This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_dm4-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2736.247 CPU sec, 109.764 real sec [M::process] read 754990 sequences (100000273 bp) in 1.419 CPU sec, 1.429 real sec... [M::process] read 755706 sequences (100000139 bp) in 1.929 CPU sec, 1.944 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 303019, 28, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 234, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2064) [M::mem_pestat] mean and std.dev: (374.45, 419.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.32, 69.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 506, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3937) [M::mem_pestat] mean and std.dev: (772.88, 823.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 424, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2922) [M::mem_pestat] mean and std.dev: (820.57, 827.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755528 sequences (100000138 bp) in 2.032 CPU sec, 2.089 real sec... [M::process] read 755180 sequences (100000009 bp) in 2.078 CPU sec, 2.134 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 304378, 20, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 451, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588) [M::mem_pestat] mean and std.dev: (565.05, 598.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (168.24, 69.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 839, 1111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3079) [M::mem_pestat] mean and std.dev: (647.67, 547.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 269, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2780) [M::mem_pestat] mean and std.dev: (575.33, 523.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754990 reads in 223.730 CPU sec, 7.115 real sec [M::mem_process_seqs] Processed 755706 reads in 141.429 CPU sec, 4.554 real sec sorting block 0, #reads = 1117010 mem = 536879536 peak = 564314112 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 303899, 19, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 217, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2445) [M::mem_pestat] mean and std.dev: (508.93, 580.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (168.64, 70.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 387, 2614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7634) [M::mem_pestat] mean and std.dev: (1340.95, 1606.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10144) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 300, 2441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7001) [M::mem_pestat] mean and std.dev: (1081.59, 1522.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754960 sequences (100000012 bp) in 3.537 CPU sec, 3.866 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 304430, 22, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 335, 1314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3584) [M::mem_pestat] mean and std.dev: (806.63, 828.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::process] read 754100 sequences (100000271 bp) in 1.932 CPU sec, 1.939 real sec... [M::mem_pestat] mean and std.dev: (168.72, 70.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (387, 560, 3131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8619) [M::mem_pestat] mean and std.dev: (1463.64, 1520.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 184, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1835) [M::mem_pestat] mean and std.dev: (414.08, 385.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755528 reads in 216.279 CPU sec, 6.901 real sec [M::mem_process_seqs] Processed 755180 reads in 149.930 CPU sec, 4.814 real sec sorting block 1, #reads = 1118544 mem = 536882579 peak = 709017600 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 304212, 19, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 381, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1893) [M::mem_pestat] mean and std.dev: (468.05, 372.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (168.82, 70.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 580, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3298) [M::mem_pestat] mean and std.dev: (495.18, 525.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 402, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2265) [M::mem_pestat] mean and std.dev: (575.83, 500.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755424 sequences (100000189 bp) in 3.482 CPU sec, 3.889 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 303733, 17, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 162, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124) [M::mem_pestat] mean and std.dev: (263.77, 234.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (168.79, 70.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (481, 919, 2671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7051) [M::mem_pestat] mean and std.dev: (1580.35, 1514.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 463, 1519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4205) [M::mem_pestat] mean and std.dev: (748.10, 843.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5548) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754960 reads in 221.933 CPU sec, 7.156 real sec [M::mem_process_seqs] Processed 754100 reads in 145.430 CPU sec, 4.638 real sec [M::process] read 752932 sequences (100000262 bp) in 2.853 CPU sec, 3.028 real sec... sorting block 2, #reads = 1120194 mem = 536877821 peak = 742666240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 304490, 14, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (494.25, 535.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (168.11, 69.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 991, 2521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6949) [M::mem_pestat] mean and std.dev: (1144.86, 1018.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9163) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 211, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (262.55, 190.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751404 sequences (100000023 bp) in 4.096 CPU sec, 4.548 real sec... sorting block 3, #reads = 1118401 mem = 536878443 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 303323, 31, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 479, 2017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5597) [M::mem_pestat] mean and std.dev: (904.02, 1064.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (170.70, 71.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (466, 635, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2848) [M::mem_pestat] mean and std.dev: (671.12, 450.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3642) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 258, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1452) [M::mem_pestat] mean and std.dev: (314.88, 277.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755424 reads in 227.248 CPU sec, 7.350 real sec [M::mem_process_seqs] Processed 752932 reads in 144.548 CPU sec, 4.623 real sec [M::process] read 750830 sequences (100000222 bp) in 2.925 CPU sec, 3.117 real sec... sorting block 4, #reads = 1118530 mem = 536879715 peak = 786268160 [M::process] read 750652 sequences (100000082 bp) in 3.326 CPU sec, 3.630 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 302506, 19, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 362, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2228) [M::mem_pestat] mean and std.dev: (550.40, 489.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (172.43, 72.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 397, 2520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7152) [M::mem_pestat] mean and std.dev: (1291.37, 1711.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 391, 1090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2932) [M::mem_pestat] mean and std.dev: (633.52, 661.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 750830 reads in 128.654 CPU sec, 4.610 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 302208, 29, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 327, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2708) [M::mem_pestat] mean and std.dev: (650.98, 700.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (172.52, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 621, 1743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4749) [M::mem_pestat] mean and std.dev: (1191.90, 1365.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 353, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1919) [M::mem_pestat] mean and std.dev: (422.41, 354.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2504) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749900 sequences (100000082 bp) in 3.329 CPU sec, 3.668 real sec... sorting block 5, #reads = 1116727 mem = 536875973 peak = 786268160 [M::mem_process_seqs] Processed 750652 reads in 133.512 CPU sec, 4.911 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 303038, 24, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 458, 1450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3914) [M::mem_pestat] mean and std.dev: (870.63, 820.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.52, 72.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (141, 360, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2127) [M::mem_pestat] mean and std.dev: (370.71, 330.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 283, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1453) [M::mem_pestat] mean and std.dev: (296.12, 161.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 748842 sequences (100000029 bp) in 3.238 CPU sec, 3.545 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 302374, 25, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 335, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2113) [M::mem_pestat] mean and std.dev: (538.59, 526.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.40, 72.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (439, 527, 2366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6220) [M::mem_pestat] mean and std.dev: (1204.04, 1407.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8147) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 252, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1332) [M::mem_pestat] mean and std.dev: (352.16, 279.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 751404 reads in 514.422 CPU sec, 17.960 real sec [M::mem_process_seqs] Processed 749900 reads in 155.458 CPU sec, 5.239 real sec sorting block 6, #reads = 1116753 mem = 536880157 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 301346, 22, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 228, 1773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4951) [M::mem_pestat] mean and std.dev: (637.47, 862.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.54, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 224, 1371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3953) [M::mem_pestat] mean and std.dev: (513.58, 835.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5244) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 370, 1892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5274) [M::mem_pestat] mean and std.dev: (722.07, 895.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747692 sequences (100000016 bp) in 3.770 CPU sec, 4.222 real sec... [M::mem_process_seqs] Processed 748842 reads in 139.210 CPU sec, 5.322 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 301221, 29, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 247, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1600) [M::mem_pestat] mean and std.dev: (379.94, 342.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.86, 73.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 712, 1567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4363) [M::mem_pestat] mean and std.dev: (972.93, 841.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 493, 1468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4064) [M::mem_pestat] mean and std.dev: (844.25, 954.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 748020 sequences (100000123 bp) in 4.158 CPU sec, 4.609 real sec... sorting block 7, #reads = 1116799 mem = 536880171 peak = 786268160 [M::mem_process_seqs] Processed 747692 reads in 144.281 CPU sec, 5.444 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 301627, 21, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 275, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2744) [M::mem_pestat] mean and std.dev: (563.84, 633.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.74, 73.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 594, 1797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5079) [M::mem_pestat] mean and std.dev: (856.90, 827.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 326, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2300) [M::mem_pestat] mean and std.dev: (461.79, 390.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 745704 sequences (100000247 bp) in 3.808 CPU sec, 4.292 real sec... [M::mem_process_seqs] Processed 748020 reads in 144.752 CPU sec, 5.560 real sec sorting block 8, #reads = 1114584 mem = 536878242 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 300696, 26, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 462, 1996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5700) [M::mem_pestat] mean and std.dev: (1100.71, 1353.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (176.56, 74.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 814, 1805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4831) [M::mem_pestat] mean and std.dev: (1134.88, 1039.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 342, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3228) [M::mem_pestat] mean and std.dev: (580.25, 461.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 745704 reads in 126.653 CPU sec, 4.424 real sec [M::process] read 745840 sequences (100000200 bp) in 4.631 CPU sec, 5.181 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 300457, 23, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 268, 1036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2732) [M::mem_pestat] mean and std.dev: (599.46, 634.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (176.29, 74.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 182, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2063) [M::mem_pestat] mean and std.dev: (339.71, 460.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2721) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 394, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3700) [M::mem_pestat] mean and std.dev: (428.93, 396.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 9, #reads = 1115424 mem = 536879949 peak = 786268160 [M::process] read 746340 sequences (100000082 bp) in 3.861 CPU sec, 4.261 real sec... [M::mem_process_seqs] Processed 745840 reads in 129.821 CPU sec, 4.763 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 300717, 20, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 381, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2125) [M::mem_pestat] mean and std.dev: (494.42, 478.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.43, 74.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 551, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3220) [M::mem_pestat] mean and std.dev: (736.90, 597.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4206) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 250, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2620) [M::mem_pestat] mean and std.dev: (491.89, 484.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 746064 sequences (100000091 bp) in 3.730 CPU sec, 4.132 real sec... [M::mem_process_seqs] Processed 746340 reads in 129.995 CPU sec, 4.429 real sec sorting block 10, #reads = 1113232 mem = 536879755 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 300988, 20, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 272, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2055) [M::mem_pestat] mean and std.dev: (408.86, 410.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.61, 73.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (271, 815, 1674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4480) [M::mem_pestat] mean and std.dev: (851.68, 807.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5883) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 272, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1369) [M::mem_pestat] mean and std.dev: (370.57, 330.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 746134 sequences (100000241 bp) in 4.084 CPU sec, 4.511 real sec... [M::mem_process_seqs] Processed 746064 reads in 129.467 CPU sec, 5.208 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 300737, 19, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 425, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4678) [M::mem_pestat] mean and std.dev: (1154.29, 1346.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.64, 73.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (456, 536, 1509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3615) [M::mem_pestat] mean and std.dev: (895.63, 838.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 317, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827) [M::mem_pestat] mean and std.dev: (614.82, 576.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 746134 reads in 125.469 CPU sec, 4.218 real sec sorting block 11, #reads = 1111373 mem = 536879160 peak = 786268160 [M::process] read 745398 sequences (100000137 bp) in 4.462 CPU sec, 4.883 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 300560, 34, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 256, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (260.62, 203.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (176.09, 74.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 622, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2464) [M::mem_pestat] mean and std.dev: (658.22, 558.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1691) [M::mem_pestat] mean and std.dev: (369.73, 280.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 745398 reads in 128.440 CPU sec, 4.443 real sec [M::process] read 744730 sequences (100000142 bp) in 4.430 CPU sec, 4.973 real sec... sorting block 12, #reads = 1113828 mem = 536879440 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 300049, 28, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 297, 1840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5234) [M::mem_pestat] mean and std.dev: (784.86, 1020.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (176.88, 74.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 626, 1566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4158) [M::mem_pestat] mean and std.dev: (877.11, 787.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5454) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 249, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (292.26, 214.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1590) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 744400 sequences (100000139 bp) in 4.203 CPU sec, 4.676 real sec... [M::mem_process_seqs] Processed 744730 reads in 131.842 CPU sec, 5.114 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 300236, 26, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242) [M::mem_pestat] mean and std.dev: (293.40, 190.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 164, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (177.57, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 450, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1647) [M::mem_pestat] mean and std.dev: (493.04, 376.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2103) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 314, 1364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3722) [M::mem_pestat] mean and std.dev: (472.44, 475.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4901) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 744400 reads in 132.839 CPU sec, 4.635 real sec sorting block 13, #reads = 1112492 mem = 536887153 peak = 786268160 [M::process] read 751682 sequences (100000066 bp) in 4.610 CPU sec, 5.000 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 302595, 23, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 392, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2636) [M::mem_pestat] mean and std.dev: (622.31, 678.