cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1817.061 CPU sec, 610.904 real sec
[M::process] read 688466 sequences (100000198 bp) in 0.947 CPU sec, 0.953 real sec...
[M::process] read 686190 sequences (100000005 bp) in 1.200 CPU sec, 1.195 real sec...
[M::process] read 685354 sequences (100000114 bp) in 1.146 CPU sec, 1.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284639, 3, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 275, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (278.71, 163.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 290, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (311.40, 155.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 242, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070)
[M::mem_pestat] mean and std.dev: (284.36, 138.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1358)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684290 sequences (100000089 bp) in 1.125 CPU sec, 1.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284036, 2, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 222, 1068)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2860)
[M::mem_pestat] mean and std.dev: (420.06, 466.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3756)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 305, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (322.44, 158.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 398, 778)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1984)
[M::mem_pestat] mean and std.dev: (447.88, 327.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2587)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283639, 5, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 250, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (297.90, 136.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 310, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (326.95, 159.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 342, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (335.09, 139.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688466 reads in 215.236 CPU sec, 6.904 real sec
[M::mem_process_seqs] Processed 686190 reads in 146.995 CPU sec, 4.773 real sec
[M::mem_process_seqs] Processed 685354 reads in 158.678 CPU sec, 5.165 real sec
sorting block 0, #reads = 1083057 mem = 536882696 peak = 588881920
[M::process] read 683834 sequences (100000235 bp) in 1.774 CPU sec, 1.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 283454, 4, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 370, 847)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2121)
[M::mem_pestat] mean and std.dev: (460.72, 288.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2758)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (332.53, 161.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 258, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (287.57, 136.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683346 sequences (100000117 bp) in 1.225 CPU sec, 1.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283140, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 311, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (254.19, 107.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682472 sequences (100000037 bp) in 1.110 CPU sec, 1.109 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (336.43, 162.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 206, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458)
[M::mem_pestat] mean and std.dev: (205.94, 45.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 550)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684290 reads in 140.434 CPU sec, 4.469 real sec
sorting block 1, #reads = 1081151 mem = 536878555 peak = 775274496
[M::mem_process_seqs] Processed 683834 reads in 87.195 CPU sec, 2.763 real sec
sorting block 2, #reads = 1080532 mem = 536877613 peak = 860483584
[M::process] read 681912 sequences (100000041 bp) in 1.650 CPU sec, 1.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282207, 9, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 463, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (412.19, 199.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (338.43, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 265, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (29, 564)
[M::mem_pestat] mean and std.dev: (277.60, 82.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 671)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682006 sequences (100000112 bp) in 1.270 CPU sec, 1.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282256, 7, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 213, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155)
[M::mem_pestat] mean and std.dev: (348.73, 261.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1483)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (340.18, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 269, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (256.71, 138.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282377, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 320, 786)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038)
[M::mem_pestat] mean and std.dev: (509.88, 461.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2664)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 326, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (338.16, 158.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 350, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (274.11, 108.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683346 reads in 206.407 CPU sec, 6.620 real sec
[M::mem_process_seqs] Processed 682472 reads in 151.992 CPU sec, 4.887 real sec
[M::mem_process_seqs] Processed 681912 reads in 164.078 CPU sec, 5.298 real sec
sorting block 3, #reads = 1080148 mem = 536879764 peak = 860483584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281815, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 225, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (231.38, 77.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 323, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (336.77, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 443, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (350.93, 147.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682134 sequences (100000011 bp) in 2.266 CPU sec, 2.434 real sec...
[M::mem_process_seqs] Processed 682006 reads in 81.260 CPU sec, 2.681 real sec
sorting block 4, #reads = 1078201 mem = 536878718 peak = 860483584
[M::process] read 681766 sequences (100000275 bp) in 2.016 CPU sec, 2.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282691, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 223, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (254.67, 125.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 323, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (336.10, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 311, 2094)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5820)
[M::mem_pestat] mean and std.dev: (1029.75, 1409.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7683)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686170 sequences (100000014 bp) in 1.220 CPU sec, 1.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282033, 3, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 267, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272)
[M::mem_pestat] mean and std.dev: (390.25, 284.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1628)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (342.58, 163.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 342, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637)
[M::mem_pestat] mean and std.dev: (395.95, 266.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2121)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686396 sequences (100000077 bp) in 1.205 CPU sec, 1.208 real sec...
