.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_bR4-T1-TDNA-1_A23MHWWLT4_1;L008;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/3a/876b37577b5b7e33e9735a81058627/.command.err
Size: 254.0 KB
Task: DAQ:SENTIEON_BWAMEM (659_bR4-T1-TDNA-1_A23MHWWLT4_1;L008;0001)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_bR4-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1846.600 CPU sec, 78.174 real sec
[M::process] read 676164 sequences (100000283 bp) in 1.665 CPU sec, 1.671 real sec...
[M::process] read 674218 sequences (100000167 bp) in 1.852 CPU sec, 1.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282424, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 341, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (356.74, 158.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673482 sequences (100000117 bp) in 1.898 CPU sec, 2.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281073, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 361, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (374.03, 162.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673682 sequences (100000196 bp) in 1.887 CPU sec, 2.125 real sec...
[M::mem_process_seqs] Processed 676164 reads in 185.266 CPU sec, 6.555 real sec
[M::mem_process_seqs] Processed 674218 reads in 120.108 CPU sec, 4.387 real sec
sorting block 0, #reads = 1080106 mem = 536876870 peak = 566415360
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281676, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 367, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (379.23, 162.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673876 sequences (100000296 bp) in 2.476 CPU sec, 3.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281323, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 365, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (378.73, 163.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675210 sequences (100000247 bp) in 1.992 CPU sec, 2.043 real sec...
[M::mem_process_seqs] Processed 673482 reads in 185.604 CPU sec, 6.861 real sec
[M::mem_process_seqs] Processed 673682 reads in 126.603 CPU sec, 5.273 real sec
sorting block 1, #reads = 1078861 mem = 536878593 peak = 698535936
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280662, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 366, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (379.59, 164.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675630 sequences (100000022 bp) in 2.223 CPU sec, 2.895 real sec...
[M::process] read 675486 sequences (100000142 bp) in 2.035 CPU sec, 2.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281462, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (368.22, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673876 reads in 207.252 CPU sec, 8.465 real sec
[M::mem_process_seqs] Processed 675210 reads in 136.952 CPU sec, 6.021 real sec
sorting block 2, #reads = 1077494 mem = 536880219 peak = 799621120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281889, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (366.84, 163.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675454 sequences (100000043 bp) in 2.882 CPU sec, 3.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281019, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (367.52, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675734 sequences (100000094 bp) in 2.145 CPU sec, 2.243 real sec...
[M::mem_process_seqs] Processed 675630 reads in 216.199 CPU sec, 8.956 real sec
[M::mem_process_seqs] Processed 675486 reads in 140.057 CPU sec, 6.164 real sec
sorting block 3, #reads = 1079213 mem = 536877423 peak = 799621120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281439, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (364.19, 161.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675416 sequences (100000115 bp) in 2.700 CPU sec, 3.206 real sec...
sorting block 4, #reads = 1079474 mem = 536879483 peak = 799621120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281542, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (362.17, 161.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672578 sequences (100000002 bp) in 1.875 CPU sec, 2.011 real sec...
[M::mem_process_seqs] Processed 675454 reads in 221.358 CPU sec, 9.107 real sec
[M::mem_process_seqs] Processed 675734 reads in 141.449 CPU sec, 6.077 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281207, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (365.78, 162.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1079978 mem = 536877470 peak = 799621120
[M::process] read 673570 sequences (100000144 bp) in 2.885 CPU sec, 3.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280094, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 377, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (389.33, 166.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675416 reads in 195.137 CPU sec, 6.910 real sec
[M::mem_process_seqs] Processed 672578 reads in 129.783 CPU sec, 5.073 real sec
[M::process] read 674662 sequences (100000143 bp) in 2.346 CPU sec, 2.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281152, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 367, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (380.72, 165.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 6, #reads = 1079802 mem = 536877418 peak = 799621120
[M::process] read 675446 sequences (100000246 bp) in 2.609 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281387, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 357, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (371.55, 164.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673570 reads in 197.603 CPU sec, 7.215 real sec
[M::mem_process_seqs] Processed 674662 reads in 133.242 CPU sec, 5.294 real sec
[M::process] read 674960 sequences (100000195 bp) in 2.191 CPU sec, 2.449 real sec...
sorting block 7, #reads = 1078654 mem = 536879167 peak = 816373760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281728, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (365.32, 162.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674654 sequences (100000169 bp) in 2.852 CPU sec, 3.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281463, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 354, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (369.64, 163.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675446 reads in 202.895 CPU sec, 7.510 real sec
[M::mem_process_seqs] Processed 674960 reads in 137.104 CPU sec, 5.518 real sec
sorting block 8, #reads = 1080453 mem = 536879082 peak = 816373760
[M::process] read 674734 sequences (100000254 bp) in 2.587 CPU sec, 3.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280992, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (370.04, 163.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674782 sequences (100000059 bp) in 2.802 CPU sec, 3.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280552, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (368.76, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1079721 mem = 536880805 peak = 825262080
[M::mem_process_seqs] Processed 674654 reads in 201.737 CPU sec, 7.602 real sec
[M::mem_process_seqs] Processed 674734 reads in 133.780 CPU sec, 5.566 real sec
[M::process] read 674990 sequences (100000026 bp) in 2.703 CPU sec, 3.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281219, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (367.37, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 10, #reads = 1080889 mem = 536876465 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281471, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (364.28, 160.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673862 sequences (100000142 bp) in 2.956 CPU sec, 3.577 real sec...
[M::mem_process_seqs] Processed 674782 reads in 199.964 CPU sec, 7.251 real sec
[M::mem_process_seqs] Processed 674990 reads in 134.335 CPU sec, 5.309 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281022, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 360, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (374.77, 163.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673216 sequences (100000133 bp) in 2.740 CPU sec, 3.558 real sec...
[M::mem_process_seqs] Processed 673862 reads in 109.690 CPU sec, 4.503 real sec
sorting block 11, #reads = 1079440 mem = 536879150 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280102, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 363, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (377.21, 162.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674482 sequences (100000075 bp) in 2.941 CPU sec, 3.509 real sec...
[M::mem_process_seqs] Processed 673216 reads in 111.666 CPU sec, 4.367 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280809, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (366.39, 160.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 12, #reads = 1079576 mem = 536878925 peak = 825262080
[M::process] read 675042 sequences (100000213 bp) in 2.761 CPU sec, 3.414 real sec...
[M::mem_process_seqs] Processed 674482 reads in 111.451 CPU sec, 4.828 real sec
[M::process] read 674536 sequences (100000081 bp) in 2.694 CPU sec, 3.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280946, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (361.09, 158.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675042 reads in 108.699 CPU sec, 4.266 real sec
sorting block 13, #reads = 1077624 mem = 536881508 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280871, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (367.85, 162.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673142 sequences (100000159 bp) in 2.994 CPU sec, 3.560 real sec...
[M::mem_process_seqs] Processed 674536 reads in 108.784 CPU sec, 4.206 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280769, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 365, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (378.20, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674612 sequences (100000101 bp) in 2.928 CPU sec, 3.728 real sec...
[M::mem_process_seqs] Processed 673142 reads in 107.007 CPU sec, 4.000 real sec
sorting block 14, #reads = 1080202 mem = 536877175 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280714, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (363.79, 159.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674454 sequences (100000294 bp) in 2.809 CPU sec, 3.455 real sec...
[M::mem_process_seqs] Processed 674612 reads in 105.503 CPU sec, 4.127 real sec
sorting block 15, #reads = 1079552 mem = 536879219 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280421, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (364.48, 159.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674276 sequences (100000273 bp) in 2.965 CPU sec, 3.467 real sec...
[M::mem_process_seqs] Processed 674454 reads in 108.126 CPU sec, 3.987 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280911, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (362.44, 157.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674478 sequences (100000167 bp) in 2.670 CPU sec, 3.031 real sec...
[M::mem_process_seqs] Processed 674276 reads in 102.937 CPU sec, 3.680 real sec
sorting block 16, #reads = 1079654 mem = 536879926 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280643, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (364.43, 160.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673350 sequences (100000100 bp) in 2.752 CPU sec, 3.029 real sec...
[M::mem_process_seqs] Processed 674478 reads in 105.582 CPU sec, 3.640 real sec
sorting block 17, #reads = 1080274 mem = 536878206 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280510, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 358, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (372.06, 160.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673134 sequences (100000298 bp) in 2.791 CPU sec, 3.063 real sec...
[M::mem_process_seqs] Processed 673350 reads in 104.214 CPU sec, 3.513 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279883, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 360, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (374.37, 160.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674072 sequences (100000163 bp) in 3.140 CPU sec, 3.383 real sec...
[M::mem_process_seqs] Processed 673134 reads in 101.480 CPU sec, 3.369 real sec
sorting block 18, #reads = 1079167 mem = 536881174 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281056, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 350, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (365.49, 158.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674458 sequences (100000158 bp) in 3.062 CPU sec, 3.290 real sec...
[M::mem_process_seqs] Processed 674072 reads in 98.632 CPU sec, 3.261 real sec
sorting block 19, #reads = 1079159 mem = 536881060 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280519, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (363.88, 159.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674458 reads in 96.737 CPU sec, 3.193 real sec
[M::process] read 674560 sequences (100000259 bp) in 3.224 CPU sec, 3.551 real sec...
sorting block 20, #reads = 1078712 mem = 536878488 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281190, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (362.77, 158.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674192 sequences (100000166 bp) in 2.839 CPU sec, 3.203 real sec...
[M::mem_process_seqs] Processed 674560 reads in 98.160 CPU sec, 3.218 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280212, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (363.90, 158.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673312 sequences (100000031 bp) in 2.806 CPU sec, 2.976 real sec...
[M::mem_process_seqs] Processed 674192 reads in 92.125 CPU sec, 2.990 real sec
sorting block 21, #reads = 1079855 mem = 536878989 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280460, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 358, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (372.56, 160.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674364 sequences (100000157 bp) in 2.640 CPU sec, 2.732 real sec...
[M::mem_process_seqs] Processed 673312 reads in 89.219 CPU sec, 2.876 real sec
sorting block 22, #reads = 1078256 mem = 536877965 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280937, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (367.71, 161.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674170 sequences (100000095 bp) in 2.417 CPU sec, 2.514 real sec...
