677676 sequences (100000145 bp) in 2.531 CPU sec, 2.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282485, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (351.66, 161.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677596 sequences (100000096 bp) in 2.117 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282081, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (351.31, 160.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677900 reads in 196.028 CPU sec, 6.211 real sec
sorting block 87, #reads = 1084199 mem = 536878905 peak = 1015459840
[M::mem_process_seqs] Processed 677676 reads in 128.371 CPU sec, 4.056 real sec
[M::process] read 676480 sequences (100000204 bp) in 3.096 CPU sec, 3.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282187, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.32, 162.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 88, #reads = 1083532 mem = 536883016 peak = 1015459840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282152, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 675572 sequences (100000238 bp) in 3.375 CPU sec, 3.588 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (230, 345, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (359.63, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677596 reads in 190.227 CPU sec, 6.096 real sec
[M::mem_process_seqs] Processed 676480 reads in 127.841 CPU sec, 4.067 real sec
sorting block 89, #reads = 1082851 mem = 536880860 peak = 1015459840
[M::process] read 676342 sequences (100000121 bp) in 3.416 CPU sec, 3.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281867, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (364.61, 163.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281729, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (359.04, 162.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677590 sequences (100000201 bp) in 3.319 CPU sec, 3.516 real sec...
[M::mem_process_seqs] Processed 675572 reads in 197.111 CPU sec, 6.289 real sec
[M::mem_process_seqs] Processed 676342 reads in 130.883 CPU sec, 4.155 real sec
[M::process] read 677344 sequences (100000260 bp) in 3.682 CPU sec, 3.965 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282671, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 339, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.27, 162.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 90, #reads = 1082009 mem = 536878665 peak = 1015459840
[M::mem_process_seqs] Processed 677590 reads in 107.071 CPU sec, 3.568 real sec
[M::process] read 677164 sequences (100000292 bp) in 3.401 CPU sec, 3.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281787, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (352.14, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 91, #reads = 1083679 mem = 536879652 peak = 1015459840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282022, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (356.11, 163.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677344 reads in 202.976 CPU sec, 6.527 real sec
[M::process] read 675932 sequences (100000296 bp) in 3.390 CPU sec, 3.587 real sec...
[M::mem_process_seqs] Processed 677164 reads in 132.538 CPU sec, 4.225 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281244, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (363.87, 163.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675932 reads in 108.404 CPU sec, 3.619 real sec
[M::process] read 676850 sequences (100000248 bp) in 4.023 CPU sec, 4.506 real sec...
sorting block 92, #reads = 1082566 mem = 536884861 peak = 1015459840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282350, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 342, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (357.21, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676850 reads in 114.329 CPU sec, 3.796 real sec
[M::process] read 677082 sequences (100000033 bp) in 3.661 CPU sec, 3.958 real sec...
sorting block 93, #reads = 1082644 mem = 536879694 peak = 1015459840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282390, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (353.99, 161.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677082 reads in 115.909 CPU sec, 3.855 real sec
[M::process] read 677264 sequences (100000100 bp) in 4.006 CPU sec, 4.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281982, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (352.38, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 94, #reads = 1082702 mem = 536878957 peak = 1015459840
[M::mem_process_seqs] Processed 677264 reads in 120.073 CPU sec, 3.968 real sec
[M::process] read 677418 sequences (100000098 bp) in 3.944 CPU sec, 4.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282253, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.50, 160.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677418 reads in 117.527 CPU sec, 3.879 real sec
[M::process] read 677464 sequences (100000296 bp) in 3.619 CPU sec, 4.188 real sec...
sorting block 95, #reads = 1082104 mem = 536879498 peak = 1015459840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282045, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (346.86, 159.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677406 sequences (100000167 bp) in 3.291 CPU sec, 3.657 real sec...
[M::mem_process_seqs] Processed 677464 reads in 116.013 CPU sec, 3.840 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282177, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (349.59, 160.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677724 sequences (100000016 bp) in 3.231 CPU sec, 3.576 real sec...
[M::mem_process_seqs] Processed 677406 reads in 112.569 CPU sec, 3.738 real sec
sorting block 96, #reads = 1082793 mem = 536882367 peak = 1015459840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283081, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (346.75, 158.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677724 reads in 109.872 CPU sec, 3.657 real sec
[M::process] read 677608 sequences (100000120 bp) in 3.660 CPU sec, 3.952 real sec...
sorting block 97, #reads = 1082117 mem = 536879201 peak = 1015459840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282470, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (348.46, 160.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677608 reads in 110.242 CPU sec, 3.654 real sec
[M::process] read 677592 sequences (100000070 bp) in 3.497 CPU sec, 3.807 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282481, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (348.10, 160.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677592 reads in 110.057 CPU sec, 3.666 real sec
[M::process] read 677346 sequences (100000032 bp) in 3.784 CPU sec, 4.204 real sec...
sorting block 98, #reads = 1082395 mem = 536882174 peak = 1015459840
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282531, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (351.57, 162.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677346 reads in 112.083 CPU sec, 3.715 real sec
[M::process] read 677330 sequences (100000257 bp) in 3.672 CPU sec, 3.992 real sec...
sorting block 99, #reads = 1082062 mem = 536878965 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281891, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (351.71, 162.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677330 reads in 112.879 CPU sec, 3.711 real sec
[M::process] read 677282 sequences (100000244 bp) in 3.444 CPU sec, 3.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282485, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (352.81, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677332 sequences (100000194 bp) in 3.236 CPU sec, 3.676 real sec...
