.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_cfy-T1-TDNA-1_A23MHWWLT4_1;L004;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/04/6882882d65fd12c273560464681783/.command.err
Size: 209.0 KB
Task: DAQ:SENTIEON_BWAMEM (659_cfy-T1-TDNA-1_A23MHWWLT4_1;L004;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cfy-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1475.169 CPU sec, 1008.418 real sec
[M::process] read 688708 sequences (100000134 bp) in 0.937 CPU sec, 0.939 real sec...
[M::process] read 688450 sequences (100000150 bp) in 1.214 CPU sec, 1.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287421, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 264, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (286.12, 135.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688518 sequences (100000288 bp) in 1.192 CPU sec, 1.204 real sec...
[M::process] read 688012 sequences (100000268 bp) in 1.170 CPU sec, 1.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287032, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (288.66, 137.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688708 reads in 137.595 CPU sec, 4.353 real sec
[M::mem_process_seqs] Processed 688450 reads in 76.565 CPU sec, 2.439 real sec
sorting block 0, #reads = 1088833 mem = 536876920 peak = 608546816
[M::process] read 687700 sequences (100000233 bp) in 1.449 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287673, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (288.80, 137.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687204 sequences (100000093 bp) in 1.180 CPU sec, 1.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286926, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (289.09, 136.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 962)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287179, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (292.00, 138.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688518 reads in 176.406 CPU sec, 5.645 real sec
[M::mem_process_seqs] Processed 688012 reads in 131.255 CPU sec, 4.221 real sec
[M::mem_process_seqs] Processed 687700 reads in 142.389 CPU sec, 4.596 real sec
sorting block 1, #reads = 1089066 mem = 536883658 peak = 715812864
[M::process] read 686846 sequences (100000202 bp) in 1.612 CPU sec, 1.705 real sec...
sorting block 2, #reads = 1088682 mem = 536879704 peak = 920088576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286593, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (293.53, 139.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686740 sequences (100000160 bp) in 1.366 CPU sec, 1.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286773, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (294.87, 140.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687204 reads in 123.152 CPU sec, 3.908 real sec
[M::mem_process_seqs] Processed 686846 reads in 77.255 CPU sec, 2.431 real sec
[M::process] read 685934 sequences (100000188 bp) in 1.430 CPU sec, 1.472 real sec...
sorting block 3, #reads = 1087560 mem = 536878367 peak = 920088576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286387, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (296.45, 140.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686076 sequences (100000292 bp) in 1.594 CPU sec, 1.685 real sec...
[M::process] read 685682 sequences (100000223 bp) in 1.250 CPU sec, 1.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286516, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.63, 142.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686740 reads in 122.624 CPU sec, 3.903 real sec
[M::mem_process_seqs] Processed 685934 reads in 77.227 CPU sec, 2.453 real sec
sorting block 4, #reads = 1088508 mem = 536882300 peak = 920088576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286278, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.56, 143.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685536 sequences (100000113 bp) in 1.630 CPU sec, 1.720 real sec...
[M::process] read 685364 sequences (100000137 bp) in 1.280 CPU sec, 1.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286161, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (301.64, 143.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686076 reads in 122.432 CPU sec, 3.902 real sec
[M::mem_process_seqs] Processed 685682 reads in 76.284 CPU sec, 2.421 real sec
sorting block 5, #reads = 1088112 mem = 536879495 peak = 920088576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286384, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (302.66, 144.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685172 sequences (100000013 bp) in 1.901 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286404, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (301.81, 143.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685478 sequences (100000045 bp) in 1.320 CPU sec, 1.317 real sec...
[M::mem_process_seqs] Processed 685536 reads in 122.598 CPU sec, 3.898 real sec
sorting block 6, #reads = 1087751 mem = 536882112 peak = 920088576
[M::mem_process_seqs] Processed 685364 reads in 76.732 CPU sec, 2.435 real sec
sorting block 7, #reads = 1087750 mem = 536880962 peak = 920088576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285913, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.45, 143.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685192 sequences (100000076 bp) in 1.715 CPU sec, 1.817 real sec...
[M::process] read 685148 sequences (100000220 bp) in 1.330 CPU sec, 1.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285656, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (299.93, 141.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685172 reads in 125.248 CPU sec, 3.991 real sec
[M::mem_process_seqs] Processed 685478 reads in 78.804 CPU sec, 2.499 real sec
sorting block 8, #reads = 1088100 mem = 536879894 peak = 920088576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285924, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (300.11, 142.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685210 sequences (100000189 bp) in 1.964 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286188, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (300.01, 141.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685596 sequences (100000135 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 685192 reads in 125.303 CPU sec, 3.995 real sec
[M::mem_process_seqs] Processed 685148 reads in 77.943 CPU sec, 2.478 real sec
sorting block 9, #reads = 1086789 mem = 536880393 peak = 920088576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285564, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (299.02, 141.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685656 sequences (100000125 bp) in 1.834 CPU sec, 1.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286050, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (298.44, 141.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685418 sequences (100000021 bp) in 1.350 CPU sec, 1.359 real sec...
[M::mem_process_seqs] Processed 685210 reads in 125.208 CPU sec, 3.991 real sec
sorting block 10, #reads = 1087387 mem = 536878824 peak = 920088576
[M::mem_process_seqs] Processed 685596 reads in 78.601 CPU sec, 2.491 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286469, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (298.95, 142.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 11, #reads = 1087124 mem = 536879178 peak = 920088576
[M::process] read 685214 sequences (100000213 bp) in 2.070 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285785, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (300.09, 142.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684960 sequences (100000108 bp) in 1.370 CPU sec, 1.364 real sec...
[M::mem_process_seqs] Processed 685656 reads in 126.197 CPU sec, 4.015 real sec
[M::mem_process_seqs] Processed 685418 reads in 79.453 CPU sec, 2.516 real sec
sorting block 12, #reads = 1087472 mem = 536881034 peak = 920088576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285390, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (299.90, 142.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684580 sequences (100000299 bp) in 1.849 CPU sec, 1.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285606, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (301.37, 142.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684884 sequences (100000112 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_process_seqs] Processed 685214 reads in 125.809 CPU sec, 4.010 real sec
[M::mem_process_seqs] Processed 684960 reads in 78.396 CPU sec, 2.482 real sec
sorting block 13, #reads = 1087867 mem = 536881167 peak = 920088576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285774, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.43, 142.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684402 sequences (100000091 bp) in 2.135 CPU sec, 2.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286220, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (301.24, 141.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684180 sequences (100000113 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_process_seqs] Processed 684580 reads in 126.286 CPU sec, 4.017 real sec
[M::mem_process_seqs] Processed 684884 reads in 79.150 CPU sec, 2.508 real sec
sorting block 14, #reads = 1086678 mem = 536878924 peak = 920088576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285946, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (302.95, 142.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684428 sequences (100000261 bp) in 1.879 CPU sec, 1.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285336, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (303.91, 142.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683658 sequences (100000028 bp) in 1.380 CPU sec, 1.380 real sec...
[M::mem_process_seqs] Processed 684402 reads in 127.140 CPU sec, 4.055 real sec
sorting block 15, #reads = 1086886 mem = 536882101 peak = 920088576
[M::mem_process_seqs] Processed 684180 reads in 79.100 CPU sec, 2.505 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285600, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (303.71, 142.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1086696 mem = 536879607 peak = 936902656
[M::process] read 683624 sequences (100000115 bp) in 2.172 CPU sec, 2.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285902, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (305.45, 142.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683700 sequences (100000076 bp) in 1.360 CPU sec, 1.356 real sec...