[M::mem_process_seqs] Processed 674364 reads in 89.905 CPU sec, 2.891 real sec
[M::process] read 674028 sequences (100000023 bp) in 2.552 CPU sec, 2.723 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280495, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (369.70, 163.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673934 sequences (100000059 bp) in 1.794 CPU sec, 1.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281172, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 355, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (370.49, 161.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674170 reads in 159.701 CPU sec, 5.019 real sec
[M::mem_process_seqs] Processed 674028 reads in 105.715 CPU sec, 3.317 real sec
sorting block 23, #reads = 1079479 mem = 536877982 peak = 825262080
[M::process] read 672564 sequences (100000268 bp) in 2.135 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280907, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 359, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (374.35, 164.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1077585 mem = 536877274 peak = 825262080
[M::process] read 675290 sequences (100000255 bp) in 2.444 CPU sec, 2.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279850, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 370, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (384.89, 166.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673934 reads in 155.073 CPU sec, 4.946 real sec
[M::mem_process_seqs] Processed 672564 reads in 102.352 CPU sec, 3.223 real sec
[M::process] read 675382 sequences (100000197 bp) in 1.978 CPU sec, 2.079 real sec...
sorting block 25, #reads = 1078635 mem = 536880454 peak = 825262080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281692, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (363.34, 160.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675014 sequences (100000106 bp) in 1.804 CPU sec, 1.887 real sec...
[M::process] read 674410 sequences (100000192 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281643, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (363.44, 160.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675290 reads in 153.189 CPU sec, 4.881 real sec
[M::mem_process_seqs] Processed 675382 reads in 101.422 CPU sec, 3.225 real sec
sorting block 26, #reads = 1079547 mem = 536878595 peak = 830930944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280948, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 348, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (363.32, 159.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674028 sequences (100000013 bp) in 2.882 CPU sec, 3.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280607, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 359, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (372.99, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675014 reads in 150.472 CPU sec, 4.772 real sec
[M::mem_process_seqs] Processed 674410 reads in 101.210 CPU sec, 3.183 real sec
[M::process] read 675188 sequences (100000171 bp) in 2.334 CPU sec, 2.437 real sec...
sorting block 27, #reads = 1081245 mem = 536880581 peak = 830930944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281097, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 364, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (376.85, 163.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674934 sequences (100000137 bp) in 2.116 CPU sec, 2.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281414, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (369.11, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674028 reads in 152.601 CPU sec, 4.859 real sec
[M::mem_process_seqs] Processed 675188 reads in 102.223 CPU sec, 3.221 real sec
sorting block 28, #reads = 1081090 mem = 536879399 peak = 832454656
[M::process] read 674580 sequences (100000153 bp) in 2.262 CPU sec, 2.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281218, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (371.51, 164.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1081348 mem = 536882329 peak = 850898944
[M::process] read 674622 sequences (100000224 bp) in 2.660 CPU sec, 2.863 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280943, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 359, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (373.66, 165.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674934 reads in 149.958 CPU sec, 4.777 real sec
[M::mem_process_seqs] Processed 674580 reads in 98.287 CPU sec, 3.099 real sec
[M::process] read 674674 sequences (100000145 bp) in 2.099 CPU sec, 2.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281701, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 360, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (373.96, 164.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 30, #reads = 1080921 mem = 536879946 peak = 850898944
[M::process] read 675118 sequences (100000007 bp) in 2.017 CPU sec, 2.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280915, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (369.17, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674622 reads in 146.110 CPU sec, 4.658 real sec
[M::process] read 674662 sequences (100000089 bp) in 1.731 CPU sec, 1.780 real sec...
[M::mem_process_seqs] Processed 674674 reads in 96.767 CPU sec, 3.051 real sec
sorting block 31, #reads = 1081496 mem = 536879364 peak = 850898944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281705, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (368.37, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673546 sequences (100000106 bp) in 2.847 CPU sec, 3.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281178, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 357, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (371.81, 164.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675118 reads in 144.211 CPU sec, 4.587 real sec
[M::mem_process_seqs] Processed 674662 reads in 94.583 CPU sec, 2.992 real sec
[M::process] read 674050 sequences (100000242 bp) in 1.939 CPU sec, 1.999 real sec...
sorting block 32, #reads = 1080996 mem = 536879735 peak = 850898944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280671, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 365, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (378.59, 163.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674900 sequences (100000283 bp) in 2.466 CPU sec, 2.616 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281380, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 361, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (375.14, 164.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673546 reads in 145.826 CPU sec, 4.647 real sec
[M::mem_process_seqs] Processed 674050 reads in 97.216 CPU sec, 3.068 real sec
sorting block 33, #reads = 1081928 mem = 536877532 peak = 892354560
[M::process] read 674768 sequences (100000124 bp) in 2.420 CPU sec, 2.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281823, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 354, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (368.53, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1079823 mem = 536880365 peak = 892354560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281664, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (370.31, 163.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674474 sequences (100000117 bp) in 2.753 CPU sec, 2.983 real sec...
[M::mem_process_seqs] Processed 674900 reads in 149.020 CPU sec, 4.765 real sec
[M::mem_process_seqs] Processed 674768 reads in 98.901 CPU sec, 3.134 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281055, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674758 sequences (100000251 bp) in 2.859 CPU sec, 3.107 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (247, 356, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (371.07, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1081514 mem = 536878054 peak = 892354560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281544, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (369.26, 162.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674474 reads in 149.468 CPU sec, 4.834 real sec
[M::process] read 674754 sequences (100000239 bp) in 2.489 CPU sec, 2.680 real sec...
[M::mem_process_seqs] Processed 674758 reads in 99.279 CPU sec, 3.160 real sec
sorting block 36, #reads = 1081258 mem = 536879032 peak = 892354560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280892, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (369.68, 163.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674252 sequences (100000281 bp) in 2.604 CPU sec, 2.808 real sec...
[M::mem_process_seqs] Processed 674754 reads in 82.159 CPU sec, 2.726 real sec
sorting block 37, #reads = 1080899 mem = 536881616 peak = 892354560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281068, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 357, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (372.75, 164.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674206 sequences (100000208 bp) in 2.568 CPU sec, 2.702 real sec...
[M::mem_process_seqs] Processed 674252 reads in 82.755 CPU sec, 2.705 real sec
[M::process] read 673084 sequences (100000262 bp) in 2.129 CPU sec, 2.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280993, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (370.16, 162.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672616 sequences (100000115 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280778, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 365, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (379.52, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674206 reads in 141.509 CPU sec, 4.448 real sec
[M::mem_process_seqs] Processed 673084 reads in 92.087 CPU sec, 2.895 real sec
sorting block 38, #reads = 1080618 mem = 536879018 peak = 892354560
[M::process] read 674290 sequences (100000040 bp) in 2.058 CPU sec, 2.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280918, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 372, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (385.66, 165.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1080674 mem = 536880431 peak = 892354560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280824, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (368.81, 162.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674510 sequences (100000043 bp) in 2.747 CPU sec, 2.978 real sec...
[M::mem_process_seqs] Processed 672616 reads in 142.654 CPU sec, 4.569 real sec
[M::mem_process_seqs] Processed 674290 reads in 95.022 CPU sec, 3.012 real sec
[M::process] read 674538 sequences (100000297 bp) in 2.092 CPU sec, 2.181 real sec...
sorting block 40, #reads = 1079392 mem = 536878681 peak = 892354560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281125, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (366.67, 161.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281152, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (368.91, 163.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674164 sequences (100000079 bp) in 2.731 CPU sec, 2.889 real sec...
[M::mem_process_seqs] Processed 674510 reads in 146.115 CPU sec, 4.643 real sec
[M::mem_process_seqs] Processed 674538 reads in 96.573 CPU sec, 3.049 real sec
sorting block 41, #reads = 1080628 mem = 536883570 peak = 892354560
[M::process] read 674786 sequences (100000155 bp) in 2.736 CPU sec, 2.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281306, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 357, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (373.31, 165.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280511, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (368.23, 164.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674632 sequences (100000267 bp) in 2.433 CPU sec, 2.596 real sec...
[M::mem_process_seqs] Processed 674164 reads in 145.332 CPU sec, 4.633 real sec
[M::mem_process_seqs] Processed 674786 reads in 95.069 CPU sec, 3.015 real sec
sorting block 42, #reads = 1080745 mem = 536882289 peak = 892354560
[M::process] read 674764 sequences (100000140 bp) in 2.530 CPU sec, 2.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281680, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 354, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (370.48, 165.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281567, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (364.04, 160.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674634 sequences (100000196 bp) in 2.225 CPU sec, 2.331 real sec...
[M::mem_process_seqs] Processed 674632 reads in 142.902 CPU sec, 4.548 real sec
sorting block 43, #reads = 1079561 mem = 536878665 peak = 892354560
[M::mem_process_seqs] Processed 674764 reads in 95.569 CPU sec, 3.022 real sec
[M::process] read 673096 sequences (100000067 bp) in 2.743 CPU sec, 3.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281481, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (362.72, 159.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1080599 mem = 536877043 peak = 892354560
[M::process] read 673478 sequences (100000128 bp) in 2.037 CPU sec, 2.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281058, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 363, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (377.89, 164.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674634 reads in 157.116 CPU sec, 5.025 real sec
[M::mem_process_seqs] Processed 673096 reads in 105.803 CPU sec, 3.344 real sec
[M::process] read 674528 sequences (100000132 bp) in 2.769 CPU sec, 3.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281613, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 359, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (373.80, 162.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1079913 mem = 536878682 peak = 892354560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281121, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (365.47, 162.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674468 sequences (100000136 bp) in 3.001 CPU sec, 3.172 real sec...
[M::mem_process_seqs] Processed 673478 reads in 159.742 CPU sec, 5.107 real sec
[M::mem_process_seqs] Processed 674528 reads in 106.316 CPU sec, 3.362 real sec
sorting block 46, #reads = 1080307 mem = 536878667 peak = 892354560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281560, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (365.74, 161.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674520 sequences (100000075 bp) in 2.939 CPU sec, 3.113 real sec...
