cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_IWL-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1916.253 CPU sec, 67.204 real sec
[M::process] read 768240 sequences (100000075 bp) in 1.554 CPU sec, 1.561 real sec...
[M::process] read 767970 sequences (100000264 bp) in 1.789 CPU sec, 1.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 303464, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (107, 283, 780)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2126)
[M::mem_pestat] mean and std.dev: (292.33, 272.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2799)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.88, 66.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 160, 634)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1678)
[M::mem_pestat] mean and std.dev: (272.21, 252.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2200)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 767988 sequences (100000170 bp) in 1.828 CPU sec, 1.834 real sec...
[M::process] read 768692 sequences (100000089 bp) in 1.740 CPU sec, 1.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 303850, 10, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 323, 851)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2273)
[M::mem_pestat] mean and std.dev: (423.44, 372.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2984)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.25, 66.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (670, 4376, 4489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12127)
[M::mem_pestat] mean and std.dev: (3122.80, 2424.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15946)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (289, 796, 3356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9490)
[M::mem_pestat] mean and std.dev: (1573.29, 1844.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12557)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 768240 reads in 219.691 CPU sec, 6.920 real sec
[M::mem_process_seqs] Processed 767970 reads in 151.276 CPU sec, 4.811 real sec
sorting block 0, #reads = 1110735 mem = 536880204 peak = 606527488
[M::process] read 766638 sequences (100000097 bp) in 2.784 CPU sec, 2.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 304569, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (109, 148, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (257.08, 232.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1373)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.40, 66.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 169, 859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2297)
[M::mem_pestat] mean and std.dev: (227.43, 187.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3016)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 765378 sequences (100000007 bp) in 2.090 CPU sec, 2.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 303803, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 256, 1186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3306)
[M::mem_pestat] mean and std.dev: (649.67, 791.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4366)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.26, 66.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 768692 reads in 245.156 CPU sec, 7.909 real sec
sorting block 1, #reads = 1113139 mem = 536880750 peak = 792125440
[M::mem_process_seqs] Processed 767988 reads in 288.047 CPU sec, 9.321 real sec
[M::process] read 763936 sequences (100000157 bp) in 2.751 CPU sec, 2.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 303490, 7, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 344, 920)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2356)
[M::mem_pestat] mean and std.dev: (552.85, 608.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3074)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (162.01, 67.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 267, 910)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2374)
[M::mem_pestat] mean and std.dev: (379.50, 299.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3106)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 764332 sequences (100000069 bp) in 2.114 CPU sec, 2.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 303716, 12, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.33, 68.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (523, 1176, 2967)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7855)
[M::mem_pestat] mean and std.dev: (1245.09, 897.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10299)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 243, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171)
[M::mem_pestat] mean and std.dev: (291.15, 198.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1500)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 302973, 7, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 1023, 2253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6401)
[M::mem_pestat] mean and std.dev: (897.00, 733.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8475)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (163.99, 68.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 578, 814)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2162)
[M::mem_pestat] mean and std.dev: (455.09, 356.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2836)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 766638 reads in 355.585 CPU sec, 11.937 real sec
sorting block 2, #reads = 1111914 mem = 536885423 peak = 872976384
[M::mem_process_seqs] Processed 765378 reads in 287.490 CPU sec, 9.837 real sec
[M::mem_process_seqs] Processed 763936 reads in 314.522 CPU sec, 10.802 real sec
sorting block 3, #reads = 1111648 mem = 536877961 peak = 872976384
[M::process] read 761794 sequences (100000199 bp) in 2.762 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 302756, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 168, 1133)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3121)
[M::mem_pestat] mean and std.dev: (656.93, 792.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4115)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.62, 68.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 917, 1395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3631)
[M::mem_pestat] mean and std.dev: (855.69, 630.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4749)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 760932 sequences (100000193 bp) in 2.736 CPU sec, 3.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 301854, 12, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 221, 1694)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4820)
[M::mem_pestat] mean and std.dev: (694.58, 778.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6383)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (165.75, 70.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 1340, 1835)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5135)
[M::mem_pestat] mean and std.dev: (1085.00, 924.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6785)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 364, 2129)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6033)
[M::mem_pestat] mean and std.dev: (1299.35, 1730.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8223)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 760690 sequences (100000022 bp) in 2.149 CPU sec, 2.160 real sec...
[M::mem_process_seqs] Processed 764332 reads in 261.002 CPU sec, 8.581 real sec
sorting block 4, #reads = 1110622 mem = 536876896 peak = 872976384
[M::mem_process_seqs] Processed 761794 reads in 181.164 CPU sec, 5.950 real sec
sorting block 5, #reads = 1108682 mem = 536878552 peak = 879611904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 301477, 10, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 392, 631)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1579)
[M::mem_pestat] mean and std.dev: (388.00, 244.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2053)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 153, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (166.48, 70.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (563, 1263, 1534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3476)
[M::mem_pestat] mean and std.dev: (961.33, 561.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4447)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (281, 422, 819)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1895)
[M::mem_pestat] mean and std.dev: (521.18, 402.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2433)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 760572 sequences (100000233 bp) in 2.924 CPU sec, 3.118 real sec...
[M::process] read 760442 sequences (100000022 bp) in 2.338 CPU sec, 2.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 301546, 12, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 460, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3027)
[M::mem_pestat] mean and std.dev: (675.89, 740.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3980)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 153, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (166.48, 70.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (368, 368, 949)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2111)
[M::mem_pestat] mean and std.dev: (531.73, 480.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2692)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 470, 1349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3637)
[M::mem_pestat] mean and std.dev: (641.71, 587.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4781)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 760932 reads in 249.455 CPU sec, 8.152 real sec
[M::mem_process_seqs] Processed 760690 reads in 174.524 CPU sec, 5.684 real sec
sorting block 6, #reads = 1108029 mem = 536879076 peak = 879611904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 301439, 12, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 187, 863)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2327)
[M::mem_pestat] mean and std.dev: (343.08, 285.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3059)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 153, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (166.33, 69.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (307, 1254, 2235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6091)
[M::mem_pestat] mean and std.dev: (1222.73, 1323.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8019)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 153, 721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937)
[M::mem_pestat] mean and std.dev: (344.37, 343.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2545)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 759236 sequences (100000094 bp) in 2.891 CPU sec, 3.104 real sec...
[M::process] read 763058 sequences (100000172 bp) in 2.231 CPU sec, 2.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 300920, 17, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 570, 1577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4371)
[M::mem_pestat] mean and std.dev: (524.89, 450.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5768)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 153, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (166.59, 70.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (415, 504, 2354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6232)
[M::mem_pestat] mean and std.dev: (1123.35, 1182.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8171)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 470, 2063)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5787)
[M::mem_pestat] mean and std.dev: (1078.39, 1511.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7649)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 760572 reads in 242.546 CPU sec, 7.841 real sec
sorting block 7, #reads = 1107641 mem = 536881065 peak = 902414336
[M::mem_process_seqs] Processed 760442 reads in 169.974 CPU sec, 5.500 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 301573, 12, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 207, 825)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2197)
[M::mem_pestat] mean and std.dev: (516.41, 512.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2883)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 154, 210)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402)
[M::mem_pestat] mean and std.dev: (167.44, 70.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 498)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (628, 628, 1380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2884)
[M::mem_pestat] mean and std.dev: (816.00, 508.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3636)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 219, 1125)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3097)
[M::mem_pestat] mean and std.dev: (559.00, 706.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4083)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 768394 sequences (100000243 bp) in 3.053 CPU sec, 3.261 real sec...
sorting block 8, #reads = 1108251 mem = 536884527 peak = 902414336
[M::process] read 768756 sequences (100000043 bp) in 2.481 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 302009, 13, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 211, 1176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3280)
[M::mem_pestat] mean and std.dev: (711.00, 713.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4332)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (164.48, 69.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 453, 1400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3994)
[M::mem_pestat] mean and std.dev: (644.75, 788.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5291)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 222, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1976)
[M::mem_pestat] mean and std.dev: (339.82, 266.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2580)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 759236 reads in 245.775 CPU sec, 7.919 real sec
[M::mem_process_seqs] Processed 763058 reads in 174.601 CPU sec, 5.611 real sec
sorting block 9, #reads = 1107384 mem = 536879803 peak = 902414336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 303820, 9, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 217, 1280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3556)
[M::mem_pestat] mean and std.dev: (711.53, 854.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4694)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.84, 66.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 188, 871)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2389)
[M::mem_pestat] mean and std.dev: (283.82, 240.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3148)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 767182 sequences (100000030 bp) in 3.143 CPU sec, 3.376 real sec...