[M::mem_process_seqs] Processed 684428 reads in 125.453 CPU sec, 3.987 real sec
[M::mem_process_seqs] Processed 683658 reads in 77.734 CPU sec, 2.459 real sec
sorting block 17, #reads = 1085586 mem = 536883276 peak = 936902656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285835, 5, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (271, 437, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (373.07, 95.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 287, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (306.56, 143.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 683254 sequences (100000168 bp) in 1.975 CPU sec, 2.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286313, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 288, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (307.98, 144.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683624 reads in 120.220 CPU sec, 3.831 real sec
[M::mem_process_seqs] Processed 683700 reads in 76.022 CPU sec, 2.399 real sec
[M::process] read 683004 sequences (100000079 bp) in 1.515 CPU sec, 1.542 real sec...
sorting block 18, #reads = 1085508 mem = 536877405 peak = 936902656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285659, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (309.07, 145.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685142 sequences (100000250 bp) in 2.102 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284965, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 291, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (310.28, 144.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683254 reads in 120.086 CPU sec, 3.824 real sec
[M::mem_process_seqs] Processed 683004 reads in 76.514 CPU sec, 2.414 real sec
[M::process] read 689054 sequences (100000094 bp) in 1.516 CPU sec, 1.554 real sec...
sorting block 19, #reads = 1087588 mem = 536878475 peak = 936902656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286179, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (302.03, 141.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688520 sequences (100000159 bp) in 1.865 CPU sec, 1.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286921, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (289.77, 139.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685142 reads in 121.830 CPU sec, 3.886 real sec
sorting block 20, #reads = 1086119 mem = 536880121 peak = 936902656
[M::mem_process_seqs] Processed 689054 reads in 77.079 CPU sec, 2.426 real sec
[M::process] read 688340 sequences (100000188 bp) in 1.535 CPU sec, 1.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286631, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (292.17, 140.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1088549 mem = 536877270 peak = 965869568
[M::process] read 688024 sequences (100000119 bp) in 2.140 CPU sec, 2.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286844, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 270, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (292.58, 140.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688520 reads in 120.376 CPU sec, 3.827 real sec
[M::mem_process_seqs] Processed 688340 reads in 76.263 CPU sec, 2.402 real sec
[M::process] read 687742 sequences (100000091 bp) in 1.537 CPU sec, 1.568 real sec...
sorting block 22, #reads = 1089813 mem = 536878542 peak = 965869568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286846, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (293.74, 140.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687448 sequences (100000062 bp) in 1.821 CPU sec, 1.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287583, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 272, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (294.53, 140.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688024 reads in 118.972 CPU sec, 3.788 real sec
[M::mem_process_seqs] Processed 687742 reads in 75.855 CPU sec, 2.386 real sec
[M::process] read 687030 sequences (100000171 bp) in 1.500 CPU sec, 1.525 real sec...
sorting block 23, #reads = 1090028 mem = 536878814 peak = 965869568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287601, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (295.10, 141.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686702 sequences (100000062 bp) in 2.111 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286592, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (297.58, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687448 reads in 119.281 CPU sec, 3.793 real sec
[M::mem_process_seqs] Processed 687030 reads in 75.592 CPU sec, 2.378 real sec
[M::process] read 686214 sequences (100000295 bp) in 1.539 CPU sec, 1.570 real sec...
sorting block 24, #reads = 1090861 mem = 536878483 peak = 965869568
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286675, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (298.43, 142.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686456 sequences (100000150 bp) in 1.865 CPU sec, 2.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286027, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (300.13, 143.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686702 reads in 119.033 CPU sec, 3.794 real sec
sorting block 25, #reads = 1089057 mem = 536880268 peak = 965869568
[M::mem_process_seqs] Processed 686214 reads in 75.791 CPU sec, 2.390 real sec
[M::process] read 685766 sequences (100000146 bp) in 1.582 CPU sec, 1.626 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286463, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (301.82, 144.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1088116 mem = 536881950 peak = 1007165440
[M::process] read 685924 sequences (100000236 bp) in 2.093 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286262, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 282, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (304.28, 145.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686456 reads in 119.204 CPU sec, 3.792 real sec
[M::mem_process_seqs] Processed 685766 reads in 76.069 CPU sec, 2.397 real sec
[M::process] read 685778 sequences (100000040 bp) in 1.629 CPU sec, 1.693 real sec...
sorting block 27, #reads = 1088830 mem = 536879631 peak = 1007165440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286260, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 281, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (303.47, 145.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685704 sequences (100000076 bp) in 1.816 CPU sec, 1.914 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286304, 3, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 351, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (244.78, 121.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 281, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (303.35, 145.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 685924 reads in 120.128 CPU sec, 3.831 real sec
[M::mem_process_seqs] Processed 685778 reads in 76.350 CPU sec, 2.407 real sec
[M::process] read 685768 sequences (100000054 bp) in 1.618 CPU sec, 1.664 real sec...
sorting block 28, #reads = 1088136 mem = 536882177 peak = 1007165440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286002, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (303.02, 144.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685818 sequences (100000190 bp) in 2.052 CPU sec, 2.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286071, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 280, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (302.81, 145.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685704 reads in 121.882 CPU sec, 3.874 real sec
[M::mem_process_seqs] Processed 685768 reads in 77.665 CPU sec, 2.452 real sec
[M::process] read 685838 sequences (100000004 bp) in 1.514 CPU sec, 1.536 real sec...
sorting block 29, #reads = 1089699 mem = 536878779 peak = 1007165440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286054, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (302.25, 145.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 30, #reads = 1087384 mem = 536878398 peak = 1007165440
[M::process] read 685730 sequences (100000068 bp) in 1.873 CPU sec, 1.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286282, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (301.79, 144.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685818 reads in 121.554 CPU sec, 3.867 real sec
[M::process] read 685836 sequences (100000040 bp) in 1.402 CPU sec, 1.416 real sec...
[M::mem_process_seqs] Processed 685838 reads in 76.953 CPU sec, 2.425 real sec
sorting block 31, #reads = 1088454 mem = 536877882 peak = 1007165440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286179, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.65, 142.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685646 sequences (100000089 bp) in 2.125 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286374, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (297.39, 140.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685730 reads in 121.599 CPU sec, 3.867 real sec
[M::process] read 685736 sequences (100000183 bp) in 1.427 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 685836 reads in 76.822 CPU sec, 2.423 real sec
sorting block 32, #reads = 1087483 mem = 536879347 peak = 1007165440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285962, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (297.04, 140.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685552 sequences (100000040 bp) in 1.893 CPU sec, 2.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286359, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (297.65, 140.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685646 reads in 121.514 CPU sec, 3.875 real sec
[M::mem_process_seqs] Processed 685736 reads in 77.394 CPU sec, 2.434 real sec
[M::process] read 684950 sequences (100000209 bp) in 1.542 CPU sec, 1.572 real sec...
sorting block 33, #reads = 1088349 mem = 536881077 peak = 1007165440
[M::process] read 685184 sequences (100000065 bp) in 2.040 CPU sec, 2.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285476, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (299.11, 140.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684950 reads in 67.448 CPU sec, 2.114 real sec
[M::process] read 685188 sequences (100000102 bp) in 1.416 CPU sec, 1.419 real sec...
sorting block 34, #reads = 1086963 mem = 536879240 peak = 1007165440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286172, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 275, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (297.64, 140.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285721, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (299.29, 140.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685552 reads in 188.846 CPU sec, 5.984 real sec
[M::process] read 684810 sequences (100000002 bp) in 1.813 CPU sec, 1.834 real sec...
[M::mem_process_seqs] Processed 685184 reads in 89.741 CPU sec, 2.835 real sec
sorting block 35, #reads = 1086178 mem = 536883593 peak = 1007165440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286056, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (298.75, 140.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684646 sequences (100000149 bp) in 1.960 CPU sec, 2.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285681, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (300.86, 141.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685188 reads in 120.977 CPU sec, 3.853 real sec
[M::mem_process_seqs] Processed 684810 reads in 76.948 CPU sec, 2.424 real sec
[M::process] read 684772 sequences (100000139 bp) in 1.517 CPU sec, 1.549 real sec...
sorting block 36, #reads = 1087037 mem = 536880571 peak = 1007165440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285862, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (303.54, 143.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (287, 287, 819)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1883)
[M::mem_pestat] mean and std.dev: (535.90, 351.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2415)
[M::mem_pestat] skip orientation RR
sorting block 37, #reads = 1087152 mem = 536875919 peak = 1030868992
[M::process] read 684594 sequences (100000300 bp) in 2.106 CPU sec, 2.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286021, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.01, 142.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684646 reads in 122.430 CPU sec, 3.887 real sec
[M::process] read 684166 sequences (100000113 bp) in 1.447 CPU sec, 1.465 real sec...
[M::mem_process_seqs] Processed 684772 reads in 78.167 CPU sec, 2.463 real sec
sorting block 38, #reads = 1086433 mem = 536878458 peak = 1030868992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285754, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (303.14, 143.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684210 sequences (100000079 bp) in 1.896 CPU sec, 2.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285304, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (304.94, 143.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684594 reads in 122.065 CPU sec, 3.882 real sec
[M::process] read 684562 sequences (100000132 bp) in 1.442 CPU sec, 1.460 real sec...
