.command.err - Workdir - DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L008;0004)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8e/8632e6651fa7bca668982a6bf56989/.command.err
Size: 138.3 KB
Task: DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L008;0004)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
Received only 30 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 1554.401 CPU sec, 893.988 real sec
[M::process] read 698400 sequences (100000029 bp) in 0.948 CPU sec, 0.957 real sec...
[M::process] read 696448 sequences (100000053 bp) in 1.263 CPU sec, 1.301 real sec...
[M::process] read 698906 sequences (100000010 bp) in 1.230 CPU sec, 1.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291298, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.59, 122.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699126 sequences (100000178 bp) in 1.179 CPU sec, 1.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290305, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (579, 767, 1153)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2301)
[M::mem_pestat] mean and std.dev: (771.58, 574.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3070)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (260.12, 125.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291591, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (263, 263, 808)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898)
[M::mem_pestat] mean and std.dev: (554.36, 505.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2576)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.04, 122.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698400 reads in 201.521 CPU sec, 6.615 real sec
[M::mem_process_seqs] Processed 696448 reads in 132.577 CPU sec, 4.354 real sec
[M::mem_process_seqs] Processed 698906 reads in 143.619 CPU sec, 4.727 real sec
sorting block 0, #reads = 1094026 mem = 536878274 peak = 597032960
[M::process] read 698328 sequences (100000171 bp) in 1.770 CPU sec, 1.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291849, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (109, 221, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (214.78, 114.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.53, 121.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696396 sequences (100000265 bp) in 1.296 CPU sec, 1.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290793, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.46, 123.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699126 reads in 125.119 CPU sec, 3.981 real sec
sorting block 1, #reads = 1094074 mem = 536878399 peak = 904593408
[M::mem_process_seqs] Processed 698328 reads in 78.853 CPU sec, 2.483 real sec
[M::process] read 698946 sequences (100000056 bp) in 1.326 CPU sec, 1.347 real sec...
sorting block 2, #reads = 1093866 mem = 536879514 peak = 904593408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290857, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693)
[M::mem_pestat] mean and std.dev: (259.82, 126.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698096 sequences (100000174 bp) in 1.711 CPU sec, 1.796 real sec...
[M::process] read 697244 sequences (100000230 bp) in 1.250 CPU sec, 1.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290888, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 282, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (95, 490)
[M::mem_pestat] mean and std.dev: (278.60, 51.91)
[M::mem_pestat] low and high boundaries for proper pairs: (16, 569)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.85, 121.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696396 reads in 124.199 CPU sec, 3.953 real sec
[M::mem_process_seqs] Processed 698946 reads in 78.240 CPU sec, 2.487 real sec
sorting block 3, #reads = 1093974 mem = 536883382 peak = 904593408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290846, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.63, 122.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 217, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (282.50, 220.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RR
[M::process] read 695956 sequences (100000277 bp) in 1.839 CPU sec, 1.938 real sec...
[M::process] read 697140 sequences (100000196 bp) in 1.280 CPU sec, 1.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290744, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 285, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (326.55, 148.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.58, 121.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698096 reads in 125.263 CPU sec, 3.987 real sec
[M::mem_process_seqs] Processed 697244 reads in 78.757 CPU sec, 2.511 real sec
sorting block 4, #reads = 1094139 mem = 536878145 peak = 904593408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290104, 8, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.33, 122.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 450, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (306.89, 145.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RR
[M::process] read 696610 sequences (100000111 bp) in 1.861 CPU sec, 1.968 real sec...
[M::process] read 696822 sequences (100000231 bp) in 1.301 CPU sec, 1.310 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290190, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.81, 120.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695956 reads in 127.994 CPU sec, 4.084 real sec
sorting block 5, #reads = 1093243 mem = 536879079 peak = 904593408
[M::mem_process_seqs] Processed 697140 reads in 79.684 CPU sec, 2.536 real sec
sorting block 6, #reads = 1092907 mem = 536881259 peak = 904593408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290203, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 189, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (70, 310)
[M::mem_pestat] mean and std.dev: (177.10, 57.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 408)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.56, 121.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 694318 sequences (100000019 bp) in 2.010 CPU sec, 2.119 real sec...
[M::process] read 696678 sequences (100000268 bp) in 1.340 CPU sec, 1.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290084, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 270, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (290.00, 137.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.03, 119.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696610 reads in 128.718 CPU sec, 4.099 real sec
[M::mem_process_seqs] Processed 696822 reads in 80.111 CPU sec, 2.546 real sec
sorting block 7, #reads = 1091699 mem = 536884205 peak = 904593408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289431, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 280, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532)
[M::mem_pestat] mean and std.dev: (222.56, 63.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 655)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 237, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (259.93, 122.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695364 sequences (100000178 bp) in 2.107 CPU sec, 2.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290450, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.43, 119.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 322, 1514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4066)
[M::mem_pestat] mean and std.dev: (651.00, 566.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5342)
[M::mem_pestat] skip orientation RR
[M::process] read 696652 sequences (100000138 bp) in 1.370 CPU sec, 1.373 real sec...
[M::mem_process_seqs] Processed 694318 reads in 128.375 CPU sec, 4.094 real sec
[M::mem_process_seqs] Processed 696678 reads in 80.883 CPU sec, 2.563 real sec
sorting block 8, #reads = 1092431 mem = 536878849 peak = 904593408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289751, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.63, 120.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (501, 501, 673)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (157, 1017)
[M::mem_pestat] mean and std.dev: (477.78, 175.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RR
[M::process] read 695974 sequences (100000242 bp) in 2.278 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290175, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.74, 121.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695364 reads in 129.013 CPU sec, 4.117 real sec
[M::process] read 697936 sequences (100000293 bp) in 1.431 CPU sec, 1.446 real sec...