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.66, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (83, 232, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646) [M::mem_pestat] mean and std.dev: (369.83, 331.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2167) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 303, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172) [M::mem_pestat] mean and std.dev: (501.42, 450.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 751682 reads in 126.037 CPU sec, 5.063 real sec [M::process] read 756278 sequences (100000080 bp) in 5.510 CPU sec, 6.003 real sec... sorting block 14, #reads = 1112649 mem = 536880112 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 304506, 25, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 237, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1544) [M::mem_pestat] mean and std.dev: (312.90, 289.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (167.86, 69.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 473, 3187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9141) [M::mem_pestat] mean and std.dev: (1839.20, 2730.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2830) [M::mem_pestat] mean and std.dev: (624.39, 735.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 756278 reads in 123.364 CPU sec, 4.271 real sec [M::process] read 756172 sequences (100000133 bp) in 3.996 CPU sec, 4.446 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 303964, 23, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 323, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3139) [M::mem_pestat] mean and std.dev: (527.57, 467.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (168.17, 70.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 265, 1256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3328) [M::mem_pestat] mean and std.dev: (559.19, 613.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 419, 1073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2797) [M::mem_pestat] mean and std.dev: (584.86, 468.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 15, #reads = 1111672 mem = 536879712 peak = 786268160 [M::mem_process_seqs] Processed 756172 reads in 126.252 CPU sec, 4.384 real sec [M::process] read 755896 sequences (100000121 bp) in 4.233 CPU sec, 4.722 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 304700, 19, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 269, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2529) [M::mem_pestat] mean and std.dev: (467.77, 429.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (168.11, 70.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 294, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2075) [M::mem_pestat] mean and std.dev: (369.06, 381.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 199, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (214.81, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755204 sequences (100000143 bp) in 4.318 CPU sec, 4.774 real sec... [M::mem_process_seqs] Processed 755896 reads in 122.546 CPU sec, 4.860 real sec sorting block 16, #reads = 1120469 mem = 536884012 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 303776, 22, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 411, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4677) [M::mem_pestat] mean and std.dev: (580.47, 561.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (168.75, 70.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (271, 1061, 2134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5860) [M::mem_pestat] mean and std.dev: (1026.65, 865.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 308, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1785) [M::mem_pestat] mean and std.dev: (301.36, 242.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754460 sequences (100000273 bp) in 3.589 CPU sec, 3.985 real sec... [M::mem_process_seqs] Processed 755204 reads in 123.850 CPU sec, 4.344 real sec sorting block 17, #reads = 1119964 mem = 536877743 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 304004, 26, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 362, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2349) [M::mem_pestat] mean and std.dev: (458.26, 338.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.43, 71.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (264, 466, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2343) [M::mem_pestat] mean and std.dev: (363.80, 245.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 213, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3247) [M::mem_pestat] mean and std.dev: (489.24, 507.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 753972 sequences (100000150 bp) in 3.372 CPU sec, 3.865 real sec... [M::mem_process_seqs] Processed 754460 reads in 118.719 CPU sec, 4.302 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 303379, 31, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 211, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2582) [M::mem_pestat] mean and std.dev: (496.21, 422.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.66, 71.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (171, 484, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3873) [M::mem_pestat] mean and std.dev: (816.97, 829.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5107) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 278, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1484) [M::mem_pestat] mean and std.dev: (321.84, 221.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 753908 sequences (100000203 bp) in 3.367 CPU sec, 3.756 real sec... sorting block 18, #reads = 1120113 mem = 536876724 peak = 786268160 [M::mem_process_seqs] Processed 753972 reads in 120.228 CPU sec, 4.131 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 303907, 17, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 306, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3392) [M::mem_pestat] mean and std.dev: (575.61, 614.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.61, 71.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (110, 196, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2369) [M::mem_pestat] mean and std.dev: (395.56, 409.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3122) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 316, 1198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3280) [M::mem_pestat] mean and std.dev: (708.61, 762.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 752428 sequences (100000117 bp) in 3.154 CPU sec, 3.545 real sec... [M::mem_process_seqs] Processed 753908 reads in 117.003 CPU sec, 3.984 real sec sorting block 19, #reads = 1118800 mem = 536886048 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 302317, 27, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 308, 1502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4168) [M::mem_pestat] mean and std.dev: (776.58, 993.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (171.22, 72.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 377, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3322) [M::mem_pestat] mean and std.dev: (749.96, 671.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 303, 1634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4422) [M::mem_pestat] mean and std.dev: (1067.11, 1256.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751412 sequences (100000155 bp) in 3.273 CPU sec, 3.621 real sec... [M::mem_process_seqs] Processed 752428 reads in 115.559 CPU sec, 3.981 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 302683, 15, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 233, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2032) [M::mem_pestat] mean and std.dev: (415.76, 378.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.74, 72.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (123, 174, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2520) [M::mem_pestat] mean and std.dev: (302.77, 362.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 383, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2667) [M::mem_pestat] mean and std.dev: (465.45, 365.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3495) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750728 sequences (100000010 bp) in 3.273 CPU sec, 3.689 real sec... sorting block 20, #reads = 1118656 mem = 536889928 peak = 786268160 [M::mem_process_seqs] Processed 751412 reads in 119.441 CPU sec, 4.179 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 302721, 25, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 245, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2140) [M::mem_pestat] mean and std.dev: (483.73, 465.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (172.53, 72.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (239, 945, 1713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4661) [M::mem_pestat] mean and std.dev: (897.73, 677.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 265, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (351.34, 270.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750522 sequences (100000022 bp) in 3.432 CPU sec, 3.736 real sec... [M::mem_process_seqs] Processed 750728 reads in 116.766 CPU sec, 4.164 real sec sorting block 21, #reads = 1116809 mem = 536878433 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 302091, 15, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 170, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (173.94, 65.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (173.14, 73.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 235, 3520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10258) [M::mem_pestat] mean and std.dev: (1521.00, 2389.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 308, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907) [M::mem_pestat] mean and std.dev: (391.64, 333.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749648 sequences (100000163 bp) in 3.165 CPU sec, 3.579 real sec... [M::mem_process_seqs] Processed 750522 reads in 117.640 CPU sec, 4.070 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 301869, 13, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 358, 1380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3682) [M::mem_pestat] mean and std.dev: (707.12, 584.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.47, 73.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (135, 259, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1977) [M::mem_pestat] mean and std.dev: (479.23, 563.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2731) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 408, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4848) [M::mem_pestat] mean and std.dev: (712.06, 864.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747338 sequences (100000258 bp) in 3.419 CPU sec, 4.005 real sec... [M::mem_process_seqs] Processed 749648 reads in 117.250 CPU sec, 4.235 real sec sorting block 22, #reads = 1115840 mem = 536879079 peak = 786268160 [M::process] read 747354 sequences (100000215 bp) in 2.712 CPU sec, 3.101 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 301340, 37, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 284, 2160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6184) [M::mem_pestat] mean and std.dev: (822.18, 1072.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.31, 74.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (125, 516, 2233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6449) [M::mem_pestat] mean and std.dev: (751.78, 1099.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 255, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (284.16, 155.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747338 reads in 114.335 CPU sec, 4.001 real sec sorting block 23, #reads = 1117463 mem = 536880314 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 301491, 22, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 449, 1826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5096) [M::mem_pestat] mean and std.dev: (808.40, 902.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.48, 74.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 383, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3059) [M::mem_pestat] mean and std.dev: (600.25, 608.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 405, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2356) [M::mem_pestat] mean and std.dev: (538.49, 409.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747336 sequences (100000163 bp) in 3.278 CPU sec, 3.668 real sec... [M::mem_process_seqs] Processed 747354 reads in 118.727 CPU sec, 4.075 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 301137, 31, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 274, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1390) [M::mem_pestat] mean and std.dev: (353.81, 298.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.97, 74.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (405, 1190, 2145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5625) [M::mem_pestat] mean and std.dev: (1337.07, 1254.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 297, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141) [M::mem_pestat] mean and std.dev: (326.17, 210.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 24, #reads = 1116532 mem = 536879664 peak = 786268160 [M::process] read 745796 sequences (100000108 bp) in 3.194 CPU sec, 3.597 real sec... [M::mem_process_seqs] Processed 747336 reads in 115.002 CPU sec, 3.934 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 300899, 18, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 414, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3247) [M::mem_pestat] mean and std.dev: (677.36, 589.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (176.50, 74.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 562, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1755) [M::mem_pestat] mean and std.dev: (470.06, 300.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 294, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3357) [M::mem_pestat] mean and std.dev: (626.00, 739.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 745518 sequences (100000165 bp) in 3.155 CPU sec, 3.515 real sec... [M::mem_process_seqs] Processed 745796 reads in 122.243 CPU sec, 4.198 real sec sorting block 25, #reads = 1113969 mem = 536878734 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 300575, 33, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 277, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1897) [M::mem_pestat] mean and std.dev: (389.52, 406.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (176.19, 74.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 655, 1314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3578) [M::mem_pestat] mean and std.dev: (847.44, 794.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 863, 1445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3987) [M::mem_pestat] mean and std.dev: (933.42, 802.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 746272 sequences (100000182 bp) in 2.988 CPU sec, 3.347 real sec... [M::mem_process_seqs] Processed 745518 reads in 115.068 CPU sec, 3.993 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 300892, 19, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 359, 2729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7925) [M::mem_pestat] mean and std.dev: (1080.43, 1429.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.48, 73.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 418, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2118) [M::mem_pestat] mean and std.dev: (525.00, 519.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2745) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 248, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1600) [M::mem_pestat] mean and std.dev: (310.88, 235.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 26, #reads = 1114572 mem = 536878932 peak = 786268160 [M::process] read 746006 sequences (100000037 bp) in 3.672 CPU sec, 4.095 real sec... [M::mem_process_seqs] Processed 746272 reads in 120.175 CPU sec, 4.227 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 300856, 27, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 342, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1848) [M::mem_pestat] mean and std.dev: (444.90, 325.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.56, 74.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 227, 1020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2822) [M::mem_pestat] mean and std.dev: (220.14, 242.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 302, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1360) [M::mem_pestat] mean and std.dev: (368.69, 260.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 745618 sequences (100000056 bp) in 3.087 CPU sec, 3.395 real sec... [M::mem_process_seqs] Processed 746006 reads in 115.848 CPU sec, 4.068 real sec sorting block 27, #reads = 1112513 mem = 536883209 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 300437, 30, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 258, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934) [M::mem_pestat] mean and std.dev: (348.29, 390.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (120, 163, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (176.27, 74.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 1049, 1942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5492) [M::mem_pestat] mean and std.dev: (1135.73, 1055.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 446, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3055) [M::mem_pestat] mean and std.dev: (733.04, 715.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 745536 sequences (100000066 bp) in 3.288 CPU sec, 3.679 real sec... [M::mem_process_seqs] Processed 745618 reads in 118.220 CPU sec, 4.075 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 300754, 26, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2383) [M::mem_pestat] mean and std.dev: (542.11, 484.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 163, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (176.03, 74.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (125, 585, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3665) [M::mem_pestat] mean and std.dev: (845.08, 1057.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5074) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1621) [M::mem_pestat] mean and std.dev: (343.06, 333.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 746440 sequences (100000005 bp) in 3.078 CPU sec, 3.530 real sec... sorting block 28, #reads = 1112022 mem = 536883672 peak = 786268160 [M::mem_process_seqs] Processed 745536 reads in 119.542 CPU sec, 4.143 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 300526, 27, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 462, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907) [M::mem_pestat] mean and std.dev: (441.53, 342.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (175.08, 73.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (358, 733, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2866) [M::mem_pestat] mean and std.dev: (706.21, 522.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3702) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 232, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790) [M::mem_pestat] mean and std.dev: (653.71, 683.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 748748 sequences (100000076 bp) in 3.068 CPU sec, 3.435 real sec... [M::mem_process_seqs] Processed 746440 reads in 116.656 CPU sec, 3.991 real sec sorting block 29, #reads = 1112391 mem = 536877406 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 301641, 25, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 305, 1403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3979) [M::mem_pestat] mean and std.dev: (633.84, 709.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (173.93, 73.