cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_27F-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. No more license available for sentieon:klib version: sentieon-genomics-202308.03 No more license available for sentieon:klib [M::bwa_idx_load_from_disk] read 3171 ALT contigs No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib No more license available for sentieon:klib Received only 12 licenses out of 31 requested for sentieon:klib Received only 12 licenses out of 31 requested for sentieon:klib Received only 12 licenses out of 31 requested for sentieon:klib [M::main_mem] bwa index loaded in 1612.899 CPU sec, 739.551 real sec [M::process] read 700864 sequences (100000002 bp) in 0.976 CPU sec, 0.986 real sec... [M::process] read 700598 sequences (100000073 bp) in 1.079 CPU sec, 1.086 real sec... [M::process] read 700144 sequences (100000127 bp) in 1.118 CPU sec, 1.142 real sec... [M::process] read 699402 sequences (100000038 bp) in 1.128 CPU sec, 1.150 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 292113, 28, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 409, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2138) [M::mem_pestat] mean and std.dev: (552.37, 422.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (259.37, 129.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (255, 926, 2739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7707) [M::mem_pestat] mean and std.dev: (1536.64, 1801.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10191) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 315, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373) [M::mem_pestat] mean and std.dev: (361.00, 268.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 292207, 28, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (245.98, 165.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (260.66, 130.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (408, 848, 1803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4593) [M::mem_pestat] mean and std.dev: (998.96, 839.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 221, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (271.50, 187.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 292077, 19, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 243, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (260.61, 158.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (263.75, 132.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (322, 542, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1759) [M::mem_pestat] mean and std.dev: (494.18, 316.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 294, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1307) [M::mem_pestat] mean and std.dev: (341.75, 242.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700864 reads in 190.670 CPU sec, 15.820 real sec [M::mem_process_seqs] Processed 700598 reads in 138.374 CPU sec, 11.656 real sec sorting block 0, #reads = 1090828 mem = 536879348 peak = 615956480 [M::mem_process_seqs] Processed 700144 reads in 150.776 CPU sec, 12.648 real sec [M::process] read 697098 sequences (100000085 bp) in 1.638 CPU sec, 1.725 real sec... [M::process] read 696454 sequences (100000256 bp) in 1.435 CPU sec, 1.484 real sec... [M::process] read 696134 sequences (100000196 bp) in 1.140 CPU sec, 1.140 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 291771, 20, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 274, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744) [M::mem_pestat] mean and std.dev: (351.78, 304.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (264.72, 133.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (129, 684, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3654) [M::mem_pestat] mean and std.dev: (769.05, 709.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4829) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 231, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (269.06, 175.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 290380, 24, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 271, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (255.57, 128.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (272.98, 137.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (373, 963, 2155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5719) [M::mem_pestat] mean and std.dev: (1224.62, 1149.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 366, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (351.06, 165.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 290558, 21, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 314, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (311.38, 154.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (274.06, 136.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (208, 1035, 2017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5635) [M::mem_pestat] mean and std.dev: (1115.71, 964.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7444) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 354, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1649) [M::mem_pestat] mean and std.dev: (417.73, 303.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699402 reads in 189.332 CPU sec, 15.582 real sec sorting block 1, #reads = 1091332 mem = 536879052 peak = 873906176 [M::mem_process_seqs] Processed 697098 reads in 138.887 CPU sec, 11.561 real sec sorting block 2, #reads = 1089871 mem = 536878120 peak = 890007552 [M::mem_process_seqs] Processed 696454 reads in 151.467 CPU sec, 12.570 real sec [M::process] read 695178 sequences (100000039 bp) in 1.584 CPU sec, 1.665 real sec... [M::process] read 694108 sequences (100000250 bp) in 1.718 CPU sec, 1.827 real sec... [M::process] read 694684 sequences (100000095 bp) in 1.258 CPU sec, 1.273 real sec... Received only 12 licenses out of 31 requested for sentieon:klib [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 290511, 25, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2158) [M::mem_pestat] mean and std.dev: (465.34, 460.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (273.14, 135.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 459, 2082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5648) [M::mem_pestat] mean and std.dev: (922.28, 832.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7431) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 330, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1380) [M::mem_pestat] mean and std.dev: (396.50, 311.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 290273, 37, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 218, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (247.72, 137.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (277.60, 138.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (137, 540, 2279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6563) [M::mem_pestat] mean and std.dev: (847.91, 1144.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8705) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 290, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1785) [M::mem_pestat] mean and std.dev: (378.54, 328.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 289512, 28, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 252, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1234) [M::mem_pestat] mean and std.dev: (349.48, 271.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (278.12, 136.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (547, 931, 1811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4339) [M::mem_pestat] mean and std.dev: (919.17, 674.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5603) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 371, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2061) [M::mem_pestat] mean and std.dev: (553.40, 471.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2674) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696134 reads in 203.835 CPU sec, 16.809 real sec sorting block 3, #reads = 1088311 mem = 536879203 peak = 890007552 [M::mem_process_seqs] Processed 695178 reads in 154.084 CPU sec, 12.862 real sec [M::mem_process_seqs] Processed 694108 reads in 169.196 CPU sec, 14.078 real sec sorting block 4, #reads = 1089061 mem = 536881181 peak = 890007552 [M::process] read 694000 sequences (100000269 bp) in 1.894 CPU sec, 1.970 real sec... [M::process] read 693172 sequences (100000097 bp) in 2.117 CPU sec, 2.317 real sec... [M::process] read 700162 sequences (100000085 bp) in 1.249 CPU sec, 1.255 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 289705, 23, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 279, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (308.15, 191.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 253, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (276.68, 136.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (141, 341, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2493) [M::mem_pestat] mean and std.dev: (550.10, 588.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 381, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1635) [M::mem_pestat] mean and std.dev: (408.06, 259.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2109) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 289413, 33, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 311, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1531) [M::mem_pestat] mean and std.dev: (407.98, 299.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 259, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (281.23, 137.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (112, 570, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3688) [M::mem_pestat] mean and std.dev: (648.37, 683.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4880) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 329, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524) [M::mem_pestat] mean and std.dev: (411.33, 308.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 289477, 26, 94) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 273, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (265.14, 131.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (278.16, 136.