Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fa/cec2e4d07e2762a03b209987e04a68/1136_5KC-T1-TDNA-1_A23MHWWLT4_1/1136_5KC-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/86/93f4c5a301cc22adb3c182f56ca8c8/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fa/cec2e4d07e2762a03b209987e04a68/1136_5KC-T1-TDNA-1_A23MHWWLT4_1/1136_5KC-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/86/93f4c5a301cc22adb3c182f56ca8c8/.command.run ==> STAGING COMPLETE (7 inputs) This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_5KC-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2065.534 CPU sec, 92.005 real sec [M::process] read 692466 sequences (100000128 bp) in 1.568 CPU sec, 1.578 real sec... [M::process] read 690530 sequences (100000183 bp) in 1.891 CPU sec, 1.893 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 286804, 11, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 249, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2564) [M::mem_pestat] mean and std.dev: (439.94, 442.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 274, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (298.57, 150.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1151, 1272, 1611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (231, 2531) [M::mem_pestat] mean and std.dev: (1131.40, 501.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3136) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 289, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (298.63, 168.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689798 sequences (100000290 bp) in 1.735 CPU sec, 1.787 real sec... [M::process] read 689178 sequences (100000030 bp) in 1.619 CPU sec, 1.685 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286128, 14, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 284, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (261.63, 172.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (306.67, 153.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (391, 496, 2118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5572) [M::mem_pestat] mean and std.dev: (995.86, 993.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7299) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 264, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (253.24, 109.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692466 reads in 202.527 CPU sec, 6.514 real sec [M::mem_process_seqs] Processed 690530 reads in 120.477 CPU sec, 3.934 real sec sorting block 0, #reads = 1085713 mem = 536877943 peak = 581611520 [M::process] read 688744 sequences (100000086 bp) in 2.300 CPU sec, 2.399 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285792, 7, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 204, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (218.66, 88.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 287, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (309.28, 153.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 277, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (263.30, 72.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688448 sequences (100000020 bp) in 1.730 CPU sec, 1.724 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 285798, 7, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 294, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (259.33, 107.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 291, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (312.29, 154.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 232, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (246.55, 117.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689798 reads in 179.064 CPU sec, 5.709 real sec [M::mem_process_seqs] Processed 689178 reads in 119.153 CPU sec, 3.820 real sec sorting block 1, #reads = 1084893 mem = 536878888 peak = 710586368 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285175, 12, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 203, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (216.06, 91.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (313.95, 155.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 225, 2096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5838) [M::mem_pestat] mean and std.dev: (740.00, 806.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7709) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 269, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (299.67, 170.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688086 sequences (100000028 bp) in 2.328 CPU sec, 2.402 real sec... [M::process] read 687506 sequences (100000265 bp) in 1.679 CPU sec, 1.685 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285510, 11, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (236.83, 133.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (316.88, 156.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 440, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4183) [M::mem_pestat] mean and std.dev: (685.90, 585.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (222.44, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688744 reads in 173.948 CPU sec, 5.530 real sec [M::mem_process_seqs] Processed 688448 reads in 115.204 CPU sec, 3.678 real sec sorting block 2, #reads = 1085491 mem = 536878311 peak = 825970688 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 284942, 7, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 232, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2406) [M::mem_pestat] mean and std.dev: (551.19, 652.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (317.22, 157.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 346, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (277.57, 116.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687572 sequences (100000163 bp) in 2.711 CPU sec, 2.807 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285114, 5, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 197, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (251.15, 158.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (318.93, 157.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 217, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (235.27, 113.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687102 sequences (100000055 bp) in 2.066 CPU sec, 2.091 real sec... [M::mem_process_seqs] Processed 688086 reads in 177.389 CPU sec, 5.616 real sec sorting block 3, #reads = 1084353 mem = 536879305 peak = 825970688 [M::mem_process_seqs] Processed 687506 reads in 121.345 CPU sec, 3.860 real sec sorting block 4, #reads = 1083525 mem = 536881919 peak = 906772480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284654, 18, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 228, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (248.95, 113.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (319.92, 157.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (248, 612, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2882) [M::mem_pestat] mean and std.dev: (557.00, 328.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 274, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (273.26, 149.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686852 sequences (100000049 bp) in 2.249 CPU sec, 2.299 real sec... [M::process] read 687096 sequences (100000184 bp) in 2.014 CPU sec, 2.017 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284761, 11, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 273, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (280.79, 119.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 302, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (322.65, 159.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 45, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1389) [M::mem_pestat] mean and std.dev: (332.73, 385.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 270, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (275.52, 113.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687572 reads in 185.577 CPU sec, 5.938 real sec [M::mem_process_seqs] Processed 687102 reads in 124.462 CPU sec, 3.966 real sec sorting block 5, #reads = 1084033 mem = 536880791 peak = 906772480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284883, 10, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 354, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (265.93, 108.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (322.13, 158.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 476, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (372.89, 262.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1424) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 251, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (292.72, 143.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686772 sequences (100000055 bp) in 2.938 CPU sec, 3.055 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285561, 14, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 252, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2715) [M::mem_pestat] mean and std.dev: (418.17, 493.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (321.61, 158.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (567, 736, 1611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3699) [M::mem_pestat] mean and std.dev: (1041.50, 837.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (245.95, 95.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686538 sequences (100000135 bp) in 2.217 CPU sec, 2.224 real sec... [M::mem_process_seqs] Processed 686852 reads in 189.134 CPU sec, 6.018 real sec [M::mem_process_seqs] Processed 687096 reads in 126.292 CPU sec, 4.012 real sec sorting block 6, #reads = 1083606 mem = 536879653 peak = 906772480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285092, 10, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 236, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189) [M::mem_pestat] mean and std.dev: (305.00, 178.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 299, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (319.85, 156.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (469, 469, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (469, 469) [M::mem_pestat] mean and std.dev: (469.00, 0.00) [M::mem_pestat] low and high boundaries for proper pairs: (469, 469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 295, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (303.53, 149.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686768 sequences (100000257 bp) in 2.750 CPU sec, 2.883 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284579, 9, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 312, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (329.05, 161.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 299, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (319.60, 155.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 282, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (291.15, 123.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1363) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686638 sequences (100000280 bp) in 2.155 CPU sec, 2.159 real sec... [M::mem_process_seqs] Processed 686772 reads in 186.970 CPU sec, 5.961 real sec [M::mem_process_seqs] Processed 686538 reads in 125.716 CPU sec, 3.990 real sec sorting block 7, #reads = 1082977 mem = 536881676 peak = 906772480 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284933, 4, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 225, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (230.17, 101.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 297, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (316.47, 153.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 221, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 464) [M::mem_pestat] mean and std.dev: (229.76, 73.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687088 sequences (100000199 bp) in 2.846 CPU sec, 2.978 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285064, 8, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 199, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (249.72, 150.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 296, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (315.59, 152.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 237, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (236.89, 99.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687186 sequences (100000162 bp) in 2.181 CPU sec, 2.199 real sec... [M::mem_process_seqs] Processed 686768 reads in 185.585 CPU sec, 5.912 real sec sorting block 8, #reads = 1082878 mem = 536882290 peak = 906772480 [M::mem_process_seqs] Processed 686638 reads in 122.413 CPU sec, 3.884 real sec sorting block 9, #reads = 1084062 mem = 536877875 peak = 928268288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285229, 12, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 212, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683) [M::mem_pestat] mean and std.dev: (234.54, 103.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 295, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (313.89, 151.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (630, 910, 1540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3360) [M::mem_pestat] mean and std.dev: (846.55, 601.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 241, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (259.89, 114.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687000 sequences (100000275 bp) in 2.566 CPU sec, 2.699 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284982, 2, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 291, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (258.04, 121.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 294, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (313.59, 151.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 490, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (394.81, 219.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1626) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686742 sequences (100000036 bp) in 2.130 CPU sec, 2.121 real sec... [M::mem_process_seqs] Processed 687088 reads in 183.806 CPU sec, 5.862 real sec [M::mem_process_seqs] Processed 687186 reads in 123.118 CPU sec, 3.909 real sec sorting block 10, #reads = 1082620 mem = 536881802 peak = 928268288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285121, 3, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 248, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (247.32, 104.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 296, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (314.72, 152.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 255, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (255.33, 106.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686632 sequences (100000259 bp) in 2.987 CPU sec, 3.128 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285106, 14, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 195, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (249.36, 135.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 297, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (315.87, 152.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (926, 1054, 2908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6872) [M::mem_pestat] mean and std.dev: (1960.71, 1436.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 253, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (245.21, 101.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687000 reads in 183.393 CPU sec, 5.846 real sec [M::process] read 686376 sequences (100000091 bp) in 2.295 CPU sec, 2.325 real sec... [M::mem_process_seqs] Processed 686742 reads in 124.739 CPU sec, 3.953 real sec sorting block 11, #reads = 1083468 mem = 536877500 peak = 928268288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285029, 11, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 224, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (235.74, 124.