[M::mem_process_seqs] Processed 684166 reads in 77.419 CPU sec, 2.442 real sec
sorting block 39, #reads = 1086478 mem = 536880809 peak = 1030868992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285810, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 283, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (305.16, 144.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684592 sequences (100000184 bp) in 2.053 CPU sec, 2.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285827, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (306.86, 146.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684210 reads in 123.233 CPU sec, 3.921 real sec
[M::process] read 688148 sequences (100000146 bp) in 1.439 CPU sec, 1.448 real sec...
[M::mem_process_seqs] Processed 684562 reads in 79.060 CPU sec, 2.498 real sec
sorting block 40, #reads = 1086129 mem = 536881132 peak = 1030868992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286188, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (307.62, 147.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689682 sequences (100000089 bp) in 1.906 CPU sec, 2.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286891, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (292.10, 139.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684592 reads in 125.506 CPU sec, 3.997 real sec
[M::process] read 689318 sequences (100000052 bp) in 1.475 CPU sec, 1.484 real sec...
[M::mem_process_seqs] Processed 688148 reads in 79.038 CPU sec, 2.492 real sec
sorting block 41, #reads = 1085709 mem = 536882431 peak = 1030868992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287170, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 264, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (286.99, 138.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1086963 mem = 536880605 peak = 1044832256
[M::process] read 689484 sequences (100000101 bp) in 2.163 CPU sec, 2.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287161, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 266, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (288.08, 137.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689682 reads in 123.846 CPU sec, 3.933 real sec
[M::process] read 689264 sequences (100000219 bp) in 1.471 CPU sec, 1.481 real sec...
[M::mem_process_seqs] Processed 689318 reads in 78.550 CPU sec, 2.480 real sec
sorting block 43, #reads = 1090258 mem = 536878908 peak = 1044832256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287401, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 266, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (288.26, 138.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687940 sequences (100000268 bp) in 1.915 CPU sec, 2.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287747, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 267, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (289.37, 138.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689484 reads in 123.883 CPU sec, 3.946 real sec
[M::process] read 687920 sequences (100000133 bp) in 1.498 CPU sec, 1.521 real sec...
[M::mem_process_seqs] Processed 689264 reads in 78.108 CPU sec, 2.461 real sec
sorting block 44, #reads = 1089443 mem = 536883868 peak = 1044832256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286914, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (292.17, 138.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687348 sequences (100000096 bp) in 2.179 CPU sec, 2.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286685, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (292.70, 138.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687940 reads in 121.806 CPU sec, 3.875 real sec
[M::mem_process_seqs] Processed 687920 reads in 77.161 CPU sec, 2.430 real sec
[M::process] read 686708 sequences (100000013 bp) in 1.561 CPU sec, 1.592 real sec...
sorting block 45, #reads = 1090414 mem = 536880252 peak = 1044832256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286679, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (294.79, 138.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686494 sequences (100000275 bp) in 1.924 CPU sec, 2.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286332, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (296.77, 139.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687348 reads in 121.164 CPU sec, 3.860 real sec
sorting block 46, #reads = 1089219 mem = 536883281 peak = 1044832256
[M::mem_process_seqs] Processed 686708 reads in 77.077 CPU sec, 2.430 real sec
[M::process] read 686144 sequences (100000075 bp) in 1.604 CPU sec, 1.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286393, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (297.65, 140.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1087856 mem = 536877031 peak = 1074417664
[M::process] read 686044 sequences (100000153 bp) in 2.008 CPU sec, 2.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286384, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (297.85, 139.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686494 reads in 122.923 CPU sec, 3.916 real sec
[M::mem_process_seqs] Processed 686144 reads in 78.784 CPU sec, 2.487 real sec
[M::process] read 685910 sequences (100000154 bp) in 1.667 CPU sec, 1.730 real sec...
sorting block 48, #reads = 1088194 mem = 536877910 peak = 1074417664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286472, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 280, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (300.11, 141.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685846 sequences (100000284 bp) in 1.882 CPU sec, 1.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286317, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (300.28, 140.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686044 reads in 124.724 CPU sec, 3.977 real sec
[M::mem_process_seqs] Processed 685910 reads in 78.983 CPU sec, 2.489 real sec
[M::process] read 685912 sequences (100000001 bp) in 1.605 CPU sec, 1.634 real sec...
sorting block 49, #reads = 1087752 mem = 536880873 peak = 1074417664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286359, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (299.82, 141.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685288 sequences (100000256 bp) in 2.166 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286271, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (300.88, 142.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685846 reads in 122.627 CPU sec, 3.900 real sec
[M::mem_process_seqs] Processed 685912 reads in 78.319 CPU sec, 2.471 real sec
[M::process] read 685588 sequences (100000043 bp) in 1.582 CPU sec, 1.616 real sec...
sorting block 50, #reads = 1088152 mem = 536882077 peak = 1074417664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286289, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (302.37, 142.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685896 sequences (100000109 bp) in 2.017 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286316, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (301.75, 142.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685288 reads in 123.781 CPU sec, 3.946 real sec
sorting block 51, #reads = 1087434 mem = 536886372 peak = 1074417664
[M::mem_process_seqs] Processed 685588 reads in 78.736 CPU sec, 2.487 real sec
[M::process] read 685428 sequences (100000064 bp) in 1.689 CPU sec, 1.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285965, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (296.84, 139.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 52, #reads = 1087895 mem = 536877683 peak = 1084829696
[M::process] read 685310 sequences (100000229 bp) in 2.065 CPU sec, 2.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285966, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (298.75, 140.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685896 reads in 125.732 CPU sec, 3.999 real sec
[M::process] read 685334 sequences (100000065 bp) in 1.506 CPU sec, 1.528 real sec...
[M::mem_process_seqs] Processed 685428 reads in 80.196 CPU sec, 2.527 real sec
sorting block 53, #reads = 1088479 mem = 536878869 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286063, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (299.23, 140.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685616 sequences (100000254 bp) in 1.947 CPU sec, 2.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286271, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (298.77, 140.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685310 reads in 126.121 CPU sec, 4.018 real sec
[M::process] read 685640 sequences (100000041 bp) in 1.441 CPU sec, 1.450 real sec...
[M::mem_process_seqs] Processed 685334 reads in 80.611 CPU sec, 2.543 real sec
sorting block 54, #reads = 1086548 mem = 536878702 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285997, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (298.27, 140.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685012 sequences (100000258 bp) in 2.145 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286057, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (298.03, 140.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685318 sequences (100000181 bp) in 1.370 CPU sec, 1.371 real sec...
[M::mem_process_seqs] Processed 685616 reads in 127.615 CPU sec, 4.052 real sec
[M::mem_process_seqs] Processed 685640 reads in 80.880 CPU sec, 2.561 real sec
sorting block 55, #reads = 1086645 mem = 536878282 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285468, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (300.65, 141.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1085942 mem = 536877427 peak = 1084829696
[M::process] read 685034 sequences (100000249 bp) in 2.025 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286121, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (298.38, 139.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685012 reads in 127.490 CPU sec, 4.070 real sec
[M::mem_process_seqs] Processed 685318 reads in 81.506 CPU sec, 2.571 real sec
[M::process] read 685046 sequences (100000125 bp) in 1.604 CPU sec, 1.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285929, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (298.97, 139.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 57, #reads = 1086431 mem = 536878944 peak = 1084829696
[M::process] read 684736 sequences (100000250 bp) in 2.189 CPU sec, 2.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286303, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (300.50, 141.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685034 reads in 128.025 CPU sec, 4.074 real sec
[M::mem_process_seqs] Processed 685046 reads in 81.944 CPU sec, 2.585 real sec
[M::process] read 684944 sequences (100000242 bp) in 1.565 CPU sec, 1.588 real sec...
sorting block 58, #reads = 1086846 mem = 536879106 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285809, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (302.05, 142.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684566 sequences (100000030 bp) in 1.888 CPU sec, 1.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286044, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (301.64, 143.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684954 sequences (100000142 bp) in 1.370 CPU sec, 1.364 real sec...