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 552, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3039) [M::mem_pestat] mean and std.dev: (743.27, 710.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3981) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 262, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1604) [M::mem_pestat] mean and std.dev: (338.30, 278.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694684 reads in 210.342 CPU sec, 17.353 real sec sorting block 5, #reads = 1085767 mem = 536883279 peak = 890007552 [M::mem_process_seqs] Processed 693172 reads in 152.045 CPU sec, 12.701 real sec [M::mem_process_seqs] Processed 694000 reads in 167.113 CPU sec, 13.927 real sec sorting block 6, #reads = 1086783 mem = 536881100 peak = 890007552 [M::process] read 700438 sequences (100000095 bp) in 2.147 CPU sec, 2.272 real sec... [M::process] read 699704 sequences (100000253 bp) in 2.053 CPU sec, 2.175 real sec... [M::process] read 698986 sequences (100000245 bp) in 1.780 CPU sec, 1.774 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 292191, 23, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 306, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344) [M::mem_pestat] mean and std.dev: (343.86, 260.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (262.97, 132.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 513, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2842) [M::mem_pestat] mean and std.dev: (427.16, 364.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 303, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515) [M::mem_pestat] mean and std.dev: (407.19, 345.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 292410, 24, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 240, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1799) [M::mem_pestat] mean and std.dev: (358.37, 304.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (263.81, 133.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (361, 768, 1764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4570) [M::mem_pestat] mean and std.dev: (901.68, 791.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 323, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1939) [M::mem_pestat] mean and std.dev: (427.16, 343.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 291705, 18, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 319, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3427) [M::mem_pestat] mean and std.dev: (714.72, 860.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (265.04, 133.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (101, 475, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898) [M::mem_pestat] mean and std.dev: (464.94, 372.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 312, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (310.81, 160.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700162 reads in 215.287 CPU sec, 17.736 real sec sorting block 7, #reads = 1086953 mem = 536881071 peak = 890007552 [M::mem_process_seqs] Processed 700438 reads in 153.192 CPU sec, 12.821 real sec [M::mem_process_seqs] Processed 699704 reads in 168.065 CPU sec, 14.055 real sec sorting block 8, #reads = 1091970 mem = 536877842 peak = 890007552 [M::process] read 697328 sequences (100000071 bp) in 2.267 CPU sec, 2.433 real sec... [M::process] read 696630 sequences (100000289 bp) in 2.504 CPU sec, 2.663 real sec... Received only 12 licenses out of 31 requested for sentieon:klib [M::process] read 695948 sequences (100000291 bp) in 1.420 CPU sec, 1.423 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 291806, 31, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 363, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1541) [M::mem_pestat] mean and std.dev: (429.53, 318.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (268.17, 135.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 465, 1345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3609) [M::mem_pestat] mean and std.dev: (714.86, 614.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 304, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (337.73, 198.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 291364, 27, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 286, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (342.32, 250.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (274.52, 139.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (243, 595, 1651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4467) [M::mem_pestat] mean and std.dev: (1120.19, 1131.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 340, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1594) [M::mem_pestat] mean and std.dev: (412.54, 327.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 290530, 29, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 314, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839) [M::mem_pestat] mean and std.dev: (364.06, 267.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 251, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (276.66, 139.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (634, 1117, 2964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7624) [M::mem_pestat] mean and std.dev: (1648.68, 1687.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 347, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2236) [M::mem_pestat] mean and std.dev: (579.05, 457.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698986 reads in 217.634 CPU sec, 17.967 real sec sorting block 9, #reads = 1091294 mem = 536878462 peak = 890007552 [M::mem_process_seqs] Processed 697328 reads in 155.708 CPU sec, 13.057 real sec [M::process] read 694840 sequences (100000044 bp) in 2.369 CPU sec, 2.533 real sec... [M::process] read 694192 sequences (100000257 bp) in 1.480 CPU sec, 1.487 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 290150, 29, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 336, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151) [M::mem_pestat] mean and std.dev: (354.58, 253.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.68, 138.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 526, 1520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3950) [M::mem_pestat] mean and std.dev: (756.54, 587.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5165) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 311, 774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916) [M::mem_pestat] mean and std.dev: (458.00, 378.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695948 reads in 81.774 CPU sec, 6.651 real sec sorting block 10, #reads = 1090129 mem = 536878332 peak = 890007552 [M::mem_process_seqs] Processed 696630 reads in 262.314 CPU sec, 21.751 real sec sorting block 11, #reads = 1088374 mem = 536878009 peak = 925929472 [M::process] read 694672 sequences (100000042 bp) in 2.122 CPU sec, 2.255 real sec... [M::process] read 694504 sequences (100000217 bp) in 1.480 CPU sec, 1.479 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 290301, 30, 96) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 301, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177) [M::mem_pestat] mean and std.dev: (338.51, 206.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (279.00, 138.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 379, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2287) [M::mem_pestat] mean and std.dev: (436.44, 411.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3016) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 308, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (321.38, 168.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1283) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 289962, 28, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 282, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2583) [M::mem_pestat] mean and std.dev: (509.90, 532.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 253, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (276.50, 136.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 820, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3076) [M::mem_pestat] mean and std.dev: (759.69, 674.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4031) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 342, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315) [M::mem_pestat] mean and std.dev: (389.49, 274.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 289860, 34, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 294, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (308.16, 185.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (279.30, 137.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (203, 608, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4340) [M::mem_pestat] mean and std.dev: (813.85, 746.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 293, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1275) [M::mem_pestat] mean and std.dev: (342.48, 245.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1636) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694840 reads in 225.238 CPU sec, 18.592 real sec [M::mem_process_seqs] Processed 694672 reads in 154.142 CPU sec, 12.879 real sec sorting block 12, #reads = 1086975 mem = 536877711 peak = 925929472 [M::mem_process_seqs] Processed 694192 reads in 168.149 CPU sec, 14.023 real sec [M::process] read 694278 sequences (100000097 bp) in 2.444 CPU sec, 2.620 real sec... sorting block 13, #reads = 1087092 mem = 536877999 peak = 925929472 [M::process] read 699526 sequences (100000034 bp) in 1.973 CPU sec, 2.122 real sec... [M::process] read 700758 sequences (100000094 bp) in 1.440 CPU sec, 1.435 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 292183, 33, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 366, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1925) [M::mem_pestat] mean and std.dev: (494.55, 449.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (265.04, 132.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (76, 741, 2164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6340) [M::mem_pestat] mean and std.dev: (1038.00, 1106.