.command.log - Workdir - DAQ:SENTIEON_BWAMEM (FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1;L007;0001)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cd/c6b3ba49d0afef2bf25900770828f8/.command.log
Size: 200.1 KB
Task: DAQ:SENTIEON_BWAMEM (FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1;L007;0001)
Attempt
Content

  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cd/c6b3ba49d0afef2bf25900770828f8/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ac/d9a8b84f6813e73e9654d4095917eb/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cd/c6b3ba49d0afef2bf25900770828f8/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ac/d9a8b84f6813e73e9654d4095917eb/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1/FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1473.189 CPU sec, 50.648 real sec
[M::process] read 677556 sequences (100000071 bp) in 0.950 CPU sec, 0.959 real sec...
[M::process] read 676978 sequences (100000171 bp) in 1.120 CPU sec, 1.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281413, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 392, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (300.56, 124.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 358, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (369.55, 167.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676692 sequences (100000204 bp) in 1.170 CPU sec, 1.173 real sec...
[M::process] read 676406 sequences (100000263 bp) in 1.180 CPU sec, 1.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280904, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 364, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (374.58, 168.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677556 reads in 145.223 CPU sec, 4.578 real sec
[M::mem_process_seqs] Processed 676978 reads in 90.429 CPU sec, 2.885 real sec
sorting block 0, #reads = 1065709 mem = 536878232 peak = 615763968
[M::process] read 676144 sequences (100000275 bp) in 1.382 CPU sec, 1.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281213, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 366, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (375.58, 167.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675718 sequences (100000289 bp) in 1.480 CPU sec, 1.479 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280897, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 371, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (380.21, 168.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281134, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 368, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (376.61, 167.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676692 reads in 205.221 CPU sec, 6.544 real sec
[M::mem_process_seqs] Processed 676144 reads in 152.386 CPU sec, 4.889 real sec
[M::mem_process_seqs] Processed 676406 reads in 165.261 CPU sec, 5.316 real sec
sorting block 1, #reads = 1065731 mem = 536879607 peak = 746311680
[M::process] read 675348 sequences (100000078 bp) in 1.851 CPU sec, 1.989 real sec...
sorting block 2, #reads = 1065894 mem = 536879774 peak = 842104832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280448, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (325, 398, 733)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549)
[M::mem_pestat] mean and std.dev: (460.10, 224.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1957)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 372, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (380.32, 167.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675344 sequences (100000085 bp) in 1.738 CPU sec, 1.753 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280676, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 375, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (382.57, 167.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675718 reads in 147.625 CPU sec, 4.676 real sec
[M::mem_process_seqs] Processed 675348 reads in 93.111 CPU sec, 2.919 real sec
[M::process] read 674942 sequences (100000139 bp) in 1.783 CPU sec, 1.830 real sec...
sorting block 3, #reads = 1064712 mem = 536878490 peak = 842104832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 280766, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 1067, 1242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3268)
[M::mem_pestat] mean and std.dev: (776.17, 467.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4281)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (386.07, 169.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674710 sequences (100000047 bp) in 1.722 CPU sec, 1.816 real sec...
[M::process] read 674802 sequences (100000276 bp) in 1.330 CPU sec, 1.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280403, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 381, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (388.69, 169.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675344 reads in 147.994 CPU sec, 4.708 real sec
[M::mem_process_seqs] Processed 674942 reads in 93.056 CPU sec, 2.957 real sec
sorting block 4, #reads = 1064868 mem = 536880225 peak = 871153664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280053, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (390.56, 169.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674862 sequences (100000003 bp) in 2.064 CPU sec, 2.179 real sec...
[M::process] read 674804 sequences (100000126 bp) in 1.669 CPU sec, 1.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279755, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 381, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (388.29, 168.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674710 reads in 151.627 CPU sec, 4.842 real sec
sorting block 5, #reads = 1064193 mem = 536881400 peak = 871153664
[M::mem_process_seqs] Processed 674802 reads in 95.373 CPU sec, 3.045 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280131, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 380, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (387.57, 167.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (432, 629, 629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 1023)
[M::mem_pestat] mean and std.dev: (525.36, 231.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1451)
[M::mem_pestat] skip orientation RR
sorting block 6, #reads = 1064830 mem = 536882481 peak = 889810944
[M::process] read 674686 sequences (100000035 bp) in 2.286 CPU sec, 2.474 real sec...
[M::process] read 674914 sequences (100000045 bp) in 1.720 CPU sec, 1.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280193, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (389.26, 168.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674862 reads in 153.625 CPU sec, 4.885 real sec
[M::mem_process_seqs] Processed 674804 reads in 96.727 CPU sec, 3.054 real sec
sorting block 7, #reads = 1064425 mem = 536879229 peak = 889810944
[M::process] read 674522 sequences (100000162 bp) in 1.700 CPU sec, 1.781 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280812, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 384, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (391.14, 170.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674398 sequences (100000254 bp) in 1.420 CPU sec, 1.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280249, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 377, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (385.62, 168.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674686 reads in 153.409 CPU sec, 4.886 real sec
[M::mem_process_seqs] Processed 674914 reads in 95.175 CPU sec, 3.033 real sec
sorting block 8, #reads = 1064700 mem = 536877620 peak = 889810944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280164, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 381, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (388.89, 167.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674356 sequences (100000047 bp) in 2.198 CPU sec, 2.329 real sec...
[M::process] read 674598 sequences (100000167 bp) in 1.440 CPU sec, 1.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280547, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (391.90, 169.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674522 reads in 151.893 CPU sec, 4.825 real sec
[M::mem_process_seqs] Processed 674398 reads in 93.923 CPU sec, 2.978 real sec
sorting block 9, #reads = 1065466 mem = 536877307 peak = 900182016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279816, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 379, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (386.11, 165.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674458 sequences (100000223 bp) in 2.063 CPU sec, 2.158 real sec...
[M::process] read 674528 sequences (100000241 bp) in 1.760 CPU sec, 1.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279759, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 375, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (383.89, 166.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674356 reads in 156.013 CPU sec, 4.954 real sec
sorting block 10, #reads = 1064735 mem = 536879383 peak = 900182016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279775, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 375, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (383.29, 165.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674492 sequences (100000285 bp) in 2.111 CPU sec, 2.115 real sec...
[M::mem_process_seqs] Processed 674458 reads in 86.710 CPU sec, 2.735 real sec
sorting block 11, #reads = 1063570 mem = 536881808 peak = 917110784
[M::mem_process_seqs] Processed 674598 reads in 213.975 CPU sec, 6.778 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 279837, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 465, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1505)
[M::mem_pestat] mean and std.dev: (433.50, 315.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1955)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 375, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (383.30, 165.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674798 sequences (100000052 bp) in 2.026 CPU sec, 2.153 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279717, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674990 sequences (100000284 bp) in 1.440 CPU sec, 1.441 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (257, 379, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (386.73, 166.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674528 reads in 171.214 CPU sec, 5.428 real sec
sorting block 12, #reads = 1063958 mem = 536880299 peak = 917110784
[M::mem_process_seqs] Processed 674492 reads in 98.895 CPU sec, 3.127 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280158, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 372, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (381.21, 165.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1062193 mem = 536877548 peak = 917110784
[M::process] read 674610 sequences (100000247 bp) in 2.389 CPU sec, 2.530 real sec...
[M::process] read 674770 sequences (100000113 bp) in 1.454 CPU sec, 1.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280048, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 373, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (382.40, 167.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674798 reads in 156.771 CPU sec, 4.979 real sec
[M::mem_process_seqs] Processed 674990 reads in 98.917 CPU sec, 3.130 real sec
sorting block 14, #reads = 1063746 mem = 536880602 peak = 917110784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280158, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 375, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (384.07, 166.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674632 sequences (100000287 bp) in 2.273 CPU sec, 2.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280643, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 375, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (383.98, 167.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674666 sequences (100000153 bp) in 1.810 CPU sec, 1.811 real sec...
[M::mem_process_seqs] Processed 674610 reads in 154.454 CPU sec, 4.895 real sec
[M::mem_process_seqs] Processed 674770 reads in 97.100 CPU sec, 3.057 real sec
sorting block 15, #reads = 1063419 mem = 536878603 peak = 917110784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279857, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 375, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (384.73, 167.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 333, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (281.86, 103.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation RR
[M::process] read 674280 sequences (100000096 bp) in 2.409 CPU sec, 2.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280265, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (389.86, 170.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674316 sequences (100000125 bp) in 1.799 CPU sec, 1.806 real sec...
