cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_6TL-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2871.241 CPU sec, 116.218 real sec [M::process] read 699530 sequences (100000284 bp) in 1.242 CPU sec, 1.251 real sec... [M::process] read 696602 sequences (100000181 bp) in 1.949 CPU sec, 1.953 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (103, 288130, 30, 98) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 348, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2969) [M::mem_pestat] mean and std.dev: (662.97, 646.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (272.31, 137.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 697, 905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2455) [M::mem_pestat] mean and std.dev: (567.85, 576.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 298, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (330.32, 175.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698636 sequences (100000088 bp) in 2.244 CPU sec, 2.350 real sec... [M::process] read 700338 sequences (100000075 bp) in 1.766 CPU sec, 1.858 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 287735, 33, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 278, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (336.31, 221.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (282.37, 142.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1000) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 1093, 1951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5365) [M::mem_pestat] mean and std.dev: (1084.75, 920.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 344, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213) [M::mem_pestat] mean and std.dev: (339.27, 161.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699530 reads in 231.462 CPU sec, 7.644 real sec [M::mem_process_seqs] Processed 696602 reads in 127.622 CPU sec, 4.254 real sec sorting block 0, #reads = 1084949 mem = 536881274 peak = 569262080 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288655, 32, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 264, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (291.97, 169.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 252, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (277.53, 140.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (229, 417, 5333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15541) [M::mem_pestat] mean and std.dev: (2162.50, 2765.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 328, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1890) [M::mem_pestat] mean and std.dev: (460.24, 422.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700690 sequences (100000064 bp) in 3.095 CPU sec, 3.380 real sec... [M::process] read 697356 sequences (100000231 bp) in 1.946 CPU sec, 1.961 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 289001, 23, 87) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 252, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456) [M::mem_pestat] mean and std.dev: (367.58, 313.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (271.37, 138.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (448, 646, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2083) [M::mem_pestat] mean and std.dev: (860.22, 635.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3401) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 284, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (273.62, 114.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698636 reads in 194.905 CPU sec, 6.217 real sec [M::mem_process_seqs] Processed 700338 reads in 130.470 CPU sec, 4.156 real sec sorting block 1, #reads = 1086125 mem = 536878492 peak = 698236928 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 289091, 20, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 239, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265) [M::mem_pestat] mean and std.dev: (288.35, 186.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (269.85, 137.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 511, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (434.56, 432.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (310.97, 179.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698752 sequences (100000283 bp) in 3.070 CPU sec, 3.299 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 288339, 19, 93) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 270, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1965) [M::mem_pestat] mean and std.dev: (338.62, 278.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 258, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (282.71, 143.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1042, 1042, 1995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3901) [M::mem_pestat] mean and std.dev: (1379.37, 919.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5058) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 286, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (262.88, 89.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700142 sequences (100000198 bp) in 1.978 CPU sec, 1.998 real sec... [M::mem_process_seqs] Processed 700690 reads in 197.010 CPU sec, 6.295 real sec [M::mem_process_seqs] Processed 697356 reads in 130.530 CPU sec, 4.162 real sec sorting block 2, #reads = 1086673 mem = 536882915 peak = 716771328 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 288230, 31, 118) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 316, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2717) [M::mem_pestat] mean and std.dev: (429.66, 400.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (275.40, 140.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (457, 713, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1507) [M::mem_pestat] mean and std.dev: (607.96, 260.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 281, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115) [M::mem_pestat] mean and std.dev: (315.58, 180.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699308 sequences (100000147 bp) in 3.280 CPU sec, 3.854 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 289136, 18, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 239, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (302.06, 184.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (272.37, 140.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (346, 701, 2329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6295) [M::mem_pestat] mean and std.dev: (1441.06, 1461.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 411, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (363.21, 192.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698752 reads in 185.001 CPU sec, 5.941 real sec [M::mem_process_seqs] Processed 700142 reads in 130.553 CPU sec, 4.184 real sec sorting block 3, #reads = 1086635 mem = 536881803 peak = 716771328 [M::process] read 698976 sequences (100000131 bp) in 2.370 CPU sec, 2.511 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 288190, 20, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 451, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (460.18, 263.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (273.03, 139.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 335, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3435) [M::mem_pestat] mean and std.dev: (659.58, 674.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 275, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2152) [M::mem_pestat] mean and std.dev: (446.10, 375.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2802) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 4, #reads = 1085570 mem = 536882689 peak = 796925952 [M::process] read 695956 sequences (100000242 bp) in 3.446 CPU sec, 4.002 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288098, 21, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 308, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3008) [M::mem_pestat] mean and std.dev: (565.14, 483.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (271.52, 137.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 1287, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3564) [M::mem_pestat] mean and std.dev: (1197.33, 679.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 303, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (333.89, 185.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699308 reads in 208.610 CPU sec, 6.792 real sec [M::mem_process_seqs] Processed 698976 reads in 138.601 CPU sec, 4.455 real sec [M::process] read 699218 sequences (100000225 bp) in 3.652 CPU sec, 3.976 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 287269, 21, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 293, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2503) [M::mem_pestat] mean and std.dev: (453.46, 420.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (279.87, 140.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (429, 649, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2760) [M::mem_pestat] mean and std.dev: (723.10, 491.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3537) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 342, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (403.52, 289.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 5, #reads = 1086026 mem = 536885062 peak = 796925952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 288211, 24, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 263, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (294.98, 168.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (269.38, 135.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (815, 1129, 2327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5351) [M::mem_pestat] mean and std.dev: (1339.82, 1021.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6863) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 250, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1887) [M::mem_pestat] mean and std.dev: (426.07, 433.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696714 sequences (100000110 bp) in 3.271 CPU sec, 3.619 real sec... [M::mem_process_seqs] Processed 695956 reads in 211.583 CPU sec, 6.892 real sec [M::mem_process_seqs] Processed 699218 reads in 141.315 CPU sec, 4.625 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 287040, 28, 99) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 246, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008) [M::mem_pestat] mean and std.dev: (264.88, 154.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (278.31, 139.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (489, 1690, 3259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8799) [M::mem_pestat] mean and std.dev: (1960.11, 1613.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 267, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (276.11, 118.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696714 reads in 118.930 CPU sec, 3.995 real sec [M::process] read 698172 sequences (100000281 bp) in 3.756 CPU sec, 4.207 real sec... sorting block 6, #reads = 1084793 mem = 536885558 peak = 796925952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (94, 287662, 30, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 252, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (336.82, 252.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (272.10, 136.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (132, 1463, 4147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12177) [M::mem_pestat] mean and std.dev: (2290.13, 2308.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 304, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (308.00, 148.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697652 sequences (100000065 bp) in 3.442 CPU sec, 3.814 real sec... [M::mem_process_seqs] Processed 698172 reads in 116.325 CPU sec, 3.978 real sec sorting block 7, #reads = 1085286 mem = 536877207 peak = 796925952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 287677, 26, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 268, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2415) [M::mem_pestat] mean and std.dev: (615.04, 668.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (270.96, 134.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (137, 517, 1819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5183) [M::mem_pestat] mean and std.dev: (1216.08, 1454.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7033) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 278, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (276.70, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1329) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 695214 sequences (100000031 bp) in 3.555 CPU sec, 3.872 real sec... [M::mem_process_seqs] Processed 697652 reads in 115.493 CPU sec, 4.310 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 286572, 28, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 185, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (206.12, 116.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (279.92, 138.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 433, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1740) [M::mem_pestat] mean and std.dev: (409.96, 302.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 295, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761) [M::mem_pestat] mean and std.dev: (428.27, 304.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697668 sequences (100000152 bp) in 3.522 CPU sec, 3.859 real sec... [M::mem_process_seqs] Processed 695214 reads in 114.279 CPU sec, 3.915 real sec sorting block 8, #reads = 1083948 mem = 536878336 peak = 796925952 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 287575, 20, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 275, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (233.80, 110.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (271.36, 134.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 1089, 2034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5504) [M::mem_pestat] mean and std.dev: (1048.50, 757.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 285, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (313.91, 200.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697668 reads in 115.316 CPU sec, 3.901 real sec [M::process] read 698154 sequences (100000047 bp) in 3.592 CPU sec, 3.988 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 288046, 32, 103) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 253, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (277.78, 195.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (270.07, 134.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 439, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2481) [M::mem_pestat] mean and std.dev: (561.80, 451.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 337, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1687) [M::mem_pestat] mean and std.dev: (437.32, 393.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2180) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 9, #reads = 1082509 mem = 536885678 peak = 796925952 [M::mem_process_seqs] Processed 698154 reads in 109.969 CPU sec, 3.723 real sec [M::process] read 696340 sequences (100000111 bp) in 3.624 CPU sec, 4.094 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 287164, 20, 99) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 210, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (223.27, 115.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.41, 137.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (648, 1040, 1990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4674) [M::mem_pestat] mean and std.dev: (1074.67, 648.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6016) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 335, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2082) [M::mem_pestat] mean and std.dev: (468.46, 380.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697788 sequences (100000113 bp) in 3.217 CPU sec, 3.483 real sec... [M::mem_process_seqs] Processed 696340 reads in 110.252 CPU sec, 3.720 real sec sorting block 10, #reads = 1084119 mem = 536879593 peak = 796925952 [M::process] read 696752 sequences (100000121 bp) in 2.589 CPU sec, 2.785 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 287745, 20, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 267, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (265.97, 120.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (273.49, 137.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (523, 1353, 2444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6286) [M::mem_pestat] mean and std.dev: (1647.25, 1497.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 373, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2852) [M::mem_pestat] mean and std.dev: (584.42, 542.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697788 reads in 112.246 CPU sec, 3.781 real sec sorting block 11, #reads = 1083912 mem = 536878421 peak = 796925952 [M::process] read 698210 sequences (100000174 bp) in 2.849 CPU sec, 3.074 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 287407, 21, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 298, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1182) [M::mem_pestat] mean and std.dev: (318.70, 221.