[M::mem_process_seqs] Processed 682134 reads in 144.703 CPU sec, 4.585 real sec
sorting block 5, #reads = 1077205 mem = 536878016 peak = 860483584
[M::process] read 685248 sequences (100000234 bp) in 1.362 CPU sec, 1.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283647, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 256, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531)
[M::mem_pestat] mean and std.dev: (251.08, 85.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 304, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (324.45, 161.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 353, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (275.69, 136.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686170 reads in 82.742 CPU sec, 2.821 real sec
[M::mem_process_seqs] Processed 681766 reads in 189.644 CPU sec, 6.219 real sec
sorting block 6, #reads = 1077948 mem = 536876592 peak = 860483584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283714, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 201, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (268.26, 177.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1322)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 305, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (323.11, 158.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 256, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1770)
[M::mem_pestat] mean and std.dev: (298.36, 163.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2293)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684626 sequences (100000089 bp) in 2.041 CPU sec, 2.170 real sec...
[M::process] read 684100 sequences (100000209 bp) in 1.250 CPU sec, 1.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283381, 6, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 310, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (326.23, 159.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 321, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (305.64, 111.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686396 reads in 153.160 CPU sec, 4.883 real sec
[M::mem_process_seqs] Processed 685248 reads in 87.214 CPU sec, 2.774 real sec
sorting block 7, #reads = 1078262 mem = 536880896 peak = 860483584
[M::process] read 683320 sequences (100000290 bp) in 1.842 CPU sec, 1.971 real sec...
[M::process] read 683378 sequences (100000134 bp) in 1.260 CPU sec, 1.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282767, 10, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 377, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221)
[M::mem_pestat] mean and std.dev: (348.68, 197.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1556)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (331.88, 160.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (585, 585, 4002)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10836)
[M::mem_pestat] mean and std.dev: (1557.40, 1604.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14253)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 344, 736)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1684)
[M::mem_pestat] mean and std.dev: (472.26, 301.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2158)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684100 reads in 76.452 CPU sec, 2.426 real sec
sorting block 8, #reads = 1081656 mem = 536879051 peak = 860483584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283127, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (263, 506, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442)
[M::mem_pestat] mean and std.dev: (534.96, 386.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2081)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (331.74, 161.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 399, 663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469)
[M::mem_pestat] mean and std.dev: (523.62, 347.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1913)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283015, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 348, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (301.19, 172.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 321, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (335.54, 161.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683106 sequences (100000300 bp) in 1.339 CPU sec, 1.342 real sec...
[M::mem_process_seqs] Processed 684626 reads in 217.759 CPU sec, 6.951 real sec
[M::mem_process_seqs] Processed 683320 reads in 101.007 CPU sec, 3.238 real sec
sorting block 9, #reads = 1079818 mem = 536878726 peak = 902664192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282229, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 354, 589)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367)
[M::mem_pestat] mean and std.dev: (413.20, 296.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1756)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (336.58, 162.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 339, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (299.31, 102.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682670 sequences (100000059 bp) in 2.119 CPU sec, 2.238 real sec...
[M::process] read 682500 sequences (100000107 bp) in 1.280 CPU sec, 1.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283081, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 274, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149)
[M::mem_pestat] mean and std.dev: (313.65, 151.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1471)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (336.93, 161.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267)
[M::mem_pestat] mean and std.dev: (304.50, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1622)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683378 reads in 143.518 CPU sec, 4.582 real sec
sorting block 10, #reads = 1081007 mem = 536880767 peak = 902664192
[M::mem_process_seqs] Processed 683106 reads in 88.347 CPU sec, 2.821 real sec
sorting block 11, #reads = 1079691 mem = 536881068 peak = 930754560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282843, 12, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 375, 624)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1422)
[M::mem_pestat] mean and std.dev: (411.03, 238.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1821)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (336.95, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 367, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (278.92, 93.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 280, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (242.75, 66.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681850 sequences (100000215 bp) in 2.030 CPU sec, 2.164 real sec...
[M::process] read 684016 sequences (100000127 bp) in 1.280 CPU sec, 1.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282087, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 537, 658)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1576)
[M::mem_pestat] mean and std.dev: (455.94, 307.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2035)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 320, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (335.34, 160.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 569, 921)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2369)
[M::mem_pestat] mean and std.dev: (471.73, 287.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3093)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682670 reads in 142.772 CPU sec, 4.552 real sec
[M::mem_process_seqs] Processed 682500 reads in 88.783 CPU sec, 2.833 real sec
sorting block 12, #reads = 1078762 mem = 536876014 peak = 930754560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 282567, 8, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 341, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133)
[M::mem_pestat] mean and std.dev: (345.57, 187.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1446)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (340.01, 162.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 296, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1361)
[M::mem_pestat] mean and std.dev: (375.18, 290.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1756)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686898 sequences (100000090 bp) in 2.130 CPU sec, 2.286 real sec...
