cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 7774.700 CPU sec, 269.286 real sec
[M::process] read 678832 sequences (100000147 bp) in 2.249 CPU sec, 2.255 real sec...
[M::process] read 677380 sequences (100000004 bp) in 3.420 CPU sec, 3.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282353, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (362.05, 168.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677100 sequences (100000266 bp) in 3.378 CPU sec, 3.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280816, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 362, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (375.06, 171.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676726 sequences (100000094 bp) in 3.459 CPU sec, 3.553 real sec...
[M::mem_process_seqs] Processed 678832 reads in 332.599 CPU sec, 10.681 real sec
[M::mem_process_seqs] Processed 677380 reads in 173.327 CPU sec, 5.614 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280974, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 364, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (374.97, 169.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 0, #reads = 1066686 mem = 536879002 peak = 583778304
[M::process] read 676318 sequences (100000177 bp) in 3.305 CPU sec, 4.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281206, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 368, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (378.55, 170.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675966 sequences (100000260 bp) in 2.859 CPU sec, 2.867 real sec...
[M::mem_process_seqs] Processed 677100 reads in 285.798 CPU sec, 9.159 real sec
[M::mem_process_seqs] Processed 676726 reads in 167.352 CPU sec, 5.321 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280786, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 1, #reads = 1065901 mem = 536878600 peak = 843300864
[M::mem_pestat] (25, 50, 75) percentile: (247, 369, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (379.49, 169.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675884 sequences (100000061 bp) in 3.686 CPU sec, 4.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280896, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 254, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191)
[M::mem_pestat] mean and std.dev: (317.56, 155.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1522)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (382.60, 170.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676318 reads in 198.963 CPU sec, 6.415 real sec
[M::mem_process_seqs] Processed 675966 reads in 126.479 CPU sec, 4.026 real sec
sorting block 2, #reads = 1064375 mem = 536880383 peak = 843300864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281209, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (385.52, 171.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675442 sequences (100000090 bp) in 4.149 CPU sec, 4.474 real sec...
[M::mem_process_seqs] Processed 675884 reads in 105.113 CPU sec, 3.503 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281382, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 400, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (326.50, 88.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 901)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (389.01, 172.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675442 reads in 102.461 CPU sec, 3.492 real sec
[M::process] read 675490 sequences (100000188 bp) in 4.471 CPU sec, 4.837 real sec...
sorting block 3, #reads = 1064624 mem = 536879406 peak = 843300864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280997, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (390.92, 174.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675212 sequences (100000246 bp) in 3.143 CPU sec, 3.427 real sec...
[M::mem_process_seqs] Processed 675490 reads in 104.792 CPU sec, 3.512 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280808, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 379, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (388.16, 171.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675212 reads in 109.092 CPU sec, 3.646 real sec
[M::process] read 675102 sequences (100000141 bp) in 3.996 CPU sec, 4.434 real sec...
sorting block 4, #reads = 1064649 mem = 536875691 peak = 843300864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280194, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (392.98, 173.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675102 reads in 112.375 CPU sec, 3.782 real sec
[M::process] read 674946 sequences (100000022 bp) in 4.260 CPU sec, 4.716 real sec...
sorting block 5, #reads = 1065574 mem = 536879508 peak = 843300864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280050, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674854 sequences (100000245 bp) in 2.694 CPU sec, 2.953 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (257, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (392.15, 172.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674946 reads in 104.815 CPU sec, 3.511 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280238, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 388, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (396.05, 174.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 6, #reads = 1064815 mem = 536877337 peak = 858701824
[M::process] read 674572 sequences (100000078 bp) in 3.388 CPU sec, 3.701 real sec...
[M::mem_process_seqs] Processed 674854 reads in 108.195 CPU sec, 3.606 real sec
[M::process] read 674740 sequences (100000035 bp) in 2.670 CPU sec, 2.920 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280258, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (394.43, 173.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674572 reads in 104.054 CPU sec, 3.448 real sec
sorting block 7, #reads = 1064787 mem = 536878851 peak = 858701824
[M::process] read 674570 sequences (100000236 bp) in 3.009 CPU sec, 3.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280103, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (394.54, 173.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280699, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (393.91, 173.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674740 reads in 209.275 CPU sec, 6.727 real sec
[M::process] read 674658 sequences (100000007 bp) in 3.664 CPU sec, 3.970 real sec...
[M::mem_process_seqs] Processed 674570 reads in 131.921 CPU sec, 4.208 real sec
sorting block 8, #reads = 1063784 mem = 536878172 peak = 858701824
[M::process] read 674496 sequences (100000116 bp) in 2.621 CPU sec, 2.822 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280830, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 381, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (389.41, 170.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674658 reads in 109.667 CPU sec, 3.649 real sec
sorting block 9, #reads = 1063657 mem = 536879141 peak = 858701824
[M::process] read 674590 sequences (100000283 bp) in 3.042 CPU sec, 3.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280195, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (390.13, 170.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674496 reads in 109.900 CPU sec, 3.652 real sec
[M::process] read 674854 sequences (100000145 bp) in 3.075 CPU sec, 3.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279784, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 377, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (387.33, 169.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 10, #reads = 1064613 mem = 536878406 peak = 858701824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280530, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 372, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (382.75, 167.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674590 reads in 202.382 CPU sec, 6.584 real sec
[M::process] read 674836 sequences (100000172 bp) in 3.182 CPU sec, 3.641 real sec...
[M::mem_process_seqs] Processed 674854 reads in 125.850 CPU sec, 4.121 real sec
sorting block 11, #reads = 1063273 mem = 536878131 peak = 858701824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280358, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 374, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (383.72, 168.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674852 sequences (100000157 bp) in 3.075 CPU sec, 3.383 real sec...
[M::mem_process_seqs] Processed 674836 reads in 106.983 CPU sec, 3.571 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280510, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 370, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (380.63, 166.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675090 sequences (100000022 bp) in 3.065 CPU sec, 3.371 real sec...
[M::mem_process_seqs] Processed 674852 reads in 109.002 CPU sec, 3.614 real sec
sorting block 12, #reads = 1063884 mem = 536880816 peak = 858701824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280345, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 372, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (381.78, 167.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674940 sequences (100000150 bp) in 3.106 CPU sec, 3.468 real sec...
[M::mem_process_seqs] Processed 675090 reads in 103.581 CPU sec, 3.462 real sec
[M::process] read 674930 sequences (100000004 bp) in 3.024 CPU sec, 3.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280655, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 371, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (381.66, 167.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1063713 mem = 536879494 peak = 872853504
[M::mem_process_seqs] Processed 674940 reads in 111.416 CPU sec, 3.711 real sec
[M::process] read 674888 sequences (100000272 bp) in 2.616 CPU sec, 3.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280736, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 373, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (383.08, 168.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674930 reads in 106.032 CPU sec, 3.573 real sec
sorting block 14, #reads = 1064381 mem = 536880395 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281278, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 374, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (384.36, 168.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (431, 500, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (163, 833)
[M::mem_pestat] mean and std.dev: (498.67, 54.71)
[M::mem_pestat] low and high boundaries for proper pairs: (29, 967)
[M::mem_pestat] skip orientation RR
[M::process] read 674778 sequences (100000136 bp) in 2.978 CPU sec, 3.376 real sec...
[M::mem_process_seqs] Processed 674888 reads in 110.570 CPU sec, 3.723 real sec
[M::process] read 674478 sequences (100000054 bp) in 2.737 CPU sec, 3.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280776, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 376, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (385.45, 168.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674778 reads in 106.451 CPU sec, 3.586 real sec
sorting block 15, #reads = 1062798 mem = 536879500 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280844, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 381, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (390.93, 170.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674204 sequences (100000114 bp) in 2.843 CPU sec, 3.340 real sec...
[M::mem_process_seqs] Processed 674478 reads in 107.703 CPU sec, 3.642 real sec
sorting block 16, #reads = 1063277 mem = 536879805 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281195, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 384, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (392.91, 171.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676090 sequences (100000008 bp) in 2.973 CPU sec, 3.289 real sec...