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 253, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (277.58, 139.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 829, 2170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5298) [M::mem_pestat] mean and std.dev: (1208.65, 976.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 310, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157) [M::mem_pestat] mean and std.dev: (332.93, 206.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1470) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699412 sequences (100000272 bp) in 2.387 CPU sec, 2.563 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 287655, 41, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 269, 947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2451) [M::mem_pestat] mean and std.dev: (530.86, 561.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (274.77, 138.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (705, 825, 1162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2076) [M::mem_pestat] mean and std.dev: (829.41, 401.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 295, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1680) [M::mem_pestat] mean and std.dev: (334.21, 239.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2183) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696752 reads in 201.594 CPU sec, 6.485 real sec sorting block 12, #reads = 1084502 mem = 536882632 peak = 796925952 [M::mem_process_seqs] Processed 698210 reads in 136.576 CPU sec, 4.348 real sec [M::process] read 698110 sequences (100000228 bp) in 2.037 CPU sec, 2.092 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 288185, 21, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 303, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (301.85, 165.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (272.13, 137.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (322, 720, 1888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5020) [M::mem_pestat] mean and std.dev: (1138.76, 1109.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 391, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2402) [M::mem_pestat] mean and std.dev: (622.82, 541.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 13, #reads = 1083427 mem = 536876769 peak = 799211520 [M::process] read 699308 sequences (100000205 bp) in 3.070 CPU sec, 3.426 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 288102, 34, 96) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 249, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1886) [M::mem_pestat] mean and std.dev: (421.80, 454.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (278.11, 140.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 617, 2128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6292) [M::mem_pestat] mean and std.dev: (736.86, 888.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 276, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (279.90, 137.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699412 reads in 195.464 CPU sec, 6.298 real sec [M::mem_process_seqs] Processed 698110 reads in 131.928 CPU sec, 4.181 real sec [M::process] read 698668 sequences (100000195 bp) in 2.287 CPU sec, 2.381 real sec... sorting block 14, #reads = 1086281 mem = 536879418 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 288256, 30, 98) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 250, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (267.45, 128.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (274.73, 139.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (494, 1453, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4226) [M::mem_pestat] mean and std.dev: (1134.00, 694.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 279, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (334.54, 215.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699464 sequences (100000233 bp) in 2.764 CPU sec, 3.074 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 288460, 19, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 280, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1545) [M::mem_pestat] mean and std.dev: (351.35, 269.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 251, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (276.01, 139.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 153, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2467) [M::mem_pestat] mean and std.dev: (422.00, 487.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 342, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184) [M::mem_pestat] mean and std.dev: (385.30, 220.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699308 reads in 197.043 CPU sec, 6.338 real sec [M::mem_process_seqs] Processed 698668 reads in 132.144 CPU sec, 4.207 real sec [M::process] read 697058 sequences (100000225 bp) in 2.634 CPU sec, 2.825 real sec... sorting block 15, #reads = 1085767 mem = 536878021 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 287773, 18, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 255, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (273.53, 189.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (272.88, 138.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (474, 675, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4548) [M::mem_pestat] mean and std.dev: (968.76, 785.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5906) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 350, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024) [M::mem_pestat] mean and std.dev: (440.78, 385.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699480 sequences (100000164 bp) in 2.809 CPU sec, 3.019 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 287797, 20, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 222, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (284.54, 186.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (280.87, 141.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 761, 2336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6214) [M::mem_pestat] mean and std.dev: (1481.00, 1615.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8153) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 333, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1390) [M::mem_pestat] mean and std.dev: (324.18, 201.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1783) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699464 reads in 191.907 CPU sec, 6.138 real sec sorting block 16, #reads = 1085925 mem = 536879746 peak = 799211520 [M::process] read 698824 sequences (100000149 bp) in 2.212 CPU sec, 2.257 real sec... [M::mem_process_seqs] Processed 697058 reads in 131.164 CPU sec, 4.157 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 288023, 19, 102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 377, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959) [M::mem_pestat] mean and std.dev: (496.49, 441.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (272.86, 138.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 672, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2999) [M::mem_pestat] mean and std.dev: (727.32, 556.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (278.64, 181.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 17, #reads = 1086010 mem = 536879574 peak = 799211520 [M::process] read 698828 sequences (100000115 bp) in 2.719 CPU sec, 2.979 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (114, 287995, 28, 120) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 245, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (240.65, 130.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (273.29, 138.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (391, 894, 2242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5944) [M::mem_pestat] mean and std.dev: (1166.75, 1212.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7795) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 1015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2631) [M::mem_pestat] mean and std.dev: (555.08, 510.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3439) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696574 sequences (100000262 bp) in 2.053 CPU sec, 2.055 real sec... [M::mem_process_seqs] Processed 699480 reads in 197.434 CPU sec, 6.319 real sec [M::mem_process_seqs] Processed 698824 reads in 133.241 CPU sec, 4.228 real sec sorting block 18, #reads = 1086574 mem = 536878856 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 288336, 26, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 239, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (228.12, 89.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (274.58, 139.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (358, 629, 1400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3484) [M::mem_pestat] mean and std.dev: (731.68, 552.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 295, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (335.99, 221.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696168 sequences (100000014 bp) in 2.560 CPU sec, 2.775 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 287253, 17, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 238, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2168) [M::mem_pestat] mean and std.dev: (426.05, 383.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (278.92, 139.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 402, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2897) [M::mem_pestat] mean and std.dev: (503.18, 373.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 309, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394) [M::mem_pestat] mean and std.dev: (323.86, 215.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698122 sequences (100000015 bp) in 2.036 CPU sec, 2.038 real sec... [M::mem_process_seqs] Processed 698828 reads in 199.789 CPU sec, 6.663 real sec [M::mem_process_seqs] Processed 696574 reads in 129.463 CPU sec, 4.116 real sec sorting block 19, #reads = 1085901 mem = 536880116 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (106, 287522, 10, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 313, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (320.32, 208.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (279.36, 139.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (411, 849, 1327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3159) [M::mem_pestat] mean and std.dev: (760.33, 402.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 311, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085) [M::mem_pestat] mean and std.dev: (308.40, 181.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698446 sequences (100000201 bp) in 2.910 CPU sec, 3.222 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 287811, 22, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 301, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (290.22, 148.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (272.14, 136.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 554, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3286) [M::mem_pestat] mean and std.dev: (612.79, 594.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 229, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (259.55, 158.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696168 reads in 196.725 CPU sec, 6.294 real sec [M::process] read 698406 sequences (100000005 bp) in 2.080 CPU sec, 2.090 real sec... sorting block 20, #reads = 1084190 mem = 536881381 peak = 799211520 [M::mem_process_seqs] Processed 698122 reads in 134.106 CPU sec, 4.247 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 288306, 34, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (252.18, 147.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (273.14, 138.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 670, 1684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4892) [M::mem_pestat] mean and std.dev: (865.29, 917.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 254, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (314.10, 209.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697208 sequences (100000060 bp) in 3.124 CPU sec, 3.596 real sec... sorting block 21, #reads = 1085081 mem = 536882658 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 288375, 21, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 246, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240) [M::mem_pestat] mean and std.dev: (318.83, 233.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (271.46, 136.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 688, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2625) [M::mem_pestat] mean and std.dev: (601.16, 386.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 377, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (382.32, 229.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696662 sequences (100000032 bp) in 1.950 CPU sec, 1.953 real sec... [M::mem_process_seqs] Processed 698446 reads in 196.511 CPU sec, 6.342 real sec [M::mem_process_seqs] Processed 698406 reads in 131.594 CPU sec, 4.255 real sec sorting block 22, #reads = 1086181 mem = 536880971 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 287661, 26, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 261, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1535) [M::mem_pestat] mean and std.dev: (345.03, 289.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.78, 136.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (446, 953, 2303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6017) [M::mem_pestat] mean and std.dev: (1246.27, 1094.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7874) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 294, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (345.56, 227.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698444 sequences (100000249 bp) in 2.700 CPU sec, 2.907 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (96, 287498, 14, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 200, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179) [M::mem_pestat] mean and std.dev: (268.05, 191.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.35, 137.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (365, 546, 1822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4736) [M::mem_pestat] mean and std.dev: (891.14, 750.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 288, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (275.69, 111.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698404 sequences (100000070 bp) in 2.070 CPU sec, 2.067 real sec... [M::mem_process_seqs] Processed 697208 reads in 193.601 CPU sec, 6.169 real sec [M::mem_process_seqs] Processed 696662 reads in 129.490 CPU sec, 4.108 real sec sorting block 23, #reads = 1085527 mem = 536882654 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 288097, 17, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 264, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (292.23, 164.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (270.67, 135.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (390, 810, 1845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4755) [M::mem_pestat] mean and std.dev: (1064.31, 1041.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6210) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 326, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038) [M::mem_pestat] mean and std.dev: (352.49, 200.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698296 sequences (100000203 bp) in 3.261 CPU sec, 3.475 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 288056, 20, 103) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 323, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2439) [M::mem_pestat] mean and std.dev: (471.57, 402.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (270.77, 135.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (171, 662, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4125) [M::mem_pestat] mean and std.dev: (789.00, 797.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 277, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (293.00, 139.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698588 sequences (100000241 bp) in 2.177 CPU sec, 2.185 real sec... [M::mem_process_seqs] Processed 698444 reads in 196.451 CPU sec, 6.253 real sec [M::mem_process_seqs] Processed 698404 reads in 132.151 CPU sec, 4.187 real sec sorting block 24, #reads = 1084154 mem = 536879152 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 287981, 33, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 254, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (240.79, 109.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (272.07, 136.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (690, 1589, 3779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9957) [M::mem_pestat] mean and std.dev: (2164.09, 2053.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13046) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 342, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1541) [M::mem_pestat] mean and std.dev: (414.48, 293.