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 299, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (317.30, 153.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (78, 605, 2925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8619) [M::mem_pestat] mean and std.dev: (774.56, 953.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11466) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 256, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (285.04, 136.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687916 sequences (100000113 bp) in 2.733 CPU sec, 2.874 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 284162, 5, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 227, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (291.39, 170.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 303, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (321.40, 155.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 226, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (244.37, 106.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686632 reads in 185.411 CPU sec, 5.922 real sec [M::process] read 690286 sequences (100000301 bp) in 2.367 CPU sec, 2.378 real sec... sorting block 12, #reads = 1081490 mem = 536878178 peak = 928268288 [M::mem_process_seqs] Processed 686376 reads in 124.465 CPU sec, 3.940 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284824, 11, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 201, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (252.43, 125.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 297, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (316.51, 154.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (965, 1859, 5184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13622) [M::mem_pestat] mean and std.dev: (2846.55, 2655.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17841) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 269, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (263.85, 122.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 13, #reads = 1079621 mem = 536878122 peak = 928268288 [M::process] read 690076 sequences (100000285 bp) in 3.098 CPU sec, 3.210 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285950, 4, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 192, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (201.55, 73.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 283, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (306.99, 153.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 293, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (284.57, 96.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687916 reads in 186.584 CPU sec, 5.942 real sec [M::process] read 689318 sequences (100000261 bp) in 2.176 CPU sec, 2.185 real sec... [M::mem_process_seqs] Processed 690286 reads in 122.837 CPU sec, 3.900 real sec sorting block 14, #reads = 1082570 mem = 536879929 peak = 928268288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 285849, 2, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 364, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272) [M::mem_pestat] mean and std.dev: (369.56, 251.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 287, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (309.17, 154.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 339, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965) [M::mem_pestat] mean and std.dev: (292.83, 151.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689210 sequences (100000014 bp) in 2.684 CPU sec, 2.796 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285693, 2, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 338, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311) [M::mem_pestat] mean and std.dev: (365.54, 183.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (312.32, 155.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 262, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840) [M::mem_pestat] mean and std.dev: (291.54, 136.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690076 reads in 174.742 CPU sec, 5.568 real sec [M::process] read 688930 sequences (100000136 bp) in 2.202 CPU sec, 2.213 real sec... [M::mem_process_seqs] Processed 689318 reads in 116.679 CPU sec, 3.694 real sec sorting block 15, #reads = 1086589 mem = 536879084 peak = 928268288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285762, 17, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 319, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (332.45, 164.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 292, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (312.60, 154.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (479, 1618, 1618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3896) [M::mem_pestat] mean and std.dev: (1152.31, 631.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5035) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 254, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (239.67, 107.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688310 sequences (100000036 bp) in 2.985 CPU sec, 3.081 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 285919, 9, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 221, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (230.41, 62.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (315.76, 156.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 247, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (249.74, 98.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689210 reads in 173.801 CPU sec, 5.516 real sec [M::process] read 688064 sequences (100000292 bp) in 1.990 CPU sec, 1.993 real sec... [M::mem_process_seqs] Processed 688930 reads in 116.529 CPU sec, 3.690 real sec sorting block 16, #reads = 1085112 mem = 536877913 peak = 928268288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 285770, 1, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 357, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (333.65, 169.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (317.49, 157.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 266, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1726) [M::mem_pestat] mean and std.dev: (368.91, 266.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2239) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687584 sequences (100000057 bp) in 2.674 CPU sec, 2.753 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285037, 7, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 264, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370) [M::mem_pestat] mean and std.dev: (346.29, 254.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (318.76, 157.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 366, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2127) [M::mem_pestat] mean and std.dev: (366.49, 216.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2750) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688310 reads in 168.192 CPU sec, 5.338 real sec [M::process] read 687460 sequences (100000221 bp) in 2.269 CPU sec, 2.283 real sec... sorting block 17, #reads = 1085998 mem = 536883261 peak = 928268288 [M::mem_process_seqs] Processed 688064 reads in 114.097 CPU sec, 3.608 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 284950, 9, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 217, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (285.94, 159.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (319.25, 156.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 262, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (333.02, 195.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 18, #reads = 1084212 mem = 536879867 peak = 928268288 [M::process] read 687058 sequences (100000153 bp) in 3.349 CPU sec, 3.516 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285527, 4, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 234, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (215.94, 84.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 302, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (321.68, 158.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 259, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (293.45, 133.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687584 reads in 177.505 CPU sec, 5.678 real sec [M::process] read 686658 sequences (100000038 bp) in 2.650 CPU sec, 2.741 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285261, 11, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 252, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (227.81, 101.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 304, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (323.73, 159.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1184, 2102, 2102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3938) [M::mem_pestat] mean and std.dev: (1464.27, 839.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 328, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 663) [M::mem_pestat] mean and std.dev: (315.09, 118.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687058 reads in 101.935 CPU sec, 3.233 real sec [M::process] read 686444 sequences (100000283 bp) in 1.961 CPU sec, 1.979 real sec... sorting block 19, #reads = 1085243 mem = 536877223 peak = 928268288 [M::mem_process_seqs] Processed 687460 reads in 257.390 CPU sec, 8.167 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 285149, 3, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 292, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1181) [M::mem_pestat] mean and std.dev: (334.47, 225.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 304, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (323.77, 158.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 298, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (272.50, 90.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 20, #reads = 1084396 mem = 536879115 peak = 928268288 [M::process] read 686728 sequences (100000253 bp) in 3.142 CPU sec, 3.260 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284485, 13, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 196, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (201.03, 77.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 306, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (324.82, 158.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (303, 352, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1830) [M::mem_pestat] mean and std.dev: (436.38, 262.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2339) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 280, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (319.47, 186.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686658 reads in 201.135 CPU sec, 6.371 real sec [M::mem_process_seqs] Processed 686444 reads in 124.110 CPU sec, 3.920 real sec [M::process] read 686568 sequences (100000158 bp) in 2.495 CPU sec, 2.590 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284880, 6, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 256, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267) [M::mem_pestat] mean and std.dev: (276.14, 147.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (320.56, 155.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994) [M::mem_pestat] mean and std.dev: (301.84, 152.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 21, #reads = 1083309 mem = 536880789 peak = 928268288 [M::process] read 686782 sequences (100000014 bp) in 3.151 CPU sec, 3.278 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 285035, 4, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 213, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (211.37, 72.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 299, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (317.92, 153.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 287, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1498) [M::mem_pestat] mean and std.dev: (431.74, 347.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686728 reads in 179.357 CPU sec, 5.715 real sec [M::mem_process_seqs] Processed 686568 reads in 122.581 CPU sec, 3.868 real sec [M::process] read 686588 sequences (100000136 bp) in 2.579 CPU sec, 2.626 real sec... sorting block 22, #reads = 1083056 mem = 536879830 peak = 928268288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284997, 13, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 182, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (204.17, 111.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 298, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (316.52, 152.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 581, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (420.50, 238.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 257, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (328.84, 232.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686962 sequences (100000172 bp) in 2.966 CPU sec, 3.090 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284757, 8, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 242, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108) [M::mem_pestat] mean and std.dev: (286.73, 169.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 299, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (316.63, 152.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 330, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (345.57, 225.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686782 reads in 175.823 CPU sec, 5.592 real sec [M::mem_process_seqs] Processed 686588 reads in 120.857 CPU sec, 3.802 real sec [M::process] read 686804 sequences (100000033 bp) in 2.470 CPU sec, 2.505 real sec... sorting block 23, #reads = 1081908 mem = 536880243 peak = 928268288 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285029, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 232, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (248.67, 116.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 297, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (315.88, 152.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 251, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (271.55, 123.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686976 sequences (100000293 bp) in 2.753 CPU sec, 2.872 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284425, 8, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 241, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (248.27, 140.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 299, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (317.47, 153.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 232, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (229.73, 88.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686962 reads in 176.373 CPU sec, 5.589 real sec [M::process] read 687246 sequences (100000281 bp) in 2.080 CPU sec, 2.077 real sec... sorting block 24, #reads = 1082251 mem = 536878910 peak = 928268288 [M::mem_process_seqs] Processed 686804 reads in 121.748 CPU sec, 3.848 real sec sorting block 25, #reads = 1082201 mem = 536878188 peak = 953679872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284756, 13, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 213, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (198.76, 72.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 296, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (315.52, 152.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (347, 702, 5213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14945) [M::mem_pestat] mean and std.dev: (1908.15, 2214.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 192, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (188.95, 76.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687112 sequences (100000200 bp) in 3.112 CPU sec, 3.214 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 285276, 10, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 215, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405) [M::mem_pestat] mean and std.dev: (278.49, 185.