[M::process] read 767816 sequences (100000029 bp) in 2.274 CPU sec, 2.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304252, 14, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 518, 1243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3321)
[M::mem_pestat] mean and std.dev: (679.93, 593.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4360)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.41, 66.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (265, 738, 1340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3490)
[M::mem_pestat] mean and std.dev: (889.71, 611.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4565)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 471, 724)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832)
[M::mem_pestat] mean and std.dev: (367.57, 222.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2386)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 768394 reads in 246.924 CPU sec, 7.960 real sec
[M::mem_process_seqs] Processed 768756 reads in 173.920 CPU sec, 5.601 real sec
sorting block 10, #reads = 1111080 mem = 536885970 peak = 902414336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 304123, 9, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (324, 332, 2601)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7155)
[M::mem_pestat] mean and std.dev: (849.00, 946.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9432)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.48, 67.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 213, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (202.33, 109.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 766286 sequences (100000195 bp) in 3.139 CPU sec, 3.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 304147, 5, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 156, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231)
[M::mem_pestat] mean and std.dev: (226.00, 165.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1601)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.26, 67.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 235, 1240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3428)
[M::mem_pestat] mean and std.dev: (586.69, 584.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4522)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 765212 sequences (100000258 bp) in 2.746 CPU sec, 2.857 real sec...
[M::mem_process_seqs] Processed 767182 reads in 243.743 CPU sec, 7.838 real sec
sorting block 11, #reads = 1114058 mem = 536886550 peak = 902414336
[M::mem_process_seqs] Processed 767816 reads in 170.852 CPU sec, 5.494 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 303696, 9, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 592, 1576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4348)
[M::mem_pestat] mean and std.dev: (882.00, 1106.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5734)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (162.59, 68.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 224, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260)
[M::mem_pestat] mean and std.dev: (252.88, 146.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1636)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 12, #reads = 1112461 mem = 536883813 peak = 902414336
[M::process] read 764494 sequences (100000218 bp) in 3.645 CPU sec, 4.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 303355, 11, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 391, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150)
[M::mem_pestat] mean and std.dev: (301.67, 174.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.27, 68.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (524, 621, 1879)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4589)
[M::mem_pestat] mean and std.dev: (1288.36, 1269.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6365)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 372, 1322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3666)
[M::mem_pestat] mean and std.dev: (502.08, 491.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4838)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 763096 sequences (100000035 bp) in 2.321 CPU sec, 2.366 real sec...
[M::mem_process_seqs] Processed 766286 reads in 241.694 CPU sec, 7.770 real sec
[M::mem_process_seqs] Processed 765212 reads in 171.466 CPU sec, 5.524 real sec
sorting block 13, #reads = 1113257 mem = 536879361 peak = 902414336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 302474, 9, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 192, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (172.47, 95.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 751)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394)
[M::mem_pestat] mean and std.dev: (164.22, 69.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 488)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 761898 sequences (100000243 bp) in 3.599 CPU sec, 3.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 302070, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 257, 910)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2384)
[M::mem_pestat] mean and std.dev: (608.90, 659.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3246)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (164.99, 69.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 230, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250)
[M::mem_pestat] mean and std.dev: (336.60, 261.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1609)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 761318 sequences (100000009 bp) in 2.527 CPU sec, 2.566 real sec...
[M::mem_process_seqs] Processed 764494 reads in 241.437 CPU sec, 7.731 real sec
[M::mem_process_seqs] Processed 763096 reads in 171.604 CPU sec, 5.493 real sec
sorting block 14, #reads = 1110202 mem = 536879983 peak = 902414336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 301759, 13, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 252, 821)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2223)
[M::mem_pestat] mean and std.dev: (492.75, 530.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2924)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401)
[M::mem_pestat] mean and std.dev: (166.22, 70.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 497)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (636, 1120, 2967)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7629)
[M::mem_pestat] mean and std.dev: (1520.23, 1176.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9960)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 566, 1165)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3091)
[M::mem_pestat] mean and std.dev: (904.07, 859.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4342)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 760992 sequences (100000271 bp) in 3.577 CPU sec, 3.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 301695, 7, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 371, 1420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3988)
[M::mem_pestat] mean and std.dev: (567.82, 805.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5272)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 153, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401)
[M::mem_pestat] mean and std.dev: (166.42, 70.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 497)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 267, 981)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527)
[M::mem_pestat] mean and std.dev: (506.86, 466.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3300)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 761646 sequences (100000294 bp) in 2.412 CPU sec, 2.438 real sec...
[M::mem_process_seqs] Processed 761898 reads in 242.598 CPU sec, 7.781 real sec
sorting block 15, #reads = 1108945 mem = 536879718 peak = 902414336
[M::mem_process_seqs] Processed 761318 reads in 171.689 CPU sec, 5.531 real sec
sorting block 16, #reads = 1109039 mem = 536882087 peak = 927301632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 301589, 5, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 250, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1815)
[M::mem_pestat] mean and std.dev: (432.88, 322.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2354)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 152, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (166.21, 70.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (325, 522, 659)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327)
[M::mem_pestat] mean and std.dev: (442.64, 259.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1661)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 761134 sequences (100000138 bp) in 2.739 CPU sec, 3.061 real sec...
[M::process] read 761706 sequences (100000292 bp) in 2.673 CPU sec, 2.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 301522, 8, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 185, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (255.26, 180.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (165.89, 70.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 760992 reads in 243.171 CPU sec, 7.813 real sec
sorting block 17, #reads = 1108463 mem = 536879752 peak = 927301632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 302089, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 354, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1135)
[M::mem_pestat] mean and std.dev: (331.79, 183.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1451)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 152, 209)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399)
[M::mem_pestat] mean and std.dev: (166.21, 70.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 494)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 720, 2160)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6088)
[M::mem_pestat] mean and std.dev: (617.30, 598.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8052)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 767742 sequences (100000174 bp) in 3.035 CPU sec, 3.090 real sec...
[M::mem_process_seqs] Processed 761134 reads in 138.905 CPU sec, 4.433 real sec
[M::mem_process_seqs] Processed 761646 reads in 366.788 CPU sec, 11.755 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 302082, 11, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (165.72, 69.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1002, 1192, 2482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5442)
[M::mem_pestat] mean and std.dev: (1246.70, 937.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6922)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 18, #reads = 1107729 mem = 536882585 peak = 927301632
[M::process] read 768408 sequences (100000173 bp) in 3.046 CPU sec, 3.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 304028, 13, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 334, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1917)
[M::mem_pestat] mean and std.dev: (410.21, 349.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2507)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.35, 67.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (667, 1002, 2494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6148)
[M::mem_pestat] mean and std.dev: (1250.58, 891.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7975)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 768240 sequences (100000230 bp) in 2.370 CPU sec, 2.372 real sec...