[M::mem_process_seqs] Processed 674204 reads in 104.079 CPU sec, 3.484 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281637, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677012 sequences (100000283 bp) in 2.776 CPU sec, 3.182 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (247, 370, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (380.72, 170.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676090 reads in 110.191 CPU sec, 3.783 real sec
sorting block 17, #reads = 1062968 mem = 536877887 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281061, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 365, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (377.17, 172.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 321, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (281.83, 97.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RR
[M::process] read 676790 sequences (100000085 bp) in 3.073 CPU sec, 3.407 real sec...
[M::mem_process_seqs] Processed 677012 reads in 104.593 CPU sec, 3.509 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280954, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 366, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (378.05, 171.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676566 sequences (100000070 bp) in 2.893 CPU sec, 3.283 real sec...
[M::mem_process_seqs] Processed 676790 reads in 106.568 CPU sec, 3.599 real sec
sorting block 18, #reads = 1062918 mem = 536880417 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280379, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 370, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (380.58, 172.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676566 reads in 103.896 CPU sec, 3.513 real sec
[M::process] read 676170 sequences (100000267 bp) in 3.049 CPU sec, 3.545 real sec...
sorting block 19, #reads = 1067702 mem = 536880645 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280698, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (382.90, 171.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676156 sequences (100000267 bp) in 2.991 CPU sec, 3.457 real sec...
[M::mem_process_seqs] Processed 676170 reads in 108.817 CPU sec, 3.817 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281097, 1, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 274, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (31, 436)
[M::mem_pestat] mean and std.dev: (241.00, 63.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 517)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (383.97, 171.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675924 sequences (100000094 bp) in 2.795 CPU sec, 3.096 real sec...
sorting block 20, #reads = 1065888 mem = 536880182 peak = 872853504
[M::mem_process_seqs] Processed 676156 reads in 105.388 CPU sec, 3.562 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281005, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (385.26, 171.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (296, 340, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331)
[M::mem_pestat] mean and std.dev: (449.00, 260.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1676)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675924 reads in 110.654 CPU sec, 3.738 real sec
[M::process] read 675660 sequences (100000235 bp) in 3.343 CPU sec, 4.016 real sec...
sorting block 21, #reads = 1066443 mem = 536879068 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281127, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 381, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (388.67, 172.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675534 sequences (100000090 bp) in 3.084 CPU sec, 3.380 real sec...
[M::mem_process_seqs] Processed 675660 reads in 105.859 CPU sec, 3.554 real sec
[M::process] read 675464 sequences (100000126 bp) in 2.700 CPU sec, 3.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280516, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (389.04, 172.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675534 reads in 109.163 CPU sec, 3.666 real sec
sorting block 22, #reads = 1065842 mem = 536876539 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281180, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 382, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (389.61, 172.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675046 sequences (100000111 bp) in 2.790 CPU sec, 3.223 real sec...
[M::mem_process_seqs] Processed 675464 reads in 102.536 CPU sec, 3.473 real sec
sorting block 23, #reads = 1065482 mem = 536879516 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280312, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (389.74, 171.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674892 sequences (100000282 bp) in 2.805 CPU sec, 3.121 real sec...
[M::mem_process_seqs] Processed 675046 reads in 107.818 CPU sec, 3.682 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280859, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 383, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (390.30, 170.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674724 sequences (100000210 bp) in 2.968 CPU sec, 3.461 real sec...
[M::mem_process_seqs] Processed 674892 reads in 103.674 CPU sec, 3.493 real sec
sorting block 24, #reads = 1065947 mem = 536879160 peak = 872853504
[M::process] read 674746 sequences (100000224 bp) in 2.683 CPU sec, 3.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280679, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 388, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (395.20, 173.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674724 reads in 108.510 CPU sec, 3.684 real sec
sorting block 25, #reads = 1066359 mem = 536879473 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280162, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (417, 4142, 4142)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11592)
[M::mem_pestat] mean and std.dev: (2427.73, 1878.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15317)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 387, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (393.93, 172.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674600 sequences (100000234 bp) in 2.797 CPU sec, 3.229 real sec...
[M::mem_process_seqs] Processed 674746 reads in 106.523 CPU sec, 3.602 real sec
[M::process] read 674534 sequences (100000204 bp) in 2.696 CPU sec, 2.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280498, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 387, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (394.03, 172.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674600 reads in 107.860 CPU sec, 3.634 real sec
sorting block 26, #reads = 1064633 mem = 536877474 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280192, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 386, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (393.04, 170.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674224 sequences (100000112 bp) in 3.070 CPU sec, 3.408 real sec...
[M::mem_process_seqs] Processed 674534 reads in 108.966 CPU sec, 3.638 real sec
[M::process] read 674566 sequences (100000260 bp) in 2.701 CPU sec, 2.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280336, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 391, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (397.41, 173.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1064059 mem = 536882614 peak = 872853504
[M::mem_process_seqs] Processed 674224 reads in 112.458 CPU sec, 3.791 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280398, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 379, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (387.76, 169.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674646 sequences (100000121 bp) in 3.015 CPU sec, 3.357 real sec...
[M::mem_process_seqs] Processed 674566 reads in 112.549 CPU sec, 3.847 real sec
sorting block 28, #reads = 1064357 mem = 536879270 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280789, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 374, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (384.05, 167.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674576 sequences (100000225 bp) in 2.984 CPU sec, 3.359 real sec...
[M::mem_process_seqs] Processed 674646 reads in 108.214 CPU sec, 3.624 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280882, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (385.12, 167.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674592 sequences (100000117 bp) in 2.939 CPU sec, 3.254 real sec...
[M::mem_process_seqs] Processed 674576 reads in 110.898 CPU sec, 3.723 real sec
sorting block 29, #reads = 1063467 mem = 536878357 peak = 872853504
[M::process] read 674810 sequences (100000130 bp) in 2.549 CPU sec, 2.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280774, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (384.86, 167.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 30, #reads = 1063405 mem = 536880002 peak = 872853504
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280559, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 373, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (382.44, 167.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675100 sequences (100000244 bp) in 3.001 CPU sec, 3.298 real sec...
[M::mem_process_seqs] Processed 674592 reads in 205.438 CPU sec, 6.706 real sec
[M::mem_process_seqs] Processed 674810 reads in 128.483 CPU sec, 4.127 real sec
[M::process] read 674624 sequences (100000043 bp) in 2.747 CPU sec, 3.121 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281025, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 374, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (383.42, 168.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675100 reads in 109.425 CPU sec, 3.743 real sec
sorting block 31, #reads = 1063694 mem = 536876492 peak = 880865280
[M::process] read 674868 sequences (100000297 bp) in 2.576 CPU sec, 2.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280666, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (385.24, 168.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674624 reads in 112.848 CPU sec, 3.868 real sec
sorting block 32, #reads = 1064171 mem = 536877973 peak = 904220672
[M::process] read 674982 sequences (100000129 bp) in 3.025 CPU sec, 3.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280527, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 376, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (385.75, 169.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674868 reads in 107.238 CPU sec, 3.595 real sec
[M::process] read 675048 sequences (100000193 bp) in 2.884 CPU sec, 3.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280345, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 375, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (384.64, 168.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674982 reads in 111.084 CPU sec, 3.752 real sec
sorting block 33, #reads = 1063543 mem = 536877563 peak = 904220672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280997, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 375, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (385.27, 169.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674816 sequences (100000082 bp) in 2.867 CPU sec, 3.338 real sec...
[M::mem_process_seqs] Processed 675048 reads in 107.295 CPU sec, 3.616 real sec
[M::process] read 674828 sequences (100000059 bp) in 2.736 CPU sec, 2.991 real sec...
sorting block 34, #reads = 1063394 mem = 536880136 peak = 935895040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281105, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 378, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (387.80, 170.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674690 sequences (100000140 bp) in 2.775 CPU sec, 3.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281898, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (390.63, 172.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674828 reads in 109.196 CPU sec, 3.534 real sec
sorting block 35, #reads = 1063867 mem = 536883520 peak = 935895040
[M::process] read 676088 sequences (100000033 bp) in 2.703 CPU sec, 2.930 real sec...