[M::mem_process_seqs] Processed 684736 reads in 130.124 CPU sec, 4.141 real sec
[M::mem_process_seqs] Processed 684944 reads in 82.769 CPU sec, 2.617 real sec
sorting block 59, #reads = 1085119 mem = 536879339 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285483, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (302.82, 143.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684858 sequences (100000045 bp) in 1.999 CPU sec, 2.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285993, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (301.83, 143.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684566 reads in 127.161 CPU sec, 4.041 real sec
[M::process] read 684848 sequences (100000130 bp) in 1.390 CPU sec, 1.389 real sec...
sorting block 60, #reads = 1084918 mem = 536877722 peak = 1084829696
[M::mem_process_seqs] Processed 684954 reads in 80.380 CPU sec, 2.544 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286125, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (303.12, 144.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1085898 mem = 536878518 peak = 1084829696
[M::process] read 687918 sequences (100000156 bp) in 2.031 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286324, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (301.97, 143.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684858 reads in 127.890 CPU sec, 4.072 real sec
[M::process] read 688948 sequences (100000080 bp) in 1.432 CPU sec, 1.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287223, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (291.32, 138.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688416 sequences (100000288 bp) in 1.470 CPU sec, 1.472 real sec...
[M::mem_process_seqs] Processed 687918 reads in 78.121 CPU sec, 2.552 real sec
sorting block 62, #reads = 1084643 mem = 536878589 peak = 1084829696
[M::mem_process_seqs] Processed 684848 reads in 184.178 CPU sec, 5.915 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286744, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 267, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (288.97, 137.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1087480 mem = 536879900 peak = 1084829696
[M::process] read 688384 sequences (100000180 bp) in 1.911 CPU sec, 2.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286572, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 268, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (290.20, 138.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688948 reads in 137.057 CPU sec, 4.356 real sec
[M::mem_process_seqs] Processed 688416 reads in 79.288 CPU sec, 2.502 real sec
[M::process] read 687698 sequences (100000015 bp) in 1.721 CPU sec, 1.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286677, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 270, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (291.57, 138.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 64, #reads = 1090589 mem = 536881135 peak = 1084829696
[M::process] read 687898 sequences (100000170 bp) in 2.033 CPU sec, 2.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286699, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (292.82, 139.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688384 reads in 126.059 CPU sec, 4.016 real sec
[M::mem_process_seqs] Processed 687698 reads in 80.561 CPU sec, 2.541 real sec
[M::process] read 687116 sequences (100000253 bp) in 1.552 CPU sec, 1.577 real sec...
sorting block 65, #reads = 1089570 mem = 536878361 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287153, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (293.15, 139.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687766 sequences (100000183 bp) in 2.045 CPU sec, 2.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285940, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (294.89, 139.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687898 reads in 126.079 CPU sec, 4.022 real sec
[M::mem_process_seqs] Processed 687116 reads in 80.727 CPU sec, 2.554 real sec
[M::process] read 686692 sequences (100000039 bp) in 1.598 CPU sec, 1.632 real sec...
sorting block 66, #reads = 1089556 mem = 536879677 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286525, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (295.39, 140.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686160 sequences (100000300 bp) in 2.252 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286275, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (297.63, 141.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687766 reads in 126.488 CPU sec, 4.028 real sec
[M::mem_process_seqs] Processed 686692 reads in 80.087 CPU sec, 2.531 real sec
sorting block 67, #reads = 1088627 mem = 536882995 peak = 1084829696
[M::process] read 686480 sequences (100000194 bp) in 1.618 CPU sec, 1.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286495, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (300.20, 142.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1088779 mem = 536877603 peak = 1084829696
[M::process] read 686118 sequences (100000026 bp) in 1.882 CPU sec, 1.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286019, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.29, 141.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686160 reads in 126.424 CPU sec, 4.040 real sec
[M::mem_process_seqs] Processed 686480 reads in 80.893 CPU sec, 2.552 real sec
[M::process] read 686092 sequences (100000136 bp) in 1.653 CPU sec, 1.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286576, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.71, 141.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1088973 mem = 536878178 peak = 1084829696
[M::process] read 685994 sequences (100000270 bp) in 1.880 CPU sec, 1.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285715, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.61, 142.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686118 reads in 126.796 CPU sec, 4.051 real sec
[M::mem_process_seqs] Processed 686092 reads in 82.050 CPU sec, 2.592 real sec
[M::process] read 685982 sequences (100000134 bp) in 2.098 CPU sec, 2.184 real sec...
sorting block 70, #reads = 1087809 mem = 536877139 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285568, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (301.23, 142.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686096 sequences (100000239 bp) in 2.476 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286105, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (300.46, 141.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685994 reads in 136.886 CPU sec, 4.382 real sec
[M::mem_process_seqs] Processed 685982 reads in 86.764 CPU sec, 2.747 real sec
sorting block 71, #reads = 1087930 mem = 536879724 peak = 1084829696
[M::process] read 686128 sequences (100000072 bp) in 2.688 CPU sec, 2.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286388, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (300.84, 143.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286520, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (300.89, 143.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686096 reads in 137.694 CPU sec, 4.435 real sec
[M::process] read 688020 sequences (100000061 bp) in 2.681 CPU sec, 2.875 real sec...
[M::mem_process_seqs] Processed 686128 reads in 86.947 CPU sec, 2.775 real sec
sorting block 72, #reads = 1087626 mem = 536879003 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287097, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 265, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (288.52, 138.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688020 reads in 75.707 CPU sec, 2.529 real sec
[M::process] read 686254 sequences (100000040 bp) in 2.768 CPU sec, 2.979 real sec...
sorting block 73, #reads = 1086466 mem = 536879410 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286110, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 272, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (294.03, 138.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686254 reads in 76.085 CPU sec, 2.522 real sec
[M::process] read 686492 sequences (100000178 bp) in 2.784 CPU sec, 3.006 real sec...
sorting block 74, #reads = 1087847 mem = 536879176 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286471, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 271, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (293.50, 138.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686492 reads in 76.436 CPU sec, 2.512 real sec
[M::process] read 685920 sequences (100000228 bp) in 2.939 CPU sec, 3.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285985, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 273, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (295.33, 139.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685920 reads in 76.167 CPU sec, 2.474 real sec
sorting block 75, #reads = 1086782 mem = 536879230 peak = 1084829696
[M::process] read 685850 sequences (100000213 bp) in 2.794 CPU sec, 3.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285869, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 274, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (295.28, 138.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685850 reads in 75.636 CPU sec, 2.453 real sec
[M::process] read 685432 sequences (100000207 bp) in 2.716 CPU sec, 2.923 real sec...
sorting block 76, #reads = 1086750 mem = 536879673 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285702, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 275, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (294.93, 136.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685432 reads in 74.923 CPU sec, 2.428 real sec
[M::process] read 685852 sequences (100000054 bp) in 2.327 CPU sec, 2.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285760, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 274, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (295.13, 138.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685852 reads in 75.017 CPU sec, 2.439 real sec
sorting block 77, #reads = 1087166 mem = 536880437 peak = 1084829696
[M::process] read 685446 sequences (100000214 bp) in 2.665 CPU sec, 2.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286065, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 275, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (295.64, 137.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685446 reads in 74.257 CPU sec, 2.405 real sec
[M::process] read 685526 sequences (100000154 bp) in 2.340 CPU sec, 2.468 real sec...
sorting block 78, #reads = 1087315 mem = 536880726 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286360, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 275, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (294.51, 136.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685526 reads in 74.923 CPU sec, 2.434 real sec
[M::process] read 685276 sequences (100000070 bp) in 2.687 CPU sec, 2.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286078, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 275, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (294.46, 135.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685276 reads in 73.871 CPU sec, 2.397 real sec
[M::process] read 684980 sequences (100000010 bp) in 2.360 CPU sec, 2.480 real sec...
sorting block 79, #reads = 1086216 mem = 536879240 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285560, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (297.47, 138.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684980 reads in 75.220 CPU sec, 2.437 real sec
[M::process] read 685164 sequences (100000043 bp) in 2.813 CPU sec, 3.016 real sec...
sorting block 80, #reads = 1085682 mem = 536882787 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286055, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (299.09, 140.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685204 sequences (100000079 bp) in 2.152 CPU sec, 2.248 real sec...