[M::mem_process_seqs] Processed 761706 reads in 278.230 CPU sec, 8.951 real sec
[M::mem_process_seqs] Processed 767742 reads in 173.776 CPU sec, 5.581 real sec
sorting block 19, #reads = 1107373 mem = 536881202 peak = 927301632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 303946, 15, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 185, 247)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479)
[M::mem_pestat] mean and std.dev: (173.58, 69.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 595)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.71, 66.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (353, 359, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 869)
[M::mem_pestat] mean and std.dev: (391.36, 213.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 462, 1156)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3020)
[M::mem_pestat] mean and std.dev: (931.45, 962.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4780)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 767440 sequences (100000145 bp) in 3.582 CPU sec, 3.972 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 304538, 11, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 255, 572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1452)
[M::mem_pestat] mean and std.dev: (259.45, 148.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1892)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.22, 67.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (364, 389, 1942)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5098)
[M::mem_pestat] mean and std.dev: (1044.90, 1403.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6676)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 283, 1059)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2857)
[M::mem_pestat] mean and std.dev: (463.53, 410.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3756)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 766678 sequences (100000187 bp) in 2.503 CPU sec, 2.512 real sec...
[M::mem_process_seqs] Processed 768408 reads in 244.511 CPU sec, 7.863 real sec
sorting block 20, #reads = 1110492 mem = 536880347 peak = 927301632
[M::mem_process_seqs] Processed 768240 reads in 170.885 CPU sec, 5.513 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 304399, 11, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (113, 168, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (170.75, 81.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387)
[M::mem_pestat] mean and std.dev: (161.87, 67.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (256, 577, 5100)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14788)
[M::mem_pestat] mean and std.dev: (1670.45, 2195.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19632)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 278, 830)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2244)
[M::mem_pestat] mean and std.dev: (401.50, 360.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2951)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 21, #reads = 1112267 mem = 536883093 peak = 942739456
[M::process] read 765486 sequences (100000240 bp) in 3.540 CPU sec, 3.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 303692, 7, 27)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (161.99, 67.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 246, 1093)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2867)
[M::mem_pestat] mean and std.dev: (485.35, 472.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3754)
[M::mem_pestat] skip orientation RR
[M::process] read 764980 sequences (100000190 bp) in 2.465 CPU sec, 2.662 real sec...
[M::mem_process_seqs] Processed 767440 reads in 245.921 CPU sec, 7.981 real sec
[M::mem_process_seqs] Processed 766678 reads in 171.677 CPU sec, 5.565 real sec
sorting block 22, #reads = 1111467 mem = 536878489 peak = 942739456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 303394, 17, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 303, 1855)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5217)
[M::mem_pestat] mean and std.dev: (794.30, 1073.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6898)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (162.80, 68.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 515, 1303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3671)
[M::mem_pestat] mean and std.dev: (675.00, 765.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4855)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 188, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1890)
[M::mem_pestat] mean and std.dev: (456.72, 443.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2468)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 764626 sequences (100000077 bp) in 3.430 CPU sec, 3.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 303255, 9, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 408, 638)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424)
[M::mem_pestat] mean and std.dev: (451.88, 281.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1817)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.23, 68.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 430, 1254)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3452)
[M::mem_pestat] mean and std.dev: (595.15, 496.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4551)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 763674 sequences (100000070 bp) in 2.497 CPU sec, 2.509 real sec...
[M::mem_process_seqs] Processed 765486 reads in 244.138 CPU sec, 7.834 real sec
[M::mem_process_seqs] Processed 764980 reads in 172.261 CPU sec, 5.541 real sec
sorting block 23, #reads = 1112408 mem = 536878908 peak = 942739456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 303153, 6, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 284, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (86, 416)
[M::mem_pestat] mean and std.dev: (230.00, 70.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 513)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.66, 68.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 841, 1532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4162)
[M::mem_pestat] mean and std.dev: (937.89, 878.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5477)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 763482 sequences (100000168 bp) in 3.248 CPU sec, 3.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 302502, 8, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 147, 940)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2546)
[M::mem_pestat] mean and std.dev: (488.64, 526.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3349)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389)
[M::mem_pestat] mean and std.dev: (163.75, 68.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 481)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 476, 853)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2177)
[M::mem_pestat] mean and std.dev: (531.91, 507.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2839)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 762674 sequences (100000153 bp) in 2.260 CPU sec, 2.297 real sec...
[M::mem_process_seqs] Processed 764626 reads in 239.755 CPU sec, 7.711 real sec
sorting block 24, #reads = 1110282 mem = 536879090 peak = 942739456
[M::mem_process_seqs] Processed 763674 reads in 169.808 CPU sec, 5.437 real sec
sorting block 25, #reads = 1110283 mem = 536879108 peak = 960327680
[M::process] read 762954 sequences (100000158 bp) in 3.539 CPU sec, 3.869 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 301741, 9, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 256, 1577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4461)
[M::mem_pestat] mean and std.dev: (735.52, 983.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5903)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (164.47, 68.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 212, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (267.05, 204.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 762096 sequences (100000143 bp) in 2.585 CPU sec, 2.595 real sec...
[M::mem_process_seqs] Processed 762674 reads in 141.161 CPU sec, 4.481 real sec
sorting block 26, #reads = 1108448 mem = 536886502 peak = 960327680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 302123, 10, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 315, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1779)
[M::mem_pestat] mean and std.dev: (343.00, 239.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2317)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (164.00, 68.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 853, 2054)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5890)
[M::mem_pestat] mean and std.dev: (1010.50, 914.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7808)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 251, 868)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2306)
[M::mem_pestat] mean and std.dev: (549.90, 622.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3040)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 302914, 9, 24)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (164.91, 69.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 373, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (321.77, 190.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1368)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 763482 reads in 381.670 CPU sec, 12.257 real sec
[M::process] read 761508 sequences (100000193 bp) in 2.530 CPU sec, 2.558 real sec...
[M::mem_process_seqs] Processed 762954 reads in 208.402 CPU sec, 6.738 real sec
sorting block 27, #reads = 1109127 mem = 536880463 peak = 960327680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 302217, 4, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 226, 1050)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2828)
[M::mem_pestat] mean and std.dev: (547.67, 557.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3717)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (165.24, 69.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (421, 1203, 6148)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17602)
[M::mem_pestat] mean and std.dev: (3067.84, 3066.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 23329)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 762584 sequences (100000245 bp) in 3.284 CPU sec, 3.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 302180, 13, 23)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 208)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398)
[M::mem_pestat] mean and std.dev: (165.80, 69.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (23, 628, 4687)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14015)
[M::mem_pestat] mean and std.dev: (1951.31, 2196.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18679)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 208, 847)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2189)
[M::mem_pestat] mean and std.dev: (340.32, 308.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2860)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 769376 sequences (100000161 bp) in 2.534 CPU sec, 2.538 real sec...
[M::mem_process_seqs] Processed 762096 reads in 242.915 CPU sec, 7.793 real sec
[M::mem_process_seqs] Processed 761508 reads in 172.926 CPU sec, 5.546 real sec
sorting block 28, #reads = 1109166 mem = 536880062 peak = 960327680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 302283, 9, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 574, 1002)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588)
[M::mem_pestat] mean and std.dev: (659.90, 559.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3381)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (164.90, 69.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 486, 886)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2344)
[M::mem_pestat] mean and std.dev: (520.24, 383.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3073)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 768338 sequences (100000043 bp) in 3.301 CPU sec, 3.626 real sec...