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 268, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (323.80, 224.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699526 reads in 76.298 CPU sec, 6.372 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 289885, 28, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 283, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2171) [M::mem_pestat] mean and std.dev: (504.84, 469.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 254, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (276.85, 136.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (170, 429, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2519) [M::mem_pestat] mean and std.dev: (513.04, 521.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 268, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2282) [M::mem_pestat] mean and std.dev: (469.48, 448.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700174 sequences (100000132 bp) in 1.613 CPU sec, 1.614 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 289842, 35, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 323, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1697) [M::mem_pestat] mean and std.dev: (408.67, 311.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 255, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (278.16, 137.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (230, 839, 2156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6008) [M::mem_pestat] mean and std.dev: (927.50, 918.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 250, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (309.07, 181.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694504 reads in 222.931 CPU sec, 18.308 real sec sorting block 14, #reads = 1088424 mem = 536882401 peak = 925929472 Received only 12 licenses out of 31 requested for sentieon:klib [M::mem_process_seqs] Processed 694278 reads in 163.946 CPU sec, 13.580 real sec sorting block 15, #reads = 1086604 mem = 536881060 peak = 925929472 [M::process] read 698746 sequences (100000070 bp) in 2.598 CPU sec, 2.767 real sec... [M::process] read 697870 sequences (100000099 bp) in 1.500 CPU sec, 1.503 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 291975, 18, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1546) [M::mem_pestat] mean and std.dev: (404.10, 296.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 239, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (264.01, 132.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (96, 343, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3273) [M::mem_pestat] mean and std.dev: (410.00, 414.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4332) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 451, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2510) [M::mem_pestat] mean and std.dev: (623.46, 625.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 292390, 22, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 214, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (266.84, 188.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (262.14, 132.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (575, 1894, 4599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12647) [M::mem_pestat] mean and std.dev: (2243.45, 1965.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 334, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1529) [M::mem_pestat] mean and std.dev: (406.52, 312.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700174 reads in 143.956 CPU sec, 11.768 real sec [M::mem_process_seqs] Processed 700758 reads in 157.289 CPU sec, 12.919 real sec sorting block 16, #reads = 1090738 mem = 536880358 peak = 925929472 [M::process] read 696956 sequences (100000091 bp) in 2.183 CPU sec, 2.321 real sec... [M::process] read 696244 sequences (100000182 bp) in 1.450 CPU sec, 1.448 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 291646, 25, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 401, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1909) [M::mem_pestat] mean and std.dev: (484.68, 409.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (272.96, 138.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (474, 670, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2931) [M::mem_pestat] mean and std.dev: (761.38, 470.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 268, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2785) [M::mem_pestat] mean and std.dev: (546.57, 545.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697870 reads in 73.000 CPU sec, 6.100 real sec sorting block 17, #reads = 1090518 mem = 536881600 peak = 925929472 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 291497, 28, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 257, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (260.57, 133.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.37, 135.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (520, 1094, 2039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5077) [M::mem_pestat] mean and std.dev: (1183.15, 818.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 295, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2077) [M::mem_pestat] mean and std.dev: (421.86, 428.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695902 sequences (100000001 bp) in 1.491 CPU sec, 1.492 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 290652, 37, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 281, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210) [M::mem_pestat] mean and std.dev: (476.53, 481.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (275.12, 139.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 432, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2911) [M::mem_pestat] mean and std.dev: (614.76, 635.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3822) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 389, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1135) [M::mem_pestat] mean and std.dev: (352.12, 183.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698746 reads in 216.438 CPU sec, 17.738 real sec [M::mem_process_seqs] Processed 696956 reads in 160.624 CPU sec, 13.321 real sec sorting block 18, #reads = 1089646 mem = 536879015 peak = 925929472 [M::process] read 695848 sequences (100000115 bp) in 2.661 CPU sec, 2.833 real sec... [M::process] read 695696 sequences (100000273 bp) in 1.400 CPU sec, 1.402 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 290777, 23, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 316, 731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1847) [M::mem_pestat] mean and std.dev: (496.89, 491.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (275.06, 138.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (443, 1055, 1547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3755) [M::mem_pestat] mean and std.dev: (1041.50, 719.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 349, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1447) [M::mem_pestat] mean and std.dev: (460.48, 349.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 290513, 23, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 304, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1530) [M::mem_pestat] mean and std.dev: (368.24, 298.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (276.12, 139.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (105, 169, 761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2073) [M::mem_pestat] mean and std.dev: (312.43, 334.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2729) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 218, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1275) [M::mem_pestat] mean and std.dev: (318.89, 272.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1642) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695902 reads in 141.187 CPU sec, 11.578 real sec sorting block 19, #reads = 1087846 mem = 536879409 peak = 925929472 [M::mem_process_seqs] Processed 696244 reads in 155.360 CPU sec, 12.789 real sec [M::process] read 695032 sequences (100000115 bp) in 2.469 CPU sec, 2.635 real sec... [M::process] read 694438 sequences (100000270 bp) in 1.350 CPU sec, 1.344 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 290480, 23, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 330, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (328.74, 179.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (272.90, 135.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 810, 2185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6131) [M::mem_pestat] mean and std.dev: (1034.05, 1001.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8104) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 281, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (264.15, 143.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 290426, 34, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 330, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (331.15, 195.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (273.60, 135.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 578, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2562) [M::mem_pestat] mean and std.dev: (593.03, 527.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 326, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250) [M::mem_pestat] mean and std.dev: (358.93, 254.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695848 reads in 134.565 CPU sec, 11.022 real sec sorting block 20, #reads = 1088186 mem = 536880541 peak = 925929472 [M::mem_process_seqs] Processed 695696 reads in 147.565 CPU sec, 12.129 real sec [M::process] read 699144 sequences (100000197 bp) in 2.199 CPU sec, 2.335 real sec... Received only 12 licenses out of 31 requested for sentieon:klib [M::process] read 700538 sequences (100000283 bp) in 1.290 CPU sec, 1.295 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 290191, 24, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 290, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1906) [M::mem_pestat] mean and std.dev: (439.80, 366.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 254, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (277.78, 137.