[M::mem_process_seqs] Processed 674816 reads in 317.022 CPU sec, 10.366 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280697, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (393.76, 173.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281521, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 370, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (381.62, 172.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677682 sequences (100000099 bp) in 3.113 CPU sec, 3.413 real sec...
[M::mem_process_seqs] Processed 674690 reads in 210.354 CPU sec, 6.704 real sec
sorting block 36, #reads = 1062807 mem = 536876360 peak = 935895040
[M::process] read 677610 sequences (100000014 bp) in 2.595 CPU sec, 2.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281467, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 360, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (371.82, 170.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677682 reads in 115.015 CPU sec, 3.664 real sec
[M::process] read 677330 sequences (100000117 bp) in 2.605 CPU sec, 2.770 real sec...
sorting block 37, #reads = 1063966 mem = 536878878 peak = 947752960
[M::mem_process_seqs] Processed 676088 reads in 335.829 CPU sec, 10.714 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281561, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 362, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (373.73, 171.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281427, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 363, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (374.44, 170.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677610 reads in 219.101 CPU sec, 6.990 real sec
[M::process] read 677080 sequences (100000029 bp) in 3.195 CPU sec, 3.559 real sec...
[M::mem_process_seqs] Processed 677330 reads in 124.056 CPU sec, 3.979 real sec
sorting block 38, #reads = 1064992 mem = 536878467 peak = 947752960
[M::process] read 677052 sequences (100000123 bp) in 2.610 CPU sec, 2.863 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281225, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 366, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (376.45, 170.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677080 reads in 112.456 CPU sec, 3.837 real sec
sorting block 39, #reads = 1066512 mem = 536886055 peak = 947752960
[M::process] read 676536 sequences (100000145 bp) in 2.739 CPU sec, 3.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281510, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 368, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (378.03, 170.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281914, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 371, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (380.93, 171.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676458 sequences (100000131 bp) in 2.751 CPU sec, 3.299 real sec...
[M::mem_process_seqs] Processed 677052 reads in 202.869 CPU sec, 6.581 real sec
[M::mem_process_seqs] Processed 676536 reads in 129.931 CPU sec, 4.188 real sec
sorting block 40, #reads = 1066269 mem = 536879322 peak = 947752960
[M::process] read 676054 sequences (100000108 bp) in 2.911 CPU sec, 3.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281172, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (383.38, 171.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676458 reads in 106.492 CPU sec, 3.567 real sec
[M::process] read 676052 sequences (100000025 bp) in 2.704 CPU sec, 3.067 real sec...
sorting block 41, #reads = 1066137 mem = 536878955 peak = 947752960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281125, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (384.95, 171.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675750 sequences (100000229 bp) in 2.700 CPU sec, 2.963 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280911, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (384.56, 171.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676054 reads in 200.509 CPU sec, 6.529 real sec
[M::mem_process_seqs] Processed 676052 reads in 124.133 CPU sec, 3.985 real sec
sorting block 42, #reads = 1066033 mem = 536879432 peak = 947752960
[M::process] read 675166 sequences (100000248 bp) in 2.824 CPU sec, 3.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280836, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (84, 173, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (197.00, 117.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (387.98, 172.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675166 sequences (100000115 bp) in 2.711 CPU sec, 3.042 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280664, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 381, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (388.80, 171.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675750 reads in 200.943 CPU sec, 6.537 real sec
sorting block 43, #reads = 1066075 mem = 536879876 peak = 947752960
[M::mem_process_seqs] Processed 675166 reads in 126.098 CPU sec, 4.102 real sec
[M::process] read 675388 sequences (100000242 bp) in 2.785 CPU sec, 3.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281041, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (393.64, 175.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1064981 mem = 536879200 peak = 947752960
[M::process] read 675042 sequences (100000259 bp) in 2.648 CPU sec, 2.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281084, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (393.01, 174.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675166 reads in 203.521 CPU sec, 6.621 real sec
[M::mem_process_seqs] Processed 675388 reads in 128.906 CPU sec, 4.152 real sec
[M::process] read 675182 sequences (100000192 bp) in 2.335 CPU sec, 2.703 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280685, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (392.66, 173.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1064672 mem = 536877217 peak = 947752960
[M::process] read 674872 sequences (100000122 bp) in 2.747 CPU sec, 3.061 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280474, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (391.57, 172.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675042 reads in 205.035 CPU sec, 6.637 real sec
[M::mem_process_seqs] Processed 675182 reads in 129.242 CPU sec, 4.158 real sec
[M::process] read 675862 sequences (100000001 bp) in 2.640 CPU sec, 2.919 real sec...
sorting block 46, #reads = 1065192 mem = 536879731 peak = 947752960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279712, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 386, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (393.75, 172.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675646 sequences (100000027 bp) in 2.800 CPU sec, 3.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281134, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 366, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (376.77, 167.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674872 reads in 203.237 CPU sec, 6.624 real sec
[M::mem_process_seqs] Processed 675862 reads in 126.399 CPU sec, 4.090 real sec
sorting block 47, #reads = 1064562 mem = 536878103 peak = 957485056
[M::process] read 675176 sequences (100000260 bp) in 2.948 CPU sec, 3.250 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280598, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 371, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (382.22, 170.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674428 sequences (100000025 bp) in 2.436 CPU sec, 2.641 real sec...
sorting block 48, #reads = 1065097 mem = 536879345 peak = 973643776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281191, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 376, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (386.50, 171.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675646 reads in 206.570 CPU sec, 6.685 real sec
[M::mem_process_seqs] Processed 675176 reads in 126.553 CPU sec, 4.059 real sec
[M::process] read 674498 sequences (100000036 bp) in 2.357 CPU sec, 2.703 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280022, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (393.40, 171.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1064805 mem = 536876113 peak = 973643776
[M::process] read 674578 sequences (100000076 bp) in 2.852 CPU sec, 3.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280229, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (392.26, 171.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674428 reads in 206.727 CPU sec, 6.722 real sec
[M::mem_process_seqs] Processed 674498 reads in 125.475 CPU sec, 4.034 real sec
[M::process] read 674502 sequences (100000049 bp) in 2.553 CPU sec, 2.914 real sec...
sorting block 50, #reads = 1064105 mem = 536879273 peak = 973643776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279807, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (391.27, 171.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674686 sequences (100000265 bp) in 2.520 CPU sec, 2.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280421, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (393.75, 172.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674578 reads in 203.520 CPU sec, 6.569 real sec
[M::mem_process_seqs] Processed 674502 reads in 125.082 CPU sec, 3.990 real sec
[M::process] read 674788 sequences (100000275 bp) in 2.433 CPU sec, 2.739 real sec...
sorting block 51, #reads = 1063339 mem = 536878173 peak = 973643776
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280291, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (391.69, 172.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674670 sequences (100000274 bp) in 2.935 CPU sec, 3.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280427, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 381, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (390.85, 172.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674686 reads in 202.026 CPU sec, 6.524 real sec
sorting block 52, #reads = 1064567 mem = 536877351 peak = 973643776
[M::mem_process_seqs] Processed 674788 reads in 126.526 CPU sec, 4.022 real sec
[M::process] read 675072 sequences (100000238 bp) in 2.360 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281056, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (391.37, 172.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1063131 mem = 536877781 peak = 1003012096
[M::process] read 674066 sequences (100000135 bp) in 3.084 CPU sec, 3.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281497, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (389.91, 174.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674670 reads in 201.272 CPU sec, 6.518 real sec
[M::mem_process_seqs] Processed 675072 reads in 125.762 CPU sec, 4.031 real sec
[M::process] read 673996 sequences (100000233 bp) in 2.384 CPU sec, 2.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280549, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 390, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (400.08, 176.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1062931 mem = 536878873 peak = 1003012096
[M::process] read 677152 sequences (100000053 bp) in 2.815 CPU sec, 3.109 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280846, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 393, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (402.22, 177.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674066 reads in 197.611 CPU sec, 6.376 real sec
[M::mem_process_seqs] Processed 673996 reads in 124.836 CPU sec, 3.980 real sec
[M::process] read 677538 sequences (100000255 bp) in 2.596 CPU sec, 2.846 real sec...