[M::process] read 766900 sequences (100000062 bp) in 2.390 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304118, 18, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 161, 802)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150)
[M::mem_pestat] mean and std.dev: (191.58, 185.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2824)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.16, 66.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 829, 1094)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2948)
[M::mem_pestat] mean and std.dev: (625.88, 472.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3875)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 401, 3714)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10842)
[M::mem_pestat] mean and std.dev: (1933.42, 2446.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14406)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 762584 reads in 244.899 CPU sec, 7.849 real sec
sorting block 29, #reads = 1108199 mem = 536884482 peak = 960327680
[M::mem_process_seqs] Processed 769376 reads in 171.851 CPU sec, 5.518 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 304308, 12, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 249, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (251.50, 185.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1369)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384)
[M::mem_pestat] mean and std.dev: (161.16, 66.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 475)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (583, 2837, 3831)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10327)
[M::mem_pestat] mean and std.dev: (2505.08, 2309.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13575)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 252, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (263.46, 126.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 30, #reads = 1109949 mem = 536884561 peak = 960327680
[M::process] read 766844 sequences (100000007 bp) in 3.610 CPU sec, 3.822 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 304062, 17, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (161.95, 67.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (281, 771, 3909)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11165)
[M::mem_pestat] mean and std.dev: (2376.06, 3249.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15373)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (116, 134, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367)
[M::mem_pestat] mean and std.dev: (269.94, 286.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1784)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 766028 sequences (100000132 bp) in 2.786 CPU sec, 2.812 real sec...
[M::mem_process_seqs] Processed 768338 reads in 238.739 CPU sec, 7.643 real sec
[M::mem_process_seqs] Processed 766900 reads in 167.853 CPU sec, 5.393 real sec
sorting block 31, #reads = 1113093 mem = 536880815 peak = 960327680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 303509, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 220, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (231.73, 93.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387)
[M::mem_pestat] mean and std.dev: (162.13, 67.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 479)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 297, 1178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3262)
[M::mem_pestat] mean and std.dev: (377.80, 360.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4304)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 764766 sequences (100000095 bp) in 3.729 CPU sec, 3.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 303262, 12, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 1270, 3870)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11312)
[M::mem_pestat] mean and std.dev: (2045.60, 2296.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15033)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (162.56, 67.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (368, 831, 2880)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7904)
[M::mem_pestat] mean and std.dev: (1016.36, 983.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10416)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 360, 1346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3674)
[M::mem_pestat] mean and std.dev: (753.76, 801.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4838)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 764788 sequences (100000115 bp) in 2.394 CPU sec, 2.399 real sec...
[M::mem_process_seqs] Processed 766844 reads in 240.981 CPU sec, 7.707 real sec
sorting block 32, #reads = 1113033 mem = 536882909 peak = 960327680
[M::mem_process_seqs] Processed 766028 reads in 171.205 CPU sec, 5.480 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 302761, 10, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 277, 779)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071)
[M::mem_pestat] mean and std.dev: (350.65, 304.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2717)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.54, 68.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (690, 1403, 1595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3405)
[M::mem_pestat] mean and std.dev: (1135.67, 528.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4310)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 253, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (285.00, 153.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 764404 sequences (100000097 bp) in 3.582 CPU sec, 3.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 302715, 12, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (108, 147, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (178.15, 116.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.27, 68.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (72, 522, 1095)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3141)
[M::mem_pestat] mean and std.dev: (580.91, 717.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4164)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 371, 1853)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5087)
[M::mem_pestat] mean and std.dev: (531.36, 567.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6704)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 763834 sequences (100000048 bp) in 2.593 CPU sec, 2.598 real sec...
[M::mem_process_seqs] Processed 764766 reads in 241.503 CPU sec, 7.730 real sec
sorting block 33, #reads = 1111746 mem = 536880464 peak = 960327680
[M::mem_process_seqs] Processed 764788 reads in 170.632 CPU sec, 5.459 real sec
sorting block 34, #reads = 1110711 mem = 536882624 peak = 1003991040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 302159, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 160, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (54, 304)
[M::mem_pestat] mean and std.dev: (151.56, 29.75)
[M::mem_pestat] low and high boundaries for proper pairs: (4, 354)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391)
[M::mem_pestat] mean and std.dev: (163.42, 68.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 484)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 314, 1126)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3038)
[M::mem_pestat] mean and std.dev: (424.37, 419.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3994)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 764478 sequences (100000220 bp) in 3.069 CPU sec, 3.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 302758, 10, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 1116, 1748)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4876)
[M::mem_pestat] mean and std.dev: (767.18, 631.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6440)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386)
[M::mem_pestat] mean and std.dev: (163.04, 67.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 477)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (338, 766, 1821)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4787)
[M::mem_pestat] mean and std.dev: (1325.20, 1255.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6345)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 208, 1072)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2916)
[M::mem_pestat] mean and std.dev: (529.19, 620.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3838)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 763808 sequences (100000261 bp) in 2.318 CPU sec, 2.330 real sec...
[M::mem_process_seqs] Processed 764404 reads in 241.001 CPU sec, 7.746 real sec
[M::mem_process_seqs] Processed 763834 reads in 170.442 CPU sec, 5.463 real sec
sorting block 35, #reads = 1109389 mem = 536881461 peak = 1003991040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 302885, 9, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 148, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (169.30, 86.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (163.06, 67.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (125, 199, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163)
[M::mem_pestat] mean and std.dev: (285.50, 225.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1509)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 762864 sequences (100000039 bp) in 3.463 CPU sec, 3.574 real sec...
[M::process] read 763208 sequences (100000038 bp) in 2.476 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 302994, 9, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 294, 1136)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3116)
[M::mem_pestat] mean and std.dev: (506.22, 472.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4106)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389)
[M::mem_pestat] mean and std.dev: (163.38, 67.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 481)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 130, 160)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 252)
[M::mem_pestat] mean and std.dev: (133.42, 24.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 298)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 764478 reads in 237.799 CPU sec, 7.597 real sec
sorting block 36, #reads = 1109340 mem = 536886305 peak = 1003991040
[M::mem_process_seqs] Processed 763808 reads in 168.813 CPU sec, 5.413 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 302030, 16, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 273, 1891)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5399)
[M::mem_pestat] mean and std.dev: (854.00, 1142.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7153)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (164.37, 68.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (346, 892, 2345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6343)
[M::mem_pestat] mean and std.dev: (1066.60, 1081.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8342)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 243, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (263.45, 155.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 37, #reads = 1109525 mem = 536881374 peak = 1003991040
[M::process] read 762614 sequences (100000091 bp) in 3.436 CPU sec, 3.726 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 302038, 9, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 391, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1531)
[M::mem_pestat] mean and std.dev: (417.18, 381.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1986)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (164.29, 68.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 309, 1503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4123)
[M::mem_pestat] mean and std.dev: (1022.56, 1225.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5923)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 765570 sequences (100000041 bp) in 2.395 CPU sec, 2.412 real sec...
[M::mem_process_seqs] Processed 762864 reads in 243.458 CPU sec, 7.824 real sec
[M::mem_process_seqs] Processed 763208 reads in 173.443 CPU sec, 5.558 real sec
sorting block 38, #reads = 1109858 mem = 536880723 peak = 1003991040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 302280, 13, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 510, 1188)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3246)
[M::mem_pestat] mean and std.dev: (639.00, 682.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4275)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (164.90, 69.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 480, 1658)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4644)
[M::mem_pestat] mean and std.dev: (672.46, 615.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6137)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 319, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1545)
[M::mem_pestat] mean and std.dev: (406.24, 328.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2004)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 773496 sequences (100000090 bp) in 3.828 CPU sec, 4.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 302945, 12, 26)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (162.42, 67.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (78, 938, 1344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3876)
[M::mem_pestat] mean and std.dev: (757.08, 707.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5142)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 315, 701)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729)
[M::mem_pestat] mean and std.dev: (347.61, 251.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2243)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 771844 sequences (100000140 bp) in 2.611 CPU sec, 2.613 real sec...
[M::mem_process_seqs] Processed 762614 reads in 249.726 CPU sec, 8.027 real sec
[M::mem_process_seqs] Processed 765570 reads in 179.699 CPU sec, 5.794 real sec
sorting block 39, #reads = 1107897 mem = 536880717 peak = 1003991040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 305840, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 175, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (182.33, 75.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 145, 195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365)
[M::mem_pestat] mean and std.dev: (156.42, 62.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 833, 1045)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2859)
[M::mem_pestat] mean and std.dev: (600.58, 542.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3766)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 770740 sequences (100000144 bp) in 3.218 CPU sec, 3.570 real sec...