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 446, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5112) [M::mem_pestat] mean and std.dev: (1044.16, 1217.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6752) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 347, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1758) [M::mem_pestat] mean and std.dev: (449.59, 381.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 757886 sequences (100000008 bp) in 3.318 CPU sec, 3.710 real sec... [M::mem_process_seqs] Processed 748748 reads in 120.431 CPU sec, 4.125 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 305036, 26, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 420, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3212) [M::mem_pestat] mean and std.dev: (572.09, 541.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4225) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 30, #reads = 1111232 mem = 536877416 peak = 786268160 [M::mem_pestat] (25, 50, 75) percentile: (114, 154, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (167.13, 69.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 614, 1314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3460) [M::mem_pestat] mean and std.dev: (821.42, 708.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 221, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (248.86, 140.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 756436 sequences (100000255 bp) in 3.353 CPU sec, 3.704 real sec... [M::mem_process_seqs] Processed 757886 reads in 121.972 CPU sec, 4.111 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 304426, 25, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 257, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2022) [M::mem_pestat] mean and std.dev: (470.68, 499.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (167.77, 69.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 468, 1890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5528) [M::mem_pestat] mean and std.dev: (870.00, 1167.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 212, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2234) [M::mem_pestat] mean and std.dev: (462.03, 498.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755580 sequences (100000095 bp) in 3.309 CPU sec, 3.683 real sec... [M::mem_process_seqs] Processed 756436 reads in 118.868 CPU sec, 4.073 real sec sorting block 31, #reads = 1114024 mem = 536878591 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 303770, 18, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 465, 2020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5590) [M::mem_pestat] mean and std.dev: (943.16, 995.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (168.74, 70.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (476, 925, 5215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14693) [M::mem_pestat] mean and std.dev: (2363.72, 2890.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 326, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579) [M::mem_pestat] mean and std.dev: (456.17, 440.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754716 sequences (100000087 bp) in 3.626 CPU sec, 3.989 real sec... [M::mem_process_seqs] Processed 755580 reads in 122.101 CPU sec, 4.180 real sec sorting block 32, #reads = 1118593 mem = 536878513 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 303908, 30, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 341, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1638) [M::mem_pestat] mean and std.dev: (405.36, 319.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.10, 70.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (147, 391, 1669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4713) [M::mem_pestat] mean and std.dev: (1031.59, 1257.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 228, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (326.26, 238.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754810 sequences (100000028 bp) in 3.136 CPU sec, 3.493 real sec... [M::mem_process_seqs] Processed 754716 reads in 117.290 CPU sec, 4.203 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 303087, 31, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 189, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2760) [M::mem_pestat] mean and std.dev: (454.20, 589.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.42, 71.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (131, 332, 1679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4775) [M::mem_pestat] mean and std.dev: (1104.32, 1151.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6323) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 425, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3975) [M::mem_pestat] mean and std.dev: (734.96, 746.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5239) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 753712 sequences (100000196 bp) in 3.271 CPU sec, 3.675 real sec... [M::mem_process_seqs] Processed 754810 reads in 119.308 CPU sec, 4.305 real sec sorting block 33, #reads = 1119536 mem = 536883298 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 303283, 25, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 194, 1181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3247) [M::mem_pestat] mean and std.dev: (666.41, 772.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (170.33, 71.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (304, 530, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2314) [M::mem_pestat] mean and std.dev: (630.96, 527.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2984) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 209, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2369) [M::mem_pestat] mean and std.dev: (411.87, 490.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 753096 sequences (100000072 bp) in 3.355 CPU sec, 3.784 real sec... [M::mem_process_seqs] Processed 753712 reads in 120.227 CPU sec, 4.385 real sec sorting block 34, #reads = 1119328 mem = 536878280 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 303357, 22, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 262, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (328.91, 250.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (170.96, 72.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (111, 577, 1171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3291) [M::mem_pestat] mean and std.dev: (612.67, 583.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4351) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2025) [M::mem_pestat] mean and std.dev: (417.07, 423.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 752896 sequences (100000220 bp) in 3.673 CPU sec, 4.106 real sec... [M::mem_process_seqs] Processed 753096 reads in 118.740 CPU sec, 4.629 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 303289, 15, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 350, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3330) [M::mem_pestat] mean and std.dev: (671.59, 721.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (170.91, 72.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 688, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2238) [M::mem_pestat] mean and std.dev: (711.33, 484.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2904) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 221, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1798) [M::mem_pestat] mean and std.dev: (336.14, 378.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751156 sequences (100000100 bp) in 3.341 CPU sec, 3.732 real sec... [M::mem_process_seqs] Processed 752896 reads in 119.107 CPU sec, 4.160 real sec sorting block 35, #reads = 1117226 mem = 536884179 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 303184, 18, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 339, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2222) [M::mem_pestat] mean and std.dev: (494.02, 477.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.78, 72.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (232, 1050, 3232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9232) [M::mem_pestat] mean and std.dev: (1139.47, 1212.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 272, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1293) [M::mem_pestat] mean and std.dev: (288.92, 187.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750924 sequences (100000208 bp) in 3.308 CPU sec, 3.763 real sec... [M::mem_process_seqs] Processed 751156 reads in 118.105 CPU sec, 4.133 real sec sorting block 36, #reads = 1117501 mem = 536878669 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 302609, 19, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 215, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (239.13, 144.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (172.41, 72.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (134, 488, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2507) [M::mem_pestat] mean and std.dev: (685.05, 729.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3603) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 265, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2444) [M::mem_pestat] mean and std.dev: (482.79, 508.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3209) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750936 sequences (100000011 bp) in 3.151 CPU sec, 3.537 real sec... [M::mem_process_seqs] Processed 750924 reads in 116.903 CPU sec, 4.025 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 302567, 22, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 220, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240) [M::mem_pestat] mean and std.dev: (296.56, 226.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (172.42, 72.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 598, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2617) [M::mem_pestat] mean and std.dev: (537.26, 576.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3441) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 221, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (246.97, 165.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750034 sequences (100000151 bp) in 3.153 CPU sec, 3.487 real sec... [M::mem_process_seqs] Processed 750936 reads in 115.426 CPU sec, 3.936 real sec sorting block 37, #reads = 1116410 mem = 536878061 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 301952, 21, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 349, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1897) [M::mem_pestat] mean and std.dev: (423.70, 345.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (173.14, 73.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 697, 2971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8513) [M::mem_pestat] mean and std.dev: (1311.57, 1465.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 219, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1797) [M::mem_pestat] mean and std.dev: (355.06, 345.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 748666 sequences (100000029 bp) in 3.075 CPU sec, 3.618 real sec... [M::mem_process_seqs] Processed 750034 reads in 120.781 CPU sec, 4.150 real sec sorting block 38, #reads = 1115350 mem = 536879151 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 301452, 22, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 421, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2539) [M::mem_pestat] mean and std.dev: (495.47, 474.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.46, 72.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 595, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3004) [M::mem_pestat] mean and std.dev: (675.33, 623.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 218, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (290.23, 184.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 748834 sequences (100000017 bp) in 2.890 CPU sec, 3.177 real sec... [M::mem_process_seqs] Processed 748666 reads in 115.485 CPU sec, 4.089 real sec [M::process] read 749078 sequences (100000254 bp) in 3.271 CPU sec, 3.753 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 301648, 20, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 505, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2462) [M::mem_pestat] mean and std.dev: (610.38, 523.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.22, 72.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 1050, 3226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9290) [M::mem_pestat] mean and std.dev: (1598.75, 1838.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 478, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2854) [M::mem_pestat] mean and std.dev: (721.33, 636.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 39, #reads = 1115727 mem = 536879362 peak = 786268160 [M::mem_process_seqs] Processed 748834 reads in 121.874 CPU sec, 4.141 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 301481, 42, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 321, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1605) [M::mem_pestat] mean and std.dev: (356.59, 333.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.76, 72.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 844, 1862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5182) [M::mem_pestat] mean and std.dev: (1198.47, 1210.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6842) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 216, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646) [M::mem_pestat] mean and std.dev: (327.67, 252.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747208 sequences (100000267 bp) in 3.000 CPU sec, 3.519 real sec... [M::mem_process_seqs] Processed 749078 reads in 116.537 CPU sec, 4.027 real sec sorting block 40, #reads = 1114331 mem = 536881909 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 301723, 24, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 688, 1933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5367) [M::mem_pestat] mean and std.dev: (930.24, 1046.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (174.34, 72.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (246, 977, 2143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5937) [M::mem_pestat] mean and std.dev: (1106.57, 1061.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 426, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1586) [M::mem_pestat] mean and std.dev: (415.39, 239.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 748218 sequences (100000271 bp) in 3.345 CPU sec, 3.778 real sec... [M::mem_process_seqs] Processed 747208 reads in 119.499 CPU sec, 4.197 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 301553, 23, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 310, 1256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3440) [M::mem_pestat] mean and std.dev: (692.98, 737.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.59, 72.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (172, 203, 1207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3277) [M::mem_pestat] mean and std.dev: (468.57, 555.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 378, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2694) [M::mem_pestat] mean and std.dev: (452.14, 375.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747548 sequences (100000135 bp) in 3.011 CPU sec, 3.417 real sec... [M::mem_process_seqs] Processed 748218 reads in 116.415 CPU sec, 4.063 real sec sorting block 41, #reads = 1114111 mem = 536878078 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 301086, 20, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 285, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1412) [M::mem_pestat] mean and std.dev: (357.97, 236.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (174.07, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (393, 1513, 2227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5895) [M::mem_pestat] mean and std.dev: (1489.00, 1312.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7729) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 258, 1069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2927) [M::mem_pestat] mean and std.dev: (453.82, 561.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747042 sequences (100000056 bp) in 3.437 CPU sec, 3.820 real sec... [M::mem_process_seqs] Processed 747548 reads in 111.708 CPU sec, 3.994 real sec sorting block 42, #reads = 1114659 mem = 536878500 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 300885, 26, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 412, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2370) [M::mem_pestat] mean and std.dev: (576.67, 463.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.74, 73.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 490, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2097) [M::mem_pestat] mean and std.dev: (444.22, 275.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 349, 1630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4560) [M::mem_pestat] mean and std.dev: (724.50, 924.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747032 sequences (100000041 bp) in 3.210 CPU sec, 3.556 real sec... [M::mem_process_seqs] Processed 747042 reads in 116.004 CPU sec, 3.997 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 301152, 18, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 256, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1600) [M::mem_pestat] mean and std.dev: (302.06, 307.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.76, 73.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 533, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435) [M::mem_pestat] mean and std.dev: (452.06, 284.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 459, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2511) [M::mem_pestat] mean and std.dev: (461.50, 297.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 746492 sequences (100000140 bp) in 3.081 CPU sec, 3.416 real sec... [M::mem_process_seqs] Processed 747032 reads in 109.612 CPU sec, 3.781 real sec sorting block 43, #reads = 1114122 mem = 536886857 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 300587, 23, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 373, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143) [M::mem_pestat] mean and std.dev: (353.00, 212.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.42, 74.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (113, 260, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1481) [M::mem_pestat] mean and std.dev: (321.57, 284.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 213, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1707) [M::mem_pestat] mean and std.dev: (320.88, 248.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 745988 sequences (100000133 bp) in 3.360 CPU sec, 3.686 real sec... [M::mem_process_seqs] Processed 746492 reads in 113.910 CPU sec, 4.145 real sec sorting block 44, #reads = 1111789 mem = 536879505 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 301104, 27, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 312, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2539) [M::mem_pestat] mean and std.dev: (491.57, 407.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.84, 74.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 445, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5044) [M::mem_pestat] mean and std.dev: (880.59, 797.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 362, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3030) [M::mem_pestat] mean and std.dev: (585.20, 546.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 746280 sequences (100000002 bp) in 3.209 CPU sec, 3.670 real sec... [M::mem_process_seqs] Processed 745988 reads in 109.836 CPU sec, 3.925 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 300382, 17, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 242, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3016) [M::mem_pestat] mean and std.dev: (513.78, 533.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (175.64, 74.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 251, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1604) [M::mem_pestat] mean and std.dev: (345.65, 296.