This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1817.471 CPU sec, 73.346 real sec
[M::process] read 687000 sequences (100000128 bp) in 1.721 CPU sec, 1.730 real sec...
[M::process] read 685142 sequences (100000283 bp) in 1.862 CPU sec, 1.876 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279680, 3, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 250, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (276.32, 213.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 309, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (329.01, 161.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (293.91, 146.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684030 sequences (100000216 bp) in 1.948 CPU sec, 1.961 real sec...
[M::process] read 683444 sequences (100000041 bp) in 1.793 CPU sec, 1.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 279116, 12, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 180, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (31, 326)
[M::mem_pestat] mean and std.dev: (175.65, 44.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 385)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 323, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (339.46, 164.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 529, 777)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1949)
[M::mem_pestat] mean and std.dev: (446.18, 271.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2535)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 287, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (284.20, 126.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687000 reads in 219.065 CPU sec, 7.016 real sec
[M::mem_process_seqs] Processed 685142 reads in 150.540 CPU sec, 4.898 real sec
sorting block 0, #reads = 1071028 mem = 536879304 peak = 570429440
[M::process] read 682610 sequences (100000150 bp) in 2.465 CPU sec, 2.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 278561, 4, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 262, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (269.67, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (345.91, 166.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 300, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (306.29, 139.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1324)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682788 sequences (100000230 bp) in 2.151 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278598, 10, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 237, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (266.56, 130.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (349.49, 166.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1762, 2288, 2288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (710, 3340)
[M::mem_pestat] mean and std.dev: (1975.00, 336.11)
[M::mem_pestat] low and high boundaries for proper pairs: (184, 3866)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 365, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289)
[M::mem_pestat] mean and std.dev: (377.95, 182.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1639)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277929, 6, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 247, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (265.50, 143.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1343)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (352.81, 167.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (296, 361, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 620)
[M::mem_pestat] mean and std.dev: (334.48, 100.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684030 reads in 308.708 CPU sec, 10.173 real sec
[M::mem_process_seqs] Processed 683444 reads in 243.201 CPU sec, 8.149 real sec
[M::mem_process_seqs] Processed 682610 reads in 271.810 CPU sec, 9.135 real sec
sorting block 1, #reads = 1070945 mem = 536878702 peak = 696258560
[M::process] read 682144 sequences (100000212 bp) in 2.316 CPU sec, 2.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278003, 3, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 227, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (228.94, 93.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 342, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (355.73, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 308, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (298.42, 132.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 2, #reads = 1070586 mem = 536880166 peak = 865124352
[M::process] read 681382 sequences (100000217 bp) in 2.542 CPU sec, 2.911 real sec...
[M::process] read 681094 sequences (100000247 bp) in 2.038 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277574, 6, 58)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 268, 783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2009)
[M::mem_pestat] mean and std.dev: (360.72, 314.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2622)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 343, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (355.80, 166.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 337, 635)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1385)
[M::mem_pestat] mean and std.dev: (365.56, 235.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1760)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682788 reads in 236.353 CPU sec, 8.331 real sec
[M::mem_process_seqs] Processed 682144 reads in 162.742 CPU sec, 5.809 real sec
sorting block 3, #reads = 1069484 mem = 536879118 peak = 865124352
[M::process] read 681308 sequences (100000112 bp) in 2.490 CPU sec, 2.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 277436, 10, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 165, 252)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 496)
[M::mem_pestat] mean and std.dev: (191.73, 94.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 618)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (355.36, 164.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (485, 1016, 1714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4172)
[M::mem_pestat] mean and std.dev: (986.33, 570.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5401)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 204, 625)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513)
[M::mem_pestat] mean and std.dev: (358.46, 326.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1957)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 680906 sequences (100000274 bp) in 2.065 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 277584, 6, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 247, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (257.67, 110.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (355.96, 165.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 234, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158)
[M::mem_pestat] mean and std.dev: (306.97, 191.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1480)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681382 reads in 240.401 CPU sec, 8.643 real sec
[M::mem_process_seqs] Processed 681094 reads in 163.103 CPU sec, 5.823 real sec
sorting block 4, #reads = 1068575 mem = 536878602 peak = 865124352
[M::process] read 681048 sequences (100000159 bp) in 2.495 CPU sec, 2.962 real sec...
[M::process] read 686180 sequences (100000206 bp) in 2.101 CPU sec, 2.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277644, 5, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 226, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (245.48, 136.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (360.94, 170.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 300, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (298.08, 161.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681048 reads in 137.119 CPU sec, 5.035 real sec
sorting block 5, #reads = 1066880 mem = 536878030 peak = 865124352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 277259, 3, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 377, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363)
[M::mem_pestat] mean and std.dev: (369.16, 223.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1752)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (350.11, 161.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 278, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (286.31, 104.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 746)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 277587, 16, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 256, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1234)
[M::mem_pestat] mean and std.dev: (308.91, 215.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1594)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (356.40, 165.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (454, 1426, 1426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3370)
[M::mem_pestat] mean and std.dev: (896.38, 578.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4342)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 380, 1007)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527)
[M::mem_pestat] mean and std.dev: (526.62, 386.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3287)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684804 sequences (100000049 bp) in 2.248 CPU sec, 2.381 real sec...