[M::process] read 684992 sequences (100000049 bp) in 1.320 CPU sec, 1.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283745, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 225, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (265.83, 176.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (333.71, 163.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 316, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (301.15, 110.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681850 reads in 141.147 CPU sec, 4.498 real sec
[M::mem_process_seqs] Processed 684016 reads in 87.535 CPU sec, 2.782 real sec
sorting block 13, #reads = 1078202 mem = 536878460 peak = 930754560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284067, 5, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 322, 562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1404)
[M::mem_pestat] mean and std.dev: (409.40, 325.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1825)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 299, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (319.11, 158.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 264, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566)
[M::mem_pestat] mean and std.dev: (259.80, 94.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683986 sequences (100000043 bp) in 2.172 CPU sec, 2.335 real sec...
[M::process] read 683560 sequences (100000041 bp) in 1.320 CPU sec, 1.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283469, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (297.00, 128.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 312, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (328.40, 160.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 370, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (351.25, 126.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686898 reads in 139.855 CPU sec, 4.457 real sec
[M::mem_process_seqs] Processed 684992 reads in 87.087 CPU sec, 2.768 real sec
sorting block 14, #reads = 1080042 mem = 536877797 peak = 930754560
[M::process] read 683538 sequences (100000222 bp) in 1.986 CPU sec, 2.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282883, 6, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 282, 1488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3986)
[M::mem_pestat] mean and std.dev: (702.09, 599.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5235)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (339.50, 165.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 374, 1005)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2465)
[M::mem_pestat] mean and std.dev: (602.09, 538.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3195)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682784 sequences (100000132 bp) in 1.340 CPU sec, 1.345 real sec...
[M::mem_process_seqs] Processed 683560 reads in 81.676 CPU sec, 2.591 real sec
sorting block 15, #reads = 1081733 mem = 536881338 peak = 930754560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283417, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 280, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (216.60, 75.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 319, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (335.20, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 303, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (296.55, 101.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282917, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 248, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209)
[M::mem_pestat] mean and std.dev: (329.46, 189.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1559)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (338.50, 165.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 228, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 497)
[M::mem_pestat] mean and std.dev: (250.53, 72.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 594)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681958 sequences (100000219 bp) in 1.350 CPU sec, 1.353 real sec...
[M::mem_process_seqs] Processed 683986 reads in 215.673 CPU sec, 6.887 real sec
[M::mem_process_seqs] Processed 683538 reads in 100.147 CPU sec, 3.209 real sec
sorting block 16, #reads = 1080170 mem = 536879225 peak = 943456256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282630, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 296, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (312.95, 127.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (341.53, 166.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 249, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729)
[M::mem_pestat] mean and std.dev: (262.19, 100.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682436 sequences (100000154 bp) in 2.421 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282164, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 284, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (313.29, 160.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1320)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (342.51, 164.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 233, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (102, 397)
[M::mem_pestat] mean and std.dev: (222.71, 31.43)
[M::mem_pestat] low and high boundaries for proper pairs: (43, 456)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682496 sequences (100000235 bp) in 1.290 CPU sec, 1.297 real sec...
[M::mem_process_seqs] Processed 682784 reads in 137.668 CPU sec, 4.378 real sec
sorting block 17, #reads = 1079945 mem = 536881013 peak = 943456256
[M::mem_process_seqs] Processed 681958 reads in 86.956 CPU sec, 2.754 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282709, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 291, 1662)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4694)
[M::mem_pestat] mean and std.dev: (1156.47, 1483.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7091)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 324, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (339.66, 163.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 356, 579)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1295)
[M::mem_pestat] mean and std.dev: (442.86, 238.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1653)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1079393 mem = 536878800 peak = 943456256
[M::process] read 681874 sequences (100000009 bp) in 1.973 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283133, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 263, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (276.44, 169.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682358 sequences (100000139 bp) in 1.320 CPU sec, 1.328 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (204, 323, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (339.88, 165.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 262, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (310.38, 146.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682436 reads in 136.681 CPU sec, 4.353 real sec
[M::mem_process_seqs] Processed 682496 reads in 86.422 CPU sec, 2.746 real sec
sorting block 19, #reads = 1077944 mem = 536877553 peak = 943456256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281943, 9, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 357, 891)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2273)
[M::mem_pestat] mean and std.dev: (492.84, 342.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2964)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (342.06, 163.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (288.84, 92.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687738 sequences (100000046 bp) in 2.019 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283060, 10, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 200, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (223.53, 108.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (344.06, 166.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (72, 116, 1760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5136)
[M::mem_pestat] mean and std.dev: (590.00, 766.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6824)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 287, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 597)
[M::mem_pestat] mean and std.dev: (296.54, 84.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 716)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684576 sequences (100000150 bp) in 1.320 CPU sec, 1.320 real sec...