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 296, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (315.58, 153.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 726, 2630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7240) [M::mem_pestat] mean and std.dev: (1396.70, 1449.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 328, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (303.98, 117.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686976 reads in 179.028 CPU sec, 5.657 real sec [M::process] read 686832 sequences (100000079 bp) in 2.266 CPU sec, 2.266 real sec... [M::mem_process_seqs] Processed 687246 reads in 122.025 CPU sec, 3.847 real sec sorting block 26, #reads = 1082085 mem = 536878828 peak = 953679872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285316, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 289, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (451.79, 432.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (318.74, 155.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 335, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1275) [M::mem_pestat] mean and std.dev: (360.21, 194.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688164 sequences (100000297 bp) in 3.137 CPU sec, 3.263 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285180, 4, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 306, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (299.79, 136.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 301, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (320.62, 156.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 338, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (321.31, 137.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687112 reads in 180.343 CPU sec, 5.711 real sec [M::process] read 691158 sequences (100000152 bp) in 2.375 CPU sec, 2.380 real sec... [M::mem_process_seqs] Processed 686832 reads in 123.121 CPU sec, 3.885 real sec sorting block 27, #reads = 1082136 mem = 536878814 peak = 953679872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 285111, 0, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 277, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (330.28, 215.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (315.91, 155.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 293, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998) [M::mem_pestat] mean and std.dev: (291.25, 158.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691316 sequences (100000245 bp) in 2.857 CPU sec, 2.958 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 286102, 15, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 247, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (223.71, 75.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (303.34, 152.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (347, 714, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2690) [M::mem_pestat] mean and std.dev: (643.25, 368.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 259, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1267) [M::mem_pestat] mean and std.dev: (347.30, 223.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1627) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691004 sequences (100000212 bp) in 1.800 CPU sec, 1.802 real sec... [M::mem_process_seqs] Processed 688164 reads in 181.308 CPU sec, 5.758 real sec sorting block 28, #reads = 1080051 mem = 536876938 peak = 953679872 [M::mem_process_seqs] Processed 691158 reads in 120.103 CPU sec, 3.808 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 286662, 5, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 260, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (220.75, 100.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (304.35, 152.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 354, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (406.80, 225.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 29, #reads = 1084446 mem = 536879006 peak = 953679872 [M::process] read 690596 sequences (100000161 bp) in 2.604 CPU sec, 2.686 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 286743, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 207, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (193.61, 86.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 284, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (306.18, 152.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 317, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (308.09, 124.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689994 sequences (100000101 bp) in 1.999 CPU sec, 2.009 real sec... [M::mem_process_seqs] Processed 691316 reads in 174.137 CPU sec, 5.526 real sec [M::mem_process_seqs] Processed 691004 reads in 117.642 CPU sec, 3.728 real sec sorting block 30, #reads = 1086316 mem = 536877431 peak = 953679872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286591, 4, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 196, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (226.68, 123.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (307.43, 153.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 294, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (308.94, 118.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689366 sequences (100000066 bp) in 2.767 CPU sec, 2.851 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 286353, 5, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 326, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (316.91, 128.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 288, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (310.96, 155.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 280, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324) [M::mem_pestat] mean and std.dev: (356.91, 260.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689396 sequences (100000270 bp) in 1.970 CPU sec, 1.969 real sec... [M::mem_process_seqs] Processed 690596 reads in 173.742 CPU sec, 5.506 real sec [M::mem_process_seqs] Processed 689994 reads in 117.763 CPU sec, 3.742 real sec sorting block 31, #reads = 1087138 mem = 536878475 peak = 953679872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 286277, 11, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 212, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (237.90, 112.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (313.10, 155.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (508, 826, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2500) [M::mem_pestat] mean and std.dev: (789.91, 499.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 250, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (234.28, 99.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688686 sequences (100000105 bp) in 2.339 CPU sec, 2.425 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 286280, 9, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 204, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (295.17, 201.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 292, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (313.79, 156.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 235, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318) [M::mem_pestat] mean and std.dev: (301.39, 182.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687838 sequences (100000281 bp) in 1.970 CPU sec, 1.972 real sec... [M::mem_process_seqs] Processed 689366 reads in 175.441 CPU sec, 5.573 real sec [M::mem_process_seqs] Processed 689396 reads in 117.489 CPU sec, 3.725 real sec sorting block 32, #reads = 1084779 mem = 536877476 peak = 953679872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 286057, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 173, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (181.09, 63.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (316.61, 157.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 280, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (261.64, 103.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688024 sequences (100000135 bp) in 2.864 CPU sec, 2.976 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285144, 5, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 263, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916) [M::mem_pestat] mean and std.dev: (406.46, 394.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 298, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (319.39, 158.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 377, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (310.97, 126.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688686 reads in 176.406 CPU sec, 5.585 real sec [M::process] read 688098 sequences (100000166 bp) in 2.130 CPU sec, 2.135 real sec... sorting block 33, #reads = 1085329 mem = 536877870 peak = 953679872 [M::mem_process_seqs] Processed 687838 reads in 120.135 CPU sec, 3.796 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 285228, 12, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 207, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (197.33, 91.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 299, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (320.72, 159.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (4036, 7553, 7553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14587) [M::mem_pestat] mean and std.dev: (5619.00, 2668.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18104) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 346, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (365.20, 162.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1017) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 34, #reads = 1085209 mem = 536880659 peak = 953679872 [M::process] read 687600 sequences (100000122 bp) in 2.842 CPU sec, 2.901 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285518, 6, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 216, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (255.96, 111.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (320.46, 158.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 191, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (219.88, 85.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688024 reads in 175.313 CPU sec, 5.561 real sec [M::process] read 688092 sequences (100000235 bp) in 2.430 CPU sec, 2.455 real sec... [M::mem_process_seqs] Processed 688098 reads in 117.156 CPU sec, 3.686 real sec sorting block 35, #reads = 1084558 mem = 536880520 peak = 953679872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285290, 4, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 218, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1343) [M::mem_pestat] mean and std.dev: (276.00, 209.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (321.20, 158.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 187, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (77, 317) [M::mem_pestat] mean and std.dev: (180.54, 34.16) [M::mem_pestat] low and high boundaries for proper pairs: (29, 365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688126 sequences (100000056 bp) in 2.857 CPU sec, 2.994 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285824, 16, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 208, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (194.53, 101.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 291, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (312.46, 153.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1835, 1835, 3187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5891) [M::mem_pestat] mean and std.dev: (2147.94, 817.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 255, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (248.00, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687600 reads in 176.463 CPU sec, 5.610 real sec [M::process] read 687790 sequences (100000057 bp) in 2.330 CPU sec, 2.339 real sec... [M::mem_process_seqs] Processed 688092 reads in 120.690 CPU sec, 3.813 real sec sorting block 36, #reads = 1083374 mem = 536878935 peak = 953679872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 285355, 8, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 321, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (294.84, 101.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 291, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (312.69, 153.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (296.02, 156.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686714 sequences (100000210 bp) in 2.819 CPU sec, 2.892 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285556, 2, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 221, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (272.81, 174.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (317.48, 156.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 267, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (279.63, 122.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688126 reads in 177.169 CPU sec, 5.614 real sec [M::process] read 686790 sequences (100000170 bp) in 2.312 CPU sec, 2.321 real sec... [M::mem_process_seqs] Processed 687790 reads in 120.013 CPU sec, 3.785 real sec sorting block 37, #reads = 1082992 mem = 536879019 peak = 953679872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 284596, 12, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (252.96, 142.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 301, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (321.22, 156.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (208, 974, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2506) [M::mem_pestat] mean and std.dev: (585.00, 471.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3272) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 266, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (338.10, 182.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686820 sequences (100000246 bp) in 2.636 CPU sec, 2.738 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284994, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 335, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (357.20, 205.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (320.30, 156.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 259, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (257.08, 94.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686702 sequences (100000004 bp) in 2.229 CPU sec, 2.230 real sec... [M::mem_process_seqs] Processed 686714 reads in 175.919 CPU sec, 5.571 real sec sorting block 38, #reads = 1083937 mem = 536880223 peak = 953679872 [M::mem_process_seqs] Processed 686790 reads in 118.791 CPU sec, 3.752 real sec sorting block 39, #reads = 1082983 mem = 536877593 peak = 996798464 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 285056, 4, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 220, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (262.71, 152.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 301, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (321.39, 157.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 315, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (333.66, 219.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686726 sequences (100000166 bp) in 2.941 CPU sec, 3.034 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285071, 10, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 242, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1438) [M::mem_pestat] mean and std.dev: (252.00, 137.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (320.79, 157.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 188, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346) [M::mem_pestat] mean and std.dev: (261.67, 210.