[M::mem_process_seqs] Processed 681308 reads in 377.453 CPU sec, 13.794 real sec
[M::mem_process_seqs] Processed 680906 reads in 299.535 CPU sec, 11.015 real sec
sorting block 6, #reads = 1065979 mem = 536878880 peak = 865124352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278798, 4, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 270, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083)
[M::mem_pestat] mean and std.dev: (278.40, 163.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1391)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 317, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (336.60, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 292, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (279.33, 116.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683966 sequences (100000245 bp) in 2.882 CPU sec, 3.444 real sec...
[M::process] read 683250 sequences (100000090 bp) in 2.057 CPU sec, 2.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278672, 12, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 319, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (270.94, 143.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (341.93, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 184, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (270.75, 181.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 242, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (275.94, 120.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686180 reads in 242.681 CPU sec, 9.032 real sec
sorting block 7, #reads = 1068324 mem = 536878592 peak = 865124352
[M::mem_process_seqs] Processed 684804 reads in 160.451 CPU sec, 5.899 real sec
[M::process] read 683010 sequences (100000188 bp) in 2.581 CPU sec, 3.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 278406, 3, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 340, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1876)
[M::mem_pestat] mean and std.dev: (530.68, 502.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2541)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (346.16, 166.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 225, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (271.27, 160.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682320 sequences (100000149 bp) in 2.155 CPU sec, 2.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278248, 9, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 336, 800)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012)
[M::mem_pestat] mean and std.dev: (498.65, 440.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2618)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (349.27, 167.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 239, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (256.12, 103.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683966 reads in 238.969 CPU sec, 9.128 real sec
sorting block 8, #reads = 1071534 mem = 536878652 peak = 888344576
[M::mem_process_seqs] Processed 683250 reads in 158.733 CPU sec, 6.002 real sec
sorting block 9, #reads = 1072075 mem = 536878065 peak = 921608192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277850, 15, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 239, 620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486)
[M::mem_pestat] mean and std.dev: (409.68, 316.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1919)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (349.44, 165.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 25, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161)
[M::mem_pestat] mean and std.dev: (159.54, 207.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1543)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 208, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (267.20, 128.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682358 sequences (100000222 bp) in 2.763 CPU sec, 3.170 real sec...
[M::process] read 681868 sequences (100000116 bp) in 2.162 CPU sec, 2.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 277969, 13, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 308, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (320.76, 170.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (353.39, 167.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (635, 1240, 1240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2450)
[M::mem_pestat] mean and std.dev: (931.08, 467.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3055)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 335, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (303.95, 127.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1343)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683010 reads in 230.584 CPU sec, 8.278 real sec
[M::mem_process_seqs] Processed 682320 reads in 157.522 CPU sec, 5.621 real sec
sorting block 10, #reads = 1070945 mem = 536880567 peak = 921608192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278414, 2, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 280, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (306.29, 185.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1401)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (355.08, 168.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 284, 630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1462)
[M::mem_pestat] mean and std.dev: (339.95, 194.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1878)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681790 sequences (100000289 bp) in 3.018 CPU sec, 3.491 real sec...
[M::process] read 681836 sequences (100000133 bp) in 2.137 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 277532, 2, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 328, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (293.17, 148.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (354.68, 166.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 258, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211)
[M::mem_pestat] mean and std.dev: (337.89, 190.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1553)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682358 reads in 230.789 CPU sec, 8.208 real sec
[M::mem_process_seqs] Processed 681868 reads in 157.678 CPU sec, 5.628 real sec
sorting block 11, #reads = 1070530 mem = 536881323 peak = 921608192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277362, 3, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 217, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (225.70, 123.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (353.30, 166.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 297, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (290.50, 127.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681486 sequences (100000180 bp) in 2.998 CPU sec, 3.793 real sec...
[M::process] read 681050 sequences (100000039 bp) in 2.364 CPU sec, 2.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278007, 5, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 232, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (269.33, 171.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1359)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (351.95, 166.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 308, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109)
[M::mem_pestat] mean and std.dev: (354.37, 233.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1418)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681790 reads in 232.508 CPU sec, 8.111 real sec
sorting block 12, #reads = 1069307 mem = 536877827 peak = 921608192
[M::mem_process_seqs] Processed 681836 reads in 157.870 CPU sec, 5.363 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 277817, 10, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (302.29, 175.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 341, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (355.39, 167.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 829, 5266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15388)
[M::mem_pestat] mean and std.dev: (1912.10, 2212.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20449)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 308, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (346.76, 189.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1383)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685890 sequences (100000162 bp) in 3.086 CPU sec, 3.751 real sec...
sorting block 13, #reads = 1068831 mem = 536877857 peak = 921608192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278056, 7, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (249, 263, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (317.75, 127.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 347, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (361.94, 171.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 292, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213)
[M::mem_pestat] mean and std.dev: (341.68, 232.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1547)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684518 sequences (100000058 bp) in 2.130 CPU sec, 2.173 real sec...