[M::mem_process_seqs] Processed 696652 reads in 81.942 CPU sec, 2.596 real sec
sorting block 9, #reads = 1092520 mem = 536880669 peak = 904593408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290046, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (255.94, 122.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697890 sequences (100000153 bp) in 2.219 CPU sec, 2.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290568, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (247, 343, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (333.27, 138.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.44, 121.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1295)
[M::mem_pestat] mean and std.dev: (376.15, 302.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1675)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698158 sequences (100000121 bp) in 1.377 CPU sec, 1.379 real sec...
[M::mem_process_seqs] Processed 695974 reads in 135.160 CPU sec, 4.315 real sec
sorting block 10, #reads = 1092257 mem = 536879746 peak = 904593408
[M::mem_process_seqs] Processed 697936 reads in 85.098 CPU sec, 2.707 real sec
sorting block 11, #reads = 1092439 mem = 536884058 peak = 949657600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290875, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.14, 121.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698244 sequences (100000052 bp) in 2.197 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290764, 7, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 234, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (279.30, 193.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 231, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (255.35, 123.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698680 sequences (100000227 bp) in 1.400 CPU sec, 1.400 real sec...
[M::mem_process_seqs] Processed 697890 reads in 134.575 CPU sec, 4.297 real sec
[M::mem_process_seqs] Processed 698158 reads in 83.987 CPU sec, 2.668 real sec
sorting block 12, #reads = 1094823 mem = 536879968 peak = 949657600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290755, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (254.86, 123.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696900 sequences (100000204 bp) in 2.168 CPU sec, 2.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291056, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.08, 122.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697878 sequences (100000152 bp) in 1.400 CPU sec, 1.393 real sec...
[M::mem_process_seqs] Processed 698244 reads in 132.249 CPU sec, 4.219 real sec
sorting block 13, #reads = 1095009 mem = 536879030 peak = 949657600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290496, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (257.91, 124.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 869)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697926 sequences (100000150 bp) in 1.570 CPU sec, 1.574 real sec...
[M::mem_process_seqs] Processed 696900 reads in 71.362 CPU sec, 2.445 real sec
[M::mem_process_seqs] Processed 698680 reads in 171.366 CPU sec, 5.632 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291000, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.10, 121.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1094252 mem = 536881117 peak = 949657600
[M::process] read 697376 sequences (100000154 bp) in 2.088 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291363, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.18, 122.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697878 reads in 131.770 CPU sec, 4.184 real sec
[M::mem_process_seqs] Processed 697926 reads in 77.673 CPU sec, 2.449 real sec
[M::process] read 694918 sequences (100000027 bp) in 1.686 CPU sec, 1.761 real sec...
sorting block 15, #reads = 1094559 mem = 536877431 peak = 949657600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290830, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.27, 122.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 240, 666)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1590)
[M::mem_pestat] mean and std.dev: (277.88, 154.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2052)
[M::mem_pestat] skip orientation RR
[M::process] read 696554 sequences (100000098 bp) in 1.847 CPU sec, 2.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290472, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 239, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (261.83, 124.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697376 reads in 122.125 CPU sec, 3.890 real sec
[M::mem_process_seqs] Processed 694918 reads in 77.157 CPU sec, 2.433 real sec
[M::process] read 697016 sequences (100000110 bp) in 1.621 CPU sec, 1.661 real sec...
sorting block 16, #reads = 1094214 mem = 536880459 peak = 949657600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290044, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.46, 120.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697194 sequences (100000053 bp) in 2.124 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290574, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 305, 732)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844)
[M::mem_pestat] mean and std.dev: (406.60, 242.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2400)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.35, 121.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696554 reads in 122.349 CPU sec, 3.892 real sec
sorting block 17, #reads = 1093203 mem = 536878946 peak = 949657600
[M::mem_process_seqs] Processed 697016 reads in 77.806 CPU sec, 2.453 real sec
[M::process] read 695822 sequences (100000224 bp) in 1.592 CPU sec, 1.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290659, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.44, 120.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 332, 689)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653)
[M::mem_pestat] mean and std.dev: (442.20, 354.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2135)
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1093092 mem = 536875788 peak = 978362368
[M::process] read 695732 sequences (100000264 bp) in 2.116 CPU sec, 2.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290345, 11, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.33, 120.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 607, 2394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6908)
[M::mem_pestat] mean and std.dev: (1427.45, 1578.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9165)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 697194 reads in 122.951 CPU sec, 3.910 real sec
[M::mem_process_seqs] Processed 695822 reads in 78.442 CPU sec, 2.467 real sec
[M::process] read 696692 sequences (100000152 bp) in 1.531 CPU sec, 1.570 real sec...
sorting block 19, #reads = 1092598 mem = 536878334 peak = 978362368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290014, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.16, 120.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696668 sequences (100000120 bp) in 2.202 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290498, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.53, 119.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695732 reads in 123.459 CPU sec, 3.928 real sec
[M::mem_process_seqs] Processed 696692 reads in 79.425 CPU sec, 2.502 real sec
[M::process] read 697364 sequences (100000005 bp) in 1.635 CPU sec, 1.682 real sec...