[M::mem_process_seqs] Processed 674468 reads in 91.347 CPU sec, 2.991 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281309, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (365.81, 161.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1080603 mem = 536880622 peak = 892354560
[M::mem_process_seqs] Processed 674520 reads in 88.012 CPU sec, 2.886 real sec
[M::process] read 674486 sequences (100000286 bp) in 3.878 CPU sec, 4.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281373, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (365.41, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674390 sequences (100000188 bp) in 2.683 CPU sec, 2.786 real sec...
[M::mem_process_seqs] Processed 674486 reads in 91.738 CPU sec, 2.952 real sec
sorting block 48, #reads = 1080211 mem = 536878319 peak = 893440000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281024, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (366.04, 161.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674390 reads in 89.078 CPU sec, 2.877 real sec
[M::process] read 674552 sequences (100000209 bp) in 3.335 CPU sec, 3.615 real sec...
sorting block 49, #reads = 1079856 mem = 536877777 peak = 893440000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280736, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (366.29, 162.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674588 sequences (100000036 bp) in 2.549 CPU sec, 2.728 real sec...
[M::mem_process_seqs] Processed 674552 reads in 96.236 CPU sec, 3.119 real sec
[M::process] read 674452 sequences (100000166 bp) in 2.415 CPU sec, 2.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281047, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (370.21, 165.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674294 sequences (100000117 bp) in 1.960 CPU sec, 1.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281190, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (370.19, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674588 reads in 162.044 CPU sec, 5.089 real sec
[M::mem_process_seqs] Processed 674452 reads in 106.917 CPU sec, 3.355 real sec
sorting block 50, #reads = 1078757 mem = 536880593 peak = 893440000
[M::process] read 675062 sequences (100000184 bp) in 2.372 CPU sec, 2.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281666, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (369.63, 164.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1079294 mem = 536877644 peak = 935292928
[M::process] read 676054 sequences (100000187 bp) in 2.383 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282105, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (364.95, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674294 reads in 163.103 CPU sec, 5.197 real sec
[M::mem_process_seqs] Processed 675062 reads in 108.686 CPU sec, 3.426 real sec
[M::process] read 675520 sequences (100000015 bp) in 1.996 CPU sec, 2.027 real sec...
sorting block 52, #reads = 1078194 mem = 536880297 peak = 935292928
[M::process] read 675790 sequences (100000083 bp) in 2.968 CPU sec, 3.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281890, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (357.02, 154.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675520 reads in 90.480 CPU sec, 2.839 real sec
[M::process] read 675804 sequences (100000174 bp) in 2.531 CPU sec, 2.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281344, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 343, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (355.75, 155.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282753, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (357.29, 155.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676054 reads in 250.947 CPU sec, 7.938 real sec
sorting block 53, #reads = 1078803 mem = 536877932 peak = 935292928
[M::mem_process_seqs] Processed 675790 reads in 122.208 CPU sec, 3.857 real sec
[M::process] read 675424 sequences (100000293 bp) in 2.795 CPU sec, 2.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281588, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (358.61, 155.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1079488 mem = 536879147 peak = 938561536
[M::process] read 674954 sequences (100000100 bp) in 2.711 CPU sec, 2.845 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281461, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (360.30, 156.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675804 reads in 156.676 CPU sec, 4.988 real sec
[M::mem_process_seqs] Processed 675424 reads in 102.827 CPU sec, 3.237 real sec
[M::process] read 674650 sequences (100000088 bp) in 2.670 CPU sec, 2.771 real sec...
sorting block 55, #reads = 1079267 mem = 536885468 peak = 938561536
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281960, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (360.48, 154.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674462 sequences (100000165 bp) in 2.147 CPU sec, 2.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280954, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (366.11, 158.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674954 reads in 150.100 CPU sec, 4.758 real sec
[M::mem_process_seqs] Processed 674650 reads in 98.613 CPU sec, 3.098 real sec
[M::process] read 673130 sequences (100000118 bp) in 1.983 CPU sec, 2.051 real sec...
sorting block 56, #reads = 1078744 mem = 536878144 peak = 969666560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281241, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 359, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (371.10, 161.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672970 sequences (100000144 bp) in 2.776 CPU sec, 2.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280508, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 374, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (384.59, 163.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674462 reads in 147.086 CPU sec, 4.677 real sec
[M::mem_process_seqs] Processed 673130 reads in 98.046 CPU sec, 3.081 real sec
sorting block 57, #reads = 1078945 mem = 536877035 peak = 969666560
[M::process] read 674802 sequences (100000266 bp) in 2.933 CPU sec, 3.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281449, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 369, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (380.67, 160.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (733, 3093, 3093)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7813)
[M::mem_pestat] mean and std.dev: (1805.60, 1300.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10173)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281584, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (367.08, 160.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675214 sequences (100000038 bp) in 2.549 CPU sec, 2.610 real sec...
[M::mem_process_seqs] Processed 672970 reads in 146.323 CPU sec, 4.648 real sec
sorting block 58, #reads = 1078451 mem = 536879605 peak = 970825728
[M::mem_process_seqs] Processed 674802 reads in 96.661 CPU sec, 3.035 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281451, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (361.63, 158.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 59, #reads = 1079695 mem = 536878878 peak = 978051072
[M::process] read 675248 sequences (100000070 bp) in 3.090 CPU sec, 3.298 real sec...
[M::mem_process_seqs] Processed 675214 reads in 84.294 CPU sec, 2.754 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281349, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (366.82, 161.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675248 reads in 86.219 CPU sec, 2.791 real sec
[M::process] read 675052 sequences (100000048 bp) in 3.169 CPU sec, 3.387 real sec...
sorting block 60, #reads = 1081198 mem = 536879657 peak = 978051072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280635, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (365.50, 160.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675022 sequences (100000156 bp) in 2.333 CPU sec, 2.438 real sec...
[M::mem_process_seqs] Processed 675052 reads in 89.880 CPU sec, 2.881 real sec
sorting block 61, #reads = 1080033 mem = 536878323 peak = 978051072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281288, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (366.33, 159.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674852 sequences (100000179 bp) in 2.900 CPU sec, 3.121 real sec...
[M::mem_process_seqs] Processed 675022 reads in 87.411 CPU sec, 2.816 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281356, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (364.29, 158.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674852 reads in 86.899 CPU sec, 2.790 real sec
[M::process] read 674720 sequences (100000139 bp) in 3.224 CPU sec, 3.475 real sec...
sorting block 62, #reads = 1079753 mem = 536879631 peak = 978051072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281124, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (365.33, 158.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674776 sequences (100000268 bp) in 2.551 CPU sec, 2.711 real sec...
[M::mem_process_seqs] Processed 674720 reads in 89.361 CPU sec, 2.861 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280929, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (367.82, 160.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674772 sequences (100000110 bp) in 2.634 CPU sec, 2.750 real sec...
[M::mem_process_seqs] Processed 674776 reads in 88.672 CPU sec, 2.845 real sec
sorting block 63, #reads = 1079763 mem = 536878930 peak = 978051072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280621, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 356, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (369.87, 162.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674634 sequences (100000162 bp) in 2.774 CPU sec, 2.947 real sec...
[M::mem_process_seqs] Processed 674772 reads in 87.773 CPU sec, 2.820 real sec
sorting block 64, #reads = 1080183 mem = 536878418 peak = 978051072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281577, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (369.95, 161.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674634 reads in 87.133 CPU sec, 2.794 real sec
[M::process] read 673512 sequences (100000130 bp) in 2.769 CPU sec, 2.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281006, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 364, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (376.12, 160.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673010 sequences (100000198 bp) in 2.395 CPU sec, 2.586 real sec...
[M::mem_process_seqs] Processed 673512 reads in 83.602 CPU sec, 2.699 real sec
sorting block 65, #reads = 1080227 mem = 536885302 peak = 978051072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280287, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 368, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (379.36, 160.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673010 reads in 72.329 CPU sec, 2.326 real sec
[M::process] read 673720 sequences (100000051 bp) in 2.307 CPU sec, 2.445 real sec...
sorting block 66, #reads = 1078932 mem = 536878537 peak = 1018699776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280736, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 361, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (374.35, 161.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673720 reads in 77.518 CPU sec, 2.500 real sec
[M::process] read 674502 sequences (100000252 bp) in 2.629 CPU sec, 2.805 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281092, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (364.77, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674354 sequences (100000104 bp) in 2.289 CPU sec, 2.450 real sec...
[M::process] read 674470 sequences (100000252 bp) in 1.370 CPU sec, 1.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281236, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (369.87, 161.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674354 reads in 79.211 CPU sec, 2.477 real sec
sorting block 67, #reads = 1080458 mem = 536877935 peak = 1018699776
[M::process] read 674444 sequences (100000133 bp) in 2.188 CPU sec, 2.261 real sec...
[M::mem_process_seqs] Processed 674502 reads in 207.145 CPU sec, 6.583 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280455, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (368.77, 162.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280737, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (368.39, 162.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674260 sequences (100000119 bp) in 2.769 CPU sec, 2.945 real sec...
[M::mem_process_seqs] Processed 674470 reads in 166.290 CPU sec, 5.251 real sec
sorting block 68, #reads = 1080173 mem = 536879465 peak = 1018699776
[M::mem_process_seqs] Processed 674444 reads in 99.452 CPU sec, 3.141 real sec
sorting block 69, #reads = 1079643 mem = 536878750 peak = 1018699776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280707, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (368.11, 161.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674624 sequences (100000125 bp) in 3.119 CPU sec, 3.398 real sec...
[M::mem_process_seqs] Processed 674260 reads in 86.087 CPU sec, 2.798 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280173, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (367.00, 162.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674624 reads in 85.340 CPU sec, 2.743 real sec
sorting block 70, #reads = 1080125 mem = 536874963 peak = 1018699776
[M::process] read 674738 sequences (100000267 bp) in 3.240 CPU sec, 3.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280375, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (362.05, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674652 sequences (100000251 bp) in 2.600 CPU sec, 2.715 real sec...
[M::mem_process_seqs] Processed 674738 reads in 91.584 CPU sec, 2.945 real sec
sorting block 71, #reads = 1079884 mem = 536879998 peak = 1018699776
[M::process] read 674506 sequences (100000233 bp) in 2.224 CPU sec, 2.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280223, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 346, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (362.12, 159.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674690 sequences (100000231 bp) in 1.420 CPU sec, 1.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280839, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (367.25, 162.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674614 sequences (100000232 bp) in 1.340 CPU sec, 1.333 real sec...