[M::mem_process_seqs] Processed 681486 reads in 226.838 CPU sec, 8.073 real sec
[M::mem_process_seqs] Processed 681050 reads in 155.623 CPU sec, 5.577 real sec
sorting block 14, #reads = 1067948 mem = 536880906 peak = 921608192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279181, 7, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 301, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (318.62, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 316, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (336.32, 165.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 312, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301)
[M::mem_pestat] mean and std.dev: (391.62, 264.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1674)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683234 sequences (100000192 bp) in 2.871 CPU sec, 3.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278877, 8, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 278, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185)
[M::mem_pestat] mean and std.dev: (347.43, 234.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1515)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (343.74, 166.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 281, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (296.35, 130.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682684 sequences (100000137 bp) in 2.250 CPU sec, 2.314 real sec...
[M::mem_process_seqs] Processed 685890 reads in 225.401 CPU sec, 7.697 real sec
[M::mem_process_seqs] Processed 684518 reads in 154.115 CPU sec, 5.238 real sec
sorting block 15, #reads = 1069125 mem = 536878467 peak = 921608192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278774, 11, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 310, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (281.78, 156.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (351.44, 169.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (293, 2322, 8508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24938)
[M::mem_pestat] mean and std.dev: (3210.73, 3376.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 33153)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 262, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (282.70, 101.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682170 sequences (100000065 bp) in 3.003 CPU sec, 3.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279101, 4, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 247, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (254.03, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 341, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (356.91, 172.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 327, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271)
[M::mem_pestat] mean and std.dev: (351.69, 226.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1639)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682628 sequences (100000158 bp) in 2.168 CPU sec, 2.174 real sec...
[M::mem_process_seqs] Processed 683234 reads in 216.939 CPU sec, 7.211 real sec
sorting block 16, #reads = 1070820 mem = 536876500 peak = 921608192
[M::mem_process_seqs] Processed 682684 reads in 151.375 CPU sec, 4.987 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 277831, 6, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 300, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1173)
[M::mem_pestat] mean and std.dev: (309.63, 171.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1506)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 344, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (359.20, 172.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 243, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (304.63, 190.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681486 sequences (100000190 bp) in 2.940 CPU sec, 3.187 real sec...
[M::process] read 681234 sequences (100000125 bp) in 2.144 CPU sec, 2.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278285, 11, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 269, 619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551)
[M::mem_pestat] mean and std.dev: (431.70, 358.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2017)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 340, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (356.78, 172.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (544, 749, 2444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6244)
[M::mem_pestat] mean and std.dev: (889.67, 716.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8144)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 221, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (293.78, 159.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682170 reads in 217.232 CPU sec, 7.079 real sec
sorting block 17, #reads = 1071331 mem = 536882674 peak = 921608192
[M::mem_process_seqs] Processed 682628 reads in 150.723 CPU sec, 4.863 real sec
sorting block 18, #reads = 1070325 mem = 536883253 peak = 979099648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 277925, 4, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 312, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192)
[M::mem_pestat] mean and std.dev: (344.82, 193.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1511)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 346, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (360.24, 171.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 239, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (293.03, 165.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681618 sequences (100000279 bp) in 3.062 CPU sec, 3.204 real sec...
[M::process] read 681802 sequences (100000057 bp) in 2.121 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278082, 4, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 248, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (310.26, 215.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (359.95, 170.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 355, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (304.88, 119.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681486 reads in 213.046 CPU sec, 6.795 real sec
[M::mem_process_seqs] Processed 681234 reads in 145.482 CPU sec, 4.626 real sec
sorting block 19, #reads = 1069589 mem = 536883642 peak = 979099648
[M::process] read 681388 sequences (100000185 bp) in 2.751 CPU sec, 2.836 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 277972, 13, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 219, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (261.94, 140.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (356.53, 169.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (364, 462, 1697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4363)
[M::mem_pestat] mean and std.dev: (769.15, 626.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5696)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 463, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1627)
[M::mem_pestat] mean and std.dev: (458.08, 273.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2091)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681022 sequences (100000046 bp) in 2.100 CPU sec, 2.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278523, 10, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 208, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (265.14, 177.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1399)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 340, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (356.76, 170.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1092, 2216, 2721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5979)
[M::mem_pestat] mean and std.dev: (1877.33, 858.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7608)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 275, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (299.00, 136.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681618 reads in 197.978 CPU sec, 6.267 real sec
sorting block 20, #reads = 1068860 mem = 536878401 peak = 979099648
[M::mem_process_seqs] Processed 681802 reads in 133.369 CPU sec, 4.241 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278086, 4, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 281, 576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290)
[M::mem_pestat] mean and std.dev: (303.26, 185.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1647)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 344, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (358.67, 169.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 310, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (321.78, 143.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686148 sequences (100000170 bp) in 2.946 CPU sec, 3.012 real sec...