sorting block 20, #reads = 1092979 mem = 536876980 peak = 978362368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290081, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.70, 120.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697802 sequences (100000191 bp) in 2.315 CPU sec, 2.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290675, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.35, 120.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696668 reads in 126.084 CPU sec, 4.010 real sec
[M::mem_process_seqs] Processed 697364 reads in 81.038 CPU sec, 2.554 real sec
sorting block 21, #reads = 1092191 mem = 536878558 peak = 978362368
[M::process] read 699216 sequences (100000115 bp) in 2.301 CPU sec, 2.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290260, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.38, 120.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 22, #reads = 1091567 mem = 536883677 peak = 978362368
[M::process] read 699068 sequences (100000209 bp) in 2.429 CPU sec, 2.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291445, 10, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.20, 118.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (338, 602, 810)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1754)
[M::mem_pestat] mean and std.dev: (587.80, 212.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2226)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 697802 reads in 131.977 CPU sec, 4.219 real sec
[M::mem_process_seqs] Processed 699216 reads in 84.674 CPU sec, 2.681 real sec
[M::process] read 698070 sequences (100000057 bp) in 2.512 CPU sec, 2.660 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290984, 16, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.49, 119.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (97, 580, 702)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1912)
[M::mem_pestat] mean and std.dev: (614.25, 518.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2687)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 23, #reads = 1092404 mem = 536880037 peak = 978362368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291067, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 514, 2973)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8653)
[M::mem_pestat] mean and std.dev: (1325.30, 1350.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11493)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.55, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 215, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (241.00, 108.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 696646 sequences (100000150 bp) in 2.295 CPU sec, 2.404 real sec...
[M::mem_process_seqs] Processed 699068 reads in 131.813 CPU sec, 4.225 real sec
[M::mem_process_seqs] Processed 698070 reads in 83.600 CPU sec, 2.658 real sec
sorting block 24, #reads = 1093081 mem = 536879010 peak = 978362368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290645, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 236, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (260.40, 125.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697158 sequences (100000123 bp) in 2.548 CPU sec, 2.723 real sec...
[M::mem_process_seqs] Processed 696646 reads in 71.168 CPU sec, 2.360 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290486, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 197, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (206.00, 84.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (256.60, 122.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 265, 3395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9685)
[M::mem_pestat] mean and std.dev: (1461.90, 1955.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12830)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697158 reads in 70.598 CPU sec, 2.334 real sec
sorting block 25, #reads = 1093057 mem = 536883131 peak = 978362368
[M::process] read 698528 sequences (100000189 bp) in 2.843 CPU sec, 3.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291768, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.20, 121.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698528 reads in 70.579 CPU sec, 2.327 real sec
sorting block 26, #reads = 1094602 mem = 536876959 peak = 978362368
[M::process] read 697576 sequences (100000001 bp) in 2.649 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291026, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.92, 121.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697576 reads in 70.216 CPU sec, 2.284 real sec
[M::process] read 697724 sequences (100000102 bp) in 2.848 CPU sec, 3.079 real sec...
sorting block 27, #reads = 1095391 mem = 536880846 peak = 978362368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291048, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 314, 1317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3611)
[M::mem_pestat] mean and std.dev: (499.00, 470.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4758)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.09, 121.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697016 sequences (100000263 bp) in 2.041 CPU sec, 2.134 real sec...
[M::process] read 694862 sequences (100000027 bp) in 1.460 CPU sec, 1.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290748, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (256.36, 123.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 907, 946)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2346)
[M::mem_pestat] mean and std.dev: (509.50, 333.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3046)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697016 reads in 69.548 CPU sec, 2.182 real sec
[M::process] read 696856 sequences (100000151 bp) in 1.537 CPU sec, 1.590 real sec...
[M::mem_process_seqs] Processed 697724 reads in 170.807 CPU sec, 5.436 real sec
sorting block 28, #reads = 1093672 mem = 536877003 peak = 978362368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 289536, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 239, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (261.92, 124.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696618 sequences (100000117 bp) in 2.073 CPU sec, 2.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290750, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 479, 765)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1827)
[M::mem_pestat] mean and std.dev: (546.36, 405.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2358)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.53, 121.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 227, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (77, 377)
[M::mem_pestat] mean and std.dev: (212.30, 36.54)
[M::mem_pestat] low and high boundaries for proper pairs: (17, 437)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 694862 reads in 139.294 CPU sec, 4.408 real sec
[M::mem_process_seqs] Processed 696856 reads in 81.936 CPU sec, 2.590 real sec
[M::process] read 696716 sequences (100000066 bp) in 1.674 CPU sec, 1.747 real sec...
sorting block 29, #reads = 1092461 mem = 536882605 peak = 978362368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290302, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (281, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (47, 632)
[M::mem_pestat] mean and std.dev: (259.67, 77.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 749)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.46, 121.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697136 sequences (100000185 bp) in 1.952 CPU sec, 2.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290679, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.23, 120.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697224 sequences (100000013 bp) in 1.270 CPU sec, 1.266 real sec...
[M::mem_process_seqs] Processed 696618 reads in 129.131 CPU sec, 4.101 real sec
sorting block 30, #reads = 1093445 mem = 536877062 peak = 978362368
[M::mem_process_seqs] Processed 696716 reads in 81.255 CPU sec, 2.581 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290479, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.23, 118.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 847, 3020)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8598)
[M::mem_pestat] mean and std.dev: (1703.18, 1965.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11387)
[M::mem_pestat] skip orientation RR
sorting block 31, #reads = 1092986 mem = 536878231 peak = 1005051904
[M::process] read 696698 sequences (100000210 bp) in 2.063 CPU sec, 2.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290117, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.66, 119.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 272, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563)
[M::mem_pestat] mean and std.dev: (258.31, 72.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 684)
[M::mem_pestat] skip orientation RR
[M::process] read 696892 sequences (100000196 bp) in 1.330 CPU sec, 1.335 real sec...
