cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1646.470 CPU sec, 894.517 real sec
[M::process] read 698382 sequences (100000196 bp) in 0.948 CPU sec, 0.955 real sec...
[M::process] read 696476 sequences (100000286 bp) in 1.228 CPU sec, 1.248 real sec...
[M::process] read 698862 sequences (100000060 bp) in 1.262 CPU sec, 1.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290965, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (277, 706, 1542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4072)
[M::mem_pestat] mean and std.dev: (875.36, 620.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5337)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (253.80, 122.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699312 sequences (100000259 bp) in 1.207 CPU sec, 1.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290292, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (259.86, 125.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (302, 1237, 1595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4181)
[M::mem_pestat] mean and std.dev: (1013.46, 790.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5474)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291294, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.70, 122.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (752, 809, 809)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (638, 923)
[M::mem_pestat] mean and std.dev: (805.71, 45.83)
[M::mem_pestat] low and high boundaries for proper pairs: (581, 989)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698382 reads in 221.465 CPU sec, 7.121 real sec
[M::mem_process_seqs] Processed 696476 reads in 139.986 CPU sec, 4.555 real sec
[M::mem_process_seqs] Processed 698862 reads in 151.263 CPU sec, 4.937 real sec
sorting block 0, #reads = 1092118 mem = 536879429 peak = 599924736
[M::process] read 698284 sequences (100000041 bp) in 1.732 CPU sec, 1.876 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291113, 12, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.54, 121.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 467, 815)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2177)
[M::mem_pestat] mean and std.dev: (324.60, 271.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2858)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 696548 sequences (100000286 bp) in 1.265 CPU sec, 1.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290904, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.58, 123.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699312 reads in 130.277 CPU sec, 4.135 real sec
[M::process] read 698868 sequences (100000262 bp) in 1.160 CPU sec, 1.159 real sec...
sorting block 1, #reads = 1093040 mem = 536879908 peak = 843718656
[M::mem_process_seqs] Processed 698284 reads in 81.446 CPU sec, 2.575 real sec
sorting block 2, #reads = 1093897 mem = 536878129 peak = 903729152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 289970, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (379, 768, 2247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5983)
[M::mem_pestat] mean and std.dev: (1285.67, 1297.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7851)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (259.36, 126.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 873)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698196 sequences (100000278 bp) in 1.737 CPU sec, 1.834 real sec...
[M::process] read 697282 sequences (100000160 bp) in 1.250 CPU sec, 1.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291012, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.23, 121.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696548 reads in 129.163 CPU sec, 4.114 real sec
[M::mem_process_seqs] Processed 698868 reads in 80.891 CPU sec, 2.584 real sec
sorting block 3, #reads = 1093368 mem = 536880085 peak = 903729152
[M::process] read 696018 sequences (100000089 bp) in 1.871 CPU sec, 1.994 real sec...
[M::process] read 697126 sequences (100000017 bp) in 1.230 CPU sec, 1.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290413, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.13, 121.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697282 reads in 70.995 CPU sec, 2.247 real sec
sorting block 4, #reads = 1093045 mem = 536879768 peak = 903729152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291072, 6, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (252.92, 122.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 514, 964)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2510)
[M::mem_pestat] mean and std.dev: (630.86, 592.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3283)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290065, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (256.00, 122.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696410 sequences (100000224 bp) in 1.319 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 698196 reads in 201.745 CPU sec, 6.434 real sec
[M::mem_process_seqs] Processed 696018 reads in 94.132 CPU sec, 3.018 real sec
sorting block 5, #reads = 1093634 mem = 536879229 peak = 903729152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290473, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.24, 120.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696376 sequences (100000125 bp) in 1.881 CPU sec, 2.043 real sec...
[M::process] read 694656 sequences (100000097 bp) in 1.240 CPU sec, 1.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290695, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.63, 121.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697126 reads in 131.568 CPU sec, 4.198 real sec
[M::mem_process_seqs] Processed 696410 reads in 82.420 CPU sec, 2.622 real sec
sorting block 6, #reads = 1092009 mem = 536877281 peak = 903729152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290185, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.49, 119.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696374 sequences (100000221 bp) in 2.006 CPU sec, 2.137 real sec...
[M::process] read 695488 sequences (100000288 bp) in 1.290 CPU sec, 1.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289514, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 237, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (259.62, 123.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696376 reads in 129.704 CPU sec, 4.131 real sec
[M::mem_process_seqs] Processed 694656 reads in 80.626 CPU sec, 2.565 real sec
sorting block 7, #reads = 1092891 mem = 536881931 peak = 903729152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290355, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (252.05, 118.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696716 sequences (100000090 bp) in 1.942 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290171, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (255.04, 120.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695880 sequences (100000025 bp) in 1.330 CPU sec, 1.326 real sec...
[M::mem_process_seqs] Processed 696374 reads in 130.631 CPU sec, 4.167 real sec
sorting block 8, #reads = 1091833 mem = 536878761 peak = 903729152
[M::mem_process_seqs] Processed 695488 reads in 82.017 CPU sec, 2.601 real sec
sorting block 9, #reads = 1092916 mem = 536882080 peak = 925679616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290529, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.31, 120.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697714 sequences (100000104 bp) in 2.238 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290138, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.43, 122.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698040 sequences (100000292 bp) in 1.330 CPU sec, 1.334 real sec...