[M::mem_process_seqs] Processed 681874 reads in 137.635 CPU sec, 4.383 real sec
[M::mem_process_seqs] Processed 682358 reads in 88.079 CPU sec, 2.792 real sec
sorting block 20, #reads = 1076552 mem = 536881219 peak = 943456256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283988, 2, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 248, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (288.13, 112.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 294, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (317.57, 161.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 484, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1356)
[M::mem_pestat] mean and std.dev: (441.06, 309.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1763)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683868 sequences (100000158 bp) in 2.156 CPU sec, 2.291 real sec...
[M::process] read 683032 sequences (100000098 bp) in 1.300 CPU sec, 1.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283361, 6, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 264, 854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2218)
[M::mem_pestat] mean and std.dev: (353.67, 305.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2900)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 314, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (333.07, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 384, 763)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1809)
[M::mem_pestat] mean and std.dev: (426.39, 273.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2332)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687738 reads in 134.688 CPU sec, 4.278 real sec
[M::mem_process_seqs] Processed 684576 reads in 84.133 CPU sec, 2.665 real sec
sorting block 21, #reads = 1078881 mem = 536881960 peak = 943456256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283360, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 282, 564)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212)
[M::mem_pestat] mean and std.dev: (333.23, 194.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1536)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (339.14, 166.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 265, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (267.88, 80.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683060 sequences (100000143 bp) in 2.086 CPU sec, 2.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282634, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 212, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (57, 367)
[M::mem_pestat] mean and std.dev: (194.42, 46.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 429)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (343.00, 167.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 270, 614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1404)
[M::mem_pestat] mean and std.dev: (343.33, 171.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1799)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682660 sequences (100000187 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 683868 reads in 133.109 CPU sec, 4.231 real sec
sorting block 22, #reads = 1081429 mem = 536882364 peak = 943456256
[M::mem_process_seqs] Processed 683032 reads in 84.164 CPU sec, 2.668 real sec
sorting block 23, #reads = 1080528 mem = 536879700 peak = 976584704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283035, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 575, 1059)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775)
[M::mem_pestat] mean and std.dev: (720.00, 639.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3633)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (342.99, 167.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (300, 397, 741)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623)
[M::mem_pestat] mean and std.dev: (503.55, 296.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2064)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681886 sequences (100000241 bp) in 2.196 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283115, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (342.37, 166.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682312 sequences (100000106 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 683060 reads in 135.067 CPU sec, 4.291 real sec
[M::mem_process_seqs] Processed 682660 reads in 85.261 CPU sec, 2.697 real sec
sorting block 24, #reads = 1080316 mem = 536881081 peak = 976584704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282933, 1, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 249, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1514)
[M::mem_pestat] mean and std.dev: (378.88, 368.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1965)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (341.66, 163.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 200, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (227.12, 110.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682404 sequences (100000029 bp) in 2.237 CPU sec, 2.335 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282897, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 293, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (285.34, 122.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 681952 sequences (100000093 bp) in 1.330 CPU sec, 1.337 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (206, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (337.77, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 355, 562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1274)
[M::mem_pestat] mean and std.dev: (359.69, 195.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1630)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681886 reads in 136.121 CPU sec, 4.322 real sec
[M::mem_process_seqs] Processed 682312 reads in 85.552 CPU sec, 2.712 real sec
sorting block 25, #reads = 1079850 mem = 536880381 peak = 976584704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282367, 9, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (307, 357, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (369.71, 144.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (338.01, 162.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 492, 660)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498)
[M::mem_pestat] mean and std.dev: (461.60, 290.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1917)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681584 sequences (100000240 bp) in 2.300 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282420, 11, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 218, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083)
[M::mem_pestat] mean and std.dev: (268.65, 144.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1384)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (340.95, 163.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (64, 644, 1866)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5470)
[M::mem_pestat] mean and std.dev: (894.82, 833.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7272)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 1184)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3130)
[M::mem_pestat] mean and std.dev: (668.80, 635.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4103)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 688246 sequences (100000133 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_process_seqs] Processed 682404 reads in 136.574 CPU sec, 4.336 real sec
sorting block 26, #reads = 1077839 mem = 536882824 peak = 976584704
[M::mem_process_seqs] Processed 681952 reads in 86.069 CPU sec, 2.726 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282366, 2, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (365, 492, 2352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6326)
[M::mem_pestat] mean and std.dev: (909.46, 843.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8313)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (346.95, 166.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 354, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (380.12, 222.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1518)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 27, #reads = 1078855 mem = 536877854 peak = 976584704
[M::process] read 688844 sequences (100000231 bp) in 2.402 CPU sec, 2.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284555, 6, 23)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 292, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (316.90, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 311, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (302.00, 158.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RR
[M::process] read 686852 sequences (100000287 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_process_seqs] Processed 681584 reads in 141.961 CPU sec, 4.506 real sec
[M::mem_process_seqs] Processed 688246 reads in 93.086 CPU sec, 2.946 real sec
sorting block 28, #reads = 1076603 mem = 536878383 peak = 976584704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284538, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 252, 601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395)
[M::mem_pestat] mean and std.dev: (352.12, 221.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1792)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 291, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (314.44, 158.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 271, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (329.68, 136.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685822 sequences (100000129 bp) in 2.413 CPU sec, 2.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284717, 3, 23)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 302, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (321.93, 159.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 322, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (322.95, 176.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1379)
[M::mem_pestat] skip orientation RR
[M::process] read 685518 sequences (100000058 bp) in 1.420 CPU sec, 1.427 real sec...