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699518 sequences (100000253 bp) in 3.267 CPU sec, 3.704 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 288365, 34, 87) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 268, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749) [M::mem_pestat] mean and std.dev: (480.99, 540.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (272.31, 137.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (395, 524, 1040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2330) [M::mem_pestat] mean and std.dev: (555.30, 316.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2975) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 297, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1302) [M::mem_pestat] mean and std.dev: (324.92, 198.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698296 reads in 200.687 CPU sec, 6.425 real sec sorting block 25, #reads = 1084784 mem = 536880263 peak = 799211520 [M::process] read 700122 sequences (100000059 bp) in 2.395 CPU sec, 2.441 real sec... [M::mem_process_seqs] Processed 698588 reads in 134.913 CPU sec, 4.273 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 288569, 31, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 242, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486) [M::mem_pestat] mean and std.dev: (402.16, 360.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1920) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (270.64, 136.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 958, 2726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7698) [M::mem_pestat] mean and std.dev: (1317.53, 1355.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 268, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (343.22, 198.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 26, #reads = 1084026 mem = 536877151 peak = 799211520 [M::process] read 700228 sequences (100000195 bp) in 3.195 CPU sec, 3.411 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 288536, 21, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 312, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (293.49, 157.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 246, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (269.59, 134.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (732, 1008, 4273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11355) [M::mem_pestat] mean and std.dev: (1893.00, 1616.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 361, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772) [M::mem_pestat] mean and std.dev: (433.13, 310.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699178 sequences (100000091 bp) in 1.920 CPU sec, 1.936 real sec... [M::mem_process_seqs] Processed 699518 reads in 201.314 CPU sec, 6.435 real sec [M::mem_process_seqs] Processed 700122 reads in 142.312 CPU sec, 4.560 real sec sorting block 27, #reads = 1083568 mem = 536878469 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 288691, 40, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 335, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1369) [M::mem_pestat] mean and std.dev: (380.20, 251.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (269.30, 135.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (679, 1127, 2855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7207) [M::mem_pestat] mean and std.dev: (1772.41, 1608.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9383) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 260, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (313.33, 173.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698526 sequences (100000219 bp) in 3.208 CPU sec, 3.466 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 288482, 12, 105) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 250, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (266.03, 146.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (270.96, 134.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (344, 490, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3158) [M::mem_pestat] mean and std.dev: (933.83, 963.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 296, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (275.15, 118.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700228 reads in 199.152 CPU sec, 6.368 real sec [M::process] read 695938 sequences (100000213 bp) in 2.162 CPU sec, 2.204 real sec... [M::mem_process_seqs] Processed 699178 reads in 134.702 CPU sec, 4.269 real sec sorting block 28, #reads = 1083927 mem = 536879696 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 287689, 32, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 311, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (341.12, 206.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 253, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (276.73, 139.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 1039, 1521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4261) [M::mem_pestat] mean and std.dev: (900.42, 682.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5631) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 330, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (326.13, 150.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1403) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699792 sequences (100000148 bp) in 3.798 CPU sec, 4.203 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 288275, 15, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 391, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2828) [M::mem_pestat] mean and std.dev: (775.04, 853.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (170, 263, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (284.88, 141.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (364, 606, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3334) [M::mem_pestat] mean and std.dev: (562.08, 344.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4324) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 327, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (327.50, 140.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698526 reads in 191.719 CPU sec, 6.151 real sec [M::mem_process_seqs] Processed 695938 reads in 129.269 CPU sec, 4.105 real sec sorting block 29, #reads = 1084832 mem = 536880927 peak = 799211520 [M::process] read 699592 sequences (100000204 bp) in 2.728 CPU sec, 2.958 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 288170, 27, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 239, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (237.33, 92.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (270.52, 136.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (340, 880, 1367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3421) [M::mem_pestat] mean and std.dev: (979.00, 760.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 285, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (286.41, 156.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 30, #reads = 1083840 mem = 536879269 peak = 799211520 [M::process] read 699070 sequences (100000212 bp) in 2.700 CPU sec, 2.894 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 288765, 21, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 289, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (312.17, 147.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (272.41, 137.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (789, 914, 2051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4575) [M::mem_pestat] mean and std.dev: (1043.32, 596.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 347, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1529) [M::mem_pestat] mean and std.dev: (336.28, 222.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699792 reads in 195.105 CPU sec, 6.249 real sec [M::mem_process_seqs] Processed 699592 reads in 130.983 CPU sec, 4.144 real sec [M::process] read 698916 sequences (100000221 bp) in 2.184 CPU sec, 2.240 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 288676, 31, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 431, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2067) [M::mem_pestat] mean and std.dev: (465.12, 390.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 248, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (272.25, 136.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (573, 1235, 2260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5634) [M::mem_pestat] mean and std.dev: (1378.81, 1097.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 247, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (353.20, 272.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 31, #reads = 1086236 mem = 536879559 peak = 799211520 [M::process] read 698506 sequences (100000292 bp) in 3.395 CPU sec, 3.905 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 288209, 19, 96) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 314, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267) [M::mem_pestat] mean and std.dev: (413.84, 311.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (273.31, 137.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (705, 2285, 2285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5445) [M::mem_pestat] mean and std.dev: (1371.12, 1114.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7025) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 299, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215) [M::mem_pestat] mean and std.dev: (312.45, 185.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699070 reads in 194.992 CPU sec, 6.248 real sec [M::mem_process_seqs] Processed 698916 reads in 131.462 CPU sec, 4.169 real sec [M::process] read 694732 sequences (100000049 bp) in 2.803 CPU sec, 2.938 real sec... sorting block 32, #reads = 1086778 mem = 536887677 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (99, 288522, 20, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 250, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (279.30, 149.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (275.22, 139.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (551, 863, 2819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7355) [M::mem_pestat] mean and std.dev: (1557.45, 1335.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 245, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1535) [M::mem_pestat] mean and std.dev: (377.21, 261.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697706 sequences (100000064 bp) in 2.481 CPU sec, 2.717 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 286901, 30, 124) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 224, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1717) [M::mem_pestat] mean and std.dev: (377.57, 357.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (285.72, 141.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 702, 1541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4149) [M::mem_pestat] mean and std.dev: (795.07, 748.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 330, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (421.31, 295.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698506 reads in 192.005 CPU sec, 6.139 real sec [M::process] read 698152 sequences (100000255 bp) in 2.175 CPU sec, 2.221 real sec... [M::mem_process_seqs] Processed 694732 reads in 129.549 CPU sec, 4.112 real sec sorting block 33, #reads = 1085642 mem = 536877527 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 287964, 31, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 237, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 540) [M::mem_pestat] mean and std.dev: (223.00, 89.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (274.12, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (973, 1753, 2586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5812) [M::mem_pestat] mean and std.dev: (1851.83, 1376.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 384, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2018) [M::mem_pestat] mean and std.dev: (480.53, 455.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698030 sequences (100000090 bp) in 3.570 CPU sec, 3.956 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 287976, 30, 104) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 256, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (306.91, 237.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (273.58, 137.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (291, 399, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3090) [M::mem_pestat] mean and std.dev: (609.89, 613.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 337, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318) [M::mem_pestat] mean and std.dev: (361.26, 234.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697706 reads in 192.025 CPU sec, 6.153 real sec sorting block 34, #reads = 1084994 mem = 536878493 peak = 799211520 [M::mem_process_seqs] Processed 698152 reads in 129.178 CPU sec, 4.097 real sec [M::process] read 698184 sequences (100000086 bp) in 2.309 CPU sec, 2.400 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 287668, 32, 102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 330, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2989) [M::mem_pestat] mean and std.dev: (503.83, 498.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (273.08, 136.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (459, 875, 5831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16575) [M::mem_pestat] mean and std.dev: (2453.88, 2560.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 307, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (332.96, 178.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 35, #reads = 1086033 mem = 536880340 peak = 799211520 [M::process] read 698174 sequences (100000039 bp) in 3.102 CPU sec, 3.332 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 288003, 17, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 251, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (252.27, 121.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (270.95, 135.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (457, 723, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2989) [M::mem_pestat] mean and std.dev: (695.07, 459.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3833) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 256, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (327.72, 251.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698030 reads in 193.328 CPU sec, 6.230 real sec [M::mem_process_seqs] Processed 698184 reads in 130.517 CPU sec, 4.156 real sec [M::process] read 698474 sequences (100000040 bp) in 2.500 CPU sec, 2.630 real sec... sorting block 36, #reads = 1084938 mem = 536878493 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 287912, 31, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 301, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (373.94, 279.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (270.64, 135.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 518, 1430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3676) [M::mem_pestat] mean and std.dev: (879.26, 800.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 305, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1287) [M::mem_pestat] mean and std.dev: (389.16, 277.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698464 sequences (100000184 bp) in 2.904 CPU sec, 3.107 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 288057, 19, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 302, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (297.32, 158.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (269.53, 134.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (302, 1245, 1603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4205) [M::mem_pestat] mean and std.dev: (888.29, 701.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 249, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (231.42, 90.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698174 reads in 191.022 CPU sec, 6.123 real sec [M::process] read 698254 sequences (100000294 bp) in 1.821 CPU sec, 1.840 real sec... [M::mem_process_seqs] Processed 698474 reads in 128.685 CPU sec, 4.093 real sec sorting block 37, #reads = 1085200 mem = 536878918 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 287889, 22, 114) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 247, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435) [M::mem_pestat] mean and std.dev: (288.28, 251.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (269.99, 134.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (135, 877, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3093) [M::mem_pestat] mean and std.dev: (662.91, 494.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4079) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 354, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370) [M::mem_pestat] mean and std.dev: (378.12, 219.