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 279, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (259.62, 79.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686820 reads in 177.809 CPU sec, 5.622 real sec [M::process] read 687640 sequences (100000225 bp) in 2.328 CPU sec, 2.324 real sec... [M::mem_process_seqs] Processed 686702 reads in 119.833 CPU sec, 3.782 real sec sorting block 40, #reads = 1081990 mem = 536878432 peak = 996798464 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284921, 5, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 220, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (216.81, 85.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (319.84, 155.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 317, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (276.71, 102.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686342 sequences (100000012 bp) in 2.676 CPU sec, 2.771 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285249, 7, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 274, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (277.80, 128.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (318.23, 156.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 275, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (283.17, 167.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689308 sequences (100000274 bp) in 2.220 CPU sec, 2.215 real sec... [M::mem_process_seqs] Processed 686726 reads in 178.626 CPU sec, 5.661 real sec [M::mem_process_seqs] Processed 687640 reads in 121.162 CPU sec, 3.825 real sec sorting block 41, #reads = 1080899 mem = 536884094 peak = 996798464 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284226, 3, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 240, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (297.08, 140.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 305, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (326.21, 160.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 275, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (306.10, 141.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1239) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690814 sequences (100000262 bp) in 2.711 CPU sec, 2.790 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285222, 8, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 215, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (282.77, 191.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (313.00, 156.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 272, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1129) [M::mem_pestat] mean and std.dev: (308.56, 174.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690386 sequences (100000272 bp) in 2.170 CPU sec, 2.169 real sec... [M::mem_process_seqs] Processed 686342 reads in 177.983 CPU sec, 5.634 real sec sorting block 42, #reads = 1081720 mem = 536875898 peak = 996798464 [M::mem_process_seqs] Processed 689308 reads in 121.125 CPU sec, 3.827 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285913, 13, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 217, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060) [M::mem_pestat] mean and std.dev: (295.46, 214.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (304.81, 153.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (998, 1753, 3108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7328) [M::mem_pestat] mean and std.dev: (1948.92, 1055.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 266, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (250.09, 97.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 43, #reads = 1081304 mem = 536879255 peak = 996798464 [M::process] read 690386 sequences (100000250 bp) in 2.768 CPU sec, 2.862 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286043, 3, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 307, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (295.18, 115.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 282, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (305.84, 152.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 296, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (330.07, 239.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690814 reads in 171.089 CPU sec, 5.412 real sec [M::process] read 690682 sequences (100000138 bp) in 2.325 CPU sec, 2.331 real sec... [M::mem_process_seqs] Processed 690386 reads in 113.388 CPU sec, 3.567 real sec sorting block 44, #reads = 1086919 mem = 536879430 peak = 996798464 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 285796, 14, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 250, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (234.76, 94.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (306.54, 152.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 677, 2667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7715) [M::mem_pestat] mean and std.dev: (1036.93, 1082.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 255, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (331.42, 231.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690250 sequences (100000064 bp) in 2.581 CPU sec, 2.691 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 286098, 13, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 185, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (207.97, 104.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 283, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (306.67, 154.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 513, 2481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7035) [M::mem_pestat] mean and std.dev: (1353.23, 1635.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 264, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (263.66, 93.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689712 sequences (100000140 bp) in 1.770 CPU sec, 1.770 real sec... [M::mem_process_seqs] Processed 690386 reads in 171.489 CPU sec, 5.463 real sec [M::mem_process_seqs] Processed 690682 reads in 115.620 CPU sec, 3.658 real sec sorting block 45, #reads = 1087587 mem = 536875657 peak = 996798464 [M::process] read 689658 sequences (100000107 bp) in 2.796 CPU sec, 2.890 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285934, 4, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 286, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (279.67, 124.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 288, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (310.78, 155.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 346, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (361.00, 181.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689712 reads in 96.551 CPU sec, 3.023 real sec [M::process] read 688870 sequences (100000035 bp) in 2.410 CPU sec, 2.428 real sec... sorting block 46, #reads = 1086292 mem = 536880460 peak = 996798464 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 286098, 7, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 296, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (225.76, 121.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 287, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (310.09, 155.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 391, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (396.88, 235.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285764, 20, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 319, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (303.06, 118.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 290, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (312.01, 155.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 2452, 2836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7278) [M::mem_pestat] mean and std.dev: (1607.11, 1074.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9499) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 419, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (382.92, 149.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688482 sequences (100000238 bp) in 1.754 CPU sec, 1.756 real sec... [M::mem_process_seqs] Processed 690250 reads in 268.455 CPU sec, 8.503 real sec [M::mem_process_seqs] Processed 689658 reads in 137.456 CPU sec, 4.374 real sec sorting block 47, #reads = 1085781 mem = 536876485 peak = 996798464 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285846, 12, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 263, 1478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4028) [M::mem_pestat] mean and std.dev: (710.65, 786.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 292, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (314.37, 156.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (405, 1138, 3060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8370) [M::mem_pestat] mean and std.dev: (1481.00, 1148.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11025) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 221, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (233.64, 108.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688684 sequences (100000050 bp) in 2.896 CPU sec, 3.014 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285631, 6, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 184, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (239.46, 152.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (316.82, 157.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 238, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (292.95, 168.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688870 reads in 171.594 CPU sec, 5.437 real sec [M::process] read 688176 sequences (100000010 bp) in 2.429 CPU sec, 2.460 real sec... sorting block 48, #reads = 1085485 mem = 536881035 peak = 996798464 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285177, 6, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 199, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (237.40, 105.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 297, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (318.76, 159.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 247, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (352.98, 229.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687770 sequences (100000091 bp) in 1.816 CPU sec, 1.817 real sec... [M::mem_process_seqs] Processed 688684 reads in 99.470 CPU sec, 3.160 real sec [M::mem_process_seqs] Processed 688482 reads in 251.989 CPU sec, 7.985 real sec sorting block 49, #reads = 1086057 mem = 536883618 peak = 996798464 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285986, 4, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 242, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (279.35, 132.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (319.12, 158.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 411, 928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2258) [M::mem_pestat] mean and std.dev: (583.23, 497.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687592 sequences (100000114 bp) in 2.690 CPU sec, 2.804 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285330, 3, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 305, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (280.59, 135.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (320.78, 159.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 293, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108) [M::mem_pestat] mean and std.dev: (311.88, 178.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688176 reads in 192.566 CPU sec, 6.112 real sec [M::mem_process_seqs] Processed 687770 reads in 116.067 CPU sec, 3.655 real sec [M::process] read 687400 sequences (100000026 bp) in 2.637 CPU sec, 2.695 real sec... sorting block 50, #reads = 1085189 mem = 536881350 peak = 996798464 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285241, 9, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 291, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (348.59, 186.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 300, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (319.91, 157.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 200, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (197.92, 57.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686638 sequences (100000226 bp) in 2.640 CPU sec, 2.744 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285087, 18, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 196, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (235.83, 122.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 300, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (319.69, 156.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1299, 1428, 1998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3396) [M::mem_pestat] mean and std.dev: (1327.75, 719.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 316, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179) [M::mem_pestat] mean and std.dev: (352.16, 211.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687592 reads in 174.541 CPU sec, 5.536 real sec [M::mem_process_seqs] Processed 687400 reads in 118.150 CPU sec, 3.715 real sec [M::process] read 686586 sequences (100000231 bp) in 2.428 CPU sec, 2.445 real sec... sorting block 51, #reads = 1084427 mem = 536876192 peak = 996798464 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284762, 15, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 217, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (244.69, 132.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (321.68, 156.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 915, 2823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8269) [M::mem_pestat] mean and std.dev: (1558.73, 1406.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10992) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 221, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2022) [M::mem_pestat] mean and std.dev: (396.48, 389.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2639) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686454 sequences (100000199 bp) in 2.734 CPU sec, 2.833 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284687, 11, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 227, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (322.59, 259.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 303, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (322.16, 156.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 771, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3391) [M::mem_pestat] mean and std.dev: (914.09, 823.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 239, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 463) [M::mem_pestat] mean and std.dev: (240.34, 66.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686028 sequences (100000018 bp) in 1.818 CPU sec, 1.823 real sec... [M::mem_process_seqs] Processed 686638 reads in 175.000 CPU sec, 5.560 real sec sorting block 52, #reads = 1082884 mem = 536878629 peak = 996798464 [M::mem_process_seqs] Processed 686586 reads in 118.602 CPU sec, 3.751 real sec sorting block 53, #reads = 1081695 mem = 536879246 peak = 1020137472 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284922, 5, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 269, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (266.61, 93.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 304, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (322.25, 155.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 323, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (410.91, 308.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686070 sequences (100000251 bp) in 2.699 CPU sec, 2.813 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285046, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 227, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (226.97, 93.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (199, 306, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (323.50, 156.