[M::mem_process_seqs] Processed 674632 reads in 155.084 CPU sec, 4.911 real sec
[M::mem_process_seqs] Processed 674666 reads in 98.348 CPU sec, 3.108 real sec
sorting block 16, #reads = 1062845 mem = 536881620 peak = 937390080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280206, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (393.59, 172.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676128 sequences (100000060 bp) in 1.961 CPU sec, 2.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280508, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 263, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (241.70, 97.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (394.18, 173.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676744 sequences (100000128 bp) in 1.800 CPU sec, 1.793 real sec...
[M::mem_process_seqs] Processed 674280 reads in 151.681 CPU sec, 4.814 real sec
sorting block 17, #reads = 1062088 mem = 536877595 peak = 937390080
[M::mem_process_seqs] Processed 674316 reads in 95.747 CPU sec, 3.024 real sec
sorting block 18, #reads = 1064208 mem = 536877618 peak = 952582144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281041, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 370, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (380.84, 170.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676350 sequences (100000218 bp) in 2.438 CPU sec, 2.573 real sec...
[M::process] read 675922 sequences (100000177 bp) in 1.466 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280455, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 364, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (374.35, 168.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676128 reads in 154.179 CPU sec, 4.896 real sec
[M::mem_process_seqs] Processed 676744 reads in 98.033 CPU sec, 3.105 real sec
sorting block 19, #reads = 1064304 mem = 536882537 peak = 952582144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280324, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 366, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (376.48, 167.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675944 sequences (100000263 bp) in 2.071 CPU sec, 2.195 real sec...
[M::process] read 675778 sequences (100000294 bp) in 1.530 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280750, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 368, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (377.61, 166.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676350 reads in 151.484 CPU sec, 4.818 real sec
[M::mem_process_seqs] Processed 675922 reads in 94.542 CPU sec, 2.989 real sec
sorting block 20, #reads = 1066939 mem = 536879173 peak = 952582144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280617, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 366, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (376.05, 165.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675406 sequences (100000001 bp) in 2.393 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280478, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 369, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (378.05, 165.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675508 sequences (100000142 bp) in 1.730 CPU sec, 1.730 real sec...
[M::mem_process_seqs] Processed 675944 reads in 147.537 CPU sec, 4.677 real sec
[M::mem_process_seqs] Processed 675778 reads in 92.917 CPU sec, 2.930 real sec
sorting block 21, #reads = 1066791 mem = 536878778 peak = 952582144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280939, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 373, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (380.82, 166.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 286, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560)
[M::mem_pestat] mean and std.dev: (246.56, 109.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 697)
[M::mem_pestat] skip orientation RR
[M::process] read 675226 sequences (100000274 bp) in 2.011 CPU sec, 2.127 real sec...
[M::process] read 675322 sequences (100000137 bp) in 1.440 CPU sec, 1.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280717, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 374, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (381.93, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675406 reads in 151.671 CPU sec, 4.830 real sec
sorting block 22, #reads = 1066569 mem = 536876751 peak = 952582144
[M::mem_process_seqs] Processed 675508 reads in 95.870 CPU sec, 3.035 real sec
sorting block 23, #reads = 1065465 mem = 536877959 peak = 1044385792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280474, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 375, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (383.03, 167.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675158 sequences (100000176 bp) in 2.419 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280528, 0, 21)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (384.76, 167.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (294, 294, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (136, 531)
[M::mem_pestat] mean and std.dev: (291.67, 67.32)
[M::mem_pestat] low and high boundaries for proper pairs: (22, 610)
[M::mem_pestat] skip orientation RR
[M::process] read 674556 sequences (100000035 bp) in 1.750 CPU sec, 1.748 real sec...
[M::mem_process_seqs] Processed 675226 reads in 150.605 CPU sec, 4.774 real sec
[M::mem_process_seqs] Processed 675322 reads in 93.562 CPU sec, 2.949 real sec
sorting block 24, #reads = 1066884 mem = 536881840 peak = 1044385792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280178, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 377, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (384.76, 167.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674598 sequences (100000105 bp) in 2.303 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279207, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 380, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (388.18, 167.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674790 sequences (100000207 bp) in 1.730 CPU sec, 1.726 real sec...
[M::mem_process_seqs] Processed 675158 reads in 149.702 CPU sec, 4.747 real sec
[M::mem_process_seqs] Processed 674556 reads in 94.483 CPU sec, 2.980 real sec
sorting block 25, #reads = 1066498 mem = 536876292 peak = 1044385792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279735, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 381, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (388.52, 167.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674486 sequences (100000038 bp) in 2.651 CPU sec, 2.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280552, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 378, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (386.19, 166.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674598 reads in 152.065 CPU sec, 4.816 real sec
[M::process] read 674332 sequences (100000292 bp) in 1.760 CPU sec, 1.751 real sec...
sorting block 26, #reads = 1064639 mem = 536878916 peak = 1044385792
[M::mem_process_seqs] Processed 674790 reads in 96.341 CPU sec, 3.031 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279885, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 383, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (389.87, 168.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1065226 mem = 536878197 peak = 1044385792
[M::process] read 674634 sequences (100000060 bp) in 2.328 CPU sec, 2.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280253, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 381, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (389.24, 168.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674562 sequences (100000004 bp) in 1.780 CPU sec, 1.776 real sec...
[M::mem_process_seqs] Processed 674486 reads in 152.226 CPU sec, 4.827 real sec
[M::mem_process_seqs] Processed 674332 reads in 95.811 CPU sec, 3.013 real sec
sorting block 28, #reads = 1064707 mem = 536880647 peak = 1044385792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279964, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 374, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (382.64, 165.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674136 sequences (100000161 bp) in 2.644 CPU sec, 2.743 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280586, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 380, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (387.95, 167.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674634 reads in 149.477 CPU sec, 4.732 real sec
[M::process] read 674254 sequences (100000286 bp) in 1.806 CPU sec, 1.816 real sec...
[M::mem_process_seqs] Processed 674562 reads in 94.064 CPU sec, 2.958 real sec
sorting block 29, #reads = 1064210 mem = 536879398 peak = 1044385792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279992, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 380, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (387.82, 167.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674424 sequences (100000050 bp) in 2.366 CPU sec, 2.479 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280368, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 379, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (386.89, 167.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674136 reads in 150.709 CPU sec, 4.782 real sec
[M::process] read 675368 sequences (100000281 bp) in 1.854 CPU sec, 1.854 real sec...
[M::mem_process_seqs] Processed 674254 reads in 94.895 CPU sec, 2.981 real sec
sorting block 30, #reads = 1063859 mem = 536878439 peak = 1044385792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280530, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 382, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (389.53, 168.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674372 sequences (100000193 bp) in 2.287 CPU sec, 2.399 real sec...
[M::process] read 674964 sequences (100000188 bp) in 1.400 CPU sec, 1.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281308, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 371, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (381.45, 168.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 1042, 1042)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2762)
[M::mem_pestat] mean and std.dev: (694.00, 385.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3622)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674424 reads in 153.017 CPU sec, 4.866 real sec
sorting block 31, #reads = 1064238 mem = 536876643 peak = 1044385792
[M::mem_process_seqs] Processed 675368 reads in 94.932 CPU sec, 3.009 real sec
sorting block 32, #reads = 1064752 mem = 536878673 peak = 1044385792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280406, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 381, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (389.15, 168.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674670 sequences (100000032 bp) in 2.220 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279878, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 374, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (384.03, 167.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674774 sequences (100000153 bp) in 1.754 CPU sec, 1.755 real sec...
[M::mem_process_seqs] Processed 674372 reads in 152.933 CPU sec, 4.907 real sec
[M::mem_process_seqs] Processed 674964 reads in 96.153 CPU sec, 3.088 real sec
sorting block 33, #reads = 1064818 mem = 536879113 peak = 1044385792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280131, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (385.14, 167.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674900 sequences (100000058 bp) in 2.534 CPU sec, 2.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280545, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 377, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (386.21, 169.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 191, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (211.09, 73.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RR
[M::process] read 674642 sequences (100000255 bp) in 1.790 CPU sec, 1.789 real sec...