[M::mem_process_seqs] Processed 697136 reads in 128.208 CPU sec, 4.072 real sec
[M::mem_process_seqs] Processed 697224 reads in 81.090 CPU sec, 2.569 real sec
sorting block 32, #reads = 1093730 mem = 536879909 peak = 1005051904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290398, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.49, 119.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696860 sequences (100000236 bp) in 1.821 CPU sec, 1.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290262, 7, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 188, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405)
[M::mem_pestat] mean and std.dev: (182.71, 64.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 493)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.90, 120.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697670 sequences (100000047 bp) in 1.320 CPU sec, 1.313 real sec...
[M::mem_process_seqs] Processed 696698 reads in 127.081 CPU sec, 4.044 real sec
[M::mem_process_seqs] Processed 696892 reads in 80.927 CPU sec, 2.563 real sec
sorting block 33, #reads = 1092715 mem = 536880897 peak = 1005051904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290243, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.87, 120.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696024 sequences (100000293 bp) in 2.228 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290078, 3, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (252.40, 91.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.70, 119.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (368, 468, 688)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328)
[M::mem_pestat] mean and std.dev: (559.08, 281.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1687)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696860 reads in 130.683 CPU sec, 4.159 real sec
[M::process] read 696374 sequences (100000291 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_process_seqs] Processed 697670 reads in 83.320 CPU sec, 2.641 real sec
sorting block 34, #reads = 1093045 mem = 536877839 peak = 1005051904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290019, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (257.05, 121.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697470 sequences (100000240 bp) in 2.266 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290439, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (256.62, 121.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 165, 226)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 354)
[M::mem_pestat] mean and std.dev: (177.08, 42.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 418)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696024 reads in 124.098 CPU sec, 3.943 real sec
[M::process] read 695040 sequences (100000294 bp) in 1.445 CPU sec, 1.456 real sec...
sorting block 35, #reads = 1092410 mem = 536878933 peak = 1005051904
[M::mem_process_seqs] Processed 696374 reads in 79.027 CPU sec, 2.492 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290727, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.77, 119.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1092860 mem = 536878786 peak = 1027674112
[M::process] read 697906 sequences (100000001 bp) in 2.297 CPU sec, 2.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290247, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 240, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (263.73, 126.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697470 reads in 124.292 CPU sec, 3.953 real sec
[M::mem_process_seqs] Processed 695040 reads in 79.564 CPU sec, 2.511 real sec
[M::process] read 698672 sequences (100000182 bp) in 1.614 CPU sec, 1.646 real sec...
sorting block 37, #reads = 1093548 mem = 536878169 peak = 1027674112
[M::process] read 698456 sequences (100000100 bp) in 2.115 CPU sec, 2.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291017, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.43, 121.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698672 reads in 70.567 CPU sec, 2.219 real sec
[M::process] read 698300 sequences (100000264 bp) in 1.461 CPU sec, 1.466 real sec...
sorting block 38, #reads = 1094554 mem = 536876397 peak = 1027674112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291212, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.76, 122.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290855, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.55, 120.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 283, 827)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2143)
[M::mem_pestat] mean and std.dev: (483.33, 472.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2801)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697906 reads in 195.754 CPU sec, 6.213 real sec
[M::mem_process_seqs] Processed 698456 reads in 93.513 CPU sec, 2.963 real sec
[M::process] read 696692 sequences (100000092 bp) in 1.901 CPU sec, 1.936 real sec...
sorting block 39, #reads = 1093683 mem = 536879738 peak = 1027674112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291322, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.09, 121.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696694 sequences (100000210 bp) in 2.286 CPU sec, 2.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290789, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 293, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 477)
[M::mem_pestat] mean and std.dev: (242.67, 47.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 565)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (257.50, 123.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698300 reads in 127.838 CPU sec, 4.066 real sec
[M::mem_process_seqs] Processed 696692 reads in 81.683 CPU sec, 2.579 real sec
[M::process] read 697452 sequences (100000003 bp) in 1.534 CPU sec, 1.571 real sec...
sorting block 40, #reads = 1093337 mem = 536879855 peak = 1027674112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290364, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 184, 211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 323)
[M::mem_pestat] mean and std.dev: (186.30, 43.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 379)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (257.44, 123.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695974 sequences (100000044 bp) in 2.049 CPU sec, 2.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290994, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.69, 122.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696694 reads in 127.543 CPU sec, 4.063 real sec
[M::process] read 696820 sequences (100000038 bp) in 1.496 CPU sec, 1.505 real sec...
[M::mem_process_seqs] Processed 697452 reads in 81.461 CPU sec, 2.574 real sec
sorting block 41, #reads = 1093364 mem = 536878624 peak = 1027674112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290586, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 244, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (268.67, 174.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (258.49, 123.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697208 sequences (100000010 bp) in 2.017 CPU sec, 2.140 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290252, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.33, 120.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 221, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (244.58, 106.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695974 reads in 128.862 CPU sec, 4.118 real sec
sorting block 42, #reads = 1093326 mem = 536878200 peak = 1027674112
[M::mem_process_seqs] Processed 696820 reads in 82.912 CPU sec, 2.615 real sec
[M::process] read 697156 sequences (100000223 bp) in 1.597 CPU sec, 1.631 real sec...
sorting block 43, #reads = 1094144 mem = 536877443 peak = 1073262592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290726, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.21, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696902 sequences (100000283 bp) in 2.134 CPU sec, 2.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290090, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.28, 119.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696668 sequences (100000294 bp) in 1.380 CPU sec, 1.385 real sec...