[M::mem_process_seqs] Processed 696716 reads in 131.743 CPU sec, 4.193 real sec
[M::mem_process_seqs] Processed 695880 reads in 83.177 CPU sec, 2.640 real sec
sorting block 10, #reads = 1092511 mem = 536881239 peak = 925679616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290997, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.28, 121.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698374 sequences (100000298 bp) in 1.801 CPU sec, 1.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290769, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 217, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157)
[M::mem_pestat] mean and std.dev: (293.87, 155.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1481)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (253.90, 121.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698274 sequences (100000267 bp) in 1.360 CPU sec, 1.357 real sec...
[M::mem_process_seqs] Processed 697714 reads in 131.316 CPU sec, 4.199 real sec
[M::mem_process_seqs] Processed 698040 reads in 82.337 CPU sec, 2.615 real sec
sorting block 11, #reads = 1092977 mem = 536882350 peak = 925679616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291199, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.69, 123.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698146 sequences (100000040 bp) in 2.144 CPU sec, 2.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290974, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (267.22, 112.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (254.64, 123.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696534 sequences (100000022 bp) in 1.350 CPU sec, 1.355 real sec...
[M::mem_process_seqs] Processed 698374 reads in 129.988 CPU sec, 4.145 real sec
sorting block 12, #reads = 1093662 mem = 536881815 peak = 925679616
[M::mem_process_seqs] Processed 698274 reads in 81.544 CPU sec, 2.591 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291426, 7, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 287, 1847)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5123)
[M::mem_pestat] mean and std.dev: (838.40, 862.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6761)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.23, 121.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 253, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (266.70, 109.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 13, #reads = 1096258 mem = 536877960 peak = 925679616
[M::process] read 698244 sequences (100000222 bp) in 1.955 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290523, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (258.25, 124.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 869)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697714 sequences (100000161 bp) in 1.390 CPU sec, 1.395 real sec...
[M::mem_process_seqs] Processed 698146 reads in 130.004 CPU sec, 4.150 real sec
[M::mem_process_seqs] Processed 696534 reads in 81.494 CPU sec, 2.586 real sec
sorting block 14, #reads = 1094875 mem = 536878141 peak = 925679616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291250, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.39, 121.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697320 sequences (100000270 bp) in 2.252 CPU sec, 2.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291248, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.16, 122.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695118 sequences (100000123 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 698244 reads in 130.507 CPU sec, 4.159 real sec
[M::mem_process_seqs] Processed 697714 reads in 82.241 CPU sec, 2.608 real sec
sorting block 15, #reads = 1094097 mem = 536882587 peak = 925679616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290537, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.65, 122.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 443, 2522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7126)
[M::mem_pestat] mean and std.dev: (1031.80, 1146.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9428)
[M::mem_pestat] skip orientation RR
[M::process] read 696438 sequences (100000077 bp) in 2.090 CPU sec, 2.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289497, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 237, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694)
[M::mem_pestat] mean and std.dev: (260.90, 124.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 177, 792)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2088)
[M::mem_pestat] mean and std.dev: (373.73, 351.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2736)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697320 reads in 130.528 CPU sec, 4.184 real sec
[M::mem_process_seqs] Processed 695118 reads in 82.908 CPU sec, 2.628 real sec
[M::process] read 696858 sequences (100000080 bp) in 1.575 CPU sec, 1.606 real sec...
sorting block 16, #reads = 1093966 mem = 536878447 peak = 925679616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290479, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.18, 121.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697102 sequences (100000234 bp) in 1.907 CPU sec, 2.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290764, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.85, 121.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696438 reads in 131.052 CPU sec, 4.189 real sec
[M::process] read 695924 sequences (100000259 bp) in 1.370 CPU sec, 1.363 real sec...
sorting block 17, #reads = 1092279 mem = 536879051 peak = 925679616
[M::mem_process_seqs] Processed 696858 reads in 82.691 CPU sec, 2.622 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290427, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.34, 120.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 18, #reads = 1093381 mem = 536877439 peak = 982069248
[M::process] read 695834 sequences (100000127 bp) in 2.184 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290313, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (254.93, 120.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697102 reads in 131.636 CPU sec, 4.217 real sec
[M::mem_process_seqs] Processed 695924 reads in 83.348 CPU sec, 2.638 real sec
[M::process] read 696746 sequences (100000228 bp) in 1.596 CPU sec, 1.641 real sec...
sorting block 19, #reads = 1093862 mem = 536878829 peak = 982069248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289750, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 172, 1129)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3095)
[M::mem_pestat] mean and std.dev: (509.91, 465.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4078)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.28, 121.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696612 sequences (100000102 bp) in 2.028 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290821, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.65, 118.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (448, 874, 1590)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3874)
[M::mem_pestat] mean and std.dev: (1153.10, 973.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5048)
[M::mem_pestat] skip orientation RR
[M::process] read 697160 sequences (100000095 bp) in 1.350 CPU sec, 1.350 real sec...
[M::mem_process_seqs] Processed 695834 reads in 132.567 CPU sec, 4.230 real sec
[M::mem_process_seqs] Processed 696746 reads in 83.276 CPU sec, 2.643 real sec
sorting block 20, #reads = 1091796 mem = 536881259 peak = 982069248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290096, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.87, 119.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697910 sequences (100000011 bp) in 1.967 CPU sec, 2.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290502, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.14, 120.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696612 reads in 133.150 CPU sec, 4.262 real sec
[M::process] read 699296 sequences (100000215 bp) in 1.416 CPU sec, 1.429 real sec...
