cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1587.299 CPU sec, 232.679 real sec
[M::process] read 688300 sequences (100000122 bp) in 0.990 CPU sec, 1.000 real sec...
[M::process] read 686282 sequences (100000183 bp) in 1.221 CPU sec, 1.246 real sec...
[M::process] read 685318 sequences (100000086 bp) in 1.189 CPU sec, 1.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284390, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 196, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (238.44, 126.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 290, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (311.56, 155.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 279, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204)
[M::mem_pestat] mean and std.dev: (304.07, 207.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1544)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684552 sequences (100000270 bp) in 1.141 CPU sec, 1.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283711, 1, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 225, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315)
[M::mem_pestat] mean and std.dev: (388.33, 328.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1701)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 305, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (322.02, 157.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (296, 414, 859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985)
[M::mem_pestat] mean and std.dev: (536.00, 269.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2548)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283157, 5, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 609, 1629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4541)
[M::mem_pestat] mean and std.dev: (705.00, 613.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5997)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 310, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (326.97, 159.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 384, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (319.47, 92.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688300 reads in 217.928 CPU sec, 6.994 real sec
[M::mem_process_seqs] Processed 686282 reads in 143.083 CPU sec, 4.613 real sec
[M::mem_process_seqs] Processed 685318 reads in 155.098 CPU sec, 5.016 real sec
sorting block 0, #reads = 1081985 mem = 536877970 peak = 590426112
[M::process] read 683432 sequences (100000154 bp) in 1.710 CPU sec, 1.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282975, 4, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 314, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (346.20, 215.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (332.19, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 275, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (291.39, 113.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683374 sequences (100000268 bp) in 1.256 CPU sec, 1.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282591, 3, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (264, 315, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (86, 531)
[M::mem_pestat] mean and std.dev: (312.86, 107.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 741)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (336.38, 161.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 223, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (262.39, 136.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 906)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682476 sequences (100000008 bp) in 1.150 CPU sec, 1.143 real sec...
[M::mem_process_seqs] Processed 684552 reads in 135.988 CPU sec, 4.330 real sec
sorting block 1, #reads = 1079870 mem = 536879844 peak = 802762752
[M::mem_process_seqs] Processed 683432 reads in 84.737 CPU sec, 2.682 real sec
sorting block 2, #reads = 1081051 mem = 536881684 peak = 918867968
[M::process] read 682002 sequences (100000085 bp) in 1.520 CPU sec, 1.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282228, 8, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 1004, 2853)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8109)
[M::mem_pestat] mean and std.dev: (2074.05, 2121.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10737)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (338.52, 162.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (343.56, 127.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682042 sequences (100000047 bp) in 1.280 CPU sec, 1.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282035, 10, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 287, 977)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2463)
[M::mem_pestat] mean and std.dev: (449.46, 364.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3206)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (338.81, 160.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (36, 459, 622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794)
[M::mem_pestat] mean and std.dev: (365.33, 373.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2380)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 246, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441)
[M::mem_pestat] mean and std.dev: (220.79, 56.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 530)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282158, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724)
[M::mem_pestat] mean and std.dev: (415.13, 353.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2229)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 326, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (338.15, 158.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 271, 746)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1806)
[M::mem_pestat] mean and std.dev: (503.60, 367.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2336)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683374 reads in 199.231 CPU sec, 6.383 real sec
[M::mem_process_seqs] Processed 682476 reads in 146.514 CPU sec, 4.722 real sec
[M::mem_process_seqs] Processed 682002 reads in 158.251 CPU sec, 5.114 real sec
sorting block 3, #reads = 1079381 mem = 536880309 peak = 918867968
[M::process] read 682308 sequences (100000081 bp) in 1.995 CPU sec, 2.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281696, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 324, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (265.00, 108.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 324, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (337.30, 159.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 406, 918)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2434)
[M::mem_pestat] mean and std.dev: (657.18, 734.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3594)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 4, #reads = 1078836 mem = 536880026 peak = 918867968
[M::process] read 681944 sequences (100000274 bp) in 1.491 CPU sec, 1.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282613, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 295, 1355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3619)
[M::mem_pestat] mean and std.dev: (704.20, 804.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4751)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 322, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (335.90, 158.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 431, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296)
[M::mem_pestat] mean and std.dev: (368.65, 146.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1638)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682042 reads in 147.227 CPU sec, 4.689 real sec
[M::process] read 686322 sequences (100000154 bp) in 1.388 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 682308 reads in 93.853 CPU sec, 2.960 real sec
sorting block 5, #reads = 1077130 mem = 536880147 peak = 918867968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282191, 4, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 261, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (253.54, 110.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 939)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (342.07, 163.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 363, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212)
[M::mem_pestat] mean and std.dev: (301.09, 120.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1541)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686134 sequences (100000093 bp) in 1.686 CPU sec, 1.784 real sec...