[M::mem_process_seqs] Processed 685164 reads in 74.842 CPU sec, 2.421 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286280, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (299.71, 141.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685204 reads in 74.442 CPU sec, 2.414 real sec
[M::process] read 684866 sequences (100000134 bp) in 2.471 CPU sec, 2.628 real sec...
sorting block 81, #reads = 1085822 mem = 536878425 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285569, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (300.84, 141.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684866 reads in 74.062 CPU sec, 2.398 real sec
[M::process] read 684824 sequences (100000057 bp) in 2.362 CPU sec, 2.696 real sec...
sorting block 82, #reads = 1086759 mem = 536881306 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285827, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (303.08, 143.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684824 reads in 72.916 CPU sec, 2.359 real sec
[M::process] read 684418 sequences (100000292 bp) in 2.258 CPU sec, 2.380 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285494, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (303.99, 143.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690572 sequences (100000137 bp) in 2.063 CPU sec, 2.155 real sec...
[M::mem_process_seqs] Processed 684418 reads in 72.882 CPU sec, 2.353 real sec
sorting block 83, #reads = 1084952 mem = 536880252 peak = 1084829696
[M::process] read 693152 sequences (100000251 bp) in 2.086 CPU sec, 2.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288234, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.90, 137.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 692284 sequences (100000061 bp) in 1.420 CPU sec, 1.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288805, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 244, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (271.30, 133.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690572 reads in 141.782 CPU sec, 4.459 real sec
[M::mem_process_seqs] Processed 693152 reads in 87.762 CPU sec, 2.754 real sec
[M::process] read 690522 sequences (100000081 bp) in 1.522 CPU sec, 1.559 real sec...
sorting block 84, #reads = 1086417 mem = 536881271 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289195, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 249, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (276.74, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689348 sequences (100000007 bp) in 2.071 CPU sec, 2.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288123, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 257, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (284.24, 139.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688784 sequences (100000123 bp) in 1.330 CPU sec, 1.329 real sec...
[M::mem_process_seqs] Processed 692284 reads in 138.583 CPU sec, 4.415 real sec
[M::mem_process_seqs] Processed 690522 reads in 85.244 CPU sec, 2.699 real sec
sorting block 85, #reads = 1089320 mem = 536882813 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287398, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 262, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (288.44, 141.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688544 sequences (100000193 bp) in 1.901 CPU sec, 2.002 real sec...
[M::process] read 688070 sequences (100000127 bp) in 1.340 CPU sec, 1.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287447, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (290.83, 142.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689348 reads in 133.371 CPU sec, 4.238 real sec
sorting block 86, #reads = 1089246 mem = 536878791 peak = 1084829696
[M::mem_process_seqs] Processed 688784 reads in 83.782 CPU sec, 2.663 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286910, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (292.74, 143.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 87, #reads = 1089115 mem = 536878229 peak = 1084829696
[M::process] read 687940 sequences (100000038 bp) in 2.146 CPU sec, 2.296 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286894, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (292.98, 142.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687526 sequences (100000220 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_process_seqs] Processed 688544 reads in 134.397 CPU sec, 4.272 real sec
[M::mem_process_seqs] Processed 688070 reads in 84.836 CPU sec, 2.690 real sec
sorting block 88, #reads = 1088404 mem = 536885514 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286675, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (295.03, 143.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687318 sequences (100000263 bp) in 2.099 CPU sec, 2.201 real sec...
[M::process] read 687242 sequences (100000139 bp) in 1.370 CPU sec, 1.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286953, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (296.23, 143.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687940 reads in 134.756 CPU sec, 4.279 real sec
[M::mem_process_seqs] Processed 687526 reads in 83.744 CPU sec, 2.657 real sec
sorting block 89, #reads = 1087156 mem = 536876303 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286871, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (297.28, 144.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686840 sequences (100000043 bp) in 2.160 CPU sec, 2.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287206, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (298.60, 145.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686686 sequences (100000198 bp) in 1.400 CPU sec, 1.405 real sec...
[M::mem_process_seqs] Processed 687318 reads in 134.587 CPU sec, 4.275 real sec
[M::mem_process_seqs] Processed 687242 reads in 84.924 CPU sec, 2.689 real sec
sorting block 90, #reads = 1087823 mem = 536879136 peak = 1084829696
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286318, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (299.13, 144.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686402 sequences (100000241 bp) in 2.189 CPU sec, 2.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286866, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (297.94, 142.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686152 sequences (100000017 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 686840 reads in 137.217 CPU sec, 4.361 real sec
sorting block 91, #reads = 1086668 mem = 536877444 peak = 1084829696
[M::mem_process_seqs] Processed 686686 reads in 86.514 CPU sec, 2.739 real sec
sorting block 92, #reads = 1086138 mem = 536879474 peak = 1114873856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286876, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (299.75, 144.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686008 sequences (100000092 bp) in 2.261 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286361, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.77, 142.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685916 sequences (100000257 bp) in 1.430 CPU sec, 1.428 real sec...
[M::mem_process_seqs] Processed 686402 reads in 137.810 CPU sec, 4.378 real sec
[M::mem_process_seqs] Processed 686152 reads in 86.634 CPU sec, 2.743 real sec
sorting block 93, #reads = 1085371 mem = 536877923 peak = 1114873856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286923, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (299.99, 143.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686012 sequences (100000236 bp) in 2.136 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286328, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (299.12, 142.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686310 sequences (100000259 bp) in 1.450 CPU sec, 1.451 real sec...
[M::mem_process_seqs] Processed 686008 reads in 137.154 CPU sec, 4.362 real sec
[M::mem_process_seqs] Processed 685916 reads in 87.050 CPU sec, 2.757 real sec
sorting block 94, #reads = 1085121 mem = 536877735 peak = 1114873856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286389, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (298.24, 142.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685838 sequences (100000276 bp) in 2.285 CPU sec, 2.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286467, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (297.78, 142.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685958 sequences (100000144 bp) in 1.440 CPU sec, 1.437 real sec...
[M::mem_process_seqs] Processed 686012 reads in 139.375 CPU sec, 4.432 real sec
sorting block 95, #reads = 1085557 mem = 536877982 peak = 1114873856
[M::mem_process_seqs] Processed 686310 reads in 87.374 CPU sec, 2.769 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286404, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (298.16, 141.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 96, #reads = 1084525 mem = 536879334 peak = 1114873856
[M::process] read 685798 sequences (100000244 bp) in 2.388 CPU sec, 2.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286458, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (298.06, 141.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685414 sequences (100000159 bp) in 1.470 CPU sec, 1.466 real sec...
[M::mem_process_seqs] Processed 685838 reads in 139.745 CPU sec, 4.436 real sec
[M::mem_process_seqs] Processed 685958 reads in 88.110 CPU sec, 2.789 real sec
sorting block 97, #reads = 1085256 mem = 536880385 peak = 1114873856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286589, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (299.02, 142.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685740 sequences (100000060 bp) in 2.316 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285682, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (298.33, 141.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685486 sequences (100000162 bp) in 1.440 CPU sec, 1.438 real sec...
[M::mem_process_seqs] Processed 685798 reads in 139.801 CPU sec, 4.441 real sec
[M::mem_process_seqs] Processed 685414 reads in 88.611 CPU sec, 2.804 real sec
sorting block 98, #reads = 1084321 mem = 536889147 peak = 1114873856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286302, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (298.93, 141.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685164 sequences (100000049 bp) in 2.411 CPU sec, 2.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286116, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (298.59, 141.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685740 reads in 139.830 CPU sec, 4.444 real sec
[M::process] read 685646 sequences (100000210 bp) in 1.450 CPU sec, 1.446 real sec...
[M::mem_process_seqs] Processed 685486 reads in 87.551 CPU sec, 2.773 real sec
sorting block 99, #reads = 1083356 mem = 536878745 peak = 1114873856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286462, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (300.65, 141.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685318 sequences (100000160 bp) in 2.430 CPU sec, 2.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286166, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (298.76, 141.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685058 sequences (100000044 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 685164 reads in 138.114 CPU sec, 4.384 real sec
sorting block 100, #reads = 1085030 mem = 536878284 peak = 1114873856
[M::mem_process_seqs] Processed 685646 reads in 87.207 CPU sec, 2.761 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286576, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (301.26, 143.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 101, #reads = 1084266 mem = 536878273 peak = 1114873856
[M::process] read 684888 sequences (100000031 bp) in 2.351 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285981, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (302.65, 144.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687294 sequences (100000233 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 685318 reads in 138.687 CPU sec, 4.403 real sec
[M::mem_process_seqs] Processed 685058 reads in 87.588 CPU sec, 2.772 real sec
sorting block 102, #reads = 1084181 mem = 536876654 peak = 1114873856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285845, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (303.08, 144.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696206 sequences (100000257 bp) in 2.384 CPU sec, 2.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286465, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (294.15, 142.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695040 sequences (100000114 bp) in 1.480 CPU sec, 1.479 real sec...