[M::mem_process_seqs] Processed 697208 reads in 130.519 CPU sec, 4.148 real sec
[M::mem_process_seqs] Processed 697156 reads in 83.220 CPU sec, 2.630 real sec
sorting block 44, #reads = 1092339 mem = 536882321 peak = 1073262592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290086, 8, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 191, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (196.58, 80.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.18, 119.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700162 sequences (100000015 bp) in 2.256 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289917, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.24, 119.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696902 reads in 129.949 CPU sec, 4.148 real sec
[M::mem_process_seqs] Processed 696668 reads in 83.552 CPU sec, 2.637 real sec
[M::process] read 702008 sequences (100000052 bp) in 1.754 CPU sec, 1.816 real sec...
sorting block 45, #reads = 1092051 mem = 536879261 peak = 1073262592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291433, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (243.58, 116.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (228.90, 88.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RR
[M::process] read 701920 sequences (100000129 bp) in 2.078 CPU sec, 2.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292357, 8, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.29, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 316, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (299.73, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation RR
[M::process] read 702774 sequences (100000269 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_process_seqs] Processed 700162 reads in 136.457 CPU sec, 4.335 real sec
sorting block 46, #reads = 1091641 mem = 536880122 peak = 1073262592
[M::mem_process_seqs] Processed 702008 reads in 86.459 CPU sec, 2.733 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292094, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (249, 465, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1509)
[M::mem_pestat] mean and std.dev: (379.00, 187.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1929)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.49, 120.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 47, #reads = 1092985 mem = 536881222 peak = 1073262592
[M::process] read 702584 sequences (100000068 bp) in 2.524 CPU sec, 2.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292775, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.26, 117.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701920 reads in 134.592 CPU sec, 4.277 real sec
[M::mem_process_seqs] Processed 702774 reads in 85.506 CPU sec, 2.696 real sec
[M::process] read 699300 sequences (100000295 bp) in 1.608 CPU sec, 1.634 real sec...
sorting block 48, #reads = 1093545 mem = 536881732 peak = 1073262592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292729, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.85, 116.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 1182, 1182)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120)
[M::mem_pestat] mean and std.dev: (687.45, 512.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4089)
[M::mem_pestat] skip orientation RR
[M::process] read 702354 sequences (100000057 bp) in 2.283 CPU sec, 2.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291522, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (251.83, 123.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702584 reads in 134.410 CPU sec, 4.274 real sec
[M::process] read 701784 sequences (100000248 bp) in 1.486 CPU sec, 1.489 real sec...
[M::mem_process_seqs] Processed 699300 reads in 85.459 CPU sec, 2.700 real sec
sorting block 49, #reads = 1094520 mem = 536878183 peak = 1073262592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292834, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.96, 117.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698716 sequences (100000004 bp) in 2.318 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292676, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (244.70, 119.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 339, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204)
[M::mem_pestat] mean and std.dev: (310.27, 137.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1551)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702354 reads in 135.775 CPU sec, 4.318 real sec
[M::process] read 698820 sequences (100000006 bp) in 1.449 CPU sec, 1.453 real sec...
[M::mem_process_seqs] Processed 701784 reads in 85.537 CPU sec, 2.709 real sec
sorting block 50, #reads = 1092840 mem = 536876452 peak = 1073262592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291455, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.60, 123.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697828 sequences (100000022 bp) in 2.269 CPU sec, 2.398 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291420, 6, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 1191, 8592)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25370)
[M::mem_pestat] mean and std.dev: (2546.62, 3374.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 33759)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.88, 123.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697816 sequences (100000114 bp) in 1.470 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 698716 reads in 134.854 CPU sec, 4.281 real sec
sorting block 51, #reads = 1093969 mem = 536882012 peak = 1073262592
[M::mem_process_seqs] Processed 698820 reads in 85.867 CPU sec, 2.713 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290561, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.46, 122.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 52, #reads = 1092135 mem = 536880062 peak = 1073262592
[M::process] read 697124 sequences (100000215 bp) in 2.437 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290820, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.49, 120.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697828 reads in 135.869 CPU sec, 4.321 real sec
[M::process] read 697380 sequences (100000295 bp) in 1.569 CPU sec, 1.594 real sec...
[M::mem_process_seqs] Processed 697816 reads in 86.459 CPU sec, 2.726 real sec
sorting block 53, #reads = 1091192 mem = 536879370 peak = 1073262592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290747, 8, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 249, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172)
[M::mem_pestat] mean and std.dev: (350.60, 267.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1506)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.90, 123.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697318 sequences (100000160 bp) in 2.280 CPU sec, 2.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290462, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (253.02, 122.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (285, 396, 1133)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2829)
[M::mem_pestat] mean and std.dev: (672.10, 520.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3677)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697124 reads in 137.028 CPU sec, 4.360 real sec
[M::process] read 697186 sequences (100000214 bp) in 1.468 CPU sec, 1.472 real sec...