[M::process] read 685300 sequences (100000144 bp) in 1.353 CPU sec, 1.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284057, 9, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (240, 409, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095)
[M::mem_pestat] mean and std.dev: (396.52, 198.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1380)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 304, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (323.74, 160.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 337, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (357.12, 219.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1346)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681944 reads in 143.709 CPU sec, 4.593 real sec
[M::mem_process_seqs] Processed 686322 reads in 89.265 CPU sec, 2.842 real sec
sorting block 6, #reads = 1077331 mem = 536878439 peak = 918867968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283933, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 187, 268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 440)
[M::mem_pestat] mean and std.dev: (185.22, 59.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 526)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 305, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (322.98, 158.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 327, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (317.90, 141.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684676 sequences (100000141 bp) in 1.728 CPU sec, 1.836 real sec...
[M::process] read 683750 sequences (100000221 bp) in 1.370 CPU sec, 1.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283079, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 156, 740)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1950)
[M::mem_pestat] mean and std.dev: (322.85, 276.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2555)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 309, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (326.12, 159.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 305, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (358.50, 282.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1501)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686134 reads in 139.990 CPU sec, 4.470 real sec
[M::mem_process_seqs] Processed 685300 reads in 86.060 CPU sec, 2.742 real sec
sorting block 7, #reads = 1081786 mem = 536877579 peak = 918867968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 282759, 4, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 286, 599)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289)
[M::mem_pestat] mean and std.dev: (352.25, 170.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1634)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (331.78, 161.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 260, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (267.67, 138.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683574 sequences (100000201 bp) in 2.114 CPU sec, 2.222 real sec...
[M::process] read 683124 sequences (100000280 bp) in 1.500 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283146, 7, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 300, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (292.73, 168.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 318, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (332.94, 160.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 261, 667)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1633)
[M::mem_pestat] mean and std.dev: (364.95, 225.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2116)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684676 reads in 141.751 CPU sec, 4.522 real sec
sorting block 8, #reads = 1082448 mem = 536884196 peak = 918867968
[M::mem_process_seqs] Processed 683750 reads in 87.273 CPU sec, 2.778 real sec
sorting block 9, #reads = 1080834 mem = 536883115 peak = 918867968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282773, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 288, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118)
[M::mem_pestat] mean and std.dev: (337.74, 210.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1422)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (335.27, 161.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 285, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 458)
[M::mem_pestat] mean and std.dev: (260.53, 74.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 558)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682694 sequences (100000170 bp) in 1.937 CPU sec, 2.097 real sec...
[M::process] read 682624 sequences (100000292 bp) in 1.500 CPU sec, 1.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282521, 1, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (247, 253, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (314.71, 158.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (337.74, 162.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 311, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (269.75, 93.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683574 reads in 141.298 CPU sec, 4.509 real sec
[M::mem_process_seqs] Processed 683124 reads in 87.193 CPU sec, 2.767 real sec
sorting block 10, #reads = 1080351 mem = 536878902 peak = 918867968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282587, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 451, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1236)
[M::mem_pestat] mean and std.dev: (366.78, 174.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1579)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 324, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (337.57, 160.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 277, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (278.73, 102.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1330)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682536 sequences (100000176 bp) in 2.225 CPU sec, 2.347 real sec...
[M::process] read 682016 sequences (100000126 bp) in 1.400 CPU sec, 1.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282625, 3, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 251, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (281.19, 146.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (336.68, 160.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 377, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210)
[M::mem_pestat] mean and std.dev: (336.46, 147.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1544)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682694 reads in 143.536 CPU sec, 4.577 real sec
[M::mem_process_seqs] Processed 682624 reads in 88.889 CPU sec, 2.830 real sec
sorting block 11, #reads = 1078980 mem = 536878414 peak = 918867968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282655, 12, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 352, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161)
[M::mem_pestat] mean and std.dev: (371.67, 233.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1493)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 320, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (334.88, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1079, 1691, 2710)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5972)
[M::mem_pestat] mean and std.dev: (1488.50, 872.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7603)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 199, 921)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2429)
[M::mem_pestat] mean and std.dev: (490.33, 429.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3183)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684070 sequences (100000098 bp) in 1.926 CPU sec, 2.054 real sec...
[M::process] read 687058 sequences (100000086 bp) in 1.430 CPU sec, 1.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282575, 1, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 184, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349)
[M::mem_pestat] mean and std.dev: (228.14, 143.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1748)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (340.26, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 278, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (279.69, 96.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682536 reads in 142.055 CPU sec, 4.535 real sec
sorting block 12, #reads = 1079902 mem = 536877916 peak = 918867968
[M::mem_process_seqs] Processed 682016 reads in 88.195 CPU sec, 2.811 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283940, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 226, 880)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2278)
[M::mem_pestat] mean and std.dev: (488.92, 526.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2977)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (334.09, 164.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 277, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (323.11, 151.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 13, #reads = 1077426 mem = 536879577 peak = 918867968
[M::process] read 684928 sequences (100000097 bp) in 2.188 CPU sec, 2.310 real sec...