[M::mem_process_seqs] Processed 684888 reads in 143.080 CPU sec, 4.543 real sec
[M::mem_process_seqs] Processed 687294 reads in 91.577 CPU sec, 2.898 real sec
sorting block 103, #reads = 1083164 mem = 536878451 peak = 1114873856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289811, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 235, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (260.81, 126.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 694932 sequences (100000064 bp) in 2.390 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 289888, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 238, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (264.61, 128.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 693202 sequences (100000261 bp) in 1.500 CPU sec, 1.495 real sec...
[M::mem_process_seqs] Processed 696206 reads in 148.040 CPU sec, 4.704 real sec
[M::mem_process_seqs] Processed 695040 reads in 92.223 CPU sec, 2.919 real sec
sorting block 104, #reads = 1084977 mem = 536878442 peak = 1114873856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 289713, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 240, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (265.35, 128.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 886)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 692150 sequences (100000237 bp) in 2.391 CPU sec, 2.522 real sec...
[M::process] read 691430 sequences (100000208 bp) in 1.460 CPU sec, 1.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288915, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 245, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (270.42, 130.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694932 reads in 150.368 CPU sec, 4.778 real sec
sorting block 105, #reads = 1088206 mem = 536878028 peak = 1114873856
[M::mem_process_seqs] Processed 693202 reads in 93.027 CPU sec, 2.950 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 289001, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 250, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (274.80, 132.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 106, #reads = 1084621 mem = 536880416 peak = 1127903232
[M::process] read 691590 sequences (100000137 bp) in 3.017 CPU sec, 3.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288505, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 253, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (278.12, 134.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 692150 reads in 146.171 CPU sec, 4.655 real sec
[M::mem_process_seqs] Processed 691430 reads in 92.457 CPU sec, 2.918 real sec
[M::process] read 690632 sequences (100000026 bp) in 2.030 CPU sec, 2.105 real sec...
sorting block 107, #reads = 1084882 mem = 536879154 peak = 1127903232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288400, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 253, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755)
[M::mem_pestat] mean and std.dev: (279.25, 136.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690056 sequences (100000031 bp) in 2.232 CPU sec, 2.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288458, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 257, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (282.30, 136.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691590 reads in 145.765 CPU sec, 4.634 real sec
[M::process] read 690080 sequences (100000208 bp) in 1.430 CPU sec, 1.431 real sec...
[M::mem_process_seqs] Processed 690632 reads in 91.092 CPU sec, 2.883 real sec
sorting block 108, #reads = 1084636 mem = 536878597 peak = 1127903232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287751, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 259, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (284.79, 138.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689922 sequences (100000205 bp) in 2.286 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288564, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 259, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (284.87, 138.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689096 sequences (100000211 bp) in 1.470 CPU sec, 1.471 real sec...
[M::mem_process_seqs] Processed 690056 reads in 141.892 CPU sec, 4.512 real sec
sorting block 109, #reads = 1085616 mem = 536879584 peak = 1127903232
[M::mem_process_seqs] Processed 690080 reads in 89.058 CPU sec, 2.816 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287615, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 261, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (285.66, 137.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 110, #reads = 1086855 mem = 536881119 peak = 1127903232
[M::process] read 688690 sequences (100000259 bp) in 2.288 CPU sec, 2.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287457, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (288.38, 138.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688230 sequences (100000293 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 689922 reads in 142.724 CPU sec, 4.538 real sec
[M::mem_process_seqs] Processed 689096 reads in 89.282 CPU sec, 2.828 real sec
sorting block 111, #reads = 1084215 mem = 536877355 peak = 1127903232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286722, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 267, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (290.80, 139.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687688 sequences (100000060 bp) in 2.441 CPU sec, 2.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287641, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 268, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (292.73, 141.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687394 sequences (100000291 bp) in 1.480 CPU sec, 1.484 real sec...
[M::mem_process_seqs] Processed 688690 reads in 141.078 CPU sec, 4.477 real sec
[M::mem_process_seqs] Processed 688230 reads in 88.864 CPU sec, 2.812 real sec
sorting block 112, #reads = 1084236 mem = 536877284 peak = 1127903232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287125, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (294.69, 141.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686768 sequences (100000048 bp) in 2.296 CPU sec, 2.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286817, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (295.77, 142.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686650 sequences (100000171 bp) in 1.400 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 687688 reads in 139.915 CPU sec, 4.442 real sec
[M::mem_process_seqs] Processed 687394 reads in 88.045 CPU sec, 2.794 real sec
sorting block 113, #reads = 1084295 mem = 536878024 peak = 1127903232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287014, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 271, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (295.28, 140.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686584 sequences (100000120 bp) in 2.193 CPU sec, 2.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286628, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (296.24, 141.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686288 sequences (100000250 bp) in 1.400 CPU sec, 1.400 real sec...
[M::mem_process_seqs] Processed 686768 reads in 139.390 CPU sec, 4.433 real sec
sorting block 114, #reads = 1084193 mem = 536878626 peak = 1127903232
[M::mem_process_seqs] Processed 686650 reads in 87.811 CPU sec, 2.777 real sec
sorting block 115, #reads = 1084177 mem = 536883289 peak = 1127903232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286636, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 272, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (296.09, 141.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686072 sequences (100000173 bp) in 2.248 CPU sec, 2.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286575, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (297.44, 142.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685988 sequences (100000183 bp) in 1.390 CPU sec, 1.385 real sec...
[M::mem_process_seqs] Processed 686584 reads in 135.086 CPU sec, 4.289 real sec
[M::mem_process_seqs] Processed 686288 reads in 84.673 CPU sec, 2.677 real sec
sorting block 116, #reads = 1084237 mem = 536879714 peak = 1127903232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286079, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (298.08, 142.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686196 sequences (100000078 bp) in 2.128 CPU sec, 2.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286745, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 274, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (297.81, 142.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686072 reads in 131.202 CPU sec, 4.172 real sec
[M::process] read 685650 sequences (100000198 bp) in 1.480 CPU sec, 1.484 real sec...
[M::mem_process_seqs] Processed 685988 reads in 82.945 CPU sec, 2.614 real sec
sorting block 117, #reads = 1085088 mem = 536878563 peak = 1127903232
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286504, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (296.73, 141.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685104 sequences (100000086 bp) in 2.279 CPU sec, 2.384 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286395, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.35, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684960 sequences (100000015 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_process_seqs] Processed 686196 reads in 133.720 CPU sec, 4.245 real sec
sorting block 118, #reads = 1084204 mem = 536877615 peak = 1127903232
[M::mem_process_seqs] Processed 685650 reads in 84.333 CPU sec, 2.666 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285935, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (300.76, 142.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685160 sequences (100000171 bp) in 2.196 CPU sec, 2.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286455, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (301.47, 142.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685104 reads in 130.865 CPU sec, 4.163 real sec
sorting block 119, #reads = 1084175 mem = 536878501 peak = 1127903232
[M::process] read 685064 sequences (100000031 bp) in 1.505 CPU sec, 1.522 real sec...
[M::mem_process_seqs] Processed 684960 reads in 83.000 CPU sec, 2.621 real sec
sorting block 120, #reads = 1085308 mem = 536878551 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285885, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (302.02, 144.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686288 sequences (100000046 bp) in 2.236 CPU sec, 2.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285866, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (303.15, 145.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685160 reads in 133.265 CPU sec, 4.237 real sec
[M::process] read 691206 sequences (100000278 bp) in 1.466 CPU sec, 1.471 real sec...
[M::mem_process_seqs] Processed 685064 reads in 85.248 CPU sec, 2.688 real sec
sorting block 121, #reads = 1084898 mem = 536881514 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286260, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 271, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (296.21, 143.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 693438 sequences (100000043 bp) in 2.424 CPU sec, 2.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288099, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 253, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (279.64, 136.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 692352 sequences (100000012 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_process_seqs] Processed 686288 reads in 144.537 CPU sec, 4.593 real sec
[M::mem_process_seqs] Processed 691206 reads in 93.905 CPU sec, 2.976 real sec
sorting block 122, #reads = 1082712 mem = 536878157 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289038, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 245, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (271.26, 132.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691390 sequences (100000074 bp) in 2.392 CPU sec, 2.542 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288456, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (276.19, 134.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 693438 reads in 132.346 CPU sec, 4.204 real sec
[M::process] read 690856 sequences (100000196 bp) in 1.424 CPU sec, 1.438 real sec...