sorting block 55, #reads = 1063076 mem = 536879362 peak = 1003012096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280994, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 363, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (376.61, 172.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677250 sequences (100000014 bp) in 2.713 CPU sec, 3.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280526, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 364, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (375.89, 171.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677152 reads in 204.735 CPU sec, 6.625 real sec
[M::mem_process_seqs] Processed 677538 reads in 127.619 CPU sec, 4.091 real sec
sorting block 56, #reads = 1063437 mem = 536877650 peak = 1003012096
[M::process] read 676866 sequences (100000127 bp) in 2.740 CPU sec, 2.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280312, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 365, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (376.37, 171.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676714 sequences (100000197 bp) in 2.794 CPU sec, 3.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280320, 1, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (99, 341, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (247.60, 115.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 364, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (375.52, 169.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (293, 293, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (251, 356)
[M::mem_pestat] mean and std.dev: (300.64, 10.10)
[M::mem_pestat] low and high boundaries for proper pairs: (230, 377)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677250 reads in 198.845 CPU sec, 6.406 real sec
sorting block 57, #reads = 1067492 mem = 536880366 peak = 1003012096
[M::mem_process_seqs] Processed 676866 reads in 123.809 CPU sec, 3.930 real sec
[M::process] read 676732 sequences (100000018 bp) in 2.455 CPU sec, 2.661 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280581, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 367, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (377.68, 170.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 292, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207)
[M::mem_pestat] mean and std.dev: (348.75, 214.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1533)
[M::mem_pestat] skip orientation RR
sorting block 58, #reads = 1066828 mem = 536882239 peak = 1003012096
[M::process] read 676690 sequences (100000194 bp) in 2.739 CPU sec, 3.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280643, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 367, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (378.82, 172.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676714 reads in 200.682 CPU sec, 6.474 real sec
[M::mem_process_seqs] Processed 676732 reads in 126.648 CPU sec, 4.037 real sec
[M::process] read 676266 sequences (100000154 bp) in 2.351 CPU sec, 2.562 real sec...
sorting block 59, #reads = 1066836 mem = 536881304 peak = 1003012096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280495, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 371, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (381.56, 173.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 359, 8841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 26217)
[M::mem_pestat] mean and std.dev: (3220.42, 4004.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 34905)
[M::mem_pestat] skip orientation RR
[M::process] read 676204 sequences (100000247 bp) in 2.596 CPU sec, 2.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280973, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 373, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (383.17, 172.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676690 reads in 200.240 CPU sec, 6.478 real sec
[M::mem_process_seqs] Processed 676266 reads in 124.072 CPU sec, 4.011 real sec
[M::process] read 676118 sequences (100000135 bp) in 2.303 CPU sec, 2.435 real sec...
sorting block 60, #reads = 1065820 mem = 536878795 peak = 1003012096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280163, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 373, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (382.66, 171.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675912 sequences (100000138 bp) in 3.111 CPU sec, 3.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280652, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 374, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (383.48, 171.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676204 reads in 198.777 CPU sec, 6.413 real sec
[M::mem_process_seqs] Processed 676118 reads in 122.071 CPU sec, 3.890 real sec
sorting block 61, #reads = 1066284 mem = 536880112 peak = 1003012096
[M::process] read 675466 sequences (100000155 bp) in 2.852 CPU sec, 3.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280159, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 377, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (386.60, 173.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 62, #reads = 1065177 mem = 536877507 peak = 1003012096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280367, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (388.65, 173.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675428 sequences (100000088 bp) in 3.335 CPU sec, 3.880 real sec...
[M::mem_process_seqs] Processed 675912 reads in 195.711 CPU sec, 6.339 real sec
[M::mem_process_seqs] Processed 675466 reads in 119.674 CPU sec, 3.825 real sec
[M::process] read 675354 sequences (100000278 bp) in 2.856 CPU sec, 3.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280181, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (389.00, 172.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1065472 mem = 536883290 peak = 1003012096
[M::process] read 675454 sequences (100000295 bp) in 2.894 CPU sec, 3.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280371, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (392.21, 174.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675428 reads in 199.443 CPU sec, 6.461 real sec
[M::mem_process_seqs] Processed 675354 reads in 122.886 CPU sec, 3.951 real sec
[M::process] read 675058 sequences (100000158 bp) in 2.577 CPU sec, 2.850 real sec...
sorting block 64, #reads = 1065723 mem = 536880368 peak = 1003012096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280030, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (391.13, 173.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675168 sequences (100000138 bp) in 2.992 CPU sec, 3.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279922, 0, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (392.66, 174.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (358, 358, 609)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111)
[M::mem_pestat] mean and std.dev: (429.86, 175.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1362)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675454 reads in 205.807 CPU sec, 6.625 real sec
[M::mem_process_seqs] Processed 675058 reads in 127.158 CPU sec, 4.045 real sec
sorting block 65, #reads = 1064243 mem = 536878210 peak = 1003012096
[M::process] read 674890 sequences (100000080 bp) in 3.005 CPU sec, 3.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280918, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (391.47, 172.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675026 sequences (100000140 bp) in 3.089 CPU sec, 3.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280234, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (391.58, 171.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675168 reads in 203.287 CPU sec, 6.550 real sec
sorting block 66, #reads = 1064119 mem = 536880847 peak = 1003012096
[M::mem_process_seqs] Processed 674890 reads in 128.124 CPU sec, 4.079 real sec
[M::process] read 674690 sequences (100000199 bp) in 2.684 CPU sec, 2.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280338, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (388.44, 170.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1064582 mem = 536877213 peak = 1016147968
[M::process] read 674522 sequences (100000071 bp) in 3.182 CPU sec, 3.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280222, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 383, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (390.11, 169.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 319, 802)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1940)
[M::mem_pestat] mean and std.dev: (397.69, 296.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2509)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675026 reads in 203.312 CPU sec, 6.547 real sec
[M::mem_process_seqs] Processed 674690 reads in 128.505 CPU sec, 4.098 real sec
[M::process] read 674434 sequences (100000263 bp) in 3.170 CPU sec, 3.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280587, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (392.37, 170.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1064072 mem = 536879063 peak = 1016147968
[M::process] read 674242 sequences (100000234 bp) in 3.168 CPU sec, 3.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280305, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (391.24, 169.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674522 reads in 201.290 CPU sec, 6.503 real sec
[M::mem_process_seqs] Processed 674434 reads in 128.143 CPU sec, 4.108 real sec
[M::process] read 674332 sequences (100000128 bp) in 2.126 CPU sec, 2.330 real sec...
sorting block 69, #reads = 1064768 mem = 536879977 peak = 1016147968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280375, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 384, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (391.44, 169.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674266 sequences (100000039 bp) in 2.724 CPU sec, 3.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280643, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 383, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (390.38, 168.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674242 reads in 202.635 CPU sec, 6.514 real sec
[M::mem_process_seqs] Processed 674332 reads in 126.861 CPU sec, 4.053 real sec
sorting block 70, #reads = 1063349 mem = 536879359 peak = 1016147968
[M::process] read 674480 sequences (100000205 bp) in 2.694 CPU sec, 2.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279519, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 386, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (393.40, 170.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674302 sequences (100000245 bp) in 3.267 CPU sec, 3.617 real sec...