[M::mem_process_seqs] Processed 697380 reads in 87.465 CPU sec, 2.765 real sec
sorting block 54, #reads = 1090542 mem = 536879858 peak = 1073262592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290767, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 328, 700)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1680)
[M::mem_pestat] mean and std.dev: (468.70, 347.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2170)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.38, 121.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697404 sequences (100000073 bp) in 2.243 CPU sec, 2.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290402, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.64, 121.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697318 reads in 135.706 CPU sec, 4.330 real sec
[M::mem_process_seqs] Processed 697186 reads in 86.580 CPU sec, 2.730 real sec
sorting block 55, #reads = 1089805 mem = 536879838 peak = 1073262592
[M::process] read 703618 sequences (100000140 bp) in 1.709 CPU sec, 1.754 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290811, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (316, 335, 829)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1855)
[M::mem_pestat] mean and std.dev: (365.38, 204.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2368)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.21, 120.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 56, #reads = 1090921 mem = 536877497 peak = 1089155072
[M::process] read 704964 sequences (100000088 bp) in 2.261 CPU sec, 2.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292941, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.47, 116.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703562 sequences (100000038 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 697404 reads in 142.433 CPU sec, 4.525 real sec
[M::mem_process_seqs] Processed 703618 reads in 93.857 CPU sec, 2.970 real sec
sorting block 57, #reads = 1089858 mem = 536878556 peak = 1089155072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293496, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 212, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (237.84, 115.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703022 sequences (100000186 bp) in 2.422 CPU sec, 2.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292246, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (239.94, 115.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701382 sequences (100000021 bp) in 1.480 CPU sec, 1.476 real sec...
[M::mem_process_seqs] Processed 704964 reads in 151.969 CPU sec, 4.832 real sec
[M::mem_process_seqs] Processed 703562 reads in 95.170 CPU sec, 3.014 real sec
sorting block 58, #reads = 1087600 mem = 536876910 peak = 1089155072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292698, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.28, 115.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (84, 103, 121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 195)
[M::mem_pestat] mean and std.dev: (84.00, 26.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 232)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 702242 sequences (100000118 bp) in 2.364 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292648, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.48, 118.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702652 sequences (100000112 bp) in 1.460 CPU sec, 1.457 real sec...
[M::mem_process_seqs] Processed 703022 reads in 144.581 CPU sec, 4.591 real sec
[M::mem_process_seqs] Processed 701382 reads in 91.525 CPU sec, 2.902 real sec
sorting block 59, #reads = 1089334 mem = 536879126 peak = 1089155072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292691, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.58, 116.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 369, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (328.50, 188.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RR
[M::process] read 701312 sequences (100000197 bp) in 2.376 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292530, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.12, 116.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698762 sequences (100000281 bp) in 1.440 CPU sec, 1.442 real sec...
[M::mem_process_seqs] Processed 702242 reads in 139.534 CPU sec, 4.437 real sec
sorting block 60, #reads = 1090415 mem = 536879480 peak = 1089155072
[M::mem_process_seqs] Processed 702652 reads in 88.911 CPU sec, 2.810 real sec
sorting block 61, #reads = 1093313 mem = 536879194 peak = 1100951552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292686, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.63, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699574 sequences (100000040 bp) in 2.318 CPU sec, 2.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291938, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 767, 879)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131)
[M::mem_pestat] mean and std.dev: (557.40, 297.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2757)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.46, 124.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698492 sequences (100000192 bp) in 1.450 CPU sec, 1.445 real sec...
[M::mem_process_seqs] Processed 701312 reads in 139.719 CPU sec, 4.436 real sec
[M::mem_process_seqs] Processed 698762 reads in 87.598 CPU sec, 2.773 real sec
sorting block 62, #reads = 1091381 mem = 536877524 peak = 1100951552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291557, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.15, 121.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697876 sequences (100000300 bp) in 2.491 CPU sec, 2.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290872, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.01, 121.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696854 sequences (100000289 bp) in 1.430 CPU sec, 1.423 real sec...
[M::mem_process_seqs] Processed 699574 reads in 137.924 CPU sec, 4.377 real sec
[M::mem_process_seqs] Processed 698492 reads in 87.928 CPU sec, 2.783 real sec
sorting block 63, #reads = 1090954 mem = 536879967 peak = 1100951552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291346, 9, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 352, 1122)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2964)
[M::mem_pestat] mean and std.dev: (371.27, 320.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3885)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.34, 122.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698338 sequences (100000178 bp) in 2.363 CPU sec, 2.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290804, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.68, 124.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698114 sequences (100000264 bp) in 1.420 CPU sec, 1.426 real sec...
[M::mem_process_seqs] Processed 697876 reads in 135.768 CPU sec, 4.310 real sec
sorting block 64, #reads = 1090555 mem = 536878236 peak = 1100951552
[M::mem_process_seqs] Processed 696854 reads in 86.038 CPU sec, 2.721 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290958, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.97, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 65, #reads = 1090027 mem = 536879385 peak = 1100951552
[M::process] read 698602 sequences (100000278 bp) in 2.427 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290673, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 595, 713)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1619)
[M::mem_pestat] mean and std.dev: (533.69, 316.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2072)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.63, 121.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702052 sequences (100000282 bp) in 1.480 CPU sec, 1.480 real sec...
[M::mem_process_seqs] Processed 698338 reads in 136.784 CPU sec, 4.338 real sec
[M::mem_process_seqs] Processed 698114 reads in 86.966 CPU sec, 2.749 real sec
sorting block 66, #reads = 1091631 mem = 536879204 peak = 1100951552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290917, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.85, 120.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702546 sequences (100000047 bp) in 2.347 CPU sec, 2.436 real sec...
[M::process] read 701334 sequences (100000127 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291767, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.87, 116.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 470, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504)
[M::mem_pestat] mean and std.dev: (374.44, 236.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1954)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698602 reads in 146.845 CPU sec, 4.665 real sec
[M::mem_process_seqs] Processed 702052 reads in 96.510 CPU sec, 3.065 real sec
sorting block 67, #reads = 1089352 mem = 536877691 peak = 1100951552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292405, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (242.31, 117.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701284 sequences (100000119 bp) in 2.469 CPU sec, 2.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292464, 8, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 384, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215)
[M::mem_pestat] mean and std.dev: (354.91, 183.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1556)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.93, 118.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (390, 406, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1530)
[M::mem_pestat] mean and std.dev: (447.09, 228.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1910)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702546 reads in 133.280 CPU sec, 4.229 real sec
[M::process] read 698274 sequences (100000180 bp) in 1.447 CPU sec, 1.455 real sec...