[M::mem_process_seqs] Processed 697160 reads in 84.577 CPU sec, 2.676 real sec
sorting block 21, #reads = 1093126 mem = 536877611 peak = 982069248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290906, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.40, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 22, #reads = 1091824 mem = 536878678 peak = 982069248
[M::process] read 699086 sequences (100000296 bp) in 2.093 CPU sec, 2.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290770, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 222, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (197.89, 80.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.20, 119.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697888 sequences (100000100 bp) in 1.420 CPU sec, 1.416 real sec...
[M::mem_process_seqs] Processed 697910 reads in 136.335 CPU sec, 4.349 real sec
[M::mem_process_seqs] Processed 699296 reads in 85.873 CPU sec, 2.726 real sec
sorting block 23, #reads = 1091166 mem = 536881871 peak = 982069248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291452, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.31, 120.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696446 sequences (100000283 bp) in 2.060 CPU sec, 2.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291483, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 430, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172)
[M::mem_pestat] mean and std.dev: (308.09, 143.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1501)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (253.87, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 302, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (305.80, 95.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 905)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699086 reads in 134.720 CPU sec, 4.300 real sec
[M::process] read 696990 sequences (100000222 bp) in 1.390 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 697888 reads in 84.546 CPU sec, 2.676 real sec
sorting block 24, #reads = 1093041 mem = 536895706 peak = 982069248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290549, 8, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 454, 932)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2498)
[M::mem_pestat] mean and std.dev: (466.18, 441.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3281)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 236, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (260.62, 125.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698584 sequences (100000271 bp) in 2.325 CPU sec, 2.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290828, 6, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 212, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (205.70, 96.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (256.27, 122.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696446 reads in 131.514 CPU sec, 4.190 real sec
[M::process] read 698334 sequences (100000282 bp) in 1.412 CPU sec, 1.419 real sec...
[M::mem_process_seqs] Processed 696990 reads in 82.683 CPU sec, 2.615 real sec
sorting block 25, #reads = 1093550 mem = 536879749 peak = 982069248
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290834, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.06, 120.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697406 sequences (100000067 bp) in 2.139 CPU sec, 2.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291188, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.30, 121.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 317, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1482)
[M::mem_pestat] mean and std.dev: (304.33, 181.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1920)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698584 reads in 132.737 CPU sec, 4.235 real sec
[M::process] read 696978 sequences (100000259 bp) in 1.433 CPU sec, 1.442 real sec...
sorting block 26, #reads = 1092648 mem = 536877909 peak = 982069248
[M::mem_process_seqs] Processed 698334 reads in 83.837 CPU sec, 2.655 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290646, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.14, 121.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1093450 mem = 536879238 peak = 1019822080
[M::process] read 695090 sequences (100000073 bp) in 2.209 CPU sec, 2.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290247, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 227, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610)
[M::mem_pestat] mean and std.dev: (189.42, 77.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 763)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (255.97, 122.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696854 sequences (100000115 bp) in 1.370 CPU sec, 1.373 real sec...
[M::mem_process_seqs] Processed 697406 reads in 131.311 CPU sec, 4.181 real sec
[M::mem_process_seqs] Processed 696978 reads in 82.666 CPU sec, 2.617 real sec
sorting block 28, #reads = 1093989 mem = 536886395 peak = 1019822080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290093, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 239, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (261.96, 125.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 321, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (279.92, 147.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RR
[M::process] read 696746 sequences (100000301 bp) in 1.931 CPU sec, 2.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290302, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 261, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (199.75, 101.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.65, 121.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696986 sequences (100000280 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_process_seqs] Processed 695090 reads in 127.550 CPU sec, 4.062 real sec
[M::mem_process_seqs] Processed 696854 reads in 81.393 CPU sec, 2.575 real sec
sorting block 29, #reads = 1092973 mem = 536877416 peak = 1019822080
[M::process] read 697004 sequences (100000300 bp) in 2.300 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290911, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.01, 120.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696986 reads in 70.337 CPU sec, 2.216 real sec
[M::process] read 697158 sequences (100000053 bp) in 1.401 CPU sec, 1.412 real sec...
sorting block 30, #reads = 1091714 mem = 536877414 peak = 1019822080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290513, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (254.03, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290823, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.39, 119.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696746 reads in 196.739 CPU sec, 6.244 real sec
[M::process] read 696812 sequences (100000189 bp) in 1.816 CPU sec, 1.839 real sec...
[M::mem_process_seqs] Processed 697004 reads in 92.938 CPU sec, 2.948 real sec
sorting block 31, #reads = 1094767 mem = 536881216 peak = 1019822080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290634, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.16, 119.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697068 sequences (100000125 bp) in 2.098 CPU sec, 2.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290504, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.95, 119.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 524, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268)
[M::mem_pestat] mean and std.dev: (313.89, 191.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1640)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697158 reads in 128.009 CPU sec, 4.086 real sec
[M::mem_process_seqs] Processed 696812 reads in 81.657 CPU sec, 2.576 real sec
[M::process] read 696960 sequences (100000257 bp) in 1.512 CPU sec, 1.552 real sec...
sorting block 32, #reads = 1093968 mem = 536881743 peak = 1019822080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290660, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.26, 120.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697836 sequences (100000008 bp) in 2.286 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290119, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 325, 712)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772)
[M::mem_pestat] mean and std.dev: (435.92, 443.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2302)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.54, 119.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697068 reads in 129.584 CPU sec, 4.122 real sec
sorting block 33, #reads = 1092774 mem = 536879169 peak = 1019822080
[M::process] read 696282 sequences (100000006 bp) in 1.414 CPU sec, 1.436 real sec...