[M::mem_process_seqs] Processed 674652 reads in 158.348 CPU sec, 4.974 real sec
[M::mem_process_seqs] Processed 674506 reads in 103.258 CPU sec, 3.259 real sec
sorting block 72, #reads = 1079775 mem = 536878801 peak = 1018699776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280913, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (362.49, 160.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674592 sequences (100000157 bp) in 2.953 CPU sec, 3.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280171, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (363.47, 160.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674690 reads in 141.163 CPU sec, 4.484 real sec
[M::mem_process_seqs] Processed 674614 reads in 86.916 CPU sec, 2.738 real sec
sorting block 73, #reads = 1080911 mem = 536881933 peak = 1018699776
[M::process] read 674426 sequences (100000133 bp) in 2.747 CPU sec, 2.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281126, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (364.39, 160.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674396 sequences (100000212 bp) in 1.614 CPU sec, 1.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281197, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (365.12, 160.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674592 reads in 132.076 CPU sec, 4.191 real sec
[M::mem_process_seqs] Processed 674426 reads in 82.356 CPU sec, 2.588 real sec
sorting block 74, #reads = 1079934 mem = 536879773 peak = 1018699776
[M::process] read 674676 sequences (100000203 bp) in 2.072 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280957, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (367.07, 161.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674270 sequences (100000035 bp) in 2.120 CPU sec, 2.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281528, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (366.22, 162.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674396 reads in 124.521 CPU sec, 3.956 real sec
sorting block 75, #reads = 1080533 mem = 536881028 peak = 1018699776
[M::mem_process_seqs] Processed 674676 reads in 80.137 CPU sec, 2.517 real sec
[M::process] read 674398 sequences (100000219 bp) in 1.995 CPU sec, 2.077 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280967, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (367.70, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 76, #reads = 1080810 mem = 536878055 peak = 1109950464
[M::process] read 674572 sequences (100000131 bp) in 2.043 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280835, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (368.40, 163.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674270 reads in 125.704 CPU sec, 3.982 real sec
[M::mem_process_seqs] Processed 674398 reads in 81.198 CPU sec, 2.553 real sec
[M::process] read 674902 sequences (100000072 bp) in 1.983 CPU sec, 2.079 real sec...
sorting block 77, #reads = 1079135 mem = 536876980 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281160, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (369.25, 166.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674924 sequences (100000041 bp) in 2.291 CPU sec, 2.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281029, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (364.01, 160.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674572 reads in 135.774 CPU sec, 4.305 real sec
[M::mem_process_seqs] Processed 674902 reads in 89.796 CPU sec, 2.820 real sec
[M::process] read 674324 sequences (100000139 bp) in 2.151 CPU sec, 2.217 real sec...
sorting block 78, #reads = 1079452 mem = 536876878 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280876, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 349, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (362.17, 156.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674050 sequences (100000037 bp) in 2.090 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281006, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 348, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (361.46, 154.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674924 reads in 133.365 CPU sec, 4.239 real sec
[M::mem_process_seqs] Processed 674324 reads in 86.170 CPU sec, 2.709 real sec
sorting block 79, #reads = 1078652 mem = 536878743 peak = 1109950464
[M::process] read 674110 sequences (100000146 bp) in 2.176 CPU sec, 2.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280457, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 357, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (369.10, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673858 sequences (100000120 bp) in 2.279 CPU sec, 2.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279762, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 354, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (365.94, 156.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674050 reads in 134.630 CPU sec, 4.272 real sec
sorting block 80, #reads = 1079701 mem = 536877415 peak = 1109950464
[M::mem_process_seqs] Processed 674110 reads in 86.182 CPU sec, 2.707 real sec
[M::process] read 674662 sequences (100000182 bp) in 2.269 CPU sec, 2.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280653, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 356, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (368.57, 157.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 81, #reads = 1079301 mem = 536876979 peak = 1109950464
[M::process] read 674594 sequences (100000248 bp) in 2.242 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280177, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 349, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (361.95, 156.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673858 reads in 135.744 CPU sec, 4.318 real sec
[M::mem_process_seqs] Processed 674662 reads in 87.321 CPU sec, 2.751 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280652, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 349, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (361.93, 155.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674844 sequences (100000156 bp) in 2.950 CPU sec, 3.196 real sec...
sorting block 82, #reads = 1079720 mem = 536876084 peak = 1109950464
[M::mem_process_seqs] Processed 674594 reads in 74.067 CPU sec, 2.438 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281251, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 347, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (360.96, 156.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674844 reads in 75.200 CPU sec, 2.465 real sec
[M::process] read 674014 sequences (100000146 bp) in 3.039 CPU sec, 3.401 real sec...
sorting block 83, #reads = 1080525 mem = 536878004 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280678, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 360, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (372.12, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674014 reads in 74.928 CPU sec, 2.440 real sec
[M::process] read 672810 sequences (100000234 bp) in 2.778 CPU sec, 3.023 real sec...
sorting block 84, #reads = 1080246 mem = 536880605 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279663, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 369, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (380.28, 161.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672810 reads in 73.665 CPU sec, 2.371 real sec
[M::process] read 672810 sequences (100000120 bp) in 2.377 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279519, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 369, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (380.98, 161.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672810 reads in 74.716 CPU sec, 2.411 real sec
sorting block 85, #reads = 1079590 mem = 536877873 peak = 1109950464
[M::process] read 675192 sequences (100000245 bp) in 2.903 CPU sec, 3.178 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280927, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (365.19, 160.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674872 sequences (100000090 bp) in 1.962 CPU sec, 2.024 real sec...
[M::mem_process_seqs] Processed 675192 reads in 75.507 CPU sec, 2.418 real sec
sorting block 86, #reads = 1080526 mem = 536880212 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280477, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (366.28, 159.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674872 reads in 77.342 CPU sec, 2.490 real sec
[M::process] read 675000 sequences (100000239 bp) in 2.724 CPU sec, 2.963 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281268, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (366.66, 160.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675408 sequences (100000026 bp) in 2.336 CPU sec, 2.475 real sec...
[M::mem_process_seqs] Processed 675000 reads in 77.289 CPU sec, 2.489 real sec
sorting block 87, #reads = 1080308 mem = 536879278 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281464, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (362.43, 158.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675284 sequences (100000122 bp) in 2.266 CPU sec, 2.425 real sec...
[M::mem_process_seqs] Processed 675408 reads in 77.582 CPU sec, 2.492 real sec
sorting block 88, #reads = 1080614 mem = 536881034 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281577, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (361.30, 157.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675344 sequences (100000050 bp) in 2.217 CPU sec, 2.317 real sec...
[M::mem_process_seqs] Processed 675284 reads in 77.293 CPU sec, 2.481 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280572, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (361.13, 158.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675242 sequences (100000204 bp) in 2.327 CPU sec, 2.421 real sec...
[M::mem_process_seqs] Processed 675344 reads in 77.569 CPU sec, 2.482 real sec
sorting block 89, #reads = 1080593 mem = 536879873 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280665, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (364.20, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675088 sequences (100000145 bp) in 2.303 CPU sec, 2.465 real sec...
[M::mem_process_seqs] Processed 675242 reads in 77.649 CPU sec, 2.490 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281042, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (363.83, 159.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674800 sequences (100000177 bp) in 2.555 CPU sec, 2.653 real sec...
[M::mem_process_seqs] Processed 675088 reads in 77.595 CPU sec, 2.500 real sec
sorting block 90, #reads = 1079434 mem = 536879585 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280777, 3, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (317, 317, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (317, 317)
[M::mem_pestat] mean and std.dev: (317.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (317, 317)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (365.88, 159.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674770 sequences (100000268 bp) in 2.158 CPU sec, 2.267 real sec...
[M::mem_process_seqs] Processed 674800 reads in 75.097 CPU sec, 2.411 real sec
sorting block 91, #reads = 1079841 mem = 536878360 peak = 1109950464
[M::process] read 673340 sequences (100000148 bp) in 2.156 CPU sec, 2.262 real sec...
[M::process] read 673380 sequences (100000063 bp) in 1.500 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280408, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 367, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (379.16, 161.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673340 reads in 74.561 CPU sec, 2.330 real sec
[M::process] read 674574 sequences (100000054 bp) in 1.942 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281227, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (367.01, 160.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280753, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 363, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (376.46, 160.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674770 reads in 212.307 CPU sec, 6.663 real sec
[M::mem_process_seqs] Processed 673380 reads in 101.295 CPU sec, 3.190 real sec
sorting block 92, #reads = 1080503 mem = 536876023 peak = 1109950464
[M::process] read 674672 sequences (100000226 bp) in 2.228 CPU sec, 2.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280760, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (369.65, 161.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (381, 2001, 2632)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7134)
[M::mem_pestat] mean and std.dev: (1599.18, 1039.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9385)
[M::mem_pestat] skip orientation RR
sorting block 93, #reads = 1080275 mem = 536879504 peak = 1109950464
[M::process] read 674622 sequences (100000049 bp) in 2.120 CPU sec, 2.269 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280098, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (366.34, 160.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674574 reads in 142.409 CPU sec, 4.521 real sec
[M::process] read 674068 sequences (100000203 bp) in 1.609 CPU sec, 1.613 real sec...
[M::mem_process_seqs] Processed 674672 reads in 93.416 CPU sec, 2.932 real sec
sorting block 94, #reads = 1079855 mem = 536876588 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280694, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (367.42, 160.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673462 sequences (100000092 bp) in 2.448 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280649, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 356, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (369.73, 160.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674256 sequences (100000153 bp) in 1.490 CPU sec, 1.486 real sec...
[M::mem_process_seqs] Processed 674622 reads in 142.534 CPU sec, 4.510 real sec
[M::mem_process_seqs] Processed 674068 reads in 91.699 CPU sec, 2.884 real sec
sorting block 95, #reads = 1080122 mem = 536881649 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279659, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 364, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (377.03, 161.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674470 sequences (100000195 bp) in 2.138 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280767, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (369.10, 160.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674408 sequences (100000118 bp) in 1.590 CPU sec, 1.591 real sec...