[M::mem_process_seqs] Processed 701334 reads in 84.110 CPU sec, 2.658 real sec
sorting block 68, #reads = 1086882 mem = 536876785 peak = 1100951552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292402, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (246.54, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1094380 mem = 536879413 peak = 1100951552
[M::process] read 701628 sequences (100000062 bp) in 2.330 CPU sec, 2.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291347, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 230, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (254.97, 123.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701284 reads in 132.492 CPU sec, 4.205 real sec
[M::process] read 702060 sequences (100000134 bp) in 1.460 CPU sec, 1.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292198, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.57, 116.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 179, 1964)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5654)
[M::mem_pestat] mean and std.dev: (922.46, 1489.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7499)
[M::mem_pestat] skip orientation RR
[M::process] read 701450 sequences (100000222 bp) in 1.696 CPU sec, 1.696 real sec...
[M::mem_process_seqs] Processed 701628 reads in 79.923 CPU sec, 2.822 real sec
sorting block 70, #reads = 1093909 mem = 536880800 peak = 1100951552
[M::mem_process_seqs] Processed 698274 reads in 187.007 CPU sec, 6.220 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292434, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.66, 115.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700980 sequences (100000094 bp) in 2.378 CPU sec, 2.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292456, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.29, 117.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (115, 302, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (212.88, 109.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702060 reads in 147.285 CPU sec, 4.689 real sec
sorting block 71, #reads = 1092665 mem = 536877007 peak = 1100951552
[M::mem_process_seqs] Processed 701450 reads in 85.640 CPU sec, 2.706 real sec
[M::process] read 698504 sequences (100000013 bp) in 1.900 CPU sec, 2.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292231, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 240, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (207.30, 74.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.76, 118.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 72, #reads = 1094722 mem = 536882888 peak = 1100951552
[M::process] read 700088 sequences (100000113 bp) in 2.192 CPU sec, 2.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291336, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (253.77, 123.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 237, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (297.64, 119.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700980 reads in 134.912 CPU sec, 4.294 real sec
[M::mem_process_seqs] Processed 698504 reads in 85.793 CPU sec, 2.713 real sec
[M::process] read 699652 sequences (100000250 bp) in 1.692 CPU sec, 1.740 real sec...
sorting block 73, #reads = 1092637 mem = 536883966 peak = 1100951552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291617, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (246.96, 118.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (346, 597, 1053)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2467)
[M::mem_pestat] mean and std.dev: (732.45, 564.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3174)
[M::mem_pestat] skip orientation RR
[M::process] read 698968 sequences (100000076 bp) in 2.254 CPU sec, 2.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291626, 10, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 379, 1157)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3021)
[M::mem_pestat] mean and std.dev: (598.42, 621.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3953)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.75, 119.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 170, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425)
[M::mem_pestat] mean and std.dev: (161.44, 70.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 510)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 700088 reads in 134.746 CPU sec, 4.291 real sec
[M::mem_process_seqs] Processed 699652 reads in 85.772 CPU sec, 2.702 real sec
[M::process] read 698828 sequences (100000112 bp) in 1.565 CPU sec, 1.592 real sec...
sorting block 74, #reads = 1093425 mem = 536879282 peak = 1100951552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291129, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.17, 121.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699118 sequences (100000136 bp) in 2.267 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290893, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 370, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (302.82, 216.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.80, 120.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699430 sequences (100000115 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_process_seqs] Processed 698968 reads in 136.155 CPU sec, 4.328 real sec
sorting block 75, #reads = 1092489 mem = 536880856 peak = 1100951552
[M::mem_process_seqs] Processed 698828 reads in 86.581 CPU sec, 2.739 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291706, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (248.99, 120.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 179, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132)
[M::mem_pestat] mean and std.dev: (295.64, 214.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1466)
[M::mem_pestat] skip orientation RR
sorting block 76, #reads = 1091201 mem = 536878788 peak = 1100951552
[M::process] read 699940 sequences (100000051 bp) in 2.224 CPU sec, 2.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291514, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.27, 120.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 239, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 463)
[M::mem_pestat] mean and std.dev: (235.89, 54.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 549)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699118 reads in 138.088 CPU sec, 4.393 real sec
[M::process] read 703852 sequences (100000051 bp) in 1.596 CPU sec, 1.610 real sec...
[M::mem_process_seqs] Processed 699430 reads in 88.244 CPU sec, 2.781 real sec
sorting block 77, #reads = 1090709 mem = 536879072 peak = 1100951552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291067, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.62, 117.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704162 sequences (100000265 bp) in 2.413 CPU sec, 2.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292702, 11, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (239.03, 115.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (94, 107, 798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2206)
[M::mem_pestat] mean and std.dev: (288.00, 319.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2910)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 337, 1241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3429)
[M::mem_pestat] mean and std.dev: (546.40, 474.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4523)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 704854 sequences (100000261 bp) in 1.490 CPU sec, 1.495 real sec...
[M::mem_process_seqs] Processed 699940 reads in 149.914 CPU sec, 4.764 real sec
[M::mem_process_seqs] Processed 703852 reads in 99.379 CPU sec, 3.148 real sec
sorting block 78, #reads = 1089677 mem = 536878516 peak = 1100951552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292705, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (240.93, 117.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702144 sequences (100000212 bp) in 2.474 CPU sec, 2.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293632, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (236.71, 113.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703836 sequences (100000120 bp) in 1.520 CPU sec, 1.519 real sec...