[M::mem_process_seqs] Processed 674670 reads in 154.092 CPU sec, 4.878 real sec
[M::mem_process_seqs] Processed 674774 reads in 96.005 CPU sec, 3.022 real sec
sorting block 34, #reads = 1063730 mem = 536877942 peak = 1044385792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281096, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 374, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (384.56, 168.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674590 sequences (100000152 bp) in 2.321 CPU sec, 2.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280446, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (386.25, 169.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674900 reads in 153.903 CPU sec, 4.892 real sec
[M::process] read 674596 sequences (100000136 bp) in 1.871 CPU sec, 1.884 real sec...
[M::mem_process_seqs] Processed 674642 reads in 98.845 CPU sec, 3.135 real sec
sorting block 35, #reads = 1064258 mem = 536878672 peak = 1044385792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280524, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (390.26, 171.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674454 sequences (100000195 bp) in 2.663 CPU sec, 2.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280689, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (393.99, 173.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674590 reads in 146.589 CPU sec, 4.662 real sec
sorting block 36, #reads = 1064647 mem = 536878445 peak = 1044385792
[M::mem_process_seqs] Processed 674596 reads in 92.982 CPU sec, 2.918 real sec
[M::process] read 675848 sequences (100000113 bp) in 1.922 CPU sec, 1.953 real sec...
sorting block 37, #reads = 1063015 mem = 536878989 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280754, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 387, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (395.58, 174.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677018 sequences (100000259 bp) in 2.035 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281214, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 371, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (381.74, 170.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674454 reads in 147.909 CPU sec, 4.700 real sec
[M::process] read 676562 sequences (100000294 bp) in 1.759 CPU sec, 1.766 real sec...
[M::mem_process_seqs] Processed 675848 reads in 94.642 CPU sec, 2.982 real sec
sorting block 38, #reads = 1062670 mem = 536885731 peak = 1106710528
[M::process] read 675900 sequences (100000266 bp) in 2.242 CPU sec, 2.339 real sec...
[M::process] read 675814 sequences (100000201 bp) in 1.430 CPU sec, 1.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281549, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 364, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (374.00, 167.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676562 reads in 80.046 CPU sec, 2.526 real sec
sorting block 39, #reads = 1066189 mem = 536879477 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281484, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 360, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (371.18, 166.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (309, 657, 684)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434)
[M::mem_pestat] mean and std.dev: (481.90, 228.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1809)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281573, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 367, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (376.27, 166.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675634 sequences (100000269 bp) in 1.781 CPU sec, 1.791 real sec...
[M::mem_process_seqs] Processed 677018 reads in 226.252 CPU sec, 7.178 real sec
[M::mem_process_seqs] Processed 675900 reads in 105.108 CPU sec, 3.346 real sec
sorting block 40, #reads = 1066916 mem = 536882612 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 281156, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 180, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 335)
[M::mem_pestat] mean and std.dev: (172.15, 47.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 401)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 367, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (377.08, 166.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675322 sequences (100000176 bp) in 2.175 CPU sec, 2.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280750, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 369, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (378.89, 166.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675814 reads in 143.444 CPU sec, 4.556 real sec
[M::process] read 675056 sequences (100000080 bp) in 1.840 CPU sec, 1.854 real sec...
[M::mem_process_seqs] Processed 675634 reads in 90.979 CPU sec, 2.861 real sec
sorting block 41, #reads = 1066408 mem = 536879639 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281127, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 374, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (381.84, 167.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675272 sequences (100000232 bp) in 2.500 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281368, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 375, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (383.49, 167.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675322 reads in 143.934 CPU sec, 4.565 real sec
[M::mem_process_seqs] Processed 675056 reads in 91.030 CPU sec, 2.857 real sec
[M::process] read 675096 sequences (100000109 bp) in 1.889 CPU sec, 1.912 real sec...
sorting block 42, #reads = 1065994 mem = 536876624 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281525, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 726, 772)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1866)
[M::mem_pestat] mean and std.dev: (518.80, 267.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2413)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (386.05, 169.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674658 sequences (100000122 bp) in 2.012 CPU sec, 2.122 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280382, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 381, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (389.04, 170.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674500 sequences (100000232 bp) in 1.440 CPU sec, 1.435 real sec...
[M::mem_process_seqs] Processed 675272 reads in 141.676 CPU sec, 4.510 real sec
sorting block 43, #reads = 1064720 mem = 536879064 peak = 1106710528
[M::mem_process_seqs] Processed 675096 reads in 90.190 CPU sec, 2.842 real sec
sorting block 44, #reads = 1065554 mem = 536879263 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 280422, 1, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 363, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 601)
[M::mem_pestat] mean and std.dev: (295.62, 92.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 720)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (391.70, 171.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674372 sequences (100000084 bp) in 2.241 CPU sec, 2.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280732, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 383, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (391.07, 170.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674658 reads in 143.162 CPU sec, 4.537 real sec
[M::process] read 674204 sequences (100000122 bp) in 1.854 CPU sec, 1.853 real sec...
[M::mem_process_seqs] Processed 674500 reads in 90.297 CPU sec, 2.842 real sec
sorting block 45, #reads = 1065304 mem = 536878980 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280790, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 384, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (392.27, 170.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674234 sequences (100000097 bp) in 2.236 CPU sec, 2.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280301, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 381, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (390.39, 170.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674372 reads in 143.541 CPU sec, 4.556 real sec
[M::process] read 674292 sequences (100000166 bp) in 1.913 CPU sec, 1.940 real sec...
[M::mem_process_seqs] Processed 674204 reads in 91.072 CPU sec, 2.854 real sec
sorting block 46, #reads = 1065132 mem = 536881090 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280126, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 383, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (392.29, 170.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674210 sequences (100000242 bp) in 2.400 CPU sec, 2.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280088, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 383, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (392.09, 170.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674294 sequences (100000168 bp) in 1.670 CPU sec, 1.674 real sec...
[M::mem_process_seqs] Processed 674234 reads in 148.661 CPU sec, 4.715 real sec
sorting block 47, #reads = 1064047 mem = 536887648 peak = 1106710528
[M::mem_process_seqs] Processed 674292 reads in 93.319 CPU sec, 2.939 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280000, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 382, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (390.85, 169.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1065901 mem = 536880965 peak = 1106710528
[M::process] read 674402 sequences (100000112 bp) in 2.267 CPU sec, 2.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280062, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 376, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (385.85, 167.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674210 reads in 144.840 CPU sec, 4.595 real sec
[M::process] read 674376 sequences (100000058 bp) in 1.813 CPU sec, 1.825 real sec...
[M::mem_process_seqs] Processed 674294 reads in 90.889 CPU sec, 2.852 real sec
sorting block 49, #reads = 1064826 mem = 536878335 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280229, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (389.19, 170.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674632 sequences (100000247 bp) in 2.119 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280827, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 375, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (386.53, 169.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674714 sequences (100000273 bp) in 1.480 CPU sec, 1.479 real sec...
[M::mem_process_seqs] Processed 674402 reads in 145.655 CPU sec, 4.640 real sec
[M::mem_process_seqs] Processed 674376 reads in 92.000 CPU sec, 2.908 real sec
sorting block 50, #reads = 1065170 mem = 536881131 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280030, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (387.45, 170.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674624 sequences (100000277 bp) in 1.992 CPU sec, 2.118 real sec...
[M::process] read 674776 sequences (100000154 bp) in 1.420 CPU sec, 1.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280515, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (385.20, 170.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 248, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (273.14, 135.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674632 reads in 148.113 CPU sec, 4.712 real sec
[M::mem_process_seqs] Processed 674714 reads in 92.771 CPU sec, 2.940 real sec
sorting block 51, #reads = 1065846 mem = 536882866 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279805, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 374, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (384.66, 169.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674810 sequences (100000155 bp) in 2.248 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280819, 0, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 373, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (383.31, 168.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (301, 301, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (376.07, 155.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RR
[M::process] read 674726 sequences (100000016 bp) in 1.740 CPU sec, 1.731 real sec...
[M::mem_process_seqs] Processed 674624 reads in 150.413 CPU sec, 4.771 real sec
sorting block 52, #reads = 1063707 mem = 536879534 peak = 1106710528
[M::mem_process_seqs] Processed 674776 reads in 95.214 CPU sec, 3.002 real sec
sorting block 53, #reads = 1063999 mem = 536880171 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280565, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (384.69, 169.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674788 sequences (100000043 bp) in 2.179 CPU sec, 2.278 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280557, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 371, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (382.01, 168.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674580 sequences (100000238 bp) in 1.550 CPU sec, 1.552 real sec...