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 254, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (245.64, 77.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686418 sequences (100000298 bp) in 2.210 CPU sec, 2.212 real sec... [M::mem_process_seqs] Processed 686454 reads in 177.318 CPU sec, 5.610 real sec [M::mem_process_seqs] Processed 686028 reads in 119.154 CPU sec, 3.758 real sec sorting block 54, #reads = 1082531 mem = 536877014 peak = 1020137472 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 284186, 6, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 201, 731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1939) [M::mem_pestat] mean and std.dev: (274.20, 263.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 304, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (322.15, 155.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 280, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (267.57, 104.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686330 sequences (100000105 bp) in 2.588 CPU sec, 2.692 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 284837, 8, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 207, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (212.86, 88.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 302, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (320.50, 154.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 224, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (237.57, 108.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686066 sequences (100000035 bp) in 2.251 CPU sec, 2.255 real sec... [M::mem_process_seqs] Processed 686070 reads in 175.850 CPU sec, 5.565 real sec [M::mem_process_seqs] Processed 686418 reads in 117.802 CPU sec, 3.718 real sec sorting block 55, #reads = 1081286 mem = 536876726 peak = 1020137472 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 284750, 10, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 183, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (195.62, 82.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 301, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (319.81, 154.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 313, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3587) [M::mem_pestat] mean and std.dev: (487.44, 624.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4766) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 260, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (345.07, 184.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690842 sequences (100000173 bp) in 3.027 CPU sec, 3.151 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 284639, 15, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 189, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (234.44, 138.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 302, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (320.20, 154.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 1006, 1711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4877) [M::mem_pestat] mean and std.dev: (880.67, 687.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6460) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 201, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (236.14, 105.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686330 reads in 175.089 CPU sec, 5.530 real sec [M::process] read 693996 sequences (100000141 bp) in 2.417 CPU sec, 2.427 real sec... sorting block 56, #reads = 1081579 mem = 536878592 peak = 1020137472 [M::mem_process_seqs] Processed 686066 reads in 117.792 CPU sec, 3.716 real sec sorting block 57, #reads = 1079625 mem = 536878156 peak = 1041059840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 286211, 4, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141) [M::mem_pestat] mean and std.dev: (290.18, 229.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (304.29, 152.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 236, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (287.00, 147.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693664 sequences (100000221 bp) in 2.837 CPU sec, 2.935 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 288062, 15, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 321, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116) [M::mem_pestat] mean and std.dev: (314.42, 166.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 266, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (294.66, 151.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (304, 472, 1356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3460) [M::mem_pestat] mean and std.dev: (498.92, 382.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 225, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (251.22, 116.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692672 sequences (100000016 bp) in 1.910 CPU sec, 1.907 real sec... [M::mem_process_seqs] Processed 690842 reads in 192.510 CPU sec, 6.110 real sec [M::mem_process_seqs] Processed 693996 reads in 128.077 CPU sec, 4.066 real sec sorting block 58, #reads = 1080851 mem = 536882158 peak = 1041059840 [M::process] read 690968 sequences (100000216 bp) in 2.948 CPU sec, 3.036 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 288144, 9, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 382, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2304) [M::mem_pestat] mean and std.dev: (457.61, 354.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 273, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (300.64, 153.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 244, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (268.60, 107.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690586 sequences (100000254 bp) in 2.290 CPU sec, 2.286 real sec... [M::mem_process_seqs] Processed 692672 reads in 104.133 CPU sec, 3.261 real sec sorting block 59, #reads = 1084681 mem = 536876304 peak = 1041059840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 287767, 2, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 217, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125) [M::mem_pestat] mean and std.dev: (285.35, 204.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (296.22, 152.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 251, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (241.88, 90.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 286861, 5, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 233, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (275.84, 114.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 282, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (307.45, 155.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 215, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (227.33, 116.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693664 reads in 292.983 CPU sec, 9.283 real sec [M::process] read 689604 sequences (100000158 bp) in 2.344 CPU sec, 2.360 real sec... [M::mem_process_seqs] Processed 690968 reads in 144.642 CPU sec, 4.602 real sec sorting block 60, #reads = 1084882 mem = 536884152 peak = 1041059840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286582, 8, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 273, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (292.98, 141.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (310.24, 157.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 295, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (306.70, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689480 sequences (100000255 bp) in 2.979 CPU sec, 3.106 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286069, 8, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 254, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (287.77, 117.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (314.02, 158.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 253, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2078) [M::mem_pestat] mean and std.dev: (483.64, 462.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690586 reads in 182.388 CPU sec, 5.777 real sec [M::process] read 689212 sequences (100000051 bp) in 2.079 CPU sec, 2.081 real sec... [M::mem_process_seqs] Processed 689604 reads in 122.195 CPU sec, 3.866 real sec sorting block 61, #reads = 1083960 mem = 536879970 peak = 1041059840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 286892, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 205, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (195.46, 77.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 292, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (316.50, 159.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 308, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1918) [M::mem_pestat] mean and std.dev: (404.16, 348.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688922 sequences (100000297 bp) in 3.067 CPU sec, 3.174 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286118, 8, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 323, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (288.50, 156.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 293, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (315.79, 158.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 343, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (306.20, 95.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689480 reads in 182.311 CPU sec, 5.763 real sec [M::process] read 689064 sequences (100000022 bp) in 2.320 CPU sec, 2.320 real sec... [M::mem_process_seqs] Processed 689212 reads in 122.562 CPU sec, 3.875 real sec sorting block 62, #reads = 1083297 mem = 536878267 peak = 1041059840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 285957, 3, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 199, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (303.02, 222.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 296, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (319.91, 161.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 317, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1651) [M::mem_pestat] mean and std.dev: (382.07, 232.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688034 sequences (100000260 bp) in 2.843 CPU sec, 2.922 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285841, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 189, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (247.86, 126.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 295, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (319.34, 161.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 251, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (238.88, 102.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687930 sequences (100000089 bp) in 2.288 CPU sec, 2.292 real sec... [M::mem_process_seqs] Processed 688922 reads in 186.485 CPU sec, 5.901 real sec sorting block 63, #reads = 1081743 mem = 536879263 peak = 1041059840 [M::mem_process_seqs] Processed 689064 reads in 125.356 CPU sec, 3.956 real sec sorting block 64, #reads = 1081986 mem = 536878303 peak = 1041059840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 285727, 18, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 230, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (242.72, 160.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (318.13, 158.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (527, 971, 2028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5030) [M::mem_pestat] mean and std.dev: (1343.39, 983.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 240, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (255.52, 85.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687496 sequences (100000230 bp) in 3.046 CPU sec, 3.146 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285826, 0, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 228, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776) [M::mem_pestat] mean and std.dev: (271.77, 160.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (318.81, 158.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 380, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (353.53, 114.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687332 sequences (100000249 bp) in 2.424 CPU sec, 2.424 real sec... [M::mem_process_seqs] Processed 688034 reads in 194.890 CPU sec, 6.171 real sec [M::mem_process_seqs] Processed 687930 reads in 132.839 CPU sec, 4.213 real sec sorting block 65, #reads = 1081334 mem = 536880561 peak = 1041059840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285426, 14, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 241, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (207.19, 76.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 297, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (320.25, 158.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 1660, 2770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7562) [M::mem_pestat] mean and std.dev: (1777.71, 1713.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9958) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 352, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (302.52, 114.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687746 sequences (100000102 bp) in 2.675 CPU sec, 2.793 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 285438, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 198, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (202.20, 90.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (319.88, 157.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 250, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (60, 450) [M::mem_pestat] mean and std.dev: (238.72, 59.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687542 sequences (100000004 bp) in 2.350 CPU sec, 2.347 real sec... [M::mem_process_seqs] Processed 687496 reads in 191.990 CPU sec, 6.084 real sec [M::mem_process_seqs] Processed 687332 reads in 126.955 CPU sec, 4.022 real sec sorting block 66, #reads = 1079955 mem = 536877682 peak = 1041059840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285263, 12, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 297, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (284.32, 133.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1242) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (318.54, 157.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 762, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2343) [M::mem_pestat] mean and std.dev: (765.00, 439.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2916) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 279, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (258.07, 117.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688226 sequences (100000040 bp) in 2.853 CPU sec, 2.954 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285094, 4, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 282, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002) [M::mem_pestat] mean and std.dev: (294.34, 185.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (317.76, 157.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 261, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (251.09, 100.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687882 sequences (100000113 bp) in 2.357 CPU sec, 2.358 real sec... [M::mem_process_seqs] Processed 687746 reads in 196.736 CPU sec, 6.236 real sec sorting block 67, #reads = 1081086 mem = 536881362 peak = 1041059840 [M::mem_process_seqs] Processed 687542 reads in 131.846 CPU sec, 4.177 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 286003, 6, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 253, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (268.25, 136.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 292, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (314.92, 156.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 264, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (258.51, 133.