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 659, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3966) [M::mem_pestat] mean and std.dev: (707.96, 602.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 326, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442) [M::mem_pestat] mean and std.dev: (379.40, 236.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 289742, 29, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 309, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (371.99, 281.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (277.63, 137.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 608, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2612) [M::mem_pestat] mean and std.dev: (690.93, 560.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3378) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1647) [M::mem_pestat] mean and std.dev: (450.90, 434.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695032 reads in 126.586 CPU sec, 10.353 real sec sorting block 21, #reads = 1087277 mem = 536883635 peak = 925929472 [M::mem_process_seqs] Processed 694438 reads in 141.176 CPU sec, 11.592 real sec sorting block 22, #reads = 1086968 mem = 536880853 peak = 996888576 [M::process] read 699094 sequences (100000006 bp) in 2.275 CPU sec, 2.412 real sec... [M::process] read 698190 sequences (100000191 bp) in 1.310 CPU sec, 1.316 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 292425, 21, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 267, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1771) [M::mem_pestat] mean and std.dev: (412.57, 369.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (263.79, 133.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (147, 305, 3711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10839) [M::mem_pestat] mean and std.dev: (2090.81, 3039.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 325, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443) [M::mem_pestat] mean and std.dev: (349.81, 226.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 291054, 25, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 300, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769) [M::mem_pestat] mean and std.dev: (389.89, 317.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (265.85, 133.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 653, 1385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3735) [M::mem_pestat] mean and std.dev: (653.64, 605.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 308, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (304.56, 154.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700538 reads in 125.115 CPU sec, 10.228 real sec [M::mem_process_seqs] Processed 699144 reads in 137.935 CPU sec, 11.327 real sec sorting block 23, #reads = 1089785 mem = 536879749 peak = 996888576 [M::process] read 697184 sequences (100000052 bp) in 2.130 CPU sec, 2.265 real sec... [M::process] read 696768 sequences (100000078 bp) in 1.440 CPU sec, 1.444 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 292054, 20, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 288, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1462) [M::mem_pestat] mean and std.dev: (332.74, 274.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (267.75, 135.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (150, 1050, 1324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3672) [M::mem_pestat] mean and std.dev: (874.05, 791.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 275, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (313.48, 206.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 291501, 30, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 353, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1583) [M::mem_pestat] mean and std.dev: (455.59, 360.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2049) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (270.39, 136.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (363, 649, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746) [M::mem_pestat] mean and std.dev: (561.69, 356.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 311, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (409.35, 310.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699094 reads in 127.990 CPU sec, 10.458 real sec sorting block 24, #reads = 1090743 mem = 536878736 peak = 996888576 [M::mem_process_seqs] Processed 698190 reads in 79.217 CPU sec, 6.646 real sec [M::process] read 696754 sequences (100000120 bp) in 2.269 CPU sec, 2.429 real sec... [M::process] read 697200 sequences (100000117 bp) in 1.350 CPU sec, 1.348 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 290455, 29, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 338, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769) [M::mem_pestat] mean and std.dev: (454.64, 416.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (274.97, 139.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (222, 585, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3411) [M::mem_pestat] mean and std.dev: (693.63, 647.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 363, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328) [M::mem_pestat] mean and std.dev: (350.33, 220.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1703) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 290964, 20, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 332, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (288.44, 144.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (273.58, 138.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 543, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2992) [M::mem_pestat] mean and std.dev: (530.24, 697.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 292, 1334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3620) [M::mem_pestat] mean and std.dev: (578.13, 614.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696768 reads in 131.760 CPU sec, 10.803 real sec sorting block 25, #reads = 1090064 mem = 536879932 peak = 996888576 [M::mem_process_seqs] Processed 697184 reads in 145.099 CPU sec, 11.948 real sec sorting block 26, #reads = 1088452 mem = 536877837 peak = 996888576 [M::process] read 697324 sequences (100000151 bp) in 3.023 CPU sec, 3.218 real sec... [M::process] read 696434 sequences (100000282 bp) in 1.356 CPU sec, 1.363 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 290550, 36, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 311, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1496) [M::mem_pestat] mean and std.dev: (387.41, 306.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (274.29, 139.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (316, 645, 1525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3943) [M::mem_pestat] mean and std.dev: (872.29, 783.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 311, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258) [M::mem_pestat] mean and std.dev: (318.24, 174.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1611) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 290968, 25, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 209, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (260.32, 168.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (271.20, 136.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (504, 1029, 2264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5784) [M::mem_pestat] mean and std.dev: (1268.60, 933.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 322, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2642) [M::mem_pestat] mean and std.dev: (534.81, 516.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696754 reads in 136.544 CPU sec, 11.178 real sec [M::mem_process_seqs] Processed 697200 reads in 148.718 CPU sec, 12.333 real sec sorting block 27, #reads = 1089822 mem = 536878701 peak = 996888576 [M::process] read 695726 sequences (100000278 bp) in 2.109 CPU sec, 2.245 real sec... [M::process] read 695026 sequences (100000211 bp) in 1.320 CPU sec, 1.324 real sec... Received only 12 licenses out of 31 requested for sentieon:klib [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 290337, 34, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 318, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (355.44, 240.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 246, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (270.29, 134.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (401, 689, 1189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2765) [M::mem_pestat] mean and std.dev: (655.17, 413.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 314, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1837) [M::mem_pestat] mean and std.dev: (419.51, 336.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696434 reads in 69.484 CPU sec, 5.799 real sec sorting block 28, #reads = 1088421 mem = 536878053 peak = 996888576 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 290512, 36, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 261, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1996) [M::mem_pestat] mean and std.dev: (413.95, 370.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (270.55, 136.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (278, 563, 1669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4451) [M::mem_pestat] mean and std.dev: (809.36, 821.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5842) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 294, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (343.80, 224.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700358 sequences (100000208 bp) in 1.493 CPU sec, 1.501 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 290261, 28, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 260, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (269.39, 145.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (273.06, 135.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (526, 842, 1723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4117) [M::mem_pestat] mean and std.dev: (842.60, 625.