[M::mem_process_seqs] Processed 674810 reads in 150.970 CPU sec, 4.797 real sec
[M::mem_process_seqs] Processed 674726 reads in 94.902 CPU sec, 2.996 real sec
sorting block 54, #reads = 1064245 mem = 536879998 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280113, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 375, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (385.43, 170.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674414 sequences (100000284 bp) in 2.334 CPU sec, 2.444 real sec...
[M::process] read 674458 sequences (100000116 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280700, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 378, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (388.63, 171.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674788 reads in 148.618 CPU sec, 4.720 real sec
[M::mem_process_seqs] Processed 674580 reads in 92.280 CPU sec, 2.922 real sec
sorting block 55, #reads = 1063731 mem = 536879008 peak = 1106710528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280704, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (393.62, 174.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674232 sequences (100000119 bp) in 2.155 CPU sec, 2.255 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280561, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (392.81, 173.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674394 sequences (100000218 bp) in 1.450 CPU sec, 1.446 real sec...
[M::mem_process_seqs] Processed 674414 reads in 145.447 CPU sec, 4.625 real sec
sorting block 56, #reads = 1064899 mem = 536878600 peak = 1106710528
[M::mem_process_seqs] Processed 674458 reads in 93.607 CPU sec, 2.956 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280805, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 391, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (400.78, 178.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676928 sequences (100000048 bp) in 2.268 CPU sec, 2.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280840, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 389, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (399.07, 177.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676356 sequences (100000286 bp) in 1.780 CPU sec, 1.777 real sec...
[M::mem_process_seqs] Processed 674232 reads in 144.904 CPU sec, 4.586 real sec
sorting block 57, #reads = 1063364 mem = 536879628 peak = 1106710528
[M::mem_process_seqs] Processed 674394 reads in 92.642 CPU sec, 2.927 real sec
sorting block 58, #reads = 1064373 mem = 536879126 peak = 1152225280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281094, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 362, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (373.56, 168.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675920 sequences (100000289 bp) in 2.330 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280134, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 366, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (377.22, 169.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675480 sequences (100000266 bp) in 1.740 CPU sec, 1.740 real sec...
[M::mem_process_seqs] Processed 676928 reads in 152.544 CPU sec, 4.838 real sec
[M::mem_process_seqs] Processed 676356 reads in 95.003 CPU sec, 2.992 real sec
sorting block 59, #reads = 1066528 mem = 536880786 peak = 1152225280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280530, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 374, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (384.76, 172.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 385, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (326.90, 107.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RR
[M::process] read 675218 sequences (100000094 bp) in 2.412 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280061, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (385.54, 171.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675030 sequences (100000273 bp) in 1.740 CPU sec, 1.738 real sec...
[M::mem_process_seqs] Processed 675920 reads in 148.571 CPU sec, 4.707 real sec
[M::mem_process_seqs] Processed 675480 reads in 93.023 CPU sec, 2.932 real sec
sorting block 60, #reads = 1066682 mem = 536880421 peak = 1152225280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279994, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 375, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (384.68, 169.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674992 sequences (100000213 bp) in 2.049 CPU sec, 2.148 real sec...
[M::process] read 674746 sequences (100000273 bp) in 1.410 CPU sec, 1.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280261, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 373, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (383.44, 168.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675218 reads in 147.042 CPU sec, 4.680 real sec
sorting block 61, #reads = 1066557 mem = 536878899 peak = 1152225280
[M::mem_process_seqs] Processed 675030 reads in 91.869 CPU sec, 2.907 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280284, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (388.85, 171.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 62, #reads = 1066106 mem = 536879287 peak = 1152225280
[M::process] read 674828 sequences (100000011 bp) in 2.406 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280531, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (392.27, 171.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674474 sequences (100000152 bp) in 1.711 CPU sec, 1.718 real sec...
[M::mem_process_seqs] Processed 674992 reads in 147.601 CPU sec, 4.675 real sec
[M::mem_process_seqs] Processed 674746 reads in 92.310 CPU sec, 2.912 real sec
sorting block 63, #reads = 1066780 mem = 536881437 peak = 1152225280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280681, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 385, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (393.18, 172.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674526 sequences (100000068 bp) in 2.268 CPU sec, 2.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279229, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (393.53, 172.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674828 reads in 146.083 CPU sec, 4.633 real sec
[M::process] read 674382 sequences (100000292 bp) in 1.740 CPU sec, 1.741 real sec...
[M::mem_process_seqs] Processed 674474 reads in 90.805 CPU sec, 2.855 real sec
sorting block 64, #reads = 1066393 mem = 536879821 peak = 1152225280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 279742, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 231, 624)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1410)
[M::mem_pestat] mean and std.dev: (376.09, 218.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1803)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (396.28, 173.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674366 sequences (100000171 bp) in 2.551 CPU sec, 2.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279465, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 387, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (395.02, 172.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674526 reads in 146.721 CPU sec, 4.645 real sec
[M::process] read 674498 sequences (100000297 bp) in 1.759 CPU sec, 1.761 real sec...
[M::mem_process_seqs] Processed 674382 reads in 90.950 CPU sec, 2.863 real sec
sorting block 65, #reads = 1065358 mem = 536879309 peak = 1152225280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280045, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 386, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (394.32, 171.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674384 sequences (100000023 bp) in 2.295 CPU sec, 2.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279617, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (392.79, 171.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674068 sequences (100000054 bp) in 1.730 CPU sec, 1.728 real sec...
[M::mem_process_seqs] Processed 674366 reads in 150.133 CPU sec, 4.761 real sec
sorting block 66, #reads = 1065418 mem = 536877801 peak = 1152225280
[M::mem_process_seqs] Processed 674498 reads in 93.932 CPU sec, 2.957 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280215, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (391.68, 170.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1064734 mem = 536877680 peak = 1152225280
[M::process] read 673762 sequences (100000005 bp) in 2.598 CPU sec, 2.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279886, 2, 20)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 383, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (391.40, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (590, 676, 676)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (418, 848)
[M::mem_pestat] mean and std.dev: (653.07, 38.03)
[M::mem_pestat] low and high boundaries for proper pairs: (332, 934)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674384 reads in 149.452 CPU sec, 4.732 real sec
[M::process] read 674500 sequences (100000076 bp) in 1.750 CPU sec, 1.753 real sec...
[M::mem_process_seqs] Processed 674068 reads in 93.691 CPU sec, 2.943 real sec
sorting block 68, #reads = 1064906 mem = 536880061 peak = 1152225280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279494, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 386, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (393.60, 169.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675094 sequences (100000255 bp) in 2.194 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280149, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (387.00, 169.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675218 sequences (100000290 bp) in 1.770 CPU sec, 1.774 real sec...
[M::mem_process_seqs] Processed 673762 reads in 149.440 CPU sec, 4.741 real sec
[M::mem_process_seqs] Processed 674500 reads in 94.356 CPU sec, 2.972 real sec
sorting block 69, #reads = 1064178 mem = 536878992 peak = 1152225280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279990, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 372, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (380.96, 167.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674884 sequences (100000011 bp) in 2.370 CPU sec, 2.481 real sec...
[M::process] read 674884 sequences (100000007 bp) in 1.440 CPU sec, 1.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280790, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 369, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (379.42, 166.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675094 reads in 149.429 CPU sec, 4.747 real sec
[M::mem_process_seqs] Processed 675218 reads in 92.884 CPU sec, 2.942 real sec
sorting block 70, #reads = 1064612 mem = 536878970 peak = 1152225280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279463, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (384.09, 168.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675124 sequences (100000254 bp) in 2.029 CPU sec, 2.123 real sec...
[M::process] read 675164 sequences (100000111 bp) in 1.390 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280374, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 373, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (382.76, 167.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674884 reads in 150.700 CPU sec, 4.800 real sec
sorting block 71, #reads = 1065755 mem = 536880116 peak = 1152225280
[M::process] read 674962 sequences (100000012 bp) in 2.262 CPU sec, 2.271 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280671, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 368, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (379.40, 166.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675164 reads in 77.896 CPU sec, 2.521 real sec
sorting block 72, #reads = 1064164 mem = 536883082 peak = 1182740480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280396, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 371, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (381.33, 167.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675124 reads in 160.661 CPU sec, 5.540 real sec
[M::mem_process_seqs] Processed 674884 reads in 306.288 CPU sec, 10.204 real sec
sorting block 73, #reads = 1065535 mem = 536883512 peak = 1182740480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279939, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 371, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (381.23, 167.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 228, 228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (228, 228)
[M::mem_pestat] mean and std.dev: (228.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (228, 228)
[M::mem_pestat] skip orientation RR
[M::process] read 674578 sequences (100000262 bp) in 2.757 CPU sec, 2.932 real sec...