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687510 sequences (100000089 bp) in 2.955 CPU sec, 3.031 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285777, 8, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 263, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (351.44, 263.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 294, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (317.30, 157.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 294, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (297.96, 169.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688226 reads in 184.118 CPU sec, 5.828 real sec sorting block 68, #reads = 1079877 mem = 536877512 peak = 1041059840 [M::process] read 687770 sequences (100000224 bp) in 2.344 CPU sec, 2.349 real sec... [M::mem_process_seqs] Processed 687882 reads in 123.590 CPU sec, 3.901 real sec sorting block 69, #reads = 1079617 mem = 536881260 peak = 1047834624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 285498, 8, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 194, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (245.46, 135.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (317.22, 156.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 234, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (279.38, 184.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688676 sequences (100000201 bp) in 2.759 CPU sec, 2.835 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285259, 10, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 316, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1919) [M::mem_pestat] mean and std.dev: (436.35, 475.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (317.41, 157.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 966, 1560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3970) [M::mem_pestat] mean and std.dev: (882.56, 623.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5175) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 238, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (241.78, 141.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697006 sequences (100000059 bp) in 2.370 CPU sec, 2.378 real sec... [M::mem_process_seqs] Processed 687510 reads in 186.133 CPU sec, 5.897 real sec [M::mem_process_seqs] Processed 687770 reads in 126.141 CPU sec, 3.985 real sec sorting block 70, #reads = 1079701 mem = 536880301 peak = 1047834624 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 286240, 4, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 224, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (218.48, 75.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 291, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (314.68, 157.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 324, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (361.17, 199.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1372) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695278 sequences (100000260 bp) in 2.628 CPU sec, 2.719 real sec... [M::process] read 695334 sequences (100000121 bp) in 1.919 CPU sec, 1.919 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 288821, 7, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 195, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (199.24, 77.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (280.85, 142.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 232, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (269.83, 160.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688676 reads in 201.549 CPU sec, 6.417 real sec sorting block 71, #reads = 1078756 mem = 536880404 peak = 1047834624 [M::mem_process_seqs] Processed 697006 reads in 138.809 CPU sec, 4.417 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 288814, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 295, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (275.84, 169.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (288.30, 145.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 296, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (275.50, 105.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693748 sequences (100000201 bp) in 3.085 CPU sec, 3.172 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 288272, 5, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 185, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (211.47, 115.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 262, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (287.63, 144.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 241, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (238.91, 100.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693396 sequences (100000268 bp) in 2.430 CPU sec, 2.436 real sec... [M::mem_process_seqs] Processed 695278 reads in 204.203 CPU sec, 6.467 real sec sorting block 72, #reads = 1080246 mem = 536880185 peak = 1047834624 [M::mem_process_seqs] Processed 695334 reads in 139.865 CPU sec, 4.438 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 288426, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 228, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068) [M::mem_pestat] mean and std.dev: (264.76, 172.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (292.59, 145.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 273, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155) [M::mem_pestat] mean and std.dev: (318.08, 210.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 73, #reads = 1080101 mem = 536883238 peak = 1053499392 [M::process] read 692266 sequences (100000188 bp) in 2.730 CPU sec, 2.834 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 287646, 3, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 227, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087) [M::mem_pestat] mean and std.dev: (269.58, 123.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (295.57, 148.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 262, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (237.87, 87.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691144 sequences (100000195 bp) in 2.417 CPU sec, 2.431 real sec... [M::mem_process_seqs] Processed 693748 reads in 208.158 CPU sec, 6.637 real sec [M::mem_process_seqs] Processed 693396 reads in 141.997 CPU sec, 4.509 real sec sorting block 74, #reads = 1078760 mem = 536878026 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 287717, 16, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 248, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257) [M::mem_pestat] mean and std.dev: (262.77, 184.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 276, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (299.93, 149.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 860, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5719) [M::mem_pestat] mean and std.dev: (1090.69, 949.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 227, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1368) [M::mem_pestat] mean and std.dev: (294.58, 172.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690566 sequences (100000119 bp) in 3.130 CPU sec, 3.256 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286894, 11, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 268, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001) [M::mem_pestat] mean and std.dev: (252.83, 114.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 280, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (303.46, 150.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 264, 1679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4533) [M::mem_pestat] mean and std.dev: (653.82, 643.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 309, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (307.66, 150.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690312 sequences (100000135 bp) in 1.999 CPU sec, 2.012 real sec... [M::mem_process_seqs] Processed 692266 reads in 198.400 CPU sec, 6.318 real sec [M::mem_process_seqs] Processed 691144 reads in 134.619 CPU sec, 4.273 real sec sorting block 75, #reads = 1079612 mem = 536879797 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 286700, 4, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 295, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (302.10, 162.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 284, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (306.64, 152.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 311, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (295.94, 135.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690172 sequences (100000217 bp) in 3.189 CPU sec, 3.315 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286606, 4, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 211, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (291.33, 233.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (308.42, 154.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 238, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (245.87, 114.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690566 reads in 196.116 CPU sec, 6.249 real sec [M::process] read 689478 sequences (100000067 bp) in 2.495 CPU sec, 2.524 real sec... [M::mem_process_seqs] Processed 690312 reads in 132.059 CPU sec, 4.172 real sec sorting block 76, #reads = 1080778 mem = 536877928 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 286455, 4, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 309, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (378.50, 278.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (309.59, 155.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 281, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (236.22, 106.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 77, #reads = 1080842 mem = 536881992 peak = 1053499392 [M::process] read 689068 sequences (100000067 bp) in 3.154 CPU sec, 3.270 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286183, 12, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 274, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009) [M::mem_pestat] mean and std.dev: (252.12, 138.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 287, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (310.10, 154.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (600, 1462, 4154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11262) [M::mem_pestat] mean and std.dev: (2026.08, 1923.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 267, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (281.86, 103.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690172 reads in 194.057 CPU sec, 6.161 real sec [M::process] read 688402 sequences (100000216 bp) in 2.482 CPU sec, 2.492 real sec... [M::mem_process_seqs] Processed 689478 reads in 132.484 CPU sec, 4.179 real sec sorting block 78, #reads = 1080891 mem = 536877931 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286473, 13, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 225, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (218.62, 85.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (313.45, 155.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 965, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5081) [M::mem_pestat] mean and std.dev: (861.69, 695.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 279, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (265.98, 115.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688096 sequences (100000051 bp) in 2.939 CPU sec, 3.060 real sec... [M::process] read 687776 sequences (100000056 bp) in 1.760 CPU sec, 1.756 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285905, 7, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 341, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (331.03, 183.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (316.83, 158.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 234, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (236.97, 146.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689068 reads in 193.303 CPU sec, 6.145 real sec [M::mem_process_seqs] Processed 688402 reads in 130.165 CPU sec, 4.139 real sec sorting block 79, #reads = 1080381 mem = 536881747 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 286064, 13, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 325, 1625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4465) [M::mem_pestat] mean and std.dev: (903.55, 970.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (316.95, 157.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 489, 1926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5662) [M::mem_pestat] mean and std.dev: (1011.69, 1363.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 292, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3194) [M::mem_pestat] mean and std.dev: (522.20, 438.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688138 sequences (100000173 bp) in 3.143 CPU sec, 3.240 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285487, 13, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 363, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1389) [M::mem_pestat] mean and std.dev: (416.43, 273.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (320.10, 160.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 700, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3192) [M::mem_pestat] mean and std.dev: (642.00, 470.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 265, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (302.33, 134.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687568 sequences (100000145 bp) in 2.270 CPU sec, 2.267 real sec... [M::mem_process_seqs] Processed 688096 reads in 189.786 CPU sec, 6.003 real sec sorting block 80, #reads = 1079781 mem = 536879566 peak = 1053499392 [M::mem_process_seqs] Processed 687776 reads in 127.808 CPU sec, 4.035 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285839, 6, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 299, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220) [M::mem_pestat] mean and std.dev: (333.38, 255.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 294, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (318.22, 159.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 343, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (328.66, 117.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688366 sequences (100000101 bp) in 2.903 CPU sec, 2.987 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285577, 13, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 197, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (196.93, 82.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (320.97, 160.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (491, 686, 3909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10745) [M::mem_pestat] mean and std.dev: (1618.92, 1793.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14163) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 204, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (252.92, 127.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687940 sequences (100000013 bp) in 2.310 CPU sec, 2.303 real sec... [M::mem_process_seqs] Processed 688138 reads in 186.085 CPU sec, 5.899 real sec sorting block 81, #reads = 1080479 mem = 536880296 peak = 1053499392 [M::mem_process_seqs] Processed 687568 reads in 125.739 CPU sec, 3.989 real sec sorting block 82, #reads = 1080799 mem = 536879742 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 285933, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 291, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (283.61, 117.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (318.34, 160.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 235, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (285.50, 189.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688202 sequences (100000100 bp) in 3.087 CPU sec, 3.156 real sec... [M::process] read 694170 sequences (100000078 bp) in 1.859 CPU sec, 1.862 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285641, 9, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (304.19, 154.