[M::process] read 770526 sequences (100000227 bp) in 2.303 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 305341, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 351, 844)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2156)
[M::mem_pestat] mean and std.dev: (565.20, 559.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2812)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 146, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368)
[M::mem_pestat] mean and std.dev: (157.18, 63.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 454)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 305, 971)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609)
[M::mem_pestat] mean and std.dev: (695.32, 705.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3519)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 773496 reads in 253.433 CPU sec, 8.157 real sec
sorting block 40, #reads = 1107747 mem = 536883229 peak = 1003991040
[M::mem_process_seqs] Processed 771844 reads in 175.892 CPU sec, 5.674 real sec
sorting block 41, #reads = 1112545 mem = 536878676 peak = 1007968256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 304898, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 270, 693)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1827)
[M::mem_pestat] mean and std.dev: (532.70, 565.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2794)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371)
[M::mem_pestat] mean and std.dev: (157.80, 63.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 458)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 251, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (264.08, 129.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 769374 sequences (100000075 bp) in 3.311 CPU sec, 3.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 304973, 11, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 341, 712)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828)
[M::mem_pestat] mean and std.dev: (515.21, 465.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2386)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 146, 197)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369)
[M::mem_pestat] mean and std.dev: (158.02, 63.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 455)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (265, 350, 1078)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2704)
[M::mem_pestat] mean and std.dev: (494.40, 358.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3517)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 217, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084)
[M::mem_pestat] mean and std.dev: (259.70, 171.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1397)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 768766 sequences (100000054 bp) in 2.605 CPU sec, 2.649 real sec...
[M::mem_process_seqs] Processed 770740 reads in 245.386 CPU sec, 7.895 real sec
[M::mem_process_seqs] Processed 770526 reads in 172.931 CPU sec, 5.555 real sec
sorting block 42, #reads = 1113173 mem = 536885078 peak = 1007968256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 305127, 9, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 626, 1260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3460)
[M::mem_pestat] mean and std.dev: (706.84, 645.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4560)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375)
[M::mem_pestat] mean and std.dev: (159.28, 64.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 198, 1411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4009)
[M::mem_pestat] mean and std.dev: (706.50, 751.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5308)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 769546 sequences (100000151 bp) in 3.246 CPU sec, 3.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 303920, 11, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 446, 1344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3754)
[M::mem_pestat] mean and std.dev: (593.07, 609.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4959)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (159.74, 65.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (287, 631, 3229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9113)
[M::mem_pestat] mean and std.dev: (2218.91, 2738.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13175)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 214, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1790)
[M::mem_pestat] mean and std.dev: (427.46, 367.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2319)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 767420 sequences (100000107 bp) in 2.361 CPU sec, 2.391 real sec...
[M::mem_process_seqs] Processed 769374 reads in 248.857 CPU sec, 8.050 real sec
sorting block 43, #reads = 1112762 mem = 536876108 peak = 1007968256
[M::mem_process_seqs] Processed 768766 reads in 178.526 CPU sec, 5.767 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 304917, 11, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 283, 1734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4898)
[M::mem_pestat] mean and std.dev: (845.92, 1059.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6480)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 147, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372)
[M::mem_pestat] mean and std.dev: (158.61, 64.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 459)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 257, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (213.40, 152.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 461, 2378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6840)
[M::mem_pestat] mean and std.dev: (961.08, 1153.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9071)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 767674 sequences (100000235 bp) in 3.179 CPU sec, 3.641 real sec...
sorting block 44, #reads = 1111210 mem = 536881906 peak = 1007968256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 303800, 10, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 157, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522)
[M::mem_pestat] mean and std.dev: (152.78, 54.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (160.61, 65.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 622, 1668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4682)
[M::mem_pestat] mean and std.dev: (648.44, 638.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6189)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 694, 2099)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5941)
[M::mem_pestat] mean and std.dev: (1163.31, 1323.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7862)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 765340 sequences (100000031 bp) in 2.752 CPU sec, 2.783 real sec...
[M::mem_process_seqs] Processed 769546 reads in 250.735 CPU sec, 8.111 real sec
[M::mem_process_seqs] Processed 767420 reads in 177.841 CPU sec, 5.741 real sec
sorting block 45, #reads = 1111963 mem = 536881734 peak = 1007968256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 304006, 16, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 145, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526)
[M::mem_pestat] mean and std.dev: (174.40, 88.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 661)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.56, 66.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 393, 2360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6574)
[M::mem_pestat] mean and std.dev: (1165.81, 1339.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8681)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (104, 105, 271)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (170.33, 130.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 772)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 763374 sequences (100000116 bp) in 3.428 CPU sec, 3.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 303805, 18, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 583, 1115)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909)
[M::mem_pestat] mean and std.dev: (670.93, 620.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3806)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (162.82, 67.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 490, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151)
[M::mem_pestat] mean and std.dev: (423.83, 292.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1594)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 761958 sequences (100000126 bp) in 2.439 CPU sec, 2.497 real sec...
[M::mem_process_seqs] Processed 767674 reads in 250.481 CPU sec, 8.085 real sec
[M::mem_process_seqs] Processed 765340 reads in 177.377 CPU sec, 5.711 real sec
sorting block 46, #reads = 1111863 mem = 536881290 peak = 1007968256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 302443, 12, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 775, 1205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3365)
[M::mem_pestat] mean and std.dev: (577.47, 511.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4445)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392)
[M::mem_pestat] mean and std.dev: (164.13, 68.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 485)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (76, 612, 1585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4603)
[M::mem_pestat] mean and std.dev: (708.42, 664.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6112)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 328, 747)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1735)
[M::mem_pestat] mean and std.dev: (507.73, 390.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2229)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 761974 sequences (100000008 bp) in 3.443 CPU sec, 3.877 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 301566, 9, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (100, 654, 1018)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2854)
[M::mem_pestat] mean and std.dev: (386.18, 322.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3772)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 152, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (164.93, 69.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 391, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (352.75, 197.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 767198 sequences (100000061 bp) in 2.723 CPU sec, 2.732 real sec...
[M::mem_process_seqs] Processed 763374 reads in 254.484 CPU sec, 8.182 real sec
sorting block 47, #reads = 1107384 mem = 536889141 peak = 1007968256
[M::mem_process_seqs] Processed 761958 reads in 181.324 CPU sec, 5.826 real sec
sorting block 48, #reads = 1105404 mem = 536885108 peak = 1020899328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 301665, 11, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 631, 1057)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2857)
[M::mem_pestat] mean and std.dev: (647.21, 643.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3757)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 151, 207)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395)
[M::mem_pestat] mean and std.dev: (164.81, 69.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 489)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 528, 918)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2394)
[M::mem_pestat] mean and std.dev: (601.36, 529.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3132)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (411, 733, 1076)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2406)
[M::mem_pestat] mean and std.dev: (659.25, 372.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3071)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 775410 sequences (100000234 bp) in 3.498 CPU sec, 3.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 302915, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 244, 662)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1718)
[M::mem_pestat] mean and std.dev: (279.71, 208.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2246)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.93, 66.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 333, 1417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3885)
[M::mem_pestat] mean and std.dev: (896.00, 1038.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5119)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 774572 sequences (100000102 bp) in 2.703 CPU sec, 2.856 real sec...
[M::mem_process_seqs] Processed 761974 reads in 260.309 CPU sec, 8.386 real sec
[M::mem_process_seqs] Processed 767198 reads in 188.795 CPU sec, 6.075 real sec
sorting block 49, #reads = 1103374 mem = 536881871 peak = 1020899328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 306423, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 264, 2232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6372)
[M::mem_pestat] mean and std.dev: (937.26, 1051.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8442)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 144, 193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361)
[M::mem_pestat] mean and std.dev: (155.04, 61.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 445)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 478, 1055)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2857)
[M::mem_pestat] mean and std.dev: (540.56, 541.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3758)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 772272 sequences (100000045 bp) in 3.619 CPU sec, 3.974 real sec...