[M::process] read 683962 sequences (100000125 bp) in 1.437 CPU sec, 1.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284617, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 294, 620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1534)
[M::mem_pestat] mean and std.dev: (277.95, 163.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1991)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 299, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (319.18, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 300, 814)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2002)
[M::mem_pestat] mean and std.dev: (409.75, 259.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2596)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684070 reads in 138.762 CPU sec, 4.422 real sec
[M::mem_process_seqs] Processed 687058 reads in 86.385 CPU sec, 2.747 real sec
sorting block 14, #reads = 1081083 mem = 536878193 peak = 918867968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283478, 5, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 304, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (269.05, 100.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 311, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (328.45, 160.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 346, 615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339)
[M::mem_pestat] mean and std.dev: (422.09, 252.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1701)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683468 sequences (100000030 bp) in 1.926 CPU sec, 2.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283407, 1, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 260, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1479)
[M::mem_pestat] mean and std.dev: (488.30, 470.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2368)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (335.45, 163.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 223, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122)
[M::mem_pestat] mean and std.dev: (231.89, 117.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1436)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683392 sequences (100000281 bp) in 1.430 CPU sec, 1.421 real sec...
[M::mem_process_seqs] Processed 684928 reads in 137.498 CPU sec, 4.396 real sec
[M::mem_process_seqs] Processed 683962 reads in 86.303 CPU sec, 2.734 real sec
sorting block 15, #reads = 1081244 mem = 536881277 peak = 918867968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283033, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 275, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (291.39, 86.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (339.82, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 243, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (239.30, 98.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682594 sequences (100000047 bp) in 2.277 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282692, 13, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 371, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (366.93, 245.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (338.38, 165.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1103, 1334, 2952)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6650)
[M::mem_pestat] mean and std.dev: (1545.46, 828.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8499)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 206, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532)
[M::mem_pestat] mean and std.dev: (225.15, 103.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 655)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682226 sequences (100000035 bp) in 1.400 CPU sec, 1.400 real sec...
[M::mem_process_seqs] Processed 683468 reads in 140.564 CPU sec, 4.481 real sec
[M::mem_process_seqs] Processed 683392 reads in 88.571 CPU sec, 2.812 real sec
sorting block 16, #reads = 1080037 mem = 536879556 peak = 918867968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282509, 1, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 261, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (274.13, 208.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1335)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (341.11, 165.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 348, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (388.21, 268.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1464)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682474 sequences (100000257 bp) in 2.007 CPU sec, 2.147 real sec...
[M::process] read 682524 sequences (100000248 bp) in 1.390 CPU sec, 1.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282501, 10, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 385, 1020)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2596)
[M::mem_pestat] mean and std.dev: (479.38, 316.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3384)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (342.83, 165.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (769, 1834, 2415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5707)
[M::mem_pestat] mean and std.dev: (1646.56, 1089.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7353)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 233, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223)
[M::mem_pestat] mean and std.dev: (306.42, 172.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1559)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682594 reads in 142.683 CPU sec, 4.570 real sec
sorting block 17, #reads = 1079229 mem = 536877834 peak = 918867968
[M::mem_process_seqs] Processed 682226 reads in 86.633 CPU sec, 2.753 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282699, 11, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 207, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553)
[M::mem_pestat] mean and std.dev: (208.07, 49.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 324, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (339.43, 163.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (463, 581, 1072)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2290)
[M::mem_pestat] mean and std.dev: (687.60, 513.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2899)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 292, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 512)
[M::mem_pestat] mean and std.dev: (262.37, 69.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 608)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1079126 mem = 536878960 peak = 954650624
[M::process] read 682156 sequences (100000096 bp) in 2.280 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282916, 3, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 263, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (58, 518)
[M::mem_pestat] mean and std.dev: (257.62, 90.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 618)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (339.27, 165.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 213, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (246.24, 149.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682332 sequences (100000121 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_process_seqs] Processed 682474 reads in 135.987 CPU sec, 4.320 real sec
[M::mem_process_seqs] Processed 682524 reads in 84.839 CPU sec, 2.683 real sec
sorting block 19, #reads = 1078548 mem = 536880033 peak = 954650624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282806, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 392, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118)
[M::mem_pestat] mean and std.dev: (367.59, 157.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (341.45, 164.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 328, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112)
[M::mem_pestat] mean and std.dev: (347.86, 213.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1419)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687740 sequences (100000298 bp) in 1.924 CPU sec, 2.026 real sec...