[M::mem_process_seqs] Processed 696960 reads in 83.203 CPU sec, 2.628 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290188, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 34, #reads = 1091980 mem = 536885181 peak = 1058852864
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.91, 119.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696324 sequences (100000060 bp) in 1.922 CPU sec, 2.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290055, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 862, 862)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144)
[M::mem_pestat] mean and std.dev: (609.00, 346.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2785)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (256.64, 120.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697836 reads in 128.487 CPU sec, 4.095 real sec
[M::process] read 697238 sequences (100000008 bp) in 1.369 CPU sec, 1.362 real sec...
[M::mem_process_seqs] Processed 696282 reads in 81.499 CPU sec, 2.572 real sec
sorting block 35, #reads = 1091791 mem = 536881770 peak = 1058852864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289992, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 555, 2721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7767)
[M::mem_pestat] mean and std.dev: (1347.36, 1408.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10290)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.05, 121.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 694868 sequences (100000009 bp) in 2.213 CPU sec, 2.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290971, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.54, 119.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696324 reads in 126.367 CPU sec, 4.013 real sec
[M::process] read 697744 sequences (100000001 bp) in 1.340 CPU sec, 1.343 real sec...
[M::mem_process_seqs] Processed 697238 reads in 80.100 CPU sec, 2.532 real sec
sorting block 36, #reads = 1093397 mem = 536879092 peak = 1058852864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289627, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 241, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (264.02, 126.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698928 sequences (100000118 bp) in 1.965 CPU sec, 2.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291065, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.68, 122.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694868 reads in 126.109 CPU sec, 4.021 real sec
[M::process] read 698304 sequences (100000059 bp) in 1.427 CPU sec, 1.442 real sec...
sorting block 37, #reads = 1093443 mem = 536880318 peak = 1058852864
[M::mem_process_seqs] Processed 697744 reads in 80.697 CPU sec, 2.550 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291285, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 172, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (189.40, 64.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 640)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.48, 121.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 38, #reads = 1092706 mem = 536881396 peak = 1058852864
[M::process] read 698042 sequences (100000158 bp) in 2.225 CPU sec, 2.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291201, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.41, 120.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698928 reads in 127.706 CPU sec, 4.062 real sec
[M::process] read 696904 sequences (100000092 bp) in 1.474 CPU sec, 1.486 real sec...
[M::mem_process_seqs] Processed 698304 reads in 81.011 CPU sec, 2.556 real sec
sorting block 39, #reads = 1094400 mem = 536877841 peak = 1058852864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290370, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.17, 120.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696728 sequences (100000188 bp) in 1.967 CPU sec, 2.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290232, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 298, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (258.55, 73.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (257.10, 123.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698042 reads in 127.877 CPU sec, 4.076 real sec
[M::process] read 697366 sequences (100000128 bp) in 1.394 CPU sec, 1.400 real sec...
[M::mem_process_seqs] Processed 696904 reads in 81.264 CPU sec, 2.569 real sec
sorting block 40, #reads = 1094119 mem = 536879742 peak = 1058852864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290085, 8, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 362, 2973)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8495)
[M::mem_pestat] mean and std.dev: (1292.17, 1492.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11256)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (257.16, 123.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 177, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 303)
[M::mem_pestat] mean and std.dev: (171.89, 14.82)
[M::mem_pestat] low and high boundaries for proper pairs: (15, 351)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 696140 sequences (100000102 bp) in 2.409 CPU sec, 2.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290787, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (278, 324, 2299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6341)
[M::mem_pestat] mean and std.dev: (772.58, 884.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8362)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.02, 121.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 176, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 400)
[M::mem_pestat] mean and std.dev: (208.45, 66.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696728 reads in 127.687 CPU sec, 4.061 real sec
[M::mem_process_seqs] Processed 697366 reads in 81.750 CPU sec, 2.580 real sec
[M::process] read 696576 sequences (100000274 bp) in 1.530 CPU sec, 1.564 real sec...
sorting block 41, #reads = 1092977 mem = 536879770 peak = 1058852864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290328, 8, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 345, 1723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4867)
[M::mem_pestat] mean and std.dev: (571.67, 564.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6439)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (258.40, 123.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697226 sequences (100000166 bp) in 2.069 CPU sec, 2.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 289857, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.87, 120.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696140 reads in 128.853 CPU sec, 4.114 real sec
sorting block 42, #reads = 1093476 mem = 536883401 peak = 1058852864
[M::mem_process_seqs] Processed 696576 reads in 82.706 CPU sec, 2.611 real sec
[M::process] read 696990 sequences (100000223 bp) in 1.636 CPU sec, 1.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290390, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.36, 118.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 175, 1897)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5441)
[M::mem_pestat] mean and std.dev: (832.91, 927.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7213)
[M::mem_pestat] skip orientation RR
sorting block 43, #reads = 1092909 mem = 536882321 peak = 1067155456
[M::process] read 697136 sequences (100000177 bp) in 2.214 CPU sec, 2.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290580, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.25, 119.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 264, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (230.67, 80.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 617)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697226 reads in 131.318 CPU sec, 4.182 real sec
[M::mem_process_seqs] Processed 696990 reads in 83.971 CPU sec, 2.648 real sec
[M::process] read 696624 sequences (100000112 bp) in 1.534 CPU sec, 1.557 real sec...
sorting block 44, #reads = 1092465 mem = 536878912 peak = 1067155456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290343, 5, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 272, 1000)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2564)
[M::mem_pestat] mean and std.dev: (514.08, 360.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3346)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.98, 119.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700254 sequences (100000021 bp) in 2.100 CPU sec, 2.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290136, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.20, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697136 reads in 130.881 CPU sec, 4.173 real sec
[M::mem_process_seqs] Processed 696624 reads in 83.702 CPU sec, 2.640 real sec
[M::process] read 701834 sequences (100000183 bp) in 1.581 CPU sec, 1.618 real sec...
sorting block 45, #reads = 1091109 mem = 536880290 peak = 1067155456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291319, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.32, 117.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701704 sequences (100000003 bp) in 2.241 CPU sec, 2.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 292409, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.32, 120.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702658 sequences (100000131 bp) in 1.370 CPU sec, 1.372 real sec...