[M::process] read 683936 sequences (100000088 bp) in 2.280 CPU sec, 2.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 278240, 6, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 350, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165)
[M::mem_pestat] mean and std.dev: (344.91, 179.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1478)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 349, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (363.85, 173.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 306, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (339.06, 172.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681388 reads in 196.759 CPU sec, 6.215 real sec
sorting block 21, #reads = 1068788 mem = 536879418 peak = 979099648
[M::mem_process_seqs] Processed 681022 reads in 222.535 CPU sec, 7.048 real sec
sorting block 22, #reads = 1067455 mem = 536878167 peak = 979099648
[M::process] read 683142 sequences (100000089 bp) in 2.284 CPU sec, 2.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 279510, 7, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 273, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (294.09, 167.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 316, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (338.32, 169.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 236, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (248.03, 139.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682258 sequences (100000150 bp) in 2.040 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278885, 8, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 249, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (211.97, 108.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 333, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (351.20, 172.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 264, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (297.03, 131.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686148 reads in 187.404 CPU sec, 5.934 real sec
[M::mem_process_seqs] Processed 683936 reads in 210.947 CPU sec, 6.700 real sec
sorting block 23, #reads = 1070481 mem = 536879503 peak = 979099648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278716, 5, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 274, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (292.49, 141.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 338, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (355.32, 172.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 378, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (334.70, 117.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 866)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681916 sequences (100000060 bp) in 2.659 CPU sec, 2.730 real sec...
[M::process] read 682372 sequences (100000106 bp) in 2.150 CPU sec, 2.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 277905, 3, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 421, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142)
[M::mem_pestat] mean and std.dev: (386.15, 209.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1452)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 345, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (360.83, 173.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 319, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (327.14, 197.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1509)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683142 reads in 182.862 CPU sec, 5.776 real sec
sorting block 24, #reads = 1071883 mem = 536879781 peak = 979099648
[M::mem_process_seqs] Processed 682258 reads in 122.009 CPU sec, 3.874 real sec
[M::process] read 681392 sequences (100000035 bp) in 2.363 CPU sec, 2.400 real sec...
[M::process] read 681140 sequences (100000108 bp) in 2.100 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278414, 11, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 261, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (266.31, 140.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 341, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (358.05, 172.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 525, 848)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2170)
[M::mem_pestat] mean and std.dev: (463.18, 338.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2831)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 295, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (254.83, 117.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682372 reads in 101.967 CPU sec, 3.221 real sec
sorting block 25, #reads = 1069477 mem = 536880141 peak = 979099648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277999, 4, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 237, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (250.90, 154.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 347, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (362.21, 174.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 275, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (298.79, 127.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278223, 2, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 279, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (281.33, 168.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 347, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (362.08, 171.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 289, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262)
[M::mem_pestat] mean and std.dev: (289.50, 169.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1620)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681364 sequences (100000270 bp) in 1.808 CPU sec, 1.810 real sec...
[M::mem_process_seqs] Processed 681916 reads in 267.267 CPU sec, 8.451 real sec
[M::mem_process_seqs] Processed 681392 reads in 135.174 CPU sec, 4.310 real sec
sorting block 26, #reads = 1070407 mem = 536878769 peak = 979099648
[M::process] read 681378 sequences (100000113 bp) in 2.137 CPU sec, 2.210 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 277914, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 239, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (252.18, 139.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 341, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (355.66, 166.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 354, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194)
[M::mem_pestat] mean and std.dev: (322.30, 182.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1540)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680906 sequences (100000181 bp) in 1.550 CPU sec, 1.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 278242, 6, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 235, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (240.75, 90.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (355.25, 167.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 300, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (289.73, 106.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 879)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681140 reads in 169.116 CPU sec, 5.371 real sec
sorting block 27, #reads = 1069328 mem = 536880062 peak = 979099648
[M::mem_process_seqs] Processed 681364 reads in 111.491 CPU sec, 3.545 real sec
[M::process] read 680730 sequences (100000152 bp) in 2.265 CPU sec, 2.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278175, 4, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 281, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089)
[M::mem_pestat] mean and std.dev: (279.38, 139.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1402)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 342, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (356.92, 168.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 271, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (246.79, 105.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686378 sequences (100000011 bp) in 1.750 CPU sec, 1.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 277708, 3, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 261, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108)
[M::mem_pestat] mean and std.dev: (289.32, 192.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 345, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (360.05, 169.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 347, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (358.52, 173.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1366)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681378 reads in 161.976 CPU sec, 5.123 real sec
sorting block 28, #reads = 1068599 mem = 536878536 peak = 979099648
[M::mem_process_seqs] Processed 680906 reads in 182.210 CPU sec, 5.784 real sec
sorting block 29, #reads = 1069023 mem = 536878512 peak = 979099648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278209, 4, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 336, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1285)
[M::mem_pestat] mean and std.dev: (331.10, 224.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1650)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (364.32, 172.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 258, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (252.96, 96.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687518 sequences (100000021 bp) in 2.418 CPU sec, 2.469 real sec...