[M::mem_process_seqs] Processed 677282 reads in 114.179 CPU sec, 3.767 real sec
sorting block 100, #reads = 1081952 mem = 536878507 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282248, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.86, 162.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677332 reads in 113.518 CPU sec, 3.747 real sec
[M::process] read 678110 sequences (100000279 bp) in 3.794 CPU sec, 4.034 real sec...
sorting block 101, #reads = 1081482 mem = 536880791 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282873, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (348.93, 164.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678110 reads in 113.967 CPU sec, 3.756 real sec
[M::process] read 681990 sequences (100000024 bp) in 3.648 CPU sec, 3.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284497, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 309, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (335.26, 165.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678520 sequences (100000072 bp) in 3.579 CPU sec, 3.818 real sec...
[M::mem_process_seqs] Processed 681990 reads in 125.580 CPU sec, 4.107 real sec
sorting block 102, #reads = 1082005 mem = 536878312 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283031, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 343, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (360.35, 171.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678520 reads in 124.592 CPU sec, 4.044 real sec
[M::process] read 678772 sequences (100000145 bp) in 4.134 CPU sec, 4.601 real sec...
sorting block 103, #reads = 1079932 mem = 536878366 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283093, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 341, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (359.02, 171.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678772 reads in 115.068 CPU sec, 3.739 real sec
[M::process] read 678844 sequences (100000180 bp) in 3.726 CPU sec, 4.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283360, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (357.57, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678844 reads in 122.259 CPU sec, 3.960 real sec
[M::process] read 680376 sequences (100000177 bp) in 3.657 CPU sec, 4.050 real sec...
sorting block 104, #reads = 1079386 mem = 536879678 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283770, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 322, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (342.84, 164.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679902 sequences (100000288 bp) in 3.417 CPU sec, 3.674 real sec...
[M::mem_process_seqs] Processed 680376 reads in 119.451 CPU sec, 3.866 real sec
sorting block 105, #reads = 1081405 mem = 536879160 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283745, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 325, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (345.33, 165.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679902 reads in 121.143 CPU sec, 3.917 real sec
[M::process] read 680274 sequences (100000023 bp) in 3.838 CPU sec, 4.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283420, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 325, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (345.75, 166.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680274 reads in 115.593 CPU sec, 3.742 real sec
[M::process] read 680446 sequences (100000091 bp) in 3.696 CPU sec, 4.084 real sec...
sorting block 106, #reads = 1082784 mem = 536879707 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284275, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (339.77, 162.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678828 sequences (100000270 bp) in 3.157 CPU sec, 3.514 real sec...
[M::mem_process_seqs] Processed 680446 reads in 124.112 CPU sec, 4.011 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283087, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 334, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (352.63, 166.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 107, #reads = 1082384 mem = 536877688 peak = 1060167680
[M::mem_process_seqs] Processed 678828 reads in 116.681 CPU sec, 3.783 real sec
[M::process] read 677160 sequences (100000242 bp) in 4.036 CPU sec, 4.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282526, 13, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 351, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (364.74, 168.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 181, 181)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (181, 181)
[M::mem_pestat] mean and std.dev: (181.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (181, 181)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677160 reads in 119.517 CPU sec, 3.885 real sec
[M::process] read 679284 sequences (100000155 bp) in 3.967 CPU sec, 4.206 real sec...
sorting block 108, #reads = 1079862 mem = 536878702 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283793, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (347.44, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679284 reads in 118.571 CPU sec, 3.840 real sec
[M::process] read 680032 sequences (100000299 bp) in 3.597 CPU sec, 3.883 real sec...
[M::process] read 680654 sequences (100000249 bp) in 2.873 CPU sec, 3.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283623, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 324, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (344.35, 165.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679878 sequences (100000112 bp) in 2.272 CPU sec, 2.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283638, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (337.21, 160.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680032 reads in 214.244 CPU sec, 6.821 real sec
sorting block 109, #reads = 1080782 mem = 536883034 peak = 1060167680
[M::mem_process_seqs] Processed 680654 reads in 139.260 CPU sec, 4.399 real sec
[M::process] read 679204 sequences (100000059 bp) in 2.760 CPU sec, 2.814 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283981, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 328, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (345.74, 163.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 110, #reads = 1080408 mem = 536886514 peak = 1060167680
[M::process] read 678632 sequences (100000000 bp) in 3.420 CPU sec, 3.708 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283507, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (350.09, 164.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679878 reads in 211.371 CPU sec, 6.745 real sec
[M::mem_process_seqs] Processed 679204 reads in 142.071 CPU sec, 4.496 real sec
[M::process] read 676492 sequences (100000098 bp) in 2.866 CPU sec, 3.054 real sec...
sorting block 111, #reads = 1079743 mem = 536878852 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283274, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (350.30, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677026 sequences (100000247 bp) in 3.257 CPU sec, 3.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282216, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 353, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (366.65, 168.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678244 sequences (100000038 bp) in 1.820 CPU sec, 1.821 real sec...
[M::mem_process_seqs] Processed 678632 reads in 213.701 CPU sec, 6.813 real sec
[M::mem_process_seqs] Processed 676492 reads in 143.738 CPU sec, 4.569 real sec
sorting block 112, #reads = 1080219 mem = 536879189 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282604, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 348, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (362.47, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678474 sequences (100000264 bp) in 3.990 CPU sec, 4.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283257, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (352.60, 164.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677026 reads in 213.454 CPU sec, 6.790 real sec
[M::process] read 676348 sequences (100000236 bp) in 2.438 CPU sec, 2.503 real sec...