[M::mem_process_seqs] Processed 688844 reads in 145.162 CPU sec, 4.614 real sec
[M::mem_process_seqs] Processed 686852 reads in 90.291 CPU sec, 2.848 real sec
sorting block 29, #reads = 1080046 mem = 536878051 peak = 976584704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284086, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (356, 451, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (409.59, 161.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 310, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (327.38, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 251, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (272.94, 95.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685186 sequences (100000164 bp) in 2.147 CPU sec, 2.245 real sec...
[M::process] read 684854 sequences (100000129 bp) in 1.390 CPU sec, 1.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283662, 3, 24)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 312, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (328.87, 161.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 284, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (291.14, 124.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685822 reads in 142.093 CPU sec, 4.518 real sec
[M::mem_process_seqs] Processed 685518 reads in 89.346 CPU sec, 2.831 real sec
sorting block 30, #reads = 1079733 mem = 536878070 peak = 976584704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283586, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 334, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (250.88, 126.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 313, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (329.52, 160.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 329, 863)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2031)
[M::mem_pestat] mean and std.dev: (516.62, 419.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2615)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684582 sequences (100000045 bp) in 2.374 CPU sec, 2.485 real sec...
[M::process] read 683580 sequences (100000240 bp) in 1.360 CPU sec, 1.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283169, 3, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 209, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (285.08, 151.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1328)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 314, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (331.27, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 227, 705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1751)
[M::mem_pestat] mean and std.dev: (325.05, 214.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2274)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685186 reads in 146.934 CPU sec, 4.671 real sec
sorting block 31, #reads = 1079418 mem = 536884903 peak = 976584704
[M::mem_process_seqs] Processed 684854 reads in 91.901 CPU sec, 2.915 real sec
sorting block 32, #reads = 1076726 mem = 536878317 peak = 979222528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283205, 8, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 397, 677)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663)
[M::mem_pestat] mean and std.dev: (454.08, 318.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2156)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 317, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (331.98, 161.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (284, 405, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196)
[M::mem_pestat] mean and std.dev: (391.67, 173.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1500)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682906 sequences (100000197 bp) in 2.398 CPU sec, 2.543 real sec...
[M::process] read 683640 sequences (100000073 bp) in 1.407 CPU sec, 1.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282866, 15, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 242, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486)
[M::mem_pestat] mean and std.dev: (298.04, 249.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1930)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 318, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (332.11, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 316, 815)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2135)
[M::mem_pestat] mean and std.dev: (430.31, 375.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2795)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (303, 350, 869)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2001)
[M::mem_pestat] mean and std.dev: (501.06, 348.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2567)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684582 reads in 149.782 CPU sec, 4.757 real sec
[M::process] read 695758 sequences (100000295 bp) in 1.726 CPU sec, 1.730 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282983, 1, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 223, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (257.17, 101.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (337.22, 160.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 284, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503)
[M::mem_pestat] mean and std.dev: (261.40, 81.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682906 reads in 87.726 CPU sec, 3.064 real sec
sorting block 33, #reads = 1076329 mem = 536879148 peak = 979222528
[M::mem_process_seqs] Processed 683580 reads in 204.923 CPU sec, 6.798 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283280, 10, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 279, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (339.20, 262.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1390)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 320, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (336.09, 163.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (832, 852, 1691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3409)
[M::mem_pestat] mean and std.dev: (991.20, 565.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4268)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 189, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (252.10, 154.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 34, #reads = 1075490 mem = 536879071 peak = 1018085376
[M::process] read 691524 sequences (100000285 bp) in 2.159 CPU sec, 2.268 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 287681, 7, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 257, 772)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2030)
[M::mem_pestat] mean and std.dev: (464.70, 416.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2659)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 253, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (282.09, 142.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 388, 747)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769)
[M::mem_pestat] mean and std.dev: (497.44, 373.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2280)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688912 sequences (100000012 bp) in 1.390 CPU sec, 1.386 real sec...