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698388 sequences (100000094 bp) in 3.221 CPU sec, 3.606 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (118, 288148, 10, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 329, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1482) [M::mem_pestat] mean and std.dev: (343.32, 206.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (271.57, 136.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1181, 1654, 2198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4232) [M::mem_pestat] mean and std.dev: (1515.00, 673.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 308, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (324.33, 161.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698464 reads in 194.801 CPU sec, 6.228 real sec sorting block 38, #reads = 1086204 mem = 536878856 peak = 799211520 [M::mem_process_seqs] Processed 698254 reads in 129.487 CPU sec, 4.085 real sec [M::process] read 698778 sequences (100000070 bp) in 2.660 CPU sec, 2.718 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 288013, 20, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 298, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250) [M::mem_pestat] mean and std.dev: (329.33, 202.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (271.43, 136.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (348, 591, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2853) [M::mem_pestat] mean and std.dev: (698.00, 521.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3688) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 265, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (303.00, 168.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 39, #reads = 1085391 mem = 536877504 peak = 799211520 [M::process] read 698668 sequences (100000248 bp) in 3.066 CPU sec, 3.418 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 288746, 36, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 231, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (264.06, 145.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (269.92, 136.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 753, 1763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4639) [M::mem_pestat] mean and std.dev: (998.43, 877.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 245, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442) [M::mem_pestat] mean and std.dev: (374.51, 287.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698388 reads in 194.268 CPU sec, 6.210 real sec [M::process] read 697052 sequences (100000250 bp) in 2.180 CPU sec, 2.214 real sec... [M::mem_process_seqs] Processed 698778 reads in 131.269 CPU sec, 4.169 real sec sorting block 40, #reads = 1084700 mem = 536879728 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 288037, 10, 105) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 284, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373) [M::mem_pestat] mean and std.dev: (363.89, 290.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (271.83, 134.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (408, 789, 2077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5415) [M::mem_pestat] mean and std.dev: (1001.22, 639.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 375, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (339.04, 153.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697338 sequences (100000263 bp) in 3.229 CPU sec, 3.578 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 286984, 17, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 361, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (346.63, 166.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 255, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (276.44, 136.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (460, 1046, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2218) [M::mem_pestat] mean and std.dev: (893.47, 448.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 240, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (252.39, 103.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698668 reads in 191.976 CPU sec, 6.171 real sec [M::process] read 698388 sequences (100000300 bp) in 2.167 CPU sec, 2.208 real sec... [M::mem_process_seqs] Processed 697052 reads in 129.595 CPU sec, 4.135 real sec sorting block 41, #reads = 1085081 mem = 536878516 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 287248, 14, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 269, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (306.18, 178.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (275.82, 136.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (646, 1607, 1927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4489) [M::mem_pestat] mean and std.dev: (1237.69, 761.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 282, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (329.90, 200.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696742 sequences (100000267 bp) in 2.970 CPU sec, 3.275 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 287673, 16, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 201, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (258.95, 151.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (271.94, 134.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (339, 521, 1256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3090) [M::mem_pestat] mean and std.dev: (640.31, 523.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4007) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 317, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449) [M::mem_pestat] mean and std.dev: (376.39, 262.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697338 reads in 185.644 CPU sec, 5.962 real sec [M::process] read 696952 sequences (100000121 bp) in 2.041 CPU sec, 2.098 real sec... [M::mem_process_seqs] Processed 698388 reads in 127.886 CPU sec, 4.059 real sec sorting block 42, #reads = 1085014 mem = 536889636 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 287402, 23, 109) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 235, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (261.17, 98.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 259, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (280.47, 139.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 705, 1153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3071) [M::mem_pestat] mean and std.dev: (764.74, 689.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4030) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 271, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1380) [M::mem_pestat] mean and std.dev: (373.26, 300.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 43, #reads = 1085404 mem = 536877372 peak = 799211520 [M::process] read 699756 sequences (100000231 bp) in 3.311 CPU sec, 3.566 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 287494, 31, 110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 244, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (214.50, 72.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 258, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (280.70, 140.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (437, 778, 1558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3800) [M::mem_pestat] mean and std.dev: (946.25, 645.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4921) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 247, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1954) [M::mem_pestat] mean and std.dev: (347.06, 346.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696742 reads in 187.210 CPU sec, 6.064 real sec [M::mem_process_seqs] Processed 696952 reads in 128.393 CPU sec, 4.149 real sec [M::process] read 700324 sequences (100000289 bp) in 2.340 CPU sec, 2.535 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (94, 288339, 33, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 218, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (251.55, 135.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (271.94, 137.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (349, 814, 1530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3892) [M::mem_pestat] mean and std.dev: (1018.61, 762.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 262, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (246.68, 105.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 44, #reads = 1084109 mem = 536882711 peak = 799211520 [M::process] read 700094 sequences (100000051 bp) in 3.030 CPU sec, 3.314 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 288938, 25, 102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 271, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (271.18, 157.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (270.51, 136.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (521, 819, 1408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3182) [M::mem_pestat] mean and std.dev: (941.20, 661.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4069) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 398, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429) [M::mem_pestat] mean and std.dev: (425.27, 277.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699756 reads in 188.890 CPU sec, 6.055 real sec [M::process] read 699652 sequences (100000136 bp) in 1.912 CPU sec, 1.981 real sec... [M::mem_process_seqs] Processed 700324 reads in 127.742 CPU sec, 4.054 real sec sorting block 45, #reads = 1086224 mem = 536881688 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 288267, 29, 98) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 256, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (295.65, 164.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (270.35, 136.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 1326, 2136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5210) [M::mem_pestat] mean and std.dev: (1434.19, 1345.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 258, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (266.50, 152.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698616 sequences (100000209 bp) in 3.470 CPU sec, 3.891 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 288610, 30, 101) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (252.98, 134.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (271.70, 137.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 1038, 2364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6756) [M::mem_pestat] mean and std.dev: (1311.10, 1015.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8952) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 329, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (304.63, 110.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700094 reads in 197.632 CPU sec, 6.363 real sec [M::process] read 696552 sequences (100000014 bp) in 2.365 CPU sec, 2.411 real sec... [M::mem_process_seqs] Processed 699652 reads in 134.022 CPU sec, 4.274 real sec sorting block 46, #reads = 1086449 mem = 536879169 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (94, 287471, 27, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (295.26, 146.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 251, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (275.72, 138.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 216, 2108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6302) [M::mem_pestat] mean and std.dev: (1108.44, 1528.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 413, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191) [M::mem_pestat] mean and std.dev: (434.81, 293.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699228 sequences (100000301 bp) in 3.139 CPU sec, 3.502 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 286846, 10, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 286, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (339.86, 169.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 258, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (280.36, 140.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (76, 611, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014) [M::mem_pestat] mean and std.dev: (301.62, 275.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2660) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 238, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (254.70, 109.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698616 reads in 190.282 CPU sec, 6.106 real sec sorting block 47, #reads = 1086728 mem = 536879190 peak = 799211520 [M::mem_process_seqs] Processed 696552 reads in 128.525 CPU sec, 4.080 real sec [M::process] read 697436 sequences (100000132 bp) in 2.339 CPU sec, 2.428 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 288555, 23, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 227, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (235.96, 94.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (272.38, 137.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 852, 1900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5572) [M::mem_pestat] mean and std.dev: (1006.81, 1200.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7408) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 278, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (289.01, 135.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 48, #reads = 1084772 mem = 536876205 peak = 799211520 [M::process] read 697960 sequences (100000265 bp) in 2.966 CPU sec, 3.215 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 287937, 29, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 234, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (263.70, 155.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (278.24, 140.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (534, 1191, 1684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3984) [M::mem_pestat] mean and std.dev: (1026.07, 565.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5134) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 325, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (349.03, 213.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699228 reads in 197.823 CPU sec, 6.369 real sec [M::mem_process_seqs] Processed 697436 reads in 132.226 CPU sec, 4.187 real sec [M::process] read 697748 sequences (100000147 bp) in 2.518 CPU sec, 2.609 real sec... sorting block 49, #reads = 1085167 mem = 536884680 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 287992, 26, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 279, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (273.00, 149.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 252, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (275.55, 138.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 686, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1963) [M::mem_pestat] mean and std.dev: (516.24, 407.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 371, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1655) [M::mem_pestat] mean and std.dev: (443.46, 341.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2132) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698396 sequences (100000066 bp) in 2.759 CPU sec, 3.028 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (98, 287779, 25, 94) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 292, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (303.33, 184.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (273.54, 137.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (83, 431, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2993) [M::mem_pestat] mean and std.dev: (544.04, 618.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 333, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (376.61, 218.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697960 reads in 192.482 CPU sec, 6.155 real sec [M::process] read 698832 sequences (100000135 bp) in 2.086 CPU sec, 2.093 real sec... [M::mem_process_seqs] Processed 697748 reads in 129.740 CPU sec, 4.121 real sec sorting block 50, #reads = 1085668 mem = 536879859 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 287959, 17, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 257, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (285.67, 152.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (270.57, 135.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 986, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2249) [M::mem_pestat] mean and std.dev: (853.35, 379.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 294, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (311.73, 169.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698564 sequences (100000177 bp) in 3.179 CPU sec, 3.459 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (94, 288707, 22, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 305, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1751) [M::mem_pestat] mean and std.dev: (415.34, 380.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (269.81, 135.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (150, 753, 2101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6003) [M::mem_pestat] mean and std.dev: (1111.24, 1279.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 280, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125) [M::mem_pestat] mean and std.dev: (333.79, 260.