[M::mem_process_seqs] Processed 678244 reads in 143.225 CPU sec, 4.527 real sec
sorting block 113, #reads = 1078150 mem = 536880184 peak = 1060167680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283548, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (352.01, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677720 sequences (100000117 bp) in 3.724 CPU sec, 3.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282204, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 350, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (364.46, 166.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678474 reads in 207.937 CPU sec, 6.644 real sec
sorting block 114, #reads = 1079864 mem = 536879009 peak = 1084133376
[M::mem_process_seqs] Processed 676348 reads in 139.886 CPU sec, 4.430 real sec
[M::process] read 677154 sequences (100000098 bp) in 3.602 CPU sec, 3.801 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283463, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (349.12, 159.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 115, #reads = 1080111 mem = 536879042 peak = 1084133376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282637, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (352.93, 160.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677578 sequences (100000102 bp) in 3.887 CPU sec, 4.200 real sec...
[M::mem_process_seqs] Processed 677720 reads in 204.325 CPU sec, 6.545 real sec
[M::mem_process_seqs] Processed 677154 reads in 136.436 CPU sec, 4.337 real sec
sorting block 116, #reads = 1079466 mem = 536878032 peak = 1084133376
[M::process] read 676574 sequences (100000174 bp) in 3.723 CPU sec, 3.969 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282938, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (352.43, 162.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282304, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 344, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (359.79, 164.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677578 reads in 202.748 CPU sec, 6.495 real sec
[M::process] read 675792 sequences (100000248 bp) in 3.638 CPU sec, 4.109 real sec...
[M::mem_process_seqs] Processed 676574 reads in 135.993 CPU sec, 4.335 real sec
sorting block 117, #reads = 1078564 mem = 536878196 peak = 1084133376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282341, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 354, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (367.20, 165.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675792 reads in 120.878 CPU sec, 3.995 real sec
[M::process] read 677130 sequences (100000186 bp) in 4.306 CPU sec, 4.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282557, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 339, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (355.09, 163.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 118, #reads = 1079044 mem = 536880401 peak = 1084133376
[M::mem_process_seqs] Processed 677130 reads in 125.085 CPU sec, 4.142 real sec
[M::process] read 677670 sequences (100000077 bp) in 4.050 CPU sec, 4.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282714, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (349.13, 160.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677670 reads in 121.420 CPU sec, 3.964 real sec
sorting block 119, #reads = 1077687 mem = 536878718 peak = 1084133376
[M::process] read 677674 sequences (100000048 bp) in 3.985 CPU sec, 4.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282842, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (349.41, 161.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677674 reads in 123.147 CPU sec, 3.988 real sec
[M::process] read 676458 sequences (100000246 bp) in 3.876 CPU sec, 4.177 real sec...
sorting block 120, #reads = 1079153 mem = 536878118 peak = 1084133376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282454, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 346, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (360.85, 164.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676458 reads in 119.339 CPU sec, 3.874 real sec
[M::process] read 677136 sequences (100000062 bp) in 3.962 CPU sec, 4.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282824, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 339, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (356.55, 165.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677136 reads in 117.837 CPU sec, 3.825 real sec
sorting block 121, #reads = 1078246 mem = 536879784 peak = 1084133376
[M::process] read 677378 sequences (100000152 bp) in 4.157 CPU sec, 4.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282850, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (354.72, 164.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677288 sequences (100000076 bp) in 3.013 CPU sec, 3.317 real sec...
[M::mem_process_seqs] Processed 677378 reads in 119.708 CPU sec, 3.866 real sec
sorting block 122, #reads = 1079626 mem = 536881193 peak = 1084133376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282741, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (355.01, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677288 reads in 119.860 CPU sec, 3.879 real sec
[M::process] read 677306 sequences (100000212 bp) in 4.140 CPU sec, 4.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282264, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 338, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (356.65, 166.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676168 sequences (100000181 bp) in 3.356 CPU sec, 3.510 real sec...
[M::mem_process_seqs] Processed 677306 reads in 118.010 CPU sec, 3.810 real sec
sorting block 123, #reads = 1079648 mem = 536878799 peak = 1084133376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282567, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 350, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (366.54, 169.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682236 sequences (100000057 bp) in 3.266 CPU sec, 3.440 real sec...
[M::mem_process_seqs] Processed 676168 reads in 118.480 CPU sec, 3.814 real sec
sorting block 124, #reads = 1078981 mem = 536877268 peak = 1084133376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284986, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 310, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (331.36, 159.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682850 sequences (100000270 bp) in 4.026 CPU sec, 4.305 real sec...
[M::mem_process_seqs] Processed 682236 reads in 130.647 CPU sec, 4.204 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284830, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 305, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (325.53, 155.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681202 sequences (100000282 bp) in 4.001 CPU sec, 4.318 real sec...