[M::process] read 684696 sequences (100000301 bp) in 1.370 CPU sec, 1.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282967, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 300, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (12, 592)
[M::mem_pestat] mean and std.dev: (271.91, 87.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (344.72, 167.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (303, 405, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 684)
[M::mem_pestat] mean and std.dev: (383.74, 130.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682156 reads in 135.329 CPU sec, 4.305 real sec
[M::mem_process_seqs] Processed 682332 reads in 86.596 CPU sec, 2.743 real sec
sorting block 20, #reads = 1077484 mem = 536876668 peak = 954650624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284483, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 224, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (216.33, 75.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 294, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (317.64, 161.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683952 sequences (100000173 bp) in 2.370 CPU sec, 2.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283612, 0, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (332.52, 164.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 426, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (359.94, 124.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RR
[M::process] read 683084 sequences (100000249 bp) in 1.390 CPU sec, 1.391 real sec...
[M::mem_process_seqs] Processed 687740 reads in 133.366 CPU sec, 4.231 real sec
[M::mem_process_seqs] Processed 684696 reads in 83.900 CPU sec, 2.652 real sec
sorting block 21, #reads = 1079184 mem = 536878253 peak = 954650624
[M::process] read 682688 sequences (100000183 bp) in 1.992 CPU sec, 2.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282546, 0, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 250, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (221.40, 71.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 719)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (343.32, 168.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 312, 614)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458)
[M::mem_pestat] mean and std.dev: (458.95, 347.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1880)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682894 sequences (100000138 bp) in 1.380 CPU sec, 1.376 real sec...
[M::mem_process_seqs] Processed 683084 reads in 77.547 CPU sec, 2.454 real sec
sorting block 22, #reads = 1082629 mem = 536878281 peak = 954650624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283188, 1, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 405, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270)
[M::mem_pestat] mean and std.dev: (356.06, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1635)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (338.07, 166.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 265, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (319.00, 211.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283036, 4, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 326, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339)
[M::mem_pestat] mean and std.dev: (295.20, 135.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1721)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (343.41, 166.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (289, 300, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 655)
[M::mem_pestat] mean and std.dev: (279.87, 87.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 777)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683952 reads in 204.244 CPU sec, 6.502 real sec
[M::process] read 681974 sequences (100000049 bp) in 1.617 CPU sec, 1.636 real sec...
[M::mem_process_seqs] Processed 682688 reads in 95.705 CPU sec, 3.048 real sec
sorting block 23, #reads = 1081295 mem = 536879590 peak = 955994112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282520, 1, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 258, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261)
[M::mem_pestat] mean and std.dev: (319.86, 168.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1620)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (341.88, 166.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 246, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (267.40, 72.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682290 sequences (100000003 bp) in 2.364 CPU sec, 2.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282510, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 270, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (248.07, 85.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 741)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (340.87, 163.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 291, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320)
[M::mem_pestat] mean and std.dev: (385.46, 233.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1667)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682030 sequences (100000105 bp) in 1.360 CPU sec, 1.358 real sec...
[M::mem_process_seqs] Processed 682894 reads in 135.876 CPU sec, 4.315 real sec
sorting block 24, #reads = 1080569 mem = 536878532 peak = 955994112
[M::mem_process_seqs] Processed 681974 reads in 85.775 CPU sec, 2.716 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282688, 2, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (337.25, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 295, 780)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1830)
[M::mem_pestat] mean and std.dev: (432.50, 324.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2355)
[M::mem_pestat] skip orientation RR
sorting block 25, #reads = 1078965 mem = 536878988 peak = 955994112
[M::process] read 681862 sequences (100000110 bp) in 2.216 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282202, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 355, 1175)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3055)
[M::mem_pestat] mean and std.dev: (473.50, 377.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3995)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (338.85, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 250, 657)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1523)
[M::mem_pestat] mean and std.dev: (401.95, 244.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1956)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681628 sequences (100000179 bp) in 1.380 CPU sec, 1.384 real sec...
[M::mem_process_seqs] Processed 682290 reads in 135.695 CPU sec, 4.306 real sec
[M::mem_process_seqs] Processed 682030 reads in 86.043 CPU sec, 2.723 real sec
sorting block 26, #reads = 1078419 mem = 536878526 peak = 955994112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281969, 12, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 404, 902)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2274)
[M::mem_pestat] mean and std.dev: (563.36, 467.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2960)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (341.30, 163.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (439, 618, 1279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2959)
[M::mem_pestat] mean and std.dev: (827.92, 654.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3799)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 454, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269)
[M::mem_pestat] mean and std.dev: (349.17, 165.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1618)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 688212 sequences (100000046 bp) in 2.203 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282020, 1, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 220, 1073)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3011)
[M::mem_pestat] mean and std.dev: (429.30, 424.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3980)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (346.89, 166.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 315, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358)
[M::mem_pestat] mean and std.dev: (353.59, 257.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1761)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 689162 sequences (100000282 bp) in 1.430 CPU sec, 1.437 real sec...