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2112) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 387, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1590) [M::mem_pestat] mean and std.dev: (417.05, 264.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 45, #reads = 1113229 mem = 536879587 peak = 786268160 [M::process] read 755410 sequences (100000268 bp) in 3.307 CPU sec, 3.699 real sec... [M::mem_process_seqs] Processed 746280 reads in 112.859 CPU sec, 4.042 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 303529, 30, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 399, 1375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3843) [M::mem_pestat] mean and std.dev: (775.41, 796.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (168.11, 69.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (147, 340, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2676) [M::mem_pestat] mean and std.dev: (575.72, 551.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3519) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 457, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177) [M::mem_pestat] mean and std.dev: (434.63, 284.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 756388 sequences (100000196 bp) in 3.187 CPU sec, 3.551 real sec... [M::mem_process_seqs] Processed 755410 reads in 111.213 CPU sec, 3.837 real sec sorting block 46, #reads = 1112339 mem = 536876222 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 303972, 27, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 336, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2637) [M::mem_pestat] mean and std.dev: (433.94, 375.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (167.80, 69.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 709, 1459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3935) [M::mem_pestat] mean and std.dev: (1054.96, 1120.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 362, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1630) [M::mem_pestat] mean and std.dev: (421.47, 336.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 756490 sequences (100000084 bp) in 2.831 CPU sec, 3.147 real sec... [M::mem_process_seqs] Processed 756388 reads in 110.353 CPU sec, 3.834 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 304010, 20, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 293, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2493) [M::mem_pestat] mean and std.dev: (457.25, 484.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (167.90, 69.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 429, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2969) [M::mem_pestat] mean and std.dev: (535.95, 585.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3942) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 231, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (236.87, 77.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754964 sequences (100000197 bp) in 3.409 CPU sec, 3.783 real sec... [M::mem_process_seqs] Processed 756490 reads in 108.948 CPU sec, 3.913 real sec sorting block 47, #reads = 1114100 mem = 536880515 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 304087, 19, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 348, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (338.26, 297.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.16, 70.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 233, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2984) [M::mem_pestat] mean and std.dev: (435.82, 604.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 336, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (349.04, 158.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754966 sequences (100000077 bp) in 3.422 CPU sec, 3.883 real sec... [M::mem_process_seqs] Processed 754964 reads in 112.250 CPU sec, 4.054 real sec sorting block 48, #reads = 1119863 mem = 536879232 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 303683, 20, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 625, 1208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3168) [M::mem_pestat] mean and std.dev: (705.83, 567.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4148) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (169.10, 70.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 894, 2457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7015) [M::mem_pestat] mean and std.dev: (1467.95, 1494.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9294) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 534, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3201) [M::mem_pestat] mean and std.dev: (780.29, 775.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 753604 sequences (100000148 bp) in 3.232 CPU sec, 3.607 real sec... [M::mem_process_seqs] Processed 754966 reads in 110.054 CPU sec, 3.871 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 303069, 15, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 240, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1947) [M::mem_pestat] mean and std.dev: (375.58, 388.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (170.30, 71.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (162, 333, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1620) [M::mem_pestat] mean and std.dev: (280.00, 207.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 368, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2011) [M::mem_pestat] mean and std.dev: (414.67, 345.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2613) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 49, #reads = 1120280 mem = 536881875 peak = 786268160 [M::process] read 752636 sequences (100000085 bp) in 3.213 CPU sec, 3.565 real sec... [M::mem_process_seqs] Processed 753604 reads in 110.130 CPU sec, 3.867 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 303117, 23, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 190, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (385.06, 446.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.04, 71.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 680, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2221) [M::mem_pestat] mean and std.dev: (521.26, 326.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2843) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 221, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (281.80, 184.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 752400 sequences (100000220 bp) in 3.232 CPU sec, 3.632 real sec... [M::mem_process_seqs] Processed 752636 reads in 107.542 CPU sec, 3.808 real sec sorting block 50, #reads = 1118857 mem = 536881658 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 303128, 28, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 358, 1581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4419) [M::mem_pestat] mean and std.dev: (908.92, 1099.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 158, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (171.48, 72.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 618, 1345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3687) [M::mem_pestat] mean and std.dev: (719.96, 789.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4858) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 569, 1246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302) [M::mem_pestat] mean and std.dev: (727.94, 588.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751330 sequences (100000056 bp) in 3.099 CPU sec, 3.532 real sec... [M::mem_process_seqs] Processed 752400 reads in 113.376 CPU sec, 3.994 real sec sorting block 51, #reads = 1117793 mem = 536884132 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 302898, 19, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 404, 859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2161) [M::mem_pestat] mean and std.dev: (390.53, 303.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.71, 72.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (126, 550, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3570) [M::mem_pestat] mean and std.dev: (842.79, 903.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4718) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 320, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3933) [M::mem_pestat] mean and std.dev: (634.30, 795.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751512 sequences (100000299 bp) in 3.020 CPU sec, 3.384 real sec... [M::mem_process_seqs] Processed 751330 reads in 107.162 CPU sec, 3.710 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 302874, 19, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 248, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2616) [M::mem_pestat] mean and std.dev: (380.75, 380.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.60, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 808, 1753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4609) [M::mem_pestat] mean and std.dev: (961.42, 735.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 209, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (278.68, 216.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750698 sequences (100000232 bp) in 3.411 CPU sec, 3.754 real sec... [M::mem_process_seqs] Processed 751512 reads in 112.577 CPU sec, 3.953 real sec sorting block 52, #reads = 1117760 mem = 536878493 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 301910, 25, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 298, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2021) [M::mem_pestat] mean and std.dev: (456.00, 386.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.92, 72.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (172, 480, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3388) [M::mem_pestat] mean and std.dev: (690.96, 698.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4460) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 455, 1648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4634) [M::mem_pestat] mean and std.dev: (764.78, 801.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750452 sequences (100000225 bp) in 2.895 CPU sec, 3.254 real sec... [M::mem_process_seqs] Processed 750698 reads in 107.775 CPU sec, 3.727 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 301884, 24, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 338, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2414) [M::mem_pestat] mean and std.dev: (576.21, 578.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (172.05, 72.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (99, 243, 2575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7527) [M::mem_pestat] mean and std.dev: (1422.91, 1905.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 347, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (348.78, 225.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 53, #reads = 1117748 mem = 536886130 peak = 786268160 [M::process] read 750082 sequences (100000145 bp) in 3.361 CPU sec, 3.816 real sec... [M::mem_process_seqs] Processed 750452 reads in 116.043 CPU sec, 4.013 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 302109, 30, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 198, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (192.00, 73.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.08, 71.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 520, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2326) [M::mem_pestat] mean and std.dev: (599.89, 500.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 266, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456) [M::mem_pestat] mean and std.dev: (354.65, 223.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749442 sequences (100000199 bp) in 2.839 CPU sec, 3.127 real sec... [M::mem_process_seqs] Processed 750082 reads in 107.387 CPU sec, 3.707 real sec sorting block 54, #reads = 1115112 mem = 536878687 peak = 786268160 [M::process] read 750360 sequences (100000010 bp) in 2.772 CPU sec, 3.047 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 301636, 36, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 789, 1328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3596) [M::mem_pestat] mean and std.dev: (928.39, 996.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (171.93, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 306, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2426) [M::mem_pestat] mean and std.dev: (496.23, 497.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3188) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 272, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2816) [M::mem_pestat] mean and std.dev: (550.23, 628.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749442 reads in 112.863 CPU sec, 3.850 real sec sorting block 55, #reads = 1115131 mem = 536879977 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 302440, 20, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 485, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2906) [M::mem_pestat] mean and std.dev: (607.09, 693.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.54, 71.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (316, 588, 1890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5038) [M::mem_pestat] mean and std.dev: (837.53, 862.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6612) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 235, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2314) [M::mem_pestat] mean and std.dev: (443.20, 448.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749926 sequences (100000260 bp) in 2.717 CPU sec, 2.984 real sec... [M::mem_process_seqs] Processed 750360 reads in 107.709 CPU sec, 3.732 real sec [M::process] read 749644 sequences (100000074 bp) in 2.884 CPU sec, 3.213 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 301929, 22, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 323, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3202) [M::mem_pestat] mean and std.dev: (585.88, 612.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (171.70, 71.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 583, 1919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5497) [M::mem_pestat] mean and std.dev: (799.63, 1000.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 405, 1099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2949) [M::mem_pestat] mean and std.dev: (598.79, 627.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3874) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 56, #reads = 1114098 mem = 536879816 peak = 786268160 [M::mem_process_seqs] Processed 749926 reads in 114.631 CPU sec, 3.900 real sec [M::process] read 748976 sequences (100000124 bp) in 2.623 CPU sec, 2.852 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 302138, 23, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 393, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3405) [M::mem_pestat] mean and std.dev: (710.83, 761.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (171.83, 71.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 508, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1707) [M::mem_pestat] mean and std.dev: (407.50, 300.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 394, 834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2104) [M::mem_pestat] mean and std.dev: (469.63, 371.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749644 reads in 108.569 CPU sec, 3.728 real sec sorting block 57, #reads = 1115347 mem = 536878887 peak = 786268160 [M::process] read 748320 sequences (100000164 bp) in 2.966 CPU sec, 3.228 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 301087, 33, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 326, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3084) [M::mem_pestat] mean and std.dev: (501.68, 505.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.54, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 343, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3638) [M::mem_pestat] mean and std.dev: (625.84, 765.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 261, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435) [M::mem_pestat] mean and std.dev: (373.95, 281.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 748976 reads in 113.688 CPU sec, 3.830 real sec [M::process] read 747958 sequences (100000113 bp) in 2.751 CPU sec, 2.984 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 301433, 23, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 328, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2070) [M::mem_pestat] mean and std.dev: (406.67, 331.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.35, 72.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 606, 2658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7834) [M::mem_pestat] mean and std.dev: (1396.78, 1738.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 298, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2725) [M::mem_pestat] mean and std.dev: (632.38, 696.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 58, #reads = 1115732 mem = 536878631 peak = 786268160 [M::mem_process_seqs] Processed 748320 reads in 108.770 CPU sec, 3.736 real sec [M::process] read 748148 sequences (100000213 bp) in 2.578 CPU sec, 2.818 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 301181, 26, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 478, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3142) [M::mem_pestat] mean and std.dev: (578.95, 667.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.53, 72.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (243, 673, 986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2472) [M::mem_pestat] mean and std.dev: (588.27, 426.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 194, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1415) [M::mem_pestat] mean and std.dev: (236.50, 189.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 59, #reads = 1112962 mem = 536880211 peak = 786268160 [M::process] read 747798 sequences (100000085 bp) in 2.779 CPU sec, 3.115 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 301576, 22, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 260, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2912) [M::mem_pestat] mean and std.dev: (501.74, 518.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.62, 72.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (125, 967, 3317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9701) [M::mem_pestat] mean and std.dev: (1850.23, 2422.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 267, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985) [M::mem_pestat] mean and std.dev: (375.91, 410.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747958 reads in 202.269 CPU sec, 6.699 real sec [M::mem_process_seqs] Processed 748148 reads in 134.489 CPU sec, 4.494 real sec [M::process] read 747050 sequences (100000100 bp) in 2.814 CPU sec, 3.192 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 301569, 24, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 587, 1334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3592) [M::mem_pestat] mean and std.dev: (833.00, 745.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (174.18, 73.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 640, 1663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4489) [M::mem_pestat] mean and std.dev: (1025.67, 1045.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5902) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 342, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4132) [M::mem_pestat] mean and std.dev: (676.08, 762.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5442) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 60, #reads = 1114580 mem = 536880569 peak = 786268160 [M::mem_process_seqs] Processed 747798 reads in 110.723 CPU sec, 3.802 real sec [M::process] read 747220 sequences (100000135 bp) in 2.762 CPU sec, 3.103 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 301367, 23, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 278, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3406) [M::mem_pestat] mean and std.dev: (569.63, 689.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (174.31, 72.