[M::mem_process_seqs] Processed 682850 reads in 139.761 CPU sec, 4.495 real sec
sorting block 125, #reads = 1075822 mem = 536880114 peak = 1084133376
[M::process] read 681220 sequences (100000094 bp) in 3.556 CPU sec, 3.694 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284545, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (335.73, 157.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680876 sequences (100000150 bp) in 2.500 CPU sec, 2.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284760, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (333.01, 155.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681202 reads in 234.705 CPU sec, 7.379 real sec
[M::mem_process_seqs] Processed 681220 reads in 153.902 CPU sec, 4.843 real sec
[M::process] read 680510 sequences (100000161 bp) in 2.695 CPU sec, 2.776 real sec...
sorting block 126, #reads = 1073496 mem = 536878351 peak = 1084133376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284964, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 318, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (334.81, 156.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680664 sequences (100000035 bp) in 4.265 CPU sec, 4.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284814, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (337.49, 158.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680876 reads in 220.261 CPU sec, 7.027 real sec
[M::mem_process_seqs] Processed 680510 reads in 146.659 CPU sec, 4.642 real sec
[M::process] read 680302 sequences (100000166 bp) in 2.376 CPU sec, 2.460 real sec...
sorting block 127, #reads = 1075234 mem = 536877657 peak = 1084133376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284693, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (334.42, 156.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678510 sequences (100000026 bp) in 2.950 CPU sec, 3.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284006, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 680190 sequences (100000037 bp) in 2.380 CPU sec, 2.385 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (210, 318, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (335.64, 156.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680664 reads in 225.359 CPU sec, 7.158 real sec
sorting block 128, #reads = 1077137 mem = 536878770 peak = 1084133376
[M::mem_process_seqs] Processed 680302 reads in 151.435 CPU sec, 4.810 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283220, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (349.81, 161.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 129, #reads = 1076793 mem = 536878329 peak = 1131126784
[M::process] read 680208 sequences (100000183 bp) in 3.836 CPU sec, 4.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284544, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (337.86, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678510 reads in 216.885 CPU sec, 6.892 real sec
[M::mem_process_seqs] Processed 680190 reads in 142.121 CPU sec, 4.493 real sec
[M::process] read 680360 sequences (100000012 bp) in 3.042 CPU sec, 3.166 real sec...
sorting block 130, #reads = 1077175 mem = 536878752 peak = 1131126784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284231, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 318, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (336.27, 157.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680174 sequences (100000233 bp) in 3.512 CPU sec, 3.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284388, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 320, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (337.72, 159.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680208 reads in 215.730 CPU sec, 6.885 real sec
[M::process] read 679498 sequences (100000018 bp) in 1.946 CPU sec, 1.958 real sec...
[M::mem_process_seqs] Processed 680360 reads in 144.676 CPU sec, 4.592 real sec
sorting block 131, #reads = 1079144 mem = 536876738 peak = 1131126784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284227, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (338.72, 159.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678682 sequences (100000271 bp) in 3.669 CPU sec, 3.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284068, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (343.14, 161.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680174 reads in 221.112 CPU sec, 7.031 real sec
[M::process] read 676500 sequences (100000008 bp) in 2.751 CPU sec, 2.777 real sec...
[M::mem_process_seqs] Processed 679498 reads in 146.743 CPU sec, 4.646 real sec
sorting block 132, #reads = 1078883 mem = 536877107 peak = 1131126784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283145, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (350.15, 162.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678518 sequences (100000222 bp) in 3.682 CPU sec, 3.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282395, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 354, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (366.55, 167.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678682 reads in 214.863 CPU sec, 6.868 real sec
sorting block 133, #reads = 1078461 mem = 536880326 peak = 1131126784
[M::mem_process_seqs] Processed 676500 reads in 142.955 CPU sec, 4.527 real sec
[M::process] read 678368 sequences (100000068 bp) in 3.930 CPU sec, 4.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283215, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 338, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (354.61, 166.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 134, #reads = 1078551 mem = 536879767 peak = 1131126784
[M::process] read 677626 sequences (100000261 bp) in 3.671 CPU sec, 3.878 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284113, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (351.01, 164.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678518 reads in 208.335 CPU sec, 6.672 real sec
[M::mem_process_seqs] Processed 678368 reads in 138.223 CPU sec, 4.385 real sec
[M::process] read 677810 sequences (100000090 bp) in 3.162 CPU sec, 3.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282889, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (359.75, 168.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 135, #reads = 1079422 mem = 536881380 peak = 1131126784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283124, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (354.42, 164.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677658 sequences (100000271 bp) in 4.132 CPU sec, 4.471 real sec...
[M::mem_process_seqs] Processed 677626 reads in 206.049 CPU sec, 6.605 real sec
[M::mem_process_seqs] Processed 677810 reads in 139.757 CPU sec, 4.458 real sec
sorting block 136, #reads = 1078980 mem = 536879302 peak = 1131126784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282898, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 339, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (355.56, 165.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677558 sequences (100000053 bp) in 4.245 CPU sec, 4.621 real sec...
[M::mem_process_seqs] Processed 677658 reads in 112.544 CPU sec, 3.723 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282676, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 340, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (357.41, 166.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677558 reads in 118.756 CPU sec, 3.919 real sec
sorting block 137, #reads = 1079121 mem = 536879003 peak = 1131126784
[M::process] read 677264 sequences (100000218 bp) in 3.687 CPU sec, 4.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283277, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (357.78, 166.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677264 reads in 115.360 CPU sec, 3.808 real sec
[M::process] read 675672 sequences (100000132 bp) in 3.687 CPU sec, 4.071 real sec...
sorting block 138, #reads = 1078887 mem = 536880445 peak = 1131126784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282646, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 358, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (372.01, 169.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675672 reads in 122.698 CPU sec, 4.032 real sec
[M::process] read 676542 sequences (100000136 bp) in 4.024 CPU sec, 4.482 real sec...
sorting block 139, #reads = 1079127 mem = 536880060 peak = 1131126784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282592, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 349, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (364.24, 168.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676542 reads in 120.672 CPU sec, 3.955 real sec
[M::process] read 677718 sequences (100000170 bp) in 3.585 CPU sec, 3.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283226, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (355.39, 166.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677718 reads in 123.223 CPU sec, 4.017 real sec
[M::process] read 677674 sequences (100000298 bp) in 4.388 CPU sec, 4.895 real sec...
sorting block 140, #reads = 1078698 mem = 536878860 peak = 1131126784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283725, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (354.48, 166.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677402 sequences (100000276 bp) in 3.553 CPU sec, 3.772 real sec...