[M::process] read 685534 sequences (100000200 bp) in 2.200 CPU sec, 2.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 279659, 3, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 228, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (228.13, 85.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 656)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 315, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (337.47, 168.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 352, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073)
[M::mem_pestat] mean and std.dev: (377.93, 155.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1346)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680730 reads in 177.641 CPU sec, 5.614 real sec
[M::mem_process_seqs] Processed 686378 reads in 125.116 CPU sec, 3.963 real sec
sorting block 30, #reads = 1067903 mem = 536882187 peak = 979099648
[M::process] read 684618 sequences (100000174 bp) in 2.411 CPU sec, 2.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 280018, 8, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 337, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218)
[M::mem_pestat] mean and std.dev: (329.07, 161.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1574)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 323, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (340.87, 166.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 370, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1255)
[M::mem_pestat] mean and std.dev: (341.98, 161.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1604)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684372 sequences (100000142 bp) in 1.940 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 280584, 3, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 237, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (241.58, 123.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 311, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (332.75, 165.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 309, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127)
[M::mem_pestat] mean and std.dev: (341.69, 154.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1432)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685534 reads in 180.605 CPU sec, 5.714 real sec
sorting block 31, #reads = 1068537 mem = 536880215 peak = 979099648
[M::mem_process_seqs] Processed 687518 reads in 202.310 CPU sec, 6.420 real sec
[M::process] read 684244 sequences (100000293 bp) in 2.203 CPU sec, 2.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279518, 6, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 245, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (294.82, 190.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1344)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 330, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (346.73, 168.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 352, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (361.33, 152.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684128 sequences (100000212 bp) in 1.678 CPU sec, 1.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278388, 7, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (254.03, 149.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 333, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (348.18, 167.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 259, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (297.18, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279248, 3, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 242, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (248.78, 117.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (346.97, 166.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 363, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (317.50, 153.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684618 reads in 254.621 CPU sec, 8.116 real sec
sorting block 32, #reads = 1069595 mem = 536882015 peak = 979099648
[M::mem_process_seqs] Processed 684244 reads in 194.415 CPU sec, 6.227 real sec
[M::mem_process_seqs] Processed 684372 reads in 216.848 CPU sec, 6.950 real sec
sorting block 33, #reads = 1067150 mem = 536877919 peak = 979099648
[M::process] read 683610 sequences (100000002 bp) in 2.677 CPU sec, 2.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 279197, 9, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 190, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (202.66, 101.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 332, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (348.02, 167.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 316, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (312.84, 151.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683144 sequences (100000024 bp) in 1.946 CPU sec, 1.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278911, 7, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 303, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105)
[M::mem_pestat] mean and std.dev: (318.79, 162.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1415)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 334, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (349.09, 167.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 220, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (230.41, 73.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 697)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682494 sequences (100000114 bp) in 1.610 CPU sec, 1.604 real sec...
[M::mem_process_seqs] Processed 684128 reads in 182.475 CPU sec, 5.781 real sec
sorting block 34, #reads = 1068107 mem = 536879401 peak = 979099648
[M::mem_process_seqs] Processed 683610 reads in 122.697 CPU sec, 3.878 real sec
[M::process] read 681936 sequences (100000097 bp) in 2.174 CPU sec, 2.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278746, 11, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 277, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (287.18, 161.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (348.04, 164.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (627, 1408, 9840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 28266)
[M::mem_pestat] mean and std.dev: (3509.64, 3939.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 37479)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 235, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (232.04, 77.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 714)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685436 sequences (100000077 bp) in 1.938 CPU sec, 1.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 278108, 3, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 220, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (230.77, 105.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 337, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (350.70, 164.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 362, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (333.03, 145.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 278381, 6, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 258, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (304.13, 177.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (355.30, 166.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 260, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (254.20, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683144 reads in 250.763 CPU sec, 7.982 real sec
sorting block 35, #reads = 1066779 mem = 536879558 peak = 979099648
[M::mem_process_seqs] Processed 682494 reads in 191.741 CPU sec, 6.128 real sec
[M::mem_process_seqs] Processed 681936 reads in 213.146 CPU sec, 6.813 real sec
sorting block 36, #reads = 1065752 mem = 536879281 peak = 1023762432
[M::process] read 693080 sequences (100000168 bp) in 2.588 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 279749, 8, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 327, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117)
[M::mem_pestat] mean and std.dev: (327.53, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1427)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 319, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (339.71, 167.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 265, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (282.25, 123.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689804 sequences (100000117 bp) in 1.835 CPU sec, 1.858 real sec...