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698710 sequences (100000048 bp) in 2.020 CPU sec, 2.221 real sec... [M::mem_process_seqs] Processed 698396 reads in 196.122 CPU sec, 6.288 real sec sorting block 51, #reads = 1085413 mem = 536879279 peak = 799211520 [M::mem_process_seqs] Processed 698832 reads in 135.638 CPU sec, 4.368 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 288283, 22, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 257, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (300.96, 211.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (270.19, 135.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 608, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3027) [M::mem_pestat] mean and std.dev: (660.95, 712.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 309, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (335.72, 229.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 52, #reads = 1084650 mem = 536876789 peak = 799211520 [M::process] read 698766 sequences (100000145 bp) in 3.013 CPU sec, 3.280 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 288107, 29, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 226, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200) [M::mem_pestat] mean and std.dev: (300.77, 224.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (271.33, 136.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 566, 1914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4948) [M::mem_pestat] mean and std.dev: (781.85, 735.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 262, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (269.52, 164.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698564 reads in 195.391 CPU sec, 6.254 real sec [M::process] read 704442 sequences (100000016 bp) in 2.215 CPU sec, 2.245 real sec... [M::mem_process_seqs] Processed 698710 reads in 128.344 CPU sec, 4.063 real sec sorting block 53, #reads = 1084417 mem = 536878913 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 288065, 28, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 236, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1804) [M::mem_pestat] mean and std.dev: (367.70, 374.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (270.13, 136.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (346, 1071, 1733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4507) [M::mem_pestat] mean and std.dev: (1026.69, 744.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 263, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (311.43, 196.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701892 sequences (100000065 bp) in 3.234 CPU sec, 3.375 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 290417, 20, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 282, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (328.86, 187.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 229, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (257.81, 133.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 562, 1317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3571) [M::mem_pestat] mean and std.dev: (614.65, 681.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 256, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (281.55, 156.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698766 reads in 202.014 CPU sec, 6.446 real sec [M::process] read 703092 sequences (100000035 bp) in 2.182 CPU sec, 2.191 real sec... [M::mem_process_seqs] Processed 704442 reads in 139.307 CPU sec, 4.408 real sec sorting block 54, #reads = 1085833 mem = 536886481 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 289493, 19, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 270, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186) [M::mem_pestat] mean and std.dev: (304.23, 180.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (270.03, 141.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (417, 666, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1992) [M::mem_pestat] mean and std.dev: (582.76, 263.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 327, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524) [M::mem_pestat] mean and std.dev: (365.79, 272.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703940 sequences (100000026 bp) in 3.411 CPU sec, 3.730 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 289943, 41, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 235, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (301.42, 210.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 236, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (265.74, 139.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 195, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3558) [M::mem_pestat] mean and std.dev: (727.25, 858.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 301, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (296.82, 156.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701892 reads in 204.619 CPU sec, 6.541 real sec [M::process] read 704340 sequences (100000210 bp) in 2.258 CPU sec, 2.281 real sec... [M::mem_process_seqs] Processed 703092 reads in 138.370 CPU sec, 4.389 real sec sorting block 55, #reads = 1084551 mem = 536881436 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 290467, 31, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 263, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198) [M::mem_pestat] mean and std.dev: (315.09, 191.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 232, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (261.81, 136.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (271, 417, 1746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4696) [M::mem_pestat] mean and std.dev: (792.00, 856.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6171) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 262, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (249.42, 112.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 56, #reads = 1084966 mem = 536880888 peak = 799211520 [M::process] read 700410 sequences (100000236 bp) in 3.298 CPU sec, 3.520 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 290314, 13, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 238, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166) [M::mem_pestat] mean and std.dev: (285.17, 186.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (259.11, 133.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (348, 827, 1934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5106) [M::mem_pestat] mean and std.dev: (1400.31, 1529.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 290, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (300.50, 132.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703502 sequences (100000112 bp) in 2.150 CPU sec, 2.147 real sec... [M::mem_process_seqs] Processed 703940 reads in 201.007 CPU sec, 6.401 real sec [M::mem_process_seqs] Processed 704340 reads in 135.694 CPU sec, 4.292 real sec sorting block 57, #reads = 1087217 mem = 536881625 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 289431, 20, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 209, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (292.19, 260.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 246, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (274.81, 142.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (417, 1141, 3195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8751) [M::mem_pestat] mean and std.dev: (1448.11, 1477.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 260, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (318.79, 184.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704312 sequences (100000226 bp) in 3.464 CPU sec, 3.785 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 290380, 30, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 273, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197) [M::mem_pestat] mean and std.dev: (315.71, 245.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 234, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (263.46, 136.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (525, 916, 2308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5874) [M::mem_pestat] mean and std.dev: (1220.38, 1353.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 256, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (272.79, 158.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700410 reads in 203.313 CPU sec, 6.473 real sec [M::process] read 702430 sequences (100000094 bp) in 2.107 CPU sec, 2.108 real sec... [M::mem_process_seqs] Processed 703502 reads in 139.172 CPU sec, 4.420 real sec sorting block 58, #reads = 1083582 mem = 536881353 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 290502, 18, 109) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 298, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253) [M::mem_pestat] mean and std.dev: (352.29, 240.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (260.82, 135.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (435, 689, 1066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328) [M::mem_pestat] mean and std.dev: (661.47, 456.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 347, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (351.62, 165.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698846 sequences (100000049 bp) in 2.997 CPU sec, 3.284 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 289659, 19, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349) [M::mem_pestat] mean and std.dev: (333.88, 257.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (266.56, 138.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (114, 397, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1563) [M::mem_pestat] mean and std.dev: (344.65, 262.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2046) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 287, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562) [M::mem_pestat] mean and std.dev: (365.43, 239.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700138 sequences (100000197 bp) in 2.181 CPU sec, 2.183 real sec... [M::mem_process_seqs] Processed 704312 reads in 207.066 CPU sec, 6.602 real sec sorting block 59, #reads = 1085493 mem = 536881629 peak = 799211520 [M::mem_process_seqs] Processed 702430 reads in 138.363 CPU sec, 4.377 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 288195, 26, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 286, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (254.36, 128.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 250, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (277.51, 142.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 991) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 576, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1964) [M::mem_pestat] mean and std.dev: (596.77, 393.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2555) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 309, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (321.92, 168.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698966 sequences (100000060 bp) in 2.969 CPU sec, 3.175 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 288913, 20, 93) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 296, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144) [M::mem_pestat] mean and std.dev: (288.31, 177.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (272.54, 140.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (154, 610, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2866) [M::mem_pestat] mean and std.dev: (704.37, 718.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 310, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (328.83, 209.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699234 sequences (100000000 bp) in 2.132 CPU sec, 2.145 real sec... [M::mem_process_seqs] Processed 698846 reads in 206.203 CPU sec, 6.599 real sec sorting block 60, #reads = 1084175 mem = 536879149 peak = 799211520 [M::mem_process_seqs] Processed 700138 reads in 139.363 CPU sec, 4.465 real sec sorting block 61, #reads = 1082772 mem = 536879751 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 288486, 13, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (117, 268, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278) [M::mem_pestat] mean and std.dev: (360.01, 291.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 248, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (275.20, 141.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (113, 785, 2331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6767) [M::mem_pestat] mean and std.dev: (915.33, 919.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8985) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 288, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143) [M::mem_pestat] mean and std.dev: (319.38, 222.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699162 sequences (100000279 bp) in 3.488 CPU sec, 3.799 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 288413, 28, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 213, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (249.53, 155.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (270.69, 137.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (507, 1525, 2131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5379) [M::mem_pestat] mean and std.dev: (1541.29, 1154.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 357, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (346.72, 210.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696684 sequences (100000146 bp) in 2.061 CPU sec, 2.071 real sec... [M::mem_process_seqs] Processed 698966 reads in 208.866 CPU sec, 6.673 real sec [M::mem_process_seqs] Processed 699234 reads in 144.181 CPU sec, 4.603 real sec sorting block 62, #reads = 1082358 mem = 536878985 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 288101, 16, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 320, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1387) [M::mem_pestat] mean and std.dev: (383.12, 297.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (271.31, 137.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (442, 521, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (186, 826) [M::mem_pestat] mean and std.dev: (442.92, 112.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 263, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (282.27, 159.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699564 sequences (100000183 bp) in 2.998 CPU sec, 3.256 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 288184, 35, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 223, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (251.17, 143.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 255, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (281.83, 143.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 495, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2105) [M::mem_pestat] mean and std.dev: (420.21, 295.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 296, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (350.75, 243.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698738 sequences (100000185 bp) in 1.808 CPU sec, 1.809 real sec... [M::mem_process_seqs] Processed 699162 reads in 208.130 CPU sec, 6.661 real sec [M::mem_process_seqs] Processed 696684 reads in 141.120 CPU sec, 4.493 real sec sorting block 63, #reads = 1080140 mem = 536880681 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 288526, 21, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 303, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (416.20, 310.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (269.68, 136.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (400, 1177, 1584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3952) [M::mem_pestat] mean and std.dev: (1241.50, 1062.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5491) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 308, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (299.03, 142.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698656 sequences (100000060 bp) in 3.657 CPU sec, 4.082 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 287798, 29, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 277, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (350.17, 248.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (272.98, 138.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (576, 1127, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2682) [M::mem_pestat] mean and std.dev: (897.81, 514.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 248, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032) [M::mem_pestat] mean and std.dev: (337.63, 238.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699340 sequences (100000188 bp) in 2.178 CPU sec, 2.199 real sec... [M::mem_process_seqs] Processed 699564 reads in 213.048 CPU sec, 6.813 real sec sorting block 64, #reads = 1080536 mem = 536881109 peak = 799211520 [M::mem_process_seqs] Processed 698738 reads in 141.430 CPU sec, 4.514 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 288010, 17, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 347, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136) [M::mem_pestat] mean and std.dev: (302.15, 166.