[M::mem_process_seqs] Processed 677674 reads in 118.849 CPU sec, 3.866 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283172, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 339, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (358.39, 168.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 141, #reads = 1080144 mem = 536877031 peak = 1131126784
[M::mem_process_seqs] Processed 677402 reads in 119.890 CPU sec, 3.875 real sec
[M::process] read 677396 sequences (100000026 bp) in 4.368 CPU sec, 4.814 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283210, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (361.46, 171.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677396 reads in 122.790 CPU sec, 3.990 real sec
[M::process] read 677642 sequences (100000138 bp) in 3.812 CPU sec, 4.151 real sec...
sorting block 142, #reads = 1078654 mem = 536878310 peak = 1131126784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283020, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (360.03, 171.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677642 reads in 122.411 CPU sec, 3.971 real sec
[M::process] read 677596 sequences (100000241 bp) in 3.864 CPU sec, 4.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283285, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (359.89, 171.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 143, #reads = 1079250 mem = 536885322 peak = 1131126784
[M::mem_process_seqs] Processed 677596 reads in 125.870 CPU sec, 4.095 real sec
[M::process] read 677648 sequences (100000102 bp) in 4.311 CPU sec, 4.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282449, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677732 sequences (100000130 bp) in 3.186 CPU sec, 3.378 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (357.61, 170.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681698 sequences (100000021 bp) in 2.530 CPU sec, 2.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283072, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (356.59, 169.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677648 reads in 248.964 CPU sec, 7.886 real sec
[M::process] read 679948 sequences (100000140 bp) in 2.445 CPU sec, 2.462 real sec...
[M::mem_process_seqs] Processed 677732 reads in 175.161 CPU sec, 5.528 real sec
sorting block 144, #reads = 1078214 mem = 536880674 peak = 1131126784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284476, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 313, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (330.14, 155.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679886 sequences (100000004 bp) in 3.692 CPU sec, 4.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283837, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (342.90, 160.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681698 reads in 217.970 CPU sec, 6.939 real sec
sorting block 145, #reads = 1068817 mem = 536878386 peak = 1131126784
[M::mem_process_seqs] Processed 679948 reads in 143.053 CPU sec, 4.527 real sec
[M::process] read 679456 sequences (100000007 bp) in 3.361 CPU sec, 3.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284242, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (343.01, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 146, #reads = 1080128 mem = 536879113 peak = 1141133312
[M::process] read 679344 sequences (100000277 bp) in 3.313 CPU sec, 3.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283646, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (343.03, 160.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679886 reads in 207.210 CPU sec, 6.609 real sec
[M::mem_process_seqs] Processed 679456 reads in 136.243 CPU sec, 4.322 real sec
[M::process] read 679164 sequences (100000295 bp) in 3.742 CPU sec, 3.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283827, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (344.44, 159.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 147, #reads = 1081377 mem = 536879416 peak = 1141133312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283915, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (347.53, 162.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679344 reads in 199.844 CPU sec, 6.429 real sec
[M::process] read 678694 sequences (100000063 bp) in 4.009 CPU sec, 4.319 real sec...
[M::mem_process_seqs] Processed 679164 reads in 134.271 CPU sec, 4.284 real sec
sorting block 148, #reads = 1081739 mem = 536879446 peak = 1141133312
[M::process] read 676552 sequences (100000231 bp) in 3.406 CPU sec, 3.679 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283347, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (350.18, 162.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282940, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (361.13, 161.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678694 reads in 201.359 CPU sec, 6.487 real sec
[M::mem_process_seqs] Processed 676552 reads in 130.101 CPU sec, 4.166 real sec
[M::process] read 677606 sequences (100000005 bp) in 3.952 CPU sec, 4.330 real sec...
sorting block 149, #reads = 1081631 mem = 536880189 peak = 1141133312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283278, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (355.34, 161.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677606 reads in 103.409 CPU sec, 3.449 real sec
[M::process] read 679956 sequences (100000001 bp) in 3.745 CPU sec, 4.238 real sec...
sorting block 150, #reads = 1080499 mem = 536879833 peak = 1141133312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283582, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (338.43, 157.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679956 reads in 99.123 CPU sec, 3.274 real sec
[M::process] read 679792 sequences (100000157 bp) in 3.001 CPU sec, 3.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283608, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 319, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
sorting block 151, #reads = 1080869 mem = 536876850 peak = 1141133312
[M::mem_pestat] mean and std.dev: (335.74, 155.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679792 reads in 109.123 CPU sec, 3.549 real sec
[M::process] read 680244 sequences (100000168 bp) in 3.334 CPU sec, 3.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283708, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (334.20, 154.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679930 sequences (100000223 bp) in 2.992 CPU sec, 3.250 real sec...