sorting block 71, #reads = 1063849 mem = 536878977 peak = 1016147968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280378, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (360, 403, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (274, 489)
[M::mem_pestat] mean and std.dev: (390.10, 19.71)
[M::mem_pestat] low and high boundaries for proper pairs: (231, 532)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 383, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (390.91, 169.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674266 reads in 200.118 CPU sec, 6.452 real sec
[M::mem_process_seqs] Processed 674480 reads in 124.870 CPU sec, 3.970 real sec
[M::process] read 674622 sequences (100000025 bp) in 2.656 CPU sec, 2.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280412, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 381, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (389.15, 168.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 72, #reads = 1063310 mem = 536877993 peak = 1016147968
[M::process] read 674558 sequences (100000298 bp) in 3.052 CPU sec, 3.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280418, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 380, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (388.08, 169.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674302 reads in 203.201 CPU sec, 6.532 real sec
[M::mem_process_seqs] Processed 674622 reads in 125.046 CPU sec, 3.971 real sec
[M::process] read 674380 sequences (100000267 bp) in 2.718 CPU sec, 2.864 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281357, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 378, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (387.74, 169.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 73, #reads = 1063876 mem = 536878923 peak = 1016147968
[M::process] read 677454 sequences (100000052 bp) in 3.212 CPU sec, 3.750 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280555, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 382, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (390.71, 170.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674558 reads in 199.715 CPU sec, 6.450 real sec
[M::mem_process_seqs] Processed 674380 reads in 126.181 CPU sec, 4.013 real sec
sorting block 74, #reads = 1063775 mem = 536879354 peak = 1016147968
[M::process] read 679776 sequences (100000049 bp) in 2.603 CPU sec, 2.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282414, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 358, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (371.80, 171.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679070 sequences (100000112 bp) in 3.016 CPU sec, 3.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282986, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 346, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (361.96, 170.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677454 reads in 217.484 CPU sec, 6.983 real sec
[M::mem_process_seqs] Processed 679776 reads in 138.240 CPU sec, 4.402 real sec
[M::process] read 679074 sequences (100000139 bp) in 2.272 CPU sec, 2.352 real sec...
sorting block 75, #reads = 1062730 mem = 536880252 peak = 1016147968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283269, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 352, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (366.64, 172.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 76, #reads = 1063059 mem = 536881149 peak = 1016147968
[M::process] read 677712 sequences (100000063 bp) in 3.424 CPU sec, 3.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282448, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 350, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (364.46, 170.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679070 reads in 217.953 CPU sec, 7.020 real sec
[M::mem_process_seqs] Processed 679074 reads in 137.180 CPU sec, 4.373 real sec
[M::process] read 677054 sequences (100000133 bp) in 2.438 CPU sec, 2.542 real sec...
sorting block 77, #reads = 1062721 mem = 536879779 peak = 1016147968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282306, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 360, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (372.92, 171.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676730 sequences (100000140 bp) in 2.960 CPU sec, 3.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281563, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 369, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (379.32, 172.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 322, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (272.91, 58.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 670)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677712 reads in 202.584 CPU sec, 6.568 real sec
[M::mem_process_seqs] Processed 677054 reads in 127.530 CPU sec, 4.157 real sec
[M::process] read 676434 sequences (100000247 bp) in 2.079 CPU sec, 2.278 real sec...
sorting block 78, #reads = 1063071 mem = 536878076 peak = 1016147968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282173, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 374, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (383.63, 174.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (419, 685, 924)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934)
[M::mem_pestat] mean and std.dev: (555.08, 276.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2439)
[M::mem_pestat] skip orientation RR
[M::process] read 676146 sequences (100000190 bp) in 3.310 CPU sec, 3.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281452, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 377, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (385.61, 174.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676730 reads in 203.714 CPU sec, 6.622 real sec
[M::mem_process_seqs] Processed 676434 reads in 125.715 CPU sec, 4.015 real sec
sorting block 79, #reads = 1062937 mem = 536880555 peak = 1016147968
[M::process] read 675952 sequences (100000173 bp) in 2.458 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281640, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (387.95, 174.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676170 sequences (100000222 bp) in 2.968 CPU sec, 3.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281388, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (388.84, 174.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676146 reads in 209.340 CPU sec, 6.753 real sec
sorting block 80, #reads = 1063243 mem = 536877357 peak = 1019736064
[M::mem_process_seqs] Processed 675952 reads in 128.205 CPU sec, 4.090 real sec
[M::process] read 675576 sequences (100000194 bp) in 2.503 CPU sec, 2.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281241, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (385.73, 172.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (687, 799, 799)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (463, 1023)
[M::mem_pestat] mean and std.dev: (774.00, 50.47)
[M::mem_pestat] low and high boundaries for proper pairs: (351, 1135)
[M::mem_pestat] skip orientation RR
sorting block 81, #reads = 1063232 mem = 536878577 peak = 1048084480
[M::process] read 675590 sequences (100000204 bp) in 3.455 CPU sec, 3.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281309, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (393.56, 175.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676170 reads in 213.938 CPU sec, 6.887 real sec
[M::mem_process_seqs] Processed 675576 reads in 134.026 CPU sec, 4.250 real sec
[M::process] read 675732 sequences (100000092 bp) in 2.394 CPU sec, 2.519 real sec...
sorting block 82, #reads = 1061883 mem = 536878135 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281139, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (394.08, 175.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675572 sequences (100000134 bp) in 3.270 CPU sec, 3.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281684, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 386, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (392.48, 175.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1323)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (412.69, 227.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675590 reads in 212.019 CPU sec, 6.772 real sec
[M::mem_process_seqs] Processed 675732 reads in 132.517 CPU sec, 4.185 real sec
[M::process] read 675626 sequences (100000228 bp) in 2.423 CPU sec, 2.477 real sec...
sorting block 83, #reads = 1062194 mem = 536879386 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281222, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (388.56, 172.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675268 sequences (100000007 bp) in 3.630 CPU sec, 3.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281342, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 379, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (387.18, 171.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675572 reads in 219.697 CPU sec, 7.024 real sec
[M::mem_process_seqs] Processed 675626 reads in 138.433 CPU sec, 4.375 real sec
[M::process] read 675062 sequences (100000089 bp) in 2.255 CPU sec, 2.308 real sec...
sorting block 84, #reads = 1062089 mem = 536879563 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280892, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (389.16, 171.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 85, #reads = 1060153 mem = 536882617 peak = 1048084480
[M::process] read 675008 sequences (100000064 bp) in 3.662 CPU sec, 3.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281333, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (390.01, 172.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675268 reads in 221.954 CPU sec, 7.086 real sec
[M::process] read 675034 sequences (100000281 bp) in 2.334 CPU sec, 2.369 real sec...
[M::mem_process_seqs] Processed 675062 reads in 137.398 CPU sec, 4.336 real sec
sorting block 86, #reads = 1060050 mem = 536878022 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281288, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (389.50, 171.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675112 sequences (100000205 bp) in 3.635 CPU sec, 3.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281183, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (389.57, 172.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675008 reads in 221.059 CPU sec, 7.035 real sec
[M::process] read 675304 sequences (100000067 bp) in 2.412 CPU sec, 2.424 real sec...
[M::mem_process_seqs] Processed 675034 reads in 140.321 CPU sec, 4.423 real sec
sorting block 87, #reads = 1060978 mem = 536878677 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281104, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (387.36, 170.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675408 sequences (100000175 bp) in 3.611 CPU sec, 3.833 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280905, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (386.65, 170.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675112 reads in 221.593 CPU sec, 7.058 real sec
[M::process] read 675214 sequences (100000087 bp) in 2.439 CPU sec, 2.447 real sec...
[M::mem_process_seqs] Processed 675304 reads in 139.161 CPU sec, 4.389 real sec
sorting block 88, #reads = 1061185 mem = 536877366 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280933, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (386.42, 171.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675216 sequences (100000145 bp) in 3.718 CPU sec, 3.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281140, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 379, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (388.11, 172.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (491, 752, 752)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1274)
[M::mem_pestat] mean and std.dev: (584.40, 206.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1535)
[M::mem_pestat] skip orientation RR
[M::process] read 675058 sequences (100000296 bp) in 2.338 CPU sec, 2.338 real sec...
[M::mem_process_seqs] Processed 675408 reads in 226.976 CPU sec, 7.217 real sec
sorting block 89, #reads = 1060014 mem = 536889771 peak = 1048084480
[M::mem_process_seqs] Processed 675214 reads in 140.780 CPU sec, 4.449 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280864, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 379, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (388.16, 171.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 90, #reads = 1059336 mem = 536881315 peak = 1048084480
[M::process] read 675126 sequences (100000139 bp) in 3.673 CPU sec, 3.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280888, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (271, 338, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (97, 532)
[M::mem_pestat] mean and std.dev: (301.70, 57.34)
[M::mem_pestat] low and high boundaries for proper pairs: (10, 619)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (388.57, 171.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675216 reads in 220.710 CPU sec, 7.035 real sec
[M::process] read 675234 sequences (100000209 bp) in 2.443 CPU sec, 2.471 real sec...