[M::process] read 770724 sequences (100000038 bp) in 2.802 CPU sec, 2.856 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 305379, 11, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 177, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239)
[M::mem_pestat] mean and std.dev: (290.64, 303.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1610)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 144, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364)
[M::mem_pestat] mean and std.dev: (155.86, 62.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 194, 1248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3438)
[M::mem_pestat] mean and std.dev: (814.45, 971.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4699)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 804, 1040)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2800)
[M::mem_pestat] mean and std.dev: (673.11, 575.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3680)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 775410 reads in 276.573 CPU sec, 8.886 real sec
sorting block 50, #reads = 1105279 mem = 536892496 peak = 1020899328
[M::mem_process_seqs] Processed 774572 reads in 199.342 CPU sec, 6.411 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 305068, 8, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 150, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116)
[M::mem_pestat] mean and std.dev: (243.52, 229.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1451)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 146, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368)
[M::mem_pestat] mean and std.dev: (157.33, 63.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 454)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 141, 659)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1757)
[M::mem_pestat] mean and std.dev: (338.41, 448.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2306)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 51, #reads = 1105016 mem = 536882719 peak = 1020899328
[M::process] read 770016 sequences (100000275 bp) in 3.793 CPU sec, 4.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 304816, 6, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374)
[M::mem_pestat] mean and std.dev: (158.59, 64.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 462)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 324, 751)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1825)
[M::mem_pestat] mean and std.dev: (373.33, 321.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2362)
[M::mem_pestat] skip orientation RR
[M::process] read 769104 sequences (100000219 bp) in 2.559 CPU sec, 2.597 real sec...
[M::mem_process_seqs] Processed 772272 reads in 272.883 CPU sec, 8.812 real sec
[M::mem_process_seqs] Processed 770724 reads in 193.521 CPU sec, 6.216 real sec
sorting block 52, #reads = 1107227 mem = 536878661 peak = 1020899328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 304601, 8, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 187, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256)
[M::mem_pestat] mean and std.dev: (304.23, 269.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1617)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375)
[M::mem_pestat] mean and std.dev: (159.17, 64.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 387, 765)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1911)
[M::mem_pestat] mean and std.dev: (485.94, 354.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2484)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 768720 sequences (100000011 bp) in 3.414 CPU sec, 3.845 real sec...
[M::process] read 767638 sequences (100000214 bp) in 2.782 CPU sec, 2.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 304216, 8, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 165, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515)
[M::mem_pestat] mean and std.dev: (342.83, 337.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1974)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (159.84, 65.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 296, 1099)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2959)
[M::mem_pestat] mean and std.dev: (323.00, 273.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3889)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 770016 reads in 276.783 CPU sec, 8.918 real sec
sorting block 53, #reads = 1108462 mem = 536880205 peak = 1020899328
[M::mem_process_seqs] Processed 769104 reads in 201.273 CPU sec, 6.491 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304522, 6, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 204, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296)
[M::mem_pestat] mean and std.dev: (288.75, 251.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1687)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (160.08, 65.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 338, 718)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1700)
[M::mem_pestat] mean and std.dev: (456.50, 353.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2191)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 767254 sequences (100000217 bp) in 3.562 CPU sec, 4.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 303681, 11, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 325, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476)
[M::mem_pestat] mean and std.dev: (298.25, 180.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1915)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.65, 66.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 1101)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2997)
[M::mem_pestat] mean and std.dev: (496.55, 585.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3945)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 437, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1719)
[M::mem_pestat] mean and std.dev: (454.46, 380.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2241)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 765394 sequences (100000197 bp) in 2.533 CPU sec, 2.564 real sec...
[M::mem_process_seqs] Processed 768720 reads in 260.457 CPU sec, 8.442 real sec
sorting block 54, #reads = 1104744 mem = 536881021 peak = 1020899328
[M::mem_process_seqs] Processed 767638 reads in 187.858 CPU sec, 6.124 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 303710, 7, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 219, 625)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551)
[M::mem_pestat] mean and std.dev: (311.83, 219.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2014)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.90, 66.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 247, 1117)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2985)
[M::mem_pestat] mean and std.dev: (566.88, 589.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3919)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 55, #reads = 1108321 mem = 536880873 peak = 1025069056
[M::process] read 764292 sequences (100000142 bp) in 3.133 CPU sec, 3.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 303055, 9, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 304, 9406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 27902)
[M::mem_pestat] mean and std.dev: (2850.73, 4041.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 37150)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (162.15, 67.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 226, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (263.20, 140.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 763682 sequences (100000056 bp) in 2.896 CPU sec, 3.038 real sec...
[M::mem_process_seqs] Processed 767254 reads in 253.154 CPU sec, 8.254 real sec
[M::mem_process_seqs] Processed 765394 reads in 178.253 CPU sec, 5.787 real sec
sorting block 56, #reads = 1109478 mem = 536877670 peak = 1025069056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 302391, 10, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 186, 1457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4165)
[M::mem_pestat] mean and std.dev: (820.38, 1062.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5519)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (163.04, 67.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (345, 355, 915)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2055)
[M::mem_pestat] mean and std.dev: (436.00, 196.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2625)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 224, 1206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3288)
[M::mem_pestat] mean and std.dev: (592.50, 656.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4329)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 764232 sequences (100000173 bp) in 3.197 CPU sec, 3.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 302328, 18, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 201, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (199.31, 87.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386)
[M::mem_pestat] mean and std.dev: (162.99, 67.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 477)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 362, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1293)
[M::mem_pestat] mean and std.dev: (299.12, 196.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1672)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 230, 828)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2220)
[M::mem_pestat] mean and std.dev: (409.47, 373.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2916)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 772240 sequences (100000036 bp) in 2.610 CPU sec, 2.665 real sec...
[M::mem_process_seqs] Processed 764292 reads in 254.355 CPU sec, 8.239 real sec
sorting block 57, #reads = 1108478 mem = 536876992 peak = 1025069056
[M::mem_process_seqs] Processed 763682 reads in 181.484 CPU sec, 5.855 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 301684, 9, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 157, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583)
[M::mem_pestat] mean and std.dev: (198.42, 124.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (113, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386)
[M::mem_pestat] mean and std.dev: (162.57, 66.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 477)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 385, 691)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763)
[M::mem_pestat] mean and std.dev: (312.50, 185.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2299)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 58, #reads = 1105746 mem = 536878169 peak = 1025069056
[M::process] read 772660 sequences (100000002 bp) in 3.383 CPU sec, 3.633 real sec...
[M::process] read 770594 sequences (100000237 bp) in 2.583 CPU sec, 2.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 304751, 9, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 288, 932)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2470)
[M::mem_pestat] mean and std.dev: (506.00, 524.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3239)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 146, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368)
[M::mem_pestat] mean and std.dev: (157.12, 63.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 454)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 232, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1356)
[M::mem_pestat] mean and std.dev: (330.06, 251.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1762)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 764232 reads in 273.983 CPU sec, 8.835 real sec
[M::mem_process_seqs] Processed 772240 reads in 195.966 CPU sec, 6.295 real sec
sorting block 59, #reads = 1097155 mem = 536878997 peak = 1025069056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 305740, 8, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 276, 1024)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790)
[M::mem_pestat] mean and std.dev: (580.37, 593.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3673)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 146, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368)
[M::mem_pestat] mean and std.dev: (157.16, 63.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 454)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 239, 1133)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013)
[M::mem_pestat] mean and std.dev: (518.36, 587.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3953)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 769978 sequences (100000144 bp) in 3.205 CPU sec, 3.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 304691, 12, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 627, 1528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4282)
[M::mem_pestat] mean and std.dev: (901.60, 925.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5659)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377)
[M::mem_pestat] mean and std.dev: (158.99, 65.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 466)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (507, 575, 1159)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2463)
[M::mem_pestat] mean and std.dev: (666.33, 370.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3115)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 326, 1287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3577)
[M::mem_pestat] mean and std.dev: (612.28, 750.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4722)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 769108 sequences (100000068 bp) in 2.484 CPU sec, 2.525 real sec...