[M::mem_process_seqs] Processed 683640 reads in 170.268 CPU sec, 5.411 real sec
[M::mem_process_seqs] Processed 695758 reads in 104.473 CPU sec, 3.305 real sec
sorting block 35, #reads = 1073104 mem = 536883523 peak = 1018085376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286334, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 274, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (298.13, 148.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 325, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 486)
[M::mem_pestat] mean and std.dev: (306.17, 63.04)
[M::mem_pestat] low and high boundaries for proper pairs: (54, 558)
[M::mem_pestat] skip orientation RR
[M::process] read 687502 sequences (100000026 bp) in 2.352 CPU sec, 2.482 real sec...
[M::process] read 686744 sequences (100000033 bp) in 1.410 CPU sec, 1.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285603, 4, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 288, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (308.78, 151.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 576, 576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184)
[M::mem_pestat] mean and std.dev: (389.15, 155.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1488)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 691524 reads in 155.948 CPU sec, 4.951 real sec
[M::mem_process_seqs] Processed 688912 reads in 97.113 CPU sec, 3.086 real sec
sorting block 36, #reads = 1073826 mem = 536882254 peak = 1018085376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 285837, 15, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 297, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (356.68, 202.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 297, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (315.72, 154.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (618, 785, 785)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (284, 1119)
[M::mem_pestat] mean and std.dev: (670.46, 138.56)
[M::mem_pestat] low and high boundaries for proper pairs: (116, 1286)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 370, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1545)
[M::mem_pestat] mean and std.dev: (418.44, 260.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1997)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685604 sequences (100000077 bp) in 2.252 CPU sec, 2.365 real sec...
[M::process] read 684394 sequences (100000023 bp) in 1.400 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284538, 6, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 235, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (274.00, 145.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 300, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (318.74, 156.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 335, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159)
[M::mem_pestat] mean and std.dev: (413.20, 281.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1541)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687502 reads in 149.101 CPU sec, 4.736 real sec
[M::mem_process_seqs] Processed 686744 reads in 93.613 CPU sec, 2.962 real sec
sorting block 37, #reads = 1075820 mem = 536883010 peak = 1018085376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283937, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (257, 326, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (284.75, 113.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 309, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (327.42, 161.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683298 sequences (100000167 bp) in 2.331 CPU sec, 2.474 real sec...
[M::process] read 683010 sequences (100000049 bp) in 1.388 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283664, 10, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (97, 163, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (228.22, 148.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 317, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (335.62, 165.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (669, 5845, 5845)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16197)
[M::mem_pestat] mean and std.dev: (3680.20, 2378.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21373)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 258, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (311.43, 135.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685604 reads in 149.741 CPU sec, 4.764 real sec
sorting block 38, #reads = 1076168 mem = 536878682 peak = 1018085376
[M::mem_process_seqs] Processed 684394 reads in 93.913 CPU sec, 2.985 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282947, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 229, 841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2143)
[M::mem_pestat] mean and std.dev: (430.75, 388.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2794)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (339.50, 166.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 360, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (335.27, 94.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 39, #reads = 1076251 mem = 536878203 peak = 1068048384
[M::process] read 687628 sequences (100000192 bp) in 2.601 CPU sec, 2.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282897, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 243, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (234.78, 74.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 327, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (344.57, 170.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 222, 584)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346)
[M::mem_pestat] mean and std.dev: (245.46, 112.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1727)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683298 reads in 149.900 CPU sec, 4.767 real sec
[M::process] read 687794 sequences (100000202 bp) in 1.714 CPU sec, 1.745 real sec...
[M::mem_process_seqs] Processed 683010 reads in 95.556 CPU sec, 3.008 real sec
sorting block 40, #reads = 1075578 mem = 536880117 peak = 1068048384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284955, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 294, 696)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1686)
[M::mem_pestat] mean and std.dev: (411.56, 296.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2181)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 296, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (319.88, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 686314 sequences (100000301 bp) in 2.781 CPU sec, 2.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284674, 8, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (303, 358, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (77, 642)
[M::mem_pestat] mean and std.dev: (316.29, 106.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 755)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 295, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (317.05, 159.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 334, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712)
[M::mem_pestat] mean and std.dev: (266.80, 87.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685292 sequences (100000200 bp) in 1.370 CPU sec, 1.373 real sec...