[M::mem_process_seqs] Processed 675058 reads in 139.236 CPU sec, 4.391 real sec
sorting block 91, #reads = 1060104 mem = 536876584 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281360, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (389.41, 172.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680422 sequences (100000169 bp) in 3.697 CPU sec, 3.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281582, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (389.84, 173.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675126 reads in 224.876 CPU sec, 7.164 real sec
[M::process] read 681452 sequences (100000229 bp) in 2.496 CPU sec, 2.505 real sec...
[M::mem_process_seqs] Processed 675234 reads in 139.427 CPU sec, 4.394 real sec
sorting block 92, #reads = 1060158 mem = 536879702 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283730, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (351.89, 164.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680424 sequences (100000257 bp) in 2.940 CPU sec, 3.106 real sec...
[M::process] read 680242 sequences (100000023 bp) in 2.240 CPU sec, 2.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284858, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (346.06, 161.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680422 reads in 239.388 CPU sec, 7.656 real sec
[M::mem_process_seqs] Processed 681452 reads in 146.607 CPU sec, 4.655 real sec
sorting block 93, #reads = 1058796 mem = 536879767 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283549, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 339, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (352.35, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 94, #reads = 1059897 mem = 536880543 peak = 1048084480
[M::process] read 679560 sequences (100000029 bp) in 3.782 CPU sec, 3.963 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284292, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (349.66, 160.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678962 sequences (100000296 bp) in 2.493 CPU sec, 2.502 real sec...
[M::mem_process_seqs] Processed 680424 reads in 236.282 CPU sec, 7.527 real sec
[M::mem_process_seqs] Processed 680242 reads in 147.467 CPU sec, 4.661 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283855, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 345, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (355.06, 160.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 95, #reads = 1058495 mem = 536877363 peak = 1048084480
[M::process] read 678490 sequences (100000029 bp) in 3.674 CPU sec, 3.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283355, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 349, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (359.67, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679560 reads in 225.649 CPU sec, 7.220 real sec
[M::process] read 677922 sequences (100000218 bp) in 2.356 CPU sec, 2.374 real sec...
[M::mem_process_seqs] Processed 678962 reads in 139.526 CPU sec, 4.410 real sec
sorting block 96, #reads = 1058442 mem = 536878673 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283408, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (259, 440, 800)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882)
[M::mem_pestat] mean and std.dev: (494.20, 350.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2423)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 356, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (365.35, 165.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677268 sequences (100000280 bp) in 3.837 CPU sec, 4.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282584, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 359, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (367.82, 165.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678490 reads in 220.511 CPU sec, 7.075 real sec
[M::mem_process_seqs] Processed 677922 reads in 134.980 CPU sec, 4.275 real sec
[M::process] read 676984 sequences (100000066 bp) in 2.323 CPU sec, 2.494 real sec...
sorting block 97, #reads = 1058598 mem = 536879985 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282252, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 362, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (371.33, 165.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676978 sequences (100000053 bp) in 3.570 CPU sec, 3.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282763, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 367, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (375.17, 166.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677268 reads in 216.869 CPU sec, 6.939 real sec
sorting block 98, #reads = 1060030 mem = 536880133 peak = 1048084480
[M::mem_process_seqs] Processed 676984 reads in 132.807 CPU sec, 4.200 real sec
[M::process] read 676442 sequences (100000139 bp) in 2.380 CPU sec, 2.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282616, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 367, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (375.20, 167.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 99, #reads = 1059338 mem = 536879423 peak = 1048084480
[M::process] read 676528 sequences (100000073 bp) in 3.527 CPU sec, 4.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282393, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 373, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (380.40, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676978 reads in 213.003 CPU sec, 6.845 real sec
[M::mem_process_seqs] Processed 676442 reads in 130.671 CPU sec, 4.145 real sec
[M::process] read 676282 sequences (100000041 bp) in 2.257 CPU sec, 2.361 real sec...
sorting block 100, #reads = 1059777 mem = 536880140 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282687, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 374, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (382.87, 172.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676018 sequences (100000116 bp) in 3.568 CPU sec, 3.892 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281432, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 371, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (379.22, 169.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676528 reads in 218.827 CPU sec, 7.006 real sec
[M::mem_process_seqs] Processed 676282 reads in 134.848 CPU sec, 4.261 real sec
[M::process] read 675720 sequences (100000022 bp) in 2.329 CPU sec, 2.380 real sec...
sorting block 101, #reads = 1061145 mem = 536880825 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282088, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (381.08, 168.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675444 sequences (100000105 bp) in 3.543 CPU sec, 3.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281777, 7, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 447, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339)
[M::mem_pestat] mean and std.dev: (376.20, 262.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1730)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (384.70, 171.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 173, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485)
[M::mem_pestat] mean and std.dev: (211.36, 94.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 590)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676018 reads in 215.976 CPU sec, 6.947 real sec
[M::mem_process_seqs] Processed 675720 reads in 132.938 CPU sec, 4.232 real sec
sorting block 102, #reads = 1061122 mem = 536878027 peak = 1048084480
[M::process] read 675406 sequences (100000225 bp) in 2.656 CPU sec, 2.774 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281505, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (388.39, 172.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674952 sequences (100000058 bp) in 3.120 CPU sec, 3.761 real sec...
sorting block 103, #reads = 1060015 mem = 536879824 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281265, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (388.26, 172.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 321, 562)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1314)
[M::mem_pestat] mean and std.dev: (334.40, 173.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1690)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675444 reads in 216.155 CPU sec, 7.066 real sec
[M::mem_process_seqs] Processed 675406 reads in 136.056 CPU sec, 4.390 real sec
[M::process] read 675302 sequences (100000261 bp) in 2.882 CPU sec, 3.260 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281848, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (392.99, 174.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 104, #reads = 1060736 mem = 536882176 peak = 1048084480
[M::process] read 674990 sequences (100000052 bp) in 2.966 CPU sec, 3.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 281865, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (263, 409, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (397.80, 194.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 382, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (391.20, 175.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674952 reads in 211.051 CPU sec, 6.828 real sec
[M::mem_process_seqs] Processed 675302 reads in 133.226 CPU sec, 4.284 real sec
[M::process] read 675210 sequences (100000014 bp) in 2.670 CPU sec, 2.829 real sec...
sorting block 105, #reads = 1061557 mem = 536878313 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281695, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (392.64, 175.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675356 sequences (100000191 bp) in 3.069 CPU sec, 3.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281132, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 384, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (394.62, 177.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1325)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674990 reads in 212.985 CPU sec, 6.847 real sec
[M::mem_process_seqs] Processed 675210 reads in 135.432 CPU sec, 4.306 real sec
[M::process] read 674980 sequences (100000278 bp) in 3.082 CPU sec, 3.220 real sec...
sorting block 106, #reads = 1060695 mem = 536879329 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281520, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 383, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (393.25, 177.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1324)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674946 sequences (100000038 bp) in 3.024 CPU sec, 3.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281258, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 389, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (398.80, 180.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1343)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675356 reads in 217.324 CPU sec, 6.957 real sec
[M::process] read 676544 sequences (100000271 bp) in 2.103 CPU sec, 2.130 real sec...
sorting block 107, #reads = 1061276 mem = 536878891 peak = 1048084480
[M::mem_process_seqs] Processed 674980 reads in 134.290 CPU sec, 4.253 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281697, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 390, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (400.55, 181.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 108, #reads = 1059481 mem = 536879084 peak = 1048084480
[M::process] read 679564 sequences (100000277 bp) in 3.579 CPU sec, 3.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281615, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 373, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (385.72, 177.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679632 sequences (100000275 bp) in 2.253 CPU sec, 2.259 real sec...