[M::mem_process_seqs] Processed 772660 reads in 252.662 CPU sec, 8.147 real sec
[M::mem_process_seqs] Processed 770594 reads in 175.989 CPU sec, 5.678 real sec
sorting block 60, #reads = 1110823 mem = 536879558 peak = 1025069056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 305174, 7, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 529, 1718)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4806)
[M::mem_pestat] mean and std.dev: (826.25, 968.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6350)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375)
[M::mem_pestat] mean and std.dev: (159.11, 64.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 767650 sequences (100000180 bp) in 3.688 CPU sec, 4.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 304894, 10, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375)
[M::mem_pestat] mean and std.dev: (159.50, 65.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (622, 1042, 1042)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882)
[M::mem_pestat] mean and std.dev: (732.50, 378.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2302)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 278, 1125)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2937)
[M::mem_pestat] mean and std.dev: (426.29, 384.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3843)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 768466 sequences (100000016 bp) in 2.725 CPU sec, 2.750 real sec...
[M::mem_process_seqs] Processed 769978 reads in 244.519 CPU sec, 7.904 real sec
sorting block 61, #reads = 1110836 mem = 536880183 peak = 1025069056
[M::mem_process_seqs] Processed 769108 reads in 174.260 CPU sec, 5.631 real sec
sorting block 62, #reads = 1111620 mem = 536886398 peak = 1025069056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 303941, 10, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 175, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226)
[M::mem_pestat] mean and std.dev: (286.09, 229.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1593)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.78, 66.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (98, 300, 869)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2411)
[M::mem_pestat] mean and std.dev: (610.20, 703.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3423)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 284, 2434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6930)
[M::mem_pestat] mean and std.dev: (1247.90, 1717.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9178)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 767320 sequences (100000067 bp) in 3.140 CPU sec, 3.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 304191, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (770, 1084, 1899)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4157)
[M::mem_pestat] mean and std.dev: (1200.36, 964.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5286)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.39, 66.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 287, 1328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3624)
[M::mem_pestat] mean and std.dev: (710.62, 778.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4772)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 766754 sequences (100000058 bp) in 2.486 CPU sec, 2.560 real sec...
[M::mem_process_seqs] Processed 767650 reads in 252.234 CPU sec, 8.158 real sec
[M::mem_process_seqs] Processed 768466 reads in 181.697 CPU sec, 5.874 real sec
sorting block 63, #reads = 1110926 mem = 536878377 peak = 1025069056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 303769, 15, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 369, 1382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3794)
[M::mem_pestat] mean and std.dev: (773.29, 872.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5000)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (160.90, 66.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (266, 554, 2054)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5630)
[M::mem_pestat] mean and std.dev: (934.36, 765.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7418)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 291, 2519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7171)
[M::mem_pestat] mean and std.dev: (1178.85, 1491.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9497)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 765708 sequences (100000253 bp) in 3.140 CPU sec, 3.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 303119, 12, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 187, 692)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1702)
[M::mem_pestat] mean and std.dev: (260.33, 177.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2207)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (161.40, 66.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (425, 964, 1384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302)
[M::mem_pestat] mean and std.dev: (783.70, 400.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4261)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 182, 822)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2200)
[M::mem_pestat] mean and std.dev: (447.31, 530.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2889)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 764690 sequences (100000046 bp) in 2.776 CPU sec, 2.787 real sec...
[M::mem_process_seqs] Processed 767320 reads in 250.212 CPU sec, 8.083 real sec
sorting block 64, #reads = 1108455 mem = 536879482 peak = 1025069056
[M::mem_process_seqs] Processed 766754 reads in 177.450 CPU sec, 5.699 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 303092, 8, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 169, 757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1973)
[M::mem_pestat] mean and std.dev: (411.80, 412.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2581)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (162.31, 67.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 406, 1475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3931)
[M::mem_pestat] mean and std.dev: (771.47, 676.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5159)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 765378 sequences (100000014 bp) in 3.176 CPU sec, 3.460 real sec...
sorting block 65, #reads = 1109238 mem = 536881708 peak = 1025069056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 302457, 10, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 517, 1809)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4939)
[M::mem_pestat] mean and std.dev: (904.50, 1045.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6504)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (163.05, 67.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (299, 512, 1095)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2687)
[M::mem_pestat] mean and std.dev: (772.60, 744.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3750)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 914, 5185)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15133)
[M::mem_pestat] mean and std.dev: (1986.46, 2029.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20107)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 765436 sequences (100000148 bp) in 2.530 CPU sec, 2.586 real sec...
[M::mem_process_seqs] Processed 765708 reads in 255.765 CPU sec, 8.251 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 301933, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 260, 580)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420)
[M::mem_pestat] mean and std.dev: (270.54, 221.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1840)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (162.22, 67.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 287, 1118)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3028)
[M::mem_pestat] mean and std.dev: (499.20, 428.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3983)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 764898 sequences (100000177 bp) in 3.069 CPU sec, 3.071 real sec...
[M::mem_process_seqs] Processed 765378 reads in 147.989 CPU sec, 4.721 real sec
sorting block 66, #reads = 1106567 mem = 536883956 peak = 1025069056
[M::mem_process_seqs] Processed 764690 reads in 387.544 CPU sec, 12.503 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 302507, 12, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 391, 624)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1638)
[M::mem_pestat] mean and std.dev: (396.29, 381.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2145)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (162.18, 66.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (384, 575, 1093)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2511)
[M::mem_pestat] mean and std.dev: (592.18, 437.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3220)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 368, 686)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1662)
[M::mem_pestat] mean and std.dev: (427.57, 342.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2150)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 67, #reads = 1106292 mem = 536881225 peak = 1074122752
[M::process] read 773348 sequences (100000075 bp) in 3.340 CPU sec, 3.839 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 302092, 11, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 628, 2396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6926)
[M::mem_pestat] mean and std.dev: (1107.92, 1242.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9191)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (162.26, 66.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 378, 1679)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4707)
[M::mem_pestat] mean and std.dev: (539.44, 594.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6221)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 351, 1047)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2743)
[M::mem_pestat] mean and std.dev: (517.24, 524.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3591)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 765436 reads in 302.798 CPU sec, 9.825 real sec
[M::process] read 777308 sequences (100000080 bp) in 2.794 CPU sec, 2.802 real sec...
[M::mem_process_seqs] Processed 764898 reads in 192.812 CPU sec, 6.247 real sec
sorting block 68, #reads = 1104130 mem = 536879531 peak = 1074122752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 304996, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 257, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312)
[M::mem_pestat] mean and std.dev: (315.00, 235.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1697)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 775150 sequences (100000210 bp) in 3.142 CPU sec, 3.590 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (110, 145, 195)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365)
[M::mem_pestat] mean and std.dev: (156.38, 62.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 450)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 622, 949)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2585)
[M::mem_pestat] mean and std.dev: (491.31, 375.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3403)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 772282 sequences (100000107 bp) in 2.778 CPU sec, 2.792 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306964, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 354, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1266)
[M::mem_pestat] mean and std.dev: (323.64, 165.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1623)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 143, 192)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358)
[M::mem_pestat] mean and std.dev: (154.15, 61.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 441)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 263, 809)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071)
[M::mem_pestat] mean and std.dev: (448.53, 432.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2702)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 773348 reads in 294.958 CPU sec, 9.518 real sec
sorting block 69, #reads = 1096877 mem = 536880439 peak = 1074122752
[M::mem_process_seqs] Processed 777308 reads in 210.038 CPU sec, 6.767 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 306230, 8, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 345, 1650)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4538)
[M::mem_pestat] mean and std.dev: (556.08, 512.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5982)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 145, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364)
[M::mem_pestat] mean and std.dev: (155.78, 62.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 449)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 257, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (275.80, 180.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 771148 sequences (100000084 bp) in 3.385 CPU sec, 3.775 real sec...