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (272.98, 138.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (449, 449, 2239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5819) [M::mem_pestat] mean and std.dev: (1334.94, 1076.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 381, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1622) [M::mem_pestat] mean and std.dev: (437.52, 349.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 65, #reads = 1082463 mem = 536882622 peak = 799211520 [M::process] read 699406 sequences (100000060 bp) in 3.301 CPU sec, 3.629 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 288584, 24, 95) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 369, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1549) [M::mem_pestat] mean and std.dev: (415.33, 308.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (271.30, 138.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (372, 949, 1513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3795) [M::mem_pestat] mean and std.dev: (874.77, 688.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 273, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (267.90, 135.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698656 reads in 209.127 CPU sec, 6.715 real sec [M::process] read 708212 sequences (100000082 bp) in 2.297 CPU sec, 2.319 real sec... [M::mem_process_seqs] Processed 699340 reads in 143.128 CPU sec, 4.588 real sec sorting block 66, #reads = 1081077 mem = 536881499 peak = 799211520 [M::process] read 705356 sequences (100000213 bp) in 3.420 CPU sec, 3.699 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 291376, 26, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 282, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (305.10, 212.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 704984 sequences (100000266 bp) in 2.233 CPU sec, 2.235 real sec... [M::mem_pestat] (25, 50, 75) percentile: (149, 222, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (250.11, 128.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 467, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2039) [M::mem_pestat] mean and std.dev: (500.58, 464.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 230, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (339.96, 245.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1322) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 708212 reads in 136.324 CPU sec, 4.324 real sec sorting block 67, #reads = 1079748 mem = 536880512 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 288382, 21, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 266, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (259.98, 113.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.02, 140.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (208, 755, 1929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5371) [M::mem_pestat] mean and std.dev: (983.89, 1016.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 269, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (279.68, 144.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 290298, 10, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115) [M::mem_pestat] mean and std.dev: (322.44, 219.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 231, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (258.18, 132.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (385, 708, 2636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7138) [M::mem_pestat] mean and std.dev: (1331.10, 1368.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 207, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (319.15, 244.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704752 sequences (100000208 bp) in 2.724 CPU sec, 2.726 real sec... [M::mem_process_seqs] Processed 699406 reads in 363.146 CPU sec, 11.575 real sec [M::mem_process_seqs] Processed 705356 reads in 185.358 CPU sec, 5.992 real sec sorting block 68, #reads = 1080076 mem = 536881703 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 290742, 27, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 288, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1514) [M::mem_pestat] mean and std.dev: (387.71, 279.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 231, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (258.50, 132.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (198, 915, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3582) [M::mem_pestat] mean and std.dev: (882.19, 743.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 294, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (276.15, 126.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702782 sequences (100000253 bp) in 3.190 CPU sec, 3.416 real sec... [M::process] read 703754 sequences (100000112 bp) in 1.999 CPU sec, 2.001 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 290520, 27, 95) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 227, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (269.75, 152.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 230, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (257.66, 131.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 470, 3059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8581) [M::mem_pestat] mean and std.dev: (1277.41, 1200.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 313, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1380) [M::mem_pestat] mean and std.dev: (423.01, 311.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704984 reads in 224.420 CPU sec, 7.172 real sec [M::mem_process_seqs] Processed 704752 reads in 152.068 CPU sec, 4.853 real sec sorting block 69, #reads = 1079313 mem = 536884435 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 289531, 27, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 236, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (280.86, 134.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 237, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (263.15, 134.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 843, 1712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5056) [M::mem_pestat] mean and std.dev: (864.96, 883.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 307, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007) [M::mem_pestat] mean and std.dev: (313.49, 158.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704584 sequences (100000097 bp) in 3.431 CPU sec, 3.753 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 290262, 24, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 248, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151) [M::mem_pestat] mean and std.dev: (281.67, 163.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 233, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (259.41, 131.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (114, 361, 2476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7200) [M::mem_pestat] mean and std.dev: (940.71, 1107.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 285, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (262.58, 111.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703812 sequences (100000008 bp) in 2.281 CPU sec, 2.290 real sec... [M::mem_process_seqs] Processed 702782 reads in 218.425 CPU sec, 6.970 real sec sorting block 70, #reads = 1081286 mem = 536878516 peak = 799211520 [M::mem_process_seqs] Processed 703754 reads in 147.155 CPU sec, 4.690 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 290404, 22, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 360, 1257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3389) [M::mem_pestat] mean and std.dev: (746.34, 795.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (258.46, 132.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (419, 635, 2539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6779) [M::mem_pestat] mean and std.dev: (1142.52, 1002.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 229, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (261.14, 159.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 71, #reads = 1083396 mem = 536883061 peak = 799211520 [M::process] read 700128 sequences (100000162 bp) in 2.907 CPU sec, 3.225 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 289824, 19, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 229, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1683) [M::mem_pestat] mean and std.dev: (345.92, 280.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 234, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (261.00, 133.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (313, 524, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3652) [M::mem_pestat] mean and std.dev: (641.06, 511.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 284, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (331.11, 196.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1574) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701184 sequences (100000200 bp) in 2.250 CPU sec, 2.260 real sec... [M::mem_process_seqs] Processed 704584 reads in 227.350 CPU sec, 7.283 real sec [M::mem_process_seqs] Processed 703812 reads in 153.093 CPU sec, 4.878 real sec sorting block 72, #reads = 1083847 mem = 536881480 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 289193, 25, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 228, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (245.77, 139.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 246, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (272.87, 139.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (373, 613, 2124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5626) [M::mem_pestat] mean and std.dev: (1048.24, 882.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 316, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (331.36, 149.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699944 sequences (100000148 bp) in 3.575 CPU sec, 3.878 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 289212, 19, 101) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 277, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (266.05, 139.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (269.41, 138.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (476, 846, 1718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4202) [M::mem_pestat] mean and std.dev: (1102.79, 1015.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5444) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 248, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (288.99, 215.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699808 sequences (100000147 bp) in 1.910 CPU sec, 1.921 real sec... [M::mem_process_seqs] Processed 700128 reads in 213.527 CPU sec, 6.810 real sec [M::mem_process_seqs] Processed 701184 reads in 145.898 CPU sec, 4.663 real sec sorting block 73, #reads = 1082728 mem = 536882078 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 288521, 22, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 275, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315) [M::mem_pestat] mean and std.dev: (347.15, 232.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (272.09, 139.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 664, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2010) [M::mem_pestat] mean and std.dev: (497.48, 377.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2618) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 288, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (286.63, 164.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699594 sequences (100000046 bp) in 2.702 CPU sec, 3.012 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 288808, 16, 139) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (294.25, 166.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.04, 138.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 1138, 2245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6399) [M::mem_pestat] mean and std.dev: (1490.44, 1501.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 263, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301) [M::mem_pestat] mean and std.dev: (313.52, 216.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1674) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696848 sequences (100000033 bp) in 1.912 CPU sec, 1.946 real sec... [M::mem_process_seqs] Processed 699944 reads in 212.459 CPU sec, 6.834 real sec sorting block 74, #reads = 1081545 mem = 536879477 peak = 799211520 [M::mem_process_seqs] Processed 699808 reads in 143.130 CPU sec, 4.594 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 288667, 20, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 290, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292) [M::mem_pestat] mean and std.dev: (323.58, 253.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.43, 138.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 531, 1884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5212) [M::mem_pestat] mean and std.dev: (1315.45, 1535.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 315, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272) [M::mem_pestat] mean and std.dev: (316.00, 183.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1633) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699752 sequences (100000024 bp) in 3.116 CPU sec, 3.383 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 287614, 20, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 219, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (261.01, 171.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 254, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (281.06, 143.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 631, 2087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5811) [M::mem_pestat] mean and std.dev: (1114.32, 1374.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 310, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1386) [M::mem_pestat] mean and std.dev: (376.88, 245.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699364 sequences (100000190 bp) in 2.266 CPU sec, 2.340 real sec... [M::mem_process_seqs] Processed 699594 reads in 211.626 CPU sec, 6.769 real sec sorting block 75, #reads = 1081547 mem = 536882413 peak = 799211520 [M::mem_process_seqs] Processed 696848 reads in 139.266 CPU sec, 4.440 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 288646, 14, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 273, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2056) [M::mem_pestat] mean and std.dev: (357.76, 333.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (269.85, 137.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 254, 905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2329) [M::mem_pestat] mean and std.dev: (562.77, 650.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3166) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 352, 1143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013) [M::mem_pestat] mean and std.dev: (586.01, 633.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 76, #reads = 1082960 mem = 536877655 peak = 799211520 [M::process] read 699806 sequences (100000236 bp) in 3.035 CPU sec, 3.342 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 288529, 23, 101) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 246, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (261.30, 145.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (272.27, 139.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (804, 1073, 3065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7587) [M::mem_pestat] mean and std.dev: (2236.91, 2172.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 279, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424) [M::mem_pestat] mean and std.dev: (373.58, 265.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700404 sequences (100000221 bp) in 2.099 CPU sec, 2.109 real sec... [M::mem_process_seqs] Processed 699752 reads in 208.448 CPU sec, 6.695 real sec [M::mem_process_seqs] Processed 699364 reads in 142.235 CPU sec, 4.534 real sec sorting block 77, #reads = 1081796 mem = 536877471 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 288381, 19, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 282, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (264.84, 131.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (270.36, 139.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 663, 1744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4444) [M::mem_pestat] mean and std.dev: (1077.11, 996.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5794) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 310, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (288.84, 159.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703776 sequences (100000060 bp) in 3.616 CPU sec, 3.880 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 288154, 24, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 339, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1878) [M::mem_pestat] mean and std.dev: (449.33, 391.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (269.07, 138.