[M::process] read 687622 sequences (100000127 bp) in 1.630 CPU sec, 1.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282609, 3, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 194, 607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559)
[M::mem_pestat] mean and std.dev: (354.34, 303.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2035)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 278, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (302.38, 151.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 225, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (231.51, 93.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 736)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685436 reads in 196.068 CPU sec, 6.209 real sec
sorting block 37, #reads = 1064952 mem = 536880072 peak = 1023762432
[M::mem_process_seqs] Processed 693080 reads in 133.401 CPU sec, 4.221 real sec
sorting block 38, #reads = 1061266 mem = 536881685 peak = 1023762432
[M::process] read 686102 sequences (100000274 bp) in 2.406 CPU sec, 2.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 281478, 7, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 291, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (329.24, 158.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1343)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 298, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (317.48, 156.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 357, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (340.85, 136.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1316)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685434 sequences (100000122 bp) in 1.740 CPU sec, 1.741 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 280779, 3, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 207, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (276.04, 161.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 309, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (327.37, 158.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (246.18, 113.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689804 reads in 197.628 CPU sec, 6.267 real sec
[M::mem_process_seqs] Processed 687622 reads in 221.598 CPU sec, 7.048 real sec
sorting block 39, #reads = 1062125 mem = 536878883 peak = 1023762432
[M::process] read 684506 sequences (100000276 bp) in 2.038 CPU sec, 2.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 281033, 16, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 280, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (263.49, 102.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (334.17, 161.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (940, 3708, 4756)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12388)
[M::mem_pestat] mean and std.dev: (2907.94, 1736.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16204)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 281, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (302.15, 164.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683512 sequences (100000032 bp) in 1.695 CPU sec, 1.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279947, 8, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 245, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (255.59, 110.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 320, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (336.90, 162.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 407, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111)
[M::mem_pestat] mean and std.dev: (359.86, 149.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1402)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686102 reads in 182.212 CPU sec, 5.957 real sec
sorting block 40, #reads = 1064215 mem = 536879228 peak = 1023762432
[M::mem_process_seqs] Processed 685434 reads in 203.644 CPU sec, 6.575 real sec
[M::process] read 682700 sequences (100000258 bp) in 2.596 CPU sec, 2.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279442, 2, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 274, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (249.43, 124.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 328, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (344.56, 166.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (253.35, 112.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682174 sequences (100000230 bp) in 1.744 CPU sec, 1.757 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279144, 8, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 263, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (267.44, 148.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 336, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (353.25, 171.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 304, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (311.41, 141.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278660, 5, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 416, 569)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395)
[M::mem_pestat] mean and std.dev: (413.19, 282.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1808)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 338, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (355.01, 171.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 285, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (272.43, 129.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684506 reads in 287.655 CPU sec, 9.262 real sec
sorting block 41, #reads = 1065455 mem = 536880539 peak = 1023762432
[M::mem_process_seqs] Processed 683512 reads in 217.150 CPU sec, 6.960 real sec
[M::mem_process_seqs] Processed 682700 reads in 242.441 CPU sec, 7.773 real sec
sorting block 42, #reads = 1064712 mem = 536877701 peak = 1023762432
[M::process] read 682992 sequences (100000062 bp) in 2.369 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 278782, 10, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 292, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122)
[M::mem_pestat] mean and std.dev: (313.61, 140.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1427)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 343, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (360.86, 174.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (397, 942, 985)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2161)
[M::mem_pestat] mean and std.dev: (710.50, 381.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2749)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 377, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (312.16, 123.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 687846 sequences (100000165 bp) in 1.890 CPU sec, 1.923 real sec...