[M::mem_process_seqs] Processed 680244 reads in 118.445 CPU sec, 3.866 real sec
sorting block 152, #reads = 1081716 mem = 536879144 peak = 1141133312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283911, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 319, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (335.75, 155.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679654 sequences (100000166 bp) in 4.034 CPU sec, 4.412 real sec...
[M::mem_process_seqs] Processed 679930 reads in 122.152 CPU sec, 3.966 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283798, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 321, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (337.53, 156.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 153, #reads = 1080232 mem = 536878216 peak = 1141133312
[M::mem_process_seqs] Processed 679654 reads in 111.496 CPU sec, 3.600 real sec
[M::process] read 678914 sequences (100000083 bp) in 4.183 CPU sec, 4.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283870, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 326, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (342.73, 158.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678916 sequences (100000273 bp) in 3.480 CPU sec, 3.757 real sec...
[M::mem_process_seqs] Processed 678914 reads in 117.372 CPU sec, 3.797 real sec
sorting block 154, #reads = 1081019 mem = 536880077 peak = 1141133312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283434, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 326, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (342.43, 158.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678916 reads in 111.405 CPU sec, 3.625 real sec
[M::process] read 679088 sequences (100000199 bp) in 3.739 CPU sec, 4.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283462, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (344.65, 160.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 155, #reads = 1081892 mem = 536886355 peak = 1141133312
[M::mem_process_seqs] Processed 679088 reads in 120.839 CPU sec, 3.916 real sec
[M::process] read 677420 sequences (100000042 bp) in 3.938 CPU sec, 4.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282866, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 343, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (356.30, 161.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677028 sequences (100000072 bp) in 3.268 CPU sec, 3.502 real sec...
[M::mem_process_seqs] Processed 677420 reads in 119.072 CPU sec, 3.849 real sec
sorting block 156, #reads = 1079853 mem = 536877369 peak = 1141133312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283334, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 344, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (356.57, 161.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678966 sequences (100000183 bp) in 3.591 CPU sec, 3.834 real sec...
[M::mem_process_seqs] Processed 677028 reads in 117.500 CPU sec, 3.774 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283541, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (342.56, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678868 sequences (100000245 bp) in 3.474 CPU sec, 3.676 real sec...
[M::mem_process_seqs] Processed 678966 reads in 113.459 CPU sec, 3.668 real sec
sorting block 157, #reads = 1079991 mem = 536882282 peak = 1141133312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283249, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (342.95, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678868 reads in 120.373 CPU sec, 3.914 real sec
[M::process] read 678944 sequences (100000191 bp) in 4.043 CPU sec, 4.427 real sec...
sorting block 158, #reads = 1081921 mem = 536878674 peak = 1141133312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283406, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (344.17, 159.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678944 reads in 119.865 CPU sec, 3.895 real sec
[M::process] read 678402 sequences (100000142 bp) in 3.987 CPU sec, 4.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283029, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (346.20, 159.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678402 reads in 117.881 CPU sec, 3.810 real sec
[M::process] read 678330 sequences (100000157 bp) in 3.684 CPU sec, 3.917 real sec...
sorting block 159, #reads = 1080317 mem = 536879141 peak = 1141133312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283126, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (347.90, 160.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678330 reads in 125.781 CPU sec, 4.053 real sec
[M::process] read 677908 sequences (100000132 bp) in 3.808 CPU sec, 4.079 real sec...
sorting block 160, #reads = 1079768 mem = 536879393 peak = 1141133312
[M::process] read 677988 sequences (100000147 bp) in 2.645 CPU sec, 2.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282860, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (351.83, 162.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677768 sequences (100000105 bp) in 1.900 CPU sec, 1.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281936, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (352.22, 162.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678094 sequences (100000282 bp) in 1.800 CPU sec, 1.793 real sec...
[M::mem_process_seqs] Processed 677908 reads in 224.945 CPU sec, 7.102 real sec
[M::mem_process_seqs] Processed 677988 reads in 151.474 CPU sec, 4.798 real sec
sorting block 161, #reads = 1078963 mem = 536878064 peak = 1141133312
[M::process] read 678032 sequences (100000032 bp) in 3.803 CPU sec, 4.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283339, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (353.14, 165.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678094 reads in 121.802 CPU sec, 3.836 real sec
[M::process] read 677966 sequences (100000069 bp) in 2.778 CPU sec, 2.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282896, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (352.68, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283366, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 336, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (353.78, 165.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677768 reads in 338.325 CPU sec, 10.743 real sec
sorting block 162, #reads = 1078507 mem = 536879552 peak = 1141133312
[M::process] read 677722 sequences (100000000 bp) in 2.748 CPU sec, 2.768 real sec...