[M::mem_process_seqs] Processed 681862 reads in 135.347 CPU sec, 4.295 real sec
[M::mem_process_seqs] Processed 681628 reads in 85.229 CPU sec, 2.699 real sec
sorting block 27, #reads = 1078008 mem = 536881430 peak = 955994112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284860, 1, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 258, 623)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401)
[M::mem_pestat] mean and std.dev: (368.16, 276.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1790)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 293, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (317.27, 161.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 234, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690)
[M::mem_pestat] mean and std.dev: (268.17, 92.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686886 sequences (100000187 bp) in 2.210 CPU sec, 2.318 real sec...
[M::process] read 685972 sequences (100000155 bp) in 1.400 CPU sec, 1.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 285482, 4, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 330, 636)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1596)
[M::mem_pestat] mean and std.dev: (366.48, 232.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2076)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 288, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (312.83, 158.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328)
[M::mem_pestat] mean and std.dev: (361.86, 226.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1704)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688212 reads in 145.647 CPU sec, 4.626 real sec
[M::mem_process_seqs] Processed 689162 reads in 90.437 CPU sec, 2.867 real sec
sorting block 28, #reads = 1076908 mem = 536883389 peak = 955994112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284135, 0, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (389, 597, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (470.18, 162.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 303, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (321.88, 159.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 265, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126)
[M::mem_pestat] mean and std.dev: (253.33, 111.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1435)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685406 sequences (100000248 bp) in 2.259 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283753, 1, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 346, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (345.44, 156.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1364)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 310, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (327.35, 161.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (351.19, 199.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685130 sequences (100000009 bp) in 1.420 CPU sec, 1.416 real sec...
[M::mem_process_seqs] Processed 686886 reads in 139.318 CPU sec, 4.427 real sec
sorting block 29, #reads = 1080427 mem = 536878813 peak = 961110016
[M::mem_process_seqs] Processed 685972 reads in 87.316 CPU sec, 2.759 real sec
sorting block 30, #reads = 1079658 mem = 536881877 peak = 961110016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283775, 9, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 505, 1456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3980)
[M::mem_pestat] mean and std.dev: (700.83, 716.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5242)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 312, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (329.18, 161.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 337, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (301.11, 136.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684844 sequences (100000217 bp) in 1.998 CPU sec, 2.099 real sec...
[M::process] read 684364 sequences (100000108 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 283512, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 314, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (330.14, 160.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 193, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (218.36, 86.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685406 reads in 141.369 CPU sec, 4.496 real sec
[M::mem_process_seqs] Processed 685130 reads in 89.082 CPU sec, 2.827 real sec
sorting block 31, #reads = 1079521 mem = 536878458 peak = 961110016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 283890, 4, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 188, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (253.16, 178.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 315, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (331.65, 162.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 306, 688)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1612)
[M::mem_pestat] mean and std.dev: (382.09, 218.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2074)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683522 sequences (100000005 bp) in 2.044 CPU sec, 2.132 real sec...
[M::process] read 682998 sequences (100000228 bp) in 1.400 CPU sec, 1.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 283057, 8, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169)
[M::mem_pestat] mean and std.dev: (324.48, 145.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1485)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (331.93, 160.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 270, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (301.21, 160.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684844 reads in 143.951 CPU sec, 4.582 real sec
[M::mem_process_seqs] Processed 684364 reads in 91.038 CPU sec, 2.881 real sec
sorting block 32, #reads = 1077628 mem = 536879910 peak = 961110016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 283043, 7, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 308, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (274.90, 99.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 910)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 318, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (332.16, 158.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (314, 537, 618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226)
[M::mem_pestat] mean and std.dev: (513.73, 312.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1764)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683664 sequences (100000219 bp) in 2.265 CPU sec, 2.368 real sec...
[M::process] read 695724 sequences (100000076 bp) in 1.450 CPU sec, 1.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283110, 4, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 390, 1336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3626)
[M::mem_pestat] mean and std.dev: (559.79, 465.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4771)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (336.62, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 307, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149)
[M::mem_pestat] mean and std.dev: (396.17, 208.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1460)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683522 reads in 145.897 CPU sec, 4.633 real sec
sorting block 33, #reads = 1075939 mem = 536878400 peak = 961110016
[M::mem_process_seqs] Processed 682998 reads in 91.194 CPU sec, 2.887 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282996, 6, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 302, 1073)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2795)
[M::mem_pestat] mean and std.dev: (490.95, 387.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3656)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (336.95, 163.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 336, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (337.05, 146.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 34, #reads = 1075007 mem = 536878522 peak = 969289728
[M::process] read 691814 sequences (100000031 bp) in 2.360 CPU sec, 2.468 real sec...