[M::mem_process_seqs] Processed 674962 reads in 96.214 CPU sec, 3.089 real sec
sorting block 74, #reads = 1064554 mem = 536879887 peak = 1182740480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280584, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 377, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (386.84, 168.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674790 sequences (100000282 bp) in 2.492 CPU sec, 2.702 real sec...
[M::mem_process_seqs] Processed 674578 reads in 80.749 CPU sec, 2.622 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280944, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 374, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (383.93, 168.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674630 sequences (100000138 bp) in 2.271 CPU sec, 2.417 real sec...
[M::mem_process_seqs] Processed 674790 reads in 78.599 CPU sec, 2.525 real sec
sorting block 75, #reads = 1064228 mem = 536880133 peak = 1182740480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280440, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674352 sequences (100000017 bp) in 2.160 CPU sec, 2.295 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (255, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (387.41, 170.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673514 sequences (100000088 bp) in 1.600 CPU sec, 1.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280996, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (391.77, 172.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674630 reads in 141.559 CPU sec, 4.482 real sec
[M::mem_process_seqs] Processed 674352 reads in 88.599 CPU sec, 2.782 real sec
sorting block 76, #reads = 1065422 mem = 536882143 peak = 1182740480
[M::process] read 674556 sequences (100000218 bp) in 2.077 CPU sec, 2.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280094, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 392, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (400.50, 174.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676210 sequences (100000261 bp) in 1.881 CPU sec, 1.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281196, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (391.76, 173.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673514 reads in 141.050 CPU sec, 4.472 real sec
[M::process] read 680156 sequences (100000007 bp) in 1.840 CPU sec, 1.861 real sec...
[M::mem_process_seqs] Processed 674556 reads in 90.044 CPU sec, 2.824 real sec
sorting block 77, #reads = 1062911 mem = 536876757 peak = 1182740480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281544, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 373, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (385.07, 175.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678724 sequences (100000156 bp) in 2.230 CPU sec, 2.328 real sec...
[M::process] read 678224 sequences (100000235 bp) in 1.400 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283263, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (355.50, 166.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676210 reads in 152.412 CPU sec, 4.838 real sec
sorting block 78, #reads = 1064226 mem = 536880649 peak = 1182740480
[M::mem_process_seqs] Processed 680156 reads in 98.126 CPU sec, 3.106 real sec
sorting block 79, #reads = 1063431 mem = 536881330 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282748, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 354, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (364.90, 167.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677666 sequences (100000143 bp) in 2.378 CPU sec, 2.439 real sec...
[M::process] read 676924 sequences (100000228 bp) in 1.720 CPU sec, 1.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281644, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 359, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (368.96, 168.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678724 reads in 156.769 CPU sec, 4.965 real sec
[M::mem_process_seqs] Processed 678224 reads in 98.021 CPU sec, 3.097 real sec
sorting block 80, #reads = 1063549 mem = 536879523 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282243, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 364, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (373.60, 169.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676284 sequences (100000054 bp) in 2.068 CPU sec, 2.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281867, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 369, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (377.14, 169.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675746 sequences (100000066 bp) in 1.760 CPU sec, 1.761 real sec...
[M::mem_process_seqs] Processed 677666 reads in 150.036 CPU sec, 4.757 real sec
[M::mem_process_seqs] Processed 676924 reads in 92.843 CPU sec, 2.926 real sec
sorting block 81, #reads = 1062819 mem = 536879911 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281719, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (707, 1727, 1727)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3767)
[M::mem_pestat] mean and std.dev: (1386.00, 528.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4787)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (383.59, 170.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675526 sequences (100000236 bp) in 2.503 CPU sec, 2.603 real sec...
[M::process] read 675698 sequences (100000161 bp) in 1.420 CPU sec, 1.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280972, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (387.84, 172.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676284 reads in 148.767 CPU sec, 4.711 real sec
sorting block 82, #reads = 1062691 mem = 536885454 peak = 1191985152
[M::mem_process_seqs] Processed 675746 reads in 92.918 CPU sec, 2.939 real sec
sorting block 83, #reads = 1063078 mem = 536879238 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280959, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 387, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (391.83, 172.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675486 sequences (100000242 bp) in 1.906 CPU sec, 2.017 real sec...
[M::process] read 675864 sequences (100000060 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280747, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (389.24, 172.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675526 reads in 150.224 CPU sec, 4.781 real sec
[M::mem_process_seqs] Processed 675698 reads in 94.139 CPU sec, 2.982 real sec
sorting block 84, #reads = 1062360 mem = 536877956 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 281196, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 385, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (260.45, 114.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (389.48, 171.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675722 sequences (100000121 bp) in 2.278 CPU sec, 2.384 real sec...
[M::process] read 675870 sequences (100000185 bp) in 1.440 CPU sec, 1.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281345, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 379, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (384.47, 168.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675486 reads in 155.582 CPU sec, 4.946 real sec
[M::mem_process_seqs] Processed 675864 reads in 97.441 CPU sec, 3.095 real sec
sorting block 85, #reads = 1061176 mem = 536878269 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 281597, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (273, 273, 1139)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2871)
[M::mem_pestat] mean and std.dev: (613.50, 445.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3737)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 380, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (385.16, 168.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675642 sequences (100000001 bp) in 1.990 CPU sec, 2.100 real sec...
[M::process] read 675964 sequences (100000080 bp) in 1.580 CPU sec, 1.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281166, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 379, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (384.61, 168.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675722 reads in 158.542 CPU sec, 5.042 real sec
[M::mem_process_seqs] Processed 675870 reads in 99.287 CPU sec, 3.147 real sec
sorting block 86, #reads = 1059739 mem = 536881181 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280561, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 381, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (385.93, 168.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675676 sequences (100000074 bp) in 2.434 CPU sec, 2.541 real sec...
[M::process] read 675272 sequences (100000221 bp) in 1.740 CPU sec, 1.750 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281352, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (381.62, 168.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675642 reads in 158.627 CPU sec, 5.033 real sec
sorting block 87, #reads = 1060670 mem = 536877808 peak = 1191985152
[M::mem_process_seqs] Processed 675964 reads in 98.803 CPU sec, 3.127 real sec
sorting block 88, #reads = 1060215 mem = 536885923 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280954, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (385.54, 169.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 1357, 1357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3727)
[M::mem_pestat] mean and std.dev: (769.50, 587.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4912)
[M::mem_pestat] skip orientation RR
[M::process] read 675176 sequences (100000175 bp) in 2.338 CPU sec, 2.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281008, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 379, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (384.66, 166.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675092 sequences (100000022 bp) in 1.820 CPU sec, 1.827 real sec...
[M::mem_process_seqs] Processed 675676 reads in 159.799 CPU sec, 5.066 real sec
[M::mem_process_seqs] Processed 675272 reads in 101.495 CPU sec, 3.195 real sec
sorting block 89, #reads = 1060530 mem = 536880003 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280756, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (386.60, 168.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675060 sequences (100000182 bp) in 2.646 CPU sec, 2.739 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280951, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 384, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (388.84, 168.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675248 sequences (100000135 bp) in 1.850 CPU sec, 1.851 real sec...
[M::mem_process_seqs] Processed 675176 reads in 159.568 CPU sec, 5.044 real sec
[M::mem_process_seqs] Processed 675092 reads in 99.837 CPU sec, 3.150 real sec
sorting block 90, #reads = 1059953 mem = 536878926 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280753, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (387.82, 169.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675048 sequences (100000128 bp) in 2.333 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280957, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (386.00, 168.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674966 sequences (100000262 bp) in 1.850 CPU sec, 1.846 real sec...
[M::mem_process_seqs] Processed 675060 reads in 163.231 CPU sec, 5.174 real sec
sorting block 91, #reads = 1059960 mem = 536880526 peak = 1191985152
[M::mem_process_seqs] Processed 675248 reads in 102.292 CPU sec, 3.226 real sec
sorting block 92, #reads = 1060098 mem = 536879311 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280183, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (387.29, 168.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674638 sequences (100000105 bp) in 2.662 CPU sec, 2.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280714, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (389.72, 169.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674740 sequences (100000203 bp) in 1.836 CPU sec, 1.845 real sec...