[M::mem_process_seqs] Processed 673462 reads in 141.424 CPU sec, 4.474 real sec
[M::mem_process_seqs] Processed 674256 reads in 89.196 CPU sec, 2.817 real sec
sorting block 96, #reads = 1079776 mem = 536878668 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280479, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (366.03, 159.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674720 sequences (100000072 bp) in 2.530 CPU sec, 2.670 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280509, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (367.53, 160.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674726 sequences (100000058 bp) in 1.590 CPU sec, 1.585 real sec...
[M::mem_process_seqs] Processed 674470 reads in 143.523 CPU sec, 4.538 real sec
sorting block 97, #reads = 1079326 mem = 536877475 peak = 1109950464
[M::mem_process_seqs] Processed 674408 reads in 91.139 CPU sec, 2.874 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280380, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (361.40, 157.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 98, #reads = 1079721 mem = 536877886 peak = 1109950464
[M::process] read 674686 sequences (100000270 bp) in 2.275 CPU sec, 2.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280819, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (362.88, 158.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674774 sequences (100000004 bp) in 1.610 CPU sec, 1.615 real sec...
[M::mem_process_seqs] Processed 674720 reads in 143.937 CPU sec, 4.556 real sec
[M::mem_process_seqs] Processed 674726 reads in 90.691 CPU sec, 2.857 real sec
sorting block 99, #reads = 1079321 mem = 536877655 peak = 1109950464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280782, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (362.21, 158.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674858 sequences (100000213 bp) in 2.343 CPU sec, 2.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281104, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (361.64, 158.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674650 sequences (100000141 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 674686 reads in 141.795 CPU sec, 4.491 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281407, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (361.99, 159.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674510 sequences (100000071 bp) in 1.717 CPU sec, 1.719 real sec...
[M::mem_process_seqs] Processed 674858 reads in 76.374 CPU sec, 2.413 real sec
sorting block 100, #reads = 1078631 mem = 536877228 peak = 1109950464
[M::mem_process_seqs] Processed 674774 reads in 184.145 CPU sec, 5.831 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281098, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (363.60, 159.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 101, #reads = 1079377 mem = 536879900 peak = 1141477376
[M::process] read 674526 sequences (100000062 bp) in 2.613 CPU sec, 2.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281001, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (366.24, 161.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674650 reads in 152.984 CPU sec, 4.841 real sec
[M::mem_process_seqs] Processed 674510 reads in 90.708 CPU sec, 2.845 real sec
[M::process] read 674456 sequences (100000158 bp) in 2.180 CPU sec, 2.252 real sec...
sorting block 102, #reads = 1079454 mem = 536879500 peak = 1141477376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280843, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (366.30, 160.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674794 sequences (100000021 bp) in 2.174 CPU sec, 2.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280897, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (366.75, 161.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674526 reads in 140.617 CPU sec, 4.461 real sec
[M::process] read 676510 sequences (100000041 bp) in 2.118 CPU sec, 2.182 real sec...
sorting block 103, #reads = 1079167 mem = 536881191 peak = 1141477376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281123, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (364.46, 162.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674794 reads in 77.004 CPU sec, 2.425 real sec
[M::process] read 678240 sequences (100000171 bp) in 2.449 CPU sec, 2.513 real sec...
[M::mem_process_seqs] Processed 674456 reads in 205.465 CPU sec, 6.468 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282222, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (356.20, 162.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 104, #reads = 1078660 mem = 536878461 peak = 1141477376
[M::process] read 675736 sequences (100000215 bp) in 2.497 CPU sec, 2.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282030, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (353.82, 166.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676510 reads in 165.479 CPU sec, 5.215 real sec
[M::mem_process_seqs] Processed 678240 reads in 100.866 CPU sec, 3.170 real sec
sorting block 105, #reads = 1077649 mem = 536880227 peak = 1141477376
[M::process] read 675940 sequences (100000206 bp) in 2.309 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281728, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 357, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (372.94, 168.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676266 sequences (100000013 bp) in 2.290 CPU sec, 2.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281669, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 355, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (371.89, 169.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677342 sequences (100000233 bp) in 1.400 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 675736 reads in 148.762 CPU sec, 4.708 real sec
sorting block 106, #reads = 1075809 mem = 536877693 peak = 1141477376
[M::mem_process_seqs] Processed 675940 reads in 94.851 CPU sec, 2.985 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282468, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 352, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (368.76, 167.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677388 sequences (100000166 bp) in 2.448 CPU sec, 2.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282189, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (355.54, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677028 sequences (100000008 bp) in 1.460 CPU sec, 1.458 real sec...
[M::mem_process_seqs] Processed 676266 reads in 143.635 CPU sec, 4.546 real sec
sorting block 107, #reads = 1076065 mem = 536879382 peak = 1148858368
[M::mem_process_seqs] Processed 677342 reads in 92.623 CPU sec, 2.928 real sec
sorting block 108, #reads = 1076776 mem = 536887753 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281474, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (356.26, 162.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 256, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1135)
[M::mem_pestat] mean and std.dev: (354.75, 140.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1435)
[M::mem_pestat] skip orientation RR
[M::process] read 677236 sequences (100000261 bp) in 2.198 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282044, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 341, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (358.71, 164.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677388 reads in 143.340 CPU sec, 4.536 real sec
[M::process] read 677160 sequences (100000238 bp) in 1.839 CPU sec, 1.843 real sec...
[M::mem_process_seqs] Processed 677028 reads in 91.344 CPU sec, 2.871 real sec
sorting block 109, #reads = 1077388 mem = 536880717 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282059, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (355.11, 162.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674188 sequences (100000054 bp) in 2.619 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281880, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (354.66, 161.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677236 reads in 140.487 CPU sec, 4.450 real sec
[M::mem_process_seqs] Processed 677160 reads in 90.647 CPU sec, 2.848 real sec
[M::process] read 675588 sequences (100000007 bp) in 2.161 CPU sec, 2.226 real sec...
sorting block 110, #reads = 1077923 mem = 536879052 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281286, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 367, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (380.86, 167.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677162 sequences (100000224 bp) in 2.267 CPU sec, 2.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281919, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 353, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (366.60, 162.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674188 reads in 138.548 CPU sec, 4.407 real sec
[M::mem_process_seqs] Processed 675588 reads in 89.113 CPU sec, 2.799 real sec
sorting block 111, #reads = 1076313 mem = 536879635 peak = 1148858368
[M::process] read 677256 sequences (100000292 bp) in 2.283 CPU sec, 2.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282388, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (354.76, 160.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677394 sequences (100000017 bp) in 2.220 CPU sec, 2.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281997, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (355.23, 161.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 260, 933)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2279)
[M::mem_pestat] mean and std.dev: (625.40, 639.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3185)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677162 reads in 144.645 CPU sec, 4.587 real sec
sorting block 112, #reads = 1075659 mem = 536877894 peak = 1148858368
[M::mem_process_seqs] Processed 677256 reads in 94.223 CPU sec, 2.962 real sec
[M::process] read 676736 sequences (100000003 bp) in 1.955 CPU sec, 1.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282300, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 113, #reads = 1077269 mem = 536879860 peak = 1148858368
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (350.90, 158.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676120 sequences (100000176 bp) in 2.330 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282439, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 345, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (360.93, 162.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677394 reads in 147.411 CPU sec, 4.678 real sec
[M::process] read 675764 sequences (100000239 bp) in 1.884 CPU sec, 1.912 real sec...
[M::mem_process_seqs] Processed 676736 reads in 95.540 CPU sec, 3.007 real sec
sorting block 114, #reads = 1075752 mem = 536881982 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282298, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 348, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (363.65, 162.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673870 sequences (100000113 bp) in 2.434 CPU sec, 2.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281729, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (363.64, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676120 reads in 145.435 CPU sec, 4.604 real sec
[M::process] read 673994 sequences (100000021 bp) in 1.727 CPU sec, 1.724 real sec...
[M::mem_process_seqs] Processed 675764 reads in 93.246 CPU sec, 2.940 real sec
sorting block 115, #reads = 1076838 mem = 536876224 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281662, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 367, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (380.11, 164.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675666 sequences (100000199 bp) in 2.260 CPU sec, 2.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281506, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 365, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (377.69, 164.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673870 reads in 143.251 CPU sec, 4.542 real sec
[M::process] read 675918 sequences (100000005 bp) in 1.848 CPU sec, 1.859 real sec...
[M::mem_process_seqs] Processed 673994 reads in 92.866 CPU sec, 2.919 real sec
sorting block 116, #reads = 1076805 mem = 536878584 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281403, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 351, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (365.84, 162.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674040 sequences (100000193 bp) in 2.609 CPU sec, 2.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282023, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 349, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (363.19, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675666 reads in 147.478 CPU sec, 4.666 real sec
sorting block 117, #reads = 1075280 mem = 536876565 peak = 1148858368
[M::process] read 674938 sequences (100000065 bp) in 1.859 CPU sec, 1.871 real sec...
[M::mem_process_seqs] Processed 675918 reads in 93.740 CPU sec, 2.951 real sec
sorting block 118, #reads = 1076362 mem = 536881031 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280847, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 362, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (375.30, 163.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674998 sequences (100000095 bp) in 2.614 CPU sec, 2.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281363, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 353, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (365.45, 159.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674040 reads in 145.715 CPU sec, 4.617 real sec
[M::process] read 674718 sequences (100000272 bp) in 1.947 CPU sec, 1.976 real sec...
[M::mem_process_seqs] Processed 674938 reads in 95.226 CPU sec, 2.989 real sec
sorting block 119, #reads = 1074713 mem = 536882123 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281749, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (361.69, 157.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675062 sequences (100000291 bp) in 2.530 CPU sec, 2.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281816, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (364.57, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674998 reads in 145.554 CPU sec, 4.618 real sec
[M::process] read 673998 sequences (100000051 bp) in 1.927 CPU sec, 1.950 real sec...