[M::mem_process_seqs] Processed 674946 reads in 232.272 CPU sec, 7.430 real sec
[M::mem_process_seqs] Processed 676544 reads in 151.582 CPU sec, 4.820 real sec
sorting block 109, #reads = 1057751 mem = 536880601 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282738, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 345, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (361.72, 170.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678932 sequences (100000256 bp) in 3.772 CPU sec, 4.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283377, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 237, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126)
[M::mem_pestat] mean and std.dev: (262.00, 161.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1448)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 347, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (362.90, 171.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 679564 reads in 214.828 CPU sec, 6.856 real sec
[M::process] read 678098 sequences (100000119 bp) in 2.136 CPU sec, 2.140 real sec...
[M::mem_process_seqs] Processed 679632 reads in 130.074 CPU sec, 4.118 real sec
sorting block 110, #reads = 1056256 mem = 536878129 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283247, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (363.98, 169.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677944 sequences (100000294 bp) in 3.683 CPU sec, 3.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282549, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 357, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (372.17, 173.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678932 reads in 216.022 CPU sec, 6.921 real sec
[M::mem_process_seqs] Processed 678098 reads in 132.990 CPU sec, 4.202 real sec
[M::process] read 677234 sequences (100000269 bp) in 2.692 CPU sec, 2.756 real sec...
sorting block 111, #reads = 1063644 mem = 536877375 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282786, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 362, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (375.93, 175.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676540 sequences (100000032 bp) in 3.224 CPU sec, 3.667 real sec...
sorting block 112, #reads = 1062729 mem = 536881770 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282223, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 365, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (376.73, 172.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677944 reads in 205.486 CPU sec, 6.643 real sec
[M::mem_process_seqs] Processed 677234 reads in 127.214 CPU sec, 4.066 real sec
[M::process] read 676626 sequences (100000077 bp) in 2.144 CPU sec, 2.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281498, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 369, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (380.07, 172.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 113, #reads = 1063480 mem = 536877842 peak = 1048084480
[M::process] read 676118 sequences (100000142 bp) in 3.336 CPU sec, 3.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281877, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 367, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (379.16, 173.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676540 reads in 209.429 CPU sec, 6.784 real sec
[M::mem_process_seqs] Processed 676626 reads in 128.723 CPU sec, 4.100 real sec
[M::process] read 676526 sequences (100000190 bp) in 2.758 CPU sec, 3.016 real sec...
sorting block 114, #reads = 1063169 mem = 536878877 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281211, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 373, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (383.25, 172.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676598 sequences (100000136 bp) in 3.078 CPU sec, 3.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282125, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 217, 217)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 293)
[M::mem_pestat] mean and std.dev: (193.23, 44.67)
[M::mem_pestat] low and high boundaries for proper pairs: (15, 372)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 370, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (379.78, 171.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676118 reads in 211.810 CPU sec, 6.855 real sec
[M::process] read 676330 sequences (100000099 bp) in 2.753 CPU sec, 2.973 real sec...
sorting block 115, #reads = 1063907 mem = 536879653 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281753, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 371, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (379.44, 169.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676598 reads in 111.185 CPU sec, 3.528 real sec
[M::process] read 676134 sequences (100000247 bp) in 2.732 CPU sec, 2.942 real sec...
[M::mem_process_seqs] Processed 676526 reads in 304.107 CPU sec, 9.732 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281584, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 368, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (378.21, 169.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 116, #reads = 1063773 mem = 536879413 peak = 1048084480
[M::process] read 676230 sequences (100000211 bp) in 3.296 CPU sec, 3.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281464, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 370, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (380.56, 170.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676330 reads in 228.822 CPU sec, 7.275 real sec
[M::mem_process_seqs] Processed 676134 reads in 126.693 CPU sec, 4.011 real sec
[M::process] read 676322 sequences (100000001 bp) in 2.708 CPU sec, 2.834 real sec...
sorting block 117, #reads = 1062009 mem = 536880831 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281998, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 371, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (381.39, 171.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677070 sequences (100000196 bp) in 2.931 CPU sec, 3.271 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281176, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 370, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (380.58, 171.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676230 reads in 216.376 CPU sec, 6.925 real sec
[M::mem_process_seqs] Processed 676322 reads in 136.105 CPU sec, 4.305 real sec
[M::process] read 676870 sequences (100000036 bp) in 2.566 CPU sec, 2.722 real sec...
sorting block 118, #reads = 1063551 mem = 536878397 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281914, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 361, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (372.75, 168.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 119, #reads = 1061857 mem = 536878440 peak = 1048084480
[M::process] read 676976 sequences (100000243 bp) in 3.359 CPU sec, 3.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282156, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 364, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (375.18, 170.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677070 reads in 211.623 CPU sec, 6.756 real sec
[M::process] read 676278 sequences (100000134 bp) in 2.381 CPU sec, 2.411 real sec...
[M::mem_process_seqs] Processed 676870 reads in 134.676 CPU sec, 4.249 real sec
sorting block 120, #reads = 1062371 mem = 536877022 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282290, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 364, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (375.14, 169.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675782 sequences (100000258 bp) in 3.102 CPU sec, 3.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281717, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 526, 890)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2368)
[M::mem_pestat] mean and std.dev: (552.10, 395.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3107)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 372, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (383.39, 173.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676976 reads in 215.450 CPU sec, 6.882 real sec
[M::process] read 675480 sequences (100000175 bp) in 2.212 CPU sec, 2.222 real sec...
[M::mem_process_seqs] Processed 676278 reads in 136.489 CPU sec, 4.308 real sec
sorting block 121, #reads = 1062215 mem = 536878722 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281757, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 380, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (389.96, 176.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675620 sequences (100000083 bp) in 3.478 CPU sec, 3.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281241, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 381, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (390.75, 176.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675416 sequences (100000103 bp) in 1.952 CPU sec, 1.954 real sec...
[M::mem_process_seqs] Processed 675782 reads in 216.056 CPU sec, 6.899 real sec
[M::mem_process_seqs] Processed 675480 reads in 132.955 CPU sec, 4.208 real sec
sorting block 122, #reads = 1060273 mem = 536881183 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281888, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (389.29, 174.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675366 sequences (100000029 bp) in 3.002 CPU sec, 3.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282176, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (390.76, 175.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675540 sequences (100000003 bp) in 2.259 CPU sec, 2.268 real sec...
[M::mem_process_seqs] Processed 675620 reads in 214.695 CPU sec, 6.853 real sec
sorting block 123, #reads = 1060658 mem = 536879869 peak = 1048084480
[M::mem_process_seqs] Processed 675416 reads in 129.490 CPU sec, 4.089 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281266, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 1068, 1068)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2704)
[M::mem_pestat] mean and std.dev: (710.60, 505.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3522)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (393.08, 177.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 124, #reads = 1061528 mem = 536881026 peak = 1048084480
[M::process] read 675992 sequences (100000278 bp) in 3.806 CPU sec, 4.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281486, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 380, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (391.93, 179.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675366 reads in 216.602 CPU sec, 6.911 real sec
[M::mem_process_seqs] Processed 675540 reads in 133.817 CPU sec, 4.225 real sec
[M::process] read 675688 sequences (100000015 bp) in 2.853 CPU sec, 2.940 real sec...
sorting block 125, #reads = 1060898 mem = 536876385 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281868, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 374, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (388.78, 180.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680792 sequences (100000187 bp) in 3.207 CPU sec, 3.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 281586, 1, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 311, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (221.27, 92.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 382, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (394.68, 181.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1338)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675992 reads in 229.120 CPU sec, 7.313 real sec
[M::process] read 681556 sequences (100000177 bp) in 2.206 CPU sec, 2.206 real sec...
[M::mem_process_seqs] Processed 675688 reads in 142.317 CPU sec, 4.498 real sec
sorting block 126, #reads = 1059549 mem = 536878810 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283451, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 335, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (352.12, 166.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681916 sequences (100000069 bp) in 4.064 CPU sec, 4.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284160, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (344.85, 161.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680856 sequences (100000061 bp) in 2.487 CPU sec, 2.495 real sec...