[M::process] read 685640 sequences (100000278 bp) in 1.590 CPU sec, 1.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 278738, 11, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 249, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (278.63, 152.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 340, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (359.47, 177.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 443, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122)
[M::mem_pestat] mean and std.dev: (291.80, 180.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1442)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 283, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (280.29, 149.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682174 reads in 201.360 CPU sec, 6.391 real sec
sorting block 43, #reads = 1065718 mem = 536881581 peak = 1023762432
[M::mem_process_seqs] Processed 682992 reads in 139.413 CPU sec, 4.424 real sec
sorting block 44, #reads = 1061942 mem = 536876966 peak = 1023762432
[M::process] read 684480 sequences (100000149 bp) in 2.150 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 280552, 3, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 262, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (250.16, 152.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 928)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 308, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (329.94, 164.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 316, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (315.82, 154.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683832 sequences (100000181 bp) in 1.620 CPU sec, 1.624 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280695, 7, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 253, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (263.45, 114.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 321, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (340.47, 167.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 370, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1259)
[M::mem_pestat] mean and std.dev: (350.24, 204.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1615)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687846 reads in 192.459 CPU sec, 6.154 real sec
[M::process] read 683676 sequences (100000083 bp) in 1.677 CPU sec, 1.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279668, 12, 63)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (239.89, 84.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (343.61, 166.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (835, 1312, 1312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2266)
[M::mem_pestat] mean and std.dev: (1090.64, 217.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2743)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 282, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (281.65, 153.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684480 reads in 98.850 CPU sec, 3.297 real sec
sorting block 45, #reads = 1068790 mem = 536885579 peak = 1023762432
[M::mem_process_seqs] Processed 685640 reads in 336.855 CPU sec, 10.923 real sec
[M::process] read 684170 sequences (100000193 bp) in 2.342 CPU sec, 2.548 real sec...
[M::process] read 682900 sequences (100000164 bp) in 1.630 CPU sec, 1.624 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279514, 6, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 291, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (301.41, 150.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (348.57, 168.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 231, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (256.36, 119.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683676 reads in 99.563 CPU sec, 3.176 real sec
sorting block 46, #reads = 1069335 mem = 536878133 peak = 1023762432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 279463, 6, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 212, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473)
[M::mem_pestat] mean and std.dev: (203.19, 71.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 578)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (348.52, 168.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 329, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (313.90, 143.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682576 sequences (100000042 bp) in 1.728 CPU sec, 1.729 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 279632, 1, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 300, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (278.11, 126.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 331, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (348.29, 169.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 242, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (232.00, 90.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683832 reads in 306.539 CPU sec, 9.808 real sec
sorting block 47, #reads = 1069514 mem = 536878075 peak = 1057050624
[M::mem_process_seqs] Processed 684170 reads in 144.776 CPU sec, 4.665 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 278740, 7, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 229, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488)
[M::mem_pestat] mean and std.dev: (202.76, 80.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 609)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 337, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (353.49, 170.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 350, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (337.25, 159.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1382)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682750 sequences (100000064 bp) in 2.676 CPU sec, 2.739 real sec...
[M::process] read 682340 sequences (100000195 bp) in 2.210 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 279401, 5, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 230, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (304.75, 226.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 340, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (357.31, 172.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 302, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (322.34, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682900 reads in 187.770 CPU sec, 5.927 real sec
sorting block 48, #reads = 1069354 mem = 536881433 peak = 1057050624
[M::mem_process_seqs] Processed 682576 reads in 124.665 CPU sec, 4.032 real sec
sorting block 49, #reads = 1068389 mem = 536882317 peak = 1057050624
[M::process] read 682032 sequences (100000289 bp) in 2.155 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278545, 4, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 346, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (267.60, 115.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 337, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (355.29, 172.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 270, 695)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625)
[M::mem_pestat] mean and std.dev: (380.21, 316.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2090)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689860 sequences (100000212 bp) in 1.740 CPU sec, 1.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278912, 7, 48)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 319, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (304.80, 160.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 343, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (360.58, 174.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 330, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (300.83, 142.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 278559, 9, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 294, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (263.50, 119.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 340, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (357.60, 172.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 301, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (346.31, 185.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682750 reads in 276.366 CPU sec, 8.902 real sec
[M::mem_process_seqs] Processed 682032 reads in 211.922 CPU sec, 6.792 real sec
[M::mem_process_seqs] Processed 682340 reads in 236.334 CPU sec, 7.583 real sec
sorting block 50, #reads = 1064413 mem = 536889620 peak = 1057050624
[M::process] read 689726 sequences (100000058 bp) in 2.398 CPU sec, 2.494 real sec...
sorting block 51, #reads = 1063641 mem = 536878631 peak = 1057050624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 280564, 4, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 217, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (226.57, 107.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 296, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (321.99, 164.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 295, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132)
[M::mem_pestat] mean and std.dev: (353.59, 202.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1442)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686942 sequences (100000232 bp) in 1.867 CPU sec, 1.897 real sec...