[M::mem_process_seqs] Processed 678032 reads in 169.041 CPU sec, 5.364 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282857, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (353.76, 164.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 163, #reads = 1078135 mem = 536883072 peak = 1181384704
[M::process] read 678268 sequences (100000051 bp) in 4.105 CPU sec, 4.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282809, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 338, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (355.92, 165.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677966 reads in 211.771 CPU sec, 6.746 real sec
[M::mem_process_seqs] Processed 677722 reads in 130.249 CPU sec, 4.112 real sec
[M::process] read 678212 sequences (100000122 bp) in 3.552 CPU sec, 3.730 real sec...
sorting block 164, #reads = 1077674 mem = 536878766 peak = 1181384704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282785, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (348.93, 164.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282616, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 678878 sequences (100000048 bp) in 3.252 CPU sec, 3.429 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (351.07, 166.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678268 reads in 187.515 CPU sec, 6.012 real sec
[M::mem_process_seqs] Processed 678212 reads in 115.129 CPU sec, 3.647 real sec
sorting block 165, #reads = 1076867 mem = 536876619 peak = 1181384704
[M::process] read 679536 sequences (100000158 bp) in 3.122 CPU sec, 3.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283348, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 322, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (344.85, 165.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683120 sequences (100000000 bp) in 2.312 CPU sec, 2.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283317, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (343.54, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678878 reads in 195.781 CPU sec, 6.262 real sec
sorting block 166, #reads = 1076789 mem = 536878370 peak = 1181384704
[M::process] read 681278 sequences (100000096 bp) in 1.965 CPU sec, 2.000 real sec...
[M::mem_process_seqs] Processed 679536 reads in 126.654 CPU sec, 3.992 real sec
sorting block 167, #reads = 1070864 mem = 536878965 peak = 1181384704
[M::process] read 680736 sequences (100000194 bp) in 2.550 CPU sec, 2.737 real sec...
[M::process] read 681844 sequences (100000051 bp) in 1.840 CPU sec, 1.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284670, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 320, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (332.63, 152.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681278 reads in 96.950 CPU sec, 3.062 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284923, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 307, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (323.79, 152.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284086, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 320, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (334.18, 152.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682248 sequences (100000243 bp) in 1.903 CPU sec, 1.910 real sec...
[M::mem_process_seqs] Processed 683120 reads in 282.655 CPU sec, 8.995 real sec
sorting block 168, #reads = 1073826 mem = 536880886 peak = 1181384704
[M::mem_process_seqs] Processed 680736 reads in 131.189 CPU sec, 4.200 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284178, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 311, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (326.49, 150.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679256 sequences (100000051 bp) in 3.361 CPU sec, 3.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285007, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 307, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (323.59, 149.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681844 reads in 181.415 CPU sec, 5.758 real sec
sorting block 169, #reads = 1073216 mem = 536879768 peak = 1181384704
[M::mem_process_seqs] Processed 682248 reads in 116.133 CPU sec, 3.669 real sec
[M::process] read 679044 sequences (100000282 bp) in 2.884 CPU sec, 2.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283691, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (344.24, 155.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 170, #reads = 1074720 mem = 536879017 peak = 1181384704
[M::process] read 682104 sequences (100000240 bp) in 2.891 CPU sec, 3.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283844, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (347.37, 156.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679256 reads in 175.477 CPU sec, 5.593 real sec
[M::mem_process_seqs] Processed 679044 reads in 113.503 CPU sec, 3.586 real sec
[M::process] read 681142 sequences (100000127 bp) in 2.240 CPU sec, 2.321 real sec...
sorting block 171, #reads = 1074803 mem = 536877820 peak = 1181384704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284658, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 311, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (328.33, 153.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681748 sequences (100000093 bp) in 3.180 CPU sec, 3.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283937, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (333.87, 156.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682104 reads in 169.039 CPU sec, 5.404 real sec
[M::mem_process_seqs] Processed 681142 reads in 109.018 CPU sec, 3.455 real sec
sorting block 172, #reads = 1076644 mem = 536879521 peak = 1181384704
[M::process] read 681868 sequences (100000124 bp) in 2.708 CPU sec, 2.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284642, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 309, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (326.91, 152.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681302 sequences (100000012 bp) in 3.100 CPU sec, 3.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284167, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 304, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (321.31, 148.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681748 reads in 171.956 CPU sec, 5.504 real sec
[M::mem_process_seqs] Processed 681868 reads in 113.907 CPU sec, 3.615 real sec
sorting block 173, #reads = 1078492 mem = 536878366 peak = 1181384704
[M::process] read 680692 sequences (100000221 bp) in 2.757 CPU sec, 2.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284454, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (323.22, 147.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 174, #reads = 1077111 mem = 536877871 peak = 1181384704
[M::process] read 680298 sequences (100000076 bp) in 2.748 CPU sec, 2.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284291, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 313, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (328.86, 150.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681302 reads in 167.862 CPU sec, 5.374 real sec
[M::process] read 678718 sequences (100000064 bp) in 2.394 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284141, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 316, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (331.25, 151.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680298 reads in 92.380 CPU sec, 2.924 real sec
[M::process] read 678170 sequences (100000276 bp) in 2.179 CPU sec, 2.240 real sec...
sorting block 175, #reads = 1077798 mem = 536877635 peak = 1181384704
[M::mem_process_seqs] Processed 680692 reads in 237.674 CPU sec, 7.540 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283406, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (342.70, 154.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679782 sequences (100000047 bp) in 2.789 CPU sec, 2.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283238, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (344.70, 154.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678718 reads in 181.154 CPU sec, 5.748 real sec
sorting block 176, #reads = 1079228 mem = 536878425 peak = 1181384704
[M::mem_process_seqs] Processed 678170 reads in 106.598 CPU sec, 3.376 real sec
[M::process] read 679574 sequences (100000197 bp) in 2.490 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283390, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 320, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (335.12, 153.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 177, #reads = 1077736 mem = 536876749 peak = 1181384704
[M::process] read 679792 sequences (100000211 bp) in 2.592 CPU sec, 2.726 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283107, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 320, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (335.88, 154.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679782 reads in 165.573 CPU sec, 5.271 real sec
[M::mem_process_seqs] Processed 679574 reads in 107.529 CPU sec, 3.398 real sec
[M::process] read 678540 sequences (100000179 bp) in 2.075 CPU sec, 2.119 real sec...