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5314) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 279, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1474) [M::mem_pestat] mean and std.dev: (320.79, 257.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697324 reads in 199.512 CPU sec, 16.441 real sec [M::mem_process_seqs] Processed 695726 reads in 150.377 CPU sec, 12.463 real sec sorting block 29, #reads = 1088530 mem = 536876301 peak = 996888576 [M::process] read 704634 sequences (100000140 bp) in 2.576 CPU sec, 2.751 real sec... [M::process] read 702728 sequences (100000137 bp) in 1.390 CPU sec, 1.388 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (96, 289969, 27, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (369.21, 358.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 251, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (275.22, 136.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (477, 774, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4425) [M::mem_pestat] mean and std.dev: (976.00, 970.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 416, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330) [M::mem_pestat] mean and std.dev: (438.74, 264.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1695) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 291654, 29, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 315, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2004) [M::mem_pestat] mean and std.dev: (440.48, 392.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (262.43, 133.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 689, 2411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6835) [M::mem_pestat] mean and std.dev: (1179.00, 1274.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (294.92, 167.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695026 reads in 131.774 CPU sec, 10.774 real sec [M::mem_process_seqs] Processed 700358 reads in 145.268 CPU sec, 11.926 real sec sorting block 30, #reads = 1086947 mem = 536878180 peak = 996888576 [M::process] read 702574 sequences (100000025 bp) in 2.194 CPU sec, 2.367 real sec... [M::process] read 700790 sequences (100000299 bp) in 1.480 CPU sec, 1.483 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 293529, 17, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (279.02, 192.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 229, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (256.98, 131.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (412, 669, 2487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6637) [M::mem_pestat] mean and std.dev: (1117.06, 1111.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 338, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099) [M::mem_pestat] mean and std.dev: (331.48, 218.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 293956, 19, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 251, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1880) [M::mem_pestat] mean and std.dev: (409.29, 344.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 222, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (249.91, 127.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 372, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1799) [M::mem_pestat] mean and std.dev: (367.28, 319.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 280, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185) [M::mem_pestat] mean and std.dev: (324.22, 212.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702728 reads in 146.784 CPU sec, 12.020 real sec sorting block 31, #reads = 1089344 mem = 536877179 peak = 996888576 [M::mem_process_seqs] Processed 704634 reads in 160.057 CPU sec, 13.148 real sec [M::process] read 700056 sequences (100000188 bp) in 2.406 CPU sec, 2.557 real sec... [M::process] read 699936 sequences (100000210 bp) in 1.519 CPU sec, 1.522 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 293075, 15, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 309, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1138) [M::mem_pestat] mean and std.dev: (353.24, 256.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 228, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (255.04, 129.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 392, 3000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8708) [M::mem_pestat] mean and std.dev: (1302.53, 1411.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 281, 737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861) [M::mem_pestat] mean and std.dev: (428.95, 379.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 292404, 38, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 227, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (216.57, 87.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (263.84, 135.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 474, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2409) [M::mem_pestat] mean and std.dev: (479.03, 530.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 319, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (329.57, 127.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702574 reads in 136.977 CPU sec, 11.181 real sec sorting block 32, #reads = 1091579 mem = 536878539 peak = 996888576 [M::mem_process_seqs] Processed 700790 reads in 149.364 CPU sec, 12.240 real sec sorting block 33, #reads = 1091258 mem = 536879145 peak = 1043197952 [M::process] read 698492 sequences (100000202 bp) in 2.155 CPU sec, 2.317 real sec... [M::process] read 696806 sequences (100000086 bp) in 1.440 CPU sec, 1.439 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 291892, 28, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 229, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155) [M::mem_pestat] mean and std.dev: (288.33, 191.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (266.12, 136.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (216, 500, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120) [M::mem_pestat] mean and std.dev: (666.25, 737.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 294, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1503) [M::mem_pestat] mean and std.dev: (411.59, 316.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR Received only 12 licenses out of 31 requested for sentieon:klib [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 292065, 24, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 480, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2100) [M::mem_pestat] mean and std.dev: (471.38, 357.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 236, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (263.83, 134.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (451, 918, 2288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5962) [M::mem_pestat] mean and std.dev: (1149.43, 1038.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 327, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (323.68, 208.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 291922, 22, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 231, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (310.59, 224.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (269.76, 137.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 588, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3894) [M::mem_pestat] mean and std.dev: (653.05, 563.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5136) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 476, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1686) [M::mem_pestat] mean and std.dev: (447.87, 299.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700056 reads in 195.316 CPU sec, 16.087 real sec [M::mem_process_seqs] Processed 699936 reads in 143.331 CPU sec, 11.986 real sec sorting block 34, #reads = 1088940 mem = 536880616 peak = 1043197952 [M::mem_process_seqs] Processed 698492 reads in 156.579 CPU sec, 13.074 real sec [M::process] read 695414 sequences (100000131 bp) in 2.392 CPU sec, 2.569 real sec... sorting block 35, #reads = 1088362 mem = 536881467 peak = 1043197952 [M::process] read 695090 sequences (100000190 bp) in 1.958 CPU sec, 2.093 real sec... [M::process] read 701826 sequences (100000183 bp) in 1.390 CPU sec, 1.389 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 291380, 38, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 294, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1747) [M::mem_pestat] mean and std.dev: (400.23, 323.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (273.69, 137.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (206, 616, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2390) [M::mem_pestat] mean and std.dev: (741.86, 725.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 348, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1782) [M::mem_pestat] mean and std.dev: (472.79, 380.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2311) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 289924, 29, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 278, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (296.84, 169.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (277.07, 138.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (399, 737, 2688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7266) [M::mem_pestat] mean and std.dev: (1148.88, 1114.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9555) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 328, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1988) [M::mem_pestat] mean and std.dev: (451.91, 375.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696806 reads in 140.564 CPU sec, 11.514 real sec [M::process] read 707686 sequences (100000080 bp) in 1.532 CPU sec, 1.541 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 290466, 21, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 312, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549) [M::mem_pestat] mean and std.dev: (355.55, 238.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (278.16, 139.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 450, 1147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2989) [M::mem_pestat] mean and std.dev: (630.95, 571.