[M::process] read 689066 sequences (100000247 bp) in 1.450 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287196, 13, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 325, 875)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2259)
[M::mem_pestat] mean and std.dev: (499.92, 306.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2951)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 253, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (281.55, 142.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (409, 826, 836)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690)
[M::mem_pestat] mean and std.dev: (596.00, 270.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2117)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 364, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (269.40, 96.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683664 reads in 154.503 CPU sec, 4.909 real sec
[M::mem_process_seqs] Processed 695724 reads in 102.362 CPU sec, 3.245 real sec
sorting block 35, #reads = 1073423 mem = 536878777 peak = 969289728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286566, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 264, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (256.60, 159.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 273, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (297.85, 149.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 348, 852)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2130)
[M::mem_pestat] mean and std.dev: (332.27, 183.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2769)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687550 sequences (100000161 bp) in 2.252 CPU sec, 2.345 real sec...
[M::process] read 686406 sequences (100000182 bp) in 1.410 CPU sec, 1.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285839, 2, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 232, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (251.78, 81.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 287, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (308.63, 152.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 272, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (60, 500)
[M::mem_pestat] mean and std.dev: (263.69, 80.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 691814 reads in 156.846 CPU sec, 4.984 real sec
[M::mem_process_seqs] Processed 689066 reads in 100.481 CPU sec, 3.193 real sec
sorting block 36, #reads = 1074821 mem = 536877323 peak = 969289728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 285594, 8, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 252, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (272.19, 136.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 297, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (315.74, 154.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 411, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1555)
[M::mem_pestat] mean and std.dev: (458.21, 280.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2012)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685550 sequences (100000242 bp) in 2.847 CPU sec, 2.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284003, 1, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (359, 359, 782)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1628)
[M::mem_pestat] mean and std.dev: (513.60, 363.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2051)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 302, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (320.13, 156.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 320, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1580)
[M::mem_pestat] mean and std.dev: (370.54, 185.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2028)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684550 sequences (100000146 bp) in 1.640 CPU sec, 1.638 real sec...
[M::mem_process_seqs] Processed 687550 reads in 158.008 CPU sec, 5.013 real sec
[M::mem_process_seqs] Processed 686406 reads in 100.106 CPU sec, 3.166 real sec
sorting block 37, #reads = 1075152 mem = 536878921 peak = 969289728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284033, 0, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 509, 740)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1896)
[M::mem_pestat] mean and std.dev: (468.35, 384.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2474)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 310, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (327.48, 160.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 194, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (272.27, 152.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683328 sequences (100000054 bp) in 2.612 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283650, 0, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 281, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161)
[M::mem_pestat] mean and std.dev: (321.89, 143.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1475)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 316, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (334.80, 165.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 288, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1461)
[M::mem_pestat] mean and std.dev: (331.29, 189.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1873)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682784 sequences (100000127 bp) in 1.540 CPU sec, 1.539 real sec...
[M::mem_process_seqs] Processed 685550 reads in 153.928 CPU sec, 4.887 real sec
sorting block 38, #reads = 1074664 mem = 536879193 peak = 969289728
[M::mem_process_seqs] Processed 684550 reads in 96.372 CPU sec, 3.050 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282867, 2, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (356, 503, 616)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136)
[M::mem_pestat] mean and std.dev: (475.00, 127.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1396)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 323, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (339.56, 166.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (290, 406, 727)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1601)
[M::mem_pestat] mean and std.dev: (513.00, 338.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2038)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 39, #reads = 1076329 mem = 536876994 peak = 1010884608
[M::process] read 687902 sequences (100000006 bp) in 2.679 CPU sec, 2.778 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282309, 2, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (277, 464, 1655)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4411)
[M::mem_pestat] mean and std.dev: (842.94, 759.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5789)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 329, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (345.90, 170.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 417, 912)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362)
[M::mem_pestat] mean and std.dev: (492.71, 357.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3087)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687784 sequences (100000236 bp) in 1.600 CPU sec, 1.601 real sec...
[M::mem_process_seqs] Processed 683328 reads in 151.635 CPU sec, 4.813 real sec
[M::mem_process_seqs] Processed 682784 reads in 95.858 CPU sec, 3.024 real sec
sorting block 40, #reads = 1075324 mem = 536878277 peak = 1010884608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284677, 11, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 245, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (246.35, 87.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 295, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (319.99, 163.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (255, 327, 1153)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2949)
[M::mem_pestat] mean and std.dev: (628.18, 528.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3847)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 222, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (75, 360)
[M::mem_pestat] mean and std.dev: (199.80, 34.32)
[M::mem_pestat] low and high boundaries for proper pairs: (18, 417)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 686374 sequences (100000184 bp) in 2.650 CPU sec, 2.792 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284827, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 303, 809)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2039)
[M::mem_pestat] mean and std.dev: (475.65, 438.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2654)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 295, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (316.56, 158.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 297, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (330.92, 126.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685196 sequences (100000122 bp) in 1.520 CPU sec, 1.512 real sec...