[M::mem_process_seqs] Processed 675048 reads in 162.641 CPU sec, 5.147 real sec
[M::mem_process_seqs] Processed 674966 reads in 101.152 CPU sec, 3.191 real sec
sorting block 93, #reads = 1059564 mem = 536877407 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 280687, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 308, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1333)
[M::mem_pestat] mean and std.dev: (377.27, 222.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1720)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 387, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (392.76, 170.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674488 sequences (100000277 bp) in 2.374 CPU sec, 2.446 real sec...
[M::process] read 674332 sequences (100000034 bp) in 1.410 CPU sec, 1.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281569, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 386, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (392.41, 170.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674638 reads in 155.985 CPU sec, 4.955 real sec
[M::mem_process_seqs] Processed 674740 reads in 98.775 CPU sec, 3.128 real sec
sorting block 94, #reads = 1060169 mem = 536878734 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281140, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 392, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (398.14, 174.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679144 sequences (100000076 bp) in 2.535 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280906, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 391, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (398.32, 174.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681462 sequences (100000013 bp) in 1.520 CPU sec, 1.517 real sec...
[M::mem_process_seqs] Processed 674488 reads in 152.028 CPU sec, 4.820 real sec
[M::mem_process_seqs] Processed 674332 reads in 96.140 CPU sec, 3.034 real sec
sorting block 95, #reads = 1060430 mem = 536880526 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283208, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 348, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (362.37, 168.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680024 sequences (100000224 bp) in 2.283 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283872, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (342.63, 159.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679598 sequences (100000184 bp) in 1.880 CPU sec, 1.884 real sec...
[M::mem_process_seqs] Processed 679144 reads in 162.214 CPU sec, 5.144 real sec
sorting block 96, #reads = 1061148 mem = 536879028 peak = 1191985152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283409, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (352.03, 160.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679204 sequences (100000019 bp) in 2.345 CPU sec, 2.353 real sec...
[M::mem_process_seqs] Processed 680024 reads in 90.081 CPU sec, 2.837 real sec
sorting block 97, #reads = 1060245 mem = 536879758 peak = 1194938368
[M::mem_process_seqs] Processed 681462 reads in 223.279 CPU sec, 7.057 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283664, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (354.01, 159.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679436 sequences (100000270 bp) in 2.396 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283501, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (354.83, 158.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679598 reads in 178.051 CPU sec, 5.628 real sec
[M::process] read 678276 sequences (100000274 bp) in 1.846 CPU sec, 1.854 real sec...
sorting block 98, #reads = 1059706 mem = 536878353 peak = 1194938368
[M::process] read 677910 sequences (100000074 bp) in 2.020 CPU sec, 2.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282811, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (354.11, 158.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679436 reads in 95.242 CPU sec, 3.213 real sec
sorting block 99, #reads = 1058390 mem = 536879193 peak = 1194938368
[M::mem_process_seqs] Processed 679204 reads in 226.797 CPU sec, 7.369 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283693, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (360.84, 160.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677390 sequences (100000228 bp) in 2.441 CPU sec, 2.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283034, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 356, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (363.55, 161.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678276 reads in 174.631 CPU sec, 5.532 real sec
[M::mem_process_seqs] Processed 677910 reads in 101.555 CPU sec, 3.190 real sec
[M::process] read 677348 sequences (100000104 bp) in 1.996 CPU sec, 2.041 real sec...
sorting block 100, #reads = 1058647 mem = 536879488 peak = 1194938368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283336, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 361, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (369.02, 164.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 101, #reads = 1059200 mem = 536880505 peak = 1194938368
[M::process] read 677114 sequences (100000018 bp) in 2.314 CPU sec, 2.442 real sec...
[M::process] read 676718 sequences (100000290 bp) in 1.470 CPU sec, 1.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282565, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 364, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (371.58, 165.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677390 reads in 160.868 CPU sec, 5.111 real sec
[M::mem_process_seqs] Processed 677348 reads in 102.310 CPU sec, 3.242 real sec
sorting block 102, #reads = 1059233 mem = 536882045 peak = 1194938368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282548, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 365, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (371.47, 163.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676754 sequences (100000075 bp) in 2.410 CPU sec, 2.528 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282523, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 368, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (374.02, 164.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677338 sequences (100000135 bp) in 1.840 CPU sec, 1.834 real sec...
[M::mem_process_seqs] Processed 677114 reads in 156.450 CPU sec, 4.953 real sec
[M::mem_process_seqs] Processed 676718 reads in 99.564 CPU sec, 3.134 real sec
sorting block 103, #reads = 1059032 mem = 536880946 peak = 1194938368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282195, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 368, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (374.88, 165.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676328 sequences (100000241 bp) in 2.598 CPU sec, 2.702 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282414, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 362, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (370.68, 165.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675848 sequences (100000061 bp) in 1.790 CPU sec, 1.792 real sec...
[M::mem_process_seqs] Processed 676754 reads in 158.236 CPU sec, 5.005 real sec
[M::mem_process_seqs] Processed 677338 reads in 98.910 CPU sec, 3.115 real sec
sorting block 104, #reads = 1059670 mem = 536880620 peak = 1194938368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281765, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 371, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (377.55, 166.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677182 sequences (100000278 bp) in 2.238 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281389, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 379, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (385.05, 169.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675150 sequences (100000286 bp) in 1.800 CPU sec, 1.800 real sec...
[M::mem_process_seqs] Processed 676328 reads in 153.256 CPU sec, 4.859 real sec
sorting block 105, #reads = 1059198 mem = 536883995 peak = 1194938368
[M::mem_process_seqs] Processed 675848 reads in 96.799 CPU sec, 3.051 real sec
sorting block 106, #reads = 1061758 mem = 536882462 peak = 1194938368
[M::process] read 675248 sequences (100000210 bp) in 2.441 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281324, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (391.88, 172.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674988 sequences (100000130 bp) in 1.880 CPU sec, 1.884 real sec...
[M::mem_process_seqs] Processed 675150 reads in 86.957 CPU sec, 2.729 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282504, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 362, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (374.11, 170.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281065, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 387, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (394.04, 174.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677182 reads in 243.117 CPU sec, 7.698 real sec
[M::process] read 674928 sequences (100000009 bp) in 1.992 CPU sec, 2.003 real sec...
sorting block 107, #reads = 1059869 mem = 536876935 peak = 1194938368
[M::mem_process_seqs] Processed 675248 reads in 112.368 CPU sec, 3.555 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281704, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 388, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059)
[M::mem_pestat] mean and std.dev: (396.37, 176.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 108, #reads = 1061617 mem = 536878916 peak = 1194938368
[M::process] read 675020 sequences (100000235 bp) in 2.227 CPU sec, 2.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281512, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 390, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063)
[M::mem_pestat] mean and std.dev: (397.61, 176.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1332)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 326, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (76, 581)
[M::mem_pestat] mean and std.dev: (318.50, 76.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 682)
[M::mem_pestat] skip orientation RR
[M::process] read 674646 sequences (100000174 bp) in 1.820 CPU sec, 1.819 real sec...
[M::mem_process_seqs] Processed 674988 reads in 155.446 CPU sec, 4.930 real sec
[M::mem_process_seqs] Processed 674928 reads in 98.948 CPU sec, 3.119 real sec
sorting block 109, #reads = 1061217 mem = 536880289 peak = 1194938368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281627, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (396.96, 176.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674472 sequences (100000073 bp) in 2.400 CPU sec, 2.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281304, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 390, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (398.32, 176.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674406 sequences (100000118 bp) in 1.910 CPU sec, 1.906 real sec...
[M::mem_process_seqs] Processed 675020 reads in 157.428 CPU sec, 4.980 real sec
[M::mem_process_seqs] Processed 674646 reads in 100.457 CPU sec, 3.172 real sec
sorting block 110, #reads = 1060410 mem = 536880270 peak = 1194938368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281282, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 395, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065)
[M::mem_pestat] mean and std.dev: (402.91, 179.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1333)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676078 sequences (100000139 bp) in 2.190 CPU sec, 2.289 real sec...