[M::mem_process_seqs] Processed 700254 reads in 137.203 CPU sec, 4.363 real sec
sorting block 46, #reads = 1092184 mem = 536879744 peak = 1067155456
[M::mem_process_seqs] Processed 701834 reads in 86.956 CPU sec, 2.754 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292219, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 201, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (215.36, 75.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (244.14, 119.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 47, #reads = 1091943 mem = 536877138 peak = 1067155456
[M::process] read 702640 sequences (100000230 bp) in 2.135 CPU sec, 2.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 292320, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 199, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393)
[M::mem_pestat] mean and std.dev: (169.87, 47.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 481)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.10, 117.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 433, 1218)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3204)
[M::mem_pestat] mean and std.dev: (639.69, 571.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4197)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701704 reads in 135.226 CPU sec, 4.312 real sec
[M::process] read 699440 sequences (100000247 bp) in 1.548 CPU sec, 1.566 real sec...
[M::mem_process_seqs] Processed 702658 reads in 86.498 CPU sec, 2.729 real sec
sorting block 48, #reads = 1093253 mem = 536878377 peak = 1067155456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292523, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.41, 116.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702742 sequences (100000224 bp) in 2.201 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292080, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (251.57, 123.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701344 sequences (100000135 bp) in 1.410 CPU sec, 1.407 real sec...
[M::mem_process_seqs] Processed 702640 reads in 134.154 CPU sec, 4.262 real sec
[M::mem_process_seqs] Processed 699440 reads in 84.477 CPU sec, 2.672 real sec
sorting block 49, #reads = 1094533 mem = 536879304 peak = 1067155456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292374, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.33, 116.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698554 sequences (100000119 bp) in 2.153 CPU sec, 2.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292048, 9, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (273, 348, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1188)
[M::mem_pestat] mean and std.dev: (343.44, 143.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1493)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.70, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702742 reads in 137.695 CPU sec, 4.391 real sec
[M::process] read 698420 sequences (100000230 bp) in 1.487 CPU sec, 1.500 real sec...
[M::mem_process_seqs] Processed 701344 reads in 86.939 CPU sec, 2.747 real sec
sorting block 50, #reads = 1093668 mem = 536878409 peak = 1067155456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291321, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (281, 619, 936)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2246)
[M::mem_pestat] mean and std.dev: (506.50, 315.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2901)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (253.10, 124.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698232 sequences (100000080 bp) in 2.338 CPU sec, 2.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290836, 14, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (253.25, 123.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (96, 628, 869)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2415)
[M::mem_pestat] mean and std.dev: (555.64, 458.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3188)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (189.30, 56.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698554 reads in 135.903 CPU sec, 4.321 real sec
[M::process] read 697538 sequences (100000223 bp) in 1.419 CPU sec, 1.419 real sec...
sorting block 51, #reads = 1093664 mem = 536878949 peak = 1067155456
[M::mem_process_seqs] Processed 698420 reads in 86.309 CPU sec, 2.732 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290658, 3, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 224, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491)
[M::mem_pestat] mean and std.dev: (179.88, 51.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (253.03, 123.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 52, #reads = 1092117 mem = 536878569 peak = 1076551680
[M::process] read 697124 sequences (100000180 bp) in 2.029 CPU sec, 2.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290603, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.44, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698232 reads in 135.932 CPU sec, 4.334 real sec
[M::process] read 697216 sequences (100000035 bp) in 1.475 CPU sec, 1.486 real sec...
[M::mem_process_seqs] Processed 697538 reads in 86.245 CPU sec, 2.726 real sec
sorting block 53, #reads = 1091772 mem = 536878904 peak = 1076551680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290691, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 584, 584)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486)
[M::mem_pestat] mean and std.dev: (373.78, 294.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1937)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.69, 123.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 280, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508)
[M::mem_pestat] mean and std.dev: (241.54, 72.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 622)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697206 sequences (100000030 bp) in 2.262 CPU sec, 2.377 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290646, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.93, 121.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697300 sequences (100000235 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_process_seqs] Processed 697124 reads in 136.016 CPU sec, 4.321 real sec
[M::mem_process_seqs] Processed 697216 reads in 86.009 CPU sec, 2.722 real sec
sorting block 54, #reads = 1090745 mem = 536879544 peak = 1076551680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290188, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.38, 121.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697496 sequences (100000096 bp) in 1.969 CPU sec, 2.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290989, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.80, 120.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 177, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (194.00, 88.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RR
[M::process] read 703224 sequences (100000182 bp) in 1.430 CPU sec, 1.438 real sec...