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (322.27, 163.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 289, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (369.47, 235.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688366 reads in 195.488 CPU sec, 6.205 real sec [M::mem_process_seqs] Processed 687940 reads in 131.023 CPU sec, 4.166 real sec sorting block 83, #reads = 1078870 mem = 536882135 peak = 1053499392 [M::process] read 694348 sequences (100000098 bp) in 3.079 CPU sec, 3.163 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 287293, 8, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 230, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (244.07, 120.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (293.43, 149.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 315, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (335.98, 151.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693264 sequences (100000105 bp) in 2.429 CPU sec, 2.433 real sec... [M::mem_process_seqs] Processed 694170 reads in 111.373 CPU sec, 3.508 real sec sorting block 84, #reads = 1080709 mem = 536882301 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285325, 14, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 363, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (312.80, 210.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (320.53, 162.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1142) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 346, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (478.43, 282.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 359, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268) [M::mem_pestat] mean and std.dev: (364.03, 237.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 288328, 6, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 218, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016) [M::mem_pestat] mean and std.dev: (350.88, 299.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 266, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (293.73, 150.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1049) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 343, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163) [M::mem_pestat] mean and std.dev: (337.97, 163.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692064 sequences (100000098 bp) in 2.163 CPU sec, 2.169 real sec... [M::mem_process_seqs] Processed 688202 reads in 321.520 CPU sec, 10.203 real sec [M::mem_process_seqs] Processed 694348 reads in 157.696 CPU sec, 5.044 real sec sorting block 85, #reads = 1076130 mem = 536878266 peak = 1053499392 [M::process] read 691670 sequences (100000097 bp) in 3.349 CPU sec, 3.532 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 286879, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 258, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (260.03, 129.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 276, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (303.02, 154.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 264, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (281.83, 110.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692064 reads in 100.123 CPU sec, 3.148 real sec [M::process] read 690360 sequences (100000008 bp) in 2.438 CPU sec, 2.455 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 287908, 4, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 216, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420) [M::mem_pestat] mean and std.dev: (337.35, 232.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (296.97, 151.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 252, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (261.38, 117.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 86, #reads = 1084955 mem = 536878596 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 287194, 18, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 236, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (231.10, 137.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 278, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (303.53, 153.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (6, 834, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3078) [M::mem_pestat] mean and std.dev: (754.89, 702.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 265, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (272.23, 112.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693264 reads in 280.084 CPU sec, 8.867 real sec [M::process] read 690404 sequences (100000183 bp) in 2.062 CPU sec, 2.073 real sec... [M::mem_process_seqs] Processed 691670 reads in 141.577 CPU sec, 4.519 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286348, 15, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 340, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2126) [M::mem_pestat] mean and std.dev: (368.32, 391.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (307.97, 154.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1119, 2278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5618) [M::mem_pestat] mean and std.dev: (1474.13, 1304.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 309, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (316.35, 133.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 87, #reads = 1084477 mem = 536878856 peak = 1053499392 [M::process] read 690112 sequences (100000281 bp) in 3.254 CPU sec, 3.446 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 286453, 1, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 281, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (286.75, 148.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 282, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (306.56, 154.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 323, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (326.51, 185.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690360 reads in 182.052 CPU sec, 5.831 real sec [M::process] read 689840 sequences (100000106 bp) in 2.417 CPU sec, 2.471 real sec... [M::mem_process_seqs] Processed 690404 reads in 124.155 CPU sec, 3.957 real sec sorting block 88, #reads = 1084635 mem = 536876503 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 286172, 6, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 208, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919) [M::mem_pestat] mean and std.dev: (224.56, 132.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 285, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (308.12, 154.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 286, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (296.96, 165.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689622 sequences (100000055 bp) in 2.863 CPU sec, 3.057 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286304, 4, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 168, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (223.10, 120.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 287, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (309.07, 153.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 209, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (192.75, 47.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690112 reads in 185.296 CPU sec, 5.962 real sec [M::mem_process_seqs] Processed 689840 reads in 125.462 CPU sec, 3.980 real sec [M::process] read 689918 sequences (100000013 bp) in 2.526 CPU sec, 2.626 real sec... sorting block 89, #reads = 1084609 mem = 536879778 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286079, 8, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 246, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (269.07, 112.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 286, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (309.25, 153.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 233, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (241.93, 105.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690116 sequences (100000077 bp) in 3.188 CPU sec, 3.675 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 286181, 4, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 223, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (247.53, 134.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (309.60, 154.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 309, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (285.83, 150.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689622 reads in 189.988 CPU sec, 6.181 real sec [M::mem_process_seqs] Processed 689918 reads in 128.736 CPU sec, 4.136 real sec sorting block 90, #reads = 1083751 mem = 536885530 peak = 1053499392 [M::process] read 690632 sequences (100000007 bp) in 2.854 CPU sec, 3.025 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 286443, 9, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 278, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (354.46, 281.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 285, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (308.88, 154.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 224, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (276.26, 149.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 91, #reads = 1083580 mem = 536879761 peak = 1053499392 [M::process] read 690866 sequences (100000075 bp) in 2.889 CPU sec, 3.155 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 286530, 3, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 255, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (259.96, 114.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (303.15, 151.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 334, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (274.61, 113.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690116 reads in 196.667 CPU sec, 6.336 real sec [M::mem_process_seqs] Processed 690632 reads in 132.495 CPU sec, 4.215 real sec [M::process] read 690554 sequences (100000142 bp) in 2.853 CPU sec, 2.930 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 286612, 8, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 295, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2163) [M::mem_pestat] mean and std.dev: (312.20, 268.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (304.20, 152.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 307, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034) [M::mem_pestat] mean and std.dev: (308.67, 171.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 92, #reads = 1083343 mem = 536878091 peak = 1053499392 [M::process] read 689452 sequences (100000300 bp) in 3.235 CPU sec, 3.464 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 286731, 9, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 236, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (267.40, 132.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (309.82, 156.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 289, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (344.43, 222.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690866 reads in 195.204 CPU sec, 6.283 real sec [M::process] read 689036 sequences (100000129 bp) in 2.780 CPU sec, 2.948 real sec... sorting block 93, #reads = 1083145 mem = 536877969 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 286353, 3, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 244, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (273.06, 162.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 290, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (314.63, 159.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 263, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (272.80, 130.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689452 reads in 108.972 CPU sec, 3.479 real sec [M::process] read 688916 sequences (100000174 bp) in 2.493 CPU sec, 2.527 real sec... [M::mem_process_seqs] Processed 690554 reads in 293.232 CPU sec, 9.568 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 286233, 15, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 234, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (245.52, 116.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (315.51, 158.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (757, 1439, 5766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15784) [M::mem_pestat] mean and std.dev: (2332.73, 2131.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20793) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 222, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (258.93, 144.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 94, #reads = 1082865 mem = 536877556 peak = 1053499392 [M::process] read 688238 sequences (100000058 bp) in 3.419 CPU sec, 3.647 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 285806, 14, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 266, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238) [M::mem_pestat] mean and std.dev: (313.03, 217.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 293, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (317.24, 160.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1385, 2272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5578) [M::mem_pestat] mean and std.dev: (1374.21, 900.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7231) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 259, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (265.60, 122.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689036 reads in 217.824 CPU sec, 6.985 real sec [M::mem_process_seqs] Processed 688916 reads in 134.328 CPU sec, 4.270 real sec [M::process] read 689986 sequences (100000160 bp) in 2.822 CPU sec, 2.909 real sec... sorting block 95, #reads = 1081163 mem = 536880525 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285873, 8, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 204, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (236.03, 107.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (319.19, 160.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 254, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (268.07, 97.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688870 sequences (100000234 bp) in 2.963 CPU sec, 3.141 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 286360, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 290, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (289.91, 141.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (311.49, 158.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 202, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (227.48, 124.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688238 reads in 195.323 CPU sec, 6.237 real sec [M::process] read 694950 sequences (100000029 bp) in 2.267 CPU sec, 2.288 real sec... [M::mem_process_seqs] Processed 689986 reads in 135.641 CPU sec, 4.299 real sec sorting block 96, #reads = 1081659 mem = 536877244 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285774, 14, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2828) [M::mem_pestat] mean and std.dev: (619.36, 672.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (316.96, 161.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (557, 579, 3357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8957) [M::mem_pestat] mean and std.dev: (1877.00, 2091.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11757) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 237, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (252.61, 103.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696736 sequences (100000168 bp) in 3.363 CPU sec, 3.657 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 287800, 8, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 244, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (256.89, 111.