[M::process] read 678816 sequences (100000203 bp) in 1.580 CPU sec, 1.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281609, 0, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 393, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059)
[M::mem_pestat] mean and std.dev: (401.43, 178.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1325)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 331, 5416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15954)
[M::mem_pestat] mean and std.dev: (1479.94, 2190.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21223)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674472 reads in 161.064 CPU sec, 5.119 real sec
[M::mem_process_seqs] Processed 674406 reads in 101.996 CPU sec, 3.228 real sec
sorting block 111, #reads = 1058907 mem = 536881137 peak = 1194938368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282130, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 378, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (387.78, 175.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678168 sequences (100000268 bp) in 2.636 CPU sec, 2.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282975, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 350, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (364.05, 169.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677562 sequences (100000285 bp) in 1.900 CPU sec, 1.897 real sec...
[M::mem_process_seqs] Processed 676078 reads in 168.905 CPU sec, 5.347 real sec
sorting block 112, #reads = 1058172 mem = 536879440 peak = 1194938368
[M::mem_process_seqs] Processed 678816 reads in 101.052 CPU sec, 3.194 real sec
sorting block 113, #reads = 1054843 mem = 536886971 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282940, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 357, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (369.76, 170.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677432 sequences (100000070 bp) in 2.000 CPU sec, 2.093 real sec...
[M::process] read 676570 sequences (100000162 bp) in 1.500 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282029, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 359, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (370.27, 167.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678168 reads in 151.988 CPU sec, 4.837 real sec
[M::mem_process_seqs] Processed 677562 reads in 95.579 CPU sec, 3.022 real sec
sorting block 114, #reads = 1063904 mem = 536878587 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282202, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 365, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (374.17, 169.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676364 sequences (100000200 bp) in 2.440 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281438, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 369, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (378.10, 169.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676442 sequences (100000157 bp) in 1.850 CPU sec, 1.848 real sec...
[M::mem_process_seqs] Processed 677432 reads in 153.730 CPU sec, 4.865 real sec
[M::mem_process_seqs] Processed 676570 reads in 96.344 CPU sec, 3.034 real sec
sorting block 115, #reads = 1062571 mem = 536879360 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281596, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 372, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (381.75, 171.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676044 sequences (100000113 bp) in 2.062 CPU sec, 2.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282190, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 376, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (384.60, 173.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675930 sequences (100000102 bp) in 1.790 CPU sec, 1.792 real sec...
[M::mem_process_seqs] Processed 676364 reads in 148.033 CPU sec, 4.697 real sec
sorting block 116, #reads = 1063361 mem = 536878593 peak = 1197727744
[M::mem_process_seqs] Processed 676442 reads in 91.935 CPU sec, 2.898 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282596, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (386.31, 172.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 117, #reads = 1064317 mem = 536879366 peak = 1197727744
[M::process] read 676018 sequences (100000229 bp) in 2.586 CPU sec, 2.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281730, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (386.65, 173.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676044 reads in 146.790 CPU sec, 4.649 real sec
[M::process] read 675736 sequences (100000029 bp) in 1.880 CPU sec, 1.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281895, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 248, 736)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870)
[M::mem_pestat] mean and std.dev: (354.90, 251.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2437)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (385.72, 171.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676018 reads in 79.201 CPU sec, 2.502 real sec
[M::process] read 676234 sequences (100000166 bp) in 1.946 CPU sec, 1.956 real sec...
sorting block 118, #reads = 1064122 mem = 536880077 peak = 1197727744
[M::mem_process_seqs] Processed 675930 reads in 190.418 CPU sec, 6.006 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281878, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (384.62, 171.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675512 sequences (100000001 bp) in 2.625 CPU sec, 2.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281928, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 374, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (382.26, 170.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675736 reads in 159.871 CPU sec, 5.051 real sec
sorting block 119, #reads = 1063197 mem = 536886204 peak = 1197727744
[M::mem_process_seqs] Processed 676234 reads in 93.863 CPU sec, 2.948 real sec
[M::process] read 675372 sequences (100000239 bp) in 1.960 CPU sec, 2.029 real sec...
sorting block 120, #reads = 1061624 mem = 536879361 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281434, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (387.84, 172.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675194 sequences (100000168 bp) in 2.512 CPU sec, 2.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281002, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 378, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (385.84, 169.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675512 reads in 151.512 CPU sec, 4.802 real sec
[M::process] read 675246 sequences (100000131 bp) in 1.833 CPU sec, 1.836 real sec...
[M::mem_process_seqs] Processed 675372 reads in 95.075 CPU sec, 2.985 real sec
sorting block 121, #reads = 1061596 mem = 536879236 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281055, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (390.88, 172.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675172 sequences (100000289 bp) in 2.370 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281039, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (389.98, 172.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 180, 239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 385)
[M::mem_pestat] mean and std.dev: (198.64, 42.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 458)
[M::mem_pestat] skip orientation RR
[M::process] read 674912 sequences (100000042 bp) in 1.500 CPU sec, 1.500 real sec...
[M::mem_process_seqs] Processed 675194 reads in 149.931 CPU sec, 4.759 real sec
[M::mem_process_seqs] Processed 675246 reads in 95.445 CPU sec, 3.013 real sec
sorting block 122, #reads = 1061781 mem = 536877909 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280550, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (393.56, 174.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674996 sequences (100000106 bp) in 2.191 CPU sec, 2.296 real sec...
[M::process] read 674954 sequences (100000187 bp) in 1.400 CPU sec, 1.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281289, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (393.92, 173.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675172 reads in 151.908 CPU sec, 4.826 real sec
sorting block 123, #reads = 1061600 mem = 536882987 peak = 1197727744
[M::mem_process_seqs] Processed 674912 reads in 95.710 CPU sec, 3.040 real sec
sorting block 124, #reads = 1061279 mem = 536879835 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281625, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (390.53, 172.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674968 sequences (100000198 bp) in 2.639 CPU sec, 2.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280643, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (987, 1122, 1122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (717, 1392)
[M::mem_pestat] mean and std.dev: (1083.43, 60.99)
[M::mem_pestat] low and high boundaries for proper pairs: (582, 1527)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 386, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (394.77, 175.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674996 reads in 153.392 CPU sec, 4.852 real sec
[M::process] read 675016 sequences (100000226 bp) in 1.869 CPU sec, 1.867 real sec...
[M::mem_process_seqs] Processed 674954 reads in 97.720 CPU sec, 3.070 real sec
sorting block 125, #reads = 1062175 mem = 536880176 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281186, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (390.18, 172.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674802 sequences (100000265 bp) in 2.278 CPU sec, 2.396 real sec...
[M::process] read 675016 sequences (100000055 bp) in 1.410 CPU sec, 1.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280799, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (393.26, 174.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 417, 602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1432)
[M::mem_pestat] mean and std.dev: (374.62, 166.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1847)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674968 reads in 151.801 CPU sec, 4.823 real sec
[M::mem_process_seqs] Processed 675016 reads in 94.812 CPU sec, 3.003 real sec
sorting block 126, #reads = 1060920 mem = 536878659 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281676, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (393.92, 175.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675272 sequences (100000225 bp) in 2.314 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281832, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (393.81, 175.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674800 sequences (100000139 bp) in 1.850 CPU sec, 1.846 real sec...
[M::mem_process_seqs] Processed 674802 reads in 152.735 CPU sec, 4.833 real sec
[M::mem_process_seqs] Processed 675016 reads in 96.731 CPU sec, 3.047 real sec
sorting block 127, #reads = 1061013 mem = 536877163 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281473, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (393.63, 176.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674866 sequences (100000156 bp) in 2.621 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281359, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 386, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (396.40, 177.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1316)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675272 reads in 151.286 CPU sec, 4.784 real sec
[M::process] read 674724 sequences (100000298 bp) in 1.829 CPU sec, 1.827 real sec...
sorting block 128, #reads = 1060350 mem = 536878892 peak = 1197727744
[M::mem_process_seqs] Processed 674800 reads in 95.440 CPU sec, 3.003 real sec
sorting block 129, #reads = 1060023 mem = 536879137 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281564, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (395.46, 177.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681150 sequences (100000059 bp) in 2.299 CPU sec, 2.377 real sec...
[M::process] read 681464 sequences (100000233 bp) in 1.460 CPU sec, 1.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281089, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 390, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062)
[M::mem_pestat] mean and std.dev: (400.83, 181.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1330)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674866 reads in 157.858 CPU sec, 5.015 real sec
[M::mem_process_seqs] Processed 674724 reads in 103.310 CPU sec, 3.273 real sec
sorting block 130, #reads = 1057893 mem = 536880572 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283664, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 331, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (348.68, 165.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679918 sequences (100000151 bp) in 2.760 CPU sec, 2.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284141, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 328, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (343.32, 159.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679090 sequences (100000028 bp) in 1.830 CPU sec, 1.831 real sec...