sorting block 178, #reads = 1078182 mem = 536879483 peak = 1181384704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283131, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 318, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (334.09, 153.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679270 sequences (100000077 bp) in 2.977 CPU sec, 3.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283709, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (344.42, 156.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679792 reads in 162.494 CPU sec, 5.176 real sec
[M::mem_process_seqs] Processed 678540 reads in 105.251 CPU sec, 3.325 real sec
[M::process] read 679278 sequences (100000001 bp) in 2.151 CPU sec, 2.217 real sec...
sorting block 179, #reads = 1078915 mem = 536877747 peak = 1181384704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283260, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (339.08, 155.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678594 sequences (100000113 bp) in 2.452 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282631, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 321, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (337.40, 154.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679270 reads in 159.731 CPU sec, 5.086 real sec
[M::process] read 678544 sequences (100000070 bp) in 2.110 CPU sec, 2.164 real sec...
sorting block 180, #reads = 1079054 mem = 536879535 peak = 1181384704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283244, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (344.14, 158.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678594 reads in 90.231 CPU sec, 2.861 real sec
[M::process] read 678560 sequences (100000138 bp) in 2.671 CPU sec, 2.743 real sec...
[M::mem_process_seqs] Processed 679278 reads in 240.295 CPU sec, 7.618 real sec
sorting block 181, #reads = 1078767 mem = 536881136 peak = 1181384704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283041, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 330, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (345.57, 158.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283109, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 326, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (341.91, 156.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678396 sequences (100000189 bp) in 2.932 CPU sec, 3.089 real sec...
[M::mem_process_seqs] Processed 678544 reads in 176.623 CPU sec, 5.623 real sec
[M::mem_process_seqs] Processed 678560 reads in 105.369 CPU sec, 3.352 real sec
sorting block 182, #reads = 1079079 mem = 536880246 peak = 1181384704
[M::process] read 678268 sequences (100000186 bp) in 2.446 CPU sec, 2.624 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283232, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (344.79, 158.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678336 sequences (100000169 bp) in 2.386 CPU sec, 2.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282787, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (345.10, 158.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678396 reads in 159.960 CPU sec, 5.095 real sec
sorting block 183, #reads = 1080211 mem = 536878117 peak = 1181384704
[M::mem_process_seqs] Processed 678268 reads in 104.002 CPU sec, 3.292 real sec
[M::process] read 678276 sequences (100000192 bp) in 2.410 CPU sec, 2.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282969, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (346.08, 159.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 184, #reads = 1080091 mem = 536880215 peak = 1183621120
[M::process] read 678240 sequences (100000021 bp) in 2.595 CPU sec, 2.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283295, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (347.86, 161.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678336 reads in 153.556 CPU sec, 4.898 real sec
[M::mem_process_seqs] Processed 678276 reads in 98.404 CPU sec, 3.109 real sec
[M::process] read 677476 sequences (100000087 bp) in 2.431 CPU sec, 2.603 real sec...
sorting block 185, #reads = 1079150 mem = 536877939 peak = 1183621120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283104, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (349.71, 163.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678548 sequences (100000161 bp) in 2.708 CPU sec, 2.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283019, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (352.00, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678240 reads in 152.711 CPU sec, 4.882 real sec
[M::process] read 243700 sequences (35869281 bp) in 0.871 CPU sec, 0.878 real sec...
[M::mem_process_seqs] Processed 677476 reads in 98.872 CPU sec, 3.138 real sec
sorting block 186, #reads = 1079302 mem = 536878037 peak = 1183621120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283215, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (347.51, 164.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 101925, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (342.57, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678548 reads in 107.415 CPU sec, 3.494 real sec
[M::mem_process_seqs] Processed 243700 reads in 45.534 CPU sec, 1.482 real sec
sorting block 187, #reads = 1079643 mem = 536878492 peak = 1213812736
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000606a6
  features: 1f8bfbff fffa320b
  extended: f1bf07ab 00407f5a
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 202953316
overall: 69881901056 mem 35410.010 user 1135.351 sys 1292.344 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cVw-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1292.381 sec; CPU: 36545.364 sec
sorting block 188, #reads = 601158 mem = 299366284 peak = 1213812736
uniq header count 1
merging 189 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000606a6
  features: 1f8bfbff fffa320b
  extended: f1bf07ab 00407f5a
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) Platinum 8375C CPU @ 2.90GHz
threads: 31 max 128
algo: util-sort
license: sentieon:util=1
output file size: 10710758856
output reads: 203829934
bam_mem_sort: 189 calls 212.612 user 6.585 sys 219.707 real
bam_write: 189 calls 94.915 user 6.737 sys 125.612 real
execute: 1 calls 51.460 user 2.240 sys 1365.050 real
merge_files: 1 calls 51.421 user 2.212 sys 71.488 real
parse_chunk: 3017 calls 191.105 user 43.938 sys 244.766 real
read_chunk: 8127 calls 99.318 user 62.827 sys 1220.714 real
sort_block: 1 calls 73.055 user 61.313 sys 1184.127 real
write_chunk: 5108 calls 50.961 user 1.746 sys 70.219 real
overall: 3344654336 mem 3087.972 user 151.353 sys 1365.071 real
ls: cannot access '*.cram': No such file or directory