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (362, 946, 2096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5564) [M::mem_pestat] mean and std.dev: (1280.55, 1208.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 378, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3194) [M::mem_pestat] mean and std.dev: (715.79, 814.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747050 reads in 111.345 CPU sec, 3.802 real sec sorting block 61, #reads = 1113084 mem = 536881223 peak = 786268160 [M::process] read 760884 sequences (100000142 bp) in 2.594 CPU sec, 2.901 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 301058, 21, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 372, 1656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4552) [M::mem_pestat] mean and std.dev: (930.30, 1080.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.58, 73.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 583, 1367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3527) [M::mem_pestat] mean and std.dev: (794.40, 712.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4607) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 294, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1922) [M::mem_pestat] mean and std.dev: (444.11, 368.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 305875, 27, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 463, 2123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6015) [M::mem_pestat] mean and std.dev: (1034.97, 1237.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 152, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (163.00, 65.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (219, 371, 1775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4887) [M::mem_pestat] mean and std.dev: (1091.58, 1367.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 235, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3148) [M::mem_pestat] mean and std.dev: (397.67, 472.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 761074 sequences (100000266 bp) in 2.885 CPU sec, 3.139 real sec... sorting block 62, #reads = 1112154 mem = 536883386 peak = 786268160 [M::mem_process_seqs] Processed 747220 reads in 206.495 CPU sec, 6.858 real sec [M::mem_process_seqs] Processed 760884 reads in 142.253 CPU sec, 4.708 real sec [M::process] read 759684 sequences (100000147 bp) in 2.438 CPU sec, 2.682 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 305936, 17, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 355, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1366) [M::mem_pestat] mean and std.dev: (366.26, 294.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (162.56, 65.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (468, 909, 2400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6264) [M::mem_pestat] mean and std.dev: (1109.94, 1041.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 370, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1814) [M::mem_pestat] mean and std.dev: (510.61, 422.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2346) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 63, #reads = 1111045 mem = 536882099 peak = 786268160 [M::process] read 758196 sequences (100000130 bp) in 2.772 CPU sec, 3.110 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 305190, 30, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 457, 1826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5212) [M::mem_pestat] mean and std.dev: (867.10, 1096.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 152, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (163.86, 66.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 507, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4067) [M::mem_pestat] mean and std.dev: (687.24, 737.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 650, 1824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5056) [M::mem_pestat] mean and std.dev: (949.34, 971.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6672) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 761074 reads in 206.812 CPU sec, 6.738 real sec [M::mem_process_seqs] Processed 759684 reads in 140.677 CPU sec, 4.580 real sec [M::process] read 757834 sequences (100000022 bp) in 2.759 CPU sec, 2.955 real sec... sorting block 64, #reads = 1120043 mem = 536880093 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305474, 15, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 283, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1786) [M::mem_pestat] mean and std.dev: (471.05, 487.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (164.81, 66.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (90, 169, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790) [M::mem_pestat] mean and std.dev: (485.93, 503.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3690) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 328, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1677) [M::mem_pestat] mean and std.dev: (350.95, 236.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 757830 sequences (100000125 bp) in 2.642 CPU sec, 3.109 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 305318, 21, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 275, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (270.94, 142.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (164.88, 66.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (268, 953, 1929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5251) [M::mem_pestat] mean and std.dev: (1049.57, 802.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 248, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394) [M::mem_pestat] mean and std.dev: (297.36, 246.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 758196 reads in 201.469 CPU sec, 6.620 real sec [M::mem_process_seqs] Processed 757834 reads in 134.436 CPU sec, 4.404 real sec sorting block 65, #reads = 1119209 mem = 536878545 peak = 786268160 [M::process] read 756886 sequences (100000281 bp) in 2.772 CPU sec, 2.971 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 304739, 22, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 322, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1621) [M::mem_pestat] mean and std.dev: (362.55, 342.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (164.41, 66.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (383, 684, 1366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3332) [M::mem_pestat] mean and std.dev: (819.05, 711.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 336, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1919) [M::mem_pestat] mean and std.dev: (404.50, 388.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 66, #reads = 1120202 mem = 536885808 peak = 786268160 [M::process] read 756024 sequences (100000046 bp) in 2.691 CPU sec, 2.987 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 304539, 26, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 356, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3116) [M::mem_pestat] mean and std.dev: (526.75, 614.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (165.92, 67.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (324, 590, 1425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3627) [M::mem_pestat] mean and std.dev: (793.75, 634.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 351, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1705) [M::mem_pestat] mean and std.dev: (454.61, 365.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 757830 reads in 202.584 CPU sec, 6.637 real sec [M::mem_process_seqs] Processed 756886 reads in 134.353 CPU sec, 4.422 real sec [M::process] read 754648 sequences (100000201 bp) in 2.310 CPU sec, 2.482 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 304253, 17, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 445, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2050) [M::mem_pestat] mean and std.dev: (476.88, 390.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (166.56, 68.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (133, 394, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3640) [M::mem_pestat] mean and std.dev: (627.29, 620.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4809) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 232, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1497) [M::mem_pestat] mean and std.dev: (367.15, 302.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1946) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 67, #reads = 1118455 mem = 536879215 peak = 786268160 [M::process] read 754730 sequences (100000289 bp) in 2.756 CPU sec, 3.153 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 303870, 24, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 526, 1316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3614) [M::mem_pestat] mean and std.dev: (745.86, 774.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (167.54, 68.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 533, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2674) [M::mem_pestat] mean and std.dev: (434.18, 395.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 836, 2441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6865) [M::mem_pestat] mean and std.dev: (1493.94, 1570.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 756024 reads in 199.757 CPU sec, 6.602 real sec [M::mem_process_seqs] Processed 754648 reads in 134.106 CPU sec, 4.445 real sec [M::process] read 754860 sequences (100000023 bp) in 2.508 CPU sec, 2.648 real sec... sorting block 68, #reads = 1119112 mem = 536879546 peak = 786268160 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 303515, 17, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 301, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3454) [M::mem_pestat] mean and std.dev: (514.59, 551.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (167.71, 68.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 205, 2150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6120) [M::mem_pestat] mean and std.dev: (745.53, 1284.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8105) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 463, 1817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5121) [M::mem_pestat] mean and std.dev: (846.53, 880.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754656 sequences (100000008 bp) in 3.002 CPU sec, 3.469 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 303526, 20, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 298, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478) [M::mem_pestat] mean and std.dev: (367.78, 303.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (167.48, 68.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 661, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3032) [M::mem_pestat] mean and std.dev: (435.31, 312.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 207, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2161) [M::mem_pestat] mean and std.dev: (349.77, 318.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754730 reads in 202.998 CPU sec, 6.583 real sec sorting block 69, #reads = 1118267 mem = 536879928 peak = 786268160 [M::mem_process_seqs] Processed 754860 reads in 137.974 CPU sec, 4.460 real sec [M::process] read 752808 sequences (100000265 bp) in 2.338 CPU sec, 2.525 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 303369, 11, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 187, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (267.26, 167.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 155, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (166.98, 67.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (215, 751, 1639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4487) [M::mem_pestat] mean and std.dev: (953.30, 1207.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5911) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 256, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (264.76, 141.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 70, #reads = 1116495 mem = 536880000 peak = 823029760 [M::process] read 753646 sequences (100000166 bp) in 2.937 CPU sec, 3.420 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 303192, 29, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 366, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1825) [M::mem_pestat] mean and std.dev: (490.23, 464.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (169.20, 69.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (123, 246, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1923) [M::mem_pestat] mean and std.dev: (380.30, 365.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 473, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3598) [M::mem_pestat] mean and std.dev: (786.34, 820.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754656 reads in 205.382 CPU sec, 6.760 real sec [M::mem_process_seqs] Processed 752808 reads in 137.390 CPU sec, 4.484 real sec [M::process] read 752166 sequences (100000215 bp) in 2.568 CPU sec, 2.750 real sec... sorting block 71, #reads = 1116829 mem = 536881182 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 303524, 21, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 227, 1382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3878) [M::mem_pestat] mean and std.dev: (736.19, 999.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.34, 69.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (87, 375, 1784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5178) [M::mem_pestat] mean and std.dev: (954.38, 1145.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 346, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3415) [M::mem_pestat] mean and std.dev: (716.70, 628.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750296 sequences (100000095 bp) in 2.649 CPU sec, 3.074 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 303102, 25, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 211, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3922) [M::mem_pestat] mean and std.dev: (630.86, 702.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (170.03, 70.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (154, 657, 2342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6718) [M::mem_pestat] mean and std.dev: (783.33, 1036.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8906) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 234, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (277.90, 202.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 753646 reads in 200.536 CPU sec, 6.563 real sec [M::mem_process_seqs] Processed 752166 reads in 135.562 CPU sec, 4.412 real sec sorting block 72, #reads = 1116037 mem = 536879111 peak = 823029760 [M::process] read 749120 sequences (100000017 bp) in 2.585 CPU sec, 2.798 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 302688, 24, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 573, 1495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4111) [M::mem_pestat] mean and std.dev: (858.24, 827.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (172.21, 72.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (343, 591, 2355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6379) [M::mem_pestat] mean and std.dev: (1205.91, 1547.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 312, 1214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3200) [M::mem_pestat] mean and std.dev: (699.21, 650.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747634 sequences (100000008 bp) in 2.700 CPU sec, 3.052 real sec... sorting block 73, #reads = 1115705 mem = 536880231 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 301803, 24, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 218, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1823) [M::mem_pestat] mean and std.dev: (402.21, 420.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.32, 72.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (246, 352, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1734) [M::mem_pestat] mean and std.dev: (347.74, 212.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 235, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2225) [M::mem_pestat] mean and std.dev: (385.48, 394.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 750296 reads in 203.932 CPU sec, 6.629 real sec [M::mem_process_seqs] Processed 749120 reads in 137.809 CPU sec, 4.425 real sec [M::process] read 747114 sequences (100000037 bp) in 2.880 CPU sec, 3.084 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 301344, 17, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 334, 1826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5110) [M::mem_pestat] mean and std.dev: (989.85, 1133.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (174.35, 73.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 478, 1572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4170) [M::mem_pestat] mean and std.dev: (763.81, 650.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 261, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1202) [M::mem_pestat] mean and std.dev: (354.23, 292.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 74, #reads = 1114033 mem = 536878375 peak = 823029760 [M::process] read 746588 sequences (100000210 bp) in 2.855 CPU sec, 3.049 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 300752, 26, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 280, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3376) [M::mem_pestat] mean and std.dev: (787.57, 875.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.64, 73.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 507, 1558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4288) [M::mem_pestat] mean and std.dev: (861.25, 1030.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 574, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4130) [M::mem_pestat] mean and std.dev: (876.89, 969.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747634 reads in 200.309 CPU sec, 6.427 real sec [M::mem_process_seqs] Processed 747114 reads in 137.637 CPU sec, 4.363 real sec [M::process] read 746938 sequences (100000138 bp) in 3.613 CPU sec, 3.861 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 300257, 23, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 350, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4403) [M::mem_pestat] mean and std.dev: (823.37, 914.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5825) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 75, #reads = 1110866 mem = 536877389 peak = 823029760 [M::mem_pestat] (25, 50, 75) percentile: (119, 162, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (174.65, 73.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (449, 894, 5390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15272) [M::mem_pestat] mean and std.dev: (2228.57, 2711.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20213) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 924, 1273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3351) [M::mem_pestat] mean and std.dev: (863.04, 674.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 746588 reads in 115.081 CPU sec, 3.850 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 300519, 30, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 597, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3078) [M::mem_pestat] mean and std.dev: (719.97, 605.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (174.33, 73.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 358, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2236) [M::mem_pestat] mean and std.dev: (468.56, 488.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 301, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2530) [M::mem_pestat] mean and std.dev: (491.82, 508.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 747194 sequences (100000197 bp) in 4.182 CPU sec, 4.634 real sec... [M::mem_process_seqs] Processed 746938 reads in 118.401 CPU sec, 4.098 real sec sorting block 76, #reads = 1109987 mem = 536878540 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 300548, 32, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 227, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (248.27, 154.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (119, 161, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (174.19, 73.