[M::mem_process_seqs] Processed 697206 reads in 135.879 CPU sec, 4.329 real sec
sorting block 55, #reads = 1089887 mem = 536879295 peak = 1076551680
[M::mem_process_seqs] Processed 697300 reads in 85.772 CPU sec, 2.714 real sec
sorting block 56, #reads = 1089176 mem = 536879262 peak = 1089912832
[M::process] read 704764 sequences (100000260 bp) in 2.320 CPU sec, 2.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292358, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (240.21, 116.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703656 sequences (100000264 bp) in 1.450 CPU sec, 1.449 real sec...
[M::mem_process_seqs] Processed 703224 reads in 82.224 CPU sec, 2.590 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290809, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.92, 121.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292987, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 312, 857)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2275)
[M::mem_pestat] mean and std.dev: (419.83, 398.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2984)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (238.13, 115.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702894 sequences (100000081 bp) in 1.599 CPU sec, 1.608 real sec...
[M::mem_process_seqs] Processed 697496 reads in 225.512 CPU sec, 7.158 real sec
sorting block 57, #reads = 1088455 mem = 536877854 peak = 1089912832
[M::mem_process_seqs] Processed 704764 reads in 110.370 CPU sec, 3.520 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292527, 4, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (278, 318, 1854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5006)
[M::mem_pestat] mean and std.dev: (779.67, 777.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6582)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.34, 115.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 262, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (280.09, 139.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1367)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 58, #reads = 1088390 mem = 536880876 peak = 1089912832
[M::process] read 701400 sequences (100000090 bp) in 2.101 CPU sec, 2.236 real sec...
[M::process] read 702238 sequences (100000171 bp) in 1.410 CPU sec, 1.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292466, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.85, 115.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703656 reads in 147.178 CPU sec, 4.684 real sec
[M::mem_process_seqs] Processed 702894 reads in 92.376 CPU sec, 2.935 real sec
sorting block 59, #reads = 1088927 mem = 536876257 peak = 1089912832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292600, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.28, 117.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702640 sequences (100000263 bp) in 2.225 CPU sec, 2.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292748, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (240.96, 115.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701148 sequences (100000192 bp) in 1.430 CPU sec, 1.431 real sec...
[M::mem_process_seqs] Processed 701400 reads in 142.924 CPU sec, 4.544 real sec
[M::mem_process_seqs] Processed 702238 reads in 89.190 CPU sec, 2.825 real sec
sorting block 60, #reads = 1090452 mem = 536878743 peak = 1089912832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292966, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.07, 116.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698980 sequences (100000289 bp) in 1.921 CPU sec, 2.026 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292141, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.56, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699480 sequences (100000201 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 702640 reads in 141.788 CPU sec, 4.522 real sec
[M::mem_process_seqs] Processed 701148 reads in 90.217 CPU sec, 2.865 real sec
sorting block 61, #reads = 1092795 mem = 536878533 peak = 1089912832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291004, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (253.59, 124.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (341, 2930, 3974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11240)
[M::mem_pestat] mean and std.dev: (2137.70, 1525.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14873)
[M::mem_pestat] skip orientation RR
[M::process] read 698368 sequences (100000085 bp) in 2.375 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291540, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 282, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1777)
[M::mem_pestat] mean and std.dev: (550.55, 558.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2784)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.96, 121.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 384, 1923)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5443)
[M::mem_pestat] mean and std.dev: (763.00, 779.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7203)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697768 sequences (100000019 bp) in 1.400 CPU sec, 1.402 real sec...
[M::mem_process_seqs] Processed 698980 reads in 139.099 CPU sec, 4.423 real sec
sorting block 62, #reads = 1090987 mem = 536878838 peak = 1089912832
[M::mem_process_seqs] Processed 699480 reads in 87.469 CPU sec, 2.772 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290960, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.89, 122.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 157, 195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 295)
[M::mem_pestat] mean and std.dev: (180.20, 58.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 413)
[M::mem_pestat] skip orientation RR
sorting block 63, #reads = 1090532 mem = 536876244 peak = 1118937088
[M::process] read 697136 sequences (100000085 bp) in 2.130 CPU sec, 2.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290486, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.45, 123.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 310, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (348.00, 142.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698368 reads in 138.584 CPU sec, 4.420 real sec
[M::process] read 698268 sequences (100000123 bp) in 1.480 CPU sec, 1.492 real sec...
[M::mem_process_seqs] Processed 697768 reads in 88.197 CPU sec, 2.783 real sec
sorting block 64, #reads = 1090890 mem = 536884219 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290847, 5, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (374, 560, 1053)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2411)
[M::mem_pestat] mean and std.dev: (605.50, 331.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3090)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.52, 124.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698456 sequences (100000270 bp) in 2.211 CPU sec, 2.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291238, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.44, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698676 sequences (100000006 bp) in 1.410 CPU sec, 1.413 real sec...
[M::mem_process_seqs] Processed 697136 reads in 137.022 CPU sec, 4.357 real sec
[M::mem_process_seqs] Processed 698268 reads in 87.177 CPU sec, 2.758 real sec
sorting block 65, #reads = 1090672 mem = 536877773 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290775, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.57, 121.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 304, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (302.11, 178.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1338)
[M::mem_pestat] skip orientation RR
[M::process] read 701870 sequences (100000256 bp) in 2.055 CPU sec, 2.157 real sec...