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 1042, 1704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4564) [M::mem_pestat] mean and std.dev: (932.00, 733.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5994) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 274, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (303.37, 172.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703890 sequences (100000278 bp) in 2.149 CPU sec, 2.150 real sec... [M::mem_process_seqs] Processed 699806 reads in 220.428 CPU sec, 7.060 real sec [M::mem_process_seqs] Processed 700404 reads in 147.900 CPU sec, 4.726 real sec sorting block 78, #reads = 1081939 mem = 536879268 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 289088, 14, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 258, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434) [M::mem_pestat] mean and std.dev: (371.17, 303.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (260.80, 134.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (248, 1132, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5009) [M::mem_pestat] mean and std.dev: (1229.07, 1067.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 250, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (253.12, 112.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703142 sequences (100000082 bp) in 2.886 CPU sec, 3.178 real sec... [M::process] read 702850 sequences (100000301 bp) in 2.175 CPU sec, 2.246 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 290422, 17, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 285, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1557) [M::mem_pestat] mean and std.dev: (398.14, 311.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 234, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (262.41, 134.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (219, 501, 1650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4512) [M::mem_pestat] mean and std.dev: (762.47, 651.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 274, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (310.91, 191.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703776 reads in 233.646 CPU sec, 7.528 real sec sorting block 79, #reads = 1077566 mem = 536877810 peak = 799211520 [M::mem_process_seqs] Processed 703890 reads in 146.393 CPU sec, 4.713 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 289622, 39, 107) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 278, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1845) [M::mem_pestat] mean and std.dev: (412.45, 379.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 236, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (263.18, 134.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (847, 972, 4639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12223) [M::mem_pestat] mean and std.dev: (2835.18, 2950.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16015) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 347, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1423) [M::mem_pestat] mean and std.dev: (410.56, 320.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 80, #reads = 1080137 mem = 536877982 peak = 799211520 [M::process] read 699688 sequences (100000179 bp) in 3.175 CPU sec, 3.736 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 289736, 10, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 234, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279) [M::mem_pestat] mean and std.dev: (318.01, 236.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 237, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (265.30, 136.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 956, 1434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3800) [M::mem_pestat] mean and std.dev: (766.78, 560.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 265, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (286.73, 154.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701850 sequences (100000270 bp) in 2.137 CPU sec, 2.152 real sec... [M::mem_process_seqs] Processed 703142 reads in 209.829 CPU sec, 6.743 real sec [M::mem_process_seqs] Processed 702850 reads in 137.374 CPU sec, 4.376 real sec sorting block 81, #reads = 1085951 mem = 536879090 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 288989, 29, 110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 270, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1369) [M::mem_pestat] mean and std.dev: (343.65, 261.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (273.77, 139.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (445, 783, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3847) [M::mem_pestat] mean and std.dev: (946.83, 739.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4981) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 243, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (261.05, 155.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703956 sequences (100000221 bp) in 3.249 CPU sec, 3.596 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 289722, 18, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 403, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1631) [M::mem_pestat] mean and std.dev: (547.27, 444.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 240, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (266.80, 136.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 487, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1874) [M::mem_pestat] mean and std.dev: (390.65, 362.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 257, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (268.95, 167.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699688 reads in 209.109 CPU sec, 6.713 real sec [M::process] read 704168 sequences (100000258 bp) in 2.411 CPU sec, 2.521 real sec... [M::mem_process_seqs] Processed 701850 reads in 141.903 CPU sec, 4.518 real sec sorting block 82, #reads = 1086578 mem = 536879787 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 290077, 13, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 278, 1596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4440) [M::mem_pestat] mean and std.dev: (806.82, 1065.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 233, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (258.87, 131.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 385, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2275) [M::mem_pestat] mean and std.dev: (598.15, 495.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 275, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (275.46, 141.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702898 sequences (100000077 bp) in 3.429 CPU sec, 3.775 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 290052, 30, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 342, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184) [M::mem_pestat] mean and std.dev: (387.31, 242.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 232, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (258.49, 131.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (315, 445, 1825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4845) [M::mem_pestat] mean and std.dev: (1214.52, 1332.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 222, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (237.51, 114.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703956 reads in 215.445 CPU sec, 6.914 real sec [M::process] read 702304 sequences (100000281 bp) in 2.381 CPU sec, 2.463 real sec... sorting block 83, #reads = 1085300 mem = 536880093 peak = 799211520 [M::mem_process_seqs] Processed 704168 reads in 144.508 CPU sec, 4.603 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 290171, 36, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 189, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (219.40, 123.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (261.50, 132.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 635, 1433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4181) [M::mem_pestat] mean and std.dev: (755.88, 894.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5555) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 227, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (285.37, 177.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699552 sequences (100000266 bp) in 3.110 CPU sec, 3.464 real sec... sorting block 84, #reads = 1085808 mem = 536877194 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 289751, 21, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 273, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026) [M::mem_pestat] mean and std.dev: (279.66, 180.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (264.64, 135.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (344, 531, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2627) [M::mem_pestat] mean and std.dev: (573.25, 460.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 299, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1840) [M::mem_pestat] mean and std.dev: (324.65, 238.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702898 reads in 210.145 CPU sec, 6.784 real sec [M::mem_process_seqs] Processed 702304 reads in 141.156 CPU sec, 4.491 real sec [M::process] read 701524 sequences (100000228 bp) in 2.467 CPU sec, 2.528 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 288844, 35, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 257, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (243.46, 117.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (273.36, 138.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (500, 746, 1591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3773) [M::mem_pestat] mean and std.dev: (835.19, 578.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 294, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (303.62, 137.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 85, #reads = 1086822 mem = 536878003 peak = 799211520 [M::process] read 701716 sequences (100000176 bp) in 3.582 CPU sec, 4.045 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 289596, 15, 106) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 255, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (306.92, 193.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (265.77, 134.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (476, 744, 1751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4301) [M::mem_pestat] mean and std.dev: (971.77, 771.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 250, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (266.73, 131.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699552 reads in 207.336 CPU sec, 6.674 real sec [M::mem_process_seqs] Processed 701524 reads in 140.103 CPU sec, 4.462 real sec [M::process] read 700846 sequences (100000031 bp) in 2.747 CPU sec, 2.907 real sec... sorting block 86, #reads = 1084307 mem = 536877427 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 289118, 20, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (241.68, 108.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (265.19, 134.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (350, 464, 1913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5039) [M::mem_pestat] mean and std.dev: (772.12, 712.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 262, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (274.04, 116.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700362 sequences (100000245 bp) in 2.962 CPU sec, 3.507 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 289082, 26, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 180, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (207.71, 101.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (268.33, 136.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (387, 607, 3060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8406) [M::mem_pestat] mean and std.dev: (1478.92, 1326.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11079) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 272, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (323.86, 181.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701716 reads in 213.046 CPU sec, 6.883 real sec [M::process] read 700622 sequences (100000074 bp) in 2.408 CPU sec, 2.606 real sec... sorting block 87, #reads = 1086088 mem = 536878002 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 289159, 26, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 253, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (267.31, 147.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (270.23, 138.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (419, 521, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1775) [M::mem_pestat] mean and std.dev: (535.18, 413.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 355, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2410) [M::mem_pestat] mean and std.dev: (517.42, 494.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700732 sequences (100000140 bp) in 2.423 CPU sec, 2.442 real sec... [M::mem_process_seqs] Processed 700362 reads in 126.315 CPU sec, 4.024 real sec [M::mem_process_seqs] Processed 700846 reads in 319.551 CPU sec, 10.258 real sec sorting block 88, #reads = 1084297 mem = 536879642 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 288861, 19, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 345, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443) [M::mem_pestat] mean and std.dev: (413.82, 301.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (269.85, 138.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 600, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794) [M::mem_pestat] mean and std.dev: (516.59, 391.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 501, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2947) [M::mem_pestat] mean and std.dev: (626.39, 575.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700190 sequences (100000035 bp) in 3.266 CPU sec, 3.685 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 288655, 23, 107) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 272, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454) [M::mem_pestat] mean and std.dev: (376.43, 265.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (269.17, 138.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (748, 1159, 3397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8695) [M::mem_pestat] mean and std.dev: (1880.74, 1625.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 312, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (315.12, 129.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700622 reads in 246.516 CPU sec, 7.879 real sec [M::mem_process_seqs] Processed 700732 reads in 152.289 CPU sec, 4.842 real sec [M::process] read 701194 sequences (100000236 bp) in 2.384 CPU sec, 2.678 real sec... sorting block 89, #reads = 1082915 mem = 536883345 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (100, 288458, 27, 94) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 280, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (255.48, 115.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (271.04, 139.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 477, 1040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2510) [M::mem_pestat] mean and std.dev: (564.54, 455.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 369, 861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2037) [M::mem_pestat] mean and std.dev: (513.32, 432.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2625) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702062 sequences (100000036 bp) in 3.189 CPU sec, 3.490 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 289169, 24, 103) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 233, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (257.42, 170.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 238, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (265.44, 136.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (740, 1088, 5135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13925) [M::mem_pestat] mean and std.dev: (2710.42, 3124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 264, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (300.47, 161.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707886 sequences (100000109 bp) in 2.152 CPU sec, 2.161 real sec... [M::mem_process_seqs] Processed 700190 reads in 221.083 CPU sec, 7.065 real sec sorting block 90, #reads = 1081321 mem = 536879503 peak = 799211520 [M::mem_process_seqs] Processed 701194 reads in 148.309 CPU sec, 4.733 real sec sorting block 91, #reads = 1082068 mem = 536878545 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 288853, 18, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 243, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (273.89, 183.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (261.47, 133.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 677, 1028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1804) [M::mem_pestat] mean and std.dev: (717.00, 330.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 310, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417) [M::mem_pestat] mean and std.dev: (330.12, 232.