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (290.71, 148.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 362, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185) [M::mem_pestat] mean and std.dev: (274.84, 143.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697226 sequences (100000231 bp) in 2.421 CPU sec, 2.431 real sec... [M::mem_process_seqs] Processed 688870 reads in 221.548 CPU sec, 7.145 real sec sorting block 97, #reads = 1080006 mem = 536885081 peak = 1053499392 [M::mem_process_seqs] Processed 694950 reads in 155.459 CPU sec, 4.978 real sec sorting block 98, #reads = 1073391 mem = 536880964 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 288900, 11, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 238, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800) [M::mem_pestat] mean and std.dev: (247.50, 94.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (283.16, 143.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 608, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3371) [M::mem_pestat] mean and std.dev: (830.45, 772.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 296, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (305.33, 145.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696160 sequences (100000126 bp) in 3.101 CPU sec, 3.355 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 289433, 7, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 261, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (234.59, 101.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 256, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (281.50, 142.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 995) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 205, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (219.55, 111.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695498 sequences (100000021 bp) in 2.475 CPU sec, 2.483 real sec... [M::mem_process_seqs] Processed 696736 reads in 214.216 CPU sec, 6.830 real sec [M::mem_process_seqs] Processed 697226 reads in 145.974 CPU sec, 4.632 real sec sorting block 99, #reads = 1081891 mem = 536877707 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 289327, 6, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 266, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (254.67, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (284.17, 143.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1005) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 236, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (303.60, 162.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693992 sequences (100000285 bp) in 3.331 CPU sec, 3.660 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 288609, 10, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 253, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (214.43, 78.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (171, 262, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (286.47, 143.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 371, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300) [M::mem_pestat] mean and std.dev: (341.70, 229.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 228, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (30, 425) [M::mem_pestat] mean and std.dev: (213.03, 56.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692368 sequences (100000136 bp) in 2.340 CPU sec, 2.343 real sec... [M::mem_process_seqs] Processed 696160 reads in 211.194 CPU sec, 6.766 real sec [M::mem_process_seqs] Processed 695498 reads in 145.242 CPU sec, 4.636 real sec sorting block 100, #reads = 1081813 mem = 536879056 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 288023, 1, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 170, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (256.48, 136.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (292.56, 146.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1035) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 255, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1368) [M::mem_pestat] mean and std.dev: (307.74, 208.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691558 sequences (100000211 bp) in 3.579 CPU sec, 3.866 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 287750, 14, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 233, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (284.26, 189.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (299.63, 150.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (458, 1371, 2755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7349) [M::mem_pestat] mean and std.dev: (2090.43, 2324.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11390) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 263, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (253.08, 99.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693992 reads in 206.941 CPU sec, 6.613 real sec [M::process] read 691996 sequences (100000242 bp) in 2.453 CPU sec, 2.499 real sec... sorting block 101, #reads = 1079944 mem = 536878526 peak = 1053499392 [M::mem_process_seqs] Processed 692368 reads in 139.227 CPU sec, 4.434 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 287293, 6, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 229, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189) [M::mem_pestat] mean and std.dev: (270.15, 157.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 278, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (302.10, 150.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 258, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (252.42, 99.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 102, #reads = 1081023 mem = 536879970 peak = 1053499392 [M::process] read 691492 sequences (100000122 bp) in 3.311 CPU sec, 3.431 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 286910, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 228, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (221.24, 108.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 279, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (303.27, 152.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 273, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (263.97, 112.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691558 reads in 189.214 CPU sec, 6.002 real sec [M::process] read 690978 sequences (100000147 bp) in 2.531 CPU sec, 2.554 real sec... [M::mem_process_seqs] Processed 691996 reads in 131.318 CPU sec, 4.143 real sec sorting block 103, #reads = 1082412 mem = 536880031 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 287287, 4, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 224, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (214.66, 91.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 281, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (305.35, 153.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 223, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (249.46, 122.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690710 sequences (100000076 bp) in 3.013 CPU sec, 3.109 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 286734, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 294, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (304.71, 182.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 282, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (307.98, 155.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 284, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (322.26, 176.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691492 reads in 192.619 CPU sec, 6.111 real sec [M::process] read 690294 sequences (100000172 bp) in 2.348 CPU sec, 2.357 real sec... [M::mem_process_seqs] Processed 690978 reads in 131.085 CPU sec, 4.148 real sec sorting block 104, #reads = 1082416 mem = 536882855 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 286576, 10, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 221, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (245.70, 115.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (309.07, 155.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 1013, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5123) [M::mem_pestat] mean and std.dev: (998.40, 936.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6801) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 269, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (254.14, 90.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689800 sequences (100000053 bp) in 2.914 CPU sec, 3.023 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 286481, 5, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 295, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1958) [M::mem_pestat] mean and std.dev: (425.00, 339.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (311.42, 156.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 254, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722) [M::mem_pestat] mean and std.dev: (254.72, 116.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689124 sequences (100000175 bp) in 2.049 CPU sec, 2.053 real sec... [M::mem_process_seqs] Processed 690710 reads in 189.291 CPU sec, 6.015 real sec [M::mem_process_seqs] Processed 690294 reads in 127.012 CPU sec, 4.034 real sec sorting block 105, #reads = 1082313 mem = 536879449 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 286445, 9, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 227, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (209.00, 80.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (311.10, 154.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 342, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002) [M::mem_pestat] mean and std.dev: (355.80, 205.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688474 sequences (100000069 bp) in 3.130 CPU sec, 3.280 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285718, 14, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 199, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940) [M::mem_pestat] mean and std.dev: (305.42, 246.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 292, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (314.32, 156.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (527, 539, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2708) [M::mem_pestat] mean and std.dev: (547.82, 264.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 264, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (256.86, 77.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689800 reads in 184.857 CPU sec, 5.861 real sec sorting block 106, #reads = 1082404 mem = 536880972 peak = 1053499392 [M::process] read 688002 sequences (100000105 bp) in 2.384 CPU sec, 2.397 real sec... [M::mem_process_seqs] Processed 689124 reads in 125.696 CPU sec, 3.958 real sec sorting block 107, #reads = 1081657 mem = 536878556 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 286003, 6, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 202, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354) [M::mem_pestat] mean and std.dev: (279.70, 250.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 295, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (317.18, 157.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 328, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (313.33, 120.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688122 sequences (100000206 bp) in 2.969 CPU sec, 3.078 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286182, 9, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 271, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (280.51, 147.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (319.99, 158.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 197, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (211.84, 104.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688474 reads in 179.183 CPU sec, 5.665 real sec [M::process] read 688196 sequences (100000069 bp) in 2.356 CPU sec, 2.365 real sec... [M::mem_process_seqs] Processed 688002 reads in 121.242 CPU sec, 3.814 real sec sorting block 108, #reads = 1081657 mem = 536882530 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 285497, 9, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (111, 157, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (209.29, 141.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (317.69, 157.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 223, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (219.64, 85.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687324 sequences (100000231 bp) in 2.905 CPU sec, 2.975 real sec... [M::process] read 287388 sequences (41691121 bp) in 1.110 CPU sec, 1.115 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 285096, 10, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 253, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (243.00, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (316.56, 156.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 236, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1584) [M::mem_pestat] mean and std.dev: (380.60, 484.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 286, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (293.53, 111.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688122 reads in 173.939 CPU sec, 5.528 real sec [M::mem_process_seqs] Processed 688196 reads in 118.387 CPU sec, 3.763 real sec sorting block 109, #reads = 1082492 mem = 536878431 peak = 1053499392 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 285763, 10, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 294, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208) [M::mem_pestat] mean and std.dev: (338.00, 200.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (318.33, 156.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 371, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2276) [M::mem_pestat] mean and std.dev: (619.10, 689.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3378) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 302, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (283.91, 127.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 119285, 1, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 438, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (485.16, 245.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (311.87, 154.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 209, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (265.32, 122.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687324 reads in 125.243 CPU sec, 4.060 real sec [M::mem_process_seqs] Processed 287388 reads in 63.337 CPU sec, 2.053 real sec sorting block 110, #reads = 1080330 mem = 536878945 peak = 1077874688 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 120209008 overall: 69780213760 mem 19594.195 user 543.724 sys 676.696 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_5KC-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1136_5KC-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_5KC-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_5KC-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_5KC-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 676.731 sec; CPU: 20137.922 sec sorting block 111, #reads = 729059 mem = 362419717 peak = 1132920832 uniq header count 1 merging 112 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 6803387552 output reads: 120895253 bam_mem_sort: 112 calls 76.911 user 2.053 sys 79.631 real bam_write: 112 calls 51.840 user 3.625 sys 68.395 real execute: 1 calls 38.747 user 2.029 sys 733.921 real merge_files: 1 calls 38.711 user 1.996 sys 56.769 real parse_chunk: 1787 calls 100.602 user 27.283 sys 134.029 real read_chunk: 4823 calls 60.803 user 38.444 sys 604.003 real sort_block: 1 calls 40.530 user 37.430 sys 577.016 real write_chunk: 3034 calls 38.237 user 1.646 sys 55.626 real overall: 3088666624 mem 2196.346 user 98.250 sys 733.946 real ls: cannot access '*.cram': No such file or directory