[M::mem_process_seqs] Processed 687628 reads in 150.777 CPU sec, 4.792 real sec
[M::mem_process_seqs] Processed 687794 reads in 91.448 CPU sec, 2.891 real sec
sorting block 41, #reads = 1073342 mem = 536877926 peak = 1068048384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284399, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 296, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (245.42, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 304, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (323.21, 160.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 280, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204)
[M::mem_pestat] mean and std.dev: (314.00, 165.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1539)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684374 sequences (100000007 bp) in 2.386 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284315, 8, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (293, 349, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (163, 488)
[M::mem_pestat] mean and std.dev: (298.67, 73.12)
[M::mem_pestat] low and high boundaries for proper pairs: (6, 591)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 310, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (327.25, 160.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 442, 895)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2259)
[M::mem_pestat] mean and std.dev: (574.83, 399.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2941)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685564 sequences (100000284 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_process_seqs] Processed 686314 reads in 140.521 CPU sec, 4.467 real sec
sorting block 42, #reads = 1079586 mem = 536878375 peak = 1068048384
[M::mem_process_seqs] Processed 685292 reads in 88.200 CPU sec, 2.792 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283888, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 373, 708)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712)
[M::mem_pestat] mean and std.dev: (461.57, 292.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2214)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (331.30, 161.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 216, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153)
[M::mem_pestat] mean and std.dev: (278.38, 189.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1474)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 43, #reads = 1079727 mem = 536878403 peak = 1068048384
[M::process] read 684424 sequences (100000166 bp) in 2.122 CPU sec, 2.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284170, 2, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 254, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239)
[M::mem_pestat] mean and std.dev: (334.62, 188.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1573)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 308, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (327.11, 161.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 254, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (265.40, 95.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683674 sequences (100000170 bp) in 1.390 CPU sec, 1.388 real sec...
[M::mem_process_seqs] Processed 684374 reads in 139.836 CPU sec, 4.450 real sec
[M::mem_process_seqs] Processed 685564 reads in 88.737 CPU sec, 2.807 real sec
sorting block 44, #reads = 1079784 mem = 536879892 peak = 1068048384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284055, 4, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 333, 1505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4125)
[M::mem_pestat] mean and std.dev: (848.48, 901.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5435)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (333.37, 164.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 358, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (338.92, 213.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1390)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683686 sequences (100000227 bp) in 2.320 CPU sec, 2.424 real sec...
[M::process] read 683008 sequences (100000175 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283126, 5, 23)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (338.58, 166.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (305, 449, 749)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637)
[M::mem_pestat] mean and std.dev: (492.95, 360.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2081)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684424 reads in 142.038 CPU sec, 4.512 real sec
[M::mem_process_seqs] Processed 683674 reads in 89.905 CPU sec, 2.846 real sec
sorting block 45, #reads = 1078429 mem = 536878988 peak = 1068048384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283743, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 449, 615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1489)
[M::mem_pestat] mean and std.dev: (371.07, 222.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1926)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 318, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (337.27, 166.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (319, 549, 1210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2992)
[M::mem_pestat] mean and std.dev: (711.45, 503.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3883)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682940 sequences (100000286 bp) in 2.274 CPU sec, 2.385 real sec...
[M::process] read 694458 sequences (100000026 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283413, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 373, 821)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2033)
[M::mem_pestat] mean and std.dev: (574.37, 425.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2639)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (339.87, 166.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 322, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (330.32, 180.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683686 reads in 144.893 CPU sec, 4.600 real sec
[M::mem_process_seqs] Processed 683008 reads in 92.061 CPU sec, 2.914 real sec
sorting block 46, #reads = 1077005 mem = 536877687 peak = 1068048384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282899, 0, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 253, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (302.25, 154.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 323, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (341.72, 168.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 287, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1593)
[M::mem_pestat] mean and std.dev: (424.72, 330.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2049)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689984 sequences (100000019 bp) in 2.302 CPU sec, 2.406 real sec...
[M::process] read 687336 sequences (100000228 bp) in 1.350 CPU sec, 1.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 287213, 9, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 219, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149)
[M::mem_pestat] mean and std.dev: (312.58, 255.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1482)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (290.70, 149.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 269, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (265.33, 94.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682940 reads in 158.888 CPU sec, 5.052 real sec
sorting block 47, #reads = 1075620 mem = 536880870 peak = 1068048384
[M::mem_process_seqs] Processed 694458 reads in 104.024 CPU sec, 3.306 real sec
sorting block 48, #reads = 1070573 mem = 536878154 peak = 1068048384
[M::process] read 685852 sequences (100000088 bp) in 2.313 CPU sec, 2.426 real sec...