[M::process] read 685316 sequences (100000104 bp) in 1.630 CPU sec, 1.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 281194, 5, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 242, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (288.97, 120.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 299, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (320.73, 159.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 226, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (233.13, 80.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689860 reads in 233.109 CPU sec, 7.414 real sec
[M::mem_process_seqs] Processed 689726 reads in 153.833 CPU sec, 4.904 real sec
sorting block 52, #reads = 1056876 mem = 536877479 peak = 1057050624
[M::process] read 684608 sequences (100000151 bp) in 2.753 CPU sec, 2.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 280518, 2, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 256, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (288.79, 162.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 315, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (333.22, 162.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 266, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084)
[M::mem_pestat] mean and std.dev: (333.54, 174.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1380)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683338 sequences (100000199 bp) in 1.650 CPU sec, 1.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 280210, 9, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 291, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150)
[M::mem_pestat] mean and std.dev: (361.61, 190.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1464)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (341.59, 164.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 324, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (327.65, 132.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686942 reads in 206.421 CPU sec, 6.544 real sec
[M::mem_process_seqs] Processed 685316 reads in 232.070 CPU sec, 7.376 real sec
sorting block 53, #reads = 1061667 mem = 536879115 peak = 1057050624
[M::process] read 682562 sequences (100000020 bp) in 2.233 CPU sec, 2.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279897, 10, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 229, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (288.69, 166.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 329, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (345.72, 167.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (498, 498, 969)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1911)
[M::mem_pestat] mean and std.dev: (576.56, 301.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2382)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (289, 352, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (318.39, 106.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682174 sequences (100000033 bp) in 1.810 CPU sec, 1.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279033, 9, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 389, 576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1390)
[M::mem_pestat] mean and std.dev: (379.25, 248.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1797)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 338, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (352.94, 169.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 290, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (313.62, 176.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1397)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 278349, 6, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 297, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319)
[M::mem_pestat] mean and std.dev: (297.30, 185.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1706)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 336, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (352.31, 168.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 284, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (279.10, 136.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684608 reads in 282.905 CPU sec, 9.029 real sec
sorting block 54, #reads = 1062188 mem = 536877062 peak = 1057050624
[M::mem_process_seqs] Processed 683338 reads in 213.776 CPU sec, 6.854 real sec
[M::mem_process_seqs] Processed 682562 reads in 238.977 CPU sec, 7.659 real sec
sorting block 55, #reads = 1062942 mem = 536886291 peak = 1057050624
[M::process] read 681850 sequences (100000092 bp) in 2.475 CPU sec, 2.586 real sec...
[M::process] read 681806 sequences (100000155 bp) in 1.694 CPU sec, 1.723 real sec...
[M::process] read 681270 sequences (100000299 bp) in 1.560 CPU sec, 1.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278506, 14, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 246, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494)
[M::mem_pestat] mean and std.dev: (257.63, 115.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 719)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 340, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (356.54, 170.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (631, 1082, 1815)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4183)
[M::mem_pestat] mean and std.dev: (1116.64, 716.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5367)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 310, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (299.89, 83.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681850 reads in 103.183 CPU sec, 3.287 real sec
sorting block 56, #reads = 1063613 mem = 536879756 peak = 1057050624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277779, 1, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 281, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.20, 168.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 337, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (353.47, 168.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 292, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (329.97, 165.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 41168 sequences (6010689 bp) in 0.237 CPU sec, 0.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278395, 5, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 368, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106)
[M::mem_pestat] mean and std.dev: (320.86, 209.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1425)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 344, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (360.62, 173.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 333, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217)
[M::mem_pestat] mean and std.dev: (373.58, 234.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1560)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682174 reads in 290.135 CPU sec, 9.282 real sec
[M::mem_process_seqs] Processed 681806 reads in 150.249 CPU sec, 4.893 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 16694, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 327, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (344.83, 168.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 57, #reads = 1065592 mem = 536882373 peak = 1057050624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 277950, 11, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 250, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (288.30, 162.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 348, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (364.35, 175.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (46, 534, 2180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6448)
[M::mem_pestat] mean and std.dev: (756.10, 856.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8582)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 241, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (276.73, 141.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 41168 reads in 86.854 CPU sec, 2.809 real sec
[M::mem_process_seqs] Processed 681270 reads in 108.716 CPU sec, 3.524 real sec
sorting block 58, #reads = 1066119 mem = 536885325 peak = 1077563392
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 62927370
overall: 70155526144 mem 11925.534 user 321.632 sys 425.850 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 425.885 sec; CPU: 12247.168 sec
sorting block 59, #reads = 312963 mem = 157777029 peak = 1077563392
uniq header count 1
merging 60 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3998269492
output reads: 63298066
bam_mem_sort: 60 calls 32.254 user 0.502 sys 33.496 real
bam_write: 60 calls 26.683 user 1.762 sys 47.547 real
execute: 1 calls 22.612 user 1.262 sys 456.255 real
merge_files: 1 calls 22.567 user 1.247 sys 30.040 real
parse_chunk: 949 calls 55.111 user 12.382 sys 75.660 real
read_chunk: 2564 calls 27.487 user 17.433 sys 356.264 real
sort_block: 1 calls 18.629 user 17.018 sys 344.088 real
write_chunk: 1613 calls 21.963 user 1.047 sys 28.958 real
overall: 2854928384 mem 1135.831 user 44.189 sys 456.276 real
ls: cannot access '*.cram': No such file or directory