[M::mem_process_seqs] Processed 674718 reads in 92.602 CPU sec, 2.914 real sec
sorting block 120, #reads = 1075446 mem = 536883817 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281129, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (364.14, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673708 sequences (100000016 bp) in 2.481 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280981, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 364, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (376.60, 163.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675062 reads in 142.048 CPU sec, 4.507 real sec
[M::mem_process_seqs] Processed 673998 reads in 91.599 CPU sec, 2.875 real sec
sorting block 121, #reads = 1076412 mem = 536878746 peak = 1148858368
[M::process] read 675184 sequences (100000146 bp) in 2.046 CPU sec, 2.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280829, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 365, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (377.13, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 122, #reads = 1076370 mem = 536879171 peak = 1148858368
[M::process] read 675096 sequences (100000051 bp) in 2.401 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281735, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (362.26, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673708 reads in 142.575 CPU sec, 4.517 real sec
[M::mem_process_seqs] Processed 675184 reads in 91.859 CPU sec, 2.886 real sec
[M::process] read 674776 sequences (100000093 bp) in 1.981 CPU sec, 2.012 real sec...
sorting block 123, #reads = 1074835 mem = 536879227 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281949, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (361.07, 157.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673646 sequences (100000186 bp) in 2.617 CPU sec, 2.739 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281988, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (363.04, 158.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675096 reads in 140.445 CPU sec, 4.456 real sec
[M::mem_process_seqs] Processed 674776 reads in 89.829 CPU sec, 2.826 real sec
[M::process] read 674772 sequences (100000235 bp) in 2.024 CPU sec, 2.093 real sec...
sorting block 124, #reads = 1076130 mem = 536880636 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281278, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 363, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (375.77, 162.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674758 sequences (100000060 bp) in 2.364 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281243, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (367.30, 162.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673646 reads in 140.309 CPU sec, 4.447 real sec
[M::mem_process_seqs] Processed 674772 reads in 91.035 CPU sec, 2.856 real sec
[M::process] read 674846 sequences (100000134 bp) in 1.997 CPU sec, 2.037 real sec...
sorting block 125, #reads = 1075277 mem = 536879445 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281902, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (367.11, 161.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674664 sequences (100000170 bp) in 2.585 CPU sec, 2.693 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281285, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (368.45, 163.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674758 reads in 140.690 CPU sec, 4.457 real sec
[M::mem_process_seqs] Processed 674846 reads in 90.093 CPU sec, 2.830 real sec
sorting block 126, #reads = 1075548 mem = 536881394 peak = 1148858368
[M::process] read 673298 sequences (100000246 bp) in 1.990 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281459, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (369.94, 164.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 127, #reads = 1075548 mem = 536880696 peak = 1148858368
[M::process] read 680392 sequences (100000282 bp) in 2.517 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280909, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 370, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (383.66, 168.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674664 reads in 140.134 CPU sec, 4.437 real sec
[M::mem_process_seqs] Processed 673298 reads in 89.057 CPU sec, 2.793 real sec
[M::process] read 679806 sequences (100000117 bp) in 2.037 CPU sec, 2.075 real sec...
sorting block 128, #reads = 1074962 mem = 536881226 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283362, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (332.49, 152.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678178 sequences (100000177 bp) in 2.370 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283515, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 319, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (335.55, 152.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678486 sequences (100000299 bp) in 1.670 CPU sec, 1.668 real sec...
[M::mem_process_seqs] Processed 680392 reads in 154.909 CPU sec, 4.905 real sec
[M::mem_process_seqs] Processed 679806 reads in 99.536 CPU sec, 3.142 real sec
sorting block 129, #reads = 1070821 mem = 536879324 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282981, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (346.14, 154.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678288 sequences (100000102 bp) in 2.696 CPU sec, 2.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283122, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (341.31, 151.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677778 sequences (100000132 bp) in 1.809 CPU sec, 1.811 real sec...
[M::mem_process_seqs] Processed 678178 reads in 155.577 CPU sec, 4.917 real sec
sorting block 130, #reads = 1070396 mem = 536879029 peak = 1148858368
[M::mem_process_seqs] Processed 678486 reads in 100.040 CPU sec, 3.158 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283801, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (344.12, 153.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677780 sequences (100000174 bp) in 2.568 CPU sec, 2.661 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282512, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (348.02, 155.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678258 sequences (100000077 bp) in 1.830 CPU sec, 1.825 real sec...
[M::mem_process_seqs] Processed 678288 reads in 147.808 CPU sec, 4.667 real sec
sorting block 131, #reads = 1069869 mem = 536878051 peak = 1148858368
[M::mem_process_seqs] Processed 677778 reads in 93.498 CPU sec, 2.938 real sec
sorting block 132, #reads = 1073138 mem = 536876636 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282772, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (346.29, 154.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676864 sequences (100000199 bp) in 2.675 CPU sec, 2.792 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283043, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (343.37, 153.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677780 reads in 147.707 CPU sec, 4.669 real sec
[M::process] read 676308 sequences (100000060 bp) in 1.889 CPU sec, 1.890 real sec...
[M::mem_process_seqs] Processed 678258 reads in 95.228 CPU sec, 2.997 real sec
sorting block 133, #reads = 1073345 mem = 536877431 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282837, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (353.23, 157.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677528 sequences (100000095 bp) in 2.286 CPU sec, 2.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282373, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (357.77, 159.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676864 reads in 138.369 CPU sec, 4.385 real sec
[M::process] read 677698 sequences (100000017 bp) in 1.798 CPU sec, 1.814 real sec...
[M::mem_process_seqs] Processed 676308 reads in 87.735 CPU sec, 2.756 real sec
sorting block 134, #reads = 1073480 mem = 536879373 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282668, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (347.46, 155.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677606 sequences (100000176 bp) in 2.414 CPU sec, 2.608 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282531, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (347.38, 155.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677528 reads in 137.073 CPU sec, 4.353 real sec
[M::process] read 677626 sequences (100000089 bp) in 1.593 CPU sec, 1.608 real sec...
[M::mem_process_seqs] Processed 677698 reads in 88.926 CPU sec, 2.805 real sec
sorting block 135, #reads = 1075367 mem = 536878204 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282629, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (347.96, 156.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676806 sequences (100000225 bp) in 2.437 CPU sec, 2.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282981, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (348.95, 156.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677606 reads in 137.530 CPU sec, 4.374 real sec
sorting block 136, #reads = 1075020 mem = 536879507 peak = 1148858368
[M::mem_process_seqs] Processed 677626 reads in 88.278 CPU sec, 2.786 real sec
[M::process] read 676358 sequences (100000143 bp) in 1.710 CPU sec, 1.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283038, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (354.47, 159.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 137, #reads = 1075063 mem = 536880502 peak = 1148858368
[M::process] read 674506 sequences (100000101 bp) in 2.437 CPU sec, 2.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282593, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 345, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (359.77, 160.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676806 reads in 139.848 CPU sec, 4.445 real sec
[M::mem_process_seqs] Processed 676358 reads in 90.030 CPU sec, 2.838 real sec
[M::process] read 675086 sequences (100000056 bp) in 1.739 CPU sec, 1.786 real sec...
sorting block 138, #reads = 1074202 mem = 536891393 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281508, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 361, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (373.99, 163.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676008 sequences (100000193 bp) in 2.372 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282174, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 359, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (371.80, 164.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674506 reads in 136.301 CPU sec, 4.347 real sec
[M::mem_process_seqs] Processed 675086 reads in 89.559 CPU sec, 2.831 real sec
[M::process] read 675554 sequences (100000160 bp) in 1.752 CPU sec, 1.806 real sec...
sorting block 139, #reads = 1075010 mem = 536879143 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282359, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (361.37, 161.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674712 sequences (100000128 bp) in 2.360 CPU sec, 2.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282270, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 352, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (367.67, 165.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676008 reads in 138.474 CPU sec, 4.405 real sec
[M::mem_process_seqs] Processed 675554 reads in 89.967 CPU sec, 2.842 real sec
sorting block 140, #reads = 1074277 mem = 536877449 peak = 1148858368
[M::process] read 675130 sequences (100000032 bp) in 1.914 CPU sec, 1.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281168, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 356, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (371.88, 166.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 141, #reads = 1075346 mem = 536879969 peak = 1148858368
[M::process] read 674960 sequences (100000269 bp) in 2.420 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282012, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (366.73, 162.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674712 reads in 138.791 CPU sec, 4.415 real sec
[M::mem_process_seqs] Processed 675130 reads in 90.922 CPU sec, 2.869 real sec
[M::process] read 675068 sequences (100000099 bp) in 2.129 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281471, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (368.80, 163.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 142, #reads = 1075142 mem = 536877110 peak = 1148858368
[M::process] read 674336 sequences (100000109 bp) in 2.246 CPU sec, 2.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281720, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (367.48, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674960 reads in 136.727 CPU sec, 4.347 real sec
[M::mem_process_seqs] Processed 675068 reads in 88.967 CPU sec, 2.808 real sec
[M::process] read 673398 sequences (100000168 bp) in 1.780 CPU sec, 1.837 real sec...
sorting block 143, #reads = 1075593 mem = 536878680 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281531, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 359, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (374.23, 165.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674450 sequences (100000106 bp) in 2.765 CPU sec, 2.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281755, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 370, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (383.11, 167.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674336 reads in 135.638 CPU sec, 4.323 real sec
[M::mem_process_seqs] Processed 673398 reads in 87.146 CPU sec, 2.758 real sec
sorting block 144, #reads = 1075155 mem = 536878346 peak = 1148858368
[M::process] read 675144 sequences (100000123 bp) in 2.300 CPU sec, 2.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281902, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 359, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (374.35, 165.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675186 sequences (100000096 bp) in 2.265 CPU sec, 2.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281793, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (367.65, 164.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674450 reads in 136.011 CPU sec, 4.335 real sec
[M::mem_process_seqs] Processed 675144 reads in 88.304 CPU sec, 2.791 real sec
sorting block 145, #reads = 1075163 mem = 536879720 peak = 1148858368
[M::process] read 674906 sequences (100000250 bp) in 1.928 CPU sec, 2.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282199, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (365.63, 163.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 146, #reads = 1076380 mem = 536877185 peak = 1148858368
[M::process] read 674650 sequences (100000207 bp) in 2.209 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281692, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (368.99, 164.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675186 reads in 135.608 CPU sec, 4.316 real sec
[M::mem_process_seqs] Processed 674906 reads in 88.358 CPU sec, 2.786 real sec
[M::process] read 674714 sequences (100000043 bp) in 1.821 CPU sec, 1.884 real sec...
sorting block 147, #reads = 1076447 mem = 536880647 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280721, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 357, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (374.14, 169.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675268 sequences (100000119 bp) in 2.228 CPU sec, 2.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281066, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 356, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (374.05, 169.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674650 reads in 135.647 CPU sec, 4.308 real sec
[M::process] read 674942 sequences (100000166 bp) in 1.670 CPU sec, 1.700 real sec...