[M::mem_process_seqs] Processed 687902 reads in 155.879 CPU sec, 4.951 real sec
[M::mem_process_seqs] Processed 687784 reads in 93.461 CPU sec, 2.961 real sec
sorting block 41, #reads = 1073790 mem = 536878614 peak = 1010884608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284320, 8, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 296, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (281.25, 108.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 728)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 303, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (323.36, 160.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (287, 331, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (332.90, 124.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684660 sequences (100000214 bp) in 2.714 CPU sec, 2.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283781, 1, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 194, 3590)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10472)
[M::mem_pestat] mean and std.dev: (1038.42, 1474.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13913)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 310, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (327.36, 160.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 327, 858)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2174)
[M::mem_pestat] mean and std.dev: (480.32, 376.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2832)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686374 reads in 144.939 CPU sec, 4.603 real sec
[M::process] read 685530 sequences (100000263 bp) in 1.516 CPU sec, 1.521 real sec...
[M::mem_process_seqs] Processed 685196 reads in 91.877 CPU sec, 2.902 real sec
sorting block 42, #reads = 1080228 mem = 536878457 peak = 1010884608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283493, 10, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 319, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (342.75, 155.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1371)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (331.91, 162.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (671, 850, 858)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (297, 1232)
[M::mem_pestat] mean and std.dev: (773.11, 91.38)
[M::mem_pestat] low and high boundaries for proper pairs: (110, 1419)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 273, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155)
[M::mem_pestat] mean and std.dev: (277.32, 138.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1492)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 43, #reads = 1079172 mem = 536878767 peak = 1010884608
[M::process] read 684180 sequences (100000250 bp) in 2.797 CPU sec, 2.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284274, 6, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 386, 755)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1923)
[M::mem_pestat] mean and std.dev: (434.34, 282.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2507)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 309, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (327.31, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 240, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (254.24, 116.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684660 reads in 145.603 CPU sec, 4.628 real sec
[M::process] read 683496 sequences (100000130 bp) in 1.668 CPU sec, 1.692 real sec...
[M::mem_process_seqs] Processed 685530 reads in 92.169 CPU sec, 2.908 real sec
sorting block 44, #reads = 1079639 mem = 536880532 peak = 1010884608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283481, 2, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 293, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (269.30, 133.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 316, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (334.05, 164.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 228, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370)
[M::mem_pestat] mean and std.dev: (293.29, 229.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1777)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683576 sequences (100000300 bp) in 2.544 CPU sec, 2.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282787, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 298, 1080)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2986)
[M::mem_pestat] mean and std.dev: (486.43, 469.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3939)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 322, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (339.18, 166.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 684180 reads in 147.723 CPU sec, 4.689 real sec
[M::process] read 683130 sequences (100000144 bp) in 1.917 CPU sec, 1.941 real sec...
[M::mem_process_seqs] Processed 683496 reads in 93.067 CPU sec, 2.925 real sec
sorting block 45, #reads = 1077106 mem = 536880659 peak = 1010884608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283328, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 190, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (205.60, 81.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 319, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (337.40, 165.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 337, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (292.56, 100.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 917)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682914 sequences (100000170 bp) in 2.532 CPU sec, 2.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282966, 3, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 215, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (319.42, 239.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 322, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (340.05, 166.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (284, 408, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (407.27, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683576 reads in 150.427 CPU sec, 4.786 real sec
[M::process] read 694130 sequences (100000038 bp) in 1.793 CPU sec, 1.812 real sec...
[M::mem_process_seqs] Processed 683130 reads in 96.732 CPU sec, 3.049 real sec
sorting block 46, #reads = 1076612 mem = 536879694 peak = 1010884608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282853, 4, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 193, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (240.53, 163.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (341.69, 168.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (338.44, 143.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690100 sequences (100000000 bp) in 2.704 CPU sec, 2.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 287033, 9, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 197, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203)
[M::mem_pestat] mean and std.dev: (325.59, 226.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1554)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 261, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (290.76, 149.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 353, 796)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012)
[M::mem_pestat] mean and std.dev: (434.33, 279.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2620)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687296 sequences (100000268 bp) in 1.679 CPU sec, 1.677 real sec...