[M::mem_process_seqs] Processed 681150 reads in 164.666 CPU sec, 5.209 real sec
[M::mem_process_seqs] Processed 681464 reads in 101.753 CPU sec, 3.201 real sec
sorting block 131, #reads = 1052017 mem = 536879105 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283633, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (351.50, 160.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678820 sequences (100000269 bp) in 2.459 CPU sec, 2.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283786, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (354.40, 159.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 339, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (337.40, 121.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RR
[M::process] read 678144 sequences (100000038 bp) in 1.870 CPU sec, 1.867 real sec...
[M::mem_process_seqs] Processed 679918 reads in 155.691 CPU sec, 4.930 real sec
sorting block 132, #reads = 1059887 mem = 536880021 peak = 1197727744
[M::mem_process_seqs] Processed 679090 reads in 96.290 CPU sec, 3.036 real sec
sorting block 133, #reads = 1060100 mem = 536877032 peak = 1197727744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283265, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (357.67, 159.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677948 sequences (100000147 bp) in 2.650 CPU sec, 2.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282459, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 272, 272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 452)
[M::mem_pestat] mean and std.dev: (232.89, 49.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 542)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 356, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (364.57, 163.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677432 sequences (100000269 bp) in 1.790 CPU sec, 1.797 real sec...
[M::mem_process_seqs] Processed 678820 reads in 158.286 CPU sec, 5.009 real sec
[M::mem_process_seqs] Processed 678144 reads in 102.975 CPU sec, 3.242 real sec
sorting block 134, #reads = 1058654 mem = 536879232 peak = 1197727744
[M::process] read 676846 sequences (100000094 bp) in 2.428 CPU sec, 2.517 real sec...
[M::process] read 677158 sequences (100000276 bp) in 1.440 CPU sec, 1.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282710, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 363, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (370.64, 165.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677432 reads in 86.765 CPU sec, 2.746 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282686, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 359, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (367.69, 165.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282219, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 367, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (375.08, 167.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677948 reads in 235.993 CPU sec, 7.487 real sec
[M::process] read 677206 sequences (100000118 bp) in 1.855 CPU sec, 1.858 real sec...
sorting block 135, #reads = 1057777 mem = 536879812 peak = 1197727744
[M::mem_process_seqs] Processed 676846 reads in 110.349 CPU sec, 3.509 real sec
sorting block 136, #reads = 1058463 mem = 536879429 peak = 1225768960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282148, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 363, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (372.07, 165.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676074 sequences (100000007 bp) in 2.388 CPU sec, 2.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282387, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 359, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (366.80, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675290 sequences (100000240 bp) in 1.800 CPU sec, 1.802 real sec...
[M::mem_process_seqs] Processed 677158 reads in 155.738 CPU sec, 4.935 real sec
[M::mem_process_seqs] Processed 677206 reads in 99.449 CPU sec, 3.134 real sec
sorting block 137, #reads = 1059141 mem = 536878724 peak = 1225768960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282005, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 370, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (378.04, 166.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676030 sequences (100000113 bp) in 2.417 CPU sec, 2.549 real sec...
[M::process] read 676166 sequences (100000116 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281590, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 378, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (383.76, 166.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676074 reads in 149.841 CPU sec, 4.746 real sec
[M::mem_process_seqs] Processed 675290 reads in 93.548 CPU sec, 2.961 real sec
sorting block 138, #reads = 1059238 mem = 536881984 peak = 1225768960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281897, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 368, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (376.00, 164.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676114 sequences (100000167 bp) in 2.386 CPU sec, 2.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281827, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 369, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (376.13, 165.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675636 sequences (100000180 bp) in 1.790 CPU sec, 1.789 real sec...
[M::mem_process_seqs] Processed 676030 reads in 151.365 CPU sec, 4.792 real sec
sorting block 139, #reads = 1059339 mem = 536878675 peak = 1225768960
[M::mem_process_seqs] Processed 676166 reads in 94.079 CPU sec, 2.957 real sec
sorting block 140, #reads = 1059196 mem = 536877825 peak = 1225768960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281479, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 372, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (379.21, 166.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675504 sequences (100000131 bp) in 2.578 CPU sec, 2.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280969, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 373, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (380.89, 166.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676114 reads in 148.822 CPU sec, 4.708 real sec
[M::process] read 675786 sequences (100000093 bp) in 1.836 CPU sec, 1.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281477, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (382.28, 167.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675294 sequences (100000150 bp) in 2.000 CPU sec, 2.007 real sec...
[M::mem_process_seqs] Processed 675504 reads in 85.935 CPU sec, 2.898 real sec
sorting block 141, #reads = 1060463 mem = 536878254 peak = 1225768960
[M::mem_process_seqs] Processed 675636 reads in 197.686 CPU sec, 6.426 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280698, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 374, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (382.71, 169.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 142, #reads = 1061046 mem = 536878569 peak = 1225768960
[M::process] read 675392 sequences (100000269 bp) in 2.447 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280965, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (387.95, 171.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675786 reads in 162.426 CPU sec, 5.137 real sec
[M::mem_process_seqs] Processed 675294 reads in 94.156 CPU sec, 2.952 real sec
[M::process] read 675334 sequences (100000139 bp) in 1.982 CPU sec, 2.015 real sec...
sorting block 143, #reads = 1061437 mem = 536886607 peak = 1225768960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280873, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 379, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (388.09, 172.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674900 sequences (100000052 bp) in 2.237 CPU sec, 2.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281066, 6, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 533)
[M::mem_pestat] mean and std.dev: (266.93, 80.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (388.79, 172.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675392 reads in 147.260 CPU sec, 4.669 real sec
[M::process] read 673990 sequences (100000025 bp) in 1.850 CPU sec, 1.860 real sec...
[M::mem_process_seqs] Processed 675334 reads in 92.967 CPU sec, 2.920 real sec
sorting block 144, #reads = 1060352 mem = 536880676 peak = 1225768960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281269, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (392.46, 174.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674814 sequences (100000252 bp) in 2.535 CPU sec, 2.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280658, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 398, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (404.02, 176.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1325)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674736 sequences (100000185 bp) in 1.800 CPU sec, 1.794 real sec...
[M::mem_process_seqs] Processed 674900 reads in 152.542 CPU sec, 4.825 real sec
[M::mem_process_seqs] Processed 673990 reads in 95.715 CPU sec, 3.013 real sec
sorting block 145, #reads = 1059912 mem = 536878727 peak = 1225768960
[M::process] read 82280 sequences (12171459 bp) in 0.355 CPU sec, 0.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280826, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 387, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (396.35, 176.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281158, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (394.79, 175.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 34326, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 371, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (381.30, 171.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674814 reads in 158.031 CPU sec, 5.114 real sec
sorting block 146, #reads = 1060976 mem = 536877759 peak = 1225768960
[M::mem_process_seqs] Processed 674736 reads in 105.423 CPU sec, 3.400 real sec
[M::mem_process_seqs] Processed 82280 reads in 107.482 CPU sec, 3.471 real sec
sorting block 147, #reads = 1059341 mem = 536878311 peak = 1279488000
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 156822434
overall: 69926105088 mem 20469.372 user 202.468 sys 668.548 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.L007\tPU:L007\tSM:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tLB:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R1.fastq.gz FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 668.579 sec; CPU: 20671.842 sec
sorting block 148, #reads = 235251 mem = 119617460 peak = 1279488000
uniq header count 1
merging 149 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 64
algo: util-sort
license: sentieon:util=1
output file size: 9056925924
output reads: 157530891
bam_mem_sort: 149 calls 78.250 user 1.182 sys 79.801 real
bam_write: 149 calls 41.383 user 2.504 sys 47.373 real
execute: 1 calls 42.841 user 0.824 sys 730.463 real
merge_files: 1 calls 42.822 user 0.797 sys 61.773 real
parse_chunk: 2372 calls 92.413 user 26.517 sys 120.950 real
read_chunk: 6400 calls 53.002 user 41.839 sys 639.580 real
sort_block: 1 calls 33.274 user 41.328 sys 612.482 real
write_chunk: 4026 calls 42.443 user 0.566 sys 60.921 real
overall: 3297259520 mem 2355.404 user 86.700 sys 730.478 real
ls: cannot access '*.cram': No such file or directory