[M::mem_process_seqs] Processed 680792 reads in 243.326 CPU sec, 7.732 real sec
[M::mem_process_seqs] Processed 681556 reads in 143.299 CPU sec, 4.531 real sec
sorting block 127, #reads = 1053923 mem = 536875892 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284514, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (342.11, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 280, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (106, 396)
[M::mem_pestat] mean and std.dev: (246.00, 50.43)
[M::mem_pestat] low and high boundaries for proper pairs: (44, 454)
[M::mem_pestat] skip orientation RR
sorting block 128, #reads = 1060217 mem = 536877907 peak = 1048084480
[M::process] read 681266 sequences (100000286 bp) in 3.442 CPU sec, 3.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283613, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (346.28, 158.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 840, 894)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2208)
[M::mem_pestat] mean and std.dev: (606.20, 430.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2865)
[M::mem_pestat] skip orientation RR
[M::process] read 680832 sequences (100000071 bp) in 2.404 CPU sec, 2.413 real sec...
[M::mem_process_seqs] Processed 681916 reads in 240.553 CPU sec, 7.681 real sec
[M::mem_process_seqs] Processed 680856 reads in 146.572 CPU sec, 4.631 real sec
sorting block 129, #reads = 1058065 mem = 536877735 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284133, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (344.92, 160.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679446 sequences (100000300 bp) in 3.842 CPU sec, 4.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283688, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (345.44, 157.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678100 sequences (100000283 bp) in 2.511 CPU sec, 2.516 real sec...
[M::mem_process_seqs] Processed 681266 reads in 234.658 CPU sec, 7.451 real sec
[M::mem_process_seqs] Processed 680832 reads in 151.202 CPU sec, 4.780 real sec
sorting block 130, #reads = 1058453 mem = 536880492 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282905, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 346, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (356.01, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677928 sequences (100000081 bp) in 3.579 CPU sec, 3.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282350, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 227, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (100, 365)
[M::mem_pestat] mean and std.dev: (219.73, 24.43)
[M::mem_pestat] low and high boundaries for proper pairs: (47, 418)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 355, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (364.79, 164.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677736 sequences (100000252 bp) in 2.340 CPU sec, 2.341 real sec...
[M::mem_process_seqs] Processed 679446 reads in 234.255 CPU sec, 7.458 real sec
[M::mem_process_seqs] Processed 678100 reads in 141.022 CPU sec, 4.471 real sec
sorting block 131, #reads = 1056738 mem = 536879718 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282954, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 359, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (368.66, 166.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677644 sequences (100000254 bp) in 3.902 CPU sec, 4.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282839, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 361, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (370.30, 166.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677928 reads in 226.058 CPU sec, 7.200 real sec
[M::process] read 677362 sequences (100000187 bp) in 2.419 CPU sec, 2.429 real sec...
sorting block 132, #reads = 1059096 mem = 536881954 peak = 1048084480
[M::mem_process_seqs] Processed 677736 reads in 134.451 CPU sec, 4.241 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282695, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 363, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (372.52, 168.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 133, #reads = 1060489 mem = 536877063 peak = 1048084480
[M::process] read 677166 sequences (100000058 bp) in 3.493 CPU sec, 3.757 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282613, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 364, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (372.69, 167.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677644 reads in 225.410 CPU sec, 7.210 real sec
[M::mem_process_seqs] Processed 677362 reads in 139.527 CPU sec, 4.397 real sec
[M::process] read 676866 sequences (100000087 bp) in 2.557 CPU sec, 2.598 real sec...
sorting block 134, #reads = 1060182 mem = 536878964 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281836, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 366, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (376.18, 170.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676618 sequences (100000053 bp) in 3.804 CPU sec, 4.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281393, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 369, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (377.96, 170.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677166 reads in 228.508 CPU sec, 7.279 real sec
[M::process] read 676268 sequences (100000058 bp) in 2.336 CPU sec, 2.345 real sec...
[M::mem_process_seqs] Processed 676866 reads in 138.167 CPU sec, 4.359 real sec
sorting block 135, #reads = 1060655 mem = 536879815 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281894, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 373, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (381.39, 170.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676150 sequences (100000017 bp) in 3.585 CPU sec, 3.849 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281128, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 373, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (381.58, 170.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676618 reads in 228.589 CPU sec, 7.301 real sec
[M::process] read 676182 sequences (100000009 bp) in 2.407 CPU sec, 2.431 real sec...
[M::mem_process_seqs] Processed 676268 reads in 138.458 CPU sec, 4.365 real sec
sorting block 136, #reads = 1059679 mem = 536878146 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280844, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 373, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (380.08, 167.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 137, #reads = 1060688 mem = 536878500 peak = 1048084480
[M::process] read 675632 sequences (100000068 bp) in 3.914 CPU sec, 4.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281862, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (382.98, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (455, 516, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (333, 638)
[M::mem_pestat] mean and std.dev: (489.82, 35.26)
[M::mem_pestat] low and high boundaries for proper pairs: (272, 699)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676150 reads in 229.710 CPU sec, 7.322 real sec
[M::process] read 675320 sequences (100000248 bp) in 2.323 CPU sec, 2.330 real sec...
[M::mem_process_seqs] Processed 676182 reads in 137.138 CPU sec, 4.328 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281190, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (386.14, 171.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 138, #reads = 1060262 mem = 536876791 peak = 1048084480
[M::process] read 675184 sequences (100000248 bp) in 3.286 CPU sec, 3.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280586, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 389, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (321.80, 145.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (388.99, 171.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 1228, 1228)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3196)
[M::mem_pestat] mean and std.dev: (730.90, 499.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4180)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675632 reads in 220.474 CPU sec, 7.033 real sec
[M::process] read 675210 sequences (100000131 bp) in 2.423 CPU sec, 2.431 real sec...
[M::mem_process_seqs] Processed 675320 reads in 136.743 CPU sec, 4.317 real sec
sorting block 139, #reads = 1060920 mem = 536878868 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281271, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (390.95, 171.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675396 sequences (100000051 bp) in 3.368 CPU sec, 3.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280813, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (390.44, 172.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675184 reads in 213.840 CPU sec, 6.826 real sec
[M::mem_process_seqs] Processed 675210 reads in 133.836 CPU sec, 4.225 real sec
[M::process] read 674926 sequences (100000077 bp) in 2.668 CPU sec, 2.706 real sec...
sorting block 140, #reads = 1060932 mem = 536880670 peak = 1048084480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281462, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (386.79, 170.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674832 sequences (100000073 bp) in 3.309 CPU sec, 3.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281170, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 381, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (389.77, 172.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675396 reads in 211.094 CPU sec, 6.736 real sec
[M::process] read 480600 sequences (71134468 bp) in 1.938 CPU sec, 1.939 real sec...
sorting block 141, #reads = 1060945 mem = 536883287 peak = 1048084480
[M::mem_process_seqs] Processed 674926 reads in 129.752 CPU sec, 4.102 real sec
sorting block 142, #reads = 1061351 mem = 536880093 peak = 1083215872
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280863, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (391.48, 172.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 200100, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 375, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (385.89, 172.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674832 reads in 169.480 CPU sec, 5.517 real sec
[M::mem_process_seqs] Processed 480600 reads in 90.851 CPU sec, 2.943 real sec
sorting block 143, #reads = 1062250 mem = 536880105 peak = 1083215872
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 152598822
overall: 69835665408 mem 32737.166 user 848.584 sys 1124.912 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.L006\tPU:L006\tSM:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tLB:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R1.fastq.gz FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1124.954 sec; CPU: 33585.754 sec
sorting block 144, #reads = 247098 mem = 125574675 peak = 1083215872
uniq header count 1
merging 145 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8971409724
output reads: 153289289
bam_mem_sort: 145 calls 127.644 user 2.751 sys 131.381 real
bam_write: 145 calls 106.479 user 5.287 sys 156.344 real
execute: 1 calls 86.945 user 5.638 sys 1218.692 real
merge_files: 1 calls 86.883 user 5.591 sys 93.587 real
parse_chunk: 2308 calls 114.471 user 34.568 sys 163.974 real
read_chunk: 6228 calls 65.124 user 31.348 sys 880.233 real
sort_block: 1 calls 38.659 user 30.281 sys 842.849 real
write_chunk: 3918 calls 86.260 user 4.977 sys 92.007 real
overall: 3155881984 mem 2744.192 user 120.208 sys 1218.721 real
ls: cannot access '*.cram': No such file or directory