[M::process] read 684806 sequences (100000220 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284946, 6, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 299, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (236.88, 80.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 301, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (319.46, 156.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 1081)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2851)
[M::mem_pestat] mean and std.dev: (647.38, 607.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3736)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687336 reads in 89.902 CPU sec, 2.866 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 286045, 8, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 271, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (258.28, 74.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 284, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (307.45, 154.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 313, 1032)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2724)
[M::mem_pestat] mean and std.dev: (678.71, 812.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3927)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 284825, 9, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 195, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (220.19, 107.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 683)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 309, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (326.61, 159.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 350, 653)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671)
[M::mem_pestat] mean and std.dev: (374.70, 246.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2180)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684154 sequences (100000021 bp) in 1.415 CPU sec, 1.425 real sec...
[M::mem_process_seqs] Processed 689984 reads in 239.064 CPU sec, 7.607 real sec
sorting block 49, #reads = 1073547 mem = 536881413 peak = 1068048384
[M::mem_process_seqs] Processed 685852 reads in 112.207 CPU sec, 3.586 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283876, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (238, 405, 603)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1333)
[M::mem_pestat] mean and std.dev: (367.42, 172.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1698)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 316, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (332.56, 162.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 50, #reads = 1073916 mem = 536878134 peak = 1068048384
[M::process] read 683366 sequences (100000035 bp) in 2.500 CPU sec, 2.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283325, 9, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 283, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (327.85, 168.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (333.49, 161.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 482, 859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085)
[M::mem_pestat] mean and std.dev: (476.43, 267.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2698)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682804 sequences (100000130 bp) in 1.350 CPU sec, 1.343 real sec...
[M::mem_process_seqs] Processed 684806 reads in 150.984 CPU sec, 4.801 real sec
[M::mem_process_seqs] Processed 684154 reads in 95.898 CPU sec, 3.038 real sec
sorting block 51, #reads = 1073738 mem = 536877954 peak = 1068048384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283510, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 396, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1639)
[M::mem_pestat] mean and std.dev: (537.53, 460.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2379)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 319, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (336.85, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 384, 714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1660)
[M::mem_pestat] mean and std.dev: (391.69, 229.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2133)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682588 sequences (100000209 bp) in 2.204 CPU sec, 2.335 real sec...
[M::process] read 648636 sequences (95066508 bp) in 1.280 CPU sec, 1.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283249, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 339, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (281.00, 150.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (338.54, 163.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 295, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (282.82, 124.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683366 reads in 146.514 CPU sec, 4.661 real sec
[M::mem_process_seqs] Processed 682804 reads in 91.891 CPU sec, 2.927 real sec
sorting block 52, #reads = 1074712 mem = 536879705 peak = 1068048384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282896, 3, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 207, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 514)
[M::mem_pestat] mean and std.dev: (231.46, 103.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 646)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 325, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (343.00, 167.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 324, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265)
[M::mem_pestat] mean and std.dev: (314.43, 159.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1626)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 268784, 1, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 272, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (298.67, 133.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 328, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (345.79, 169.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 282, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073)
[M::mem_pestat] mean and std.dev: (316.76, 177.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1368)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682588 reads in 136.391 CPU sec, 4.419 real sec
[M::mem_process_seqs] Processed 648636 reads in 84.549 CPU sec, 2.737 real sec
sorting block 53, #reads = 1075022 mem = 536879625 peak = 1094995968
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 58846560
overall: 69892714496 mem 8196.102 user 365.784 sys 836.519 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 836.559 sec; CPU: 8561.892 sec
sorting block 54, #reads = 951601 mem = 474528117 peak = 1165819904
uniq header count 1
merging 55 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3603168773
output reads: 59160811
bam_mem_sort: 55 calls 20.984 user 0.284 sys 21.394 real
bam_write: 55 calls 29.552 user 0.708 sys 30.515 real
execute: 1 calls 10.978 user 0.538 sys 857.712 real
merge_files: 1 calls 10.964 user 0.505 sys 19.981 real
parse_chunk: 879 calls 29.642 user 7.464 sys 37.733 real
read_chunk: 2372 calls 20.607 user 21.327 sys 224.938 real
sort_block: 1 calls 16.676 user 20.825 sys 218.939 real
write_chunk: 1491 calls 10.649 user 0.331 sys 19.284 real
overall: 2664980480 mem 750.738 user 36.253 sys 857.738 real
ls: cannot access '*.cram': No such file or directory