[M::process] read 769700 sequences (100000110 bp) in 2.692 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305678, 9, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 380, 984)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2462)
[M::mem_pestat] mean and std.dev: (563.40, 495.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3201)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 146, 196)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368)
[M::mem_pestat] mean and std.dev: (157.22, 63.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 454)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 547, 1107)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2831)
[M::mem_pestat] mean and std.dev: (634.67, 464.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3693)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 775150 reads in 273.114 CPU sec, 8.780 real sec
sorting block 70, #reads = 1106314 mem = 536893880 peak = 1074122752
[M::mem_process_seqs] Processed 772282 reads in 191.675 CPU sec, 6.168 real sec
sorting block 71, #reads = 1106039 mem = 536877395 peak = 1094832128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 305450, 10, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 361, 1032)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2760)
[M::mem_pestat] mean and std.dev: (629.67, 736.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3624)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374)
[M::mem_pestat] mean and std.dev: (158.76, 64.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 462)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 941, 3239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9395)
[M::mem_pestat] mean and std.dev: (1956.30, 2622.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12473)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 187, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (218.81, 103.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 769214 sequences (100000101 bp) in 3.171 CPU sec, 3.574 real sec...
[M::process] read 767324 sequences (100000087 bp) in 2.583 CPU sec, 2.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 304385, 7, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 455, 1354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3780)
[M::mem_pestat] mean and std.dev: (678.71, 602.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4993)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375)
[M::mem_pestat] mean and std.dev: (159.43, 65.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 463)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 208, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344)
[M::mem_pestat] mean and std.dev: (279.50, 186.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1748)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 771148 reads in 263.907 CPU sec, 8.570 real sec
[M::mem_process_seqs] Processed 769700 reads in 187.517 CPU sec, 6.103 real sec
sorting block 72, #reads = 1110218 mem = 536878577 peak = 1094832128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 304152, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 192, 1847)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5223)
[M::mem_pestat] mean and std.dev: (670.25, 725.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6911)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378)
[M::mem_pestat] mean and std.dev: (159.80, 65.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 467)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 244, 961)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2483)
[M::mem_pestat] mean and std.dev: (595.45, 618.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3244)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 766824 sequences (100000148 bp) in 2.995 CPU sec, 3.271 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 303656, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381)
[M::mem_pestat] mean and std.dev: (160.80, 66.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 471)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 394, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311)
[M::mem_pestat] mean and std.dev: (357.08, 251.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1703)
[M::mem_pestat] skip orientation RR
[M::process] read 766896 sequences (100000221 bp) in 2.730 CPU sec, 2.753 real sec...
[M::mem_process_seqs] Processed 769214 reads in 264.043 CPU sec, 8.531 real sec
sorting block 73, #reads = 1106200 mem = 536878741 peak = 1094832128
[M::mem_process_seqs] Processed 767324 reads in 187.412 CPU sec, 6.054 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 303055, 10, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 176, 831)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2289)
[M::mem_pestat] mean and std.dev: (360.00, 383.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3018)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (160.87, 65.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (335, 466, 3368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9434)
[M::mem_pestat] mean and std.dev: (1799.60, 2303.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12467)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 235, 719)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1809)
[M::mem_pestat] mean and std.dev: (388.94, 298.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2354)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 74, #reads = 1106253 mem = 536878923 peak = 1096900608
[M::process] read 766070 sequences (100000238 bp) in 2.916 CPU sec, 3.317 real sec...
[M::process] read 765186 sequences (100000052 bp) in 2.523 CPU sec, 2.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 303074, 14, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (160.88, 65.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (95, 309, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394)
[M::mem_pestat] mean and std.dev: (253.38, 199.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1827)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 560, 1971)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5605)
[M::mem_pestat] mean and std.dev: (780.46, 789.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7422)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 766824 reads in 259.202 CPU sec, 8.364 real sec
[M::mem_process_seqs] Processed 766896 reads in 183.263 CPU sec, 5.927 real sec
sorting block 75, #reads = 1105766 mem = 536881885 peak = 1096900608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 303051, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 351, 2998)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8610)
[M::mem_pestat] mean and std.dev: (532.70, 829.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11416)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 149, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382)
[M::mem_pestat] mean and std.dev: (161.47, 66.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 472)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 228, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (263.09, 154.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 764816 sequences (100000233 bp) in 3.272 CPU sec, 3.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 303081, 13, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 530, 1324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3710)
[M::mem_pestat] mean and std.dev: (684.41, 803.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4903)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (162.12, 66.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (440, 732, 1513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3659)
[M::mem_pestat] mean and std.dev: (749.92, 505.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4732)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 311, 1016)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2708)
[M::mem_pestat] mean and std.dev: (492.33, 401.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3554)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 764890 sequences (100000242 bp) in 2.744 CPU sec, 2.758 real sec...
[M::mem_process_seqs] Processed 766070 reads in 252.692 CPU sec, 8.122 real sec
[M::mem_process_seqs] Processed 765186 reads in 177.713 CPU sec, 5.716 real sec
sorting block 76, #reads = 1106177 mem = 536881579 peak = 1096900608
[M::process] read 131754 sequences (17201181 bp) in 0.796 CPU sec, 0.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 302848, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 503, 1314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3670)
[M::mem_pestat] mean and std.dev: (610.65, 730.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4848)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385)
[M::mem_pestat] mean and std.dev: (162.41, 67.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 476)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 253, 1337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3655)
[M::mem_pestat] mean and std.dev: (504.40, 498.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4814)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 52082, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379)
[M::mem_pestat] mean and std.dev: (161.33, 65.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 468)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 302470, 11, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 210, 1022)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2838)
[M::mem_pestat] mean and std.dev: (443.00, 461.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3746)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 150, 204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388)
[M::mem_pestat] mean and std.dev: (162.63, 67.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 480)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 280, 630)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436)
[M::mem_pestat] mean and std.dev: (389.73, 201.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1839)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 203, 1113)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3039)
[M::mem_pestat] mean and std.dev: (382.33, 389.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4002)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 764816 reads in 267.016 CPU sec, 8.707 real sec
sorting block 77, #reads = 1109340 mem = 536877454 peak = 1096900608
[M::mem_process_seqs] Processed 131754 reads in 166.338 CPU sec, 5.395 real sec
[M::mem_process_seqs] Processed 764890 reads in 201.094 CPU sec, 6.595 real sec
sorting block 78, #reads = 1105588 mem = 536884237 peak = 1114124288
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 87505550
overall: 71409836032 mem 17903.404 user 372.541 sys 606.185 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_IWL-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:1029_IWL-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_IWL-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1029_IWL-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1029_IWL-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 606.225 sec; CPU: 18275.947 sec
sorting block 79, #reads = 557615 mem = 270386533 peak = 1114124288
uniq header count 1
merging 80 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 4852807916
output reads: 88159937
bam_mem_sort: 80 calls 58.947 user 1.051 sys 61.566 real
bam_write: 80 calls 62.557 user 3.999 sys 131.479 real
execute: 1 calls 35.497 user 3.750 sys 651.452 real
merge_files: 1 calls 35.464 user 3.730 sys 44.730 real
parse_chunk: 1273 calls 79.236 user 20.561 sys 125.313 real
read_chunk: 3466 calls 50.973 user 19.898 sys 555.670 real
sort_block: 1 calls 34.409 user 18.887 sys 530.993 real
write_chunk: 2191 calls 34.799 user 3.389 sys 43.370 real
overall: 3423928320 mem 1520.488 user 71.704 sys 651.472 real
ls: cannot access '*.cram': No such file or directory