[M::mem_process_seqs] Processed 682914 reads in 164.592 CPU sec, 5.222 real sec
sorting block 47, #reads = 1074738 mem = 536879754 peak = 1010884608
[M::mem_process_seqs] Processed 694130 reads in 107.504 CPU sec, 3.401 real sec
sorting block 48, #reads = 1070801 mem = 536880273 peak = 1010884608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285879, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 259, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (271.27, 116.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 284, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (306.85, 153.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 192, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (222.83, 81.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685766 sequences (100000111 bp) in 2.670 CPU sec, 2.785 real sec...
[M::process] read 685006 sequences (100000047 bp) in 1.510 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284697, 7, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (296, 333, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (222, 407)
[M::mem_pestat] mean and std.dev: (325.43, 12.52)
[M::mem_pestat] low and high boundaries for proper pairs: (185, 444)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 300, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (319.00, 157.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 313, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1119)
[M::mem_pestat] mean and std.dev: (340.11, 231.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1452)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690100 reads in 159.120 CPU sec, 5.048 real sec
[M::mem_process_seqs] Processed 687296 reads in 99.999 CPU sec, 3.168 real sec
sorting block 49, #reads = 1073603 mem = 536878965 peak = 1010884608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284129, 6, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 255, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 432)
[M::mem_pestat] mean and std.dev: (226.63, 57.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 310, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (326.51, 159.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 248, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (237.62, 92.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684026 sequences (100000229 bp) in 2.759 CPU sec, 2.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284226, 6, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (288, 307, 1351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3477)
[M::mem_pestat] mean and std.dev: (819.60, 960.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4663)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 314, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (331.70, 162.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 341, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1147)
[M::mem_pestat] mean and std.dev: (369.21, 202.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1458)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683274 sequences (100000227 bp) in 1.500 CPU sec, 1.502 real sec...
[M::mem_process_seqs] Processed 685766 reads in 156.869 CPU sec, 4.979 real sec
[M::mem_process_seqs] Processed 685006 reads in 98.890 CPU sec, 3.131 real sec
sorting block 50, #reads = 1072689 mem = 536881141 peak = 1010884608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284353, 4, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 271, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 459)
[M::mem_pestat] mean and std.dev: (255.82, 71.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 543)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (334.89, 162.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 428, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177)
[M::mem_pestat] mean and std.dev: (458.05, 310.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1700)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682640 sequences (100000018 bp) in 2.560 CPU sec, 2.691 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283306, 3, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 217, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (231.85, 83.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 682216 sequences (100000085 bp) in 1.450 CPU sec, 1.455 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (201, 319, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (336.20, 163.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 372, 696)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1696)
[M::mem_pestat] mean and std.dev: (387.26, 259.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2196)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684026 reads in 153.363 CPU sec, 4.870 real sec
sorting block 51, #reads = 1074452 mem = 536879492 peak = 1010884608
[M::mem_process_seqs] Processed 683274 reads in 95.351 CPU sec, 3.021 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283529, 10, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 358, 906)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2310)
[M::mem_pestat] mean and std.dev: (527.78, 326.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3012)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (338.08, 163.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 186, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (193.44, 105.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 428, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (376.78, 167.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 52, #reads = 1074854 mem = 536878625 peak = 1010884608
[M::process] read 650990 sequences (95448630 bp) in 2.069 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282656, 4, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 215, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (232.21, 109.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1371)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 326, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (343.09, 166.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 302, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (337.72, 186.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682640 reads in 146.246 CPU sec, 4.705 real sec
[M::mem_process_seqs] Processed 682216 reads in 91.952 CPU sec, 2.964 real sec
sorting block 53, #reads = 1075756 mem = 536877714 peak = 1010884608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 269900, 6, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 332, 889)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2281)
[M::mem_pestat] mean and std.dev: (538.37, 395.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2977)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (345.94, 168.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 304, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (294.70, 126.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 650990 reads in 76.776 CPU sec, 2.549 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 58847920
overall: 69885956096 mem 8075.849 user 287.873 sys 456.716 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 456.752 sec; CPU: 8363.725 sec
sorting block 54, #reads = 951347 mem = 474955079 peak = 1010884608
uniq header count 1
merging 55 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3603288108
output reads: 59162719
bam_mem_sort: 55 calls 23.625 user 0.287 sys 24.015 real
bam_write: 55 calls 19.016 user 0.649 sys 19.739 real
execute: 1 calls 14.969 user 0.572 sys 477.807 real
merge_files: 1 calls 14.952 user 0.548 sys 20.341 real
parse_chunk: 879 calls 31.981 user 8.344 sys 40.934 real
read_chunk: 2372 calls 19.510 user 19.040 sys 223.590 real
sort_block: 1 calls 16.161 user 18.551 sys 216.773 real
write_chunk: 1491 calls 14.586 user 0.375 sys 19.624 real
overall: 2677948416 mem 775.112 user 35.640 sys 477.828 real
ls: cannot access '*.cram': No such file or directory