[M::process] read 702982 sequences (100000086 bp) in 1.360 CPU sec, 1.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290844, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.57, 121.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 212, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (252.75, 164.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698456 reads in 141.401 CPU sec, 4.501 real sec
sorting block 66, #reads = 1091470 mem = 536882266 peak = 1118937088
[M::mem_process_seqs] Processed 698676 reads in 91.059 CPU sec, 2.889 real sec
sorting block 67, #reads = 1089066 mem = 536880212 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291865, 7, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 233, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 369)
[M::mem_pestat] mean and std.dev: (184.50, 56.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 436)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.28, 117.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (127, 203, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469)
[M::mem_pestat] mean and std.dev: (187.90, 75.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 583)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 701378 sequences (100000141 bp) in 2.264 CPU sec, 2.365 real sec...
[M::process] read 701418 sequences (100000207 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292825, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (242.22, 117.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701870 reads in 146.468 CPU sec, 4.656 real sec
[M::mem_process_seqs] Processed 702982 reads in 87.880 CPU sec, 2.791 real sec
sorting block 68, #reads = 1087119 mem = 536881703 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291850, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.04, 118.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698138 sequences (100000045 bp) in 2.039 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292423, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (246.44, 120.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 136, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (227.82, 130.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RR
[M::process] read 701708 sequences (100000184 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_process_seqs] Processed 701378 reads in 133.910 CPU sec, 4.264 real sec
[M::mem_process_seqs] Processed 701418 reads in 84.418 CPU sec, 2.668 real sec
sorting block 69, #reads = 1094568 mem = 536879946 peak = 1118937088
[M::process] read 702110 sequences (100000081 bp) in 2.319 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292267, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.66, 116.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700976 sequences (100000101 bp) in 1.380 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 701708 reads in 78.430 CPU sec, 2.473 real sec
sorting block 70, #reads = 1093926 mem = 536879440 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291252, 7, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 191, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 362)
[M::mem_pestat] mean and std.dev: (189.00, 35.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (255.30, 123.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292038, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.68, 115.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698138 reads in 208.091 CPU sec, 6.617 real sec
[M::process] read 700770 sequences (100000097 bp) in 1.568 CPU sec, 1.580 real sec...
[M::mem_process_seqs] Processed 702110 reads in 99.570 CPU sec, 3.170 real sec
sorting block 71, #reads = 1092750 mem = 536878772 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291703, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.83, 116.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698266 sequences (100000172 bp) in 2.334 CPU sec, 2.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291936, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.60, 118.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700976 reads in 134.379 CPU sec, 4.289 real sec
[M::mem_process_seqs] Processed 700770 reads in 85.611 CPU sec, 2.704 real sec
[M::process] read 700256 sequences (100000088 bp) in 1.671 CPU sec, 1.741 real sec...
sorting block 72, #reads = 1092466 mem = 536880624 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291642, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (254.42, 123.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699964 sequences (100000042 bp) in 1.952 CPU sec, 2.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292019, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.70, 118.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698266 reads in 132.961 CPU sec, 4.245 real sec
[M::mem_process_seqs] Processed 700256 reads in 84.279 CPU sec, 2.660 real sec
sorting block 73, #reads = 1092976 mem = 536879062 peak = 1118937088
[M::process] read 699322 sequences (100000148 bp) in 1.547 CPU sec, 1.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291307, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.41, 119.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1093066 mem = 536876241 peak = 1118937088
[M::process] read 698982 sequences (100000137 bp) in 1.958 CPU sec, 2.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291317, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.05, 121.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699964 reads in 134.549 CPU sec, 4.296 real sec
[M::process] read 699168 sequences (100000111 bp) in 1.478 CPU sec, 1.493 real sec...
[M::mem_process_seqs] Processed 699322 reads in 85.837 CPU sec, 2.710 real sec
sorting block 75, #reads = 1093500 mem = 536881870 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291007, 11, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 335, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (298.00, 62.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.56, 120.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (659, 743, 2319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5639)
[M::mem_pestat] mean and std.dev: (1101.91, 903.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7299)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 699226 sequences (100000216 bp) in 2.168 CPU sec, 2.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291027, 9, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.18, 120.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 406, 876)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2252)
[M::mem_pestat] mean and std.dev: (474.00, 315.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2940)
[M::mem_pestat] skip orientation RR
[M::process] read 699588 sequences (100000119 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_process_seqs] Processed 698982 reads in 136.982 CPU sec, 4.354 real sec
[M::mem_process_seqs] Processed 699168 reads in 87.162 CPU sec, 2.763 real sec
sorting block 76, #reads = 1091429 mem = 536880882 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291069, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.50, 120.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703916 sequences (100000123 bp) in 2.062 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291181, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (245.05, 117.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704294 sequences (100000287 bp) in 1.450 CPU sec, 1.444 real sec...
[M::mem_process_seqs] Processed 699226 reads in 140.445 CPU sec, 4.470 real sec
sorting block 77, #reads = 1090432 mem = 536886141 peak = 1118937088
[M::mem_process_seqs] Processed 699588 reads in 90.697 CPU sec, 2.871 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292050, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (237.92, 114.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1089466 mem = 536879105 peak = 1118937088
[M::process] read 704656 sequences (100000106 bp) in 2.448 CPU sec, 2.593 real sec...
[M::process] read 701952 sequences (100000056 bp) in 1.450 CPU sec, 1.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292688, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (240.89, 117.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703916 reads in 156.236 CPU sec, 4.964 real sec
[M::mem_process_seqs] Processed 704294 reads in 98.191 CPU sec, 3.114 real sec
sorting block 79, #reads = 1085687 mem = 536880330 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293482, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (237.24, 114.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703650 sequences (100000224 bp) in 2.132 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292583, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 220, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (247.03, 120.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704734 sequences (100000204 bp) in 1.430 CPU sec, 1.424 real sec...