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 259, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301) [M::mem_pestat] mean and std.dev: (322.20, 218.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695414 reads in 146.685 CPU sec, 12.122 real sec sorting block 36, #reads = 1084144 mem = 536879749 peak = 1043197952 [M::mem_process_seqs] Processed 695090 reads in 160.416 CPU sec, 13.299 real sec sorting block 37, #reads = 1083924 mem = 536880623 peak = 1043197952 [M::process] read 704572 sequences (100000200 bp) in 2.233 CPU sec, 2.371 real sec... [M::process] read 701994 sequences (100000153 bp) in 1.400 CPU sec, 1.403 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 292469, 18, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 310, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (413.42, 336.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (259.16, 132.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (612, 1089, 1444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3108) [M::mem_pestat] mean and std.dev: (1125.28, 807.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 303, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (293.06, 171.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 295287, 29, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 208, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2224) [M::mem_pestat] mean and std.dev: (441.16, 421.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 216, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (241.57, 120.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (366, 617, 2151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5721) [M::mem_pestat] mean and std.dev: (1201.41, 1371.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 290, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035) [M::mem_pestat] mean and std.dev: (295.47, 174.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 293569, 30, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 313, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2223) [M::mem_pestat] mean and std.dev: (377.12, 314.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 224, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (250.64, 126.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (152, 387, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4310) [M::mem_pestat] mean and std.dev: (639.96, 848.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5696) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 284, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1676) [M::mem_pestat] mean and std.dev: (371.63, 276.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701826 reads in 209.159 CPU sec, 17.220 real sec [M::mem_process_seqs] Processed 707686 reads in 154.268 CPU sec, 12.878 real sec sorting block 38, #reads = 1086580 mem = 536878769 peak = 1043197952 [M::mem_process_seqs] Processed 704572 reads in 169.319 CPU sec, 14.091 real sec [M::process] read 701104 sequences (100000062 bp) in 2.123 CPU sec, 2.283 real sec... sorting block 39, #reads = 1085591 mem = 536877833 peak = 1043197952 [M::process] read 700306 sequences (100000139 bp) in 2.102 CPU sec, 2.248 real sec... [M::process] read 699204 sequences (100000003 bp) in 1.350 CPU sec, 1.346 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 292855, 26, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 343, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016) [M::mem_pestat] mean and std.dev: (461.64, 430.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 233, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (261.24, 133.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (315, 692, 1065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2565) [M::mem_pestat] mean and std.dev: (659.17, 510.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 383, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4490) [M::mem_pestat] mean and std.dev: (737.86, 689.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701104 reads in 74.086 CPU sec, 6.307 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 292921, 25, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 293, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (294.73, 159.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 230, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (257.36, 130.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 350, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2662) [M::mem_pestat] mean and std.dev: (525.50, 584.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 313, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1708) [M::mem_pestat] mean and std.dev: (427.82, 342.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR Received only 12 licenses out of 31 requested for sentieon:klib [M::process] read 698590 sequences (100000012 bp) in 1.493 CPU sec, 1.494 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 292207, 19, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 277, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1234) [M::mem_pestat] mean and std.dev: (338.10, 252.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (263.43, 133.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (463, 1425, 1707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4195) [M::mem_pestat] mean and std.dev: (864.40, 583.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5439) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 281, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1652) [M::mem_pestat] mean and std.dev: (411.34, 322.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701994 reads in 217.780 CPU sec, 17.969 real sec sorting block 40, #reads = 1086119 mem = 536879460 peak = 1043197952 [M::mem_process_seqs] Processed 700306 reads in 162.591 CPU sec, 13.593 real sec sorting block 41, #reads = 1086157 mem = 536880198 peak = 1043197952 [M::process] read 696530 sequences (100000231 bp) in 2.404 CPU sec, 2.565 real sec... [M::process] read 696324 sequences (100000144 bp) in 1.350 CPU sec, 1.352 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 291872, 21, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 254, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (269.51, 134.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (267.08, 135.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 743, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3340) [M::mem_pestat] mean and std.dev: (784.55, 632.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 342, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2322) [M::mem_pestat] mean and std.dev: (541.67, 489.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 291786, 18, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 264, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1963) [M::mem_pestat] mean and std.dev: (352.05, 358.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 241, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (268.89, 136.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 609, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2674) [M::mem_pestat] mean and std.dev: (749.11, 664.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 320, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (348.10, 227.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699204 reads in 136.013 CPU sec, 11.128 real sec [M::mem_process_seqs] Processed 698590 reads in 149.489 CPU sec, 12.265 real sec sorting block 42, #reads = 1085932 mem = 536882545 peak = 1043197952 [M::process] read 696996 sequences (100000172 bp) in 2.419 CPU sec, 2.560 real sec... [M::process] read 705762 sequences (100000196 bp) in 1.370 CPU sec, 1.370 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 290333, 27, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 309, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2278) [M::mem_pestat] mean and std.dev: (411.83, 345.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 247, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (275.31, 140.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (76, 421, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (252.45, 218.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2056) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 316, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1793) [M::mem_pestat] mean and std.dev: (486.73, 437.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2319) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 291273, 16, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 334, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1589) [M::mem_pestat] mean and std.dev: (451.88, 365.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.06, 140.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (715, 1053, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2398) [M::mem_pestat] mean and std.dev: (1021.81, 527.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 294, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115) [M::mem_pestat] mean and std.dev: (319.22, 209.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696324 reads in 135.877 CPU sec, 11.153 real sec [M::mem_process_seqs] Processed 696530 reads in 147.436 CPU sec, 12.184 real sec sorting block 43, #reads = 1086409 mem = 536877851 peak = 1043197952 [M::process] read 702844 sequences (100000248 bp) in 2.180 CPU sec, 2.340 real sec... [M::process] read 701490 sequences (100000234 bp) in 1.399 CPU sec, 1.395 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 294171, 24, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 374, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1715) [M::mem_pestat] mean and std.dev: (486.03, 394.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 221, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (247.26, 125.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (271, 800, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3820) [M::mem_pestat] mean and std.dev: (818.00, 629.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 287, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1188) [M::mem_pestat] mean and std.dev: (326.56, 228.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 291091, 18, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 321, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (358.33, 230.