[M::mem_process_seqs] Processed 674714 reads in 89.021 CPU sec, 2.812 real sec
sorting block 148, #reads = 1075256 mem = 536877716 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282119, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 354, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (371.47, 169.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675472 sequences (100000204 bp) in 2.530 CPU sec, 2.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281367, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 353, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (370.59, 167.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675268 reads in 137.960 CPU sec, 4.381 real sec
[M::mem_process_seqs] Processed 674942 reads in 91.582 CPU sec, 2.884 real sec
[M::process] read 678386 sequences (100000152 bp) in 1.837 CPU sec, 1.874 real sec...
sorting block 149, #reads = 1074020 mem = 536880092 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281824, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 351, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (368.46, 167.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677412 sequences (100000209 bp) in 2.354 CPU sec, 2.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282601, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (344.12, 155.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677524 sequences (100000289 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 675472 reads in 152.787 CPU sec, 4.858 real sec
sorting block 150, #reads = 1072672 mem = 536878360 peak = 1148858368
[M::mem_process_seqs] Processed 678386 reads in 97.658 CPU sec, 3.094 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282442, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (351.21, 157.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 151, #reads = 1066541 mem = 536878695 peak = 1148858368
[M::process] read 677184 sequences (100000123 bp) in 2.248 CPU sec, 2.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283078, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (353.66, 158.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677412 reads in 132.595 CPU sec, 4.204 real sec
[M::process] read 676656 sequences (100000066 bp) in 1.636 CPU sec, 1.641 real sec...
[M::mem_process_seqs] Processed 677524 reads in 85.342 CPU sec, 2.698 real sec
sorting block 152, #reads = 1077325 mem = 536876746 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282430, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 339, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (353.49, 158.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676680 sequences (100000042 bp) in 2.376 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281731, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 341, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (354.69, 157.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677184 reads in 129.816 CPU sec, 4.125 real sec
[M::mem_process_seqs] Processed 676656 reads in 84.250 CPU sec, 2.658 real sec
[M::process] read 676774 sequences (100000146 bp) in 1.891 CPU sec, 1.941 real sec...
sorting block 153, #reads = 1077776 mem = 536880413 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281943, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 342, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (356.24, 158.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676228 sequences (100000150 bp) in 2.350 CPU sec, 2.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281867, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 343, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (357.12, 159.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676680 reads in 129.226 CPU sec, 4.115 real sec
[M::mem_process_seqs] Processed 676774 reads in 84.129 CPU sec, 2.659 real sec
[M::process] read 674268 sequences (100000300 bp) in 2.058 CPU sec, 2.185 real sec...
sorting block 154, #reads = 1078585 mem = 536877871 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281774, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 348, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (361.77, 161.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281048, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 360, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (370.58, 158.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (578, 1777, 2429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6131)
[M::mem_pestat] mean and std.dev: (1578.15, 901.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7982)
[M::mem_pestat] skip orientation RR
[M::process] read 674992 sequences (100000125 bp) in 2.610 CPU sec, 2.866 real sec...
[M::mem_process_seqs] Processed 676228 reads in 126.876 CPU sec, 4.071 real sec
sorting block 155, #reads = 1078188 mem = 536878256 peak = 1148858368
[M::mem_process_seqs] Processed 674268 reads in 82.298 CPU sec, 2.619 real sec
[M::process] read 677432 sequences (100000161 bp) in 2.047 CPU sec, 2.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281360, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 356, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (367.34, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 156, #reads = 1077307 mem = 536880316 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282090, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (349.27, 156.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676960 sequences (100000268 bp) in 2.112 CPU sec, 2.289 real sec...
[M::mem_process_seqs] Processed 674992 reads in 131.083 CPU sec, 4.184 real sec
[M::mem_process_seqs] Processed 677432 reads in 85.294 CPU sec, 2.705 real sec
[M::process] read 677362 sequences (100000184 bp) in 1.821 CPU sec, 1.909 real sec...
sorting block 157, #reads = 1077155 mem = 536878643 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282043, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 334, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (348.21, 153.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677610 sequences (100000019 bp) in 1.772 CPU sec, 1.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282435, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (344.97, 152.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676960 reads in 132.921 CPU sec, 4.222 real sec
[M::process] read 677210 sequences (100000166 bp) in 1.371 CPU sec, 1.380 real sec...
[M::mem_process_seqs] Processed 677362 reads in 86.261 CPU sec, 2.725 real sec
sorting block 158, #reads = 1077972 mem = 536877457 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282763, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (345.91, 153.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676680 sequences (100000037 bp) in 2.037 CPU sec, 2.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281932, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (346.24, 152.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676622 sequences (100000220 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_process_seqs] Processed 677610 reads in 134.208 CPU sec, 4.271 real sec
[M::mem_process_seqs] Processed 677210 reads in 85.625 CPU sec, 2.725 real sec
sorting block 159, #reads = 1076743 mem = 536877991 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282577, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 337, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (351.81, 155.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676518 sequences (100000124 bp) in 1.967 CPU sec, 2.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282008, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 340, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (353.99, 157.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676098 sequences (100000251 bp) in 1.410 CPU sec, 1.404 real sec...
[M::mem_process_seqs] Processed 676680 reads in 128.964 CPU sec, 4.101 real sec
sorting block 160, #reads = 1077257 mem = 536879621 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281605, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 340, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (355.11, 157.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674698 sequences (100000160 bp) in 1.746 CPU sec, 1.755 real sec...
[M::mem_process_seqs] Processed 676518 reads in 72.304 CPU sec, 2.288 real sec
sorting block 161, #reads = 1077591 mem = 536881671 peak = 1148858368
[M::mem_process_seqs] Processed 676622 reads in 173.059 CPU sec, 5.495 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282155, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (359.56, 158.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675376 sequences (100000111 bp) in 2.003 CPU sec, 2.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281297, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 358, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (369.18, 159.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676376 sequences (100000220 bp) in 1.370 CPU sec, 1.372 real sec...
[M::mem_process_seqs] Processed 676098 reads in 144.661 CPU sec, 4.593 real sec
sorting block 162, #reads = 1077695 mem = 536877861 peak = 1148858368
[M::mem_process_seqs] Processed 674698 reads in 85.592 CPU sec, 2.715 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281407, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (363.90, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 163, #reads = 1075744 mem = 536878466 peak = 1148858368
[M::process] read 675938 sequences (100000291 bp) in 2.464 CPU sec, 2.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282230, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 340, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (354.61, 156.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675376 reads in 129.756 CPU sec, 4.145 real sec
[M::mem_process_seqs] Processed 676376 reads in 84.273 CPU sec, 2.670 real sec
[M::process] read 676272 sequences (100000069 bp) in 2.120 CPU sec, 2.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281454, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 342, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (356.17, 156.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 164, #reads = 1077267 mem = 536880131 peak = 1148858368
[M::process] read 675822 sequences (100000066 bp) in 1.858 CPU sec, 1.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282245, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (355.63, 156.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675938 reads in 132.973 CPU sec, 4.248 real sec
[M::mem_process_seqs] Processed 676272 reads in 86.762 CPU sec, 2.738 real sec
[M::process] read 675492 sequences (100000260 bp) in 1.595 CPU sec, 1.634 real sec...
sorting block 165, #reads = 1077108 mem = 536879680 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281505, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (359.11, 158.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675382 sequences (100000131 bp) in 2.035 CPU sec, 2.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282053, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (360.84, 158.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675376 sequences (100000228 bp) in 1.380 CPU sec, 1.386 real sec...
[M::mem_process_seqs] Processed 675822 reads in 132.362 CPU sec, 4.208 real sec
[M::mem_process_seqs] Processed 675492 reads in 84.931 CPU sec, 2.691 real sec
sorting block 166, #reads = 1077159 mem = 536881681 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281880, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (365.66, 160.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675318 sequences (100000002 bp) in 2.267 CPU sec, 2.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281639, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (364.32, 160.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675382 reads in 134.804 CPU sec, 4.281 real sec
[M::process] read 675550 sequences (100000183 bp) in 1.670 CPU sec, 1.680 real sec...
sorting block 167, #reads = 1076415 mem = 536878431 peak = 1148858368
[M::mem_process_seqs] Processed 675376 reads in 86.858 CPU sec, 2.743 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281736, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (364.34, 160.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 168, #reads = 1076583 mem = 536880556 peak = 1148858368
[M::process] read 675574 sequences (100000291 bp) in 2.615 CPU sec, 2.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281607, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 350, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (365.04, 161.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675318 reads in 137.319 CPU sec, 4.370 real sec
[M::process] read 675470 sequences (100000252 bp) in 1.690 CPU sec, 1.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281968, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 350, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (366.50, 164.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675404 sequences (100000152 bp) in 1.560 CPU sec, 1.562 real sec...
[M::mem_process_seqs] Processed 675574 reads in 76.211 CPU sec, 2.414 real sec
sorting block 169, #reads = 1074890 mem = 536877247 peak = 1148858368
[M::mem_process_seqs] Processed 675550 reads in 186.692 CPU sec, 5.921 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282051, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 349, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (364.39, 162.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 170, #reads = 1074026 mem = 536879039 peak = 1148858368
[M::process] read 675806 sequences (100000180 bp) in 2.118 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281601, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 352, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (367.48, 163.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675906 sequences (100000012 bp) in 1.440 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 675470 reads in 151.755 CPU sec, 4.819 real sec
[M::mem_process_seqs] Processed 675404 reads in 89.311 CPU sec, 2.830 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282451, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (364.74, 163.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 171, #reads = 1074026 mem = 536877234 peak = 1148858368
[M::process] read 676262 sequences (100000228 bp) in 2.455 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281898, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 345, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (362.06, 163.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675806 reads in 137.694 CPU sec, 4.383 real sec
[M::process] read 676404 sequences (100000267 bp) in 1.618 CPU sec, 1.637 real sec...