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705800 sequences (100000127 bp) in 2.739 CPU sec, 3.006 real sec... [M::process] read 706964 sequences (100000289 bp) in 2.165 CPU sec, 2.174 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 290752, 32, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 326, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1342) [M::mem_pestat] mean and std.dev: (394.33, 270.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 224, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (254.10, 131.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (332, 859, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3845) [M::mem_pestat] mean and std.dev: (1066.53, 962.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5016) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 333, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (356.93, 241.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702062 reads in 250.757 CPU sec, 8.063 real sec [M::mem_process_seqs] Processed 707886 reads in 168.886 CPU sec, 5.417 real sec sorting block 92, #reads = 1074232 mem = 536876895 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 290618, 29, 104) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (242.98, 148.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 229, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (258.20, 132.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (429, 1004, 1946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4980) [M::mem_pestat] mean and std.dev: (1035.04, 899.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 431, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1379) [M::mem_pestat] mean and std.dev: (384.18, 234.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702724 sequences (100000205 bp) in 3.150 CPU sec, 3.701 real sec... [M::process] read 705352 sequences (100000162 bp) in 2.557 CPU sec, 2.571 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 291177, 33, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 242, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441) [M::mem_pestat] mean and std.dev: (286.17, 240.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 225, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (253.13, 129.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (804, 1788, 3277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8223) [M::mem_pestat] mean and std.dev: (1520.07, 981.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10696) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 303, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (293.61, 140.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705800 reads in 246.722 CPU sec, 7.915 real sec [M::mem_process_seqs] Processed 706964 reads in 161.575 CPU sec, 5.152 real sec sorting block 93, #reads = 1079620 mem = 536877381 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 290154, 14, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 335, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1777) [M::mem_pestat] mean and std.dev: (425.06, 347.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 240, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (267.79, 137.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (738, 964, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2424) [M::mem_pestat] mean and std.dev: (878.54, 468.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 279, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064) [M::mem_pestat] mean and std.dev: (334.65, 182.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706830 sequences (100000108 bp) in 3.222 CPU sec, 3.624 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 290069, 22, 105) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 313, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (296.32, 169.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 229, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (257.95, 132.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 901, 2297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6827) [M::mem_pestat] mean and std.dev: (1099.41, 1027.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 302, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (273.01, 136.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706096 sequences (100000188 bp) in 2.282 CPU sec, 2.451 real sec... [M::mem_process_seqs] Processed 702724 reads in 234.784 CPU sec, 7.582 real sec sorting block 94, #reads = 1079730 mem = 536879332 peak = 799211520 [M::mem_process_seqs] Processed 705352 reads in 154.493 CPU sec, 4.969 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 291077, 9, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 284, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309) [M::mem_pestat] mean and std.dev: (303.62, 196.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 226, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (254.09, 130.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 251, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (240.24, 100.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 95, #reads = 1081273 mem = 536882298 peak = 799211520 [M::process] read 704476 sequences (100000181 bp) in 2.994 CPU sec, 3.498 real sec... [M::process] read 701896 sequences (100000194 bp) in 2.221 CPU sec, 2.249 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 290913, 15, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 247, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2193) [M::mem_pestat] mean and std.dev: (420.67, 414.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 227, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (252.40, 127.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (339, 339, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764) [M::mem_pestat] mean and std.dev: (417.54, 304.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 340, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2340) [M::mem_pestat] mean and std.dev: (496.73, 377.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706830 reads in 231.626 CPU sec, 7.461 real sec [M::mem_process_seqs] Processed 706096 reads in 152.992 CPU sec, 4.883 real sec sorting block 96, #reads = 1084847 mem = 536881148 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 290256, 25, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 211, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1552) [M::mem_pestat] mean and std.dev: (310.91, 255.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (257.35, 130.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 672, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2370) [M::mem_pestat] mean and std.dev: (515.59, 405.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3090) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 305, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (301.23, 151.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702130 sequences (100000233 bp) in 3.308 CPU sec, 3.682 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 289659, 37, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 329, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2623) [M::mem_pestat] mean and std.dev: (496.12, 536.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 240, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (266.25, 134.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (449, 1046, 2889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7769) [M::mem_pestat] mean and std.dev: (1337.28, 1288.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 329, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (305.95, 127.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703018 sequences (100000176 bp) in 2.259 CPU sec, 2.301 real sec... [M::mem_process_seqs] Processed 704476 reads in 223.731 CPU sec, 7.194 real sec [M::mem_process_seqs] Processed 701896 reads in 148.609 CPU sec, 4.762 real sec sorting block 97, #reads = 1082736 mem = 536886059 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 289813, 27, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 277, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (253.10, 117.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (263.70, 133.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 885, 2368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6504) [M::mem_pestat] mean and std.dev: (1295.12, 1628.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 428, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1537) [M::mem_pestat] mean and std.dev: (448.20, 305.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701948 sequences (100000039 bp) in 3.467 CPU sec, 4.054 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 289804, 24, 87) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 225, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (245.76, 154.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (260.77, 131.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (347, 627, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2645) [M::mem_pestat] mean and std.dev: (810.70, 594.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 332, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1594) [M::mem_pestat] mean and std.dev: (434.23, 352.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701912 sequences (100000038 bp) in 2.093 CPU sec, 2.100 real sec... [M::mem_process_seqs] Processed 702130 reads in 221.645 CPU sec, 7.104 real sec sorting block 98, #reads = 1081654 mem = 536880631 peak = 799211520 [M::mem_process_seqs] Processed 703018 reads in 146.655 CPU sec, 4.678 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (100, 289525, 19, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 314, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (308.61, 173.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (265.49, 134.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (367, 561, 2841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7789) [M::mem_pestat] mean and std.dev: (1311.22, 1384.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 273, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (260.18, 127.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 99, #reads = 1085092 mem = 536880230 peak = 799211520 [M::process] read 700640 sequences (100000110 bp) in 3.179 CPU sec, 3.766 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 289319, 22, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 259, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (269.48, 140.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (264.01, 133.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (320, 481, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1886) [M::mem_pestat] mean and std.dev: (509.89, 299.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2408) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 383, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (423.23, 334.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1937) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700670 sequences (100000154 bp) in 2.211 CPU sec, 2.228 real sec... [M::mem_process_seqs] Processed 701948 reads in 220.736 CPU sec, 7.141 real sec [M::mem_process_seqs] Processed 701912 reads in 148.399 CPU sec, 4.803 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 289141, 18, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 270, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166) [M::mem_pestat] mean and std.dev: (307.07, 207.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (267.46, 135.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (493, 2046, 4746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13252) [M::mem_pestat] mean and std.dev: (2367.06, 1972.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 312, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (288.44, 118.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 100, #reads = 1083140 mem = 536883537 peak = 799211520 [M::process] read 700584 sequences (100000164 bp) in 3.206 CPU sec, 3.824 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 288827, 21, 111) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 299, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1755) [M::mem_pestat] mean and std.dev: (372.53, 325.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (266.93, 135.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1118, 1535, 2440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5084) [M::mem_pestat] mean and std.dev: (1427.05, 868.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 331, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (311.37, 151.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700640 reads in 219.377 CPU sec, 7.114 real sec [M::process] read 700452 sequences (100000081 bp) in 2.382 CPU sec, 2.517 real sec... [M::mem_process_seqs] Processed 700670 reads in 148.862 CPU sec, 4.780 real sec sorting block 101, #reads = 1083747 mem = 536878616 peak = 799211520 [M::process] read 700612 sequences (100000220 bp) in 3.122 CPU sec, 3.523 real sec... [M::process] read 290854 sequences (41385908 bp) in 1.306 CPU sec, 1.310 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (108, 288715, 38, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 279, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1479) [M::mem_pestat] mean and std.dev: (308.07, 200.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (267.66, 136.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (377, 392, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2744) [M::mem_pestat] mean and std.dev: (640.39, 536.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 308, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (309.62, 130.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700452 reads in 127.110 CPU sec, 4.059 real sec sorting block 102, #reads = 1084286 mem = 536877982 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 288630, 15, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1864) [M::mem_pestat] mean and std.dev: (410.17, 369.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (266.99, 135.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (676, 839, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2074) [M::mem_pestat] mean and std.dev: (695.92, 282.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 253, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (264.16, 103.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (94, 288947, 21, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 275, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (300.41, 172.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (268.36, 137.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (324, 495, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1719) [M::mem_pestat] mean and std.dev: (417.59, 218.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 258, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (280.52, 143.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700584 reads in 336.203 CPU sec, 10.930 real sec [M::mem_process_seqs] Processed 700612 reads in 166.124 CPU sec, 5.457 real sec sorting block 103, #reads = 1082104 mem = 536878452 peak = 799211520 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 119660, 13, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 278, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558) [M::mem_pestat] mean and std.dev: (357.33, 301.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 233, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (260.87, 133.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (132, 1591, 2685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7791) [M::mem_pestat] mean and std.dev: (1572.62, 1590.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 271, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (297.60, 182.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 290854 reads in 53.480 CPU sec, 1.857 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 112995462 overall: 69652254720 mem 20542.254 user 940.775 sys 731.771 real sorting block 104, #reads = 1029458 mem = 510463393 peak = 799211520 [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_6TL-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1136_6TL-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_6TL-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_6TL-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_6TL-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 731.829 sec; CPU: 21483.053 sec uniq header count 1 merging 105 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 6072799472 output reads: 113766675 bam_mem_sort: 105 calls 92.490 user 1.996 sys 95.444 real bam_write: 105 calls 63.100 user 4.764 sys 100.584 real execute: 1 calls 43.352 user 4.964 sys 789.542 real merge_files: 1 calls 43.342 user 4.924 sys 56.543 real parse_chunk: 1680 calls 78.501 user 23.280 sys 116.668 real read_chunk: 4541 calls 56.699 user 28.955 sys 631.616 real sort_block: 1 calls 41.709 user 27.659 sys 607.838 real write_chunk: 2859 calls 42.756 user 4.583 sys 55.308 real overall: 2749575168 mem 1778.931 user 99.627 sys 789.562 real ls: cannot access '*.cram': No such file or directory