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 244, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (271.86, 138.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 592, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2347) [M::mem_pestat] mean and std.dev: (632.35, 628.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3147) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 450, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1649) [M::mem_pestat] mean and std.dev: (398.57, 282.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2125) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705762 reads in 139.020 CPU sec, 11.369 real sec sorting block 44, #reads = 1087730 mem = 536879033 peak = 1043197952 [M::process] read 700400 sequences (100000025 bp) in 1.440 CPU sec, 1.452 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 293141, 17, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 289, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1173) [M::mem_pestat] mean and std.dev: (314.80, 223.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 228, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (254.91, 128.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 380, 1822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5222) [M::mem_pestat] mean and std.dev: (755.00, 980.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 362, 1232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3342) [M::mem_pestat] mean and std.dev: (708.84, 739.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702844 reads in 71.991 CPU sec, 5.903 real sec [M::mem_process_seqs] Processed 696996 reads in 236.040 CPU sec, 19.434 real sec sorting block 45, #reads = 1090414 mem = 536878225 peak = 1043197952 [M::process] read 699384 sequences (100000112 bp) in 2.216 CPU sec, 2.395 real sec... [M::process] read 699064 sequences (100000115 bp) in 1.350 CPU sec, 1.343 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 292917, 17, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 221, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417) [M::mem_pestat] mean and std.dev: (330.66, 280.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 233, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (260.37, 132.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (229, 520, 1867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5143) [M::mem_pestat] mean and std.dev: (1204.76, 1436.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 346, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1556) [M::mem_pestat] mean and std.dev: (403.26, 289.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 292622, 16, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 283, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (299.58, 179.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (265.14, 135.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 610, 1805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5161) [M::mem_pestat] mean and std.dev: (1072.13, 1484.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 313, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2048) [M::mem_pestat] mean and std.dev: (529.72, 469.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR Received only 12 licenses out of 31 requested for sentieon:klib [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 292484, 27, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 333, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505) [M::mem_pestat] mean and std.dev: (610.66, 561.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 239, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (267.39, 136.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 528, 1501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4331) [M::mem_pestat] mean and std.dev: (812.16, 1068.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 310, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (360.98, 257.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701490 reads in 255.734 CPU sec, 21.018 real sec sorting block 46, #reads = 1085022 mem = 536878968 peak = 1043197952 [M::mem_process_seqs] Processed 700400 reads in 135.291 CPU sec, 11.309 real sec [M::mem_process_seqs] Processed 699384 reads in 148.680 CPU sec, 12.401 real sec sorting block 47, #reads = 1089937 mem = 536883332 peak = 1043197952 [M::process] read 698576 sequences (100000292 bp) in 2.463 CPU sec, 2.646 real sec... [M::process] read 697562 sequences (100000006 bp) in 1.984 CPU sec, 2.101 real sec... [M::process] read 697224 sequences (100000108 bp) in 1.370 CPU sec, 1.370 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 292029, 27, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 321, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507) [M::mem_pestat] mean and std.dev: (437.85, 365.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (269.35, 138.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (197, 470, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2546) [M::mem_pestat] mean and std.dev: (574.33, 585.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 329, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (340.86, 185.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 291845, 20, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 324, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2333) [M::mem_pestat] mean and std.dev: (434.45, 399.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (269.75, 137.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (208, 604, 1294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3466) [M::mem_pestat] mean and std.dev: (607.32, 527.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 296, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (330.72, 200.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 291793, 28, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 270, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441) [M::mem_pestat] mean and std.dev: (366.20, 298.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (271.13, 137.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (291, 789, 2075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5643) [M::mem_pestat] mean and std.dev: (1131.60, 1391.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7427) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 286, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1654) [M::mem_pestat] mean and std.dev: (328.49, 284.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699064 reads in 191.837 CPU sec, 15.787 real sec sorting block 48, #reads = 1087839 mem = 536877881 peak = 1043197952 [M::mem_process_seqs] Processed 698576 reads in 139.931 CPU sec, 11.685 real sec [M::mem_process_seqs] Processed 697562 reads in 153.796 CPU sec, 12.811 real sec [M::process] read 697424 sequences (100000060 bp) in 2.449 CPU sec, 2.616 real sec... sorting block 49, #reads = 1087043 mem = 536878943 peak = 1043197952 [M::process] read 697246 sequences (100000108 bp) in 1.937 CPU sec, 2.042 real sec... [M::process] read 706856 sequences (100000087 bp) in 1.399 CPU sec, 1.395 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 291222, 26, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 278, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1686) [M::mem_pestat] mean and std.dev: (391.54, 327.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (271.16, 136.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (417, 756, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2817) [M::mem_pestat] mean and std.dev: (744.83, 529.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 395, 1430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3762) [M::mem_pestat] mean and std.dev: (859.77, 924.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 290845, 30, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 378, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1740) [M::mem_pestat] mean and std.dev: (442.25, 361.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (271.50, 137.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 681, 1957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5513) [M::mem_pestat] mean and std.dev: (1036.80, 1098.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7291) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 311, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163) [M::mem_pestat] mean and std.dev: (361.52, 264.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 290917, 30, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 248, 799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2059) [M::mem_pestat] mean and std.dev: (506.04, 516.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (270.35, 136.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (236, 646, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2999) [M::mem_pestat] mean and std.dev: (636.61, 542.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3920) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 262, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (331.32, 213.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1600) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697224 reads in 200.377 CPU sec, 16.503 real sec sorting block 50, #reads = 1085962 mem = 536877227 peak = 1043197952 [M::mem_process_seqs] Processed 697246 reads in 147.249 CPU sec, 12.285 real sec [M::mem_process_seqs] Processed 697424 reads in 161.680 CPU sec, 13.450 real sec [M::process] read 707944 sequences (100000187 bp) in 2.367 CPU sec, 2.524 real sec... sorting block 51, #reads = 1082851 mem = 536878601 peak = 1043197952 [M::process] read 704162 sequences (100000222 bp) in 1.943 CPU sec, 2.038 real sec... [M::process] read 701998 sequences (100000184 bp) in 1.330 CPU sec, 1.336 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 294399, 43, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 229, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1310) [M::mem_pestat] mean and std.dev: (261.77, 190.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 217, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (243.22, 122.