[M::mem_process_seqs] Processed 704656 reads in 147.937 CPU sec, 4.709 real sec
[M::mem_process_seqs] Processed 701952 reads in 92.365 CPU sec, 2.931 real sec
sorting block 80, #reads = 1088044 mem = 536877746 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292747, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (240.54, 116.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703152 sequences (100000115 bp) in 2.169 CPU sec, 2.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292868, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 210, 651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1659)
[M::mem_pestat] mean and std.dev: (299.55, 231.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2163)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (235.74, 113.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701748 sequences (100000176 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 703650 reads in 142.855 CPU sec, 4.547 real sec
[M::mem_process_seqs] Processed 704734 reads in 90.586 CPU sec, 2.870 real sec
sorting block 81, #reads = 1089125 mem = 536879908 peak = 1118937088
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292855, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.31, 113.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 353, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 559)
[M::mem_pestat] mean and std.dev: (301.62, 73.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 658)
[M::mem_pestat] skip orientation RR
[M::process] read 699682 sequences (100000131 bp) in 2.007 CPU sec, 2.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292048, 8, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.21, 116.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 301, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149)
[M::mem_pestat] mean and std.dev: (348.15, 213.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1473)
[M::mem_pestat] skip orientation RR
[M::process] read 701118 sequences (100000203 bp) in 1.390 CPU sec, 1.389 real sec...
[M::mem_process_seqs] Processed 703152 reads in 141.786 CPU sec, 4.518 real sec
sorting block 82, #reads = 1092006 mem = 536887209 peak = 1118937088
[M::mem_process_seqs] Processed 701748 reads in 88.807 CPU sec, 2.816 real sec
sorting block 83, #reads = 1091639 mem = 536878164 peak = 1119367168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291621, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (322, 322, 695)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441)
[M::mem_pestat] mean and std.dev: (425.82, 250.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1814)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.07, 119.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700010 sequences (100000195 bp) in 2.060 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291916, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.49, 117.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (466, 963, 1340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3088)
[M::mem_pestat] mean and std.dev: (888.18, 582.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3962)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 699970 sequences (100000228 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 699682 reads in 139.012 CPU sec, 4.429 real sec
[M::mem_process_seqs] Processed 701118 reads in 87.594 CPU sec, 2.773 real sec
sorting block 84, #reads = 1090234 mem = 536878377 peak = 1119367168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291509, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.15, 118.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699220 sequences (100000039 bp) in 2.162 CPU sec, 2.314 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292236, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.77, 118.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 250, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (259.70, 90.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation RR
[M::process] read 699048 sequences (100000295 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 700010 reads in 137.678 CPU sec, 4.380 real sec
[M::mem_process_seqs] Processed 699970 reads in 86.765 CPU sec, 2.751 real sec
sorting block 85, #reads = 1092710 mem = 536884192 peak = 1119367168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290906, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.11, 119.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698950 sequences (100000234 bp) in 2.104 CPU sec, 2.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291448, 8, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.75, 119.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (480, 480, 828)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524)
[M::mem_pestat] mean and std.dev: (524.70, 357.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1954)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699220 reads in 136.905 CPU sec, 4.361 real sec
[M::process] read 698596 sequences (100000063 bp) in 1.390 CPU sec, 1.394 real sec...
sorting block 86, #reads = 1092613 mem = 536880844 peak = 1119367168
[M::mem_process_seqs] Processed 699048 reads in 86.629 CPU sec, 2.738 real sec
[M::process] read 251102 sequences (35839694 bp) in 1.004 CPU sec, 1.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291024, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.83, 119.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (292, 1093, 1093)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2695)
[M::mem_pestat] mean and std.dev: (604.12, 394.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3496)
[M::mem_pestat] skip orientation RR
sorting block 87, #reads = 1090994 mem = 536883075 peak = 1119367168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290943, 11, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.62, 120.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (9, 97, 649)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1929)
[M::mem_pestat] mean and std.dev: (273.40, 321.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2569)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698950 reads in 133.856 CPU sec, 4.339 real sec
[M::mem_process_seqs] Processed 698596 reads in 84.190 CPU sec, 2.728 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 104221, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (243.43, 117.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 251102 reads in 28.728 CPU sec, 0.971 real sec
sorting block 88, #reads = 1091165 mem = 536877611 peak = 1119367168
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 97374976
overall: 70037262336 mem 11548.367 user 361.498 sys 1230.095 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1230.129 sec; CPU: 11909.867 sec
sorting block 89, #reads = 668494 mem = 329695583 peak = 1130205184
uniq header count 1
merging 90 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5231758078
output reads: 97872164
bam_mem_sort: 90 calls 31.278 user 0.172 sys 31.647 real
bam_write: 90 calls 62.312 user 1.181 sys 64.109 real
execute: 1 calls 18.510 user 1.107 sys 1257.817 real
merge_files: 1 calls 18.489 user 1.088 sys 26.818 real
parse_chunk: 1434 calls 44.044 user 13.348 sys 58.812 real
read_chunk: 3873 calls 36.169 user 35.108 sys 339.762 real
sort_block: 1 calls 28.958 user 34.570 sys 328.385 real
write_chunk: 2437 calls 18.110 user 0.740 sys 25.888 real
overall: 3019874304 mem 1041.186 user 58.296 sys 1257.837 real
ls: cannot access '*.cram': No such file or directory