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (150, 360, 2073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5919) [M::mem_pestat] mean and std.dev: (1168.03, 1560.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7842) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 306, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2563) [M::mem_pestat] mean and std.dev: (490.14, 459.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 747194 reads in 118.596 CPU sec, 3.990 real sec [M::process] read 762614 sequences (100000190 bp) in 5.105 CPU sec, 5.540 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 306067, 25, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 689, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4010) [M::mem_pestat] mean and std.dev: (760.48, 743.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (161.93, 64.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 607, 2132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5996) [M::mem_pestat] mean and std.dev: (897.59, 1064.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7928) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 213, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623) [M::mem_pestat] mean and std.dev: (433.29, 380.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 762614 reads in 129.239 CPU sec, 4.375 real sec sorting block 77, #reads = 1110592 mem = 536881068 peak = 823029760 [M::process] read 762624 sequences (100000262 bp) in 4.782 CPU sec, 5.462 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 306201, 27, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 395, 726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1884) [M::mem_pestat] mean and std.dev: (477.40, 434.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (161.82, 64.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 329, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1796) [M::mem_pestat] mean and std.dev: (428.48, 390.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 362, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1810) [M::mem_pestat] mean and std.dev: (454.57, 456.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2351) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 762624 reads in 126.967 CPU sec, 4.509 real sec [M::process] read 762196 sequences (100000210 bp) in 4.314 CPU sec, 4.752 real sec... sorting block 78, #reads = 1108324 mem = 536879430 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 306169, 25, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 198, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1725) [M::mem_pestat] mean and std.dev: (238.25, 223.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 151, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (162.39, 65.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 631, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3297) [M::mem_pestat] mean and std.dev: (675.74, 588.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 871, 1861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5021) [M::mem_pestat] mean and std.dev: (955.90, 1109.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6601) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 759192 sequences (100000124 bp) in 3.858 CPU sec, 4.250 real sec... [M::mem_process_seqs] Processed 762196 reads in 130.645 CPU sec, 4.415 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 305088, 24, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 278, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2506) [M::mem_pestat] mean and std.dev: (480.38, 483.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (164.42, 66.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 428, 2196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6456) [M::mem_pestat] mean and std.dev: (1035.50, 1322.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 244, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026) [M::mem_pestat] mean and std.dev: (273.74, 142.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1311) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 79, #reads = 1116994 mem = 536880429 peak = 823029760 [M::mem_process_seqs] Processed 759192 reads in 123.694 CPU sec, 4.153 real sec [M::process] read 758902 sequences (100000132 bp) in 4.189 CPU sec, 4.539 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 305585, 32, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 185, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1836) [M::mem_pestat] mean and std.dev: (285.64, 226.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (164.46, 66.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 1100, 2745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7459) [M::mem_pestat] mean and std.dev: (1567.03, 1578.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 192, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1187) [M::mem_pestat] mean and std.dev: (261.97, 191.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 758902 reads in 119.620 CPU sec, 4.040 real sec [M::process] read 757788 sequences (100000017 bp) in 3.750 CPU sec, 4.102 real sec... sorting block 80, #reads = 1113601 mem = 536880105 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 304499, 23, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 408, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2881) [M::mem_pestat] mean and std.dev: (651.58, 651.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (165.40, 67.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (155, 383, 1427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3971) [M::mem_pestat] mean and std.dev: (682.48, 797.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 372, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3061) [M::mem_pestat] mean and std.dev: (712.48, 762.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 756636 sequences (100000027 bp) in 3.511 CPU sec, 3.918 real sec... [M::mem_process_seqs] Processed 757788 reads in 124.828 CPU sec, 4.186 real sec sorting block 81, #reads = 1113361 mem = 536888277 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 304433, 16, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 274, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024) [M::mem_pestat] mean and std.dev: (387.81, 381.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (166.53, 68.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 462, 1052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2870) [M::mem_pestat] mean and std.dev: (565.73, 708.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3779) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 558, 1411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3789) [M::mem_pestat] mean and std.dev: (758.21, 814.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 756636 reads in 113.702 CPU sec, 3.873 real sec [M::process] read 755748 sequences (100000037 bp) in 3.738 CPU sec, 3.980 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 304429, 23, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 325, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2508) [M::mem_pestat] mean and std.dev: (409.42, 422.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (167.18, 68.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 772, 1528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4456) [M::mem_pestat] mean and std.dev: (831.74, 817.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5920) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 505, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2737) [M::mem_pestat] mean and std.dev: (634.54, 663.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755748 reads in 111.177 CPU sec, 3.856 real sec sorting block 82, #reads = 1113348 mem = 536877147 peak = 823029760 [M::process] read 755602 sequences (100000275 bp) in 5.207 CPU sec, 5.707 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 303917, 23, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 392, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2110) [M::mem_pestat] mean and std.dev: (400.38, 321.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (167.32, 68.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (177, 752, 2223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6315) [M::mem_pestat] mean and std.dev: (1081.67, 1186.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 257, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2756) [M::mem_pestat] mean and std.dev: (438.52, 446.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755602 reads in 133.058 CPU sec, 4.438 real sec [M::process] read 755312 sequences (100000251 bp) in 4.266 CPU sec, 4.750 real sec... sorting block 83, #reads = 1115154 mem = 536879739 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 304470, 18, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 381, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3106) [M::mem_pestat] mean and std.dev: (689.49, 605.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (167.82, 69.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 259, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2116) [M::mem_pestat] mean and std.dev: (479.00, 481.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 229, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (251.97, 159.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755312 reads in 122.005 CPU sec, 4.263 real sec [M::process] read 754342 sequences (100000115 bp) in 4.584 CPU sec, 4.937 real sec... sorting block 84, #reads = 1111455 mem = 536882471 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 303717, 25, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 400, 1118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2986) [M::mem_pestat] mean and std.dev: (594.00, 545.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.27, 69.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (222, 977, 2984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8508) [M::mem_pestat] mean and std.dev: (1858.72, 2200.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 353, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1497) [M::mem_pestat] mean and std.dev: (385.56, 237.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 753194 sequences (100000240 bp) in 3.306 CPU sec, 3.545 real sec... [M::mem_process_seqs] Processed 754342 reads in 113.108 CPU sec, 3.833 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 303278, 25, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 491, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3813) [M::mem_pestat] mean and std.dev: (796.08, 788.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (168.84, 69.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (230, 488, 1498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4034) [M::mem_pestat] mean and std.dev: (700.59, 697.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 282, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2374) [M::mem_pestat] mean and std.dev: (404.14, 404.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 753098 sequences (100000047 bp) in 3.545 CPU sec, 3.852 real sec... [M::mem_process_seqs] Processed 753194 reads in 111.499 CPU sec, 3.682 real sec sorting block 85, #reads = 1115074 mem = 536878502 peak = 823029760 [M::process] read 752912 sequences (100000271 bp) in 2.747 CPU sec, 3.034 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 303093, 24, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 279, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1402) [M::mem_pestat] mean and std.dev: (334.31, 283.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (169.31, 70.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 359, 2643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7561) [M::mem_pestat] mean and std.dev: (1227.61, 1434.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 202, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2436) [M::mem_pestat] mean and std.dev: (483.97, 539.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 86, #reads = 1113740 mem = 536882656 peak = 823029760 [M::process] read 752078 sequences (100000169 bp) in 2.341 CPU sec, 2.559 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 303232, 27, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 444, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2230) [M::mem_pestat] mean and std.dev: (474.12, 443.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (169.29, 70.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 420, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4079) [M::mem_pestat] mean and std.dev: (695.79, 901.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 246, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2226) [M::mem_pestat] mean and std.dev: (424.68, 464.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 753098 reads in 206.539 CPU sec, 6.611 real sec [M::mem_process_seqs] Processed 752912 reads in 136.363 CPU sec, 4.330 real sec [M::process] read 750748 sequences (100000185 bp) in 2.263 CPU sec, 2.360 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 303163, 34, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 301, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1688) [M::mem_pestat] mean and std.dev: (357.89, 285.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (169.66, 70.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 213, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218) [M::mem_pestat] mean and std.dev: (237.22, 193.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 386, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2116) [M::mem_pestat] mean and std.dev: (473.06, 429.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 87, #reads = 1112477 mem = 536880750 peak = 823029760 [M::process] read 748754 sequences (100000044 bp) in 2.885 CPU sec, 3.068 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 302575, 18, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 306, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1930) [M::mem_pestat] mean and std.dev: (431.07, 377.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (171.70, 72.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 392, 1777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4921) [M::mem_pestat] mean and std.dev: (761.82, 861.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6493) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 307, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2309) [M::mem_pestat] mean and std.dev: (508.07, 541.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 752078 reads in 195.801 CPU sec, 6.259 real sec [M::process] read 748916 sequences (100000172 bp) in 2.145 CPU sec, 2.172 real sec... [M::mem_process_seqs] Processed 750748 reads in 130.511 CPU sec, 4.305 real sec sorting block 88, #reads = 1115580 mem = 536877501 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 302200, 16, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 229, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213) [M::mem_pestat] mean and std.dev: (363.66, 290.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (173.17, 72.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (440, 977, 2507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6641) [M::mem_pestat] mean and std.dev: (1440.75, 1266.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 303, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1505) [M::mem_pestat] mean and std.dev: (300.26, 223.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 748480 sequences (100000077 bp) in 3.784 CPU sec, 4.049 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 301784, 20, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 213, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1848) [M::mem_pestat] mean and std.dev: (354.18, 332.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.55, 72.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 548, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2442) [M::mem_pestat] mean and std.dev: (564.11, 447.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3191) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 188, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1598) [M::mem_pestat] mean and std.dev: (352.62, 367.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 748754 reads in 202.186 CPU sec, 6.642 real sec sorting block 89, #reads = 1114340 mem = 536878836 peak = 823029760 [M::process] read 748496 sequences (100000151 bp) in 4.399 CPU sec, 4.615 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 301109, 24, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 271, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2345) [M::mem_pestat] mean and std.dev: (498.76, 436.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3069) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.48, 71.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 634, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2183) [M::mem_pestat] mean and std.dev: (585.39, 595.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2965) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 306, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3189) [M::mem_pestat] mean and std.dev: (671.38, 688.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 748480 reads in 114.311 CPU sec, 3.685 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 301395, 17, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 321, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1556) [M::mem_pestat] mean and std.dev: (417.82, 337.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 160, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.49, 71.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 246, 2242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6362) [M::mem_pestat] mean and std.dev: (866.24, 1017.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 227, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2753) [M::mem_pestat] mean and std.dev: (431.71, 394.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3617) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 748496 reads in 132.650 CPU sec, 4.333 real sec sorting block 90, #reads = 1111088 mem = 536878775 peak = 823029760 [M::mem_process_seqs] Processed 748916 reads in 358.344 CPU sec, 11.771 real sec [M::process] read 748922 sequences (100000029 bp) in 4.550 CPU sec, 4.879 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 301156, 26, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 367, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (440.14, 346.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (171.54, 70.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 519, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2485) [M::mem_pestat] mean and std.dev: (475.50, 371.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 273, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640) [M::mem_pestat] mean and std.dev: (355.80, 264.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755368 sequences (100000273 bp) in 3.432 CPU sec, 3.734 real sec... [M::mem_process_seqs] Processed 748922 reads in 120.243 CPU sec, 4.148 real sec sorting block 91, #reads = 1111312 mem = 536883965 peak = 823029760 [M::process] read 761238 sequences (100000218 bp) in 3.422 CPU sec, 3.754 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 303081, 27, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 234, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1446) [M::mem_pestat] mean and std.dev: (343.93, 284.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (166.92, 68.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (105, 378, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2139) [M::mem_pestat] mean and std.dev: (490.48, 409.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 289, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1798) [M::mem_pestat] mean and std.dev: (404.24, 354.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2341) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755368 reads in 131.061 CPU sec, 4.311 real sec sorting block 92, #reads = 1109891 mem = 536880925 peak = 823029760 [M::process] read 759974 sequences (100000112 bp) in 2.984 CPU sec, 3.140 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 305443, 16, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 260, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2604) [M::mem_pestat] mean and std.dev: (491.15, 500.