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (347, 599, 1534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3908) [M::mem_pestat] mean and std.dev: (819.98, 754.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5095) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 299, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2279) [M::mem_pestat] mean and std.dev: (464.57, 484.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 293779, 16, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 228, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (292.70, 227.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 225, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (251.62, 126.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 373, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862) [M::mem_pestat] mean and std.dev: (433.29, 371.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 232, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (233.70, 124.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 295069, 26, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 323, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2586) [M::mem_pestat] mean and std.dev: (484.59, 402.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 215, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (241.51, 121.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (387, 645, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2097) [M::mem_pestat] mean and std.dev: (694.54, 475.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2667) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 408, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2874) [M::mem_pestat] mean and std.dev: (557.75, 545.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR Received only 12 licenses out of 31 requested for sentieon:klib [M::mem_process_seqs] Processed 706856 reads in 213.287 CPU sec, 17.568 real sec sorting block 52, #reads = 1084067 mem = 536883640 peak = 1043197952 [M::mem_process_seqs] Processed 704162 reads in 153.857 CPU sec, 12.832 real sec [M::mem_process_seqs] Processed 707944 reads in 169.737 CPU sec, 14.117 real sec [M::process] read 700632 sequences (100000104 bp) in 2.527 CPU sec, 2.670 real sec... sorting block 53, #reads = 1086190 mem = 536878677 peak = 1043197952 [M::process] read 699992 sequences (100000016 bp) in 2.040 CPU sec, 2.148 real sec... [M::process] read 698810 sequences (100000201 bp) in 1.360 CPU sec, 1.366 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 293067, 13, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 214, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (228.73, 104.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (259.06, 131.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 302, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (327.92, 192.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 344, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (366.40, 218.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 291960, 26, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 397, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2317) [M::mem_pestat] mean and std.dev: (640.39, 611.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 237, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (264.84, 135.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (108, 683, 1683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4833) [M::mem_pestat] mean and std.dev: (1070.00, 1227.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6408) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 275, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861) [M::mem_pestat] mean and std.dev: (400.18, 376.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701998 reads in 141.998 CPU sec, 11.633 real sec sorting block 54, #reads = 1086469 mem = 536885506 peak = 1043197952 [M::process] read 698880 sequences (100000034 bp) in 1.468 CPU sec, 1.471 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 292320, 17, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 341, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1532) [M::mem_pestat] mean and std.dev: (374.25, 240.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (263.33, 133.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 574, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4871) [M::mem_pestat] mean and std.dev: (842.12, 876.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 278, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (280.02, 137.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699992 reads in 149.192 CPU sec, 12.336 real sec [M::process] read 697760 sequences (100000043 bp) in 1.464 CPU sec, 1.477 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 291415, 23, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 226, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (260.71, 167.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 239, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (267.05, 135.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (411, 556, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2751) [M::mem_pestat] mean and std.dev: (597.80, 517.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 477, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2003) [M::mem_pestat] mean and std.dev: (615.55, 491.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698880 reads in 75.619 CPU sec, 6.223 real sec sorting block 55, #reads = 1086007 mem = 536876629 peak = 1043197952 [M::mem_process_seqs] Processed 700632 reads in 247.754 CPU sec, 20.505 real sec [M::process] read 697268 sequences (100000027 bp) in 2.193 CPU sec, 2.328 real sec... [M::process] read 583198 sequences (83670340 bp) in 1.198 CPU sec, 1.206 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 292044, 26, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 228, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (289.08, 208.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 239, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (266.37, 134.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (106, 365, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2839) [M::mem_pestat] mean and std.dev: (566.08, 507.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 406, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1857) [M::mem_pestat] mean and std.dev: (453.05, 366.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 290500, 23, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 431, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3022) [M::mem_pestat] mean and std.dev: (580.69, 453.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 244, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (271.69, 138.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (418, 712, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3607) [M::mem_pestat] mean and std.dev: (1011.95, 801.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 306, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1673) [M::mem_pestat] mean and std.dev: (433.38, 387.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 290965, 25, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 334, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1784) [M::mem_pestat] mean and std.dev: (513.93, 478.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 242, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (268.75, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (353, 586, 5025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14369) [M::mem_pestat] mean and std.dev: (2124.48, 2704.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 364, 1632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4544) [M::mem_pestat] mean and std.dev: (810.60, 867.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6000) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698810 reads in 268.134 CPU sec, 22.079 real sec sorting block 56, #reads = 1086074 mem = 536880154 peak = 1043197952 [M::mem_process_seqs] Processed 697268 reads in 140.363 CPU sec, 11.745 real sec sorting block 57, #reads = 1086721 mem = 536880175 peak = 1134096384 [M::mem_process_seqs] Processed 697760 reads in 153.956 CPU sec, 13.094 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 243294, 25, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 271, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2138) [M::mem_pestat] mean and std.dev: (400.66, 370.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 245, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (272.05, 138.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (242, 503, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3731) [M::mem_pestat] mean and std.dev: (868.16, 911.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 306, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1996) [M::mem_pestat] mean and std.dev: (453.91, 383.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 583198 reads in 61.894 CPU sec, 5.556 real sec sorting block 58, #reads = 1086020 mem = 536881304 peak = 1174269952 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=12 reads: 64156576 overall: 69883523072 mem 8221.728 user 342.856 sys 1320.218 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_27F-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:1136_27F-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_27F-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1136_27F-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 1320.254 sec; CPU: 8564.586 sec sorting block 59, #reads = 511379 mem = 252894080 peak = 1174269952 uniq header count 1 merging 60 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 3629444602 output reads: 64697775 bam_mem_sort: 60 calls 24.117 user 0.498 sys 24.776 real bam_write: 60 calls 42.601 user 1.147 sys 43.919 real execute: 1 calls 11.612 user 0.766 sys 1339.581 real merge_files: 1 calls 11.593 user 0.747 sys 18.577 real parse_chunk: 952 calls 31.360 user 8.771 sys 41.116 real read_chunk: 2573 calls 24.521 user 23.060 sys 572.472 real sort_block: 1 calls 19.273 user 22.524 sys 564.609 real write_chunk: 1619 calls 11.312 user 0.538 sys 17.869 real overall: 2738749440 mem 718.119 user 41.228 sys 1339.603 real ls: cannot access '*.cram': No such file or directory