[M::mem_process_seqs] Processed 692352 reads in 82.860 CPU sec, 2.614 real sec
sorting block 123, #reads = 1082920 mem = 536880990 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288199, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 253, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (278.67, 135.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 124, #reads = 1091023 mem = 536881125 peak = 1152819200
[M::process] read 689922 sequences (100000059 bp) in 2.030 CPU sec, 2.144 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287950, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 255, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.31, 135.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691390 reads in 131.502 CPU sec, 4.201 real sec
[M::process] read 689642 sequences (100000194 bp) in 1.560 CPU sec, 1.577 real sec...
[M::mem_process_seqs] Processed 690856 reads in 82.618 CPU sec, 2.609 real sec
sorting block 125, #reads = 1089878 mem = 536880595 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287613, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 259, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (282.86, 135.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689088 sequences (100000050 bp) in 2.217 CPU sec, 2.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287614, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 261, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (285.05, 136.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689922 reads in 129.221 CPU sec, 4.108 real sec
[M::process] read 688804 sequences (100000069 bp) in 1.415 CPU sec, 1.417 real sec...
[M::mem_process_seqs] Processed 689642 reads in 81.324 CPU sec, 2.570 real sec
sorting block 126, #reads = 1089712 mem = 536877542 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286805, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 263, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.17, 136.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688444 sequences (100000059 bp) in 2.242 CPU sec, 2.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287305, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 265, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (287.56, 136.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 197, 197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 319)
[M::mem_pestat] mean and std.dev: (168.78, 41.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 380)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 689088 reads in 129.757 CPU sec, 4.127 real sec
[M::process] read 688108 sequences (100000193 bp) in 1.472 CPU sec, 1.471 real sec...
[M::mem_process_seqs] Processed 688804 reads in 81.996 CPU sec, 2.587 real sec
sorting block 127, #reads = 1088928 mem = 536879975 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287621, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (289.78, 138.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689072 sequences (100000129 bp) in 2.239 CPU sec, 2.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287218, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 269, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (291.28, 139.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688736 sequences (100000240 bp) in 1.370 CPU sec, 1.372 real sec...
[M::mem_process_seqs] Processed 688444 reads in 136.331 CPU sec, 4.334 real sec
sorting block 128, #reads = 1088597 mem = 536879759 peak = 1152819200
[M::mem_process_seqs] Processed 688108 reads in 85.416 CPU sec, 2.706 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287390, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (289.18, 138.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 129, #reads = 1085837 mem = 536879985 peak = 1152819200
[M::process] read 688316 sequences (100000149 bp) in 2.287 CPU sec, 2.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286707, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (289.84, 139.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689072 reads in 131.266 CPU sec, 4.166 real sec
[M::process] read 687840 sequences (100000288 bp) in 1.420 CPU sec, 1.415 real sec...
[M::mem_process_seqs] Processed 688736 reads in 82.652 CPU sec, 2.613 real sec
sorting block 130, #reads = 1088138 mem = 536880478 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287192, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 269, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (290.78, 138.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687680 sequences (100000025 bp) in 2.215 CPU sec, 2.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286639, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (292.00, 138.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688902 sequences (100000102 bp) in 1.380 CPU sec, 1.376 real sec...
[M::mem_process_seqs] Processed 688316 reads in 131.215 CPU sec, 4.169 real sec
[M::mem_process_seqs] Processed 687840 reads in 82.796 CPU sec, 2.616 real sec
sorting block 131, #reads = 1087827 mem = 536879128 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 287007, 7, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (397, 4560, 4560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12886)
[M::mem_pestat] mean and std.dev: (2446.81, 2114.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17049)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (292.10, 138.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687760 sequences (100000109 bp) in 2.009 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287211, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 263, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (285.42, 135.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687680 reads in 132.494 CPU sec, 4.225 real sec
[M::process] read 686844 sequences (100000235 bp) in 1.483 CPU sec, 1.490 real sec...
[M::mem_process_seqs] Processed 688902 reads in 84.185 CPU sec, 2.654 real sec
sorting block 132, #reads = 1087580 mem = 536878956 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287110, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (292.30, 139.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686778 sequences (100000268 bp) in 2.141 CPU sec, 2.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286759, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (296.17, 140.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687058 sequences (100000235 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_process_seqs] Processed 687760 reads in 135.325 CPU sec, 4.304 real sec
sorting block 133, #reads = 1087591 mem = 536880115 peak = 1152819200
[M::mem_process_seqs] Processed 686844 reads in 85.303 CPU sec, 2.696 real sec
sorting block 134, #reads = 1086922 mem = 536879068 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286488, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (296.63, 141.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686668 sequences (100000282 bp) in 2.430 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287102, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (295.66, 140.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686484 sequences (100000136 bp) in 1.430 CPU sec, 1.427 real sec...
[M::mem_process_seqs] Processed 686778 reads in 137.578 CPU sec, 4.368 real sec
[M::mem_process_seqs] Processed 687058 reads in 87.309 CPU sec, 2.761 real sec
sorting block 135, #reads = 1084807 mem = 536879760 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287152, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (297.30, 141.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686906 sequences (100000141 bp) in 2.251 CPU sec, 2.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286646, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (296.31, 141.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686358 sequences (100000048 bp) in 1.390 CPU sec, 1.384 real sec...
[M::mem_process_seqs] Processed 686668 reads in 137.339 CPU sec, 4.359 real sec
[M::mem_process_seqs] Processed 686484 reads in 86.027 CPU sec, 2.725 real sec
sorting block 136, #reads = 1085088 mem = 536878599 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287019, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 270, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (293.34, 139.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685956 sequences (100000064 bp) in 2.277 CPU sec, 2.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286281, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 272, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (295.03, 139.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686384 sequences (100000160 bp) in 1.420 CPU sec, 1.418 real sec...
[M::mem_process_seqs] Processed 686906 reads in 140.876 CPU sec, 4.478 real sec
sorting block 137, #reads = 1084120 mem = 536881434 peak = 1152819200
[M::mem_process_seqs] Processed 686358 reads in 88.719 CPU sec, 2.806 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285974, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (297.20, 142.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 138, #reads = 1082817 mem = 536880711 peak = 1152819200
[M::process] read 686040 sequences (100000077 bp) in 2.295 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286616, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (297.28, 142.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686124 sequences (100000239 bp) in 1.400 CPU sec, 1.408 real sec...
[M::mem_process_seqs] Processed 685956 reads in 138.188 CPU sec, 4.388 real sec
[M::mem_process_seqs] Processed 686384 reads in 87.189 CPU sec, 2.762 real sec
sorting block 139, #reads = 1083341 mem = 536878883 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286618, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (297.70, 141.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685980 sequences (100000141 bp) in 2.242 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286354, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (297.01, 142.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686610 sequences (100000059 bp) in 1.450 CPU sec, 1.455 real sec...
[M::mem_process_seqs] Processed 686040 reads in 141.831 CPU sec, 4.506 real sec
[M::mem_process_seqs] Processed 686124 reads in 89.351 CPU sec, 2.830 real sec
sorting block 140, #reads = 1083504 mem = 536879886 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286342, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (297.28, 141.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 692556 sequences (100000286 bp) in 2.331 CPU sec, 2.453 real sec...
[M::process] read 697234 sequences (100000092 bp) in 1.440 CPU sec, 1.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285924, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 272, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (295.81, 141.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685980 reads in 148.262 CPU sec, 4.713 real sec
[M::mem_process_seqs] Processed 686610 reads in 97.162 CPU sec, 3.081 real sec
sorting block 141, #reads = 1082073 mem = 536878963 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288573, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 248, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (274.41, 134.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695844 sequences (100000213 bp) in 2.304 CPU sec, 2.381 real sec...
[M::process] read 694336 sequences (100000238 bp) in 1.450 CPU sec, 1.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290100, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (258.70, 125.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 692556 reads in 153.302 CPU sec, 4.871 real sec
sorting block 142, #reads = 1077617 mem = 536878502 peak = 1152819200
[M::mem_process_seqs] Processed 697234 reads in 93.545 CPU sec, 2.972 real sec
sorting block 143, #reads = 1082515 mem = 536883264 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289640, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 239, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (264.22, 128.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 693492 sequences (100000141 bp) in 2.292 CPU sec, 2.386 real sec...