[M::mem_process_seqs] Processed 675906 reads in 90.908 CPU sec, 2.874 real sec
sorting block 172, #reads = 1074801 mem = 536881664 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282180, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (357.21, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679564 sequences (100000269 bp) in 2.257 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281944, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (357.80, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679258 sequences (100000026 bp) in 1.480 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 676262 reads in 148.518 CPU sec, 4.726 real sec
[M::mem_process_seqs] Processed 676404 reads in 99.567 CPU sec, 3.155 real sec
sorting block 173, #reads = 1072904 mem = 536880385 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283230, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 323, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (338.48, 152.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678674 sequences (100000038 bp) in 2.132 CPU sec, 2.221 real sec...
[M::process] read 678708 sequences (100000212 bp) in 1.510 CPU sec, 1.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283200, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (339.58, 149.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679564 reads in 154.043 CPU sec, 4.895 real sec
sorting block 174, #reads = 1067226 mem = 536877671 peak = 1148858368
[M::mem_process_seqs] Processed 679258 reads in 99.101 CPU sec, 3.154 real sec
sorting block 175, #reads = 1069055 mem = 536877384 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283510, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (341.10, 149.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679272 sequences (100000148 bp) in 2.176 CPU sec, 2.269 real sec...
[M::process] read 679130 sequences (100000212 bp) in 1.490 CPU sec, 1.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282696, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (339.78, 149.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678674 reads in 151.867 CPU sec, 4.829 real sec
[M::mem_process_seqs] Processed 678708 reads in 97.939 CPU sec, 3.111 real sec
sorting block 176, #reads = 1069493 mem = 536878887 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283126, 13, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (334.00, 147.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (30, 1190, 6859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20517)
[M::mem_pestat] mean and std.dev: (3280.31, 3326.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 27346)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676648 sequences (100000276 bp) in 2.491 CPU sec, 2.704 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283303, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (335.17, 147.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676712 sequences (100000288 bp) in 1.500 CPU sec, 1.507 real sec...
[M::mem_process_seqs] Processed 679272 reads in 147.671 CPU sec, 4.686 real sec
[M::mem_process_seqs] Processed 679130 reads in 95.476 CPU sec, 3.030 real sec
sorting block 177, #reads = 1071021 mem = 536879559 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282434, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 348, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (356.67, 153.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679140 sequences (100000254 bp) in 1.938 CPU sec, 2.009 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282528, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 346, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (355.19, 153.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678580 sequences (100000079 bp) in 1.460 CPU sec, 1.462 real sec...
[M::mem_process_seqs] Processed 676648 reads in 144.389 CPU sec, 4.603 real sec
[M::mem_process_seqs] Processed 676712 reads in 93.070 CPU sec, 2.954 real sec
sorting block 178, #reads = 1071868 mem = 536884748 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282833, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (338.59, 151.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679056 sequences (100000110 bp) in 1.950 CPU sec, 2.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283280, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (343.61, 153.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679200 sequences (100000242 bp) in 1.500 CPU sec, 1.505 real sec...
[M::mem_process_seqs] Processed 679140 reads in 142.569 CPU sec, 4.541 real sec
sorting block 179, #reads = 1071585 mem = 536879348 peak = 1148858368
[M::mem_process_seqs] Processed 678580 reads in 90.953 CPU sec, 2.884 real sec
sorting block 180, #reads = 1074432 mem = 536878698 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283071, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (337.95, 151.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678622 sequences (100000206 bp) in 2.134 CPU sec, 2.210 real sec...
[M::process] read 678038 sequences (100000204 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282787, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 320, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (333.22, 147.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679056 reads in 143.002 CPU sec, 4.550 real sec
[M::mem_process_seqs] Processed 679200 reads in 93.030 CPU sec, 2.955 real sec
sorting block 181, #reads = 1075309 mem = 536877881 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283038, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 321, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (333.22, 145.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677464 sequences (100000283 bp) in 2.158 CPU sec, 2.225 real sec...
[M::process] read 676680 sequences (100000059 bp) in 1.470 CPU sec, 1.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282340, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (339.77, 148.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678622 reads in 140.738 CPU sec, 4.470 real sec
[M::mem_process_seqs] Processed 678038 reads in 89.741 CPU sec, 2.855 real sec
sorting block 182, #reads = 1073924 mem = 536878560 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282287, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (342.54, 149.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675738 sequences (100000242 bp) in 2.189 CPU sec, 2.281 real sec...
[M::process] read 676806 sequences (100000156 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281590, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 339, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (350.62, 151.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677464 reads in 141.218 CPU sec, 4.487 real sec
[M::mem_process_seqs] Processed 676680 reads in 91.790 CPU sec, 2.917 real sec
sorting block 183, #reads = 1074873 mem = 536877074 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281356, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (355.96, 152.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677040 sequences (100000071 bp) in 2.012 CPU sec, 2.074 real sec...
[M::process] read 677080 sequences (100000008 bp) in 1.540 CPU sec, 1.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282336, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 334, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (346.85, 150.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675738 reads in 142.710 CPU sec, 4.535 real sec
sorting block 184, #reads = 1074205 mem = 536878849 peak = 1148858368
[M::mem_process_seqs] Processed 676806 reads in 92.282 CPU sec, 2.930 real sec
sorting block 185, #reads = 1074068 mem = 536880297 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282726, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (348.20, 152.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (314, 331, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (280, 365)
[M::mem_pestat] mean and std.dev: (326.36, 7.57)
[M::mem_pestat] low and high boundaries for proper pairs: (263, 382)
[M::mem_pestat] skip orientation RR
[M::process] read 675834 sequences (100000204 bp) in 2.244 CPU sec, 2.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281826, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 332, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (345.86, 150.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676420 sequences (100000032 bp) in 1.560 CPU sec, 1.560 real sec...
[M::mem_process_seqs] Processed 677040 reads in 144.762 CPU sec, 4.599 real sec
[M::mem_process_seqs] Processed 677080 reads in 92.484 CPU sec, 2.935 real sec
sorting block 186, #reads = 1076067 mem = 536879967 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281757, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (355.80, 153.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676546 sequences (100000175 bp) in 2.695 CPU sec, 2.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281937, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (351.18, 153.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675834 reads in 144.280 CPU sec, 4.574 real sec
[M::process] read 676148 sequences (100000164 bp) in 1.585 CPU sec, 1.587 real sec...
[M::mem_process_seqs] Processed 676420 reads in 91.947 CPU sec, 2.908 real sec
sorting block 187, #reads = 1075535 mem = 536877889 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282230, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 337, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (350.03, 152.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675704 sequences (100000098 bp) in 2.371 CPU sec, 2.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281659, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 341, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (354.43, 154.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676546 reads in 142.669 CPU sec, 4.531 real sec
[M::process] read 675942 sequences (100000142 bp) in 1.800 CPU sec, 1.817 real sec...
[M::mem_process_seqs] Processed 676148 reads in 93.027 CPU sec, 2.939 real sec
sorting block 188, #reads = 1076131 mem = 536879346 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281693, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (359.63, 157.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 189, #reads = 1074883 mem = 536878564 peak = 1148858368
[M::process] read 675764 sequences (100000149 bp) in 2.188 CPU sec, 2.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281473, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 340, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (354.29, 154.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675514 sequences (100000104 bp) in 1.500 CPU sec, 1.508 real sec...
[M::mem_process_seqs] Processed 675704 reads in 141.623 CPU sec, 4.504 real sec
[M::mem_process_seqs] Processed 675942 reads in 90.529 CPU sec, 2.869 real sec
sorting block 190, #reads = 1076490 mem = 536881399 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281784, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (357.25, 155.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675588 sequences (100000280 bp) in 2.086 CPU sec, 2.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281926, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (359.58, 157.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675618 sequences (100000077 bp) in 1.510 CPU sec, 1.507 real sec...
[M::mem_process_seqs] Processed 675764 reads in 140.622 CPU sec, 4.472 real sec
[M::mem_process_seqs] Processed 675514 reads in 90.658 CPU sec, 2.870 real sec
sorting block 191, #reads = 1076184 mem = 536881224 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281291, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (358.30, 157.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675508 sequences (100000107 bp) in 2.109 CPU sec, 2.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281126, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (360.97, 159.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674970 sequences (100000250 bp) in 1.510 CPU sec, 1.513 real sec...
[M::mem_process_seqs] Processed 675588 reads in 141.238 CPU sec, 4.491 real sec
[M::mem_process_seqs] Processed 675618 reads in 90.626 CPU sec, 2.873 real sec
sorting block 192, #reads = 1075863 mem = 536881167 peak = 1148858368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281449, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (362.08, 160.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675514 sequences (100000195 bp) in 2.454 CPU sec, 2.622 real sec...
[M::process] read 450874 sequences (66662941 bp) in 1.090 CPU sec, 1.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281204, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (365.51, 160.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675508 reads in 140.018 CPU sec, 4.453 real sec
sorting block 193, #reads = 1075033 mem = 536879545 peak = 1148858368
[M::mem_process_seqs] Processed 674970 reads in 89.258 CPU sec, 2.841 real sec
sorting block 194, #reads = 1075822 mem = 536880101 peak = 1184075776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281709, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (362.12, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 188075, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (357.09, 161.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675514 reads in 117.238 CPU sec, 3.814 real sec
[M::mem_process_seqs] Processed 450874 reads in 64.500 CPU sec, 2.100 real sec
sorting block 195, #reads = 1075763 mem = 536877026 peak = 1184075776
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 210471268
overall: 69709598720 mem 26994.567 user 589.247 sys 938.618 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_bR4-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tLB:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 938.653 sec; CPU: 27583.816 sec
sorting block 196, #reads = 132938 mem = 66716524 peak = 1184075776
uniq header count 1
merging 197 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 11280252449
output reads: 211347903
bam_mem_sort: 197 calls 101.198 user 1.922 sys 104.221 real
bam_write: 197 calls 126.490 user 5.618 sys 148.494 real
execute: 1 calls 52.698 user 2.164 sys 1013.184 real
merge_files: 1 calls 52.665 user 2.131 sys 74.337 real
parse_chunk: 3138 calls 152.968 user 35.728 sys 198.497 real
read_chunk: 8453 calls 82.670 user 58.909 sys 887.469 real
sort_block: 1 calls 57.941 user 58.368 sys 852.398 real
write_chunk: 5313 calls 52.274 user 1.735 sys 73.246 real
overall: 3360251904 mem 2956.786 user 123.099 sys 1013.207 real
ls: cannot access '*.cram': No such file or directory