[M::mem_process_seqs] Processed 704162 reads in 150.272 CPU sec, 4.769 real sec
[M::mem_process_seqs] Processed 704854 reads in 94.363 CPU sec, 2.992 real sec
sorting block 79, #reads = 1085662 mem = 536882311 peak = 1100951552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292277, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 419, 905)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2341)
[M::mem_pestat] mean and std.dev: (506.54, 331.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3059)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 220, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (246.93, 120.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 704120 sequences (100000165 bp) in 2.363 CPU sec, 2.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292812, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 724, 1721)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4815)
[M::mem_pestat] mean and std.dev: (888.40, 818.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6362)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (240.41, 117.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 703422 sequences (100000221 bp) in 1.460 CPU sec, 1.464 real sec...
[M::mem_process_seqs] Processed 702144 reads in 143.811 CPU sec, 4.569 real sec
sorting block 80, #reads = 1089042 mem = 536881415 peak = 1100951552
[M::mem_process_seqs] Processed 703836 reads in 90.190 CPU sec, 2.854 real sec
sorting block 81, #reads = 1090494 mem = 536880057 peak = 1114972160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293136, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (237.36, 114.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701784 sequences (100000166 bp) in 2.085 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292762, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.40, 113.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699592 sequences (100000052 bp) in 1.460 CPU sec, 1.459 real sec...
[M::mem_process_seqs] Processed 704120 reads in 143.344 CPU sec, 4.564 real sec
[M::mem_process_seqs] Processed 703422 reads in 91.085 CPU sec, 2.885 real sec
sorting block 82, #reads = 1092019 mem = 536879378 peak = 1114972160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 292692, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 318, 953)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2391)
[M::mem_pestat] mean and std.dev: (617.88, 574.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3110)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.98, 116.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701170 sequences (100000244 bp) in 2.307 CPU sec, 2.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291981, 8, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 209, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (249.73, 107.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.06, 119.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 272, 1943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5367)
[M::mem_pestat] mean and std.dev: (778.00, 825.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7079)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 700316 sequences (100000230 bp) in 1.430 CPU sec, 1.436 real sec...
[M::mem_process_seqs] Processed 701784 reads in 138.746 CPU sec, 4.406 real sec
[M::mem_process_seqs] Processed 699592 reads in 87.514 CPU sec, 2.773 real sec
sorting block 83, #reads = 1091982 mem = 536877397 peak = 1114972160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292412, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.13, 116.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699640 sequences (100000143 bp) in 2.452 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291859, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 200, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322)
[M::mem_pestat] mean and std.dev: (322.60, 306.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1721)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.27, 119.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701170 reads in 137.013 CPU sec, 4.355 real sec
[M::process] read 699318 sequences (100000077 bp) in 1.492 CPU sec, 1.502 real sec...
sorting block 84, #reads = 1091626 mem = 536878167 peak = 1114972160
[M::mem_process_seqs] Processed 700316 reads in 86.933 CPU sec, 2.746 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291450, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.58, 118.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 85, #reads = 1092662 mem = 536881995 peak = 1114972160
[M::process] read 698988 sequences (100000276 bp) in 2.352 CPU sec, 2.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290838, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.99, 119.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698648 sequences (100000022 bp) in 1.430 CPU sec, 1.436 real sec...
[M::mem_process_seqs] Processed 699640 reads in 136.391 CPU sec, 4.334 real sec
[M::mem_process_seqs] Processed 699318 reads in 86.191 CPU sec, 2.722 real sec
sorting block 86, #reads = 1091435 mem = 536883485 peak = 1114972160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291280, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.68, 119.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698534 sequences (100000229 bp) in 2.414 CPU sec, 2.532 real sec...
[M::process] read 248102 sequences (35423504 bp) in 0.700 CPU sec, 0.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290627, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 404, 839)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2109)
[M::mem_pestat] mean and std.dev: (605.36, 469.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.69, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698988 reads in 135.171 CPU sec, 4.316 real sec
sorting block 87, #reads = 1091273 mem = 536879273 peak = 1114972160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290781, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 240, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150)
[M::mem_pestat] mean and std.dev: (223.88, 117.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1488)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.84, 120.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698534 reads in 76.659 CPU sec, 2.661 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 103225, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (243.75, 116.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 248102 reads in 40.270 CPU sec, 1.322 real sec
[M::mem_process_seqs] Processed 698648 reads in 185.891 CPU sec, 6.230 real sec
sorting block 88, #reads = 1090506 mem = 536886102 peak = 1114972160
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 97372958
overall: 70013362176 mem 11302.969 user 385.245 sys 1228.893 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1228.933 sec; CPU: 11688.217 sec
sorting block 89, #reads = 648326 mem = 319225633 peak = 1114972160
uniq header count 1
merging 90 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5228392729
output reads: 97868505
bam_mem_sort: 90 calls 33.666 user 0.552 sys 34.451 real
bam_write: 90 calls 72.190 user 2.316 sys 74.952 real
execute: 1 calls 17.198 user 0.960 sys 1256.484 real
merge_files: 1 calls 17.173 user 0.935 sys 26.656 real
parse_chunk: 1434 calls 45.240 user 11.955 sys 58.315 real
read_chunk: 3872 calls 36.650 user 37.013 sys 340.247 real
sort_block: 1 calls 28.278 user 36.391 sys 328.235 real
write_chunk: 2436 calls 16.832 user 0.710 sys 25.831 real
overall: 2999951360 mem 1036.990 user 63.152 sys 1256.506 real
ls: cannot access '*.cram': No such file or directory