[M::process] read 692556 sequences (100000230 bp) in 1.440 CPU sec, 1.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289546, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 244, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720)
[M::mem_pestat] mean and std.dev: (269.05, 129.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 904)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695844 reads in 146.187 CPU sec, 4.642 real sec
[M::mem_process_seqs] Processed 694336 reads in 90.742 CPU sec, 2.880 real sec
sorting block 144, #reads = 1087117 mem = 536881928 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288704, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 247, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (271.30, 130.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 913)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691982 sequences (100000156 bp) in 2.434 CPU sec, 2.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288883, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 251, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (274.51, 131.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690940 sequences (100000171 bp) in 1.450 CPU sec, 1.452 real sec...
[M::mem_process_seqs] Processed 693492 reads in 146.328 CPU sec, 4.652 real sec
[M::mem_process_seqs] Processed 692556 reads in 92.763 CPU sec, 2.937 real sec
sorting block 145, #reads = 1085754 mem = 536877168 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288878, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 253, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (277.34, 133.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690510 sequences (100000202 bp) in 2.463 CPU sec, 2.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288441, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 257, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (281.14, 134.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689692 sequences (100000290 bp) in 1.410 CPU sec, 1.412 real sec...
[M::mem_process_seqs] Processed 691982 reads in 144.420 CPU sec, 4.585 real sec
sorting block 146, #reads = 1083931 mem = 536882837 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288005, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 259, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (282.45, 135.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690034 sequences (100000034 bp) in 1.880 CPU sec, 1.889 real sec...
[M::mem_process_seqs] Processed 690510 reads in 78.955 CPU sec, 2.496 real sec
sorting block 147, #reads = 1084609 mem = 536880044 peak = 1152819200
[M::mem_process_seqs] Processed 690940 reads in 188.166 CPU sec, 5.967 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287764, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 263, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (286.73, 137.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690128 sequences (100000288 bp) in 2.299 CPU sec, 2.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287631, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 261, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (285.20, 137.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689388 sequences (100000237 bp) in 1.420 CPU sec, 1.423 real sec...
[M::mem_process_seqs] Processed 689692 reads in 156.561 CPU sec, 4.968 real sec
[M::mem_process_seqs] Processed 690034 reads in 92.717 CPU sec, 2.935 real sec
sorting block 148, #reads = 1085766 mem = 536878410 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288171, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 260, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (285.01, 137.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688904 sequences (100000162 bp) in 2.095 CPU sec, 2.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287557, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 263, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (288.43, 139.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690906 sequences (100000106 bp) in 1.500 CPU sec, 1.505 real sec...
[M::mem_process_seqs] Processed 690128 reads in 142.700 CPU sec, 4.543 real sec
sorting block 149, #reads = 1082763 mem = 536889782 peak = 1152819200
[M::mem_process_seqs] Processed 689388 reads in 90.137 CPU sec, 2.852 real sec
sorting block 150, #reads = 1084313 mem = 536881647 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287845, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (289.32, 138.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687892 sequences (100000090 bp) in 2.217 CPU sec, 2.319 real sec...
[M::process] read 687758 sequences (100000010 bp) in 1.450 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288512, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 253, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (277.94, 133.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688904 reads in 141.352 CPU sec, 4.488 real sec
[M::mem_process_seqs] Processed 690906 reads in 88.628 CPU sec, 2.810 real sec
sorting block 151, #reads = 1084611 mem = 536878194 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286829, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 267, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (288.91, 136.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686524 sequences (100000056 bp) in 2.007 CPU sec, 2.144 real sec...
[M::process] read 686520 sequences (100000106 bp) in 1.420 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287160, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (290.28, 137.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687892 reads in 142.879 CPU sec, 4.548 real sec
[M::mem_process_seqs] Processed 687758 reads in 89.345 CPU sec, 2.832 real sec
sorting block 152, #reads = 1084890 mem = 536879953 peak = 1152819200
[M::process] read 686128 sequences (100000240 bp) in 2.308 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286890, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (294.22, 138.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686358 sequences (100000104 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_process_seqs] Processed 686520 reads in 81.188 CPU sec, 2.572 real sec
sorting block 153, #reads = 1083693 mem = 536881146 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287057, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 272, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (293.70, 138.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286261, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 272, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (293.86, 137.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686524 reads in 214.478 CPU sec, 6.816 real sec
[M::process] read 686336 sequences (100000053 bp) in 1.707 CPU sec, 1.721 real sec...
[M::mem_process_seqs] Processed 686128 reads in 100.564 CPU sec, 3.205 real sec
sorting block 154, #reads = 1083481 mem = 536879883 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286226, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 271, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (293.26, 137.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685640 sequences (100000061 bp) in 2.269 CPU sec, 2.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286508, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 270, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (291.01, 135.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686358 reads in 137.196 CPU sec, 4.382 real sec
[M::mem_process_seqs] Processed 686336 reads in 87.081 CPU sec, 2.750 real sec
[M::process] read 685504 sequences (100000083 bp) in 1.638 CPU sec, 1.689 real sec...
sorting block 155, #reads = 1083744 mem = 536879175 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286239, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 272, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (293.06, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685590 sequences (100000062 bp) in 2.061 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286292, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 273, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (294.84, 137.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685640 reads in 136.147 CPU sec, 4.340 real sec
[M::process] read 685302 sequences (100000144 bp) in 1.390 CPU sec, 1.386 real sec...
sorting block 156, #reads = 1084131 mem = 536878816 peak = 1152819200
[M::mem_process_seqs] Processed 685504 reads in 85.625 CPU sec, 2.712 real sec
sorting block 157, #reads = 1083790 mem = 536878056 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286025, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 273, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (293.64, 136.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684964 sequences (100000151 bp) in 1.950 CPU sec, 2.068 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286389, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (299.95, 141.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684772 sequences (100000172 bp) in 1.400 CPU sec, 1.394 real sec...
[M::mem_process_seqs] Processed 685590 reads in 133.613 CPU sec, 4.250 real sec
[M::mem_process_seqs] Processed 685302 reads in 83.787 CPU sec, 2.653 real sec
sorting block 158, #reads = 1083986 mem = 536878155 peak = 1152819200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286337, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (300.60, 142.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684558 sequences (100000130 bp) in 2.142 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286012, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (301.50, 141.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685176 sequences (100000220 bp) in 1.420 CPU sec, 1.420 real sec...
[M::mem_process_seqs] Processed 684964 reads in 131.198 CPU sec, 4.168 real sec
[M::mem_process_seqs] Processed 684772 reads in 82.690 CPU sec, 2.615 real sec
sorting block 159, #reads = 1085605 mem = 536879427 peak = 1152819200
[M::process] read 88290 sequences (12852333 bp) in 0.421 CPU sec, 0.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286122, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (303.67, 144.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 36965, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 272, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (293.40, 139.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286173, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (302.28, 144.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684558 reads in 138.977 CPU sec, 4.507 real sec
[M::mem_process_seqs] Processed 88290 reads in 81.115 CPU sec, 2.620 real sec
sorting block 160, #reads = 1086122 mem = 536879503 peak = 1152819200
[M::mem_process_seqs] Processed 685176 reads in 93.528 CPU sec, 3.042 real sec
sorting block 161, #reads = 1083144 mem = 536880003 peak = 1202925568
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 175293858
overall: 69994573824 mem 19441.017 user 404.158 sys 1607.627 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cfy-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:659_cfy-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cfy-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cfy-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cfy-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1607.661 sec; CPU: 19845.178 sec
sorting block 162, #reads = 154644 mem = 76913817 peak = 1202925568
uniq header count 1
merging 163 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 9392371998
output reads: 176165540
bam_mem_sort: 163 calls 64.269 user 0.810 sys 65.527 real
bam_write: 163 calls 98.260 user 2.481 sys 101.371 real
execute: 1 calls 31.164 user 1.197 sys 1659.160 real
merge_files: 1 calls 31.139 user 1.183 sys 51.333 real
parse_chunk: 2595 calls 83.399 user 24.241 sys 109.744 real
read_chunk: 6997 calls 68.228 user 61.309 sys 616.924 real
sort_block: 1 calls 51.448 user 60.786 sys 594.091 real
write_chunk: 4400 calls 30.732 user 0.943 sys 50.429 real
overall: 3269529600 mem 2012.393 user 100.776 sys 1659.182 real
ls: cannot access '*.cram': No such file or directory