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 152, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (163.05, 65.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 518, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853) [M::mem_pestat] mean and std.dev: (436.71, 252.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2376) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 414, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2036) [M::mem_pestat] mean and std.dev: (407.04, 302.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2657) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 758550 sequences (100000166 bp) in 3.042 CPU sec, 3.188 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 305729, 25, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 268, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2554) [M::mem_pestat] mean and std.dev: (416.03, 473.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (163.69, 66.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 516, 1411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3895) [M::mem_pestat] mean and std.dev: (701.75, 633.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 272, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (292.80, 178.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 761238 reads in 227.967 CPU sec, 7.288 real sec [M::mem_process_seqs] Processed 759974 reads in 135.142 CPU sec, 4.307 real sec sorting block 93, #reads = 1106607 mem = 536880906 peak = 823029760 [M::process] read 757294 sequences (100000155 bp) in 3.528 CPU sec, 3.724 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 305004, 23, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 241, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (212.30, 90.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (165.38, 67.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (106, 329, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2242) [M::mem_pestat] mean and std.dev: (445.05, 498.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 242, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (251.53, 105.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 94, #reads = 1119429 mem = 536885214 peak = 823029760 [M::process] read 756914 sequences (100000173 bp) in 2.881 CPU sec, 3.061 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 304584, 15, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 552, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2348) [M::mem_pestat] mean and std.dev: (541.69, 437.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (166.08, 67.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 408, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3088) [M::mem_pestat] mean and std.dev: (489.43, 482.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 261, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1874) [M::mem_pestat] mean and std.dev: (425.44, 382.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 758550 reads in 199.876 CPU sec, 6.385 real sec [M::mem_process_seqs] Processed 757294 reads in 135.502 CPU sec, 4.301 real sec [M::process] read 755718 sequences (100000111 bp) in 2.510 CPU sec, 2.594 real sec... sorting block 95, #reads = 1118546 mem = 536888215 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 304757, 21, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 209, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (231.83, 135.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (166.47, 68.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 298, 817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2051) [M::mem_pestat] mean and std.dev: (540.95, 500.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 392, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1826) [M::mem_pestat] mean and std.dev: (502.18, 439.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 755202 sequences (100000127 bp) in 3.298 CPU sec, 3.480 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 304602, 25, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 214, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146) [M::mem_pestat] mean and std.dev: (415.40, 380.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (167.38, 69.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (475, 540, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2074) [M::mem_pestat] mean and std.dev: (591.13, 348.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2607) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 323, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3717) [M::mem_pestat] mean and std.dev: (677.12, 814.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 756914 reads in 198.118 CPU sec, 6.318 real sec [M::mem_process_seqs] Processed 755718 reads in 134.359 CPU sec, 4.260 real sec [M::process] read 754818 sequences (100000239 bp) in 2.850 CPU sec, 2.937 real sec... sorting block 96, #reads = 1117734 mem = 536881602 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 303508, 29, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 187, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1954) [M::mem_pestat] mean and std.dev: (275.95, 227.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (167.72, 68.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (136, 569, 1851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5281) [M::mem_pestat] mean and std.dev: (921.89, 985.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 303, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (285.00, 184.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 753952 sequences (100000284 bp) in 3.447 CPU sec, 3.643 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 303332, 23, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 313, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2799) [M::mem_pestat] mean and std.dev: (668.83, 718.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (167.95, 69.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (149, 520, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3572) [M::mem_pestat] mean and std.dev: (609.95, 736.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 296, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2052) [M::mem_pestat] mean and std.dev: (406.95, 322.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 755202 reads in 196.710 CPU sec, 6.304 real sec sorting block 97, #reads = 1118117 mem = 536881893 peak = 823029760 [M::mem_process_seqs] Processed 754818 reads in 133.462 CPU sec, 4.222 real sec [M::process] read 754372 sequences (100000095 bp) in 2.961 CPU sec, 3.091 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 303571, 29, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 407, 1272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3480) [M::mem_pestat] mean and std.dev: (646.43, 632.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.46, 69.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (138, 561, 1753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4983) [M::mem_pestat] mean and std.dev: (719.85, 620.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 513, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4235) [M::mem_pestat] mean and std.dev: (729.33, 661.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 98, #reads = 1116714 mem = 536888234 peak = 823029760 [M::process] read 754024 sequences (100000094 bp) in 3.105 CPU sec, 3.294 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 303271, 20, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 321, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2246) [M::mem_pestat] mean and std.dev: (432.55, 390.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.33, 69.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 215, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4815) [M::mem_pestat] mean and std.dev: (864.00, 1195.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 267, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2480) [M::mem_pestat] mean and std.dev: (453.78, 440.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 753952 reads in 201.988 CPU sec, 6.474 real sec [M::process] read 753142 sequences (100000017 bp) in 2.245 CPU sec, 2.290 real sec... [M::mem_process_seqs] Processed 754372 reads in 135.465 CPU sec, 4.481 real sec sorting block 99, #reads = 1117230 mem = 536879150 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 303552, 14, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 320, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (301.97, 156.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (168.93, 70.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 742, 1207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3319) [M::mem_pestat] mean and std.dev: (659.00, 611.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4375) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 269, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1518) [M::mem_pestat] mean and std.dev: (390.68, 359.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 752198 sequences (100000039 bp) in 2.969 CPU sec, 3.154 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 302658, 21, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 518, 3309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9341) [M::mem_pestat] mean and std.dev: (1608.58, 1672.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (169.05, 69.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (198, 418, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3297) [M::mem_pestat] mean and std.dev: (787.90, 810.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 345, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1594) [M::mem_pestat] mean and std.dev: (372.67, 259.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754024 reads in 203.012 CPU sec, 6.678 real sec [M::process] read 751012 sequences (100000221 bp) in 3.290 CPU sec, 3.406 real sec... sorting block 100, #reads = 1115025 mem = 536878134 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 302853, 14, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 403, 2353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6753) [M::mem_pestat] mean and std.dev: (1079.38, 1276.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (170.15, 70.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (135, 533, 1474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4152) [M::mem_pestat] mean and std.dev: (598.33, 537.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5491) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 284, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2869) [M::mem_pestat] mean and std.dev: (559.36, 586.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 752198 reads in 114.111 CPU sec, 3.621 real sec [M::process] read 750304 sequences (100000082 bp) in 3.950 CPU sec, 4.088 real sec... sorting block 101, #reads = 1114976 mem = 536876131 peak = 823029760 [M::mem_process_seqs] Processed 753142 reads in 329.868 CPU sec, 10.464 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 302318, 17, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 425, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2079) [M::mem_pestat] mean and std.dev: (471.82, 400.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.07, 71.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 508, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3470) [M::mem_pestat] mean and std.dev: (884.44, 858.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 316, 1480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4068) [M::mem_pestat] mean and std.dev: (841.81, 955.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749598 sequences (100000128 bp) in 2.790 CPU sec, 3.267 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 301900, 25, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 204, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (245.69, 161.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (171.74, 71.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (253, 336, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3853) [M::mem_pestat] mean and std.dev: (621.67, 544.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5053) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 277, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3016) [M::mem_pestat] mean and std.dev: (686.55, 690.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 751012 reads in 224.991 CPU sec, 7.164 real sec [M::mem_process_seqs] Processed 750304 reads in 137.937 CPU sec, 4.394 real sec sorting block 102, #reads = 1114378 mem = 536886543 peak = 823029760 [M::process] read 749772 sequences (100000110 bp) in 2.690 CPU sec, 2.838 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 301515, 22, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 267, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1521) [M::mem_pestat] mean and std.dev: (340.27, 248.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (172.33, 72.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (514, 787, 2116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5320) [M::mem_pestat] mean and std.dev: (1269.81, 1191.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 235, 2095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5981) [M::mem_pestat] mean and std.dev: (924.12, 1227.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 103, #reads = 1113238 mem = 536879638 peak = 823029760 [M::process] read 750252 sequences (100000278 bp) in 2.775 CPU sec, 3.099 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 302407, 23, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 420, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1636) [M::mem_pestat] mean and std.dev: (436.19, 374.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (171.82, 71.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 533, 1316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3428) [M::mem_pestat] mean and std.dev: (774.45, 772.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4484) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 389, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4185) [M::mem_pestat] mean and std.dev: (800.25, 785.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749598 reads in 190.319 CPU sec, 6.059 real sec [M::process] read 749938 sequences (100000037 bp) in 2.309 CPU sec, 2.346 real sec... [M::mem_process_seqs] Processed 749772 reads in 131.933 CPU sec, 4.215 real sec sorting block 104, #reads = 1112293 mem = 536879349 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 301640, 21, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 318, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2137) [M::mem_pestat] mean and std.dev: (422.56, 380.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (171.31, 70.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 394, 1532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4162) [M::mem_pestat] mean and std.dev: (663.35, 608.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 444, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2734) [M::mem_pestat] mean and std.dev: (490.77, 425.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3585) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749500 sequences (100000129 bp) in 2.999 CPU sec, 3.173 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 302127, 19, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 201, 1380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3864) [M::mem_pestat] mean and std.dev: (541.29, 640.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (171.09, 70.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (106, 240, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276) [M::mem_pestat] mean and std.dev: (292.00, 227.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1666) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1935) [M::mem_pestat] mean and std.dev: (552.59, 519.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2632) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 749732 sequences (100000039 bp) in 2.094 CPU sec, 2.089 real sec... [M::mem_process_seqs] Processed 750252 reads in 195.336 CPU sec, 6.268 real sec [M::mem_process_seqs] Processed 749938 reads in 130.363 CPU sec, 4.231 real sec sorting block 105, #reads = 1111691 mem = 536881640 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 301640, 33, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 225, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (208.37, 121.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (171.85, 71.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 1068, 1750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4728) [M::mem_pestat] mean and std.dev: (1149.58, 1053.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 232, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1274) [M::mem_pestat] mean and std.dev: (319.43, 238.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1642) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750212 sequences (100000199 bp) in 2.751 CPU sec, 2.878 real sec... [M::process] read 764258 sequences (100000098 bp) in 1.709 CPU sec, 1.705 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 301491, 13, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 232, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2223) [M::mem_pestat] mean and std.dev: (450.18, 529.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2922) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (171.15, 70.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 405, 3035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8561) [M::mem_pestat] mean and std.dev: (1244.58, 1945.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11324) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 222, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (301.70, 260.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1766) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 749500 reads in 194.395 CPU sec, 6.297 real sec sorting block 106, #reads = 1110844 mem = 536879943 peak = 823029760 [M::process] read 764294 sequences (100000072 bp) in 2.334 CPU sec, 2.336 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 300928, 27, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 255, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861) [M::mem_pestat] mean and std.dev: (436.74, 399.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.15, 71.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 401, 1600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4312) [M::mem_pestat] mean and std.dev: (1120.33, 1193.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 313, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4006) [M::mem_pestat] mean and std.dev: (681.66, 739.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 750212 reads in 124.108 CPU sec, 4.055 real sec sorting block 107, #reads = 1105129 mem = 536880756 peak = 823029760 [M::mem_process_seqs] Processed 749732 reads in 287.130 CPU sec, 9.257 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 306624, 25, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 410, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3140) [M::mem_pestat] mean and std.dev: (665.94, 674.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 150, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.99, 64.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (246, 478, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2514) [M::mem_pestat] mean and std.dev: (640.87, 627.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 237, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3132) [M::mem_pestat] mean and std.dev: (599.46, 681.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 763084 sequences (100000083 bp) in 2.724 CPU sec, 3.067 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 306665, 25, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 385, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2581) [M::mem_pestat] mean and std.dev: (618.32, 635.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 150, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (160.94, 64.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 888, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4840) [M::mem_pestat] mean and std.dev: (1020.79, 909.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 410, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2535) [M::mem_pestat] mean and std.dev: (555.91, 461.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 761018 sequences (100000254 bp) in 1.890 CPU sec, 1.884 real sec... [M::mem_process_seqs] Processed 764258 reads in 226.543 CPU sec, 7.204 real sec sorting block 108, #reads = 1105806 mem = 536876604 peak = 823029760 [M::mem_process_seqs] Processed 764294 reads in 139.650 CPU sec, 4.433 real sec sorting block 109, #reads = 1114467 mem = 536876844 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 306472, 26, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 259, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212) [M::mem_pestat] mean and std.dev: (346.55, 278.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 150, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (161.53, 64.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 931, 1896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5218) [M::mem_pestat] mean and std.dev: (816.57, 842.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6879) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 285, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1824) [M::mem_pestat] mean and std.dev: (350.72, 294.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2383) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 759426 sequences (100000245 bp) in 3.146 CPU sec, 3.316 real sec... [M::process] read 758124 sequences (100000000 bp) in 2.268 CPU sec, 2.270 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 305576, 18, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 451, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2875) [M::mem_pestat] mean and std.dev: (537.21, 402.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (113, 152, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (163.34, 66.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (173, 351, 1543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4283) [M::mem_pestat] mean and std.dev: (914.11, 1035.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 210, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (193.24, 55.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 763084 reads in 211.379 CPU sec, 7.026 real sec [M::mem_process_seqs] Processed 761018 reads in 140.237 CPU sec, 4.572 real sec sorting block 110, #reads = 1112195 mem = 536879445 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 305279, 31, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 271, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3106) [M::mem_pestat] mean and std.dev: (588.41, 641.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 153, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (164.49, 66.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 570, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3280) [M::mem_pestat] mean and std.dev: (711.48, 762.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4324) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 255, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1227) [M::mem_pestat] mean and std.dev: (275.48, 219.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 757206 sequences (100000193 bp) in 3.314 CPU sec, 3.500 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 304298, 25, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 283, 1634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4636) [M::mem_pestat] mean and std.dev: (732.21, 881.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (114, 154, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (165.47, 67.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 557, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3962) [M::mem_pestat] mean and std.dev: (657.36, 753.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 351, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3452) [M::mem_pestat] mean and std.dev: (674.73, 624.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 759426 reads in 203.346 CPU sec, 6.586 real sec [M::process] read 756752 sequences (100000069 bp) in 2.451 CPU sec, 2.462 real sec... [M::mem_process_seqs] Processed 758124 reads in 136.948 CPU sec, 4.604 real sec sorting block 111, #reads = 1114674 mem = 536879655 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 304591, 22, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 185, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2007) [M::mem_pestat] mean and std.dev: (349.04, 357.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 154, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (166.29, 68.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 868, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4277) [M::mem_pestat] mean and std.dev: (947.86, 823.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 469, 1280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3462) [M::mem_pestat] mean and std.dev: (689.50, 659.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754994 sequences (100000083 bp) in 3.092 CPU sec, 3.289 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 304023, 15, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 345, 1642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4588) [M::mem_pestat] mean and std.dev: (785.06, 789.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (115, 155, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (166.69, 68.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (363, 844, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4371) [M::mem_pestat] mean and std.dev: (699.25, 517.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 352, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3181) [M::mem_pestat] mean and std.dev: (551.64, 555.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754872 sequences (100000060 bp) in 2.131 CPU sec, 2.135 real sec... [M::mem_process_seqs] Processed 757206 reads in 204.814 CPU sec, 6.787 real sec sorting block 112, #reads = 1114296 mem = 536882641 peak = 823029760 [M::mem_process_seqs] Processed 756752 reads in 137.070 CPU sec, 4.434 real sec sorting block 113, #reads = 1115657 mem = 536881855 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 303492, 20, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 379, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3206) [M::mem_pestat] mean and std.dev: (676.19, 700.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.22, 69.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (302, 621, 2193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5975) [M::mem_pestat] mean and std.dev: (1129.26, 1290.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 329, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1881) [M::mem_pestat] mean and std.dev: (424.85, 366.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2445) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 754116 sequences (100000135 bp) in 3.204 CPU sec, 3.349 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 303660, 14, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 372, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744) [M::mem_pestat] mean and std.dev: (370.29, 308.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.19, 69.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 521, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2917) [M::mem_pestat] mean and std.dev: (682.85, 744.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 370, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3959) [M::mem_pestat] mean and std.dev: (758.09, 879.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 752402 sequences (100000130 bp) in 2.150 CPU sec, 2.153 real sec... [M::mem_process_seqs] Processed 754994 reads in 199.358 CPU sec, 6.441 real sec [M::mem_process_seqs] Processed 754872 reads in 129.693 CPU sec, 4.240 real sec sorting block 114, #reads = 1111867 mem = 536880285 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 303954, 23, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 260, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (394.87, 380.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 156, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (168.30, 69.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 505, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2692) [M::mem_pestat] mean and std.dev: (384.53, 340.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3537) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 253, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2496) [M::mem_pestat] mean and std.dev: (491.62, 494.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 751854 sequences (100000118 bp) in 3.290 CPU sec, 3.526 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 302966, 17, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 448, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4215) [M::mem_pestat] mean and std.dev: (699.19, 732.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (169.54, 69.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 413, 1712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5024) [M::mem_pestat] mean and std.dev: (793.18, 925.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6680) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 277, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1721) [M::mem_pestat] mean and std.dev: (466.31, 435.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 754116 reads in 178.964 CPU sec, 5.827 real sec [M::process] read 751872 sequences (100000024 bp) in 1.679 CPU sec, 1.691 real sec... sorting block 115, #reads = 1114522 mem = 536883836 peak = 823029760 [M::mem_process_seqs] Processed 752402 reads in 111.458 CPU sec, 3.774 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 302693, 20, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 209, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (292.88, 210.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (170.01, 70.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (106, 429, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1483) [M::mem_pestat] mean and std.dev: (293.94, 193.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1942) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 315, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2628) [M::mem_pestat] mean and std.dev: (512.55, 502.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 116, #reads = 1112188 mem = 536878110 peak = 823029760 [M::process] read 752468 sequences (100000135 bp) in 2.900 CPU sec, 3.037 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 302748, 17, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 294, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2128) [M::mem_pestat] mean and std.dev: (456.90, 497.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (169.84, 70.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (358, 716, 2451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6637) [M::mem_pestat] mean and std.dev: (1242.12, 1588.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8730) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 278, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2129) [M::mem_pestat] mean and std.dev: (505.77, 553.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 751854 reads in 165.449 CPU sec, 5.499 real sec [M::process] read 751556 sequences (100000006 bp) in 2.158 CPU sec, 2.185 real sec... [M::mem_process_seqs] Processed 751872 reads in 108.064 CPU sec, 3.574 real sec sorting block 117, #reads = 1111509 mem = 536887863 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 302980, 26, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 248, 1095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3047) [M::mem_pestat] mean and std.dev: (541.36, 689.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 157, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (169.19, 69.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 562, 1411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3995) [M::mem_pestat] mean and std.dev: (581.21, 623.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 223, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1342) [M::mem_pestat] mean and std.dev: (328.28, 252.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750754 sequences (100000002 bp) in 2.661 CPU sec, 2.798 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 302375, 13, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 377, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1930) [M::mem_pestat] mean and std.dev: (450.08, 352.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (169.84, 70.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (526, 994, 4562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12634) [M::mem_pestat] mean and std.dev: (1689.69, 1825.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 394, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2040) [M::mem_pestat] mean and std.dev: (481.20, 422.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 752468 reads in 156.899 CPU sec, 5.160 real sec [M::process] read 750698 sequences (100000058 bp) in 1.631 CPU sec, 1.651 real sec... [M::mem_process_seqs] Processed 751556 reads in 105.216 CPU sec, 3.539 real sec sorting block 118, #reads = 1112980 mem = 536884875 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 302284, 21, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 265, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195) [M::mem_pestat] mean and std.dev: (282.68, 227.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 158, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (170.79, 70.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (247, 588, 2005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5521) [M::mem_pestat] mean and std.dev: (819.11, 874.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 462, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2690) [M::mem_pestat] mean and std.dev: (701.48, 673.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750412 sequences (100000148 bp) in 2.535 CPU sec, 2.689 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 302459, 13, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 267, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144) [M::mem_pestat] mean and std.dev: (431.24, 414.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (170.67, 70.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 118, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2127) [M::mem_pestat] mean and std.dev: (295.08, 344.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2818) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 429, 1449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4021) [M::mem_pestat] mean and std.dev: (693.09, 682.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 750214 sequences (100000003 bp) in 1.520 CPU sec, 1.521 real sec... [M::mem_process_seqs] Processed 750754 reads in 158.235 CPU sec, 5.252 real sec sorting block 119, #reads = 1114387 mem = 536877116 peak = 823029760 [M::mem_process_seqs] Processed 750698 reads in 101.036 CPU sec, 3.305 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 302157, 16, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 247, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1302) [M::mem_pestat] mean and std.dev: (301.48, 197.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 158, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (170.96, 70.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 390, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4301) [M::mem_pestat] mean and std.dev: (645.50, 873.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 290, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4712) [M::mem_pestat] mean and std.dev: (713.14, 1123.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 120, #reads = 1113220 mem = 536880370 peak = 823029760 [M::process] read 750052 sequences (100000055 bp) in 2.568 CPU sec, 2.696 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 301890, 18, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 402, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2096) [M::mem_pestat] mean and std.dev: (537.70, 418.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (171.09, 70.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 1088, 2319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6371) [M::mem_pestat] mean and std.dev: (1380.72, 1482.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8397) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 271, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (254.23, 121.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 750412 reads in 148.709 CPU sec, 4.823 real sec [M::process] read 750044 sequences (100000271 bp) in 1.613 CPU sec, 1.620 real sec... [M::mem_process_seqs] Processed 750214 reads in 97.479 CPU sec, 3.301 real sec [M::process] read 113572 sequences (15095911 bp) in 0.598 CPU sec, 0.614 real sec... sorting block 121, #reads = 1113606 mem = 536878210 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 302325, 20, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 439, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2747) [M::mem_pestat] mean and std.dev: (729.20, 809.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (117, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (171.28, 71.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 360, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422) [M::mem_pestat] mean and std.dev: (481.89, 479.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3187) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 532, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2598) [M::mem_pestat] mean and std.dev: (598.52, 610.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 45634, 6, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (116, 157, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (169.71, 70.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 750052 reads in 91.792 CPU sec, 3.198 real sec sorting block 122, #reads = 1113960 mem = 536885472 peak = 823029760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 301650, 27, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 270, 2038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5730) [M::mem_pestat] mean and std.dev: (957.63, 1258.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (118, 159, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (171.13, 70.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (113, 560, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3119) [M::mem_pestat] mean and std.dev: (606.84, 672.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4121) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 540, 2090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5890) [M::mem_pestat] mean and std.dev: (1026.52, 1222.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 113572 reads in 93.275 CPU sec, 3.022 real sec [M::mem_process_seqs] Processed 750044 reads in 109.444 CPU sec, 3.557 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 137029874 overall: 69650259968 mem 22822.062 user 865.590 sys 850.457 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_dm4-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:659_dm4-T1-TDNA-1_A23MHWWLT4_1\tLB:659_dm4-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_dm4-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_dm4-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 850.485 sec; CPU: 23687.656 sec sorting block 123, #reads = 1089833 mem = 526405454 peak = 823029760 uniq header count 1 merging 124 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7117513587 output reads: 138222167 bam_mem_sort: 124 calls 97.322 user 2.368 sys 101.113 real bam_write: 124 calls 71.398 user 5.747 sys 121.007 real execute: 1 calls 29.853 user 3.140 sys 889.236 real merge_files: 1 calls 29.826 user 3.116 sys 37.964 real parse_chunk: 1984 calls 91.311 user 25.926 sys 135.072 real read_chunk: 5389 calls 59.438 user 36.123 sys 750.473 real sort_block: 1 calls 47.386 user 34.985 sys 732.939 real write_chunk: 3403 calls 29.181 user 2.416 sys 36.330 real overall: 3386535936 mem 1561.321 user 97.563 sys 889.255 real ls: cannot access '*.cram': No such file or directory